NL-03-2523, NPDES Permit Renewal Application

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NPDES Permit Renewal Application
ML040070434
Person / Time
Site: Vogtle  Southern Nuclear icon.png
Issue date: 12/15/2003
From: Gasser J
Southern Nuclear Operating Co
To:
Document Control Desk, Office of Nuclear Reactor Regulation
References
NL-03-2523
Download: ML040070434 (87)


Text

Jeffrey T. Gasser Southern Nuclear Vice President Operating Company, Inc.

40 Inverness Center Parkway Post Office Box 1295 Birmingham, Alabama 35201 Tel 205.992.7721 Fax 205.992.0403 O

SOUTHERN COMPANY December 15, 2003 Energy to Serve Your World" Docket Nos.:

50424 NL-03-2523 50425 U. S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D. C. 20555-0001 Vogtle Electric Generating Plant NPDES Permit Renewal Application Ladies and Gentlemen:

In accordance with Section 3.2 of the Vogtle Electric Generating Plant Environmental Protection Plan (Units I and 2), Appendix B to Facility Operating License Nos. NPF-68 and NPF 8 1, enclosed is a copy of the permit renewal application package for National Pollutant Discharge Elimination System (NPDES) Permit No. GA0026786 submitted to the Georgia Environmental Protection Division.

This letter contains no NRC commitments. If you have any questions, please advise.

Sincerely, Je e T.asser JTG/KWK/daj Enclosure cc:

Southern Nuclear Operating Company Mr. J. D. Woodard, Executive Vice President Mr. W. F. Kitchens, General Manager - Plant Vogtle Mr. M. Sheibani, Engineering Supervisor - Plant Vogtle Mr. G. D. Elmore, Environmental Services Mr. W. C. Carr, Environmental Services (wv/o attachments)

Document Services RTYPE: CVC7000 Georgia Power Company Mr. M. E. Wilder, Environmental Affairs (w/o attachments) clD(

U. S. Nuclear Regulatory Commission NL-03-2523 Page 2 U. S. Nuclear Regulatory Commission Mr. L. A. Reyes, Regional Administrator Mr. S. D. Bloom, NRR Project Manager - Vogtle Mr. J. Zeiler, Senior Resident Inspector - Vogtle State of Georgia Mr. L. C. Barrett, Commissioner - Department of Natural Resources

Southern Nuclear Operating Company, Inc.

  • k Post Office Box 1295 Birmingham, Alabama 35201 1295 Tel 205.992.5000 SOUTHERNAK Energy to Serve Your World File: E.03.07 Log: EV-03-2479 December 4, 2003 FEDERAL EXPRESS Voqtle Electric Generating Plant (NPDES Permit No. GA0026786)

Permit Renewal Application Package Mr. Michael S. Creason Unit Coordinator Water Protection Branch Environmental Protection Division 4220 International Parkway, Suite 101 Atlanta, Georgia 30354

Dear Mr. Creason:

Attached is the NPDES permit renewal application package for the Vogtle Electric Generating Plant (NPDES Permit No. GA0026786) located near Waynesboro, Georgia. The renewal package contains completed EPA Forms 3510-1 and 3510-2C. Also included are the results from whole effluent toxicity (WET) chronic testing, as required by the State of Georgia.

If you have any questions or need additional information, please contact Greg Elmore at (205) 992-5264.

Sincerely, Wayne C. Carr, Manage A

Environmental Services WCC/GDE:ahl Enclosure

EV-03-2479 Mr. Michael S. Creason State of Georgia Environmental Protection Division Page Two bcc:

w/o Enclosure J. T. Gasser P. D. Rushton N. J. Stringfellow

1. A. Kochery B. D. Carter M. E. Wilder B. R. Evans A. Harris w/ Enclosure W. F. Kitchens C. M. Burke SNC Document Management - Vogtle (Return Receipt)

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-j; Application for Permit Renewal NPDES Permit No. GA0026786 Vogtle Electric Generating Plant Submitted by Southern Nuclear Operating Company December 2003 SOUT#4LftN k.

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.Atbeh to thtiasppiircaton a opgraphic map 0f Ui amra extending to atleast onc miloebeyond proport ybhunderiThe mnap mustM t

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l ocation of each o it-existing aend proposed intake and discharge s Mc utes~eachoiW iha a dou t eatnt nstorge-or di~

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PM NATURE OFD1USINESS (-*f*de Generation of electricity through the use of nuclear fuel.

Vogtle Electric Generating Plant is jointly owned by Georgia Power Company, Oglethorpe Power Corporation, Municipal Electric Authority of Georgia, and the City of Dalton, Georgia.

u.PU.CERTIFICATION fsee /0Sh'z'-tbM~

~~6Afr~~~n~~'Ini~hftit&Jcg4JMV4&/7 7 A. NAME &OFFICIAL TITLE (b'0; opnd7Q JB. SIGN TUR C. DATE SIGNED J. T. Gasser, Vice President b/

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EPA Form 3510-1 (8-90)

Attachment to EPA Form 1,Section X.E Existing Environmental Permits Vogtle Electric Generating Plant Permit Name Permit Number Held By NPDES Permit GA0026786.

_ Southern Nuclear Operating Co NPDES General Stormwater Permit GAROOOOOO Southern Nuclear Operating C_.

NPDES General Construction Storrnwater GARI0000I Southern Nuclear Operating Co Permit Public Non-Transient Non-Community PG0330017 Georgia Power Company Water System Public Transient Non-Community Water NG0330036 Georgia Power Company System Public Non-Transient Non-Com-munity PG0330035

-- -Georgia Power Company Water System Haz. Materials Certificate of Registration 061603 001 014L Southern Nuclear Operating Co (Issued by U.S. Dept. of Transportation)

Dredging Permit 950004490 Southern Nuclear Operating Co (Issued by U.S. Army Corps of Engineers)

Surface Water Withdrawal 017-0191-05 Southern Nuclear Operating Co Ground Water Use 017-0003 Southern Nuclear Operating Co Solid Waste Handling-Permit.-

01-06Dj)I(X) No. 2 Georgia Power Company Solid Waste Handling Permit 017-007D(L)(1)

Georgia Power Company Solid Waste Collection Permit By Rule PBR-0 I 7-07COL Southern Nuclear Operating Co Part 70 Operating Permit 4911-033-0030-V-01-0 Georgia Power Company/Southern Company GA0026786 Page I of I

4 A

EPA FORM 2C NPDES Application for Permit to Discharge Wastewater Consolidated Permits Program Vogtle-Eiectric Generating Plant

-NPDES No. GA0026786 Siubmiued by Southern Nuclear Operating Company December 2003 SCUl.MP&NA

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l Please print or type in the unshaded areas only. I EPA LD.(coqyfom Atem /of~orm I)

GA0026786 I

Form Approved.

OMB No. 204D-0086 Approval expires 7-31488

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APPLICATON FOR PP ETTO DISCHARGE WASITWATER + H S %

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09 SAVANNAH RIVER 14-15 33 08 30 81 45 44 BEAVER DAM CREEK A LOYWS, SOURCES OF POLL ION AN) TREAMNT TEaNOLOGIES _

A:Attach a line drawing showingthe watr flow through th facility. Indiat souCees of intake waetr-operations conrnbuting,wastewater to h efluaitti vcfandt ntalert initslabel tocors Uimore defedd pnsiniteB. Costiaie aceonheiing

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STREAMS UNITS I & 2) 002 UNIT I COOLING TOWER 10,000 GPM 2F 4A BLOWDOWN (MAXIMUM) 002A UNIT I EMERGENCY 2F 4A OVERFLOW TO STORM DRAINS 003 UNIT 2 COOLING TOWER

,I Q00 GPM 2F 4A BLOWDOWN (MAXIMUM) 003A UNIT 2 EMERGENCY 2F 4A OVERFLOW TO STORM DRAINS 004 UNIT I WASTE WATER 1,300 GPM AA RETENTION BASIN (MAXIMUM) 005 UNIT 2 WASTE WATER 1,300 GPM 4A RETENTION BASIN (MAXIMUM) 006 SEWAGE TREATMENT PLANT

. 2F, 3A 4A EMERGENCY OVERFLOW 007 LIQUID RADWASTE SYSTEMS 100 GPM 2J 4A DISCHARGE (UNIT I)- --

(MAXIMUM) 008 LIQUID RADWASTE SYSTEMS

.00 GPM 2J 4A DISCHARGE (UNIT 2)

(MAXIMUM) 009 NUCLEAR SERVICE COOLING 500 GPM 2F 4A TOWER BLOWDOWN (UNITS I (MAXIMUM)

&2) 010 RADWASTE DILUTION FLOW 4A TO OUTFALL 001 011 INTAKE SCREEN BACKWASH

.4A 012 POWER BLOCK STORM DISCHARGE FROM THIS 4A WATER DISCHARGE TO OUTFALL IS GOVERNED BY SAVANNAH RIVER GENERAL PERMIT NO.

GAR000000 INTERMITTENT FLOW (SEE ATTACHED TABLE)

DMCL USE ONLY (effluent guidelines sub-categories);.

EPA Form 3S10-2C (Rev.2-85)

PAGE 1 0F4 CONTINUE ON PAGE 2 EPA Form 3510-2C (Rev.2-85)

PAGE IOF 4 CONTINUE ON PAGE 2

Please print or type in the unshaded areas only. I EPA LD.(copyfrom item Jo/JForm GA0026786 "I

Form Approved.

OMB No. 2040-0086 Approval expires 7-31-88

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M'PPU1C-A9N FORlERMIT TO'DISCHAIRGE WASTEWA7TR.-42~,rl1;~,~.-~-.t:'~

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IFLOWS, OURCES OF POLUM~ON. AND TREATMENT TECHNOLOGEES,: ~

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013 INTAKE STRUCTJ UR ST UK ORM --

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GAROOOOOO 4A 014 EAST STORM WATER DISCHARGE FROM THIS 4A DISCHARGE TO BEAVER DAM OUTFALL IS GOVERNED BY CREEK (RETENTION POND I)

GENERAL PERMIT NO.

GAR000000 015 WEST STORM WATER DISCHARGE FROM THIS 4A DISCHARGE TO BEAVER DAM OUTFALL IS GOVERNED BY CREEK (RETENTION POND 2)

GENERAL PERMIT NO.

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rarns whichmay affect the discharges descnbed in ts appiation? :tis

irnclcdes, buiis not limited to nit co ditionsdmintstrative orenforcementorers, ennforcemat compliance scheduklen stipulationis,c6urt ordcrs,'

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a _REQUIIED. L : PROJECTED. I B. OPTIONAI You may attach additional sheets describing any. additional water pollution control programs (or other envirrnmental projects ihichmay afect your discharges) you now have underway or wvhich you plati Indicate whether each program is now underway or pned;ano indicateyouractual

.or planred seIC5es for construction;:

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-. ARKI"D FDESCRIMNONOFADDInONA LCONTROL PROGRAMISA1TACflE

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EPA Form 3510-2C (Rev. 2485)

PAGE 2 OF 4 CONTINUE ON PAGE 3

EPA J.D.NUMBER (covpyvomIemIJ FormI)

Form Approved.

GA0026786 O

0MB No. 2040-0086 CONTINUED FROM PAGE 2 Approval expires 7-31-88 rV,-JNTAKEANDEFLUTCHARA S'lCS:-'4..-3.:%~,'.!.-.".:-i I

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D.Use thespace belowto list anyof thepollutants listed inTable2c3if the instructions;,.vhichyou knowor have reasonto believe is discharged ormay;.-

,i bedisiSared rni'any bui afl.'.For every pollutant you list, briefy debithi rcasoisyou bdiev it to bc priscnt and rcnn any 31yric21 datn in.2 ssion ) _i.

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  • None VI. POTENIIAL DISCHARGES NC COVERED BY ANALYSIS * }

-s anypollutantlisted intemV-Casutaceor a component of a substance which you currently use ortnanuflcturc s an intermediate or final productao N/A EPA Form 3510-2C (Rev. 2-85)

PAGE 3 OF 4 CONTINUE ON PAGE 4

CONTINUED FROM THE FRONT VII. BIOLOGICALTOXCITY TESTING DATA':;

Do you have any knowledge or.reason to believe that any biological test foracute or dronic toxkity has bn made on any of yourdischarges orona receivmg wterin e aiont'y uri h'rg ithin t el ty

a -~-s. ji5.k.al
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ES (identify the test(s)and-describe theirpusrps 5 :,II.;1; 1NO (go to S WHOLE EFFLUENT TOXICITY (WET) CHRONIC TESTING PERFORMED IN NOVEMBER 2003 AS REQUIRED BY GEORGIA EPD TO SUPPORT NPDES PERMIT RENEWAL. TESTING RESULTS ARE INCLUDED WITH THIS RENEWNAL APPLICATION.

Viii CONTRACT A}IALYS1S INFOR.MATION ~~~~~~~'

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Were any offteanalyses rportea In Item V Pertormed by a contact laboratoiyrcora'];nhrnv,-

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polltants anayi~d by;cach such laboruoi, or' firmn beo)r;~:~4~

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,.ADDRESSL?,

-,1.,7C.TE1LEPH0N4E-;~'

D.POLUFANTSANLZD Alabama Power Company P.O. Box 2641 (205) 664-6182 All except pH, temperature, Total General Test Laboratory Birmingham, AL 35291 Residual Chlorine, and Radiological Florida Radiochemistry 5456 Hoffner Ave.

(407) 382-7733 Radiological Services, Inc.

Suite 201 Orlando, FL 32812 BioTox Laboratory 3200 Town Point Dr., NW (770) 421-7027 Whole Effluent Toxicity Testing MACTEC Eng. & Consulting Suite 100 Kennesaw, GA 30144 IX; CERTIFICATION Jcertrfy underpenalyoJ0/aw that this documentand allttachments wereprepared undermy direction orsupervision in accordance:

with a system designatedto essure ihat ua4/ 7edpersonnd perlygather and evaluate the

<died. ased on ry.:

inguiry of/theperson orpersons who manage ihe.ysfen ar thosepersons directl; retsponsibleforg atheri'ighe information, the informadonosubmittedir / othebestof my nowldgedandlbelkf true; accurate, anddcompieie.. I am ewareih'atithere aresigniIC7cant t

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penaatiesor.rubrnmitinIOatse injormnatlon, incuding Ineposzoulp oiJrne onaimprisonmenriorAnow!n2 vioItaons.

A. NAME & OFFICIAL TITLE (type or print)

B. PHONE NO. (Area code & no.)

J. T. GASSER, VICE PRESIDENT (205) 992 -5000 C. SIGNATURE D. DATE SIGNED 4 A wav-1 1 JJO EPA Form 3510-2C (Rev. 2-85)

PAGE 4 OF 4

I.

9.

EPA FORM 2C SECTION V Intake and Effluent Characteristics Consolidated Permits Program Vogtle Electric Generating Plant NPDES No. GA0026786 Submitted by Southern Nuclear Operating Company December 2003 SOUIMLnNA COPAPAWO

PLEASE PRiNT ORTYPE IN THE UNSIIADED AREAS ONLY. You may report someor all ofthis inrorfnation on separate sheets (Use the someformrla) instead of completing these pages.

SEE INSTRUCTIONS.

V;. ITAKE:AND EFFLUlNT C-T.

lRCH ERA STICS (cotinued from page.3oFForm 2 PART A - You must provide the results of at least one analysis for every pollutant in this table. Complete i Form Approved.

OMB No. 2040-0086 d.Fecal Colilfom e.Fltoride(16984 48 8)

. Nitrate Nitrite ns. l9 i EPA Form 35t0-2C (Rev. 2-85)

ITEM V-n Cj..,.AtAN mlv

_h p I4O-L~

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g.

Nitrog c;

x*

Total Organic(a X

h. Oil and Greasec
1. Phosphorus (as P) ;4 Total (7723 i4-0)
.x 1;.Raditactivit' "
(2) Beta, Total - -

(3) Radiuxn.Jbtal.-,

x (4) Radium.226, t

~~~x

k. Sulrates 50; SO.
1. Sulfde (as SO.r,-'

l i4265 45-3)9-X

.m..Sulfite (dasSOj)7\\ :'

(!4265-45 3).

f:--

x

i. Surahctantsa x

O. Aluminium,.---,.--,

Total (i440 39 3)

p. Banum, Totalu;1 q:Boron, Total -

r, (1440 42.8)x' :

X r; Cobalt, Total (7440-48 4j t ST ron,.Total,

(7439-89.6)s4.;

r<

X tzMagnesium Totai (7439-95 4) '

u. Molybdenum,: -

-Total (7439 9h-7)

.v. Manganese,

,.Totl 7439-96-5)

X w aTinj Total -

x. Titaniu'm,..-..

-Tofaii(t440-32-6).

EPA Form 3510-2C (Rev. 2-95)

EPA I.D. NUMBER (copyfrom Item I of Form l)

OUTFALL NUMBER I

GA0026786 I

001 Form Approved.

OMB No. 2040-0086 Approval expires 7-31-88 CONTINUED FROM PAGE 3 OF FORM 2-C PART C-If you are a primary industry sod this outrsll contains process wastewater, error to Table 2c-2 in the nstructions. lo t.tetnrmine whichttaf tho (ICIMS fractions You maul testfor. Mask 'X'in column 2-for all uch G luMS firactios that apply to yourindustr,' nd forALL toic itia, cyanides. sdtotalphenols. If uarenotrequiredto na tcolumn2-a(secondaryiindustiesunonpcessws tewater ufaa no reqir ti C iS ftiont),mr t.' it 1ut 2-bfotr ahpolutt tyu owor hver o toeie e s prent Nurk a"in colum 2-oor each -

pollutant you believe is absent Ifyou mark column 2a for any pollutant, you must providetheresults of atleat one anal sis fort tatpoillutant, fyouma coun2 rith pou t you must

{provide thercit ofat les n

asi forthep ltutunt if you nwor tveas10t.to believe it vilt be discharged in conettions of to ppb or greatet. If you murk column 2b for acrtin, astylonitrile, 2.4 dinitrophenol. or 2-methyl-4i, 6 dioitropbtieol, y m tal pirovIe the tesus of at least ou anlsis for e: ftee poluants.

ihh y n o or hVe o tO bleve thtlyoudischargein conentrationi oflt pphorgreater.

therwise fo polatans forwhich you tro colur 2h, you moot etd e submit at trout on analsi obreNyd te ret or t polua nt se I he iscier Notth there

-rs opg dothi pajtple re iew ainch c ar Cumplete one table (all I pages) or ecoutfa.See sutions r additional details and uirem ent.

I.

POLLUTANT

2. MARK X ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ 1
3. EFFLUENT

______4.

UNIT 2

5. NTA KCE (o

ti)

,,~

ANUCS i

a Test-

b. Be-
b. Bet-
0. MAXIMUM DAILY b.MAXIMUM 30 DAY
6. LONG TERM AVRG d.NO.

NUMOER iso loved ieved VALUE VALUE (lrvlol)VLE

-. O

~AVERAGE-VAU b.ANAL-(Ifavaislble)

Reuid Erent Absent (I) CONCEN.

(2) MASS (T) CONCEN-(2) MASS (I) CONCEN-(2) MASS YSES TAI MASS (0 CONCE

( MASS METALS, CYANIDE, AND TOTAL PHENOLS N

T

T:

I:.

I N4. Antimony, I

00 mg/I lb/day

<0.0 121 Total (1440-36-0)

X X

<0.012 2M. Arsenic, Total (7440-38-2)

X X

<0.005 1

mg/l lb/day

<0.005 3M. Beryllium, 00II mg l/a 00II Total, 7440-41-7 X

X

<0.001 mg/I lb/day

<oool I

4M. Cadmium, Total:(7440-43-9)

X X

<0.002 1

mg/I lb/day

<0.002 1

SM. chromiumn Total (7443-47-3):; t ;

X X

0.001 I

mg/I lb/day

<0.001 6M. Coper Total X

0.006 mg/

b/day

<0.005 7M. Lead, Tolal X

X

<0.004 1

mg/I lb/day

<0.004 SM Mercury, Total (7430-97-06) 0 X

X

<0.004 1

mg/I lb/day

<0.0040 (M. Nickel, Total I 40M.

.elenI X

I X

<0,004 mg/I lb/day

<0.004 I:IM. Silver, Total (7440-22-4)

~~X X

<0.0064 mg/I lb/day

<0.006 12M.SeThalium, X<007_____

Total (7440-:28-0):

X X

<0.005 mg/I lb/day

<0.007 13M. Zinc8Total4 X

H(A7440 6-6)

X0393mg/

lb/day 14M. Cyanide, X

X

<0.005 mg/l lb/day

<0 005 15M.: Phenols, :- :;;

X

< 0.01 I

mg/I lb/day

<001

oa
2,3,7, 8-Te tra-: i:SX DEC lEREUT

.chlorodibenzo-P-OSCIERSUT EPA Fat (5712-2C(e.

-5 rg EPA Form 3510-2C (Rev. 2-95) rage V-3

.POLLUTANT, O L M2 RIX-. :. -. I A

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x lb/day

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<0.0020 lb/day I <0.0010 lb/day I <0.0010 lb/day i <0.0020 lb/day ! <0.0010 lb/day

<0.0020 lb/day

<0.0010

,1 -

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x Clflom~~Ohylvinyl' x

1 IV. Chloroform. I v

(676 63) tr rd,

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x 1l2V. Dclrboo; 1methane (75;71.8) -74.,:'

x AM3V Dichltor6diqufluto.'-,

I methan (~ I lb/day

\\.

I hanE x

lb/day IlV 1,2Di' ro clhtind'(1 07-06 ~ ;-2); ;

x lb/day

[-I 6_VA1I-D-Di thlr.

I -IfMf (7~~:354i 41:2 x

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,~I.

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1 8V,..13Dchoo tI:

ropylne(576 6.K Ib/day x

1 9V; 1!thylbenzenc t

lb/day x

20Y; Methyl Bromide.;::>

~ (14iV ~-2.

lb/day x

t IVMiMethyl Chldridqe:t;.'.'

lb/day x

EPA Form 3510-2C (Rev. 2-85)

CONTINUED FR~OM PAGE V.4

~~I.POLLUTANT'~>.r

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~~ 1A~JDCAS

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(2)MA.SS, A(),"

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c GC/NtS FRACTION-:

VOLATILE COMPOUNDS (continudl-

~~:

~

v-22V.Mtbln Chlonide, 002 23. 1M,2 er)h~r eh n x

<0.0020 (72-4V ex xroty~n <0.0020 luene (108-88

~~~~xX

<0.0020 26V; A,2-Trahs.-Dich~qoro-27V 1256.61 I -Triobor0etIan 116 5) ~~~T, x X

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X

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3 V., VinyI Chloride. (75 01.4)

~___

GCiMS FRACTION-'ACID COMPOUNDS,:.

~".-4
','~.

I A." 2-Chloriopheriol (55

)X

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X

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~~X X

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X

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X

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x X

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~~.

X X

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EPA Fnrm

CONTINUED FROM FRONT

---,IPOLLUTANT AND CAS, -e GCCMS FRACTION -BASE(NEUTR, IB; Acenaphthene

.3B3.Ahthracene6 (120.1 2-7),

  • 4B.; Benizidine(2.87.5)

'5B:.Berzo(d), A~nthraceine.--

,6B, Benzo (a).Pyrehbe.4

,7B.3,4-Bdnzo-fluotanthefib i8B.,Bento ONir) Peyrn~

  • 9B3;.BEenzd (k)Fluoranthende~.

I121. flis 2h§ r

12B.'4rm~ey

~

Phn ther 1 21605:

1 151 ButylBenzyll 1613, 2-Chlotbnlphthakfene:

Phenyl Ether"(76005;7213)

X 1813; Chrysene (2,18 01-9)::.

.19B; Dibenzo (a I)

Anthir-cen (53 70 3) bezenc (95-50 I 21B. 1,3 Dihloo-'

bnien"e(541 73jA

. l.tt'f !i EPA Fnrm 3510.2C (Rev. 245)

Psge V-6

0 EPA I.D. NUMBER (capyfrom Item I of OUTFALL NUMBER.

Form Approved.

Form I)

OMB Mo. 2040-0086 CONTINUED FROM PAGE V-6 G001 0 pproval epres 7-31-88

~~~~~GA0026786J_ ____Apoaepre7-18 z^,,

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GCfMS FRACTION:- BASE/NHUTRAL COMPOUN~DS(of~e~~~

2213 1,4 Thchlc~~~~robeXn<z0044 mg/I lb/day

<0.0044 238.B.3;3-Dichlorobenzidine~e- ;-

231-i.3 3\\_ D~cblobetiznf X

s r tX ;

.X X

<0.0165 I

mg/I lb/day

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l htb.l-4t.

X X

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mg/l lb/day

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X X

<0.00216 mg/Il lb/day

<0.0016

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X

<0.0057 1

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f v. t; ; -

X X

<0.0025 1

mg/l lb/day

<0.0025 I

29B..Di-N.Oetyl~~hlhalate X

X

<0.0025 1

mg/I lb/day

<0.0025 1

.30B-; I j2Diphenyl hydraie,

.30b;Z1,2-Dlphenyl-hI22a66ne X

X

<0.003 1

mg/l lb/day

<0.003 I

(M~zobenze (I

(22-66'7 311B. Fluoranthene (206-44 0)

X X

<0.0022 A

1 mg/

Ib/day

<0.0022

  • ,32B.Fljorene (86.73 7)

.=

X X

<0.0019 I

mg/l lb/day

<0.00 19

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X X

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<0.0019 I

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CONTINUED FROM FRONT L' POLLUTANT

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^

X X

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lb/day

<0.0054 8P:45 4

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<0.0019 I

mg/l lb/day

<0.0019 1

.46Th.1,2,4-TrizU, s

-X

<0.00 19 mg/l lb/day

<0.0019 GNIMS FRACTION-PESTICIDES:,;4o--

Ax1:

IP. Aldrin (309.00)

<0.003

.I mg/l lb/day

<0.003 I

__X <0.003 m

g

/l Ib/day

<0.003 1

-2P. #-BHC.(3l9-8546)T'!

  • ~~~~~~~~ ~~X

<0.003

_I lb/day

<0.003 I

!3P.fi.BHC (31985-7)

X 003__-________

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<.0 4P. ~~~BHC(58-8?-9)~~~~~,.

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<0.003 1

lb/day

<0.003 1

(57749)

~~

~~~~~~~~X

<0.003 1

mg/I lb/day

<0.003 1

7P~~~~ 4,4'-DDT ~~~~~~X

<0.003 1

mg/I lb/day

<0.003 I

x <0.003

~~~~~~~~~~~~~~~mg/I lb/day

<0.003I (72-54-8)

- ~~

~~~~~~~~

<0__________

______03___

mg/I lb/day

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II P~~~~~~~~~~a'-Endosulfan I ~~~~~~~~~~~~~~~~~~~~~~~~~~mg/I lb/day

<0.003 (1 iS~9.7) 4 X

<0.003 12Pf.EdtzfnX

<.31 mg/i lb/day

<0.003I I.., I

. 1 1

I I

I

Vogtle Electric Generating Plant Flow Diagram Attachment to EPA Form 2C, Section lI.A NPDES Permit No. GA0026786 SOUTHERN N COMPANY

^e,ri*.Srl,lr"I~ r,,N Note
All flows are maximum.

a I" Xl a 6 Form 2C, Attachment 1 Item Il.C. Intermittent and Miscellaneous Flows Outfall Description of Flow 002/003 - Units I and 2 Cooling Tower Blowdown These points represent the blowdown from the Unit I and Unit 2 Turbine Plant Cooling Towers. Each tower recirculates river water used to cool turbine condensate via a heat exchanger. Periodic blowdown or discharge from the system is necessary to maintain the cycles of concentration in the circulating water system to prevent the build-up of solids. The estimated flow rate during a blowdown event is approximately 4,000 gpm for an approximate duration of 3-4 hours. The discharge is routed to the Blowdown Sump Mixing Box which is a final mixing chamber.

002A/003A - Units I and 2 Cooling Tower Overflow to Storm Drains These points are utilized for cooling tower basin overflow to storm drains. Overflow events occur infrequently and are generally the result of an operational problem with the system. The estimated flow rate in the event of an overflow of the basins is 2,000 gpm for an approximate duration of 1-2 hours.

004/005 - Units I and 2 Waste Water Retention Basins 006 - Sewage Treatment Plant Emergency Overflow 007/008-Units I and 2 Liquid Radwaste Systems Discharge These points represent the permanent collection basins (420,000 gallon capacity each) for plant low volume waste streams. The basins provide aeration and retention time for the wastes. Each basin has two transfer pumps which discharge the effluent to the Blowdown Sump Mixing Box. The pumping capacity will all four pumps running is rated at 2,600 gpm.

This point represents the collection point for discharge from the plant sewage treatment plant in the event of an emergency. During normal operation, discharge from the sewage treatment plant is routed to the Waste Water Retention Basins. The discharge during emergency overflow is approximately 125 gpm, and emergency overflow events occur infrequently.

These points represent wastewater generated in the Reactor Building and Auxiliary Building which may be slightly radioactive. Radiologically contaminated fluids are treated and monitored for radioactivity levels in accordance with Nuclear Regulatory Commission (NRC) regulations prior to release. The maximum flow is approximately 200 gpm (combined for both units).

Vogtle Electric Generating Plant, NPDES No. GA0026786 Page 1 of 2 Vogtle Electric Generating Plant, NPDES No. GA0026786 Page I of 2

I. I Form 2C, Attachment 1 (Continued)

Item II.C. Intermittent and Miscellaneous Flows 009 -

Nuclear Service Cooling Towers Outfall Description of Flow 009 - Nuclear Service Cooling Towers (Units I and 2) 010- Radwaste Dilution Flow 011 - Intake Screen Backwash This point represents the system which supplies cooling water for safety related equipment. Each-unit has-its own separate NSCW system which consists of two cooling towers. The discharge of each tower is routed to the Blowdown Sumnp Mixing Box. The maximum combined flow for both units is approximately 500 gpm.

This point represents an internal stream consisting of river water with no additives. The discharge is routed to the Blowdown Sump Mixing Box to provide dilution water for radwaste discharges. The maximum flow for this discharge point is approximately 46,000 gpm.

This point represents a discharge consisting only of river water with no additives. The flow is used to backwash the intake screens, and is returned directly to the Intake Canal Maximum flow for this discharge point is approximately 1,256 gpm.

Vogt_

Ecti Geeatn PnNDSN.G006 ae2o Vogtle Electric Generating Plant, NPDES No. GA0026786 Page 2 of 2

IL 6.

Requested Permit Revisions Vogtle Electric Generating Plant NPDES No. GA0026786

^

Submitted by Southern Nuclear Operating Company December 2003 S"TfHLRNAW.

CO MPAW~y

I's Vogtle Electric Generating Pla NPDES Permit Renewal Proposed Changes The following is a list of the proposed changes to the Vogtle Electric Generating Plant NPDES permit:

--1.-Change-the sampling frequency for outfalls 007 and 008 (Liquid Radwaste System Unit I and Unit 2) from once per quarter to once per six months. Past data indicates that theseedischarges-are---- --

consistently meeting limitations and that more frequent sampling is not warranted.

2. Southern Nuclear requests the addition of the following item to the Special Requirements section of the permit.

"In accordance with 40 CFR 122.41 (m)(2), the permitlee may allow any bypass to occur which does not cause effluent limitations to be exceeded, but only if it also is for essential maintenance to assure efficient operation. These bypasses are not subject to the provisions ofparagraphs (m)(3) and (m)(4) of 40 CFR 122.41 or section II.A.5 of this permit. Sampling results from a bypass of this nature shall be reported in a suitably concise format on the quarterly Operation Monitoring Report (OMR) for the period in which the bypass occurred."

3. For outfalls 002, 003, 002A, and 003A, change footnote 8 to apply to both Chromium and Zinc. This change would be consistent with other South-efWNuclear NPDES permits issued by EPD.

Vogile Electric Generting Plant, NPDES No GA0026786 Page 1 of Vogde Electric Generating Plant, NPDES No. GA0026786 Page I of I

1 4 1 v & 

Whole Effluent ToxicityxChronic Testing Summary Report Vogtle Electric Generating Plant NPDES No. GA0026786 Submitted by Southern Nuclear Operating Company December 2003 SOUTH tiN A COMPANY

CHRONIC TOXICITY TESTING REPORT SOUTHERN NUCLEAR PLANT VOGTLE EFFLUENT WAYNESBORO, GEORGIA Test Period: November 18-25, 2003 Prepared for:

Southern Nuclear Birmingham, Alabama December 2003

11 MACTEC December 3, 2003 Mr. Greg Elmore Southern Nuclear 40 Inverness Center Parkway Birmingham, AL 35242 Fax: 205-992-6108

Subject:

Chronic Toxicity Testing of Southern Nuclear Plant Vogtle Effluent, Waynesboro, Georgia, November 18-25, 2003 MACTEC Project 6308-03-0045

Dear Mr. Elmore:

MACTEC Engineering and Consulting, Inc. (MACTEC) BioTox Laboratory has completed multi-concentration chronic toxicity testing using the water flea, Ceriodaphnia dubia, and fathead minnow, Pimephales promelas, on Southern Nuclear's Plant Vogtle effluent samples collected by Southern Nuclear personnel November 16-21. 2003.

Plant Vogtle's Instream Waste Concentration (IWC, or the estimated concentration of effluent in the receiving stream during low flow conditions, 7Q10) designated for the permitted discharge of effluent into the receiving stream is 3.18% effluent. Chronic toxicity w as not exhibited to water flea survival or reproduction at -the IWC when statistically compared to the control treatment. The No Observable Effect Concentration (NOEC), or the highest concentration tested that did not exhibit chronic toxicity, wvas 25.4% effluent for water flea survival and reproduction. When the NOEC is less than the hVC, chronic toxicity is indicated in the effluent samples submitted. In -this case, the water flea NOECs are not less than the IWC; therefore, chronic toxicity was not indicated to the wvater flea in Plant Vogtle's effluent.

Chronic toxicity was not exhibited to fathead minnow survival or growth at the IWC when.

statistically compared to the control treatment. The NOEC, therefore, was 100% effluent for fathead minnow survival and growth. The NOECs for fathead minnow survival and growth are not less than the INVC; therefore, chronic toxicity wvas not indicated to the fathead minnow in Plant Vogtle effluent.

Results are summarized in the accompanying report (55 total pages).

All test results contained herein comply with the requirements of the National Environmental Laboratory Accreditation Conference (NELAC). A summary of test conditions, as well as chemical and physical data, are located in Appendix A (10 total pages). Sample collection locations, dates, times, and temperatures.

are located in the attached chain of custody documents in Appendix B (4 total pages). Test organism source data are located in Appendix C (4 total pages). Raw laboratory data and statistical analyses results are located in Appendix D (24 total pages).

1 of 8 MACTEC Engineering and Consulting 3200 Town Point Drive NW, Suite 100

  • Kennesaw, GA 30144 770.421-3400
  • Fax: 770.421-3486
  • oChronic Toxicity Testing ofSoutlher Nuclear's Plant Vogile Efflueht 8/5-12/03 December 3. 2003 3

MACTEC Project No. 6308-03-0045.01 5

If there are any questions, please do not hesitate to contact Andrew S. Peiken at (770) 421-7027.

Sincerely, MACTEC ENGINEERING AND CONSULTING, INC.

Andrew S. Peiken, C.E.

Margaret. Tanner BioTox Laboratory Manager Senior Environmental Engineer

Attachment:

Data Report 2of8

Chronic Toxicity Testing of Southern Nuclear's Plant Vogile Effluent 8/5-12/0O I fACTEC Project No. 6308-03-0045.01 TEST

SUMMARY

December 3, 2003 I. Client Facility Tested:

NPDES Number:

Samples Tested:

Southern Nuclear Plant Vogde River Road Waynesboro, Georgia 30830 GA0026786 Plant Vogtle Effluent (MACTEC LAB ID # 03-0160-01, 03-0162-01, 09-0164-01) 1I. Laboratory Accreditation Laboratory:

BioTox Laboratory Mactec Engineering and Consulting, Inc.

3200 Town Point Drive N.W., Suite 100 Kennesaw, Georgia 30144 Accreditor:

State of Florida, Deptartment. of Health Bureau of Laboratories Accreditation ID:

Lab ID. E87477 Category:

Non-Potable Water - Whole Effluent Toxicity Effective:

July 1, 2003 through June 30, 2004 III. Tests Conducted Test:

Test Dates(Times):

Source/Age of Organisms:

Test Concentrations:

Water Flea, Ceriodaphnia dubia Chronic Definitive Survival and Reproduction Test EPA-82 1 -R-02-013, 2002 Fathead minnow, Pimephales promelas Chronic Definitive Larval Survival and Growth Test EPA-82 1 -R-02-013, 2002 Water Flea: November 18 (1440) - 24 (1245), 2003 Fathead Minnow: November 18 (1500) - 25 (1645), 2003 Water Flea: In-house cultures, ID# CERB01110703 /< 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> Fathead minnow: C-K, ID# CS906111803 / < 48 hours5.555556e-4 days <br />0.0133 hours <br />7.936508e-5 weeks <br />1.8264e-5 months <br /> Control, 1.59%, 3.18%, 6.36%, 12.72%, and 25.4% effluent 3of8

i i

Chronic Toxicity Testing ofSoutheni Nuclear s Plant Vogtle Effluent 8/5-12/03 AfAC7EC Project No. 6308-03-0045.01 December 3. 2003 Control Water:

Deviation From Test Statistical Analyses:

Water Flea: DMW, Diluted Mineral Water, 20-percent Perrier.

Fathead minnow: LWC, moderately hard reconstituted fresh water.

None Toxstat 3.5 (Gulley, 1996)

IV. Results Survival and Reproduction Data Effluent. November 18-24. 2003.

for Water Fleas Exposed for Six Daysd to Plant Vogtle

% Total Effluent Survival Reproductions Laboratory Control' 100 18.2 1.59 90 19.2 3.18 100 19.4 6.36 100 20.3 12.72 100 22.9 25.44 100 21.4 NOEC'(% Effluent)

-25.44 25.44 Prepared by. ASP I 3.'03 Checked bvy SEC 12/3/03

'Mean number of young (neonates) per original number of female water fleas, excluding those accidentally killed.

b DMW = Diluted Mineral Water, Moderately Hard Synthetic Freshwater

'NOEC = No Observable Effect Concentration See Section V. Summary for explanation.

d Test ends when 60% of the surviving controls have had third brood.

Survival and Growth Data for Fathead Minnows Efinient-Nnvember 1 R-25. 2003.

Exposed for Seven Days to Plant Vogtle

% Total Effluent l

Survival. -

Growth' Laboratory Control' 100 0.462 1.59 100 0.509 3.18 100 0.502 6.36 100 0.446 12.72 100 0.415 25.44 90 1

0.442 NOEC'(% Efuent) l 25.44 25.44 Prepmared by ASP 12/3/03 Checked by. SEC 12/3/03

' Mean dry weight per original number of fish used at test start, excluding those that were accidentally killed or missing.

b LWC = Laboratory Water Control, Moderately Hard Synthetic Freshwater c NOEC = No Observable Effect Concentration. See Section V. Sununary for explanation.

4of8

Chronic Toxicity Testing of Southern Nuclear's Plant Vogile Effluent 865-12/03 December 3. 2003 MACTEC Project No. 6308-03-0045.01 V. Summary Plant Vogtle's Instream Waste Concentration (IWC, or the estimated concentration of effluent in the receiving stream during low flow conditions, 7Q10) designated for the permitted discharge of effluent into the receiving stream is 3.18% effluent. Chronic toxicity was not exhibited to water flea survival or reproduction at the IWC when statistically compared to the control treatment (see Figure 1). The No Observable Effect Concentration (NOEC), or the highest concentration tested that did not exhibit chronic toxicity, was 25.4% effluent for water flea survival and reproduction. When the NOEC is less than the IWC, chronic toxicity is indicated in the effluent samples submitted. In this case, the water flea NOECs are not less than the IWC; therefore, chronic toxicity was not indicated to the water flea in Plant Vogtle's effluent.

Chronic toxicity was not exhibited to fathead minnow survival or growth at the IWC when statistically compared to the control treatment (see Figure 2). The NOEC, therefore, was 100%

effluent for fathead minnow survival and growth. The NOECs for fathead minnow survival and growth are not less than the IVC; therefore, chronic toxicity was not indicated to the fathead minnow in Plant Vogtle effluent.

VU. Quality Assurance Ceriodaphnia dubia, NaCI Reference Toxicant, RT#122CCD, 10/21/03 Survival:

IC 25 = 2,333 mg/L NaCl (ACCEPTABLE)

IC 25 Range of Acceptability = 900 to 3,460 mg/L NaCI Reproduction:

IC 25 = 823 mg/L NaCI (ACCEPTABLE)

IC 25 Range of Acceptability = 440 to 1,560 mg/L NaCI Pimephales promelas, NaCI Reference Toxicant, RT# 147CPP, 10/21/03 Survival:

IC25 = 3,182 mg/L NaCl (ACCEPTABLE)

IC25 Range of Acceptability = 1,400 to 5,800 mg/L NaCI Growth:

IC25 = 2,135 mg/L NaCI (ACCEPTABLE)

IC2 5 Range of Acceptability = 1,270 to 4,150 mg/L NaCI 5of8

Chronic Toxicity Testing ofSouthern Nuclear s Plant Vogile Effluent 8/5-12/03 AMCTEC Project No. 6308-03-004S.01 December 3. 2003 i

Acute Chronic Chronic GLOSSARY AND ABBREVIATIONS Involving a stimulus severe enough to rapidly induce a response; in toxicit-tests, a response observed in 96 hours0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br /> or less typically is considered acute.

Involving a stimulus that lingers or continues for a relatively long period of time, often one-tenth of the life span or more. A chronic effect can be lethality, growth, reduced reproduction, etc.

A numeric value representing the geometric mean of the NOEC (No Observed Effect Concentration) and the LOEC (Lowest Observed Effect Concentration) by chronic toxicity testing. The chronic value is an estimate of the toxicant concentration that wvill be the actual no effect concentration based on the chronic effect tested.

Critical Value DMW EC Ft-c Graphical Method Minimum numeric value for a toxicity test endpoint (i.e., survival, growth, or reproduction) below which a given test result wvill be statistically significantly different from the control value.

Diluted Mineral Water Effective Concentration, a point estimate of the toxicant concentration that would cause an adverse response such as death, immobilization, or serious incapacitation.

Foot candles - a measure of light intensity Log concentration versus percent mortality method.

Toxicity test data are plotted on 2-cycle semi-log graph paper. The logarithmic axis Cv axis) is used for percent effluent concentration, and the linear axis (x axis) is used for percent mortality. The graph provides a reasonably accurate estimate of the LC50, but does not provide a confidence interval.

IC LC LC50 LCL LOEC LWC Inhibition Concentration, a point estimate of the toxicant concentration that would cause a given percent reduction in a biological measurement such as fecundity or growth.

Lethal Concentration, identical to EC when the observed response is death.

The toxicant concentration that is lethal to 50 percent of exposed organisms at a specific time of observation.

Lower 95-percent Confidence Limit Lowest-Observed-Effect-Concentration, the lowest concentration of toxicant to which organisms are exposed that causes adverse effects.

Lab Water Control, moderately hard synthetic freshwater prepared from MILLIPORE MILLI-QR water and reagent grade chemicals.

6of8

Chronic Toxicity Testing of Southem ANuclears Plant Vogde Effluent 8,5-12/03 M4CTEC Project No. 6308-03-0045.01 December 3. 2003 NOEC No-Observed-Effect-Concentration, the highest concentration of toxicant to which organisms are exposed that causes no observable adverse effects.

Probit Analyses Probit Analysis consists of a group of statistical methods used to analyze data from concentration-response experiments, and provides an estimate of the LC5o and the precision of this estimate.

In Probit Analysis, the percentages of affected organisms are converted to Probits (probability units), and the effluent concentrations are converted to logarithms.

The relationship between the Probits and the logarithmic values of the concentrations is approximately linear.

A Probit regression line drawn through the data points is used to estimate the LC50 and its precision estimate. To use Probit Analysis, at least two partial mortalities must be obtained in the toxicity test.

RWC Receiving Water Control UCL Upper 95-percent Confidence Limit pE/m 2/s Micro-ergs per square meter per second - a measure of light intensity 7 of 8

Chronic Toxiciry Testing of Southern Auclear's Plant Vogile Effluent 815-12f03 December 3, 2003 MACTEC Project No. 6308-03-0045-02 REFERENCES Gulley, D.D., and WEST, Inc.

1996.

TOXSTAT version 3.5.

Fish Physiology and Toxicology Laboratory.

Department of Zoology and Physiology.

University of Wyoming.

Laramnie, Wyoniing.

U.S. Environmental Protection Agency.

1979.

Methods for Chemical Analysis of Water and Wastes.

Environmental Monitoring Laboratory, U.S. Environmental Protection Agency, Cincinnati, OH.

EPA 600/4-79-020.

U.S. Environmental Protection Agency.

1999. Errata for Effluent and Receiving Water Toxicity Testing Manuals Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Orgasnisms; Shon-Tcrm Methods for Estimatig the Chronic Toxicity of Effluent and Receiving Waters to Frcshwater Orgarisms; and Short-Term Medtods for Esntiating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Orgaaisms.

U.S. Environmental Protection Agency, Office of Research and Development, Duluth, MN. EPAI600/R-98/182.

U.S. Environmental Protection Agency.

2002. Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms. Fourth Edition.

Office of Water (4303T), U.S. Environmental Protection Agency, Washington, DC. EPA-821-R-02-0 13.

8 of 8

FIGURES Figure 1. Southern Nuclear Plant Vogtle Effluent Water Flea Chronic Toxicity Test 11/18-24/03 C')

100 A 0

0

  • 28

--24 80 70 t 20 60 Level of Statistical Significance for Survival 16

50__,

Level of Statistical Significance for Reproduction 12 40 -

30 8

20 4

10 0

IIIIIIII0 0.00 1.59 3.18 4.77 6.36 7.95 9.54 11.13 12.72 14.31 15.90 17.49 19.08 20.67 22.26 23.85 25.44 Effluent Concentration (%) Survival Reproduction l 00 0

r-I 0

.o c o 0 o) c m

I I

m m

m m

m

-1 11

=

Figure 2. Southern Nuclear Plant Vogtle Effluent Fathead Minnow Chronic Toxicity Test 11/18-25/03 100 4 90 -

80 I-p I

I I

70 -

60 1I "I

I-W 50 0.60 0.55

7ev'e--iof Fi-atOt-icai7igrTic-a7 fir-su-tviva7 0.50 0.45 0.40 7e7lof diaFis Fic-al !7gWicanje foGro7h 0.35 0.30 0.25 0.20 0.15 0.10 0.05 i

0.00 9.54 11.13 12.72 14.31 15.90 17.49 19.08 20.67 22.26 23.85 25.44 Effluent Concentration 0

E 3:

0I-0 40 4 30 20I 10 --

0 i

i i

i i

0.00 1.59 3.18 4.77 6.36 7.95 I

. 0 Survival

-*-Growth

APPENDICES

APPENDIX A Chemical and Physical Data

-M-Initial Chemical Characterization of Plant Vogtle Efuent, LWC, and DMW Used in Short-term Chronic Toxicity Tests, November 18-25, 2003.

Parameter 100% Effluent Lab Water Control' Diluted Mineral Waterb Sampic date 11/16-17/03 11/18-19/03 11/20-21/03 11/18/03 11/18/03 Datc received 11/18/03 11/20/03 11/22/03 11/18/03 11/18/03 Temperature upon receipt (°C) 1.5 2.0 1.0 25.0 25.0 Dissolved Oxygen (mg/L) 8.94 8.92 8.48 7.40 7.74 pH 7.09 8.79 7.31 6.95 6.96 Total Alkalinity as CaCO3 (mg/L) 97.5 167.5 182.5 75.0 77.5 Total Hardness as CaCO3 (mg/L) 56.0 96.0 98.0 92.0 82.0 Conductivity @250C (ttmhos/cm) 358 646 577 327 163 Residual chlorine (mg/L)'

0.07 0.09 0.07

<0.01

<0.01 Ammonia-nitrogen NH3-N (mg/L)'

0.18 0.20 0.41

<0.01

<0.01 Unless othcn ise noted, all chemical analsses determined according to EPA 600/4-79-020.

LWC. moderately hard reconstituted fresh watcr b DMW, diluted mineral %water, moderately hard fresh %water

¢ Determined bv Hlach Spectrophotometric Test Kit Prepared by ASP 12/3/03 Checked by: SEC 12/13/03

Summary of Toxicity Test Conditions for the Water Flea, Ceriodaphnia dubia, Survival and Reproduction Test.

1.

Test type:

2.

Temperature (0C):

3.

Light quality:

4.

Light intensity:

5.

Photoperiod:

6.

Test chamber size:

7.

Test solution volume:

8.

Renewal of test concentrations:

9.

Age of test organisms:

10.

No. neonates per test chamber:

11.

No. replicate chambers per concentration:

12.

No. neonates per concentration:

13.

Feeding regime:

14.

Aeration:

15.

Dilution water:

16.

Effluent concentrations:

17.

Dilution factor:

18.

Test duration:

19.

End points:

20.

Test acceptability:

Static renewal definitive 23.5-26.0 OC Ambient laboratory illumination 10-20 iE/m2/s, or 50-100 ft-c 16-hr. light, 8-hr. darkness 30mL 15 mL/replicate Daily Newly hatched neonates < 24-hr. old 1

10 10

  • Fed 0. I mL each of YCT and algae suspension per test chamber daily None Moderately hard synthetic water is prepared using MILLIPORE MILLI-QR and Perrier.

Control, 1.59%, 3.18%, 6.36%, 12.72%,

and 25.4% effluent 0.5 6 days, 60% of control females had 3 broods Survival and reproduction 80% or greater survival in controls; Average of 15 or more young/surviving female in the control solutions. At least 60% of surviving females in controls should have produced their third brood.

Samples are collected daily, and used within 36-hr. of the time they are removed from the sampling device 1.0 L per day

21.

Sampling requirement:

22.

Sample volume required:

Summary of Toxicity Test Conditions for the Fathead Minnow, Pimephales promelas, Larval Survival and Growth Test.

1.

Test type:

2.

Temperature (0C):

3.

Light quality:

4.

Light intensity:

5.

Photoperiod:

6.

Test chamber size:

7.

Test solution volume:

8.

Renewal of test concentrations:

9.

Age of test organisms:

10.

No. larvae per test chamber:

11.

No. replicate chambers per concentration:

12.

No. larvae per concentration:

13.

Feeding regime:

14.

Cleaning:

15.

Aeration:

16.

Dilution water:

17.

Effluent concentrations:

18.

Dilution factor:

19.

Test duration:

20.

End points:

21.

Test acceptability:

Static renewal definitive 24.0-26.0OC Ambient laboratory illumination 10-20 ptE/m 2Is, or 50-100 fl-c 16 hrs. light, 8 hrs. darkness 400mL 250 mL/replicate Daily Newly hatched larvae < 24-hr. old 10 4

40 Fed approximately 0.1 to 0.3 mL newly hatched (less than 24-hr. old) brine shrimp nauplii three times daily. Larvae are not fed during the final 12-hr. of the test.

Siphoned daily, immediately before test solution renewal None Moderately hard synthetic water prepared using MILLIPORE MILL1-QR and reagent grade chemicals Control, 1.59%, 3.18%, 6.36%, 12.72%,

and 25.4% effluent 0.5 7 days Survival and growth (dry weight) 80% or greater survival in controls; Average dry weight of surviving controls equals or exceeds 0.25 mg.

A minimum of three samples are collected during testing 4.0 L per day a

22.

Sampling requirement:

23.

Sample volume required:

MACTEC ENGINEERING AND CONSULTING, INC.

BIOTOX LABORATORY DAILY WATER QUALITY DATA (STANDARD EPA CHRONIC TOXCITY TEST)

CLIENT: Southern Nuclear SAMPLE: Plant Voutle Effluent LOCATION: Wavnesboro. GA NPDES NO.: G6t 2 ( 7 f4 TEST TYPE: Cerio Definitive AERATION REQUIRED: N TEST CHAMBER SIZE: 30 ml TEST SOLUTION VOL.: IS en DILUTION/CONTROL ID: DMWO111703 ORGANISM ID: CERBOI 110703 FOOD TYPE: YCS/Selenastnum DATE/I1ME ORGANISMS FED: I X Daily

.;ON I a IfI I

FINAL AMAIAL~~~

FINIAAL j1 j J.V

1.

-. 6 JN111AL O

I.'

.. p.

7.I Cl

_O F~.

INI AL FINAL C-S-1

'1.

'.0'-I 3h M

=

0. - 6 7/-

77 7/-7'

/7 FINAL AK 77 I>r

~7-L

<7/

'f77/7 FINAL

-7 L40

-7.

0.

&-).

-7 6 Iii IMAALAL

  • 7 i 7bw-.

7

-I-qo

-J-f5 NAL_

-,?'

4

.7 C%

S7. I 17<

3IR ~

~

.777/777/</7777 9/777/m/

//

176 of/77/00/0/777777/07/77

/97/777

/

77 RC.

//7/7/9////n/7/

//77777/7 D

C).

717//77N1/7/

/

77T 1/7C>

  1. ///7

-//7777/ /

FIN_.~L'%

A~iL.

-'I

'.r.+.7'o j.u.

N LAL FINAL.

v.

I

-7. 2.J I

0- L 1-I 1) QI IA

.W

~

"T

-7.3 I

I I

1 4

I. -

I I-I

~~I

-GA 1

.C I

I I

- ~ ~ ~ ~ ~

4 I.

_I I 1-TIA Ii I

't41

  • NLAIAL FINAL.-

T -

I OA Ko I

"I `19 I

%~L

-I I

I I

A.LKAUNI 1 iUil q7j m jvtr1AhOlcrz I IAJle NEONDuclivill lo.I M3t1-II.A7%pn______

I 00% DO_____-?

Iu n9pn 1.3 I00%DO____

I UUVQ.

4 07

'.twmg'M uu-.

C.,g-1 00% Do_..iqj

MACTEC ENGINEERING AND CONSULTING, INC.

BIOTOX LABORATORY DAILY WATER QUALITY DATA (STANDARD EPA OIRONIC TOXICITY TEST)

I CLIENT: Southern Nuclear SAMPLE:

Plant Voztle Effluent I LOCATION: Wavoesboro. GA NPDES NO.: CIA 17,i-'

TEST TYPE: FHM Definitive AERATION REQUIRED: N TEST CHAMBER SIZE: 400 ml TEST SOLUTION VOL: 250 ml DILUTION/CONTROL ID: LWCO4 111703 ORGANISM ID: CS906111803 FOOD TYPE: artemia DATE/TIME ORGANISMS FED: 2 X Dailv IpAiAAhALI

. I I oiliffl ji I Al.liq -$I I

ollo Aio I

  • .\\t
a. A,4 t

I I% I A

16'C.3 lf hill-4 aM I

LU.%L i'. I l

i I

,uN i ROL:

.L TV li

~~.....

-I _ __

FNk I LAL FINAL tI A4b 1

Z,-;

I 1

-7.9, 1

7.Z S

I

-7Q0 I

'7.t I

b.7yr 1

1-2'-

1

-.72 1

7.ac I

-t.,

I US.-l 4

W.2.'

I lik'

~~~.UJ~~~~~~~~iJ~~~~~~AJJ~~~~~~~~h~~~~...

-..~~~~~~~~~~~~~~~~~4iO

.m.

LJ.V.

l~iNLAL FINAL

-1 6'.4

-i.

6.1 1

-7.'7 J

-7.33 I

4 -'----"

4 -,.---

I

+

.

4 4 ------

-, *U57 K1) 7 C 1'In C

I (s. ir I

r-w.-

4 

I ------9 4

4 4

pH-Ii iu LAL FINAL Ic" 'OS

-1.11 I

7.97 1 -t I

(i.1L.

I 9

I-4-"--.'-

4 1.b1 L

A\\iALiNhi i W/0 U%

7 5 HAKUNESS

-T1,-.

5 L uONh)UClIvldl m'.

S IhC mg/i Ioh.

0 e7

.ss ms, a 1uu,.

O.18-q '

a C.

7 1.S 17-93

..0 'i I

7. ZL

(-41, USI/c^

S'7 7 -,' I&-.

o C"

..' 7 100% DOH1 I00/ pHL

-00 100%1DO 1-nD loo/- PH_:3

1001/0 DO

-ir

MACTEC ENGINEERING AND CONSULTING BIOTOX LABORATORY TITRIMETRIC BENCH SHEET PARAMETER: HARDNESS METHOD

REFERENCE:

EPA 130.2 I REAGENT

REFERENCES:

ANALYST:

$ CC

1) BUFFER:

429 STANDARD

REFERENCE:

BKII p. 142

2) CALMAGITE:

228 CURVE

REFERENCE:

HRD061603

3) 0.020 N DISODIUM EDTA:

478 CLIENT:

Southern Nuclear SAMPLE LAB DATE'

SAEPLE: V B1T V~iNAL VTOTAL STD BLK CONC SAMPLE VOLUME (n)

L)

(mL)

CORR CORR (mgfL)

ID NO.,

-+mg/l +1-F I

DMW 11/18/03 25/50 0

J 0

_- L l.

LWC 11/18/03 25/50 0

0 DI Blank 11/18/03 25/50 0

d 0c.

I 50 mng/L Std.

11/18/03 25/SO 0

a.55 0

100% Vogte Eff.

03-cG to-el 11/18/03 25/50 0

I 0

100% Vogtle Eff.

O, ol L * "

11/20/03 25/50 0

C) V jJW 0

_4 100% Vogtle Eff.

t,3-Cib,1-Ce 11/22/03 25/50 0

0 I

_____(

._CO I

I _

1__

I fVtotal + (BLANK CORR + STD CORR)J x 50,000 x Ntit rant Mg/L HARDNESS =

SAMPLE VOLUME REV'IEWED BY!

O DATE: igi/31

MACTEC ENGINEERING AND CONSULTING BIOTOX LABORATORY SPECTROPHOTOMETRIC BENCH SHEET

~~~

  • ~~~.*.

1'-FMTE~t CH 'OR E

iE.E GT : 30Ji

~*.

. PARAMCTE~

E 5.,.s S..:.f M;ETHOD: HACH PROCEDURE'

.x'fS S

INSTRUMENT ID: HACH DORI2SOD

.:..~:.:HA ANALYST:

CURVE

REFERENCE:

CL2061603 STANDARD

REFERENCE:

461 REAGENT

REFERENCES:

CLIENT: Southem Nuclear 1 DPDAccuVac. #474 LWDATE:

11/18/03

~~~~~~~~~~~~~~~...

....... }

DIBlank 11118/03 9i7e-Y2 0

0.590 mg/L Std.

11/18/03 0ft

(

0

'S.

100% VogtleEff.

63 -

i bi - al 11/18/03 T8.

0 b'7 0

100% VogtleEff.

0 3 -' k, Z -it 11/20103 gs-L o-o9 q 0

o I l00% Vogtle Eff.

2-&oi-

-ok 11/22/03 S845 o-c73 0

MgtL CHLORINE =[

(MgfL CURVE x CELL CORR) + (STANDARD + BLANK CORR) I REVEIWED BY: QO;P DATE: _________

MACTEC ENGINEERING AND CONSULTING BIOTOX LABORATORY SPECTROPHOTOMETRIC BENCH SHEET PARAMETER: AMMONIA WAVELENGTH: 425 NM METHOD: HACH "PROCEDURES 8038 INSTRUMENT ID.HACH DR/2500 ANALYST:

CURVE

REFERENCE:

NH3051503 STANDARD

REFERENCE:

Bk II, pg 148 REAGENT

REFERENCES:

CLIENT: Southern Nuclear I) NESSLER RGT:

  1. 424
2) MINERAL STABILIZER: 441
3) PVA: 440 SAMPLE: D SAM DAE

/ T I

,MG CELL:

STD/BLK FINAL

-1D NO.

CURVE.
:

.. CORR CO..

CONC

.FACTOR:

(MG/L)

DMW 11/18/03 7 1.(

0 c

LWC 11/18/03 l&1o teai 0

1.5 mg/L Std.

11/18/03 7,33 I-(

0 I.

DI Blank 11/18/03 7 &,-,

0 o T

100%Vogtle o33-c6i-oi 11/18/03 (7 3.6 0- I_

0 0 100%Vogtle 073-oz62-O' 11/20/03 l

o o

0

_____3 100% Vogtle o3-o b6,-o 11/21/03 1

-i7 0

42Ot0 Mg/L AMMONIA = [(Mg/L CURVE x CELL CORR) + (STANDARD + BLANK CORR)]

REVIEWED BY:

aV9 DATE:

n/pg -J

MACTEC ENGINEERING AND CONSULTING BIOTOX LABORATORY TITRIMETRIC BENCH SHEET PARAMETER: ALKALINITY

METHOD

REFERENCE:

EPA 310.1 D REAGENT

REFERENCES:

ANALYST:

5 P (I

1) 0.02 N H2SO4:

454 STANDARD

REFERENCE:

BKII p. 141 CURVE

REFERENCE:

ALK061603 CLIENT;:

5CJ4. o

_____e SAMPLE ID LAB ID DATE SAMPLE..: V VINAL VTOTAL STD BLK CONC

.NO.

VOLUME,: (mL: (mL)

(niL)

CORR CORR (mg/L)

N o._

(+1-) :

(+1-)

DMW 11/18/03 20/40 0

1

. 6 0

T

'77.r LWC 11/18/03 20/40 0

I Js I-SIX 0

._._1So 12D mg/L Std.

11/18/03 20/40 0

Z-tex 2*r-.

0

___3o DI Blank 11/18/03 20/40 0

O '

C. Sz 0

Z O *,

100%Vogtle v3-Oi6 c e 11/18/03 20/40 0

0

=

=

9.

I100% Vogtle o;

  • O6/-

I 11/20/03 20/40 0

Y 0

I z_

  • S 100% Vogtle c;i-c/

C-11/22/03 20/40 0

0

__=

I

=______

IVtotal + (BLANK CORR + STD CORR)] x 50,000 x Ntitrant Mg/L ALKALINITY =

SAMPLE VOLUME REVIEWED BY: q44 DATE: I-I U'3

APPENDIX B Chain of Custody Records

w,A N° 05129 MACTEC ENGINEERING AND CONSULTING BIOTOX LABORATORY 3200 Town Point Drive NW, Suite 100 Kennesaw, GA 30144 (770) 421-3312 - FAX (770) 421-3314 CLIENT INFORMATION NAME OF FACILITY: S N C( -

tor STREET ADDRESS ?O. 'tSi.

\\

(kZdC

.~ El CIlYISTATE: vi ft4&5

&1f2.

C'4 A

zPip, NPDES o (NATIONAL ORSTTE) G P _La79Cl PROJECT NAME MACTEC PROJECI Nb~t$

StIC_-

Cloru A Z11.-7 Z t SAMPLERS (SIGNATURE)

SAMPLERS INITIAl

+

SAMPLES SHIPPED VIA:

FEDEX V

UPS HAND OTHER II

.S (PRINT)

SAMPLE INFORMATION GRAB SAMPLES: DATEfTIME VOL_

TIME COMPOSITE: STARTDATE1It' f'i 3 STARTTIMEoabOe ENDDATE l-ENDTIME O1vCW VOLUME OF SUBSAMPLES UeC Ct TIME INCREMENT _kourl_ _

TOTAL VOL________

FLOW-PROPORTIONED COMPOSITE: INITIATED ENDED TOTAL VOL SET VOL OF SU8SAMPLE I VOL FLOW FIELD PARAMETERS GRAB SAMPLES: pH DO (mg/I)

TEMP CC) _

CHLORINE (resdirual. mg/L)

COMPOSITES: pH (asl grab)

V ¢ CHLORINE (residual lasil grab. mg)L) 0 O TEMP (last rab C)_

pH(composlled smrpe. C)

F I

TEMP (composld sample, C) 3' Ift o COOLANT USED:

SHIP DATE,e I-I-

o3 DATE TIME GRAB COMPOSITE MATRIX SAMPLE STATION DESCRIPTION TOTALIOF CONTAINER TYPE I NUMBER

^'

BIOTOX USE ONLY,"

(X)

(X)

CONTAINERS I OALLON 7 2UTER

_1UTER T OTHER

TEMP

)Cj

':U IELINOUISHEDo

, DATE/TIME RECEIVED BY.

DATE/TIME RELINQUISHED BY:

RECE7?BY BORAT YI DATEITIME (IN R -W 1S7IG5AURE 1

(SIGOTURE-(ARE 1________3 (ItGNATURE).

( SIGNATURE)

(SIGNATURE) l(SIGNATURE)

O 1? r.-l FOR VARIABLE VOLUME SUBSAMPLES BASED ON FLOW OR SET TIME INCREMENTS. ATTACH SAMPLE AND FLOW INFORMATION.

BIOTOXUSEONLY.

ICE PRESENT? -_iE pH SD D.

CONDUCiTPA' VIS:AL'

1

    • MATRIX WATER. W SLUDGE-SL SOIL -SO OTHER. NA Il SEDIMENT-SD I

Distributon: OrtgInal Copy Accompany Sample ro Laboratory. Yellow Copy Retained By Sampler.

F1008-A 8/03

.r

r N2 05131 MACTEC ENGINEERING AND CONSULTING BIOTOX LABORATORY 3200 Town Point Drive NW, Suite 100 Kennesaw, GA 30144 (770) 421-3312

  • FAX (770) 421-3314 CLIENT INFORMATION NAMEOFFACILRy:

S'4L( -Vc6GfL.E STREETADDRESS:O t

'60

)O' U

( Rw-F

)I CITYISTATE: %A 9I`4NQ&

o, 4GN ztp g_3 NPDES U (NATIONAL OR STATE):

A

&9 2 6 7846 PROJECT NAME c - xi0TLE-SAMPLERS (SIGNATURE)

WJ t

-8ch MACTEC PROJECT #

SAMPLERS INITIALS (PRINT)

'-I-SAMPLE INFORMATION GRAB SAMPLES: DATEITIME VOL_

TIME COMPOSITE:

STARTDATE Ikej-o3 STARTTIME_ ____

END DATE I1 C0 3

END TIME l u VOLUME OF SUBSAMPLES l

TIME INCREMENT.Lt* "6 TOTAL VOL....4oL.

FLOW-PROPORTIONED COMPOSITE: INmATED_

ENDED TOTAL VOL SrT VnO OF SDIBFSAUMPLE I V t

FOW FIELD PARAMETERS GRAB SAMPLES: pH DO (mgl)_

TEMP CC)

CHLORINE (resdkual. mgtL)

COMPOSITES: pH (last grab)

CHLORINE (residul, last grab, mgIL)

TEMP (last grab. 'C) pH (composied sample. C)

TEMP (womposlled sample. 'C)

SAMPLES SHIPPED VIA:

FEDEX N/

UPS HAND___

OTHER COOLANT USED:

SHIP DATE I \\ - let -o3 DATE TIME GRAB COMPOSITE MATRIX SAMPLE STATION DESCRIPTION TOTAL I OF CONTAINER TYPE I NUMBER11 BIOTOX USE ONLY :.

(X)

(X)

CONTAINERS 1 OALLON 2 UTER I i UTER OTHER

TEMP ('C)

LABS iwoo V.4 FlMM.

eFFLUG14iT 0

3 PELINCUISHED BY:

DATE/TIME RECEIVED BY.

OATE/TIME RELtNOrSHED BY.

lR VDB OTDRYI DATEITIME 1 nitl l rI f l

III lilltt (SIGNATURE)

SCt l

(SIGNATURE)

(SIGNATURE)

(SIGNATURE)

-F O R V A R I A B L E_

V O U M IUS M P E

( SIAA T R E O N_

( SO WN A T U R E)IM E I N R M N S A T Cq x~

N L

W N O M T O FOR VARIABLE VOLUME SUBSAMPt ES BASED ON FLOW OR SET TIME INCREMENTS. ATTACH SAMPLE AND FLOW INFORMATION ICE PRESENT?

YES I N RE 6L DO CON VISUAL WATER -W SOIL - SO SLUDGE - SL OTHER-NA D1stribuffon: OrIgInal Copy Accompany Sample To Laboratory. Yellow Copy Retalned By Sampler.

F1008-A *8103 Li SEDIMENT-SD

Cr 4TIE

& HtU-D N2 05130 MACTEC ENGINEERING AND CONSULTING BIOTOX LABORATORY 3200 Town Point Drive NW, Suite 100 Kennesaw, GA 30144 (770) 421-3312

  • FAX (770) 421-3314 CLIENT INFORMATION NAME OF FACILITY: *N C, VL6 tGL1 STREETADDRESS:

V P° OX I 56s ClTYtSTATE:

&4j4/ i'Ld 4

6A ZIP: 3c'Y33 NPDES C (NATIONAL OR STATE):

G A oa I-,72?G

.1 PROJECT NAME MACTEC PROJECT #

Vo/L6 -L-s SAMPLERS (SIGNATYRl SAMPLERS INITIAL 1;

.m.g

.f14 SAMPLES SHIPPED VIA:

FEDEX V/

UPS HANDO OTHER

.S (PRINT)

SAMPLE INFORMATION GRAB SAMPLES: DATETIME VOL_

TIME COMPOSITE: STARTDATE II_2c -e STARTTIME____

ENDDATE Ift ENDTIME Q'CO z,

VOLUME OF SUBSAMPLES

.3'g Z2I TIME INCREMENT/k TOTALVOL f

'1 FLOW-PROPORTIONED COMPOSITE: INITIATED_

ENDED TOTAL'VOL_

FIELD PARAMETERS GRAB SAMPLES: pH DO (mgt))

TEMP CC)

CHLORINE (resdual, mg/t)

COMPOSITES: pH (last grab)

CHLORINE (residual. last grab, m/gL)

TEMP (laos grab, *C) pH (composkled sample. *C) _

TEMP (conionasied seamle. *C)

COOLANT USED:

_CC_

SHIP DATE II%

-7_% -0 5_

fl cc, vt~t.. ~,r ouoon,.rI, VhJL. r b~bLtjVUtLV DATE TIME GRAB COMPOSITE MATRIX-SAMPLE STATION DESCRIPTION TOTAL I OF CONTANER TYPE I NUMBER BIOTOX USE ONLY.--:. '

(x)

(X)

CONTAINERS IGALLON l

2UITER I 1LITER l

OTHER

".TEMP'C)

,..,.LAB I

,z=

13 Li j(r o;:

tvUN~tWYQ lODATEITIME lRECEIVED BY:

DATEITIME REULNOUISHED BY:

lRE RAEDqABORATY I ATE/TIME ISIGNATURE)

I I

S S

I I

II______ I l

~~~(SIGNATURE)

(

O SIGNATURE)

(SIGNATURE) l(SIGNATURE)

-FOR VARIABLE VOLUME SUBSAMPLES BASED ON FLOW OR SET TIME INCREMENTS, ATTACH SAMPLE AND FLOW INFORMATION lsIOTox USE ONLY..,,'ts 3'>'

,^

!;

  • Xsi.,* -

s

.W I N

.'RESIDUALCL

_____VSUAL ICE PRESENT?

IN PHO COUCiI______VSA_____

I

  • 'MATRIX WATER-W SLUDGE-SIL SOIL-SO OTHER-NA Il
  • SEDIMENT-SO I

Distribution: Original Copy Accompany Sample To Laboratory. Yellow Copy Retained By Sampler.

F1008-A 8/03

APPENDIX C Test Organism Documents

MACTEC Engineering and Consulting, Inc.

BioTox Laboratory Ceriodaphnia dubia In-house Culture Source Document Brood Board ID Brood Board Date/Time Cups.

Age of Test Water Food Type No.

Date Marked Organisms Type/Temp.

(OC )

CERBO 1 10703 11/07/03 11/18/03 0900

<24-hr DMW /25.0 YCT &

Selenastrum

MACTEC Engineering and Consulting, Inc.

BioTox Laboratory Pimephales promelas In-house Culture Source Document Larvae Batch Source Hatch Date Age of Test Water Food Type ID No.

Organisms Type/Temp.

CS9 6111803 C-K 11/17/03

<48-hr LWC / 25.0 Arnemia

I.I 1C-K I

  • A S S 0 C I Al7 E S -

ENVIRONMENTAL & ENGINEERING

-CONSULTANTS I

17170 PERKINS ROAD BATON ROUGE, LA 70810 PH (225) 75S-1000 FAX (225) 755-2042 hUT p://wwwI C-LoR AouAnc ToxIcoLoGy LAWEATOR Test Organism Shipment Record Shipped to: Hai c

Project*. b 55 -4a Date Shipped:

II - 1i-03 P.O.#: ________

Species: *.

SenGdYL" Quantity:

(4C

/

Price each:

25¢/,

?Z9' jI Age/Lifestage:

c24 ag Price Batch #: 33Y/

/

/eQ3 Broodstock Source: C-K Associates, Inc.

Feeding:

a4e4rM&-'

Water Chemistry Record

___Freshwater Species Marine Species Alkalinity (mg/L):..

D Alkalinity (mglL):_

Hardness (mg/L):

94 Salinity (ppt):

Comments:,

. J I

Billing Information (This is not an invoice. You will be billed separately.)

I Organism Price:

I 8 City tax (5%)

State tax (4%)

Shipping Charge:

(if not billed directly to recipient's Fed Ex account)

Shipment Total:______

Effective February 2001, C-K Associates, Inc. will mail you an invoice for the total amount of alll purchases made within the month. If you have any questions, please feel free to contact us.

l Thank You oiJ /

reV olrcS5 test organism shipment record.doc

I I I

I I

I I

i I

I I

APPENDIX D I

Laboratory Test Data Sheets and Statistical Analyses

'I I

I I

I I

I

Fathead Minnow Effluent Test

MACTEC Engineering and Consulting, Inc.

BioTox Laboratory 7-day Fathead Minnow Larval Survival and Growth Test (EPAI421 -R-02-013, 1000.0)

Client:

Location:

Sample:

Minnow l.D. #:

Southern Nuclear Waynesboro, GA Plant Vogtle Effluent Test Location:

INC #

L03 Shelf #

CL...

CS906111803 Surviving Organisms (alive/dead)

Day 0

1 2

3 4

5 6

7 ConcentratiouiRep. #

date: ills 13 lime: j5jc, init: t date:atl lo3 time: 155f init: CvA*

date: q.j zIJ3 time: I1U

.inH: 4 date:%lz.161 lime: i St inH: 1,V date: t'l time:,, ;I init: all date: 1112) time: II, -L, ini: Q'1 date: itp.

time: I S rt '

int: ao/-

dale: (f(2i time: lb-ir init: o1 0% Survival 1.

'- 13 1I

/0O 10 1.J

' tJ LWC to' to10 I

1

.I

.a

__I I

I__

I

_ I_

.I I a

.~~~~~I 25ii I0 '.

Temp CC)

ZS 2 L.

Lt ru 2Js "S

1

) s rI 10 I I..

I-)

(J t i 1.59%

2 2

i ij la IO 10 (o

(.

I

J I

I 3o O

tj to I

(J I

(J I

'(J I

4 I

I 4 I O

I II lo I

II

t.

I tV

[Temp (C)

Z7 L t k 17 r 2S 1

jo o

jL If j_

[o j

-t j

.0 j

j 1 2 11 o

I I

(O II l3-18%

l 3

la 1

I0 fitto1 l

IC _I iJ t

(IWC)

I 4

la to 34 I

l~~~~Temp(°CCl 1

4 S-2 q 1 4 T

fS T2 l2~

1 B 2

,11

-ia.I....-I j

I 1D I

I AI IJ I C I

6.36%

l 3

11 /J Lo l

I I ~~~~4 Jo to 11 Temp CC) l ZC.

l r

-L L.

l 2sr l

(o I 0 C C

_l

_r I

1 ~ ~2 j1 o

~

(#3

  • **o 1

ie I

1 12.72%

3 11 1 1 4

13 I

Temp CC) 2V I

_L l

z I

___l z*L I

s_11

[

~~~~~I inl I

VI

_I I

I :'

71 II I

I ~~~~2 in I"

I{

1ZFIs I

25.4%

3

3 I I I

_I

_I 13 1'

13 10 j

I 4

1T

__to LO I1_ _

l l~~~~Temp (CC)

Z@

U t;

I l Z

-if lZ Z.

I

_i lZ

(

l 25 l

Sample ID:

Comments:

c03-. 11, -ot

MACTEC ENGINEERING AND CONSULTING, INC.

BIOTOX LABORATORY Laboratory weight data for larval survival and growth test Client:

Discharge:

Location:

Southern Nuclear Test Dates: 11/18-25/03 VogticEffluent o3 *'I

-01,03

°)4&

4)rganism: P.promelas. CS906111803 Wavnesboro. GA Drying Temp.:

.35 C Drying Time:

-2.r A-Analyst(s):

9

f

.~

~

~

Conc.:

Replicate No.

Weight of.

boat (gms)

Conc.:

I Q.-~75

.s z-2 2

0. 97 Z-5 LWC 3

O.q74 4 4

OA1-; 5 c,0 Conc.:

1

.97(z 2

1.59%

3 Cone..

r-97t 7 2

2

e.
  • 7 ZU.

3.18%

3 e.q w, Conc.:

I C.q1910 t, 2

0.*1/ 6Z..

6.36%,3 C

k/

4 r,.TF24 f Conc.:

I

. 46 3 2

61q9J7"3 12.720%

3 opy37 4

6a1777 ci Conc.:

I

~

y 7 2

OD.975'7 25.44%

3

.i 9

4 w.

Dryweight Total dry Original Mean dry boat and

':weight of Number weight per rep.

larvae larvae of original number (gmns)

(gms) larvac of larvae Idav (in)

C.9o y0 7

/

( a/

0- f7S3 77 V.l a fF37Y l 2

?75lcyS F

l2 0-95s

/

// 7/

7/

ODfo.

./

UIINZO J/

Mean dry weight per conc. original number larvae Oven Times

  • in Out Dot*:

Dow.:

Is(

MACTEC ENGINEERING AND CONSULTING, INC.

BIOTOX LABORATORY Laboratory weight data for larval survival and growth test Client:

Discharge:

Location:

Southern Nuclear Vogtle Effluent Wavnesboro. Ga Test Dates:

1/1 8-25/03 Organism: P. promelas. CS906111803 Sample ID: 03-0161-01. 03-0162-01.03.0164-01 Drying Temp.: 59 C Drying Time: 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> Analyst(s): ASPIMRF Weight of:: Dry weight:

Total dry Original Mean dry Mean dry Oven Times Conc.:

Replicate boat

. boat and weight of Number.

weight per rep.

weight per In Out No.

(gins) larvae larvae:,

of original number conc., original (gmns)

(gins) larvae of larvae number larvae Date:

Date:

Jdav 01 (mg)

(me) 12s2z03 I1J26-2DO3 Conc.:

1 0.97592 0.98046 0.00454 1 0 0.454 1645 1700 2

0.97254 0.97583 0.00329 10 0.329 1645 1700 LWC 3

0.97468 0.98008 0.00540 1 0 0.540 0.462 1645 1700 4

0.97850 0.98374 0.00524 10 0.524 1645 1700 Conc.:

1 0.97609 0.98104 0.00495 10 0.495 1645 1700 2

0.97705 0.98154 0.00449 10 0.449 1645 1700 1.59%

3 0.96851 0.97427 0.00576 10 0.576 0.509 1645 1700 4

0.97844 0.98358 0.00514 10 0.514 1645 1700 Cone.:

1 0.96737 0.97208 0.00471 1 0 OA71 1645 1700 2

0.97263 0.97791 0.00528 10 0.528 1645 1700 3.18%

3 0.96489 0.96963 0.00474 10 0.474 0.502 1645 1700 4

0.97193 0.97726 0.00533 10 0.533 1645 1700 Cone.:

1 0.97096 0.97559 0.00463 1 0 0.463 1645 1700 2

0.97162 0.97590 0.00428 1 0 0.428 1645 1700 6.36%

3 0.96710 0.97162 0.00452 1 0 0.452 0.446 1645 1700 4

0.98225 0.98667 0.00442 10 OA42 1645 1700 Cone.:

1 0.96633 0.97036 0.00403 10 0.403 1645 1700 2

0.96231 0.96676 0.00445 10 0.445 1645 1700 12.72%

3 0.97832 0.98239 0.00407 10 0.407 OA15 1645 1700 4

0.97729 0.98135 0.00406 10 OA06 1645 1700 Cone.:

1 0.98575 0.98967 0.00392 10 0.392 1645 1700 2

0.97570 0.97999 0.00429 10 0.429 1645 1700 25.44%

3 0.97043 0.97505 0.00462 1 0 0.462 0.442 1645 1700 4

0.96766 0.97251 0.00485 10 0.485 1645 1700 Prepaired by Reviewed by (ID g1z1Z6

< 4d i// 2 V

A

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Survive File:

111803

.PPS Transform:

ARC SINE(SQUARE ROOT(Y))

Number of Groups:

6 GRP IDENTIFICATION REP VALUE TRANS VALUE 1

0 LWC 1

1.0000 1.4120 1

0 LWC 2

1.0000 1.4120 1

0 LWC 3

1.0000 1.4120 1

0 LWC 4

1.0000 1.4120 2

1.59 t 1

1.0000 1.4120 2

1.59 %

2 1.0000 1.4120 2

1.59 t 3

1.0000 1.4120 2'

1.59 01 4

1.0000 1.4120 3

3.18 c 1

1.0000 1.4120 3

3.18 %

2 1.0000 1.4120 3

3.18 %

3 1.0000 1.4120 3

3.18 %

4 1.0000 1.4120 4

6.36 %

1 1.0000 1.4120 4

6.36 t 2

1.0000 1.4120 4

6.36 %

3 1.0000 1.4120 4

6.36 %

4 1.0000 1.4120 5

12.72 1

1.0000 1.4120 5

12.72 t 2

1.0000 1.4120 5

12.72 %

3 1.0000 1.4120 5

12.72 !

4 1.0000 1.4120 6

25.4 1

0.7000 0.9912 6

25.4 i 2

0.9000 1.2490 6

25.4 '

3 1.0000 1.4120 6

25.4 6 4

1.0000 1.4120

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Survive I

File:

111803

.PPS Transform:

ARC SINE(SQUARE ROOT(Y))

Summary Statistics on Transformed Data TABLE 1 of 2 GRP IDENTIFICATION N

MIN MAX MEAN 1

0 LWC 4

1.4120 1.4120 1.4120 I.

2 1.59 %

4 1.4120 1.4120 1.4120 3

3.18 '

4 1.4120 1.4120 1.4120 4

6.36 16 4

1.4120 1.4120 1.4120 5

12.72 %

4 1.4120 1.4120 1.4120 6

25.4 %

4 0.9912 1.4120 1.2661 B

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Survive File:

111803

.PPS Transform:

ARC SINE(SQUARE ROOT(Y))

j Summary Statistics on Transformed Data TABLE 2 of 2 GRP IDENTIFICATION VARIANCE SD SEM C.V. %

1 0 LWC 0.0000 0.0000 0.0000 0.0000 2

1.59 %

0.0000 0.0000 0.0000 0.0000 o 3 3.18 '

0.0000 0.0000 0.0000 0.0000 I

4 6.36 t 0.0000 0.0000 0.0000 0.0000 5

12.72 '0.0000

-0.0000 0.0000 0.0000 6

25.4 %

0.0395 0.1987 0.0994 15.6960

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Survive File:

111803

.PPS Transform:

ARC SINE(SQUARE ROOT(Y))

Shapiro -

Wilk's Test for Normality D =

0.1185 W =

0.4845 Critical W

= 0.8840 (alpha = 0.01, N = 24)

W

= 0.9160 (alpha = 0.05, N = 24)

Data FAIL normality test (alpha = 0.01). Try another transformation.

Warning The first three homogeneity tests are sensitive to non-normality and should not be performed with this data as is.

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Survive File:

111803

.PPS Transform:

ARC SINE(SQUARE ROOT(Y))

Hartley's Test for Homogeneity of Variance Bartlett's Test for Homogeneity of Variance These two tests can not be performed because at least one group has zero variance.

Data FAIL to meet homogeneity of variance assumption.

Additional transformations are useless.

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Survive File:

111803

.PPS Transform:

ARC SINE(SQUARE ROOT(Y))

Steel's Many-One Rank Test Ho: Control<Treatment TRANSFORMED RANK CRIT.

SIG' GROUP IDENTIFICATION MEAN SUM VALUE DF 0.05 \\

1 0 LWC 1.4120 2

1.59 %-0 1.4120 18.00 10.00 4.0 3

3.18 q 1.4120 18.00 10.00 4.0 4

6.36 %

1.4120 18.00 10.00 4.0 5

12.72 %

1.4120 18.00 10.00 4.00 6

25.4 '

1.2661 14.00 10.00 4.00 Critical values are 1 tailed ( k = 5 )

, N D/

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Growth File:

111803

.PPG Transform:

NO TRANSFORMATION Number of Groups:

6 GRP IDENTIFICATION REP VALUE 1

1 1

1 2

2 2

2 3.-

3 3

3 4

4 4

4 5

5 5

5 6

6 6

6 0 LWC 0 LWC 0 LWC 0 LWC 1.59 %

1.59 %

1.59 9 1.59 '

3.18 %

3.18 %

3.18 %

3.18 !

6.36 6 6.36 '

6.36 a 6.36 '

12.72 6

12.72 t 12.72 %

12.72 t 25.4 6 25.4 %

25.4 '

25.4 6 0.4540 0.3290 0.5400 0.5240 0.4950 0.4490 0.5760 0.5140 0.4710 0.5280 0.4740 0.5330 0.4630 0.4280 0.4520 0.4420 0.4030 0.4450 0.4070 0.4060 0.3920 0.4290 0.4620 0.4850 TRANS VALUE 0.4540 0.3290 0.5400 0.5240 0.4950 0.4490 0.5760 0.5140 0.4710 0.5280 0.4740 0.5330 0.4630 0.4280 0.4520 0.4420 0.4030 0.4450 0.4070 0.4060 0.3920 0.4290 0.4620 0.4850 Ql P & L 41

. I-e P-

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Growth File:

111803

.PPG Transform:

NO TRANSFORMATION Summary Statistics on Data TABLE 1 of 2 I

GRP IDENTIFICATION N

MIN MAX MEAN I

1 0 LWC 4

0.3290 0.5400 0.4618 2

1.59 %

4 0.4490 0.5760 0.5085 3

3.18 %

4 0.4710 0.5330 0.5015 4

6.36 '

4 0.4280 0.4630 0.4463 5

12.72 %

4 0.4030 0.4450 0.4153 6

25.4 '

4 0.3920 0.4850 0.4420 l

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Growth File:

111803

.PPG Transform:

NO TRANSFORMATION 5

Summary Statistics on Data TABLE 2 of 2 I GRP IDENTIFICATION VARIANCE SD SEM C.V.

1 0 LWC 0.0092 0.0961 0.0480 20.8028 2

1.59 R 0.0028 0.0526 0.0263 10.3496 I

3 3.18 t 0.0011 0.0336 0.0168 6.6941 4

6.36 Il 0.0002 0.0149 0.0074 3.3357 5

12.72 %t 0.0004 0.0199 0.0100 4.7937 1

6 25.4 %

0.0016 0.0405 0.0202 9.1603

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Growth File:

111803

.PPG Transform:

NO TRANSFORMATION ANOVA Table SOURCE DF SS MS F

Between 5

0.0262 0.0052 2.0455 Within (Error) 18 0.0461 0.0026 Total 23 0.0724 (p-value = 0.1204)

Critical F

= 4.2479 (alpha = 0.01, df = 5,18)

= 2.7729 (alpha = 0.05, df = 5,18)

Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Growth File:

111803

.PPG Transform:

NO TRANSFORMATION Shapiro -

Wilk's Test for Normality D =

0.0461 W =

0.9401 Critical W

= 0.8840 (alpha = 0.01, N = 24)

W

= 0.9160 (alpha = 0.05, N = 24)

Data PASS normality test (alpha = 0.01). Continue analysis.

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Growth File:

111803

.PPG Transform:

NO TRANSFORMATION Bartlett's Test for Homogeneity of Variance Calculated B1 statistic = 11.2234 (p-value = 0.0471)

Data PASS BI homogeneity test at 0.01 level. Continue analysis.

Critical B = 15.0863 (alpha = 0.01, df =

5)

11.0705 (alpha = 0.05, df

5)

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Growth File:

111803

.PPG Transform:

NO TRANSFORMATION Dunnett's Test TABLE 1 OF 2 Ho:Control<Treatment fD TRANSFORMED MEAN CALCULATED IN SIG GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0.0' 1

0 LWC 0.4618 0.4618 2

1.59 't 0.5085 0.5085

-1.3058 3

3.18 %

0.5015 0.5015

-1.1103 4

6.36 %

0.4463 0.4463 0.4329 5

12.72 t 0.4153 0.4153 1.2988 6

25.4 %

0.4420 0.4420 0.5517 Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05, df = 5,18)

Title:

Southern Nuclear Plant Vogtle Eff. 11/18/03 FHM Growth File:

111803

.PPG Transform:

NO TRANSFORMATION Dunnett's Test TABLE 2 OF 2 Ho:Control<Treatment NUM OF MIN SIG DIFF

% OF DIFFERENCE GROUP IDENTIFICATION REPS (IN ORIG. UNITS)

CONTROL FROM CONTROL I

1 0 LWC 4

2 1.59 0i 4

0.0863 18.7

-0.0467 3

3.18 %

4 0.0863 18.7

-0.0397 4

6.36 i 4

0.0863 18.7 0.0155 5

12.72 %

4 0.0863 18.7 0.0465 6

25.4 %

4 0.0863 18.7 0.0198 Ia 1

Water Flea Effluent Test

I.

Client Southern Nucfear IDischargePlant Voatle Effluent ILocation Waynesboro, GA Cup #1 DMW Cup; MACTEC ENGINEERING AND CONSbLTING, INC.

BIOTOX LABORATORY Daily survival and reproduction data for ceriodaphnia dubia chronic test Dilution Water 1D:

C. dubia Source ID:

Template ID:

Cup #3 3.18%

DMW01111703 CERB01 110703 Cup 1#4 =

6.36%

Test Set-up Date (day 0):

fll/ 81/0?

Test Set-up Time:

Test Set-up Analyst:

gad_______

Cup#5 =

12.72%

Cup N6 =

25.44%

112=

1.59%

Day 1' Day2_________

Day 3:

I Day 4:.jj2I-,

Day 5:...4,3ail..

Day6:j4W Day 7:

rime-jjill &I Tmne:_________

. Time:__________

Tim 6

Tie-.1.

Tiwne:

.u.+/-r....

Time:_____

/C~-

t%%P W,,t~.

rid.."

IntMO.

-M

~

Infr... e lnit..

b.Pi Initq-*

a

Inft, Ur m fl n Pmhrvn A" iv Mul.

At.

r

.MA=melwnut

.fl rudduSA

= mnmll Aiduft ay~

Cup 6 Cupi Cup2S Cup 36

Cup.6
Cup 6S Day

--CuplO

.Cup 20

.Cup 30 Cup 40O Cup60 1 Cup go 0

e -__

_ 1.

I 6

4 Day I Cup 4 1Cupi 14

.Cup 24

-Cup34.:

.Cup44

-:Cup

6.

1-Day.

Cup S CupiS Cup 29 Cup 39 CupS Icup B9 4

a 3

-4 I

t/_

i It-uI

~

I I

Da Cup2.

Cup2 13 Cup 23 Cup 32 Cup 43 Cup62-.

Day Cup?:

8 Cupi

-8 Cup 27 Cup 38

-Cup 48 Cup 68

_ Ii 0.

U.

L, L

I C(- ~

Day Cup 2 Cup 12 Cup 22 Cup 32 Cup 42 Cup 62 Day CupE 7

-cupl 17

-Cup27 Cup3S Cup dG Cup 67 0

j ~....

'4 '

A.,

2 L-

~

- T

~

2 4

3.ZZ

'1'-.7 Zz 7Z i

3 i 0 L

  • 6 v

.. A-V 17.

VL.2 6 -

v-

-75

~

3 ZZ.Zj IZI Da u

u 1

Cp2 Cp3 u

1 Cp6 a

Cup J

Cup__

_:C__P2_

Cup 36 Cup 46 Cu f i

_____Temperature

(-C)

~

DyCu#1 Cull1 Cup #21

-Cup# 31 Cup 41 Cup l l up

-I u

10-.5

'0. I

.. 1..

~ %'

  • S iV(~

L Vet Location SamplelID-Sun-Mon:

0-C r00 Tue-Wed:

a_____La__

Thu-Fri:

-wi 1I

MACTEC ENGINEERING AND CONSULTING, INC.

BIOTOX LABORATORY Cerlodaphnina dubla 3-brood chronic test de-randomizatlon sheet Client-Southern Nuclear Dilution Water ID: DMW01 I 11703 Test Set-up Date (Time): 11118103 (1440)

I scharge:

Plant Vogule EMuent C. dubia Source ID: CERB01 110703 Test End Date (Time): 11124103 (1245) 1ocation:

Waynesboro, GA Template ID:

  • Test Analyst(s): ASPIMRF Cup ei DMW Cup #2 1.59%

Cup #3 =

3.18%

Cup #4 6.36%

Cup #5 12.72%

Cup #6 25.44%

MC mltn arpae dadadlt

-Nno dadnenaes V

mleadlt.E

=epBehavauliNornenaesonboto

[ItE = molten embryo A a alive adult N=no. liv neonates MF = missing adult GA = gravid adult SA = small adult C oU ncrpc edaut

-N-n.da ents Yaml dl A-ehpilautN entso ao onc:

DMW otl.

~~~Da y'Neonates, Cup#

0 1 J2

3 4

5 61 :7 I

A A

A A

3 4

1 8

12 A

A AA 4

7 12 23 53 A

A AA 3

8 13 24 24 A

AA A

2 7

1 20 45 A

A A

A 3

4 6

13 36 A

A AA 3

7 6

16 17 A

A A

A 4

9 10 23 58

'Al A

AA 6

18 39 A

A A

3 5

11 19 10 A

A A

4 r

1 M~a o of f~mw.:

1 0 U.9 nwm-gs pw f.me.

18.21-182 _

onc:

. 3.18%

Total,

~D yNeonates Cu 1.]

2 [.3 4

5 6-7 21 AAAIA4 118 23 32 A

A A IA 3

8 12 23 3

A 225 54 A

A A

A 4

9 21 15 A

A A

A 2

7 514 46 A

'3 6

11 20 7

A A

A A

6 9

621 28 A

A A

A 3

5A 8

49 A JA AA 3

812_

23 20A AA

-A 486 4

16 ng=.f no ofg~n,~..10 u.no o pn..:19.4 194 1onc:

12.72.

Total 2

~Day Neonates Cup 0

.1

.2 3-4

5.
6:.

-7 41 A

.. L.A.

A A

2 7

12 21 2

A A

A A

3 8

7 18 23

{A A

A A

2 5

13 20 34 A

A A

A 4

10 15 29 55 A

A A

A 4

5 15 24 156 j

A A

A 5

5 13 23 27 A

A A

A 3

7 9

19 L

48 j

A A

A A~~

~~

51329 9 { A A

A A

~~~4 1 11

(

27 60 IA A

4AI I

9 n1 no of ln~ols1 0 1 Li.. n.OnMo.s f..nd:

-22.9 229 Conc:

1.59%

.Total Day

-Neonates Cup#

0 1

2 33 4

5 6

7 I___

.1 A A

A A

3 9

1 1 123 42 A

A A IA 4

10 141 28 33 A

A Al A

3 8111 22

.4 A

A A

A 4

8 [I11 23

25 A

A A

A 1

8 61 15

.56 A

A A

A 3

6 71 1 6 37 A

A A

A 4

7 17 8

A A

A A

3 9

___23

.29 A A A

A 119112 23

-50 AtLA -jA IA

_ 2_

2 2ioloof.nu.

10 juen~onwasp.rf.mai.:

19.21 192 Conc:

6.36%

Total1

~~~~~~D y

Neonates 31 A

A A

A 1311 8

22

52 A

A A

A 3

11 22 13 A

A A

A 2

8 11 21 44 Al A A

A 3

5 8

16

.5S A

AlA A

4 8

5 17 26 A

A A

A 3

6 7

16 47 A

A A

A 4 10 13 27

.18 A

A A

A Sf4 6

15

.59 A

A A

A 219 14 25 40 A

A A

A 5f8 9

22 no.0 l.of o 1

0 1 U.. -nef.*~ P., f.MWO:

20.3 203

-~~~

Oa~Dy Neonates CupU 0

1 2_

3 4

5 67 51 A

A A_

A_

3 9

8 20 22 A

A A_

A 3

9 1 2 24 43 A

A A_

A 3

1 112 26 14 A

A A

A 4

8 12 24 35 A

A A

A 3

4 10 17 6

A A

A fA 3

7 12 22 57 AA AtA 2

8 7

17 19 A

AAIA 3

9 I1 1 23 30 A

A Aj_

4 1 1 W 25 Ong.

m.of o-W:

10 IU~.nm.

M.MPo tmwe:

21.41 214 Er...dby:

Q Chock.d br.!y 1 1:'

Fathead Minnow Reference Toxicant Test

M M

M M

'W.

prorWhronP RefctoxicVPMa P

M M

M M

~

~

~

ITEST NO.[.

iTSAI nDATh.. lIC2 Surv JM MEAu CUM SD UPPER CONTRO OWER CONTR C25 GrowIUM MEN+ CUM SD PPER.CONTROOWE CONTROL.

A:rS.

ic~s~:

sv:! Sr t

SD)

}

iC2

+;.. 25c G liC SD A:.2CMS 128 RFPIASP 112212002 2783 2780 2241 2240 129 RFP/ASP 2126n2002 1908 2350 620 3590 1110 2241 2240 0

2240 2240 130 RFP/ASP 312612002 3636 2780 860 4500 1060 2821 2430 330 3090 1770 131 RFP/ASP 4n2312002 2800 2780 710 4200 1360 2673 2490 300 3090 1890 132 RFP/ASP 7W/2002 4868 3200 1120 5440 960 3095 2610 370 3350 1870 133 RFP/ASP 611812002 5400 3570 1340 6250 890 2669 2620 330 3280 1960 134 RFP/ASP 7/30/2002 1900 3330 1380 6090 570 1690 2490 470 3430 1550 135 ASPIRFP 812712002 4250 3440 1320 6080 800 2003 2430 460 3350 1510 136 RPP/ASP 9/2412002 3117 3410 1240 5890 930 1950 2380 460 3300 1460 137 RFP/ASP 10122n2002 5741 3640 1380 6400 880 3937 2530 660 3850 1210 138 RFP/ASP 1112612002 4904 3760 1360 6480 1040 4396 2700 840 4380 1020 139 ASP/RFP 12111/2002 3544 3740 1300 6340 1140 2525 2690 800 4290 1090 140 RFP 112812003 3077 3690 1260 6210 1170 3167 2720 780 4280 1160 141 MRF 212512003 4144 3720 1220 6160 1280 2932 2740 750 4240 1240 142 ASPIMRF 3128103 3143 3680 1180 6040 1320 1928 2680 750 4180 1180 143 MRF/ASP 4/292003 4802 3750 1180 6110 1390 3624 2740 770 4280 1200 144 MRF/ASP sn120o1 3

2475 3680 1180 6040 1320 2882 2750 740 4230 1270 145 ASP/MRF 7/2003 3112 3640 1150 5940 1340 2084 2710 740 4190 1230 146 ASPIMRF 911612003 3143 3620 1130 5880 1360 3228 2740 730 4200 1280 147 ASP/MRF 10/21/2003 3182 3600 1100 5800 1400 2135 2710 720 4150 1270 Chronic Fathead Minnow NaCI Reference Toxicant Control Chart - Survival 8000 7D0D0......................................................................

3000................

01 2000C 1D00 A-0 Test Date Chronic Fathead Minnow NaCI Reference Toxicant Control Chart - Growth eooo 7000 gmC..................................................................................

5"..................................................................................

_4000........................

3000 2000 T000.-.........-..'............

2000 Test Dat.

I --*- tC25 Surv.

M UPPER CONTROL --a-LOWER CONTROL I I +5u IC25 Grown.

.UPPER CONTROL

-a-LOWER CONTROL

v I I.

Water Flea Reference Toxicant Test

m d

C Na-

-T-C. dubia Chronic NaCI Reference Toticant Dala Test No,

itR Date 25 S u

n Ivc Surnuitive Men cum Sp PPER CONTR OWER CONTROL 1

__j

____~C 103 104 105 106 107 108 109 110 t11 112 113 114 115 116 117 118 119 120 121 122 RFPtASP RFP/ASP RFP/ASP RFPIASP RFP/ASP RFPIASP ASP/RFP RFP/ASP RFP/ASP RFP/ASP RFP/ASP RFP RFP/ASP MRF/ASP MRF/ASP MRFIASP ASP/MRF ASP/MRF ASP/MRF ASP/MRF 2n26n2002 3126/2002 4n2312002 S/30Q2002 6/1812002 8/812002 8s291200 9130/2002 1012312002 1116w2002 1212612002 1128003 2/2112003 3/3112003 412912003 S/20/2003 613012003 7/3112003 91162003 1012112003 2750 2071 1800 1650 1650 750 1591 1859 2750 2125 2824 2333 2667 2750 3000 900 2571 2750 2461 2333 2750 2410 2210 2070 1980 1780 1750 1770 1870 1900 1980 2010 2060 2110 2170 2090 2120 2160 2170 2180 480 490 490 460 650 600 560 610 580 620 600 600 610 630 690 670 670 660 640 3370 3190 3050 2900 3080 2950 2890 3090 3060 3220 3210 3260 3330 3430 3470 3460 3500 3490 3460 1450 1230 1090 1060 480 550 650 650 740 740 810 860 890 910 710 780 820 850 900 745 1287 543 876 1020 490 1057 1043 1286 1180 1247 1327 656 1358 1133 571 1151 1045 1199 823 750 1020 860 860 890 830 860 880 930 950 980 1010 980 1010 1020 990 1000 1000 1010 10W 380 380 310 280 300 290 270 290 280 280 290 290 300 290 300 290 290 280 280 1780 1620 1480 1450 1430 1440 1420 1510 1510 1540 1590 1560 1610 1600 1590 1580 1580 1570 1560 260 100 240 330 230 280 340 350 390 420 430 400 410 440 390 420 420 450 440 Chronic C. dubia NaCI Reference Toxicant Control Chart - Survival 4000 3750 3500..............................................

3250 3000

  • t....

2500

,^2250

...... /.....

E2000..

  • ...****...****./.***....****..

1 *** ****

eit750 z1500 1.....

1250 1000

.\\..................

750 \\................

4--

500...

250..

0 T st Date 1-4-Seriesi --

See3 -*-Seies4 Chronic C. dubla NaCI Reference Toxicant Control Chart - Reproduction 13 3750......................................................................

3500 3250 3000 2750 2500.......................................................................

2250 2000 1750 1500 1250_a\\

1500.ft.

250

  • 500,,,

Test Date E-Se'1est-4 IiSefies3 SF 'i