Text

Submittal of Discharge Monitoring Report for September 2016
	ML16287A416
Person / Time
Site:	Sequoyah
Issue date:	10/10/2016
From:	Pratt P P; Tennessee Valley Authority
To:	Hall A; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Resources
References
	Download: ML16287A416 (97)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 October 10, 2016 Ms. Angela Hall Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11th Floor Nashville, Tennessee 37243

Dear Ms. Hall:

TENNESSEE VALLEY AUTHORITY (TVA) -SEQUOYAH NUCLEAR PLANT (SQN) -NPDES PERMIT NO. TN0026450

-DISCHARGE MONITORING REPORT (DMR) FOR September 2016 Enclosed is the September 2016 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period. The Toxicity Report for sampling conducted July 31 -August 5 is enclosed.

If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714.

I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.

reston P. Pratt (Acting) Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures):

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 PERMITTEE NAM E/A DDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NP DES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F -FINAL Form Approved. OMB No. 2040-0004 Add r eSL ----------

--___ _(lNTEROFFICE OPS-SN-SQM) _______ _ TN0026450 101 G _ _ _

DAISY ._I.N_l738L_

_______ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE EFFLUENT FacJilly_

..JYA *.filOUOYAH NUC LEA R PLANT_ -----L ocat i o.!!_ _!:!AMIL TOJiCOUNTY....

_________

_ ATIN: Millicent Garland PARAMETER

[:><: QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM TEMPERATURE, WATER DEG. SAMP LE ******** ******** ******** ** CENTIGRADE MEASUREMENT 00010 1 0 PERMIT ******** ******** **** ******** EFFLUENT GROSS REQUIREMENT TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** .. CENTIGRADE ME A SUREMENT 00010 z 0 PERMIT ******** ******** **1111r ******** INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** .. UPSTRM DEG.C MEASUREMENT 00016 1 s PERMIT "'*******

- - - "'*"'"' **** "'**""""""*

EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** /g 11 03 TREATMENT PLANT ME A SUREMENT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** EFFLUENT GROSS REQUIREMENT DAILY MAX FLOW, IN CONDUIT OR THRU S AMPLE 1807 ******** ******** TREATMENT PLANT ME A SUREMENT 03 50050 1 0 PERMIT Req. Mon. ******** MGD ******** EFFLUENT GROSS VALUE REQUIREMENT MO AVG CHLORINE, TOTAL RESIDUAL S AMPLE ******** ******** ******** .. MEASUREMENT 50060 1 0 P ERMIT ******** ******** **** ******** EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE

-C, RATE OF S AMPLE ******** 0.1 ******** 62 CHANGE MEASUREMENT 82234 1 0 P ERMIT ******** 2.0 DEG ******** EFFLUENT GROSS REQUIREMENT QAILYMX C/HR NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th i s document and all attachments were prepared under my d i rection or supe rvis ion in accordance w i th a system designed to assure that qua li fied Preston P. Pratt personnel properly gather and evaluate th e informat i on subm i tt ed. Based on my i nqu i ry of the ' perso n or persons who manage the system , or those persons directly respons i ble for gathering t he informa t i on , th e i nformation subm i tted i s , to th e best of my knowledge and bel i ef , true , DAY 30 *** NO DISCHARGE D ... NOTE: Read instruct i ons before completini:i th i s form. QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS ******** 37.4 0 30 / 30 RCORDR 04 ******** Req. Mon. DEG.C. CONTI CALCTD DAILY MAX NUOUS ******** 29.4 0 30 / 30 MODELO 04 ******** 30.5 DEG.C. CONTI CALCTD DAILY MX NUOUS ******** 2.1 0 30 / 30 CALCTD 04 ******** 3.0 DEG.C. CONTI CALCTD DAILYMX NUOUS ******** ******** 0 30 / 30 RCORDR .. ******** ******** ..... CONTI RCORDR NUOUS ******** ******** 0 30 / 30 CALCTD 03 ******** ******** MGD CONTI CALCTD NUOUS 0.019 0.038 19 0 22 / 30 GRAB 0.1 0.1 MG/L FIVE PER CALCTD MO AVG DAILY MAX WEEK ******** 0 30/ 30 CALCTD ** ******** ******* tut CONTI CALCTD NUOUS 1/1 Cl(¥ TELEPHONE DATE (Acting) Site Vice President 423 84 3-7 001 16 10 05 (Acting) Site President ac c urate , and complete. I am awa r e that there are s i gn i ficant pe na l t i es f or subm i tt i ng false SIGNATURE OF PRINCIPAL EXECUT I VE I i nformation , i ncluding the p o ss i b i lity of fine and impr i sonm e nt f or k n owing v i olalio n s.

AREA I NUMBER YEAR MO DAY TYPED OR PR I NTED CO D E COMMENTS AND EXPLANATION OF ANY V I OLAT I ONS (Refe rence all attachments here) No closed mode operation. The following inje c tions occurred: Flogard MS 6236 (max calc. was 0.03 mg/L , limit was 0.20 mg/L), Spect rus BD 1500 (max calc. was 0.015 mg/L , l i mit was 2.0 mg/L). EPA Form 3320-1 (REV 3/99) P revious ed it ions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different}

Name _

___ _ Add res.§_ __E.Q,_BOX 2000 ___________

_ ___ _J!NTEROFFICE OPS-5N-S(llil

_______ _ ___

-DAISY ,_I.N_273BL

_______ _ Fac.ill!Y_

JYA -_fil QUOYAH NUCLEAR PLANT _____ _ LocatiO.!L

_!:!AMIL TOJi.COUNTY_ ----------ATIN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MON I TORING REPORT (OMR} I TN0026450 I I 101 T I l.___P_E_R_M-IT_N_U_M_B_E_R __ I I DISCHARGE NUMBER I I YEAR I From. 16 MAJOR (SUBR 01) F -FINAL Form Approved. OMB No. 2040-0004 BIOMONITORING FOR OUTFA L L 101 EFFLUENT *** NO DISCHARGE D ... NOTE: Read instructions befo r e completinll this form. >< QUANTITY OR LOAD I NG QUALITY OR CONCENTRATION NO. FREQUENCY EX OF 1-------------------+--------.--------.---

MA-X-IM_U_M _ __, __ ANALYSIS PARAMETER AVERAGE MAXIMUM UNITS MINIMUM AVERAGE SAMPLE TYPE IC25 ST A TRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 EFFLUENT GROSS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT S AMPLE MEASUREMENT PERMIT REQUIREMENT

- - - - ******** ******** ******** ******** Monitoring

- - - - .. ******** Not Required ******** **** 42.8 ******** ******** MINIMUM ******** Monitoring

- - - - .. ******** Not Required ******** .... 42.8 ""*******

- - - - M I MINUM NAMEfTITLE PRINC IPAL EXECUTIVE OFFICER Preston P. Pratt I Certify under penally of law that th i s document and all allachments were prepared under my direction or supervision i n accordance with a system des i gned to assure that qualified personnel properly gather and eval uate the informalion submitled. Based on my inquiry of lh e person or persons who manage the syslem. or !hos e persons d i rectly respons i ble for gather i ng t he information. the information submilled is , to the best of my knowledge and belief. true. accurate. and complete. I am aware that th ere are s i gnif ican t penalties for subm i tting fals e (Acting) Site Vice President

Ji nformation, i nc l uding the poss i bil i ty of fine and i mpr i sonment for k now i ng v iol at io ns. TYPED OR PR I NTED OMMENTS AND EXPLAN ATION OF ANY VIOLAT I ONS (Refere nce all attachments here} oxicity was not sampled in September 2016. I IPA Form 3320-1 (REV 3/99) Previous editions may be used (Acting) S i te Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7 001 16 10 05 I AREA I NUMBER CODE YEAR MO DAY Page 1 of 1 PE RMITT E E NAM E/ADDR E SS (Include F a c il i ty N a me/L o ca t i on if D i ff erent) Name _

___ _ Addres_L _E.Q,_BOX 200 0 ___________

_ ___ .J.!.NT ER O F FIC E OPS-5 N-S<;lli l _______ _ N ATIO N AL PO LLUT ANT D I SC HA R G E E LI M I NAT I O N SYSTEM (N POE S) DISCHARGE MONITORING REPORT (DMRI TN0026450 103 G MAJOR (SUBR 0 1) F -FINAL F o rm A pproved. O MB No. 2040-0004 ___

_______ _ P ERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMEN T POND F a cJ.!i!Y_ JYA -S EQUOYAH NU CL EAR PLANT _____ _ L oca ti O.!L ....!:!AM IL TOJiCOUNTY

_ ----------

EFFLUENT ATI N: M il licent Gar l and NO DISCH A RGE D ... NOT E: Read i nst r uct i o ns b e fo re c omo l e tinQ t his f or m. PARAMETER

>< QUANTITY OR LO A DIN G QUALITY OR C ONC E N TRATI ON NO. FR EQ U E N C Y S A MPLE EX OF TYPE AV E RAG E MAXIMUM UN IT S M I NIMUM AV E RAG E MAXIMUM UN I TS ANA LYSIS PH S AMP L E ******** ******** 7.7 ******** 8.5 0 5 1 30 GRAB ME A SUR E MENT .. 12 00400 1 0 PERM I T **"'***** ******** ** 6.0 **1111ut r*** 9.0 SU ONCE/ GRAB EFFLUENT GROSS RE Q U I REMENT MINI M U M M AX I MUM WEEK SOLI DS , TOTAL SUS P EN D ED S AMP LE ******** ******** ******** 5.9 5.9 0 1 / 30 GRAB ME A S U R EME N T .. 19 00530 1 0 P ERMIT ******** ******** .. ******** 30.0 10 0.0 MG/L ONCE/ GRAB EF FLUENT GROSS RE Q U I REMENT MO A V G DAILY M X MONTH OIL A ND GREASE S AMPLE ******** ******** ******** <5.0 <5.0 0 1/30 GRAB .. 19 M EA SUR EME N T 00556 1 0 P ERMIT ******#ntr ******** .. ******** 15.0 20.0 MG/L ONCE/ GRAB EFFLUENT GROSS RE Q UIR E MENT MO AV G D AIL Y MX MONTH FLO W , IN CONDUIT OR THRU S AMP LE 1.280 1.306 ******** ******** ******** 0 5 1 30 INST AN 03 .. TREATMENT PLANT ME A SUR EME NT 50050 1 0 P ERMIT R e q. Mon. Req. M on MGD ******** ******** ******** .. ONCE/ INST AN E FFLUENT GROSS RE Q UIREMENT M O AV G D A ILY MX WEEK S AMPL E ME A SUREMENT P ERMIT RE Q UIR E MENT S AMPL E ME A SUR EM ENT P ERMIT RE Q UIR E MENT S AMP LE ME A SUR EME NT P ERMIT RE Q U I REMENT II n NAME/TITL E PR I N CI PA L E XEC UTIV E O FFI C E R I Cert i fy under pe n ally of law that this document and all attachments were prepared under my 1:1;!l: , Pra,;doot T EL EPHONE DAT E direct i on or superv i sion i n accordance w i th a system designed lo assure that qua l ified Preston P. Pratt personnel p r ope rly gath er and eval u ate th e inf o rm ation sub m i tt ed. Ba sed on my i nqui ry o f th e person or pe r so n s who manage the system , or t hose persons directly responsible for gathering 42 3 84 3-7 001 1 6 10 0 5 t he i nformat i on , the i nformat i on submitted is , to the best of my knowledge and bel i ef , true , (Ac tin g) S it e V i ce Pr esident accurate , and comple t e. I am aware that there are sign i ficant pena l ties for subm i tting false SIGNATURE OF P RINC I PAL E X E CU TI V E I i n f ormat i on , i n clu d i n g th e poss i bility o f fi n e and imprisonment fo r know i ng vio l ations. O FF ICER O R A UTHOR I Z ED AG E N T AREA I N UMBE R YEAR MO DAY TYPED OR PRI N TED CODE COMM EN TS AN D EXPLA NA T ION OF AN Y V I OLA TI ON S (R e f e r e n ce a ll attachme nt s h e r e) EPA Form 3 320-1 (REV 31 99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) _

___ _ N ATI O N AL POLLUTANT DI SC HARG E EL I MINATION SYST E M (NPDESJ DISCHARGE MON I TORING REPORT (DMR) MAJOR (SUBR 01) F -FINAL Form Approved. OMS No. 2040-0004 Add r es_L 2000 ___________

_ ___ _flNTEROFFICE OPS-5N-SQ.Nl _______ _ TN0026450

_ _ _ __§_ODDY -DA I SY ._!_N_27384_

_______ _ PERMIT NUMBER Facjfily_

JYA -_filQUOYAH NUCLEAR PLANT_ -----Locat i o.!!_ ..JjAMIL TOli_COUNTL

_________

_ ATTN: Mill i cent Garland 16 110 G DISCHARGE NUMBER QAY 30 RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I xx I *** I YEAR I NOTE: Read i nstruct i ons befo r e comp l et i nQ this form. PARAMETER

>< QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM TEM P ERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** .. CENT I GRADE MEASUREMENT 00010 1 0 PERMIT ******** **"***** .. ******** ******** REPORT EFFLUENT GROSS VALUE REQUIREMENT DAILY MX TEMPERATURE , WATER DEG. SAMPLE ******** ******** ******** ******** ** CENTIGRADE ME A SUREMENT 00010 z 0 PERMIT ******** ******** .. ******** ******llr*

30.5 INSTREAM MONITORING REQUIREMENT DAILY MX TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** .. UPSTRM DEG.C ME A SUREMENT 00016 1 0 PERMIT ******** ******** ** ******** ******** 5 EFFLUENT GROSS VALUE REQUIREMENT DAILY MX FLOW, I N CONDUIT OR THRU SAMPLE ******** ******** ******** ******** 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** EFFLUENT GROSS VALUE REQUIREMENT DAILY MX CHLORINE, TOTAL RES I DUAL S AMPLE ******** ******** ******** .. MEASUREMENT 50060 1 0 P ERMIT ******** ******** .. ******** 0.1 0.1 EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX TEMPERATURE-C, RATE OF S AMPLE ******** ******** ******** ******** 04 CHANGE MEASUREMENT 82234 1 0 PERMIT ******** 2 DEGC ******** ******** ******** EFFLUENT GROSS VALUE REQUIREMENT DAILYMX SAMPLE MEASUREMENT PERMIT REQUIREMENT ( .., " NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Cert i fy under penally of l a w that th i s document and all att a chm e nts w a r e prepared und e r my Pre*idoot di r e ct i on o r s u p erv isio n in a cco rdan ce w i th a s y s lam de s i g n ed to assu re t hat qua li fied Preston P. Pratt p ersonnel properly gather and evaluate th e information su b m i tted. Based o n m y i nqu i ry of the pe r so n or pe rs ons who ma nage the s y s t e m , or t h ose p er so n s d i r ec tly r es p on s i b le f o r gather i ng the i n f ormatio n , the i nforma ti o n subm i ll e d i s , t o l he b est of m y k n owle d ge an d bel i e f , t ru e, (Acting) S i te V i ce President a c c u ra te , and c omplet e. I am a ware that th e r e are s ig n i f ican t pen a llies f o r s ubm i lling fals e SIGNATURE OF PRINCIPAL EXECUTIVE i n f orm at ion, i n cl ud i n g th e possibili ty o f fin e an d i mp r i sonme nt f o r kno w i ng viola t io n s. TYPED O R PRINTED COMMENTS AND EXPLANATION OF ANY VIOLAT I ONS (Reference all attachments here) No D i scharge th i s Per i od EPA Form 3320-1 (REV 3/99) Pr e vio u s editio n s m ay be us e d .__ _________________ -OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous ** .. CONTIN RCORDR uous 19 MG/L Five per CALCTD Week .. ** CONTIN CALCTD uous TELEPHONE DATE 423 843-7001 16 10 05 I A REA I NUMBER YEAR MO DAY CO DE PERM I TTEE NAME/ADDRESS (Include Facility Name/Location if Different)

___ _ NA TIONA L POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDE S) DISCHARGE MONITORING REPORT (DMR J MAJOR (SUBR 01) F -FINAL Form Approved. OMB No. 2040-0004 Add r es_L _E Q._BOX 2000 ___________

_ ___ -1J.NTEROFFICE

______ _ TN0026450 110 T

_______ _ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER EFFLUENT Fac.ill!Y_

JYA -_fil:QUOYAH NUC LE AR PLANT_ -----Locatio.D_ .J::!AM I L TOJi.COUNTy_

_________

_ ATTN: M illi cent Gar l and NO DISCHARGE I xx I *** NOTE: Read instructions before c omplet inQ th i s form. PARAMETER C>< QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AV E RAGE UN ITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS MAXIMUM IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** .. 23 CERIODAPHNIA M EA SUREMENT TRP3B 1 0 0 PERMIT *****lfr** ***"**** **** 42.8 ******** ********

PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT M I NIMUM ANNUAL IC25 STATRE 7DAY CHR S AMPLE ******** ******** ******** ******** ** 23 PIMEPHALES ME A SUREMENT TRP6C 1 0 0 PERMIT ******** ******"'*

.... 42.8 ******** ******** PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT M I NIMUM ANNUAL S AMP LE ME A SUREMENT PE RMIT REQUIREMENT SA MPLE MEASUREMENT P ERMIT RE Q UIREMENT SAMPLE MEASUREMENT PE RMIT REQUIREMENT S AMPLE MEASUREMENT PE RMIT REQUIREMENT S AMPLE MEASUREMENT PE RMIT REQUIREMENT

(\. fl NAME/TITLE PRINC IP AL EXECUTIV E OFFICER I Certify under penalty o f law that th i s document and alt attachme n ts were prepared under my T ELE PHONE DAT E di rect i on or supervision i n accordance with a system des i gned to assure that qual i f i ed Preston P. Pratt person nel prope rly gather and evalua t e th e inform a t ion submitted. Ba sed on my inquiry o f th e person or persons who manage the system , or those persons d i rectly respons i ble for gather i ng S i te Vice Pres i den t 423 843-7 0 01 1 6 10 05 the information , the i nforma ti on subm i tted i s , to the best of my knowledge and bel i ef , tr ue , (Act i ng) S i te V i ce President accurate , and complete. I am aware Iha! t he r e are significant pena l t ies for submitt i ng fa lse SIGNATURE OF PRINCIPAL EXECUTIVE I i n f ormation, i nclud i ng the poss i b i lity of f i ne and imprisonmen t for know i ng v i olations. TYPED O R PRINTED COMMENTS AND EXPLA NAT I ON OF ANY VIOLAT IO NS (Refer ence all attachments here) No Discharge this P eriod EPA Form 3320-1 (REV 3/99) Previous ed i ti ons may be u sed OFFICER OR AUTHORIZED AGENT AREA I NU MBE R YEAR MO DAY CODE 0'3nO 1 r./ 1 PERM I TI EE N AME/ADDR E SS (Include Facilit y Nam e/L o ca t i on if D i ffe rent) Name _

___ _ Add r es,L

___________ _ ___ _(L N TE R O FFICE OPS-5 N-S(lli l _______ _ NATIONA L POLL U TANT D I SC HAR GE ELIM I N A TIO N SYST E M (N PDESJ DISCHARGE MONITORING REPORT (DMR J TN0026450 118 G MAJOR (SUBR 01) F -FINA L F o rm Approv e d. OMB N o. 2040-0004 _ _ _ _§ODDY -DA I SY J.N_]73 8 1_ _______ _ PERMIT NUMBER F a cJlj!y_ JYA -_fil:QUOYAH NUC LE AR PLAN T _____ _ L o ca t i O..!L _!:!A MIL TO Ji.COUNT Y._ _________ _ I Y E AR I DISCHARGE NUMBER WASTEWATER

& STORM WA T ER EFFLUENT ATTN: M i llicent Garland From. 16 PARAMETER

>< QUANTITY OR LOADING UN IT S A VE RAGE MAX IM UM MINIMUM OXYGEN, DISSOLVED (DO) SAMPL E ******** ******** MEASUR E M E NT ** 00300 1 0 PERMIT ******** ******"'* **** 2 EFFLUENT GROSS REQUIREMENT MINIMUM SOLIDS, TOTAL SUSPENDED SAM PLE ******** M EA SUR E M E N T ******** ******** ** 00530 1 0 PERMIT ******** ******** .... ******** EFFLUENT GROSS REQUIREMENT SOLIDS, SETTLEABLE SAM P L E ******** ******** ******** .. M EA SUR E M E NT 00545 1 0 PERM IT ******** ******** .... *******"' E FFLUENT GROSS REQUIREMENT FLOW , IN CONDUIT OR THRU S AMP LE ******** 03 TREATMENT PLANT M EA SUR E M E NT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** EFFLUENT GROSS RE Q UIR E MENT MO AVG DAILY MX S AMP LE ME A SUREM E NT P ERM I T REQUIREMENT S AMP LE MEASUR E M E NT P ERM IT RE Q UIREMENT S AMP LE MEA S UR E M.ENT P ERM I T RE Q UIREM E NT N AME/TI TLE PR I NC IP AL EXE C U TI V E OFFI CER I Certi f y unde r pe n ally of l a w t hal th i s do c ume n l and all auachm e nls w e re prepared u nd e r my d ire c l i on o r supervis i o n i n a c cordanc e w i th a sys l em d e s i gn e d t o as s ure th at qua li fie d Preston P. Pratt pe r s o nnel properl y gat h e r and eval u ale l h e i nf ormat i on subm i tled. B ased on my i nqu i ry of t h e pe r son or pe r sons w h o man age the sys t e m , o r t h ose p e rsons d i r e ctly r esp o ns i b l e for ga lh e r i ng l h e i nfo r mal i on , t he i nforma l i on su bm i lled i s , lo l he besl of my k n o w l edge a nd b e lief , l ru e , I Q AY 30 *** NO DISCHARGE I xx I *** NOTE: Read instr u ct i ons befo r e complet i n Q t hi s fo r m. QUALITY OR CONCENTRATION NO. F R EQUE N C Y SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS ******** ******** 19 ******** ********

MG/L TWICE/ GRAB WEEK ******** 19 ******** 100 MG/L TWICE/ GRAB DAILY MX WEEK ******** 25 ******** 1 ML/L ONCE/ GRAB DAILY MX MONTH ******** ******** .. ******** ********

ONCE/ ESTIMA BATCH '\r ,... I ' .

T E LE PH O N E DA T E Act i ng) S i te Vice Pres i de nt 423 843-7001 1 6 10 05 (Act i ng) S i t e Vice Pres i de nt a ccural e , an d c omp l e t e. I am aw are lh a l l h e re are s i gn i f ican t p e na l l ie s f or s u b m i ll i ng fa lse S I GNATUR E OF P R I NCIPA L E X ECUTI V E I in form at i on , i nc l u d i ng th e pos s i b i lil y o f fi ne and i m pr i s on m e nl f o r k n ow i ng v i o l at io ns. O FFIC ER OR A U THO RI ZED A GENT AREA I N UMB ER YEA R M O DA Y TY P ED O R PR I NT ED CODE COM M E N T S AN D EXPLA NAT I ON OF ANY VI OLATI O NS (Refere n ce a ll atta c hments here) Du ri ng this report i n g period , ther e has b een no flow f r om the Dred ge Po nd o ther tha n t h at r esult i ng from r ai nfa l l. N o D is charge this P er i o d E PA Form 3320-1 (REV 3/99) Previ ous edi t i ons may b e used P::.n<> 1 r.f 1 TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: August 26, 2016 I. Facility I Discharger:

Sequoyah Nuclear Plant I TV A 2. County I State: Hamilton I Tennessee

3. NPDES Permit#: 1N0026450

4. Type of Facility:

Nuclear-Fueled Electric Generating Plant 5. Design Flow (MGD): 1.579 6. Receiving Stream: Tennessee River (TRM 483.6) 7. lQIO: 3.491 8. Outfall Tested: 101 9. Dates Sampled: July 31-August 05, 2016 10. Average Flow on Days Sampled (MGD): 1791, 1797, 1793 11. Pertinent Site Conditions:

Production I operation data will be provided upon request. 12. Test Dates: August 02-09, 2016 13. Test Type: Sh011-tenn Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids ( Ceriodaphnia dubia) 15. Concentrations Tested(%):

Pimephales promelas:

UV treated Outfall IOI: 10.7, 21.4, 42.8, 85.6, 100

UV treated Intake: I 00 Ceriodaphnia dubia: Non-treated Outfall IOI: 10.7, 21.4, 42.8, 85.6, 100 Non-treated Intake: I 00 16.PennitLimitEndpoint(%):

Outfall IOI: IC 22=42.8% 17. Test Results: Outfall 101: Pimephales promelas:

IC 22 > 100% Ceriodaphnia dubia: IC2s > 100% Page 1 of90

18. Facility Contact: Millicent Garland Phone#: (423) 843-6714 19. Consulting I Testing Lab: Environmental Testing Solutions.

Inc. 20. Lab Contact: Jim Sumner Phone#: (828) 350-9364 21. TVA Contact: Donald W. Snodgrass Phone#: (256) 386-2787 22. Notes: Exposures to samples collected July 31-August 05, 2016 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids.

The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to -intake samples resulted in no significant difference from the controls during this study period. Page2of90 METHODS

SUMMARY

Samples: 1. Sampling Point: Outfall 1O1. Intake 2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY)

Arrival Initial (MM-DD-YY)

Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (oC) (mg/L) LastUsedBv 07-31-16 0705 to 08-01-16 1307 t <0.10 08-02-16 0927 101 08-01-16 0605 1.2, 0.8' 08-03-16 0857 07-31-16 0720 to 08-01-16 1307 0.2 <0.10 08-02-16 0927 Intake 08-0l-i6 0620 08-03-16 0857 08-02-16 0705 to 08-03-16 1220 1 4t <0.10 08-04-16 0906 101 08-03-16 0605 1.2, .. 08-05-16 0907 ' 08-02-16 0715 to 08-03-16 1220 1.4 <0.10 08-04-16 0906 Intake 08-03-16 0615 08-05-16 0907 \ \ 08-06-16 0939 101 08-04-16 0710 to 08-05-16 1235 0.5, 0.7t <0.10 08-07-16 0935 08-05-16 0610 08-08-16 0853 08-04-16 0725 to 08-06-16 0939 Intake 1235 0.6 <0.10 08-07-16 0935 08-05-16 0625 08-08-16 0853 *TRC = Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers.

Temperature was measured in each cubitainer upon arrival. 4. Sample Manipulation:

Samples from Outfall 101 and intake were wanned to test temperature (25.0 +/- l.0°C) in a warm water bath. Page3 ot90 Aliquots of Outfall 101 and Intake samples were UV -treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Pimephal'es promelas Test Organisms:

1. Source: In-house Cultures 2. Age: < 24-hours old Test Method Summary: 1. Test Conditions:

Static, Renewal 2. Test Duration:

3. Control I Dilution Water: Moderately Hard Svnthetic

4. Number of Replicates:

.1 5. Organisms per Replicate:

1 O 6. *Test Initiation: (Date/Time):

08-02-16 0855 ET 7. Test Termination: (Date/Time):

08-09-16 0848 ET 8. Test Temperature:

Outfall 101: Mean= 24.7°C (24.2 -25.1°C) 9. Physical I Chemical Ceriodaphnia ciubia In-house Cultures < 24-hours old . Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Svnthetic l 08-02-16 0927 ET 08-09-16 083 l ET Mean= 24.8°C (24.6 -25.2°C) Measurements:

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration.

Pre-and exposure test solutions were analyzed daily for pH and dissolved oxygen. 10. Statistics:

Page4 of90 Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software.

McKinnevville, CA).

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration)

Conducted August 02 -09. 2016 using effluent from Outfall IO 1. Test Percent Surviving Solutions (time interval used -days)* (%Effluent) 1 2 3 4 5 *6 7 Control, 100 100 100 100 100 100 100 UV-treated 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 98 98 98 98 100.0% 100 100 100 98 98 98 98 Intake 100 100 100 100 100 100 98 Control, 100* 100 100 100 100 100 100 Non-treated Test Solutions Mean Dry Weight (mg) (replicate number) (%-Effluent) 1 2 3 4 Mean Control, 0.565 0.482 0.548 0:478 0.518 UV-treated 10.7% 0.488 0.461 0.516 0.504 21.4% 0.527 0:465 0.430 0:449' 0.468 42.8% 0.481 0.509 0.537 0.528 0.514 85.6% 0.470 0.499 0.431 0.449 0.462 100.0% 0.466 0.502 0.534 0.501 0.501 Intake 0.479 0.511 0.495 0.460 0.486 Control, 0.587 0.555 0.525 0.423 0.523 Non-treated IC2s Value: > 100% Calculated TU Estimates:

< 1.0 TUc* Permit Limit: 42.8% Permit Limit 23 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso:

TUc = 100/ IC2s Page5of90 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration)

Conducted August 02 -09, 2016 using effluent from Outfall 101. Percent Surviving Test (time interval used -days) Solutions 1 2 3 4 5 6 7 (% Effluent)

Control 100 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction

(#young/female/7 days) (% Effluent)

Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 31 31 31 34 32 30 31 28 29 29 30.6 10.7% 31 32 32 35 30 33 35 29 33 32 32.2 21.4% 31 32 32 34 32 36 30 31 34 31 32.3 42.8% 34 35 35 31 33 34 32 32 37 30 33.3 85.6% 32 32 34 36 34 36 30 34 30 33 33.1 ' 100.0% 37 35 36 31 36 35 38 37 33 36 35.4 IC2s Value: > 100% Calculated TU Estimates:

< LO TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA , Lower Limit: NA *TUa = 100/LC 5 o: TUc = IOO/IC2s / Page 6 of90 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration)

Conducted August 02-09, 2016 using water from Intake Percent Surviving Test (time interval used -days) . Solutions 1 2 3 4 5 6 7

(% Effluent)

Control 100 100 100 100 100 . 100 100 *:. Intake 100 100 100 100 100 100 100 Test Solutions Reproduction ( #young/female/7, days) Data (replicate number) ; (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 33 31 29 31 28 32 31 31 30 30.5 Intake 35 33 35 32 37 38 32 36 33 34 34.5 IC2s Value: > 100% Calculated TU Estimates:

< 1.0 TUc* Permit Limit: N/ A Permit Limit: N/ A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso:

TUc = lOO/IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Species Date Time Duration To xi cant Results OC2s) Pimephales promelas August 02-09, 2016 0830 KCl 0.79 g/L Ceriodaphnia dubia August 02-09, 2016 0915 7-days NaCl 1.07 g/L Page 7 of90 PHVSICAUCHEMICAL SUM M.ARY Water Chemistry Mean Values and Ranges for UV-treated Pimepha/espromelas and Non-treated Ceriodaphnia dubia, Sequoyah Nuclear Phmt (SQN), Efiluent Outfall I OJ and Jntake perform::d Augtmt 02-09, 2016. Test Sample ID Tempemture (0 C) Dissolved Oxv11:en (m!!:IL) Initial Final Initial Final Control, 24.7 24.5 7.8 7.6 NurHrl?'utcd 24.6 -24.8 24.3 -25.0 7.7 -7.8 6.4 -7.9 Control. 24.7 24.5 7.8 1.4 UV-treated 24.6 -24.8 24.4 -24.7 7.8 -8.0 5.5 -7.9 i I0.7% 24.8 24.5 7.8 7.4 24.7 -24.9 24.2 -24.7 7.8 -7.9 _5.5 -7.9 24.8 24.5 7.8 1.4 co 21.4% 24.7 -25.0 24.2 -24.7 7.8 -8.0 5.4 -7.9 42.8% 24.8 24.5. 8.0 7.4 .i: 24.7 -25.0 24.3 -24.7 7.9 -8.2 5.5 -7.9 85.6% 24.8 24.5 8.0 7.5 24.7 -25.0 24.4 -. 24.7 7.9 -8.2 5.5 -8.0 100% 24.9 24.4 8.0 7.5 24.8 -25.l 24.2 -24.7 7.9 -8.2 5.5 -8.0 Intake 24.8 24.5 8.0 7.6 24.7 -25.0 24.4 -24.6 8.0 -8.2 6.3 -8.0 Control, 24.7 24.9 7.8 7.9 Non-treated 24.7 -24.8 24.6 -25.0 7.7 -7.8 7.8 -8.0

10.7%. 24.8 24.8 7.9 7.9 24.7 -24.9 24.6 -25.0 7.8 -7.9 7.8 -8.0 .!;! 24.8 24.8 7.9 7.9 -<:> 21.4% 24.7 -24.9 24.6 -24.9 7.8 -8.0 7.8 -8.0 .s 24.8 24.8 7.9 7.9 :: 42.8% ..:: 24.7 -24.9 24.6 -25.1 7.8 -8.0 7.8 -8.0 85.6% 24.8 24.8 7.9 7.9 24.7 -24.9 24.7 -24.9 7.8 -8.0 7.8 -8.0 100% 24.9 24.9 8.0 8.0 24.8 -25.0 24.7 -25.2 7.8 -8.1 7.9 -8.J Intake 24.9 24.9 8.1 8.0 24.8 -25.0 24.6 -25.2 8.0 -8.2 7.9 -8.2 *Note: Total residual chlorine was performed on non-treated Outfall IO I and Intake samples. Overall temperature (0 C) Pimephales prome/as Ceriodaphnia dubia Average 24.7 24.8 Minimum 24.2 24.6 Maximum 25.1 25.2 pH (S.U.) Conductance Alkalinity Hardness *Total Residual Initial Final (µmhos/cm) (mg/L CaC03) (mg/L CaC0 3) Chlorine (mg/L) 7.89 7.81 319 60 91 -7.73 -8.09 7.51 -8.06 308 -330 59 -62 88 -7.93 7.78 312 59 89 -7.82 -8.02 7.48 -7.89 301 -322 58 -59 88 -7.94 7.75 304 ---7.83 -8.04 7.49 -7.86 294 -311 ------7.95 7.76 .. 293 .... -**----7.84 -8.04 7.50 -7.85 285 -300 ------1.95 7.76 271 ---7.85 -8.03 7.51 -7.86 265 -277 ------7.97 7.77 226 ---7.86 -8.04 7.52 -7.89 223 -233 ------7.99 7.77 209 76 83 <0.10 7.88 -8.08 7.54 -7.89 205 -215 74 -78 82 -86 <O.IO -<0,10 7.99 7.81 205 73 78 <0.10 7.89 -8.07 7.65 -7.91 199 -209 72 -74 73 -82 <0.10 -<0.10 7.89 7.94 319 60 91 -7.73 -8.09 7.86 -8.03 308 -330 59 -62 88 -8.01 7.92 305 ---7.91 -8.12 7.85 -8.02 295 -313 ------7.99 7.92 294 ---7.90 -8.10 7.85 -8.01 283 -304 ------7.99 7.93 270 ---7.90 -8.09 7.86 -8.03 257 -277 ------7.99 7.95 225 ---7.91 -8.09 7.89 -8.04 216 -235 ------8.01 7.96 205 78 83 <O.lO 7.92 -8.11 7.90 -8.05 197 -215 76 -80 82 -86 <().10 -<0.10 8.00 7.98 205 73 78 <0.10 7.93 -8.10 7.92 -8.08 197 -211 72 -74 73 -82 <0.10 -<0.10 ,._.

SUMMARY

I CONCLUSIONS Exposures to samples collected July 31 -August 05, 2016 from Outfall 10 I resulted . ' in no toxic effects to fathead minnows or daphnids.

The resulting IC25 values, for both species, were > I 00 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Page9of90 Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment.

Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EP A-82l-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment.

Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL 1. Pimephales promelas Samples used in the fatl}ead minnow test were exposed to UV light for two minutes prior to introduction of test organisms.

UV treatment is used to control interference of fish pathogens.

This method was approved on November 23, 2015 by the State of Tennessee in a letter frdm Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring.

2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None *{ 2. Ceriodaphnia dubia None Page 10 of90

' PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments:

All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance.lOgbooks.

3. Temperature was measured by SM 2550 B-2000. 4. Dissolved oxygen was measured by SM 4500-0 G-2001. 5. The pH was measured by SM 4500-H+ B-2000. 6. Conductance was measured by SM 2510 B-1997. 7. Alkalinity was measured by SM 2320 B-1997. 8. Total hardness was measured by SM 2340 C-1997. 9. Total residual chlorine was measured by ORION 97-70-1977. ) QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013

.. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC 25 values in g/L KCl or NaCl. 2. Standard Toxicant:

Potassium Chloride (KCI crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia. 3. Dilution Water Used: Moderately hard synthetic water. 4. Statistics:

ToxCalc software Version 5.0 was used for statistical analyses.

Page 11 of90 REFERENCES

1. NPDES Permit No. TN0026450.

2. USEP A. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002). 3. Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012. 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 12of90 Page 13 of90 Sequoyah Nuclear Plant Biomonitoring August 02 -09 , 2016 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser D ischarge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks , During Toxicity Test Sampling, February 6 , 2005 -Augu st 5 , 2016 Date Sodilim Towerbrom PCL-222 PCL-401 Cuprostat-PF H-130M Nalco Hypocblorite mg/L mg/L mg/L mg/L m,g!L , 73551 mg!L TRC Phosphate Copolymer Azole .Quat mg/L TRC EOIPO 02/06/2005 <0.0042' 0.028 0.010 02/07/2005 <0.0116 0.028 0.0 10 02/08/2005 <0.0080 0.028 0.010 02/09/2005 O.Q199 ().028 0.010 02/10/2005 <0.0042 0.028 0.010 02 11112 005 0.0155 0.028 0.010 06 1 05 1 2005 0.0063 06/06/2005 0.0043 06/07/2005 0.01 03 06/08/2005 0.0295 06 1 09 1 2005 0.0129 06/l 0/2005 . 0.0184 07/17/2005 0.0109 0.0 26 0.009 07/18/2005 0.0150 0.026 0.009 07/19/2005 0.0163 0.026 0.009 07/20/2005 0.0209 0.026 0.009 07/2112005 0.0242 0Jl26 0.009 07/22/2005 0.0238 . , Q.054* O.Q18 10/30/2005 0.0 068 10/3 1/2005 0.0112 1110 1/2005 0.0104 11/02/2005 0.0104 l l/03/2005 0.0117 11/04/2005 0.0165 l l/14/2005 0.0274 11/15/2005 0.0256 l 11 16/200 5 0.0234 11/17/2005 0.0231 11118/2005 0.0200 l lil 9/2005 0.0116 Page 14 of90 lH50M'"

Date 11112/2006 11/13/2006 11114/2006 11/15/2006 11116/2006 11/17/2006 11126/2006 11/27/2006 11/28/2006 11129/2006 11/30/2006 12/01/2006 05/28/07 05/29/07 05/30/07 05/31/07 06/01107 06/02/07 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04115/08 04/16/08 04/17/08 04/18/08 10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31/08 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced C o rrosion and Mollusks , During Toxicity Test Sampling , February 6 , 2005 -August 5, 2016 Page 1 5 of90 PCL-401 mg/L Copolymer Cuprostat-PF mg/L Azole Nalco 73551 mg/L EOIPO 0.017 0.017 0.017 O.Dl8 H-150M quat *0.037 0.037 0.037 0.037 0.036 0.036 0.036 0.036 0.041. 0.041 *0,041 0.041 MSW 101 m gJL Phos hat e 0.015 0.015 0.015 0.015 0.015 0.015 0.030 0.030 0.030 D a te 02/08/09 01 1 0 9 1 0 9 0 2 11 0 1 09 02 1111 09 0 2/12/0 9 02/1 3/09 05/10/09 05 11 1/09 0 5/12/0 9 05 11 3 1 09 05/14/09 0 5 11 5 1 0 9 11/15/09 11 /1 6/09 11 117/09 11/1 8/09 11 119/09 11/2 0/09 05/09/1 0 0 5/1 0/1 0 05/1 1110 0 5 11 2 11 0 0 5 11 3/1 0 0 5 il 4/IO Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks , During Toxicity Test Sampling , February 6 , 2005 -August 5 , 2016 'Sodium Towerbron Hypochlorite mg/L mg/L TRC 'TRC 0.0197 0.023 7 0.0104 0.0155 0.0106 0.0129 0.0415 0.0053 0.0049 <0.0141 <0.0160 0.025 0.0152 0.0255 0.0306 0.0204 0.0093 0.0192 0.0055 0.0100 0.01 7 1 0.0041 0.0099 Page 16of90 Cuprostat -PFmg/L mWt Azole QUat H-150M mg/L Quat 0.0446 0.0396 0.0396 0.0397 I I Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling , February 6 , 2005 -August 5 , 2016 Date 10/31/10 11101/10 11/02/10 11103/10 11/04110 11105110 05/0l/20ll 05/02/2011 05/03/2011 05/04/2011 05/05/2011 r 05 1 06 1 2011 *. '. -1 11 06/2011 ll/07/20ll 11/08/2011 11/09/20 LL L L/10/2011

"'* 11/1 1/201 L 05/06/2012 05/07/2012 05/08/2012 05/09/2012 05/10/2012 05/11/2012 08/12/2012 . 08/13/2012 *, 08/14/2012 08/15/2012 08 116/2012 08/17/2012 05/12/2013 05/13/2013 05/14/2013 05/15/2013 0 5/16/2013 05/17/2013 09/15/2013 '*"' **. 09116/2013 *. 09/17/2013 09/18/2013 09/19/2013 . 09/20/2013 * ,; -Page 17 of90 PC L-401 CL-363 mg/L mg/L Copoly-DMAD. mer Nalco 73551 mg/L EOIPO 0.036 0.036 SpecttuS. H-150M. MSW CT1300 mg/L d 01 mgJf ' Quat mg/L Quat . Phos-0.04 0.04 . 0.04 0.04 hate .-', Flo guard MS623 6 mg/1. Phosphat e 0.029 0.029 0.02 9 0.029 0.029 0.032 0.064 0.064 0.064 0.032 o.o:u 0.03 0.03 0.03 0.03 O.Q3 0.03 Date 05/04/2014 05/05/2014 ,: 05/06/2014 05/07/2014 05/08/2014 . 05/09/2014 09/07/2014. 09/08/2014 09/09/2014 09/10/2014 09111/2014 09/12/2014 08/09/2015 08/10/2015 08/11/2015 08/12/2015 08/J 3/2015 08/14/2015 10/18/2015 10/19/2015 10/20/2015 l 0/21/2015 10/22/2015 l 0/23/2015 . 05/15/20 16 05/16/2016' 05/17/2016 05/18/2016. 05119/2016 05/20/2016 08/01/2016 08/02/2016 08/03/2016 08/04/2016 08/05/2016 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5 , 2016 0.0118 0.0112 0.0096 0.0164 0.0235 0.0110 Page 18 of90 Flo guard MS6236 mg/L 'ii* Phosphate 0.03 0.03 0.03 Page 19 of90 Sequoyah Nuclear Plant Biomonitoring August 02 -09 , 2016 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Pect Name: Sequoyah NP Toxicity Number: N/A Sampled: Sequoyah NP NPDES Number: TN-0026450 SQN-101-TOX Comp Comp BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of _I_ Environmental Testing Solution, Inc. 3 51 Depot Street. Delivered By (Circle One): FedEx UPS Bus Client Asheville, NC 28801 Other (specify):-::OY\iC..

\*el Phone: Fax: Collection Date/Time SllUt 11*1 ,, /{; o'loS:..

'? .J/-/{., 01to<U' /JfP. 20e 828-350-9364 828-350-9368 2(2.Sgal) 1(2.5 gal) Flow (MOD) l'1<ff>, gJG, Yes x Rain Event? (Mark as Appropriate) tfYcs, No Inch ca \ .\3 Trace Sample Custody-Fm in From Top Down Date/Time Reee.ived By (Signature)

ETSLog Number Laboratory Use Arrival Temp. By Time

Appear* "C &!ICC Tn$tru(:tion$:

Clients Should tlll in all areas except those In the " Labora10ry Use" block. Bi<lt11oilitoring samples arc preserved by storing them between 0°C and 6°C and shipping them in ice (samples should never be fr pzcn), ihehold time for each $J1llplc is 36 from the time of eollection.

Therefore; please collect and sbip in such a that the laborlitory will receive. the samples with amjjle time to inltiat'e testi n g With i n that time frame. Samples shipped overnight on Friday via FedEx or UPs must be marked for Saturday delivery or they will not mive until the followirJg Monday.

r Whole Effluent Sample Receipt Log *sample temperetllre performed 111l11C S.mplt Rece. vln1Tn11mometer.

$N Date Time Received Received *sample* Project Sample s.ample name and description State C:ommenu Received by front temp. 1°c) hUli'll;ler numbet. -011;01-lfi 1013 K. Keenan J. Kennedy '4.0 l6Q801.0I Tu , cl<<tsel1ee WWTP NC *-08*0H6. 1307 J. Sumner TVA Courier 1.2/0.8. 1163'7 1:60801 .02 TVA-Sequoyah NP-101 ... TN 1307 J. Sumner TVA Courier 0.2

.03. 'TVA.* $eauovah NP -Intake -TN SOP G4 -Exhibit G4.2, revision 01-03-12 -------------------

aent: TVA !i>ject Name: Sequoyah NP Toxicity N/A Sampled: Sequoyah NP NPDES Number: TN0026450 C II dB*. Ma.rcc,;.5 O-.>*?ccrc..

r o ecte y: SQN-101-TOX Comp Comp Relinquished By (Signature}:

BIOMONITORJNG CHAIN OF CUSTODY RECORD Page 1 of 1 ----Environmental Testing Solution, Inc. 351 Depot Street. Phone: Fax: Asheville, NC 28801 828-350-9364 828-350-9368 Delivered By (Circle One): FedEx UPS Bus Client Other (specify):

Son \<!. JJe \ i t Container Number & Flow Volume (MGD) Collection

Date/Time Rain Event? (Mark as Appropriate) I \.\o!l-Laboratory Use Collected

.....

S1a:11 End Yes No Trace D&D5r.; x* o.o3 /& , 3*16 0? J $" "£1: 0 Io e.T. x 1(2.S gal) Sample Custody"-Fill ln From Top Down Date/Time Received By (Signature):

So.""\C..

l:.O.\.<<.tj ETS g ArrivalTellljl

. By Number *c l 2:3 \.'2. \A *c... \. '-l 'L Tlm*e h.1..() \"1.. '1. o er Appear* BQCC i Instructions

Clients should fill fn all areau.xcept those in the **Laboratory Use" block. Biomonitoring samples 11rc preserved by sto-ring*thern at 6°C and shipping them in ice. Thehold time fQr each sample

is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefote.

please collect and ship in such a way that the labpratory will receive the samples with ample time to Initiate testing within time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked fot Saturday delivery or ihey will not arrive until the following Monday.

Whole. Effluent sample. Receipt Log Date Time'

*$llmpia 'Project ..

Sample name.a11d descriptfi)it

' *Commehts Received .. .

by .ftdrn: .. .

i:iumb!ae'

'0940, K>Keenan.

ues: ...

(" 11650 . aooaps,,01

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0940 . K Keenan. ups; 1.l1; 11651 160803' .02 . SoLithiCarv WWTP NC 08*03"3;6: . . 05140: l<iKe1man .UPS. " ** 1;3' 11652* . 160803 .* 03 .. MoceheadCltv WWTP** <NC ...

'1021' Fed-EK .. . , 2.2 .. 11653 . 160803: ,04 BeleWs.CrilekSS, " .,,., : NC * .. .

1021 . K. Keenan . ,Fed.-Eic 1;5 11654 .. 160803.0f BrunswlckCountvWTP

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K.Keenan Fed*.El<..

1.0.: 1* 11655' 1608l>3 *06 Oi:>bsonWTP NC.

1021 {(;Keenan.

Fed.-.Ei<

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... NC. 0S;.03,1s 1021:. .* K. Keenan** Fed* Ell 2;1 UGS.7 . 160801r.08<

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NC' 08'03-16'.

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!;:J! 116Str . 160800 .* 09* Jameslougtilrit WWTP NC OS*OlHG 1021; .K.,Keenan Fed,-£x, l.O 11659 ... 16080L l 0> Performance fibers NC:. . 0&-03*1.6 . .

1021 .K. Kee(ian* .. 'Fed*E'f<

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Releillh*CC.

NC: .oa-oa;.16, 1021 K;Keenan.

Eed*Ex* to .'11651, .. 1S0803 ,ti soarks RoadWTP ** N.C 1021 !(;:Keenan, ;fed,.,£x; 1:() 11662 .. 160803 ;J3: .; lone CreekWWTP NC '

1021.* K.ilteenan*

Fed'-Eic l;S 11663' 160803 .14* McGUlre NS .iQOf' NC .08*03-16 1021 K.Keenan 'Fed*EX 1.0. 11664 . . 160803; JS :*. McGuire.NS;.

002 NC os*'O.!H&

.1021 K.'Keenan*

Flid*E1c i.o 160803 .16 ManteoWWTP NC 08'*03"16 1021-. It Keenan Fed-Ex** ***1A 11666 .. ' 160803".1'7 Mavo steam Electric Plant NC

... 1021 !(;Keenan Fed-_Ex* 2.6: 11667 160803 .* 18 Wai!'hln:liton.WwrP*

NC .,._

1021

.. Fl!d.::;*E)<* . .13 11668 . '160803 .19 Woodliik'e.

Yacht Club* *.:NC.

.1021 l(,l(eenan

2.1 '11669

1&osoa* .ao PCS*Phosphate; Inc. NC*

.1021 K. Keenim flt. ,n 11670 '.1'60803'

,21 Carolina Beach WWTP NC QS-03*16 *1oss K.Keenan*

J.Kennedv 4.0; 11636 l608Q3 Tuckaselgee WWTP NC 08*03-16 .1220 J;sumner .TVA*Courler "1:2/1.4 11631 160803* ;23 TVA NP

101 TN 08*03;16 1220 J *. sumner TVA courler

1.4 .11'637' .160803* ,24 JVA;-Sequovah.NP'

TN

- 1zss . K. Ke-enan' .. Dash Courier 0'.3 . 11671 160803 .25: OWASA NC *O&!l3-16 1500' J:Sumner TVA Courter .O.S': .. 11672: 160803 ;26 TVA* Shawnee fp.,. OOlAM KV-08*03-16*

1500 J.Sumner TVA Courter o;9' ... 11672 1'60803 ,27**

Shawnee FP'* f ntake AM KY* I 08*03*16 1500 .J,Sumner TVA Courie( ;*o.a: . 11672: 160803 ;28 TVA' Sha\Nnee FP'. 001 PM KV* .. 08-03-16 . ... 1500.* J. Sumner * .... ,,. TV.A Cci.urler

1.3 11672 160803' .29 .. TVA .. Shawnee rP .,,, KY SOP G4 *Exhibit G4.2, revlsion 01-03-12

., .. --BIOMONITORING CHAIN OF CUSTODV llECORD Page_l_ of _1_ .. 1 Client: TV A " EnvironmentaJ Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity Asheville FedEx Location.

FedEx UPS Hus Client . -P.O. Number: NIA 628 Patton Ave. Other (specify):

c AsheviUe, NC 28806 General Comments:

{fj @ z .... r VI Facility Sampled: Sequoyah NP 071 c i* NPDES Number: TN0026450 Phone: 828-350-9364 ht;i:.5(A?1/t'.$

()itk lei t

v Fax: 828-350-9368

'./--* **: _ _,._ ,,. \ £' "* .,,.. A 0

Mid t..Jet'(

J:eJ)I p>-r_J,bt:_

v v ./ '*

I F I Conu1incr Field ldcnlilJcation I GrabiCornp.

Colieciion Dateffimc

'Number& Flow Rain fiv.cnt? Sompic Description Volume (MQD) l.aboratary Use Cull1:c11:d (Mork as Apprnprfaic)

-Stnn "F.m.1 Yes rrv,s, No Trace F.TS log Aftivol Temp. 'Ry Time-Appear* -** --Inches Nutnbcr ("Cl . a-once

$-5', I<., x lbitJoSIS o.s o:'\'C. a' SQN*IOl*'rOX Comp 2(2.5gal) o.L\'t . -;.

I SQN*llff *TOX It.:. -x *o.t.ft

t. \1.)S .,_ co,np 5 I 1(2,5 gaJ) ff}J!I D1Z.5e . DIPZ..

Sample Custody-Fill In ftom Top Down UJs"U>>l>"'I StA\S llilT'f!(.M.

SN-\J\e$

Sy (Signature):

Da(e/Timc Received by (Signature):

U,U\ )6) &Cltlb _

Oate/Ttme 1to) 1\\,.1 ' ,_ '.wa: _-. *-" / . Y*Gik/t>3 C.OE ((-,, ...

U'

() ./ . . f:," r t 9--1 ii..

CJ y-..

...

tr r '-J .. lnstructie>ns:

Clicms should 1111 in illl arcas except those in the "l.obnratoey Use" bfilck. Binnionitnring sumplcs ore preserved by storing them between 0°C and 6°C and shipping lb.em in ire (snmplcs sJ1ould nllver be fro7.eo).

The iiold time ror cuch sample Is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from theJhne of collection.

Thercforii, please collect nod ship in such IJ WU)* that the faboratory will receive lhe s:implos with umple lime lo initiutc !csllng within thnt tirnc frame. Sample:t shi11i1cd 1wcmight on Friday via FedR" or UPS must Ile marked for Sowrday delivery or Ibey will not. orrl\'e until the lbllowlng Monday.

('-. Whore Effluent Sarnple Receir;t Log Date Time Received CQ C'D !Jecelved.

Received bV. Sarnple name and description

PfoJect ftom . . *numlli!t*

nlimbet Sample l'I.,)

:0946 Kd<'eeoan 01 0946 0

..... ::.1011. .. , -*-K.: Keeiien* *. ..... \JPS.. 0.6 . ..11650 .... : :.160805 ;OJ: Ror::khighamWWJP:

NC-ex.

J:a 11653 . <160B'OS ,.;03 . Belews Creek ss

Ne (0 l011 .. K.Keenrin**

Q .. OS*O!i*l6 1011 K.Ktlenari

- Fed Ex 1.1 11655 160805:.05 D6bsonWTP NC 08-.05-16.

1011 .K;Keenail Fed* !iii *** a.s 11656 *1aosos Marshiil!Ss

Ne. 08-05*16 101r* : t<l Keenan.

Fed ,,ti(. .o.s 11657_ *. *160805 ,07 North Cary WWTP NC 1011 . K* Keenini . F.ed. E'X o.a 11658 . -J60BO!F.()&" JarnesfoughllnWWTP

.. NC 1011 K.Keel'ian 1011 1011 Fed -t:lf* 1.1 11659 1608US' .09 Performance Fibers *

NC *os,os-1.s._

1013,:. K;Keellan 08*05*16:

1011 It Keenan 1203 H* Bingham 1.3.' 11636;

160805 J4

Tuckaseli;iee WWTP' NC 1235 * .... J.,Sumner:

TVA Coufler O,S/0.7 .. 11637 160805'. .15: TVA -Seauo\.iah NP . ' TN '08-05-16 1235 J. Sumner* TVA Coorier D.6 11631 160805 , 16 1VA NP* Intake TN 08-05-16*

1301

J. Sumlier Dash toifrler , 130M ** i: 11s1:r 160805 .1 T *. OWPSA Mason i:am1s . NC SOP G4 -Exhibit G4.2, revision 01-03-12

q *.ik.:.-,

....

l Qf'l I. ;' Chronic Whole Effluent'Tr,xiciq

..

to,(l.U}

Species:.

PiHtep[zales

Client: Tennessee.

Vallev Auth&r.ity Facility; Sequoyah Nuclear Plant* NPDES #: TN002645(l . . . .

Dilution orevarn.tion i1tfQr11.iatf(Jn:

Dilution prep (%,) 10:1 .. 21.4 Effluent volume (ml.}.

535 Diluen1.voh,m1e.(1nL) 42:$ 1()70 1430. Total *volume {ml:.) 2SOO :uoo** is(}()*' .. Test Organism source:

In-hctuse culture Age: < 24-bours old Snawndate:

.*

t.2'-\\:, . 8$:6, '2140 360. 2500 Hatch dates and l<Stit>--re .roo 2$00 0, 2500 o Transfer vessei infonnation;

.pH?O t*.t>lo: s.th T¢inperai;ute;?

'\Sc* \ oc; , Average transftir v{)lUme: *,OjhnL Cou)1ty; HamiltOJi Outfall: 101, * ***.toinnleitis: . Each for: 2 minute$ t:0:remove

"Patho.e:enie r.ltterl'erences

.. . ."f/tt$i

Ratidorilizing teml:>late: , 'Tc-f IJU(Q.. . ,

I '""\ t. -*

Ailei11ia,C1tM:number.

l CHM882* *})IJifngin/ornltlffemfor . detenN.11tlit1on: . * * :Date /Time m/oven:

lnitiafiov¢J!.tim'1Pera.l::ilm::

' d\a> :\o.ort Daily feeding a11d renttw<<l info.rmation:.

Dny D.ate feedi*g -Time 0 08-02-16 Os.-0.3-16 2 08*04-16 3 4 08*06-\6 5 08-07-16 6 08-08-16 7 Co111ro/.i11formatioit.:

U\Jf % Mortalitv:

Averag.e weight per itiitial.iarvae::

Average weight per si.irViving l?.,rvae:

Page 26 of90 Jniti:ition1 rencw:il, pr* . teniJinati'o.n nuirillers *. Masw bat:ch ,,.......

__

I Qntfa11'.l0l . I h!ta.ke . . \.1o ottlt-D?>

. .

lt1t'*Aa*11, A . .,ok*fn.-c1c,A bk4n.*L(Dl

,.

'l'

/"" * ..... **,

_:i;,;.:,::_.,,,., ... << 7.,;0ay:icso 1:1 NOEc*.

1 *t.O"Et'::

'riv LJ-OJ.,14 Page2of7 * . C.Uent: TVAI SOOuoyah .Nuclear .. Piant, Outfall l>ate: 08-02-16 StU'J!iilitl trnd Gtowtlz Data CQNTROL. 10.7% 21.4% ,;/\: ... 1\ c I) E F G. H l. .J K L w 10 10 w to 10 10 10 IO 10 10 IO ,,,, ft): (0 {(j 10 to l*D lO to {Q {0 10 /{) lb /f) to ro LP CQ' lo <o f O 10 !{) 10 !,., .. *** 2: . ......

tc, llJ lb 10* 'l() J.() JD 10 10 10 It) ]Q *lo Jb ro la 16 lb ttl IC /() /0 10 JO* . t D

Hl " JO JO .. lo* f 0 hJ ID f u 10 10 ' ..... :. _;. .::. to l*D ro lO* IO Jt>. 10 l:b lO lD ro IQ ,,, IQ ro re ID . to .. IC lb ID JQ /0 /Q I'() C =Larvae weight (ing}.::i B-A. Hand calculated" : 5,.;.'S. c{,tz.. S..tlt '{:ti' Cl.it S.c.tC\ S. '.'2:l "i.'-t'\ wr,yst: ...... _____

--.--... _ ....... .

'

Co'mment tC>i:fd: c = crew-. d = d,ead,. rg*7 k =

m '."". miSslng; .sk ;=

Sill =*unusually 5mall, lg=> uruiSUalJY large, d&r = decat)ted and retumed,,w=

wounded, . ., . Page 27 of90 *SOPAT20-ExhibitAT20.3,revision 11-01-14 Species: Pimeohales pronz:e/as

_ _ Client: TVA I Seguovah Nuclear Plant_ OutfaU101.

Gi'owl/J.

Datil-8S.1io/o p --*a *s T lQ 10 10 to lO 10 10 [{) to tef 10 10* ro .;. ,, l iJOO% ,a*-V: \\f< X-IO 10 to 10 lCl lO ro ID 2 (:" -lD . l.0 _ f"_ lO ( y :*: t:) fQ*. 3 4 6 7 A= Pan weight(mgL

._. i_{' _ Tray color code:: "\=1/ .. ,,... 0 Analyst: ll'w"

Date: * .2;.n JV . . C = Larvn_e eight (mg)= B ..._A Hand Analyst: *" Weight per initial numberoflarvac(tlig)

= CI Initial number oflar.vac Hand c:dculated.

iA Analyst: 6\ Average weight per Percent initial number of from eontroHrci) larvae (mg) t 0---10 lO '10, \('.) "\ lO. (i) 0) .. 't LQ. *Pt 10* .1:0 .. IO . . . * /O : I 0 * -l Q ( 0 IC -Hl . ..

llf* . . -:* *_ . '* *: '\t10 159)

N32-1$,RO:

Qt.ls-. llo-0. IS-.'A--l1P,£t . . . * . . . . . . : ..* ** -o-* .cs:*:.

Comment codes; c = cleat, d;:

fg;::;

k= lcilled, m ..: rniSsingf.sk

=-

sm-;:;

small, lg= unusually im-ge, d&n= deca$ted and w= wotinded. . Commeizts.:

Page2Bof90 Page4 of7 . Pinzephtdes

pronl'etas . Crteit: 'TvA/Sequo.yah NuclearPfant,.Outranlot, UV*trHtecf*

Date: 08-02-16 .1()0% Intake 2.' 3 *4 5 .A= Pan weight{m*,.. ,..,,,,.............., . T!";iy colono<Je:: . ...._. ...:;;* ,.="'--'...;;.;...._;,.;....;;:_. . Analyst:

Date: _ ..

B ='Pan+ L111*\'ae Analyst: . 1114 . . .

Date:. .

.Hand .calculated.

Analyst; _________

_._ Weight per inltialnumber or lal'.Vae{mg).

y 10 r.* ... o (Q /Q ta Jo 10 10 z u 10 lO (0 lO lb ID re .. *. IO to ., lo ,,() to tO ' iO lb BB cc QD .&E l{l lQ IO 10 tp lO IC. IP 10 ro* (Q.' 10 ro IC lC 10 10 to f 0 lo 10 JC JO 16 IO U.) 10 ro. '\' 10 io !O =c /Initial numherofllimi.e

o * . . 'I ' .*.L<;*.*

.. ' .)t*fk> .* .

i.,;9. '>"'"**. Hand calcu. la*....... .d1**:*_*. . . . L.: u;u * . k*. ' . "'"* ...

Analyst: ------...,.....,.--. tf' ti \I 6" u* v ;Average .\veig1tt:per initial numbcr'of from ci:mt:rol

(%) l:lrvae{m ) . (,.1.7 .. "FF lO to f (j

'c =clear, d= dead, =fungus; k = kiHed;* rn =missing, .sk =sick;; sm = unusually lg= unusually'large, d&r= de<:anted and reromed, w =

SOP AT20-Exhibit AT20.3, revision ll-01-14 Environmental Testing Solutions; Inc. -t'C **no*** t\lotl*ft1'U"d llt Ff ,\ *CblllrOI, *R l*V*ir<nltd c I) ,.; 10,?% I' :c* 11* ... 2M'lli J K I. M 421!1% N* 0 .'I' n R -s T u** 100% \' *w-* X. I'

'Z . ,\A-.Hll *o..Hntll't MSP l'MSD1 hii*i<*1_

!lun.ttttt't lltSP rnt1Wl\Yl'llil"t.ftf*

10 10 10' IU 10 IQ *.1q -IO *11) IO I() to -Ill IO . *IU. JO 10 to -10* 10 'Jtl rn* JO lU *10 10 '10. IU 10 10 .JO" .'Ill JO IO .rn. IO to IO-to *IO. -10 *10 **10. IO 10. --Hr JO ,u 10 Ill 10 10 IQ*. *10 10 .9 w* JO 10 10.* 1(1 10 JQ. .9 15 .. 14 14.114 lf\.$7 16.0l** 14.'JO*

IS.82 lH? HAS -J(i.70 14.92 JG.I&. ----* .. u.oo '1669 16-.02 15.88 14.70' -IS.SO

-'IS;45-*16:13 l.1f!7._-

IS:76-.. ,, . ,,,,, IS. 15.8 . M$Q:* Q.OilU!l l!MSI>*' II.Ii. O.Ol!l-I 9,3. TVA I Sequoyah Nuclear Plant, Outfall IOI August 02-09, 2016 Chronic Whole EfOucnt Toxicity Test Method 1000.0 Quality Corftrol Vcr.ificnti<in of Onto E11t1'Y, Cnlculallons, and S_t11_tistlcal Aimlrscs No111>1 Conmlionco

'Ž""'""n1, l1111!m*l l;ohn1n101y QC n ... t'11q + l.u.f\.'4t udl:lil,(111f;il lnl"l'IW tmi::l \\'t'lgttt I .liu.l'\'l:-lm:. "dJihf I .. 'i\ uMmturorflif1-*:tt;(rt1:.tl.

ll.ll S.87 t!.SM7 20:19 -*SS o.m I 20.Jl 0-11.l(I-0.548 l<l..17 _0*178 '2<1.ll *US* 0,488-2tll UI'.

11504* lU,08 5,16 ..21.67 S,4!l" 20:87' 5.27* .ll.l*I .

0:468 20.ll . 4-1()

M4!.J 19 SI 4:81 OASI .. lD,5!).. $ . .1)9 Q;5!4 bl' H7 .. 0,517, 5.38 *OSlS -*-4.7lf (}.470" -19.l!J. *4,9'} -o.:l?<>> MU . 20.JO 4.ll OAll ll.499 20.19 4:66* Q,.f6(i m o.so2.

-11.$)4 ,O,H7, .o.479 .u,s11 0,495 _ o,srr Milllriunq_

si.wi(r...,.,p1rr-IJ.6 it.6 .7.:f 9:u 4.9 7.7 "*'

lt1.llb.l1Wml.*cr CJno1."'11tcni.ur f),5$7 O.lSS M2S

--* O.S6S 0-436--OA61 . U.SIG--VA6S-0.430 M49 .. .MRI O.SO\> t::I:= *0.*199 . U.4H OA49 0.46fl* -* Q.SOl----* .. u34 0.50! 0.479 O.Sll -0;\9' 0,461) Mt;11n,11n*l\'#l 100.0 100.u IGM 100,u ICQ.O g'/;$* 9M Proltt1

_____________

1_16_l'I_

ttllt1 ..

0;$Zl ti'518 O.SU4 0$14 (1;;161 .. Q.501 o,136. Qief11rlr:trtlf

... l!J.'U

-"" -ll.6 8.6-1,$* 9.0' 4;5 .6;l '5.5 *u l"Tml\1 ml!.1.Uio:a frt1ltt -Wl\flt.f t,;\j Nol Nolojlpll..,bl*-

l.& 9;7 Q;9 10.8.

G;1 Mioimfl!li Sii!rll!l..,it Dffl'oie..ccr

llMSll h n-u!liorlesr prid!ion, Th: PMSD_

l'dte<lll ti<iliwen co_niiol iUlil "'""°1"'1 IMI tM be 1ilinlll1:1111 ll!l!icil1 i.st l:CW<t b0u1id d.1tri11lni.'il by .USill!A (lofu pmc<nl!O)

-

bound by USf!PA(90ib

"'JilW Lo.vctlllld UP,pcr PMSD .Wi*ililorittini:d llom nn m1afrom

\\11rrrri1i:iioti!lrl!l<X)'VniillliilfjY lCiOlb). lJSEPAc lfJOlb, 1'01o! lt*Pliit!

_lotoil.iboi;i1uiy ofi!PA Shon*l<<m Climniond AcvwWholk ll{lj...,1Toii10Jly1'"1

/\foll*Olls-.Wlun;.,.

I

..

US l'!'ll>'.<*i1>1*1\Utl1C)l.,C:ihl:ion1111;Dll, TV A t"fu¢1ear flaJ1i 1 OutfD:lMOl . .

2016 Stati$tical Analyses GroWth 16" Eni.foaJe;:

16 Test:!µ: .. f'pFR9R .. . Sample !O: L\a:ll.IOj TVAJ SQN0utfall101 DM!i-Olsciiar91:-

Mo71it0ri119 Report pf'.pi1nej:ma1es promeias Aug!,fst 2o1 s *CommerilSf o.sir;;o o.5550* P<mtrol'-OV o.s!>So 0.48.20 .t-9*1. !l.4880 G'.461.!l 21A o.s270

f!,4650 42;8 0;4S'itf
i)5,5 . O..f70'i:r lM!lSO .1ilil o.:ii$6o o:S026 * . 1,{47sq .dono,.o,s . Corilfol-JJV . i:>otnt !COS. 1¢10 IC15 *lfud teas Ll.':41) IC&l *j0}1 2i.4 42:8 'e&.6 100 .Mitan N-Meari 0.5225 :1.0082' o;s1a3: 1'.0000 0.5035 O.W1S i>;457s Q;902S 1)£130 0;9913 0.11919* Ct5008. Q.$!>52 DA!iss
'% so* 20.06?' . >100 >fOO '>'100 >100 >ioo :>100. P\'(ltcleQl:
TestSf?etjes; l).5250 0.4230. :0;54811 o.47ao* n.s1 ** <>:s4. *tiAaoo; a.astir o.;52ao :f}.4319 '-0;44\'lil*
n,s340.. o .* ooto* ;0,,495,0.
OAtlllO .... Tmnsform:*tJntranstormed Moan Miil' Max .. C\1%
0:4230. 0.5870 13.589 0.S183' 0:4780 0.5G50 d.5035. p;4610 0;?-490 7:4\'.lS 0.4300 o:s:tro s;sa'i o,s1.sEr o;s:i1o 4,819 M310 M9!'10 ;;:aas o.5'i.ios 0.4660 . q.5340 5;547 oAass .o:.c1()
o:s110 4.491 N 4 4 4 4 4 4 4 4 0;,941.98 1"52143 :0:10128 MSOu 0.05997 ...... -* 1-Taifed t-Si:Jt. *eritical 0;593 2.:410 2;030 2Ato o.1a1 2.410 i251' :Z.410 oJ*oa
a,it1cal o;aS4 :ts.oses*
... 2.4469'1 MSO o;osoo o.osoc:r o:oeoo O.!JijOO C;(lj'IO!'l.
lsotoQie*
Moan. . H*Mean' D:51Ss 1.0000 .<i.5035 o.971s 0,4908 0:4908 0.9469 0;4Sl5 0.!}291 0:4815 0:26917 -1.!)903 MSD MSB MSE f'-Prob <ff o.nsn o.0022s. 0,00124 o.1ao4s s. 1a 1.lnoar lnterpolotlon wt..1::4EXp! . . SlioW. . . . t.o -r------------1 0.9 0.8 O:'l :o.s: c . . g_ o,s &b,4 *0.3 D.2 0.1 * . .*. ,.
150 *0.7 . ...---------------------*
31 ..* f . . q* . l:l"oii l:I . ;* .... Q;i' 0.1 Page 31 of90 of
",, .. ,,._ .. **:.>:*, <:> }
Tostlm; Sotin:.,1nc; TVA I f!lant, OutfaJI 101 -. ..,09, *20Ui SWistical.
f\nalyses Larva1*Eish' Gt.oWth* and Survival.
Te5t4*Qay Growth. Start Date: 8121201.6 Test 10: PpFROR . Sample lot:; "NA /<$QN Outfall '101 . .,; Intake . Lab 10:
.. Testlng Sot.
Type:
Report End Date: 8/9/2016 Sample Date: August2016 Comments:
PretocoJ:
'.FWCHR-EPA"821
.. a-02.-01a promelcis Cone-% Control-NON 10.7 21.4 42.8 85.S 100 !ntake Cone-% Control-NON Control-UV 10.7 21.4 42.8 85.6' 100 Intake. 1 0.5870 0.5650 O.S2.7Q OA810 0.4700 o.4660 0.4790 2 .o;ssso 0.4820 0.4610 o.soso 0'499!1 0,5020 O.SHO 1'illean N-Mean o.e22s 1.ooe2 0,5183 1.0000 o;so3s o.971 s 0.4675 0.9026 0.5138 0.991'3. 0.4623 0.8919 0.5008 0.9662 0.4863 0.9383 .3.' 4 ll52SO * . 0;423Q 0.5480 0.4(80 0.516o O.S4!10 0..4300 0'4490 p,5:m> o.52ao 0.44!}p ci *.
Q;5Q1o. tt495,0 bAS,oo Transform!
Mean 0;5?ZS 0;5183 W?l:i3S 0,4578 0,51$8 OA¢23 0.5008 o.48ss Mlit * .Max. . 0;5870
0.56$0 OA6.10 0.54$0 o.;4300 .as270 oAa1o q;s37p 0.431.0 Q.4990 oAseo o.5340 0,460\}
13.5.89 8,633 7.496 *8:981 4.819 5,547 4.491 4 4,. 4 4 4 4 4 4
.. t..Stat CrlUcal *MSD. Auxiliary Te$ts . Sfati$tic Critical.*
Shapiro-Wilk's Testrooiciares normal (p* >. 0,01)
Skew !<.Utt F-Test indicates equal variances
{p = 0.27:) 4.19692: *47:4683 n-.;ot;;;
..
... * :;,;..e:::;n.:,;;.t.,.,*(ei;...'.-iii:.'""'0.,.;9;.;;:2..,l-""'
... __,....,.'""""-.....-o:;;.""1b;:;,.
1;;,::29=-*
,_,,,,.,...,,, __ 2"";""4""'469;:,;*.*.*;;;..;1'-*
--==,,,..___,,,...,,...-..,,.....,....-=-_, .. , Hypothesis TtiSt {1-tail;0.05) . ' . . . . . . MSDif. MSO.I? .. MSS M$5 F..'pi'.i)b df Homoscedastic tTest h1dfcates no o;04837 Oi09333 0.0"0205 1;LQ.O;J24'
0.21599 1, :S Treatmel'Jts vs Control-UV
.. / \).7 M Ji;; o,s. ... :t e GA (!) *:>. Ill 0:3, c t.. 0 .. 2 0.1 0 :z 0 :ii: 0 .;, c: 0 *O Page 32 of90 > *=t g e .. 0 0 6.05 lelll?l .. of slgi'!.ifrcar:u::e 0 .o
. Species: , Plmeplurles pfomelas *client: TVAIS¢guoyah
Nuclear Plant, Outfall.JOt,,UV.;treated.
Dail
Cl1f!i1ilst
Concentmtion:
CONTROL ov::trea(ea:
i0.7%. 42.8% 85.6% ti,Mf%* Intake ... Hard1Jess
, (m" CaCOJill Condui:tivity
.(µmhOs/cni}
A:llmlinity . CaC03/L) **aardnei;s . .Cm . CaCOJ/L} *Tern ern:ture,{"C)
Page5 of7 Date:
measuremenls onl *.) 2 *Temperatures perfonnCd at .the rcnC\"l'l ortenninatfoo by the*aaalyst.iderttifie<l iMhe paily Renewal lnfo.rmalion table loea!ed on Page l. **d._lka
ity 1 total ll:sidual chl'orlrte performed' by the analyst idei,dfied on the beqch sbeetspecifie for each analysis and 1ran5cribcd to this bench sheet .... . * .
ToJat residual i:llforlile
WllS performed on lion"ttcaled:Ouefall I 0 !. arid in lake sample$; Page33 ot90 SOP AT20 -Exhibit AT20.3, revision 11-01-14 tration Species: Pimeol:ales promelas Client: TVA I Se uovan Nuclear Plant Outfall JOI UV-treated Parameter CONTROL UV-treated J0.7% 21.4% 42.8% 85.6% 100% 100% bi take Conductivity
(µmhos/Cm)
Alkalinity (mg CaCO;/t.}
H,u*dncss (mo CaCO;IL} "TR chrorlne (mg/L) *Tem erati.11'.'e
ec) H {S;U.) DO(m 0 Ll Conductivity
(µml1osfcm)
Alkalinif;y (m°CaCOs/L}
Hardnt:$S 1mo caco;1L> *TR chlorine {mg/L) Page6*of7
. Date: *
, 0** eratures perfomred at the time oftcstiniiiation.
renewal or ienrih,1ation by lhe anruysr idenlifi.ed iii Renemu.lllformatien l6ca!i;d on Page.I.
y' hardness and. total residual chlorine performed by .lhe analyst identified on tiie bench t0r eacll an:alygls mi4 sheet . Total l'C$idual chlorine*
WI!!!
on non4reated'.Outfalll0!
and Jiltairesamj>fes. . . . . ' .. . SOP AT2. Q.,.. Exhibit AT.i0,3, revisiQn ll-0 H 4 . '
¥ ...... , ** .. v-*><* '"<"* "'*****,..
)*t* Iii ..... Page 7 of7 Species:
prorneJas . . . Client: TVA /Sequoyah Nu:elear Plant, Outfall lOl, Date: 08-02-16
Concenttation Con trot Non-treated Concen,,.
.*
tratfon: Control* DO:( !i/L)" *. Non-.treatci:I CoruluC.iMty
(µrn11os/cm) "Mkaliniti*
Jnig
_AJialy5f . (Anal st identified.fur each day; erfOnned.
H. DJ). *and. eonductM measurements only.) ] 2 I Initial Initial Final Day (Anaiysndcntlfi!id f¢t eacil day. ifonni:d R.IlO. ilnd*coildilcuvit
measuremcnlS only.)
renewal*i>r ierminatfoncbyUie analyst: identified in the Daily Renewa1 I11!0m1aiion table located on Page l. Aikaliility; bm"driesS:tli!d
!Qlal re:sfdual perfomied ana!ysi ideritm'ed on the bemib sI1eetspecific roreach anal}'Sls and transcribed 10 this liench sheet by: . . Page 35 of90 SOPATfO-Exhibit AT20.3, revision 11-01-14 TVA I Sequ()yah Nuclear Plant, Outfall 101, UV-treated August 02-09, 2016 Plmepllllfes promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method IOOO.O ,. L'.J .) .11nvltonfllel\lill SoluUons, Inc. Daily Chemical Analyses 1'111jcct number: _____ 1_163_7 Conccnt i'nllon Pa r11n1ctu l)nv Q Duvl Dnv.2 ... DuvJ .Dnv.4.. . Davs D11v6 Initial Final. .lnllllil Finill
lnllhtl Flil11I h1ltinl 'Finltl Initial Ffi1ill lnltiitl Fl1111l . lnltllll Finni lnH.tSU\ 8.09 7.82 7.88 8.06 7.80 '. 7.76 7.99 1,78' '7;73 7.116 DO rn111/Ll 7.8 7.8 7.S U 7.8 7JI 7.8 6A 7.8 7.S .7.7 7JI 7c8 7.9 Con1r 4 1, Condnctlvlh**<umhoslcli1)
)Oii ;,o;' . .. .318: ::*:,, *
318'.. 320ii.*'*'
Non*lrtllltd.
AlknUnlty(m!VLCoCO>l 59 ./'*,;;j,;:;*::;
>*.

,:., ,::*
,

: S9 : .. *. ****'* *' :.**.::-*, :* ' H11i'dJic!S(mglt.CaCOl) 811 '..'.""*
..
';;::*'* ;;;; ,, 9(i* ...... *,'::.::::*:;T"''"*
90 .,.,. .:' *:.* '\ ;' "'
ConductMfytumllos/lllti\
.*301 : ::,;;: *<""' ;ns ;:1;::'"-'*'s:;c:;:
312 " .... '322 "'.'!!>'"i."*'
!!OS".
311 : ::*:*>'0/*.:
.. 317 '" * ,,,, .. , ..
Alkuliii11y,(11iglLCnCOJ)
.:59
........
2i;;j

,*c:.1 . *59 ,; .
.. :Ao,) .. : ....... ,, *'***'**'e}.* .ss
... *:*;,,,,, ,;: ... ;,,,,.L::.,;'i,,..
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.oo ... .. ,., *,:'***
.. *":'" '*" :**" .. " *sa :>:>:**< .... ,***: '*:* .*:-. .,, .... , .* ** .,.* .. * !lU .** :::. <':":.'.
" * :,. ;;; . >;:'.":'.':':
.*. *
.* ;o:*1 > .,:./ * . 'fcmncrnture i"Ci 24;l! 24.5 24.7 . 14.5 24.7 24.6 ' .. 24.7 1111 tSW 8.04 ' 7:84 ' . 1.91 7.88 . 1.12 1;!14 7.49 8.02 7.85 00.Jlll"IL\
7.!l 7.9 7.9 . Coniluctivltv rumhos/cmi
3.1f *'" j4;7 24.8 24.s 24 .. 7 . olUSUl 8;04 *7;84 ' 7.!)2 1.11 1.88 '7;74 7;94 .1.sa 8.00 7.74 7:84 7.85 1;ss : DO 1111u/L\ Vl .*
1,8 1.8 7;!l . 7.8
7,fJ .. 1.ll :M 1.8 1,9 CanductMtv CumhlillfCUil*
28.S .,. . 2!14 ., .. " ws .,;,o,:,, * .. ,, " ' :298 :.*:,,,:;_,, *, ... 289 ..... ',/*.**:c JOO.** :s,01 1.Bii 8.t.l 7:9. 8.0 '. 211 ... , .... , .. . >'71.9. !/,9 'Vil . . . . 1.9 .. 7.ll ?,9 . .7.9 'S.S 'M :7.1 UJ SJ M Co'1iiluctMty{ullilto$/en1\, .2:u J .*... , ,.;.:.:;>
227 ,,,,,,,.,.
.,, 216,
,, : 226 223 :: 0:7,*'."";<
' 224 l ... ,.,, : .:7.: "233 ... File: $qri1111,Jl8Q211lcheril:xlsie Entered by: J. Sumner Reviewed by: -d(-
1 **1 *-1 *1 l -1
1 I .. l. . J _J f J 1 Page i of7 *.($11uuc.Wio1ecEtfiuentT¢riclti
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Client:.
Facility: .
TN0026450'. . . *
... "
l 1)70 I
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time . MH'sw *l.ffltch.t1$ed . o r og"f12 ... 16 : j, .
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's .. I, It!;'. 6: . . I
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.: .001. '1()01. %.Mortality,;, ,, . .. aJJ '01.: . %CV;* .....
'4.'11. . * .Snmpie num'bers used Ouffalt'.101 I Intake Analyst $1lrh,11ll:UJi
<!/test endpoints:
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!Cu >u:io1.
Page 37 of 90 SOP A Tl ATI L2, revision l l-01 ... l 4
! .J .! / . Ceriodaolmia duhiit Client: TVA /
Nudear Plant, .Outran 101 CONTROL-1 . . 8..ilrvival JJ,e.pruef11<:tio11
P/Jfi! .
Day 5 6 ., 10 Young prOduecd Adiilt morratitY
*
Z. Youngprod11ted
Adult inoriallty J
Y<iuqg Adttlt inort:ilii:y
f 6 \'ming producci!' . Adult
7 Yi>,ung pr9iluccd Total young produced Xfor3"' Broods . Co11cen1rali.01fr:
.. CONC: 10.7% 1
M '0 .. a c f'r (3 . *n .*. '(\ .. 0 .. 0 Aaliltinorrauty L.. :*L.... . L L:*
C ** ** .*L '. ****** L *** C L
L. 2 Young produced*
r\ ... i"\. A *.* (j' . *C). '. .' O.*' ' 0 0 *y 6.:; : 0. Aauit mortaliij
* \.: : L * * : '\,._.;, c * * '\.:.....
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L
5 Yonngprot)uc1:tr . tQ . .. t1 f'1-.. tt.: ,,.__ l 6 . H . ]l .. Adult m0rtality
\_ \..;_.
L . \....:. ..**\.;...
L,.. l,..
i'L 6 . You1,1,g produced :* *o o* (J. . {\. .. .. . ' 0 *. 0 . .0 . A . ti ,.\dultmorii!lil}'
.' \.,-. \...._.*.
L.* *L,...* '-,.;' \,...;,.;*
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'.3\ 41;, ..
' *: 4t> .. 3o'$ l S j'
3!. FinalAdult.Mol'ttlllty. \....;...'
L * ..... \;...;.. L.. L '.L: . L'.' 'L . "L :*: "-.
.\i!ul1.mcrta!ily
"'" oV<t(o""""'il.c:inied
* : Concenirailott;.
. '%.MOrialiry;
* ** *
01. IWelip

1.._* ,ra;Retludio.n fromaontri:l.1'"1:
*** ...,$."t.7;;.
Page3Bof90 I I l I .J _J Page 3 of7 D!iloiliiD!mia .tliihia . .
-rv°A1 segyoyah'NuctearPlknt, Outran 101 (:ONC: .21;;4%* .S11tvivui
<<nil Reptod11ctio1z Data I)ate:
Rti:iftcafenumber . mY:*
1. . . . : 2 . 4. . 6 .
7* s 9 10 t*
N C::,
0
0 l\ 6.
0 0 0 * "L . L .. *\..... .L. . t_. \........ . L...
L. L. L ':Young*priJd\r(:cf) .
0 . . *o () ' 0 . u 0 .. 0 0 ('t n .Adtilf t.._. . .k, '-L L.. . L.. . \....; L . .. ..L r)* *O* :{). n o*. 0: .. c 0 D 0 ,i\d'illtmortaUtY
' .*. \...:_, . x,..... .* . \_.., .. * *'\,\_ . . L . :. L *. *L.....
L,. '--.4 Y!un&pfC1duc¢ti*
.. *. *'-\ ..
C::.., 5 * ""\., **"{. : S. .*;
\.... * \.-* L \..-L .\...... :: L L. . L. '-*
s Y0uilgproducc:il*
.. .\0 \ "L.* 1:"1;.. \ 1.* \":I.\ .; \ 0 \,_ . 14 l\ Atlldtlli{>i:tilllty '\.... . . '\,._. L. c '\;__ L '--'-6 D.. .D. o* .(')' 0 0 c *o 0 0 A.:duJ(nmr,tnlity
*** \:....... . L . L _, L \.,..;,. "--: \........
* . "'--\.._ * *1 Youngpro,auccd
l"l . \.\. l <<;.. .\"\
.\ -s . l *S . : °!>\ .
' ?>>1.. '?:.:\.\
$lo ?)u o\
a\ Fiil:ilAdwt:A>lorlality
* .. '\..-*\-. * '--*"---. \......--.* '°'-'** :\.......
. \,,;,,,:.
' \._ * '--
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%Mortality:
01 .. .%Redtictfon from Control*I: -s.eo 1. 8ff!Vh>ala,1d Reprod11ctio11 Data .9 .IO 1. :v-Ouagpz.'OdJicecl n: .. 0 .... o.. n 0 . **.o 0 a u 0 * ..
L L * 'L. L LL* , *L . L... L L ('\ 0 L .(*'\ ("j .L '-... S .. '-'\ L \.... t"l 0 '-\.__ l"\ 3""\ 3b '--Nf!/tf< SlJ"'!plil brn<iif<sinsl=brcod CO"'cmv<J11u(o11tmint<c:oniod 0vcrwl1fuduh dwlM . . * % M0rtality:*01. Mean OffSpringlf'.emale:-s.c 1. Page39of90 SOP-:Atl l-.8xlllbitATIL2, revision 11-01-14 '-. l l l I f I J 'f Species: CeriodnpJmia t111bia Client:*. TVA I Seguoyab Nuclear Plant, Outfall Hll os;;oz.16 CoNc: 85.6% ** * * * * * * *Surviv.al and R.ept1Jd11cti01z Ditto Day 3 .... 6 Finni Adult 1\-JortaiitY\;.;._' . **'"-'* .'. . .'\...,;.;.ll'Olri: AdUl! (L D "delid). split .CQ "i2!i Carn.:d owuw&* * * * * *** * , * *. * *. ' __ i\'iean'l CONQ 100% 811ritlvtil mzdll.eproduction i>a_ta; Day 1 Young pi:o,duccd. iiwrtnlity, .. \....: .. L 'L*. L L * .. *\;* '*,, t \. ...... c* .**L . 2 Y.riung produce1f -. . . p.., * . Cl' **.*. * ,/S:1"\ Ci 0 ... **; *.** 0 . 0: .. , .... :C\ . O*mortlillly; 3 Yo.1mgAdult morfality.. 4 .. Young produced Aduit mor:tali!Y 5 You,qg: prol.lilcc:d Adult mortality 6 i'oung.pnuluccd', .o :*** o **o .. a .. ("). f!\*: o .. 'f) _ n ti* Adult mortnlJty 7 Young produced. Final Adult* J\>lortality NDk: Mot1 il!onofijy(lo* live. tf dead}.*SB.f'Pi! !!'!"id d;im CO="""* iffi:r'i'O,..,.;"" c¥1\'c40¥0r\\11lniilllfl lfllrill1tir.DW"1);CiJ!li;enir..a(ill1t: .. 01.* '2i*:">I ....... ... Page40of90 1 I i .. I . I _] J _J ..J.. &rtgdni!tnia ililoia .,. . .cfien"(i TV ATSeq\tQwh Nuclellr. i>laitt. outrarJ lot .* 08-02-16 ¢QNTRQL;:.2Sili'Vivtil a/ill Reprotl1ietitiizD<<tii RepUcate mnnher * , 'L :L ... L. ... "\...... .L ..L. l.-.. L.. . L L .' *O* .. '()** ' o. ' (") . . f:'l ...... : .... D* ... :('\ . ' L} 0. -... * . *\_-i \;,,.; : '-* L . ' \...,;.., \..... . . .* L* : \........ '--\...... :4 prod!tceq.-:as **__ ., : _ ?;.. t _ .. ' ""-' :S : CS :.. : . \...... \;.,..\,. \.:... * *\..._ "L. L '-'--.6* * (j* *Q . O .0 . 6. b o O 0... L,__.: \..... "L. 'L:,_; : : \...... , \,__ '--\.__ *:7* -*tio 1t )"'\. .lq, . : X1. * \'1 \'5 . *; . "'i . "'1.C\ . ' 0 \ . "14-:.. \ . 0 ** : Ji'.iiiat.AwltNiortali& .. N'tlk:*,l\illllllllOmli!Y.J (J..*"'!Wc;P"'<k<lcl).SB ..Com:entraiimi:.. 8tiivfval artdReproili1i:lion.1>ata RcoJicatenum6cr .*1. .f). 0 .. 0 0-.. 0 .* 0 . 0 : n 0 ' ,\dult mortality** .. \;.' . L .. L.... L.. . L. . : \_,._:: . \;..,..;., ** L . . \..._ 2 * ** * .)'oung.tiraduced I'l * .. -(\ f1 .. * .. CY * (). .el 0 . . . : L _ ....... L.. :L.. . L .. : .. \:.....;.. L., L. . .. L . L 3 .-'.r') £1. Q.0 : *o * .Q ('\ . ti: .("'\.. l "". L .. L' .. .. \.,...;; .. . \-L . L .\_.... .4 \"oungpr-0d9ccd ,c:;. . *** S -l .S i <:"-\, .\,p \p : ... J,:.: ... : t L, : . L .. *c. \....;, L. . L *L L .. S "'. i'Oul1g J;f:) ; . J,C t"\* I "L, i .. \ 1..... l°4 .. .. tl ..... pa,. \. "'t. .. , L-4. \.-.. ** \......:... : i. \..._;L '-.. '----6 V:-Ollt!g produeeil:n : . . O . . , (') .. L r:-i. . h .ts 1'. . _()_:c :. \:,.... '\...._.:\.....;. L .. > 'L . ..L.. *\........ \...,.. . Mire ss .. bc1we<n im>cla,y$i. Co*tanv Ovi:r-.<orr,.,,;M,,.nicd4'-.r"illillllult IQmfcrl. Ct>11eentrnJio11: .o .\ A L '- L i1-\,._ . %07. Mean Offspring/Female:%Redueinon from .. \'1. Page41 of90 SOPATl'l --ExhibitATlL2, revision ll-01-i4' Duy J 2 3 I* O* il :0 .:l 0 ,() * : :o. J' o* 0 0 4 *$ 5 4," .en *;;. .10 *ro: 13 6 *o**** *0 *O ' 7 16 l6 14, 1'111111 31 31* *31 ilny *: .I . L . J 1 O* .. *Q*. (l. fl* I) *:o J "(}" JV *o + .. *5:.: 4* s: .. 'S JO" H'* ii ., ti" 0 \l. '1 A6 u** lfi'll" 32." 32 *Z:i.4% .. " .. ; *Diiy J _l_ 3, *.1 CO'*' o* *u ***-.:r ;*o;-*O* 0 .:'f *o. 0'.' 0 4 .)' :.4 ;S $ . J3' . JO .. .. I:? .. .6 .... '.'(J. '..dk O'. .7 '.'14* * .. ts* IS *T0111r ar .. *n 31.' li11y r *z ., J. J 0 !f .0 2 .. o:* i,IJ.' ;'0 *3 ... :'()* .. *o,. **IJ ..........!* .. 5 .A. s 5 ll* *'Iii' '12" **:(!' .. _ *o; '(}: 0. ,, .... '18. **1x. ':18 -Tlillll ;r4; ,35* 35*4 $ Ii .7 il 0 *tt *o .o (). "():' l) () 0 0 0 d . s 4 12 ll* H* *11v (l O* '(} Q .*18 I:>. 14 17' TV A I Sequoyah Nuclear Plant, Outfall I 0 I August 02*09, 2016 Verification of Cerio1/apl111ifl Rei>roductlon Totals 856% .. *a 9. IU 1'olol Uny I 2 3. o.*. 0 0 0 "I 0 0 *o. 0 0 ll 0 2 o*** ll "O. o. 0 *a .. *a 3 0 0 '() 3 *I if 43 *4 l s .. *s, 10* 12 l{j:' -Ill .!I 14 12 , .... 0 () .. o. {! 6: o.' o. 0 IS u 15 153* ., .. 1s* IS II!' *32, *30 JJ , , '23* 29. 306' * .'flilut:.*3% .. Jl' Jt.inlle.iui ntllllll*"'I s 6 1 0 0 0 0 "() 0 0 0 0 0 () () ll s s 'S"'. i3 12. *12-" II' 0 0. o' o* 19. 11 19. .t;t.' 3G :;u36' *30: .R '1li<*tcni1mtmr*'t"olol ....... ... A 6 . 1 , f!. .. 10 *)* l. .. 3 -..!: . ,5 6 .7 *o . : .. o,*. *il *O O* O* :if:: ;(I .. "I *O,i 0 .:o 11'*; O* O* 0 . *o *O (l {I* (i'.: (l () 0 2 :o' .... o .. . *o 0 0 "O .0 . o. . 0*. o. -0 (); o. ,il <o: *3 o:;: *IJ' *u l.l*. *o I.I: l). .:4 4 .4. .'S . : .. 3 *. s:. . ."\' .. .:43 .. . *4 ..ii :s *:5: .. .o** .. 4 $ !LFi .. *1:?* . . .. It: 10* :ll* U* HS :s: . iJ 1"" 10 ;n u* 'Ill* ff (j* o ... CL JJ. Al 'IL *O: .6' :o ... : o:* :a* . ::Jl .o .. *o, 11."* IS *iM 11* t:ll* *16 T7*: VI" .. *::1154 .. * ;7* is** IS'.* 19" *u ,.,. l'1 "19 . 3S d!IJ. *.33'. JS** *.w:*, 33' . *3't 31:? .Told I 11 JS J6. ':31 .'JG' '3.'i J!I CtiritroJ.:2.ltcrilleiaii! nuinboi* " 5 6 .7 . .8 9. IO 'ro1111 Rent1rn10 numtt<rr ... ;Qtiy. I. ;?. J . *4 s .6 1 *o o. O*'. 0 0 0 *n* .U. I. .. IL . 0 *11 0 *o" o. 0 0 0 .0 ... o .. .. u. .. 'ff', 0 ,z, O.' ... o .... ll 0 ... o.* 1)* :o "Q;. 0. () .. 0 ... ]), (I () () <l **o* "ii)': *u .o. 0 .o. n* ,4, *.s . '4 A. 4., ;S .. s:: 44 ... 4 *:.c_;*** 1* 5 1** . ' .., .it* $.*: .*1z. **12: 11.: lit* 1:!' ; 'J4' .. -ll 'U9:". .. s 'I()' .13'. :.ir . ::u. :*n ... .to >Hl *:o 0 0 0' :o .. D '*.O***. .. g '(j .. O< *"O' *a Q* .. o* **-o,, u IS.. IS'. J9 16* IS IS" .JS Hill. '/* :rn* 11*. *IS l\J M '1'4" :"17 ;3'4.". \1% *;l(f. .30' . ,31, ,34 : ':;J(., 373 .. ... 29\ !*33 ;:'31' , .. z9-, i"1L 111' . Ji.tto1!llforii iiwnblir' Ti)(ul 4:.,. di l'f* '7 ll* !!* 'Ill' *Udy . .:Jtif illtntc nu1nJJer*I 2 :3 4 .. 5* IL 1 ,<J 0" 0 . lL .. IJ ... it 0: (f *I 0 .Cl* .u: 0 . -0* () :.-.o . .0 0 : 0 "U O:' 0 ':O' fl' ... ... 2 **:*"** (J' *o.*: .. II *o .0 !I. -0 'O *o*: *o *:o:' o** **o. ll *Q. ... *3 0 *;0* 11. O'.** u !), II ... 6 .. 4 *S j. .S, 48 ... : .. :'S"* '$* 4 .. : .. iL 5 . ..!) .. .. .4'. .. *n *Jo 'JJ' :12 ltt: 'fl' CJ f.. 120 ... D . 33 1 l.3'4' ' tl .dt 0 0 .*14*, lti.: 15** >>lll .. 14'. HS5 'J'f.* .J2 ,*3)* ;,.11: ..JO* 333 s Ill'. *10" *.14 tt:t ' XL ... 14:. n** ' ..(}; u '.o* .il .{) *O 0 7 .'20" '.Ill. ' ll **rs: l(I. I'S 11* llfnl .. '1$.' >J3* ;)!I: ,.;;n .. . J1 . '38' 31;. 8 !) . II) TolUI *o 0 0 (), 0 0 0* il 0 () (l 0 5 , . Ii" s* 48 12 10. Jl:'. '119 .*IJ. .If IJ:.* o, 0: 14 16 . W4 J4' *.Jo. 33. 331 ... To111t 8 :9 ilO'. (Ji> "°' 0 *O 0 i) o. 0 O* .. .. 0 'j)/' .'Oi l\. '>(i 5. S:I. . JZ.: 1.2 .. *.'.13* 1!?4 *:o -0. , . '.Oi 0 *:19" 1.:1 *111: 177. 31 *33 J6 35\1 ,ll .. !1. .101 O* :ll ..* o, .o o* **o .!!'. (1 'll U* 11 .:o .s. '.L . ,I, *39 '* :.:.12.: : .. fl>.'* .. 11' . 114 , 0 O* "(}* I). H* 17 IS' 152 *'11 *3J . Jlh JO$* fl II 'JQ.* 'l'tiiul 0 0 . 0, .0 . ... *a.,* .. o .... (). . 0. '0.l .0 II:' 0 , ... '*l'i"*i .Ai.. A. *.so '.111! '.12 "I:\;' i20 '() *II-j)' ... .u ts rs* i'75 "J6 ... *3.l * .34. 345* (%} Control*i 16.7-% 2}.4°/ti 42.8% 85.6% 100% C1i1tirol*-.2 Hl0% l11t11l1e Outrnll 1!ll: DmmeWs i\*tSD v11tuc: *PMSl>: i111i1l1et l>il11neli'!i vnlue: PMSD:. 1 3'i 31 31 34 32 37 29 35 l J. JI 3j, :32 32* 32 JS .32. 34 35 33 .31 33 6.6 J.40.J 4.G* TVA I Sequoyah Nuclear Plant, OutfaJI 101 Augusto2 .. 09, 2016* Chronic' Whole Test Method l002.0) Cer.lo1lupl111Jo 1'11bi1J Chrooie'Whole Efflt1entToxicity Test* . EPA-82J..R .. oi .. 013, MetbOd QutrOtf Con,trol.Verifle3ti<Ju ofData Entry, and StatisticalAnalyses . . .. 4. 34 * 35 34 31 3(; 3l 29 32 MSD= PMSP"" .lfonli1::ltem1mbei' .5 (j 1 32 30 31' .30 33. 35 :p, 36' 30 *. 33 34. Jl ' 34 3& :w 36 35 38 <3i 28 3:! , .. ,. 37 38 32 ... Mii1ii11u1i1 Si1!1iili¢unt Pcrccnl Minhm111rSig1illicn111' Difference s '!f 18 29 Jl 31 34 32 37' 34 30 37 j3 JI 31 36 33_____ ____ 1..,,1.,..ii3_7 Sm'l;!Vlil . A,*erage rcpnuJ1111tlon ft!dnclioli fr11ni '(%)a*rl*Jioo (o/.il *lllioliiil cunll'ills (cy,,): 10 29 100 J0.6 .. 5.6 Not., .. ,. ..... 32 mo 32;2 6.0 .5;2 31 1ilil 32;3. 5,7 -5.6 j() iOO 33.3 6.3 "8;8 ... *-** .Jl IOO ,JJ;i 6.4 -8.:z 36 100 t!S.4 5.11 -15.7 30 HID 30.;; 4.9.* 1110 34;5 6.0 *13.l P,MSb*is n prccisiOlk The PMSD is lite minimum pcrcenl dtfli!rC11co hetwcc1Hlu: co111rol*nnd trcn1111cnt* 1hnl-c1111 be statistic11lly* iii a whole test .. l.owor PMS[> bound USP.PA(I0 11' pereentfloj = 13%. Upper PMSD bmmd dclermined by USBPA {90ui percenlilo) = 47%, Lowet.tmd t!ppcr PMSD'bmmds w1ire detenninud from tl1e*IOlh and 90th pcrci:ntili:i, rospeclivcly. orPMSD datn from EPA's WET lnlerlnboratory V11rlilbilily Study (USt:!PAilOOln; USEPA, 200lb), USEP/\. 2001q, 200tb. Final Report: lntcrlnboratmy Variability Sht' ol'EPA Short-term Chronic and Acute Whole Effl11e111 Toi:icity Test Methods. Volumes I and 2*Appcndix. EpA,821.B-01*004 and EPA*821-IJ--Oh005. US cnvironmuntul l'rolliclio11 Agllocy, Cinch111ali. OH. TVA I Sequoyah Nuclear .Plaut, QutaU HU A-.gust 02-09, 2016 *Statistieal Analyses .* Cedodapl!nie Survival *andReprol:locUcmTast*Reprodocllon Start Dale:. 8!212016 End Dale: ai91201.S Ti!sl'IO:CQFRCR . . Sample ID:. TVA/ SON, Outfaft 1011.ab '° .i;ts'Ett\llr, oiviR,.Discbl!rse IVlonl!ol:int!Re?or( dtib.la . Sample Date: August *2016' comments:*Cone*%. 1' Conlrol-131.ooo* Control-2 29.000 10.7 a1.ooo* 2i.4 31,000 42.8 34.ooo 85.6 .32.000 100 37.00() in lake 35.000 2 3 31;000*31:000 33.000 *. 3'1.000 32.000 " 32;000 32.000: , 32.ooo 35,000, l 35.000* 32'000 : 34;000 35.000 .. $.000 . 3S:OOO .. *4: 34;1JOO 29.000 35:ooD 34.00Q 31;oob 3G.OOO 32.00.0 :5: 6' 32.000 30,000*28.000 3MQO 33.000 *32.QOO 36.000 33.000 34.000 34.noo a&;OOO 35.000 3a-Ooo Transfoi'm:'Untr.aru;formed* Cone"% Mean. N*Mean .Moail. Min Max' Contr01*1 '30;000 *1-0033 *-so,soo.* 2s.ooo 3;;.;000* Contto1:2. 30.500 1*.0000 2!t0:DD 10.l' 1.0557* 32;20.0 21,4 32.300 1.0590 30.0,D(i **3MOO 42:8: 33.300 1.0918: 33;300* 3tt0 37.000 85.6 33.100 1,0652 33.100* 30.000 36.ooo 100 35.400 l.1607 .. .35,400 3,.ooii 3a.ooo lntake 3.4.500 1.1311 .32.000 38.000 Auxiliary TastsKolmogorov o T'*!I i(ldicates normal dislribtJl!on* (p*> 0.01} Bartletfs TesUndlcates equal variances'(p = o.99} The. control means aw not st nllican . dllferer;t . -.*o.89O!lnnett'sTest'100 >100 Trealmentsvs.control-1:C\1%. 5.597-G.001 s:ssz. 6.339 6.446 5.BSS 5.99.S TIJ 7 3Ul00. 32.000: *as.ooo. 30,000. .32.000 3p.OOQ 3BJ:l00 *az:oou N. 10 *10 10 10 10 10 10 10 a .. s '28;000' '31,000 '31'000 *29,000n.0,00 3,4':..000 37:ooct 34--9(10 3q;Oot> 37.000,. 33:00()' ,36;00Cl.siooo* 1*Tailed' t-'Stat* ... Critic::aj .. -1.81G. .3;oes 22.s3S .5.449 2.281 ?;281' 2:-287* :<:281 'Critk:al 1,035 15.08&3' '10 29.000 30,000 *3ZODO .3().()Clti aacoo 36.J)OO* adgo* *rsoto.nic MSD Milan1.COOI}' 2;014 32.817 MOOD :zo14 3Z.S17 1.0000 2.0.14 32.81'1 1.0000.2.!)14 32:817 1,00@ SkM.Y"' ....* Kurt .. Lin*oa.t 11\terp.olaticon .(20!) Point *% *so ICOS 1c10 1c15 IC20 IC25 IC40 . IC50 "'100 >100. >100 ::>1QO >10i:>* >10.0 >iOO . 40 35 30 g25 " = -e 20 Q. 15 tO 5 o. c Page44 of90 "! :0 ,, " 0 .u ss% ct:*. *s1tow ...., . "'-* a:i 0 " N "' e .... .. 1.c ..--------------.0.9 o.a IW *D.Ei :: o:s' gM : o.z 0,1 op-e.-.D-C..,_...,,.___ _ *;:*.: . .,.,. .... * -0.2....................... .,.......- ....... p .... "" :g, *S TVA_ INudear Outfall 101, -Intake August. 02 .. 09, *2016 Statistical Analyses . Ceriodaphnia survival and Re:pro_duction Test-Reproduction Start:Date<812120'16 End Date: aie1201'6: Test IQ: --Sample m: iVA I SQN Outfall 101l:,kibJD:_ETS*E'nvir. Testing Sol. Safriple Type: D!\l'fR-Oischarge Monitoring Report bate; Acigust Protocol: fWCHR-EPA-821:..R"02-0.13 Test Species: CO-Ceriodaphrria dUbia . .Comments: . *Cone-% Corttro(:.t .control'-'2 . 10,7 ;21:4 42-.8 8'5..6 10()" .(ntake Co.nc-% Corttrol-1 contror-2 10.7 21.4 42;8 .aa6 10ll 1 '31.000 29:000 31;000-34.,000 :32.000 '37.oOO *35;000 2: 31.0QO a3:ooo32.-000 35.000 a2:doo 33.00ci -a_ 31,QOP :;1;oeo* 32;oop 32;00() 35.00Q-$4.0004. 34.000 :29.000 :35;000 34;000 31.aoo 36.000 31.0PO 32".000 5 s* 32;000 30.000 31;000. 28;!)00 .39,000_*as.ooo a2,uoo as.coo-33,000 34.QOO 34,(lCio 3$.ooo as.ooo 35,0QO 37.ooO. 38',()00 30;s_oo 1.ooa? * -so.600 -21rooo* -34:000 5;597 a0;soo: t;oooo so,500_- s2.2oo 1.os57* a2.200 35.ooo aoo1 s2.aoo 1';0$90 :3_2.30o 30.000 3s:ooo 5.s62 1,091a 33.3Q93Q,ooo 6.339 33..100 1-0852 -33100 30 .. 000 36,000 6.440 35.-4bo 1*:1607 as:4oo 31,,000 5,835 1.1311 32J3oo. JfLOOO s.995 .1 3'LOOO. s:rnoo 35.000 ao,ooo 3;2,00Q aiiooo 32.ooo N_ 10* 10 10 10 10 10 10 10 8 9 10 29.000 2.9.000 31.:000 31.000 30.0C)O .29.ooo 32;000 31,000 34.000 31.!lOO 32;0(}0 a7-.ooo 30.000 34.000 3o:ooo 33.000 3_7.000 33,000 36.ooo J6,ol)O 33.000 34,000 1;;Talled-t"8tat :Critfoal -MSO -4:94Q 1 ;734 -1'404 Auxiliary Tests; -** :StatiStic _.***------ _ Critieal _ !ndieate$hQtmal.distrib-UVqn (P > 0;01) F:.Test {p = ();36)' . Tne. control are not sisnlficanflY.aii'fereot'cp_-= 0:89). . 0;868 1:87805 0.13853 -<.10092 MSDu MSOp M$B H?moscedast1¢'.t M-$ignlficant differences 1.49401' 0:.04603 80 . treatmentS vs ContrbH2 --.2'.25_-u..... Q; 15. --10". 5 Dose-Response Plot g* ;* t: : e s ,g 5 J; o* o-Page45 of90 1-tail, o:os level of sigriifii::ance MSE 3'27778 .Skew 0.22443 F-Prob 1, Kurt -0.7205 df 1, 18 I I 0 I I I I ' ' l I r ;Specfes: Client: TV A I Sequoyah Plant. Outfall 101. Daill Chemis : * *Concentration CONTROL 10.7% 2L4.% 42.8% 85.6% IOO,% 100% *Alkalinityi Intake ( *CaCQYL)', -'r *Hardness"':L: (mo CaCO;/L) "'TR chl!)rine (mg/L). *Tem erature C°C}. Qate:.,os4u.:16 "'Temperatures p¢qtmed atthe time of test *i!litiatioo. ot temtinati()nby the Daily Renewat ' !nf(lnnation on Page 1. pJbliitity, hlirdnerui.arui:tqtal performed op;t!JeJieqph** sheet specific foreadunalysls 1111d thili bench sheet by: :'/A . age46 of90 \111* iP !;! 5 ::::....r_,.,,.._,.._;Page7 of7 Date: 08-02-16 Coneen.. 'Parameter tration CONTROL llA% 42.8% 85.6% 100% 100% Intake *ffar$Incis . <m. CaCO.;/LJTR cblorine tmgtL:J *Tem ernture {"C) *TumjJeratures pcrfonned nt tlietiine oftest initiation, renewal or termination by the analy.;tidcntified .in !he Daily Renewal tllble.**'ocatedon i;age .L and: total perfof!l'led by tlie*analyst identified on the bench .slJeet sPectfic :for each analysJS and tlHliis bench sheet by:. . , age47 of90 SOPATI l -Exhibit ATI l 2, revision I 1-0H4 Concentration Parnmctcr oU<Sm CoiidlictMtv tumhoi/cm) Tem111:ratttl'll'l"C\ .nu1sm. DO trnulf .\. 16.7%Cond11rtMlv litml1os/cml nll CSlll DOfn111/Ll . Coitducilvltv tumhosfcm\11u.csm DO line.IL\ , Comluctlvltv l11mhosfcim\ DOtinl!JLl*ss:cw .. CtiniludMlv,lumbo!lcml . 11UtSm Cotiductlvliv tumhoslcm\Alkilllrilty htil!lb CnCO>l Total ltcisidual.. Cllfol"llie lm!!IL) nUldJI Alkalinity hng/L CtlC0 1)-'roliil IteslCli1111* tml!ltl TVA I Sequoyah Nuclear Plant, Outfall 101, Non-treated August 02-09, 2016 D11vo Ceriodap/mia tlltbia Chronic Whole Effluent Toxicity Test EPA-82l-l{-Q2-0t3, Method 1002.0 Pally ChemiCnl Analyses Dn'.l'J. 011rl _______ 1_16_3_7 D11y4 t>nv*6 ** ,(l!ltlUI .* Fi1111l 111m111 Flmtl ** lnllinl FinallnltlBI lnltll\1.Finni ll1lllnl Flmll fulllal Fin11I . 8.09 7.92 7.88 .7;86 1;80 7:89 .7.83 . 8i03 . 7.8. 7.8 .7.8. 7.8 ?JI 7.11 7:8 8.0 308 *.*... * ; .. .-. ;. *Jlll :'*,. ' ..... *-as *.-.. ,., .*. _.,. ** * .. ** .. i :14:1 2s.o24.1.. ... *s.06 . 7.91 7.9S . 7,SS . 7i:l4 7.89 ,8;02 .. ..... 7)1 7.8 *1.9 7.9' 7.8 .Al.O :20s 24:8 .. .25;0 7.94 7,95 7.90 ;7;.89 8.01 *1.9 :* 7:8 7;9 7.9 297 "'* ...... *;. .s;o3 . 7.93 7.9S . 7.86 . 7.90 7.89 7.96 8.03 .. J.9 *7;8 7;9 7.9. ';7.9 8.0 263 ................... , 24.8 .8,QI * *. 7.97 7.91 . 7.89 7,96 . 8.04 -228 ;'.' *; * . ..:.:f':: .......*. 3,01 .. 7;97 . 7.98 .. 7.90 *1.92 wo. s;oo * *:s.os * .. 1:8 7.9 8.0. *7,9: 8;0 7J) * *8.0 ' SJ '.: .. ' . , .. ;-;;';'. 2$.0 24.7 !?*k9 24.8 24JI lS,2 . 2'-0 . " 25.0 ' 7,96 7.96 .8.00 7,92 . 1.93 us. 7.99 .8-08 8;0 7.9 . *'8,0 ....... 7;9 8.0 ... ' 7.9.' -8./J '* ' 8.2 7.99 i.92 7.73 1.97 7.94 7.99 7.8 8.0 7.8 7.8 318 ..*. 317 ..... . . "59 , ... , .. ';i : .. * .. *.:: .... 90 *:*.::.:**'."' . : .. ,; --**-** -.... , *..*.. 24.7 . 25;0 ..8;12 MO 7,91 7,97 8.10.. "91 7.9. 8;0 s:o .* 1.9 -1.9 .29S 306 . **309 *.******** .... 24.88.10 7.90 1.92 .. 8.0 ,. 7,9. 7.9 '.;283 : ... 296 301 8.09 . Ml 7.94 8.03 7.93 s;o 8.0 8:0 1;!1 1.9 s:o ito 7.9 s.o *s.o s.o 8;1 I " 7;93 .7.97 . 8.1l6 ?;96 2$;1) %4;!) Jl.IO 7M 7.98 8;2 8.l *tkl 199 ,, . : .;': 305 8.03 7.9 ... ,.:*::*:-:*,:**8.06 8.l ,211 '"'.,, 24.9 7.98 8.0 . , I . \ ' (i.i . i11XJ*Entered by: J, SumReviewed by:+.Arialj!St .f}fp CalihtaiUm';. .. Total Residual Chlodne (OJnON-1977) Matrix: Wat¢r, RL"'0,10 mglL ' Page 2( Page_}__ of_/_ Meter: Accumet Model AR25pfUion Meter . ,. Iodide reagent ,1---'/_N_JZ..,,..]=_.__S_"l> __ -t . Acid reagent: .....uo.mglt NQtq.*'Wlifr a chlorine bf ? LQ mg!(,, the samples miistbe;diluted lt> be withfotbe ealibmtion range.* Laburatorv controista11ilanl: number Sample number 'True value (TV) . ,(mg/L). 0.50 Sampie: cha'ractedsncs sam;ple Sample ID number, Measiir value' (J,\'IY} *(mg/L) . * .Residual chlorine * (m IL) s,to .. %RS='MY /TVx 100 (acceptable range=.9D to 111)%} %RPD = f(S -D) /[(S+D)/2 x 100 , .. *acceptable range =::: 10%) >>£ Sample Residual chlorine (m .L) LO-oo/i/r standard vnlue (TV) Measured vali.le (MV) " % RS::;:-MV /TV x 100 n.umbet . :(mg/l:,) (mglL) (aeeeptable rn.nge = 90 t1d H)%) Reviewed by / (ft Dote* reviewed ._t-_-__ Page49 of90 SOP C8'-Exhlbit CS. I, revision OI-01-13 I I I I I I I I I I I I I I I I I lf _'l_* T:C),tal 'Matti!<: Wa$7, R:L =*0,10 mglt Analyst ING * .Date ,Meter.M9del A.R.25 pHflon 'Meter 1*::::: 11:m Calibratitm:; *tr:ue.v:JJµ_e(;f\I} number {mg(LJ. Sample number. recisioi1:.. Sample IQ .* *saniple Soni le measurements: Sample S'ampfoID number 'Re5iduaH:hlotitui m ft. . . s.jjp.,£170'% ffSi=.M\t;ti'VxilM D *-* -----""_._..,..mD Reference standard True. (l'V) Me.asurid value* :(1\-'lYl'° %R'S'= i\:lY'tn.r,i number (IJ1g/L) * (ing/L) ... * '*r . I(,* . . . Date reviewed; ..Page50of90f0Rt(JN.:t977lMatrix:Rt = l:U o tng/L Page. 28' _of _L_ Meter. Accumet pH/Ion Meter * .. llile=:li--a-1E . J * *::: -.Note; of >ihe samp!$.11iust bci tt> be within tile calibratio1J i'angc, . . ' . . . . ' . S:u:nple* .**number (tv) * (izjglL) 'Meas11red'valu.e (l\IV)' . (ffi,g(L).Sample cha:racteristks N'i11e: . . La/JoiiitO... *c0ifli4J:startdat'lf:.. * *nutilblir:Page51 of90 (TV)* *. tn'ij:/i:;), . ' * ¥:du¢* '(MVl (lngtC.l 3 RS= MV I TV x*lOO * {!ltCcptab!e range= 90 to Reviewed b_}r 11-. _ __,M------1 Datereviewed ..... ... fk..._._,Jli:.=-* _ __, SOP C8-ExhibitC8.l 1 revision 01-01-13 I I I I I ._J J J J Piige; I *Of v Analyst' 'I v-*I.*. Dateanalyzed ..... .-'.00---..-w- ...... -'""I .* Titram i1omwlitv.and:mu/U11/iet-tfetermb1atin11:. pH of l'ltmnality1* * * Deionized T:itrant ' 'Eiid Total. =($'-miNaiC0 3 x pH=(Nli. $JOOQ)IIO() l1lisan1ple* ::N;c,.'.so.o . reference . .mt : ml ml c= . =4.SS.U. number number <El*.standard number Sample number *True (TV) {mgCacOJi'M . .J Duplicate (Bl Matrlt soike reco11e,.Y:Safllple yqJttme cin1. H)O . ::.Atki\liniw**@IY) .100 Begfu : End Tow.*; M1dtipner* .. (11.ccepta:blc.nmgc .ml ;ml nil '. . * * * ::"'!10 tQ:tt!);.OAif . ** *... -*,\\:ll(li6riity .. . $RPD"=Total . (iilgOiC.Q#Q. * ml . . <*'11>l;abie range =.;l; 10%} . * .** t{)C), . ;S Reference$ndard Spikla Sa111ple number .. '(SV) volume "(mi!:CaCO.JL) Jml} , Beg!n* *ErW: . Totili ' 'ml ;nii. i Samole mtas11rf!l11e11/s: Sample number Page52of90 alkaf!nifY (B} . (mi. \jat"uei\'I:V,,;;;A.... :i,r . . (me. CllCQVL} . Begin ' l'crtaJ %':a=:MV /SV,x:lOQ - range =-1Sto:il5"4'). fin.I} inf . , m1* . ml * * .Muiti1>1ier AUcanilltY (me CnC0.1/L) . " ru* .., U* .* ... ,(po **.* ..... $:ia 1 l *1 --1 1 -*1 t I j . i l l J l J J J _.J o.: :0.: .S..LG, ** .. f A.lkalinity. (SM' 2320 }J;..J.997)Mattix: W.atet. Rt= C'9 mgCaCQiLTitrate*saJ11ples to pll = . Page Pa.,,ae . 2 of_V..___Time initiated ,........_uQO..llllts Time completed . """""" . TitraniutJtm.atiiy Normilify heck :I. Total ,..fererice . : *.. mf . :Normality,'(N)"::i{S ml'Na 1 CO;J x\U.1)5)/E'pH Factor ot Multiplier 100 ml sample * --iV.x.500= 4;ss,u. ri *number_ l Mmber: 1 1. .* 1 -mi* *abic ri*=i'o:Q.1so .. ... ., .. ......... Ui.b.t1ril,tofY°CQi1tt..Ol sltfi;tlti.td:.', **. . *.(gig , (m.l) "**i *Alkaliaity (MV) I J**j Mtdtiptter**ml . mt , ml ] .. 10tl . t 100 .. 8 I lO:<f *1 qs . $ampte:in.. .11 ... :... ;,,.ove"'** ..* ..._ .. , .* *..r** Sample -volume J. *.1 :End 1 *Total *(ml) .... ml * *ml . mr . .IPO I *}t'o;o 1111:1o, .J; J J.21 .. l Uo*S:: MiiH,ipliet I (mg CaC03lL) J 1.s Uu "* fD 100 %RS=MV /TVx 100 (:u:ccptable range =90to 110%) qe.01, %RPD= {{S -D) /[(S+D)/21} x J 00 (aeccptablc range=::.10%).,. ' ntlmber * (SV) Sl:tmJ>le*(l'll1) .'j End. I Tot.al j\IJilltiplier Spike alkalitiity (A) * {mg CaCOJil..)<rim' cacil31.L> , -ml. ... ml , . ml*50 \t:Q l\\"" I ISSJ ;fo:.o lSb *samtJle*lkalu:iitr(B) . ** (mg* * .. . l !)°() . Snmoie -Sample number-P.> :Sainole ID ... L*ebJ .. . . . ... *.. . . . . J . .,*Jo: J. lhb: 0()\ . l . !fm:tl\)T1* I . ,It. ; _ . rrll 60\_ * . . t)g:Qb ..J. i'i . :j <ft . . .lb ...... :-\I. . rm1N" ., .. *1** I' . . .L . l'-rW6A l * .Sample .votµ-me * (mi)' ..* '*:.oo > ** , J -26' .ffij. .... 100 %'R=*MVISV:x: lOrJ. . (lll:!cepilibf¢: range =7Stol25%) Begiti .* , En.d I. Total -ml . *mL .. ml.* Multiplier ,;t,.tt 1.$l1 I G.;1 1 415:01 'I 1-J]'l..1 I "Li I ..:a.a 1r1"\ *t .... -.f, . -* ,,-., .. . . ----.... JJ,ti. *J.1s .. 1 . ........... *> .J-""**1: J ."** .... 0-** .1;t.t)*,,, ... , . LJ.J4 .. . :"I .W io.ol:tasJ a.s . '25.tP. l:q, I' 25 7'-1*1* l l f . . ."::> . '"--..(",, . ' '°1' 10;{) ' 1£ 100.w. ........ .Alkalinity (ing CaCOJIL) qq ""'**It280 *"(JJ ro q-z -=ro 313 '-IJ Page 53 of90 RevieWed by: I .'. f Date reviewed:I o&t>\.*1 b C6 -Exhibit C6.l, revision 01-01-I 3 j J .J J ,Afkalbiity ltJ Pag&. a or. 'V Matrix:lU, l,-0 D18 Cf:!C03'1;;.Ttttat* pH= 4S s.:u. Ti1rm1t llormtilitv a11d n111lti1Jlierileier1niliatiort:' . I.:. pH of *. Normality Deionized Titrant check' ' Begin End: !' *ml ' ml*,f9tii#t1it;y (JVtof'li'iSQ 4 =ts mUii,!hCOs . .. _(JjS/jliP.¥ JfActot. or :M:!lltiplier= (N'X :.soooo)l 1on*m1 .. '""'JVx,50(} water re1e ...rd . 4.5 . number . namber*' Lnboratorv eo11trol stamlard: . Reference stru1dani' number 1'.ruevaiui (TV) (mg iOO sa,rople.*voiume (ml) JOU ; ;. ' *able 0:1!220}: . Begin . End , T9tAI, n11 1111* 1111 . i A!kalin)iy,(M\l.l, ' ,;<JOO , (mg-'CaCO$Ij)* * = 'Sataple .Alkalil!it;y%RPD=* ; v9lt11n¢ .*., Total (mg<f\.C.OJ.14 . *'.(ml). .... tiil: r 93ef ..... iA.o* ... * o, Duplieate (B)' Mauixsoikerecov.erw Reference standard : Spike val11.e number (S\I) ***volumeBegi!'I , EndTotal J\lful(ipller (me CaC03/L) lini) . iiJI . mf . '. , ml Smnple 11ieasure111e11ts:Sampi,e . ! . *****MV=*A,,,B . {ingCaCO,ilb) . %iR=::.MYfSVx100. ,.*. I , .... 'O\' . Samole number SamolelD 8aJJ1ple vp1t1me Begin. * En1:l fmti ... .J ml' 11t1,ml Mulfinlier Ait<aUnity*lmg CaCi>.J/L> .\f.l," , Page 54 of90 *'j: l l J J I { ' Page .Page '-l of lo .Alkalinity (SM.232Q M!itrix: Water, RL=, ' * . _Tbne initiated,.:'>... UPo\\I Titrate sam pies to Time completed "'ill' ...__ _____ __, Titrantrzarlitaliivllf1d i11t1Jti11ifer ife1em1iltiitfon: . pH of: N'ormality Defonizecl Titrant 'Clteck; '\ &Le, : -* *nee stitndard , , . nu'niber *: numoer Lb It: $fan 'ttii :.** Truevabie. number . .. *. '(mg.CaCO,Vl,) . \ J;90 Matri-c sJJike t¢e,overy: ' ' * . . number . {S\r) * (iugCaC03'Ul (N) of<l:liS0.1 pH Facror or Multiplier 'IJeginEnd * ,'to,t.11=:(S:mlNa:iC0 3 x Q.OS)IE ml, m* m'i *tl:.25/E * = (Nx 50000)/ )()0 ml sample Nx500 m'i .. , tai:teptaJ:iie range>= 0.0220)Alkalinity (MV) % RS=MV /TV x 100 volume 'Begin '*End Total (mg C'lCOJIL) (acceptable range <<mn .ml ml ' ml =90 to llll%) lOQ .leA 'Z.e.e ct.ii:\ toLo qq,b/ S.rimpleAlkalinity%RPD= volume Begin ml . *End Tntat * ' Multiplier {mg CaCO.YL) {(S D) /{(S+D)/2)} x IOI} (acccptnblc range = +/- 10%) ml ml vriiume Begin End Total M*uitiplier(nil) nil mJ .. ml JJ:sZ Spike alkalinicy (A) (mg CaCO.,/L)CBJOitoa!LJ 1\1eas11red spike vahie*(MV) lWV'=A-8 . (.m1t CaCOJfLl % R=MV1SV:x100 (acceptable range =75 toi25%) ' Sam pre number : Samnle lD Sample volume I . (tri}) )OO I Page55of90 m1. ... End nd' Y*lil-2.1.sS:* tD0.0). Total ml. Multinlier.... I o:o Tl I Alk:tlinity (m!! CaCOJiL) Bo SOP C6 -Exhibit C6. l, revision 01-0 l-13?.'\' Analyst I :a . I t Date anatyted . ' i\Q. \p. _l'a$e ... 5 of l a Titra11t 11ormalitV a11d 11mltwlie1'<det.enniiu1tioit:, pH of Nonnapty Deionized, Titrant water reference 4-5 S.U. number number Laboratory eonti'ol stmufani:Referenc¢ standftl:'tJ number : Truevalue' (TV) , volume:* (mg Ca(;Q.VL}lml) Matrix snike lf "* * .... e.gJn . ml= (S ml .. . , =@.l5tt. . pH Fl(tfoi' pr Mll)Uplier . * . .. -soo*, . . . .. End l Tohil ml' ml Aikaiiitify (MVJ % l'V :X 100 (ll'!g.¢ilt<WLl* Spilfo;value.number * {SV) 'Sample= volume . iif;!gin . End .. *. *(Din Oil .. ml total, .Multlp)ier !ml* (Al t':lg()JJL)* . Sam le meos11remei1ts:Page 56 of90 *. Oi003'L) , . .......,,,,:., -f'Q '*= . (me caoouty .. ADY' .. .. *t6Q: . "';' 75:19125%) .. Reviewed by* r . f t .bi* .1 y ** * . ' * *&c* ' SOl?;Cp.'-.Q.hOl l l l *1 l 'l I .J _J J l J J J .. Alkalinity ($1\1] 23ZO B.;1997) 'MatriX:llL = l,-O mg CaCO;/L to*Page "'3£g Page (.p of Lo . .Time<initiated t :.............. <Je01lt Time completedNotjtitditjr*-* .* . ,* *:Begin ; : Totaf * . ... 'U ;..1-*, bclbn.tea***Titntrn; pH Factor or Multiplier Ntn'malify (IV) ufH 1 SO-t ' ,y#.ter * .-"::<4;;5,S;UL.<1ttimbet. .$fandiirf$. r ID* * ... :::iL . ml number * * * {Ef : ;;:_ (5: nitNii:CQ.l x itQS)fE..= (Nx.50000)/.100 ml sam1>le =Nx500 sta11ifard l'tu1' value Sample . * . Alkalinity (MV} volume Begin Ji:nd *Ti!tal ** Multiplier;: (ing CaCOvL) %RS=MV /TVx 100 '(acceptable range =90to 110%) numb.er , ,rtnl}, ml ml ' mr ' "Page57of90 Silmple AlkalinityBegin-*.'£n:d*Total Multiplier (mgc.1co,/L)%RPD= -Si,tb!plcffD. -volume : <mn . ' rnl * ' ml ml ' {(S -D) /[(S+D)/2]} x I 00 (acceptable range= ::1: 10%) :Spike value ' (SV) * . voiume :imreOiCO.,/tt '(mil $ample a1k1!Jiiiity:(B) cBCOJJL}. -Tctnl : Mt!:ltiplier '. 'ml. * . *ml * *mlspike . MV=A-B f. CaCOalL . ;*' : Spike alkalinity (A) . (mg CnCO.i/L)% R= l\iIVISV'x IOO (acccj>tablc range = 75 U5%J .OJ. Samprevolu1r1e Begin* End* TDt81 tmnml . . ml mi Mtdtiulier Alkalin'ty CnCO:i/L}no 3000 ZCXJO ?.1-v"JQ "2..tbO -"'""'"* "* . Date reviewed: I t>&*b\ii"l!o , SOP C6-Exhibit C6.l. revision O 1-01-13 .. 1 l l l l --i I l I J J J J .,. / 0 '!l r i" ., i * -1 ... r--; s :_____i --' _./ Analyst I '. I Date analyzedf -----(SM 2346C.-U97)RI,,==* J,O.mg . Page ' . Gf _j '------ initiated:1*.ti..fe I Time complete<t....... :_.ty..:;*__ :z. ...... __ __,_ Tiirant 11<Jm1alftj1 imd nmlifpiieiileremJii1tlti01i:.TUra.nt.J;onn***lllity cl .. 1eck1* ' ile¢in. 11 *1 T-0ta. * .t refertWte . ml : ml ml number . .. HI number .. . * . (E). 'Norm::ility{/Y} 9f EQTA . pl:t'factor or Multipiier = $.t)OQ0)/50pd sampie =Nx.1000 t NS4*,"Qafl 6.o '! no t to.o *0.02.00 . Laboratorv -con/ro/ sta11dard:.Reference.standard* number1: Sitmp11?. (iiV)* . : voilime m caco 1;; : .. (. g ..... 9. ...... ,,{mil 40 1 sc;. *recisitJn: .ml lO.;.u BegiJ) rill End *1 Total . 'ml* .. ml .1* WRS=.MVI'r\i xlOO 1* {mgCaC03'L).. . . . J t<!l . I 20.0 End ;I* Total* I Mliltjplier .1111 : mt "" Hatdness (!JigCaO>Jll,). fo5.01 %RP.D= {(S.;; D) /l(StJ>)alh 100 "l t '*' .k1 I zoo** s 110 Dupllcate {B) l. Matrix soi/re recoverv: Reference .1 va* .. * .. *. '. *1number ; * .' ,.. * .4o ,: 1 *5b Sample 111easureme111$:.$ampJ,ehardne5s (B) QO l"tl )fl-:1 ,1 a':'o J. . e>. *Leto*. 1: Endj Total [ MultipI,iet:: . ml:ml ml hard11ess{A)'(iitg, (:!l'(::()jfL) ha.t *I Ll a I tcJ.o.* SJlike."'1lli.te(MVl MV=A-B . . {mg QC()j/L). '-lo l?>D %iR=MV1SVx:JOO (accep:tahte range . . .. Sa.mole number .* $amJ>fe:.iD Sarrip::Jf f r . Hardness ... CaCOJffil TV=:ND '.Blank {slioUld.be=O'mgCa©il:). oe.oq,Jk I .ssw lo6ot.lv fl I mHG:.W* g I 1 I 1 sst;J f -m Hb.trJ* 1 . @* 'Z(o.4 V I f --cf Q'}.t9_.U4' 'Sb . o.o I o'!o I .20.c;>. .l\C Je.1 1 *io,t I t.oI Lfo.I t.1,;.e. 9t11: *a1;q 1'1.i-f I . tlo -jl;c.t l Jl01 .Lf .* 4 B*t5** *.4* 6 I. z:.o*. , ;.;7..J *... ... 40 Cl2 )'.IJJ.wqo Jltfl8 4 <llsl&::J.0 . ., 92. O*D IAet'IM Nore: If >tSml ofti.lrant is be-diittteq.Reviewed I '& I Page 58 OT 90 . . . . . Date revieWed I. . I so P C7 - C:7 *. l l l l -, l *1 J J _J .l J I J J J J *e g ****b J. 1. *06.D5 .. _ .Hardness (SM Z346*C>l997}1lL = 1.JJrng caCOIL Page __ Time initiated , ........._ , <8$f " Time cOmpleted . .........; t1'1ra11t iiotmolJJv* :., .* : : * .. ""' * * .,. ._ ....... k .. J:iegin * stanefatd*m1 *. * . tu.imber*. *number Labomto!Y:contr.oistanlliirii: True. value (TV) . '(*volume {mi}' so : .$a.mpk:vofume 'rid) Tofii.J. ml . (:E): .. *Notmalitj.(.tV) ti( EDT A ...o.2'!E '. * *'-le o;onol pH 'Factor or Multiplier . (Nx 50000}/ 50 ml sample -.Nx moo Hardness (MV} % RS= MV /TV x 1-00 (aecepta bte .r:ingc Beghi End' Toint ml ** ml ml (jngCaCOj/L}= 90 to 1 Hlo/o) .Hardness %RPD= Bi:gfu .. End Total Multiplier (mg*CaCOJIL) {(S -D) /((S+D)/21} x 100 mt .. tnl ml: ' 61) . 6cl 2.z i *io.o s44 .. ..J. , Diiplicate (B) *1 4 . ; S.t1 ll>i0 Referencti starida:i':d $pike vnlue * .$ample 'number . . * (SV) . "volume Begin : £nil . . (me'caco.Jt)(ml} mtnil 2;z.. Total inf -1 (B) . 'Measured spike value. (MV) :: * MV=A:: .... B* (ing CaCOJ!Lf 40 0 * .*Begin *Eud . Total 'f4 ---..oeost.ip Spike hardness (A) (mg CaCOJiL) %R=MV/SVx 1.00 range = 75 to 125%) IQO.lfJ. Samole-numbei* .Samnte m .. *(mij. Jril mt' ml Multiplier Hardness (m2 CaCOJfLlB .. l .. ..:'" .... T\f===*Nn* * {sn0!.'11<1,be=u mift;aUJ:ii.W ?,Un llti -L 2.o se Ll:z. -&JO . SOPC7-ExhibitC7.I, revision 01*01*13 l 1 l l l I J I _J J J J J 0-I ' I '"'""*** I \ L___i -:--.____ _, -* Analyst I IN. Date analyzed . CJ6.,Qs.iy RL .;;,. )..0 mg; . Page Page: .a: 9f Titrant 11or111alitV and nr11/ti11/ier,lfetermi1iaiion:Titrant Normality check Begin * End *' Notma,.fy(Nj; iifEQTA .* .(;a Fi,ict9r or.l\'iultipltet* re!.A_ .... __ ... * "'number number fE). =.*** * ,:= .(N:x SOPOO)I s;unple ...... _:_.. =NxlOOO Laboratory:co11trotstoi1dartl: , Reference st.'lildtJrd number Sample number True value (TV) *, (mg QJiCQ;i/L}'. J) Matri."I: spike.recover_v: Reference .standard .Spike value number * (SV) * (ml! :CllC03'L) .*Sample. voJume (ml)' Begin ml* ilardnc$s:(MV) .. 1011 :&nd Total* 1"ftiltipJ1et* (0tg* {ttccci>*alile tnnie so SnmpJe vofoine .. B¢gin End T$1. . Mu,ltipljer (ml) * . nil ml . *inl * , , Li"'. , , """"'.*.',...,, .*** .. ;.;.J .., **'4$.1:. I* l .. *. ' Sample **volume . (ml) . .50** End101al ml .ml', ml* Har.dn'55 . %RP.n.:. {mg:4,CO,iL}x IOD s .* z.z * ** , .. $pak.& cA.l <:acoJII.)Sl\mple'lmrdness{IJ){mg*CaCQ.VL) Measured.spike . MV=A-'B . % R"=;MV tSV':x 1'111 .. .. =..i7Sto*125%) .... Sample meta11remm1ls: ... Sample number .Samote:tn 1 0 I ... .{iJ!aOit:OJILl $itmpfo *. y,oJuine * 'Bigin. :;f,Otn) '!miOIC:Ll tmn int ml ml . . Mwtinliet', ' , ' , *. 1 .... \'J , ' : . : : [ J ****.SOP C7*;,,,J, revision P 1*0.l"'U l l *1 l l I -i ., j I J J I .J J J J 0 IE:: :;;t; Anfilysr ,-l;C>.----**-r J)ate:anaiyzeq, . OOX;Jt,.*lV. . . . . Hardness (SM l340 RL ,.,,. LO mg*CaC03/L Page Y I I . Page . of b .. Tiine.foitiated ---] Time completed l-Sa..1 -. Tltmnt ;1pr/11'1lftJ(tl11<l n111itifiliet,f.teernlii1at/Qn:. Titrant *1: No.m\iility:tftel:.k':j* 1* End *I* Total reference*.*standard..mr * mt* minibtr .. *. . ilfil.llber ml tEl NcirniaJity (NJ of'EDTA . (acccptilbJc rnnge""... O.IJll(I) pH Factor or Multiplier = {N); 50000)/ 50 ml sample ;::Nx 1000 tr¢5sl3a7:l o.t) __ ltt*<1* J *q.e a.01.otl ta4 WtiriJJif!Y °(:ontPJI $/mtdai'.11: .1 ** trtu ... .1* ... G;iCOJIL);.(ml) r"N.ss1x:i02, . '. ,, ..... . 4Q I so . .sample;* . '\ .. . volum.¢ ... *-. cm'o ' '51J j 1* End J*mt .. *. mt . . nil t t'l *Begin *1* Encl , . Total ml. mt . ml I ... I t'l-. .:ifamriess (MV) Mattiplier I OiCWL) .aq .Hardness MD'ltiplier I (mgCaC03'L)1;aq *1s* 3r . Io . ,;;-:.! .. ; . .%'RS= MV /TV.x 100 (aci:eptable.range '=90 to BO%) q'l.-5/. %RPD= {(S-D}/[(s+D)/21} x 100f * :Sp!kevalue.. , .Sampleoumber '.. ' ' {SY)*._. ' . *. **1: Total. I Mi!ltlplier:.*(mJrCaC-0.JL>.*. ___ (ml)-. .011 :ml . ,ml. Spike hardness (A) . (mg CliCO.llL} . . .qo ,L <<a.J 1 .. "3.1+1 .... 'tQ.q ". 0 Sample.har4nts!i-(B) . . Measure<l spike value (MV> . ** 1 . l\'IV=A*B 100 {ac:c:cptiibh: r:tugc =75.to125%) .a;; 8an111le. i1!.eaS1tte111e.ms:: Samtife number l . Samt:ilelD TV=ND Bfank . (shiili!d'bli-=(hng Ca*03'L)L Jfi. J 1 3 . :u., 1,f\f Catu. t . ** . t . z.: l 3. -11,; I :.s :6:<<-u l Ir. t j.. l\$b.&.O\i..n .. ii; J !J' ... . Sample* volume . 'lnll}. *t:."h.. OU ... 10 (!rig 4il '02.s*1. *Begin 1* End I Totalmi . ' mt . mt I . .Muttiolier o.o JO.O: f:{)-0 I to.c-{.1* ,. -'I ;tJ . ... ,, u.a. . .. . . J J. . 1.aa!1. ,I 3. y I l".f.bA *2.1 ... 3o J"32,5. I ... :.io l'.38.;tl. t .. . . Hardness (mg C:iCOJIL) µo laG) .. t.oo I.II 61 <DI 63 310 Note! byt f 'g\. I -Date reviewed J I Page 61 of 90 *_ * ** 0 SOP C7 -Exhibit C7.l, revision (} 1-01-13 l l ') i J l j J J J($M 2340RL = Lei mg*caco.;L 42.. 2. of .3 Tlttll11J 11ormall(V 1111d nmltli1lier determiutrlion: Titrant .Normality cbeck *' End * . Total referen-. mi ml * .nil. number number-* Laboratory co11tro/ stai1dard:Reference standard number Truev;tfµe (TV) {mg (;QCOJ/l,;J 40 2. Matrix spike recoyeTJ': Reference standard number (SY) (mg,CnCO..i/L) volume 'ml) voiume {ml) .H>' ..J voiume (mt} ',,;.., Begin End. *ml ml Be .. n End ,fitf ml' '!3..J :q.O,\ O*O '2;.°I *Begin End ... ml .. ml " ' Jb, *.o.Q q.q'No:rmaifu' (Nf of El>'.fA' ,' O.OllO) *pH* or Multlp'lic!r-.::*(NJ>SOOPO}/ SO :mlsample.Ns. lODG ,..'4:....., . . . -% TV x 100 Total * ... J.ier . (mg,OtcoJ/4* ml ,:;:?o.tUtO%).Ti)taf mi , .. .Hardness.* %Rrn=: ; :l\'.l,ltiplieJ:.. {m.g Cad>Jj.L) ({$,, J))/t(SfJ))flJ}d.0.0 . .. . 'S,Pif(e: Ifardne5s,tA> -**.500' sample 11arp)ies5;.{Jl} (mg-Measured . (acc.c:pfabfe ='7$,to US%) Sample melisaren1e11ts: Sample. number MV='A-B lme.C:\CoJLl, $,id 'total ,.. *aardn!!s&S.imiJJe.JD fmJ): . '.nil '. mt .. '.111i MultiDner (ma::Ca:CO.JIL) t'l. *-.' ' "10' *ex,.' I *soP C1 Exhibit Ci A. reviSion o l*Ol-l3 . . ... **;,. . l l l l l *1 j J ! J j J I J Hardness (SM:a340 C,1997) . til)el\I . , RL = LO mg CaOOi/L . . . /I.itt:imi uol'm!ffiiiltzndi11ultitiJ let t1,eieni1i1101ion; Page '-13 Page S of 2 _ Time initiated t8c)oJ'f "time completed """ .. i * . normant_Y.check 8¢gtnT'otiu ** * * * ** * ' m( nil N9nna*it.r (JV) of EDTA . '""*O:if.E' pH Factor or Multiplier -(N x 50000)/ 50 ml sample number\_. . -'nutnber .ii!>* * .. -Nx 1000 _ uE'.lw;11.J . . .Truin*al' Sample* . . nmnbet* Q:vj* , . . voifii'ne , Encl total .. i* * -CacbJIL.1* _{-*m* ll _ '** -ml .. ml ' ..' :/ . . .... l\fultiplier Hardness (MV) * (mg C11CQJIL) %RS=MV /TV x 100 (11ccept:rblc range . =90 to110%) Sample *tnuii:ber ' . :S:tmpte *_. * ' Begin End Total ("ml ml .* . nil ... ml Hardness(mg CaCO.slL)%RPD= {(S -D) /({S+D)/2 f} 100 . . M.atrA;siilke tlicovery: .. Ref'ert!Jlee ' Spike.vnltie '$tritple 'number : * 'VDltnite , Begin 'Erut *{ml) * :. ml. ml .. ., 'I;otlilMultiplier . nil Satnple.Mrdne$s cB>si>ike value-(MV) ' *(ufg:Ci!CO!!lb} MV=A-B ' (mgCaCOJ(L} -ero Spike hardness (A) .(l!lg CaC03'L) %R=MV/SVx 100 (acceptable rnogi? = 15 to 1:?5%J 10561* Sample vc"lu'ifie (ml) Begin End * .Hardness (mg CnCOJIL) ml rnr ml Multinlier ., . .aos -:716 . _.. ....... ------.. Note: Ji Reviewed'bv: I *I Page 63 bf 90 * * *** ** .1 ...__ Date .... I __ o.:.t....;;*{Jr_\"1 __ ..J 'SOP C7 -E.'chibit07. l,. revision 0 I -0 l -13 Page 64 of90 ' Sequoyah Nuclear Plant Biomonitoring August 02-09, 2016 AppendixD Reference Toxicant Test and Control Chart. ..-... -u _, .........tlO -en N > m "ti ( " Pimephales promelas Chronic Refe,rent:e Toxicant Control Chart Organism Source: In-house E!'lvitonment"t'testing Solutions, Inc; :LO '0.9 *0.8 CL7 0.6 0.5 1.4 .. 1.0 0.8 OA 1.2 LO . ..... ... USEPAtontrol limits(+/- 2 Standard Deviations} ...... ' ...... ' ..... , .. ...................................... ,, ................ . 'U$E:PAW:arning:*a11d Control tirnits(75th and 90thPercenfile CVs) ** . *** . . . *********************4*******'**,,.**a**.. .. .. ....;..;._.,,,.._.;..., .. ... ... ,-.. ., ................ .. .. ******** Laboratory and Control Limits {10th and 25th Percentile CVs) .... *-** **** :Ii.*** *** ***.-* ...... 11-:* .... -.:**. * ....................... *** ., ...... .. ... -...._..,.... ..--'*.*.-..;..............* .........................................1!'*0** .. *** ' Test date *7-day rc 25 = 25% inhlbition concetttratil)n.Ali estimation of the: concehtration of potassium chloride that would 2S%redoctiofrln Pfmephafesgrowt:hfortne test pqpulation. --tentralTenderit::Y (mean IC15) . warning LitnitS (mean 1c 25 +/-.sA.ld or sA.75) Contrc(! limits-( mean rc 25 :!: ;sJ\.25 , SA.90 , or 2 Standard Deviations) l Q) Q) 0 "'ti <c <::> Pimephales promelas ' -P* Envlroilm11ntal Testing Solutions, Inc. Chronic Reference Toxicant Control Chart laboratory laboratory use PA US EPA . Test number Test date 7*day1Cu CT s control Limns s..,. Warning Umlts s .. ,. c:onttol limits s.,,Warning Umlts s.,,. control U111l1S C.V 11!/L (a/lKCl) 0**25 CT 0 Si.:10 c:T+S.1,;o*cr-s .. ,. .. cr+*s .. ,. .CT+So.11 cr .* sA: .. a*s *. ,. l OH!Ho 0.74 '2, ;j)3*0N6 3 4 03*04*16* s *Ol*OS*i6 '6 04.()4-iii 7 B\ os,irfo; 0.67 0;10 0.58 D.(!6* 0.81 0;10* *0.61 Cl.68 om M9 o.7ll 0,10 *0.7S *0,71 o.os D.60 0.Sl 0.08 0.79 o.1s 9,51; o.ss a.21 D.411 0.97 0.31 0.$9 l.Q2 0,07 o;oa* o,so 0.82 o:os o.ss 0.14 0.14 ci:s2 o.ao MS 0.41 o.9.1 .0.30 o.ati 0.96 0;12 £1.JO :o,so 0.90 o:os* o:62 .citlS 0.15 MS o.9i; o;n 0.38 1.0t: 0,14 o.o.!i 0)19 0.87 o,oa *o.eo D.14 9.S4 0.1!2 .0.26 *o;4i 0.94 O.llT *o.99 0.14 0:09 a.s2 a.Iii; o:oe o:si rm 0,14 o.s4 0,26 D.43 o.9s o.u 0:38 l.00 o.ii o,oe Jl',.53 l).87 b.os O.GZ 0.79 0.1S 0,55 o.ss 9*2? ilAf 0,97 o.31 0,3.9 1.02 i.J.12 o.os o.ss 0,Bf 0.08 o>r& o .. 1s 056 0.86 b.'il* 0:44 o.!ia* \l.32 0.39 1:oa 0.11 9 OS*lMli io .0.94 . o.10 1).?q .. ci.70 0.08 0,54 o:aG 0.08 0;62. 0;79 o.1s o.ss (j,85 o,4s* o.97 0.32 l.02 O;li 0.01 o.ss O.liS o.o.s* .0.62 1).18* .o.lS .o.ss il,a!i o.2'i 0:43 Q.97 o.32 o,!9 Q.li u . tlli'l4'16 fr j3. ll.14 *lJ;7o *0;fl1)* o.n 0:63 *lfo 0.0? o.ss o.es o.os 0*.62 Q.?!l O,iS o;!fo a.as 0;27 0,4,4 .0.97 0,32, *o,ag* .l.02 0.10 0'.07 o.si O.ll6 9.09 0o1;3* a.so o.56 o:se 0,21 0.44 .i).98 o.ai 0;39' J.03 0,19 o.ci:i o:s6 *0.85 o.oa o.6t o.is. .o.so. 0.85 1u1. o.411 \).97 *o.32 o:a9* 1;02 0.10 .'Ol!*OZ*16 0.79 0.11 b.ti7 o.s6.* 0,86\1,80 O.lS o.ee .Q.27 0.4'l Ms ll.10 :is l(\ 17 18 ?9 to 'Noie: .1-d iCu " 'r*day lS"' fnhib1t10n cc.n,enrratfcn./\n cit. th! concentratlcn of pota5shin1chlartd&. th;t camft a 25% Jn Pll!'l!P!litiel ilicwtb fo.r the teJt pojiular;o,.,, q;, {mean IC)j). . 5;, ai t.lie )latues. C:on.1tol llnd Warlitng . . , * * . warning !inl.i.ts wer!!

lhll 111 the ic,,,v<).lves amd5tii Q'$,, The5.G tan&

int. * * s.,tii:"' Standard ec1ra$ponr11ns io cit. Standiitd tii tlJe 2$"' tv, (s,Jj ".o.2ij i:o1m:olamLwaroln&tim11s 5.,,. cilrrespoiidiri[Ha {S*,n" cv. ... D.4Si 'Cy"? var'llition or lhe ifi> us Enoiranm*nl*f Olt *;.F -at* 1.00 ;;r; n1 .... t! .;!! 0.75 I!) ii *- *= . c ........ 8 E -0.2.s. -r .;C: 0 ... z !ti 0 20 *;: *-m I!) > 0 .... 0 t... ... .... c s:: 0 cu u *o I;.. fE .E cu 8 Cl U) Q,. 10 Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source:. In-house ** ....... ,; . ., ****.***.** *.*.*** , ..................... .<'* ***********.**. .. ............ ,. . .... **'****:* . . . . " .................. '**** .. .. .. -* .. -*.,,-..;_, .. _. ____ ,,._. __ ..... ... -.-.,.--*-**-**-**--*** USEPAAcceptance (> 0.25 mg per surviving,fai'vae} Kentucky Acceptance Lh:nit ( <30.0%) ..... "*"."' . ** ........ ............. -*********** ............ ol!** *.* . .... t:onttol Coeffident of Vari;ruon (CV), or Percent Minimum Significant Difference (PMSDJ* ?Msb Is the *rninim um signifkaiit the control anci treatment that can be deda17ed; statistically stgriificant Central {mean ControfGrowth, OJ, or PMSD) -COntrol:timits' tm.eari Control' Growth, CV, or PMSD +/- 2 Standard Deviations} e Precision of,Endpojnt Measur4aments ) .... **-**** '*'* Plmepha/es prome/a$ Jleferenc:e Tt.Jxitan1 D;;lta Test number Test date Control Sun/iva! Control Mean '1 Cf* 1 2 3 4 s 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Note: {%) 100 03*02-16 100 03*03-16 100 03-04-:16 100 03-05-:1;6 97.5 04-04-16 100 04-05"16 100 05-10-16 100 05-17*16 1()0. 06.-07-16 100 06-14-15 100 07-12-16 100' Q],.,19-16 ' 100* Q8-02-l6 100 : Q.f.f71 0.5,1 0Ji30 o.451 0.647 0.541 0.,5()7 0.421 0;<155 0.5.78 '0.774 0.570 for Control-Growth' lmJ:IJarva:ei o.66i 0.664 .0;646 -0.611* O.!'iOS 0$7 o.s19 Q;5$ '0,!?84 o,ssl CV= Coefficient of, variation fot conrrol'growth . lower CV ,boond.!jetermined byJJSEPA.{10"' percentile)"' 35%. Upper CV bound by, USEPA = m% MSO = Minimum Difference PMSO = P$'cent Miormum.Slgnificanti;>iffefl!nce Cv 0: <91-J, for*Qlnirol ,Gi:oWlhC\11%1; 7E l.'3. 4;6 5.a s:Q 3,1 4.S M :5.z 5;!1 5.3 12.3 '6;3 1:0 6.4 L$ 5.9 5..0 5.8 . 5'6: s.s 5.9 .lQ.l 6,:a 3A* 15-,0 **MSI> ct (%) forPMSD(%} 0.07 11"8 0!07 8.9* -10;4. o; llA !),()$ li.., o:os. 8,1 11.2. O.Q4. M 10.7' 0.11 17._6 11.7 O,QG 11.7' M4 11.3 Q.06 '11.6 Ci'.()3 :7,3 . ll.2 .0.08 ll.4 o;U :i-a.-s. 11,5 OJJS 11.3 PMSO Is a meawre.ofiesfJirei:ISion. ThePMSO is the minimum statisth:aUy slgnificaotio awiu>ie-effiuenttollicii:y test. . * . * * . ,, . .. by 0sEPAJ1(1"' "'12%. UpperPMSO hound,d$termioed by:USEPA {9(l 1 h petcefitile) = 30,;,; CT= CentralTendam:y !meai!.ControJGrowih; CV,l>rPMsti.) USEPA. 2000. Undetstandiog ancl Accounting for Mi!t!locl Var\al:llhty In Eff.lue!'lt ioxlcitY Pn:>gram' US Enlilronll)ental Ptotectio.o.!\gel)cy, tincinna,ti, 01:1. . ' ... . , . USEPA-2001a, 2001b. Final Report; lntei:!aboratory' Vartabllity Study of E:PA Joxl¢1tv Tajty\ethOds-; \lo1i11rtes f._;ao9l . Appendix. EPA-821-B-01-004 and EPA-8.21-B-Ol.005. !JS Enviroorrieotal Ptotectio11 A(len% OH. _., " Page 6Bof90 .O*. a* Page 1 of6 Pobssium CJdotide: Chronic Test . Method IOOJU)) ,i . Species:* . Piniephales primtlikis ' . Tem:Number: 14 :Stnck so g . .. . . . . .. p ** * DiSSolveso ... K.Olml4..Milli-0\vater.. i:t 15* 18. '983* 9S2 .igQ(t 1000* 1000 In.;.house .cilltill'e , . .. .. .. : b\O\ .*:pB>= .. LS-.\ lliL .. 1Jii,ilyfeeilil!g ondrenft1Jiaf iiJfiJ111iation: 'J *4 ', 7,* Page 69 of90 li m.* 1099. * Iifoi1bator number and .* sbeJftoeation: D,rymg l1tfomu1tionfor weight : d1itf!n1iftiatf on: Date/.Time in c>ven: :. Initial *oven temperature: Date / 1iime oU:t or oven::. 'final QV,enJerri eraiure: Total:diylngJime: 'i .. Test. ir1itiatiori, tenewal, or termination ' *<::hV MHS\Y batch used SOP ATil=ExhibitAT2U,revision 11-01-14 l 1 I l I : ) .. Species: Pimeplzales promelas Day l 2 3 4 s A = Pan weiglit (mg)a . Tray color code:: _I q VCo dil Analyst: J1'-.. Date: "-l:;?x').\!11 . C = Larvae weight (mg) = B -A Hand calcuL'!ted. _ 1 Analyst: __ 11 Survival and Growtli Dma I J K .. L. IQ* 10 JO 10 JO to to io io *1' ** in 10 . lO* .Jb ( 0 ' (Q l t> , I .t>. . ft) ; . ( {) lO* t 0 .. IQ . IQ (C (Q .. /'Q t*o* tp . LC.* tq * .ltj lO* liJ. JQ, IO ., *lb to to lQ* lO 10 l(:) IO 'l0; ... ; d.() Jo. ,Q, l(:). fO. (0 ltl* I(.): lb :lt) .t\l JO lO ro l () ;t3.ol s.63 t&.S\ J1,,.ti. J3.11 ls.le tts.43 * * > " Comment codes: c-= =dead, fg. 'le = = ilii$.sing;, ,.;,, smalt lg = unusually large, d&r == decanted.artq returned, w =:wounded. Comments: Page 70of90 .., .. 0 J \ Page 3 of6 .Species:* Ppf<CJCR Test Number: 14 J:' . .* c=r.ame-weigJir<miJ>"".B,....A Haild , . . * .J ... Average . Pcrcecnt SimvivtJl altd Growth DUia 900 lllP KCl/L *.Q :R: .. * ... s* T u v w x *10 JO to: to l() JO m io 10 10 10 lO: *. ro (,'Q: JO 'rt) ; ' t b *-ll:)* 10 . ,. lO ; tO : -t {) -' I ti: f.D ID f () to' *iO IP "19 lO 'l;O*':{;O' gaJ . ..... ***-**** .. -*-u. 1 ., ls> JO 10:: -6 e -:r-r-....... (p .*. if.); l () ... J;. b b ; .. 1'f fqlf1 l'l,1 J1J!.f 1g.£lq , " ' : . iltiniber l)(lnr'v:lc (r,llg)' *ri:ifoction* i trom contr0l o. u .. S\."\ l {%). . . Con111iditS:. ' .. ** , ... Page 71 of90 SbP* Ari] -Exhibit A T2 J revision J 1-01-14 Species: Pinzepflalu promelas tl 2 3 ,4 n '= Pan + Larvaeweight (mg)* An;ilyst: Alb . Date: fl&-11-li c =Larvae weight (mg) ""B..,. A lfaod* calculated.- .. Analyst: Weigflt.pcr number. oflarvitc {mg) =CI Initial number oflarvae Hand caku)aled. xl .. Analyst: __ __ _,_,lP----- Average weight per Percent number of lll.m1e (mg) .rcduc'tion from contr:(!l .(%) ' . JOSO:ni Keitt Y Z ' ' AA * .BB , 10 lO lO 10 *iA.. & g1A. 'ti\. b ' ..., "G\ ,. ' g Sig '"' g* *fl;* 0 0 '.\ lo 11* .*,al 31. *tti ' ' r;. .. -' \ "' '1.. \ ' ls.6'-/ I s:Jt> Jfi.tf\. IS.b"': Comment codes:-c = d = deaQ. fg:=; J.ilngll$;k*= lolled, .m = = $irik. sm = unusually !:lmal, *.. lg*= unusuaUy large, d&r=decal'_ltedand renrffied, w=*wounded. Comments: Page 72 of90 Pag¢4of6 . . . .. Pimepha/es prom.e/Qs C:hroolc Refen1nce l\l'li!thod 1000,i);: .Quality COnfl'<il. V!!rlfl.Cl!tlon of Data Entry, iincfStatlstlcai _______ ..,.... __ __.'4_ EtMronroenfal'restlng Sollltlon$, Im:; T1!$t ifQ11s:. _______ ._11_u_gils_**_1_0J.._o_* ... 2_0_1_&.* 11* Coiltri>l .. *B. c *o E* !loo F G H. ... )_ K l M. 600 N <> p Q 750 R s T u V: w x y IOSO 'l 1111 118 MSO vaiue: PMSDt * a,.h'f'l.*.laf'ffa 1it¥11 crifffldanfdfvirl.at)Mt Mo!'.w**lf!i/JnltflJ hmn;Ha1h1tif4nhom hA ' .. . . mr . 10 10 .13.0l' 10. 10* 1S*.S!'. 10 10 .15,51 10 .. :10 .. 16;12* .. .10 10.: .. ,)5;96' let .!O 13,3!1 10 *10 . 14.49' 10 10* lS:lB* 10 10* *.1S.4o* 10 10. JS.17* .10 10 !6::U. .io* 10 JG.43' lO' 10-1s:oo:* 111* '10* 15:78 *10 *10* . 15.73 10. 10 "1S.9J: *10. '9' '15.14 10. 10* -16.44* 111 10 15.28 10: 8-16,23-10 6' 1G:4S, io* G. 1s.os*. 10 s !4.J9 .llL G l6':lG* .. 1a r 1s.s4:* 10 15.EQ 10* l. 16.09 10 . *is:o1: 0.0414. MSOo 11.J; PMSD" *!Wftlht1Jitnu1 ffl). fmll 18.87**. S;M 0,584 *o.584: Zo.9,S SA2 '0S42 0$1p u. 0.542' 100.0 o.na M Not 2lil3 . S,82 *. *o.se2 .. 2i;l!4 'g.572, o;sn* . . 21'41' SAS, .... .O.S45. 0.545. 18.85-; *SAG O.S4& i.8 .. O.S4G. 1o0.il 0.546 2.l'i* 19.77* .s,2s *o.S2S 0.!;1!6; *o.s.w. 4.2 21.43,; s.65 *0.S&S O.S6S* 20.n .4,71 0.471 0.471 *20.ss .. s . .a-0541 !i.53! 8.0 0.541 .. o.sn* 2l.S6 S.64 O.SG<I D<M u.o: '&.$ 21.99 S:S6 o.ssG .. o;sss:: .19.$0 4.60 IM6(f OADO. 20.19 4.41-6,6 'D.441. 100.0* Mn M* 17.2 20.ee. S.1S O.SlS o.4n 1'.Sl.S 20.64 4,72 0.472. o.472* . 19.9$: 4.81. O.Sl4 0.481. 2.t.54*" s.io o.s10, 0.518 8.l li.S10 92,5. *0,477 .S'.J '16.4 *!JI.Ill 4,54 0.464 o.464 4-51. . 0.564 0.451* .. .n* 0.457 0;27-J '17.97 Z.S!' o.4al 0.4.80. 10.7 IJ;l$;-51.!i !UR 4.6 17.!4, .2.75 0.550 0.275 .18.94' 2.sa*-,o,430'* o;:>.SB 16.4S 0.61' *o.s10 0.061* .16.78 .. : ... us:.* ;0.590 *05?3 O;Ua .. ns 48,G i1:ss* l.56 . 0;520. 6.7. 0:156* B;t;D* .*1s:G4 O.S7 *0.570 D;OS7 Mlnimum_s11niffcani Minimum Dltlereoce PMSb lj motiu(t of la1t p,..tslon, ihe_ PMSO fl lhb minimum peKtnl dlfferen{e '1e!Vleen !he oontrol.*nd 11 .. tmenl lhat.Clln b& d<<ll.W In a wh<lle cfflUeril w><iCity L!tiL LcwerPM5b bouf\d.d*larmlri6d l:tl. USEP11.l10th fH!m!llllll!I "121l. Upper bllllnd de!ermlnl!d bi> us SM (SOth petcentllo) s 3W . . low*r up""r PMSO bGunds Wl!fe determined from ihe 101h ond 90th respetilv*Jv, of PM5Dd1t1 from. EPA's WET lrtlttla bor1tory Vanabiltt1.5tudv (USE PA; WO la; USEPA, 2001bl. USEPA. 2001,, 200lb. Final Report: lntorl*borotorvVariabllflY StudyoHPAShort-term Chronk; and.Acu!o Whol1 EfnU.n!'fo>diltyTe*t Mothods, Voltimes EPf\>ll21*Mll*Ol!.4 nd EPA'321-/Hll.Co.5. us Environmental Proletllon Agency, CJnclnnall, OH. .... ,. .... ) s1ar1:oate: End Date: 819/2016. sample Date: * comments: Conc*mg/L D-Control 300 450 600 750 900. 1050 1.0000 *Loocio UlOOO 1.0000 o:sooo 0;6-000 0.1000 LarvalJFish Growth,and survival Test-7 Day survival* ppl<CICR: . .. Sa111ple,!t>; . 4b IP: :ET$*Enlllr .. 1'estlng sot. Sarr;p!e lype; t<CL;-P.otasslum ehloride. firotoc:OI; FwCHl'M!PA"821*R"02-0'i°3 PP*.Pirile?.hales:prert)efas; 1.0000* .) .. 0000 1*. 0000 :;.oooo 1.0000 *.1 .. 0000 1.0000 *:l.0000 1.0000 0.6000 : :o.sooo 0.2.000 '_0.3000 1.Q()Oo 1.0000 jJJQOO 1.0000. o-:ao?Q O.OOQO 0:1000* Conc;.mgtL N11111t1er Total Resp fllumi:itr D.,Conlrol 300' 450 600 750. "900 "1050 1.0000 1.0000 *1A1;io 1.4'12(1* 1A12p* .q;ooo. 4 1.0000, 1;4120. 1*:4fW 1'4120 O:OOO :\l 1.0000. 1.0000 1.412'> 1;4120 1.4120 o.ooo 4* 1,0000 1;0000 1;*;i:Zo 1A120 o;ooo. 4 0.9250 0.9250 1;295'1 1.1071 1A120 11.347 4 o.5750 o.s7so o..asos -o,78s4 <i.8sr:;1 5J!47 4: 0.1750 .0,1750 Cl.4217 0.321!;\' 0.5796 29-JiB:t 4 18.00 1iu:io 14;00 10;00 10.00 l(l'..00 Hi.()Q' 10.()f.)* 10.po. Q 40' ti 40 0 40 A 4o 3 40* 17 40 33 40' AUXIiiary Tests: . *Statistic .. Critical Skew.. Kurt * Shapiro-Wilk's Test non-n.oi:rnal 'Clistribution (p*'<"' O.Oi) 0.83328 .0.896 Eguallty of variance cannot be confirmed HyPothesis Test*t1,tal1; 0.05} . NOEC LQEC .. ChV .TU .steers Many-One Rank Test* . .750 900 Treatments vs D*Control Ma.)clmum Likellhood'*f'roblt Parameter* Value SE 95% FtduC!ar Limits eontrol. .mi1,.sg Crltlcai P*valutf. . M'u Sigma Slope. Intercept TSCR Point EC01 EC05 eC10 EC15 EC20 EC25 EC40 EC50 ECSO EC75 ecao EC85 EC90 EC95 EC99 16c4547 2.45609 O" Q:OSSS 9.48773* 0.99948 0;060.77** -43,?a.5 i2694s 1 s11.'os3 *z9.:;a5 * * * .. ** * * * .Problts mg/L 2:614 665;892 580.'9.01 719.902' s:35s 732'522 777.202 3.718 770.725 8?0'416 3.964 1:134.25 4.158 61S.563 i71.149 4.326 839-059 794.7:j'i 87;2.396 4.747 889.993 *853.898 923.$73 s.ooo e22.1i1 aa8.6s1 ss9.741 5.253 955.3138 /920.64' 5.674 1013.$8 97*2.105 1.077.81 5.642 10.37 .* 36 "1.11:1.5': s.o::i& 1066.-03 w1s;2s n5Z.a6 6262 1044,63. *1201;71 a.645 11s<F7 12s.s.o9 12713.91 1174;93 ;147s,4Q, 1.ll.--------'-'--------=----. o.9 D.B (l:7 . o,:.O.lr . .0;3 O,:!: o,1 . j '1' 10 too oooo Dose-Response Plot. *s. 0.9 *0.8 .. Q.7' > ea.a** :;, ell 0.5 Cl 0.4 "'*o.a 0.2: 0.1* o ..... 0 E (i o* 0 0 .,,. 0 "' .... 0 0 .. . Page 74 of90 ltet. 3' ... 'Sfart i!f212t1\5 'Eru:tl_)atii!. . StlmpleUale: .COmmei>?s: O'<lontror -0;5540 . $00'* 0)5450 4so 0,47.10 600 0.4600 7so OAil10 900* 0,;>;740 1oSO 0:5420 a:s46o (l,5419 .o.4410 o.s'ioo ,0,2590 .o:Heo StatistiCal Analyses 'Te!ltJO!'* sample 10: REF-Rer Toxlcanl L;ab(O:; Sot, saritpleTypi;: KCL.*Paiassium*ch101ti:1e P,Ptoeot Test Species; 'PP-f'imepbilles promelai; . 3. . 4 *o:se20 o,5720 0,5550 tt5640 0.556il 0.5150 0.4720 .0.4640 . 0;4510 0:21so . o.2sao. 0, 1580 !M)5/'b *rrariSfOfrll::Untr.:instol'l!'lad* '1-T:iilac:I. Isotonic cc:inc;stlt . llilDi:ln 'N.iMean *Mean Min* Ma" CV% .. 'N .. t-SW *p.qonlf'Ol 0$(00 '1*.0000 0,5700 oo;S4ZD* 0,!ig.(O :3A02 *r '300.* ct54eo 11;9579 !Mi280 0.5550 '2:770 4 1.'194 o.s33o 0.9351 tt533o 0,4710 9.5i>40 7.-958 4 1.84i "fjQO 0.'4726 0.412:0 OA4iO* *o.s1so 5.6.ia 4 4.a77 ..,50 U.4765 O.B36tf 0.4'1'65 0.4510* 0,5100 s.349 4 4;653* son '0,4e07 02740 1).2580 0,21!90 *t626 4 '!i:lso D.0980 tf:171,9 b.o&so *o.n57o 0.1/360 48 .. 631 -a Avi<llia!!Toms* Stalistil:' CP > 0.01} 0;97576 Baril£.lf.sTest lm:!ieates.e9uat vanancii!s:!e .. o.s1I .3.Zls4s liYi>QtbesisTcistf1*tail,O.Oof** Ourinett's Test* 0-Cooliol . mgiL. SD NOEC *,l.OEC. ChV:.* TU. .MSou*. MSDe 450 *:eoo 519,!>15* 0.!)474'3 0.063:£ Unear lnterpolallon {200' 95% CLISXPL .. Skew *... CiitiC31 .MSD Mean 2.'360 0,0474 9,5100 ();5450 2:350 0.0474 0.5330 2;360 0.0474 0.4743 *2.3W 0.0474 OA74'3 0,2740 o.n9ao Critii:al Skew o.asa '"0;5272 13.2767 MSB MSS. . F.Prol:I O.l>Q754 0.000!11 *s.4E-04* ICQ!; 1c1c IG15 IC20 IC-40. lCsb 351.92* .513;e3 763';67 1e$.ri:Z S49o 891/re 92.SS* 46.as' 38.:97 125.98 -579,-:;3 0.0791 *1104.90 Stltl. f3 459.54 . fiS.4.<!3 484,61 '7S1;91 '7ss;:1 -0;1aes stt1:36 o.oz.es ais.12 *004.es o.'1492. *1.CJ*,,,., -------,,--------. 4.82 0;9' o.fi* 0;7 ii: 0.6 c g_ ., 0!4*, a.a 0.0 0o=;::;..,.._,.....-,....,..,....-."- ...... D 1500 Dose* mgit. o.S =-. f0.4 C) . c .. (1.:2: !);1 Page 75 of90 14aa. *q.os 1ev:el of signll!cam:e N-Mean 1.0000 0;9579 0:9351 0.8320 0.8320 0.4607 0:1719 Kurt 0.54083* df 4,15 i ** } .J , ---.. __...! .J Species: .Pimep/iales promelas Daily Cltemis . . Anal' st Concentration Parameter pH (S1J,) DO(mg!L) Conductivity l mhoslcmi DO(mg/L) 300 mg KCIIL Conductivity { *mhos/cm} Temperature ("C) . pH DO (mg/L) 450 mg KCl/L Conductivity CP.mhos/cm) Temperature (0 C) DO(inglL} 600 mg KCllL Conductivity lOS/cni) *Temperature ("C} pl!(S.U.} DO (ing/I..} 750 mg KCl/L .Conductivity {µmhosfcm) Temperature {?C) pH(S.U.} DO(mg{L}._ 900 mg I<Cl/L conductivity ( mhos/Cirl) "Temperature {"C) .pH(S.U,} DO (mg/L) 1050 mg KCl/L Conductivity Otmhosfclri) *Temperature (°C) Page5 6f6 BpKCJCR Test Number: l4 "'Temperatures performed at the time of !<:st initi:ition, rene\v.tl .or tcmiinati911 by the analyst ideniified in tlu::D11ily Rcnew11! table loCl!led im I. , ... .;,...:, ity and hardness performed by !he,arialyst idcnlitied,on bend! sheet sp;:clficfor each aoalysis,and'transcn'bcd to this*beneh ,shect.Q:y; 11:.v1 ..... 1rv r..*"4rµ Page 76 of90 SOPADl -ExhibitAT2Li, revisip11 ll*01-l4 Cl ':> Q' ;; .. * ,,._. . ..,,.'"': i} Speeres: proifzelas Page 6 of6 PpKCJCR 14 Day each perfonned pH, .D.O. aild conductivity m¢aiiurcments only, i Concentration . CONTROL . p}l.{S;tJ,). QO.{mgtL) f mhos/cni). "*Alkaltoi!Y . <m CacO:ilL> {moCaCoJIL) C'C) rm (mg/Ll 7.9 300 mg l<:Cl/L .. ti'i_. 7*1*: 450mgKCllL 600 rngl<.Cl/L (°C) DO(mg/LJ Conductivity (pJTihostcm} Temperature; ("C) pa (S,l!,) . . D.O (mg{j:.l Conductivity mhostcm *, .;Tcmpl9'ature .*<aC} p.l,J{S.µ'.) .DQ (mg/(.). 750 mg KCIJL. , Conductivity 900 mgKCl!L 1050 rngKCJ/L "'Temperature . (°C) ,pH(S;O.) . PO{mg/L) ConductlVity cJimliOsl.em) t"C) .Pll(S.U;} J)(){mgli..) C<>nducfulity c rtiiiWcm> <°Ci *.. *. . foifial . .Final Jnitial Final Final lnitial Final . . , .. . . . ... . .. , ; . time' or t¢fminatiori analyst. identified !"'!he: table locdt on Page I. >;.llru!ll!fft!i perfonned by the analyst 1de11td'icq 0111he bench sheet *specdic for each :malysis:and transcnbcd co*thtS bcnch sheet by: . Page 77of90 . SdPA121-ExhibitAT21.l, revision'll-01-14 "" 'Ceriodaplinia dubia: Chronic Refet*epce Toxicant-Control Chart .... Environmental Testing SolQtions,.tru:. '1 --u = z ...:l -CID .._,, in l"4 u o= "'O I ...... 1.14 USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1.10 l.08 .. .. ,. *. . 1.06 l.04 *' . . *. . . . . ....... . . . . . . 1.02 2.5 USEPA Warning and Control Liniit$ (75 1 h and 9o'17 Perc_entile Cr's) 2.0 1.5 1.0 * * * .... . ..... * Ii e 0.5 --**"-.***-***.-* .... .. .. *--*-.:.-*-- ... .e: .................... !7 **** ,, ........... ..................... ,*:--*** 0 ... 4'0**it ....................... . 1.4 1.3 Warningct.ndControl Li1'i1its*(I0 1# ........................... Qll(f(J ******.* ,. " ............ ......... l.2 -* _., _____ ... :.--**- .. ___ ,,_*;--... -1.1 * * * . ......,, * :--0-*. * ......... =-.. .* * .............. , *** .. * .-==.* ** 1.0 0.9 7-day lC 25 = 25% inhibition An estimation. or the of'sodimn chloride* that would cause a 25% re(luctionin C?riodftPhnia reproduction for the 1 testpopuiatfott Central Tendency (meaii IC 2 s:) Warning Limits {mean IC 25 +/- SA.JO or SA 75) Control (mean IC 25 +/-SA.ls* S A.'90 , or2 Page 78 of90 2 :3 4 5 . 6* 1 8 !J* 11 12 13 14 15 16 17 18 l!l 20 05*03*1!'1 04*07*1S ilS*OHS OS.OMS ON<\*lS 08-114*.iS 011.iS*lS l0'06'1S 11'!)3-15 12,98-15 Dl*i,2*16 02*09*16 oi-ofa-.1& o4.os-1a U4*llS*16 QH0-16-b6'07-16 DHHG 08*0H6 7*dilV \Ch (f: s (g/l NaCl! 1.05 l.05 a.01 1,(Jri o.oi 1.0.1* 1.05 0:01 l.08 J..05 *o,Ol. i.07 *0.01 !;oli. i.01 .0.01 1.1i*1 1'07 0.\)1 1;06 l.07 '0.01 1.07 1;07 0.01 J.01 1.07 0;1)1 1.06. l.01 Q.Ol 1.06 1.07; O.ot 1,06 i.07* 1.0.1 i.07 M1 1;07 1.01 o.(lt., t.ciG 1.07 (teil 1;06 1.07 0.01 1.05 1:0& a:Ql 1;06 1;06 0.-01 1.07 l;06 O.Di-Cer(odaphnfC'I d.ubla Reference, Chatt State. and US EPA control. tT*:2S CH2S 1;04 l.08 1.04 1.0ii i.05 i,oa* i;o4-1.011. i.os. 1.05 1.oil 1.0S 1.Qa l.OS' 1.08 1;05 l*Oil* 1.05 1.08 l.OS 1,08 1.Q!\ 1;09' l.OH l.OS 1.08 l.OS :l.08 1.05 J:.()8 i.DS 1.08 1.0S foe t.9$ l;OQ, t.05 i:os SA<*** ll.O!l. 0;09 0,09; D.il9.* 0;09. ii.og MB o:o9 0.09 D *. 0.09 0;09 li.il9 0,09 0.09 l).i>ll to_fiotatary Watillng liin1rs er' .* . o.iJil *US p:r; 0.98' i;t5 0.98 1.JS o.911 0*!18* l.J:S. o.!ia us D.98 l.1$ 0.98 US* 1:15 0.9.ll 1,15: 9'.9!1 us ().911 1.15, 0.98 l.iS. 0;98 L1,s li.98 1,i5 0.98 1.lS 0.98 *1.1s D,98 US a.ill 0:11! 0.18 *0.1.s 0:18 0.18. *o,1s D.18 0.18 0.111 n.18 0;18 o.1s 0.18 0.18 0;111 o.111 0:1s 0.18 *laboratory cantro1 't1mns cr ct+ $,1.ii D.88 i.24:* 0.88 i.i4 l.i!i Q.88 l.is :o;as 1.2:!;' 0.118 .l,.l!i' 0.88 l.25 0.88 1.25 o.89 1.25 0.89 1.25 l.'l5 0.89 1.25 U,89 l.25, 1.2$ 0.88 1.25 0.88 1,25; 0.$8 l.25 ().88 l.24. 0.88 U4 0.88 1.24 USEPA* .s._ro <;T*SA:n cr+s,.,, 1 IMS D.$9 l,.5'4 o.66 0.48 D.59* 1.54 o.e!i o:-ta o.sii 1.54 0:66 .D:4ii* Cl.5!! 1.?4 0.66 o.s9 1 .. 0,65 0.48 o:sg l;SS. 0.66; OA!! Q.59 o,6$ o;4s P,59 1.54, 0,66 0.48 o,sg us o:&Ei !Mli 0.59 1;s!i 0:66 '0;49 *o,s9 1.s.s C).66 0.48 0;48 G.48. o.s9 l;SS 0.66 iJ.sg 1.s!f o.S9 1.ss o.66 Q.48 0.59 1.55 O,Gli 0;48 o;s9 1.55 0'48 1.54. o.&6 0;48 .0,59* 1.54 0.66 0.48 o .. 1.54 o.48 o;S9 U4 0.6& Nille: 1-d It;,;" 259' An*e&timat.lon of concentration of sodium chlorida that would c;usc *;, *2s%*roductlon In teprodui:tion for' th& . tenttal tendency (mean 11;,,j; $'" Standard or tile 91 o.40 1:72 0.01 MO 1.7i o:oi o:lio: 1.72. 6,01 9Ao, Pl 0.01 iMO.-.l.73 0.01 0,40' l.73 oJli Ml i;73 om: o . .;o: 1;n. 0;01 g.4l 1.7-3 O.Dl d.41 1.73 Mi ().41 t73 O.o1 0,41-1.73* O.Ol 9.41 I.73 Q.01 0.'41 i:J.3 .{J.4t l;'ll 0.01 0:40 1.73' t'l.01 OAO l.73 0.01 l.72 0 .. 01 0,40. t.n 0.01 MO. 1;72 0;01 ,.... , and Warnln11 tlmlts . . . .. . .. .. _, . . . . ,.,_,. .. _ . * . Laboratory .control-and. Woinlng limits wete es1abl11hed U$inil the standard deviation of.lhe lCn corrospondln8 to the lOtli and ::iSth percentile These end recon\lllended.by*ussM far the test ineiho'd Md 110dp0Jn1; s .. ,. = Staliililrd tho io** percerit!Je.i;:V,, (s.,,Q = o.oli)' s *. 11 = stondard deilia1ioncorrespo11dlng.ta thn 25" l'erceritilo CV. (S,.n = 0.111 USEPA Control and. war11Jna Limits s....,.: $timdar<f rlevtaticin correspondfng,to the 75** iiercentilc CV. (s,..,," o.<ISJ SMo"' Standard corre1pondlng .to the 90'" CV. (Sua" 0.62) r:*l= Coeffcclent of 1r.11iat1on of tile IC 15 values. IJSEPA, 2000. and Ateauntlng For Method VallilblJlty In Whole Elfluu.nl Applications Undet the Nat101ml Pollut*nt Oischa*B" Elimlngtlon Program,. EPA*ll33*1\*00-0IJ3. vs fnv1ronmenlal Prottctlo11 Aganty, i:lncinnatt,.OH, ,,1..--'f :;* Ceriodaplinia dubia, EnlllronmentaJTeStlng Solutioni;. Inc. Reference Toxicant Contttil Chart Preci&.ion of Endpoint Measurements 40 c:: 35 .=: -..... CJ = 30 -e e 0 .... --Q. bf) 25 c:: *r: *. ' ****** ............... ..... ...... 0 .... *'!*..-**'* ....... -Q. 0 20 ... -...... c 0 0 ._, u 15 .. -*.,,-..... -... .. Minimum Criteria(> iS.O offspting,per stirViving femaie) -'$. c:: 40 .. c ....._, *-c ..... .2 = ... ,.. __ ,;-.,.*----;***- .. .... ';-.. North Carolina .AcceptaneetJmit ( -"Cl 30 et! 0 .... .... :... = Q. > ... er:: 20 0 -c ...... :: = Kentu<;ky Acceptanee Limit ('S SO.fl%) * = .E:i 0 !,;: u 10 ....... i... 0 er= u 50 . . .. _,, _______ ., __ .. usei>A u;pe;PMso <90 1 b .. 40 -'!-30 -*10 Control ofVariatic>n (CV), or }Minjmuni (PMSD) PMSD is-the 9.etvre,en the c$;Jptr9] an.d treatmeD,t that can be declared statistically significant. .u. Central Tendency (meaJi ControlReproductiotJ:. CV. or PMSO) Control Limits (mean Control Reproduction, CY, or P.MSD Page 80 of90 Predsion of Endpoint Measurements Ceriodophnia dubia thronic:Refere*nce Toxitant Data. ' soiiltlonSj Jn<:. Tt?St Te$hfate Cont!'Ol .conti'.Oi q OJ er MSD PMSD CT number suniival Reproduction for Control Mean for Control (%) {offspritjg/fematel .flt:prOdui;tioh (%) Reptodliction (%) (offspring/female) .PJI%} l. 03"P3..:1s 10() 3Vi *s.n l.9 5.8 2 loo 32.3 32.4 4.6* s .. 3 1.8 5.S S.6 3 05-0s-1s* loQ ,31;7 32,1 5.6 5;4 1.& S.8 5,7 4 06-.09-J.5 10cr 29:7 31.S 2,g 4.8 1.4 4.8 5.5 5 100 30;8 31A :rn 4A 1.5 4.8 5.3 6 100 3,l.3 3.0 4;2 1.6. 5.0 5.3 7 09--)5""15 100 31.0 4.0 4.2 1.7 5.9 S.4 8 10*06-15 100 29,6 :30.9 4;8 4.2 1.8 . 6.1 5.5 9 lOQ 31.7 3LO f;;S 4.5 2.2 6.9 5.6 10 U-08-15 ,100 3\>.9 4,9 -4.S 1.9 6.3 5.7 11 ch'."12:.15 100 3i2 3o:9 3.3* 4.4 1.4 4.4 5.6 12 02JJ9.-16 100 31.:6 31;0 1.0* 4.6 2.() 6.3 5.6 13 02*P9*1Ei 100 32.0 31.i 5.9 4/7 i.6 5.1 5.6 l4 ioo* 29.4 31_.0 5.4 4$ 1,7 5.9 5.6 15 04-05-16' iQO 29 . .3. 30;8 :6,q: 4 .. 9 1.8 6.1 5.7 16 100 32.3 30:9 4 .. 9 4.9 1:1 5.2 5.6 17 OS-10-16 10(} 31.6 *31.0 5.0 4.9 1.6 5.2 5.6 18 06-07-"16 100 2.,7,9 30.8 2.6 4.7 1.7 6.Z S.6 19 . 07..,1_2::,l,6* 100* 3?..S 30.9 4.9 4.8 1.7 5.1 5.6 20 08'-02'-16' 100 29.9 30.8. 6;6 4.8 2.1 7.0 5.7 Note: tv: *toeffiCient ofvariatiof! ior controirepr0di.lttion;. CN bound determined by USEPA (10'h UpJ?ef. liSEPA percentliel = lli% MS_,<:* MiJUmo'm5ignifu:ant.Iiifferenee PJ1.11SD o::: P'erc¢Jli. PMsD ls a mea*sure oftii$t The PMSD is tfie minimum percent difference'between the control and treatment that can be det:fared.statisticallv Sigi-iificant fii a wtioJ£!eff1uent toxicltv*test;. . lower PMSD bouhd.d¢termined by USEPA(1o 1 h percen'tlle} = 13%. . Uppei"l'MSO bound d¢terrriined bv*t.lSEPA(90m percentile) "'47%. q '= CV, or PMSO) VSEPA; >2QOO. and Acc01.1nting fot'Metllod Variability in Whole Effluent Toxlt:ity AppJicatio!lS: Under the t,iaticioa!Pollutant Discharge Ellhiiriation Pf0granu EPA-83'3-cR.0()..093, us EnVironmeotal Protectiori'Agencv; Cincinnati, OH. USEPA; 2ooia>::zoulb;.1'lnarRepO'rt: VarJab!lity study i>fEPA shorHerin dtroni(:.and AcuteWli61e*EfFhsent Toxicity Test Methtids, Vo1t11nes l and 2*A'Ppendi11: EPA*Bii-a-01,004 arid Os Envkonmerital Protection Agency; Ondrmati; Oli. Page 81 ot90 l l I _.I J J 0 0 0 Sodii.m.Chlotide*Chronic . ! ... Cedo<i:apluzia d11/iia. Diluti<in preparation nifOTlll(ltitm: .-Comments: NaCl Stock !NSS number: .. *mss Stock preparation: 100 gNaCJJL:: . . Dissolve 50 it NaCl m 500 mL MUli-0. water. '*: Oilution oi'eo CimzJL) 600.* 800 1000 1200 *1400 stock volume (illL} 9 f2 'lS . :J8 . '"2J Diluent'volume (mL) . 1491 .... . .. 1488 148) l482 1479-Total volume{mL) 1500 :1'500* '1$00 1500 15.00. . "" ' Test orRaiilsm so11rce hi,fi:Jtmtilion: ' 1<!i'til,tformatio1t: Pate and times organisnts \\"erc'brim lticµbawr.nwul!crand'$elf between: * * .. .. ... Ctilture board: . \t... .*A number: . f , l .I . :t, r 4: s r '6* " 7 I *:s .. r !) l .JO. VWTbati;ll: Culture board cup number: . b.. Q h'.'1.. f'l\, Jg I "U I "Jli .11.=i Transfervesscl infonna!ion:* .. i:>H= ,ti .. Tem!leniture= "C.' Average iransfer volume fmL): * : IMl:inL Day 5 08.'-0J'-16 Cotttro/ i11formatiom %.ofMale Adults: % Adults having :;Id Broods:.,; % Mortality: Mean OffSp:ring/Female: %CV: ! Page82of90 . 'tl!St hiifuitijjn and-rene\v,i,tl n.d feJ:(iing, or* * *. termin:ition time J"" 61 .. <::40;0% MMSW ,f1atcf1.}fscd .LOEC *'* of.6 l l l l 1 --1 -1 I . J J -j ! -*' J 0 4 ;:I ,; I .J:. Page2 of6 Ceriotlanlttii'ttduhta c-ONTR.O'.L CdNaCICR#: 191 S;utvivat t1itiJ lleJlroductiim Datn. *. D.av .. :t *.. 2 . ;3 .. . . 4 5 . 6 . 7 8 9 10 * *1 * .* \'ouilg CY : * .0. 0 U 0 : 0 0 0 C) / t'1 ** * Adutti!tlirtalit;r \,...,.' \..... L *"-L . L. L L : L 2' Vo'qngprod.uuf) , . 0 : * . () . ** **** h: .* CJ ; 0. .D . 0 0 .O Adult mor.tafity . . . \.,..... * \._,., L. L L-*_\;....;. . \..,,.. ., . ,..._.; . *\._ l..._ s ... .0 o.. "".* -:""-. f'iif: * .. Ci : D:'. *O, .6 0 . :. L -\.-. \....,:.. L; \..-L. .L. .._. '-* 4* .. . q-. ,,_r. ' * . . : 'C\. *4 * *'""\ .* '..\ .*'L. *y L.. *6. * \;,;. L \-L.. L.-\...... *. . :f{). :, it!:l. ' t\ .. \ "t,.. .\,_ i'"t . \1.. l 0 I L5 Adult mortality L.;... ** ** \......;.; \;.....;._, L. L * \...-* (\...... :\....... '--C * ** . /:'\: n f'\. , Q () . M .. tn* . C 0 0 4* : . L,.;._. . \ .. . ' 'i-,. :L. * \.+.. .. . L . L . '-.. '-. F'llial:AduJtMonatitV ** * L...: '--'* '-* \....... i '\L... \..._ L:.c * '--Xfor,3'C!BrotHJS* . . ¥"-* ... ><-* ¥ * * .... X.. . r... *-,...;;. Q,._<=.:nve. o "*:dClid}; SB'::*".splitbtood(smgfe broO!hplit between CO =caey over (offspong * * * .,. .. ____ _.'..,. _. _______ .....,. * Concentiatihn: Meari .Offs rb1g!Female: '\ .. ru. .. 1' .2 .J 4, 5 ..6 1 .8 !) IO J Vp11ng .. ,6 l'"'\. ' 0 c l\ l'\. Cl. ,. 0 A 0 .... ..... . .. .. k '\.-L. .. \.;,_ , . L L, \ .... L-. .. L .2 produced ' 0 D i ... o 0 ... o Q 0 c .. C5 . . Adult L . 'L ) .... L. L. L -t.... L '-.. . J pt1>d1u:cd* ... r-... f\.. << !';'\ <<} . .. A . (\ c 0 a L ...... L-. *\.._.;:, . L L : L :.4 L . L L 4 Youllg ,q s .. .. *'4 . * . '*'{ H: ' 'i . . mo!'tlint.r..: .. * .. L L. L ;L \....., L.. ! L L L. L.. ,. : . ;; .v:oµng iwoihrced .. l"'l...-. .Jo l.\ Jc;} * : :\ i;,. .. .. 11.... *fl \ : 11 l,_ \\ Adlitt morfaJify 'L ' L *t...-\;,::... \..... L ' \..:-. L.. L 6 ... . f"':l 'h.* . 0 .h .A ("\ 0 0 0 0 *Adult'mortaJit,* \... L L \....,.. L L L L L L 1;. I Jp tS. '.'S lS' l'"' .tb _,, \'S l 'S -TO.t:t1:youngpr<iduceil-1..1:\. . 'Q\ '2:fL ot 3\ L!( /VD(e,.' J\dtilt Q.. =]ivC,. D brood split betWcerl two CO= c:arry over (offSpring '* * * * ... ,_ __________________ __ Co11c(mti'atioll: % Mortaliiv: 01. Mean Offsi:ldng/Female: Page83 of90 S()P rc:Vision ll-Oi-14 l I l .1 Species: Ceriodaplinia dubia 800 mg NaCl/L .Survival ali.d Reproditdio.11 J)irtq Day l Youag.produccf . Q, M. * () I'. ,C). *O * ('\,. 0 * .0' , Pl .Adlllt mortality* \._ (_ i:: *:L. L.,. : . \.:_, ' L C . L ' :*L Yo1111g .' .Q . . . () '(""\ . * * ./""\* * * "("\, ['i\. *C\ * :Q*. . . :C'\ Ai.lultmortaltiy \_.. \ .. .-L L .* \:;.,,..; . L \...,. L 3 Y11ungpr.oduccd * * (, ('\ :f"\ .A* ti '{':\ ** 6* .O l'\. , Adult m0rtalitY L, . '\,....:.. ... L. C "\....... .. L; \...;... :\..::... \..... \;.,.. Young produC:ed '°'. * * *'4 "-'* .. ,,_ . ::S ' C::::. * . * * *q Adilltmorlality L ... \..-\.....,,. ** _\.,,.., *\._ \..... L..: \:...,_.. L Voungproihiced \L. \j \\ * \1::1 ' \0 \\ ** * *f\, . *:G \\ Adult mortality. 'l \ * * "-* . **L:-, \;..,.. , \..,.... * ' \;;;.,,,,;: : \...-_* * You l.J.; n . o*. . :r*.1 .\:.. '}.:. C:l .. \'..J Adultmort.lntY L.:. *c * \. ( "' t. .\. 7 Ymrng prod0cCd: \ ; * (-*t,p . ** lL.i . : \ \.;g* ' \ \..\ : \lp . l'\..\ * *Tota{yo!ing.produced 2 Q . * "L'\ '31... l .l . 21\ * .. 1:.1;* FinaJAdultwtortality . '\ * \..:. L... L L \;.... ... \;;...;_ : : L. Note: Adul.l!,llOitality carried ovenvillt .adult dUring liiinsfur).. * . * .. . * . . . ' * &,ru:e1ttn1ii0.a: ; * . .Meifu Offs o . . . . . . . .;b : .. 100() mg N'aO/L Rcnlkntc nilmber ** Day I 2 Yooogpraduc,cd ,(') * .(\ *** b Adultmorialiiy L L.:. L 3 Youngproduccd .. h () **("\* Adult mortall!Y l.. \,.;;. \_.. *4 Youngproduccd .* i-****c.J. . *\.:r **: Adult moriali,ty \........ \.:...;..: * 5 ll. 1 o .**. J.'.?S i o. '.. 10 :. * ... tb. *. u . Adult mortlllify .* L ** * *L * *L; 6 .o ; .h. * *. G ... ***_ o , .. 0 .. -l1 .., Young produced ,. t'S.: . I\: Total young produced . . .* *-i: '-* i ; FinalAdultMort:llity . : L.: . * \..._;.. ... '--* . L '*\;....; ... """-'* \..: Nore: Adlilt mortality (L ""live., D =dead),. SB,.. splidl;ood (s)n8l1!'broo!l sp!itbetweent\vo carried*ovenvitb adultditrlng , * * * * * * * * 0 l Meafi . ' .* 4& *. 1 * *%Reduction 'lfoin Page84of90 SQP ATi4' .,,,,µJiil:iit n-:OJ:t 4 l ; O" ;.-:<. *1 l l l *1 ( -I l . r 1 I . ! l I .) .... '* I _, Page4 of6 _Ceriollapkniddtlbia . CdNaCJCR #: 191 .. S'i!>'vtvt;i. n1fiilliProilu,ctiOn bat.a . num:be,r Day' f'f '2 1* 3: **. t. 4 J . s I 6 l 1 I. 8. I 9 .I 10 1 - ---o.-* -o cc---o * o. . . a ... a. c o A'1ulhnortiiliiY .* *\...;.. ' L L L \...,. ... \.....;,. \..--. \,_. <z -h _:. t\-o-:--Fi . -a Ii o Adfilt1'1t>rtt11ity \,;... , b 'L.. L *\._.. ., L . *L.. .*L. , \.-: \..... * * Q. : O. . *O *** D 0 *. :(:) : :!'"'\, . A 0 0 1 . L...-.. L . L.. * \,-. " \.,.;i. . ;.1-. * \.....,. : \........ i * * .. * *4. * * .. A* Z "L L , \......_ .. ,\.....; . '..J.... ... . L * \..... 'L. \-.. t.l*2--Jt.?**r-.tJ c 1 c. ,. . :l"cmngpr.oauia!d' .:D A * *o o ,r:; .. f\,, : *a o. -o * .f"I * .* . \._;.. e:** *L,.. \ ... ..J G L; '-\..... L '-. 7 * . . .:5 * "".L q. '°'.'\ : * \o . L.\ u . ,_ _ 1. J 1 l i4J .,4 l : I t o:f i I I . 'i I Lk L-I Lt I *c.*1 C . N014:r Aduft'm!>ftality, :(L-= live, n= SB'.= split fiioo4 t)ver(offspi'ing Cltrried' 4urtng"'91Sfer}, * * * * * . . . * * * * . <<:onf.iimtfittiQrt:, % Mortaiicy;. Mehri Offspring/f'emate: from Control: 140.Q.m_gNa;Ol!;, .. *. .$ziruJ1ml"(lnil /;)Ma Rcnlii:nfe number J>ay l :L 1 J .. 3 I 4 J .5 I -6 I. 1 J 8 1 Youmi **_.-,..,...*.**I . 'A;.I: *J':> I ""' I *A. I* I ,.... . . t I .L,J .. .LJ, U_ . t J , . L..> .... l/.:.J. f;_J J L,,.I * 'l:.-1 J, L. F \..;,.. J L LL IL a' Wimgpr9ducet1 1* !"*: 1 -1**********:r..*-:1** -Q .. O******** .. , ........ **Yo'\ **1: Q* . . .. , . r .. u ..... u .... ,_ -.... , ...... *"*** ...... ,, . .. \..,...-14 l: L J . L,.,.J ::c.1 '-* . 0 .. . .. L. *4 * : _ youngprodliccd 0 .. * .* . L. :L . L "S I ... * ... *"'.L I . O . . ** * * .. 'L.. . 1 . "'--* L . L \,,..,. * ""-.. . 4 L :.u o: I. 'f) I 0 .Cl J * .. I (). I :n Aaut(niorbilitYj --c::r-t. .... J 'L -i ,. L l'L. '7 1. l .: .:1-..1. ti :1 . \ ... I. 2> .. L 1... I 6 I -D I 14 J l . J 1-l I *I . '-t I \.\ . I ' 01. n .. \ Co'l.'\£. . 9 .('J L 0 \..... _Q_ \.._. 2 L 0 \.... n .t""i "1... lC) . r-. L.. -(5 L. .Q._ \.....,. .!L L '-( \.... .o l._ 0 1{ I C::l \. ..... " r.'L,..;_ I .'\o.;,. . J . L. 'I '"L. .I'\;-I .L "]-\..... \. ,Nole: cr.*=- tfe!id); l!_ttiod {single l>fi:\ad.'spli,t betwet:JJ'twodays); C0"" (OfJSpriilg * * * * * Coiicenlratl<>:'#:. Mean Offsptingj'Female: % ReduytiQn frontControl: . Page 85 of 90 .SOP revis'ion 11-0 l-14 'g Co C> () """' co <::> _ .. ,,.... ... -..... --.-. ..,... .) ) Verificatfon.of Ceriod"plt11i<t Reproduction Totals Control lODO mg* NaCl/L 0 Replicate number. 'ft>liil II)' I 2. J. 4 5 6 7 8 9 10 . Dily l 2 J 1 , 0 0 *O . 0 0 *, 0 0 * *O .0 .0 .. r ... ' 0 . 0 ' 0 2 *o . O* 0:.' 0 " 0 . 0. *O. . 0 () -.0 0 *O 0 .. 0 J. ..o _ o* .o : o o o _ *o o o o . _ o ..... ,3 o-0. ---* 0 4-" 4 4 _3 -,J s -*3 ; 4,. 4 4 ' 3: ' 17 4. . 4 '_J -i4 5 --* **-* IO .. . lL _ 10: 12. J2 9 .. 12 . IO:'. ... 10 -l<l6 .. ... s:* ..... 12. IO,-. rn .G. -.0 .. JL' O* . 0 0 0. .0. 0 0 ! 0 -. :o -:Ii* '** 0 -.o.,-_(I 1 . J3 -J7.. rn.. M. 16 1.7 17 U 1.S.. l6 156 7 'IS .. l5-.. 14" -.1*otiil 1.7. Jl. 30 27., 33* J:i 30 .3J 2:9.i ,,29*-299 -:rotnr. .Jl .28 28 -600; mg NaCJlL --,120Q mg NaOJ/L 011y *n.cntlcntc*llunlber* 1'ot,111 . l -.2 .-.J-i $. G -'7* 8 ' !> -* ljl.' ; I . *'2** 3. . 1 0 *o** . 0 *o o. o: *o .. 0 : **o** o: 'ff t <O . o*. . o* . 2 -.o 0 *O -*. _ 0 ... 0 0 o_ 0 *o ir *O .:2. :0 J) , .0 J .Q*' .Q (f -() *. *O . .O* () : . 0 . ....*. o*.**_ *_Jt ... *O ... J Q, 0 *O* 4 4 .. 4' ' '5, -'3 4 : ii. 4 5. .3: ... 3 .. -:19 -'jf :3 ' *3 c2.' 5 12 . H> * . u. ; 1.0 ' -12 . 12 .. -ll .II. . 13 ll JlS 5* 6. . 4*.** 7 *-6 0 o* *o: ... , JL: .. 0 0 0 ::o. . v o:: .-U. -ii. --o-0 . : O' 7; Ip 15 15 ' 18 ., 11.*: 15 T'7 -lS : lS 'ts' __ JSS ,, . .5 . i 4 Total * * ... 3%: 29:'.*, .3:1. :.31 J3 Jt ;-32" 31 *. .:n . *29* 310 *1 fohil *14 i 9* 1.3.' .... ,, .... , . 1400 mgNaCllL 1tttilicnti.i'iii1mlit!r -:--- -*-**-4> -*S': * -6; .. : th '9 -; '10>> Toti1i -Dn)r 1 *2 -3, Uily lJ 21*3 -.I. .O.l 0 l 0 *.tJ" ' '{) *o . '*{), 1 0: 0: tF -'* .Q *" ... 'r o* O' -:o--l l o -0 : __ :ir*, ' 0: . () '(.! :; -(I --(),. ... ::lL ... *2 'ff". *:o ... -:o. .****'J. o.J 0 I O', O< ' ll .-. (f. 0. -0 . ' 0 . -O.: ,() .l . o .. *O *,o**: ---*-****4 rrs:].4: 4 :'3. -:3 . 5-,,A*: -4, 4, .. 39"' 4 ... -_-*. ::1L* '(l, '_] s -12 J J2J J2 .n. ,J3* lO JI 11 -*13* ' JI-1'1'.6. .:5* 2 l' 0, .. ---i'i .o l'it I o _ O* .,. O' . .. o* '() **o i) *o .. , .:o ;ti ... 0 .--() 0 . ... 1 15 *t l:l J 16 J4. *'16 . IS< 'JS 14 Hi.* IA: JS! . Q; ...... 'tutnr*;.rlif r 31lJ .az :* ;2l)'. J2' .28:, -34, Z9' 33' ',2l>>: ' 3_06 *' *..iro1111. .4 --.. .1.. :..:.L ncolit'ntc number* Ti1tal .4 5 6 7 8 9 lO * .O* 0 0 . O* _* o-.0 -O' 0 . -0. 0 -0, -, o .... .. 0 JJ 0 *O 0 0 :O;. 0 0 0 0 0 5 ,4_ -.. ,:,i* A*_ 3_ J. 4-38 . I) Hr* *. [3 -JO* .. IO ,, -107 0 ' o_. .. Qi; o* O* -0 I} . J): 16 J3, Iii l:l 13 .16 l.3 142 *--* -32 21 '3.l" 'f).7 :26 ' :2!J 28. " 281-_ H.imlii:nte tll1mbilr*' T9r1,1.i 4 ' -5 6 ' ,, 8. 9*; (@;* .. --o -0 . 0 O* -o*. --.0 ' 0 0 0 .0 . .o. 0 -0. O' -0 0 ' 0 ' o* .i) o. .. l)* lL 0 0. .-J. 3* 3 l* '.2', .2; .l -.. 24 .... *3 ' 6 .6: .. "5.' .. z; 2 -, 5 46 -,0. ..0.' ' .(f . o* -0 :, l} ' -:o* () "l S. ::6'. 4 :* 4* ** 1' . '2 4-1-13* 14' *is. lfl . II (i -9 lU ite1111cl\ic. mil'libcr -4 s ' 6' .,,:** . lf. -.9 Tpl11l rn* 0 0 *o:' -*ll'.: . '0: -* -_Q* ... O.* 0 ' .. *o . o: 0: .* -0 l)_ 0 .*.. --() _o* .0 .. *O .. *.O*. !l -:'O:.: Q *0. 2.-. 0 ' L .. ]t j),: '2 0 JC _Q .. 'J. 0 :2. -:r *() .* 4 J3 IY * :, '!} __ :y * ()' i 0* -:o* *-o t 3 ; . ':2:* o-** 2. 'Jl :' ()* ;o tq** :5 _..., *_ 4-T :2 4 Jf' /: '!' ... ........... ) testing .Soiutlpns, Inc. Ciiliccmracio.11 (mg/I, NnCI) :? 3. Coufrol 27 31 30 .600. 32 29 3l soil 30 :3'0 n HiOO 31 28* 28 1200 14 9 iJ 14110 4 *2 l>11i111c1t 1 s'MSO value: 2.JOl }'MSQ: .. . --M Ceriodapl1!tia tit1bH1 Obr9nic l'oxical)t ltPA-s21,,,R .. Q.1uilicy Control of l)at? Entry, and Statistical Anlllyses ,f 27 31 29 32 i3 . s MSD .. .PMSD=. Test 1111mbcl'I . CdN11CICR: 191 ... Tlist dnfos: A11g11st 02*1!9, '2(116. lh:nlie11tc Jii1mber .Avcrug_e*rejwqdlii;tbiir* C<ieflii:ient uf l'C:rciml lton1 s (;-7 8 *9. to tol'fSpri11glfc11la fo) ('(.)' c0fl1rol (%)' 33* .32-30 31 19 29 100 2M* 6.li Not*appiic11illc 33 3.l 32 3l 31 2\) lQO .Ji.I)' 4.U .J.7 32 28 34 29 33 29 ioo 30.6 6*6 -:u 27 3i 27 26 29 28 100 23;7 7,t) 4,ii 14 15 ro s 6 *9 IOO IU 27.7 5 . 4 '4 2 4 IOO )> 48.4 Mittiini1m Sig11ilic11111 Di£lcrente . Pcri:c111Mlriim11m Sig11i!Jcm11.Qiff(lrcncc ..... ,._ ., ____ , .. , __ ,,_ *. l;MSD is n n'iclistm: of test precision .. Ti1e PMSO is. the 11\inimum perCl!nt di!Tcrencc bclwccn the control. 11nd treauncnt thot. cnn bc*dcclari:d sinlisticnlly significnill inn wlioie cffiuent Loxicity*tt:St. towefpMSDbound dctt:rmincd by USEPA (10°1 perccndlc}"'13%. Upper PMSD bound determined by USEPA (90 1 h percent!ll::)= 47%. Lower and upper PMSD bou.nds dctcmlincd from the 10th and 90th of PMSD dnta from EPA's WET lnterlaborntory Variability Study (USEPA, 2.00 I a; US EPA, 2001 b); USEPA. 200 In. 200Jb. Finni Report: lnterlaborntoiy Vnriohility Study of EPA ShOrt*tcrm Chronic 11nd Acute Whole Effiucnt Toxicity Tes.1 Mcihods, Volumes I* untl 2-Appcndix; EPA-82 l*B*Ol *004 nnd EPA-821-B*O I *005, US 1!11vironmcn111l Protection Agency, Cinoinnu1i, OH. Stan Date: s12t.201s End Datil': a19j201 s Sample Dale: Comments: conc-mg/L 600 800 1000 1200 1400 27.000* 32.000 30;000 .31,000 l4.000 4.-000 2 31.000 2e-.ooo :ao:ooo 28.000 9,000 1 .. QOO Statistical Analyses *ceriodaphnla**suritlVal*and Reproducti.on* Test-i;teproduction .. Tesno: *CdNaC!CR . .... .. . Sample.ID: REF.;RefTox\canl' .LablO; leslin9 .Sol,. Sample Typei c;ilio.rl.de Protoeol: F\iifCl;!R-!;PA-8?1-R,02-01 s Test Species: dubia 3 *3Q,OOO 31.000. 32.000 28,000 13.000 **+oao *21.000 31.000 29;000 32.000 13:000 !;:oop 33.IJOO. 33,000 32,ooo v.oo.o '14.-000 32.j)OO 31.000 28.000 31,000 1{;\000 4.Qoo 7 *sp,ooo 32,00ll 34;000' 27.000* 10,000 i;;OOil' ., 8 31.0.00 31.00P ,zs;ooo 2s.ooo 1.000 9 2!:1;00.0* 31;000 is;ooo ti;coo *2,000 28.000 . 9.0.00 4.QOO. Transform: Untransf'arined* 1'-Tailed ISOtimie Coni:-mg/L Mean* N;Meail Mean *Min, Max. CV% N Crit<<:a:1 Msc Mean * * . D-Control 29:900 *1,0000* 29.900 ,;mooo .33,000* 600 31.000 2s:oo.o 33.0.0(l' 800 30.600 1,0234 30,600 1000 2a:wo 0,9599 28.700 2s.ooo 1200 11.iOO 0,3112: '.11.100 6;000 1s;ooo *1400 3.200 0.1070 '.32110. s;qaa* -. Auxillary Tests l<oJmogorov D Tesl'indic;ltEis norl'llal dlstrjblltion: (p.> 0,01) Bartlett's Test indicates eoual .vaciMces {p ,,,.0.15)' Hypothesis Test ('Mall, 0.05) .... 'LOEC CliV Donnell's Tes! 1000 1200 1D1:J5.45 Treat1'11e11tsNs D'COlltrol s.;Sas Hl -04,023 1'0 -1.197 6.572 10 ti,978 10 1;300: *21.s10 10 20:41>3 48.412 10 29;(!62. statiStlC a;o1111, TU. MSDu* .MSOp. 2:10oe4: *0;!>7926 Linear Jnterf)o!atiQil*@lf! Resal'!lP!eS), Point .mgfL 969.444 1014.2 so . . .. 95% 'CL .. ..Skew .30.500 2,2a1 2.101 22a7 2:H>1 *30.500. 2.287 ,z;jj)1 :2a:'700 2.2a7 :2'101 2,287 a.200 .Cl'itit:al Skew* 't.Q35* *Q;064G 1s:ci$sa .MSB. .. MSE F..Prob* 1466;94: '9,3E,40 '!COS IC10 IC15 IC20 IC25 IC40 IC50 . 1031.53 1048.8'5 1013!3.19 1.11.S.18 1152.84 46.6e<>S '857;991 . 11)10;6 .,0.6536 8.37526 s,57571 101e.s9 1o#.2s: o.osso S.1'725 1036,55 1060 .. 77 0.1788 s;s422s 1os<1.1s 1ons1 0:2398 6,40685 1104.94 11?1.,2 0.2308 7.5251 t13'7.93 1169 0:1919 Q.1l o.e* o.7 ... o,s: ... . g o.s i oA ., . a; o.-3 0.2 Ct! o.o ,.6. ....... ,... *. _,,,. _______ .o.-"--<6' -0;( >jo..,,_,,-.-_,_ __ ._......,.,_.......,... ..... --1 nose-Response Plot 40 ...----_,,.,.----,------.,.------..,., ,g 25 .,. ,s:20 e* *c. ./}. 15 10 Page 88 of90 500 ooserogfL 1.ftiill; o.os tevet 1,500. '1J>OOO {oaoo ,,,001)0 O;S4,1Q Q.3639 Kiln -CJ..S:499 df. J?,Sf l l l l l l l _I I I g r: Concentratfon CONTROL 600 nig NaCl/L $00.mg .1000.mgNaCtlL x200 mgJ'faCllL '" Analyst :Nranieter pH(S.U.) I>Otmg!L) ColJ()uctiVity µmhos/cm) '*AlkalinitY ( CaC0;1L) *Hardness CaC03'L) Conducllvity { mhos/cm) . :"Temperature, (°C} pH(S.U.} ' GoniluctMty ( hosicm} .. . ("C) pH(S,U.) *Conductivity fttmhos/cml .. *Temperature ("c) pH ($.U.) necmg(L) 1400 mg Na.CUL . ConductiVity << os/cm} *'femperature.("C) .. Page 5 of6 CdNaCICR #: 191 Jl'.llrf;oi'riled at the time ofti:St. foiiiatipn, renewal. nr lerininiitfo1t by lhc analyst i_deiitiried m the Daily Renewal InrOiJJUl. rabklocat. .cd on L Alk.*alinity anritess perfom1ed by the anrifysl identified on 1be beitch sheet specific for each analysis and in'Jnsctillcd to this !Jencltsheet:by: . , Page 89 of90 SOPATl4--EXhibitATl4.1, revision i 1-01-14 -i l -1 ;fik:J r: ) Species; Ceriodap/mia dubiii CdNaClC!t#: 191 1------------------------------------- 1 .Analyst 1-----------...------------ Concentration P a ram et er l DO(ll'ig/L) l CONTROL Conductivity ( mhos/cm) Albli.iJity {mo CaC03/L) 1 (m° CaCO.i/L) {Anal !St.identified foreiich da l *Temperature ._ __________ __ ....., __ _.,,.._......., ....... ...... .... .....,_......,_. __ .._11----.._..-- ...... 600 mg NaCJ/L 800 mgNaCUL pH(S.U.) DO {aiglL) Conductivity mhoslcm). DO (mg/L} 7.g Conductivity }830 C hos/cm) *Temperature (°C) 1 pH (S,U.} 7.fO . . no (mg/L) . '7."l 1000 mg NaCJ/L . "'I "'I. .J* *.{) { mhoslcm) ""et l . "1>\.<o .. .... .I DO (mg/L) 0 . 1400 mg NaCllL Coqdoclivity S>. 6 i 6 ( mhos/cm) .. lq Initial Final 'Firial Initial ***:ii'inai hdtiaJ *Temperatures perfonned tirm::0f 1t:st* initiation, renewal ortenninatiQo by the aMI;iSlide!ltiliC,!:in the Daily'. lab!e lo::ated on .I, Al. klil. antrdn'e$spedonned b:Y lite analystiilelitified foreacb anal}'SIS .and transcnhi:d to tins heilch sheet by: . * Page 90 of90 . *. . *-,, ,*-:*, 1.'1-"1 Final}}

ML16287A416

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ML16287A416

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