Text

Submittal of Discharge Monitoring Report for September 2016
	ML16287A416
Person / Time
Site:	Sequoyah
Issue date:	10/10/2016
From:	Pratt P; Tennessee Valley Authority
To:	Hall A; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Resources
References
	Download: ML16287A416 (97)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 October 10, 2016 Ms. Angela Hall Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11th Floor Nashville, Tennessee 37243

Dear Ms. Hall:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR September 2016 Enclosed is the September 2016 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period. The Toxicity Report for sampling conducted July 31 -August 5 is enclosed. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714.

I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.

reston P. Pratt (Acting) Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures):

Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved .

Name_~~-SEQUOYA~UCLEA~ LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01)

Add reSL _E. ~BO><.lQOO - - - - - - - - - - - -

_ _ _ _(lNTEROFFICE OPS-SN-SQM) _ _ _ _ _ _ _ _ TN0026450 101 G F - FINAL

_ _ _ ~ODDY *DAISY._I.N_l738L_ _ _ _ _ _ _ _ _ PERMIT NUMBER DIFFUSER DISCHARGE DISCHARGE NUMBER FacJilly_ ..JYA *.filOUOYAH NUCLEAR PLANT_ - - - - -

Locatio.!!_ _!:!AMIL TOJiCOUNTY.... _ _ _ _ _ _ _ _ _ _ EFFLUENT DAY

- - NO DISCHARGE D ...

ATIN:Millicent Garland 30 NOTE: Read instructions before completini:i this form .

PARAMETER NO. FREQUENCY SAMPLE

[:><:

QUANTITY OR LOADING QUALITY OR CONCENTRATION EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 30 / 30 RCORDR MEASUREMENT ** 37.4 04 0 CENTIGRADE 00010 1 0 PERMIT ******** ****

REQUIREMENT

- - - - ******** ******** Req. Mon. DEG. C. CONTI CALCTD EFFLUENT GROSS DAILY MAX NUOUS TEMPERATURE, WATER DEG.

CENTIGRADE SAMPLE MEASUREMENT

- - - - ******** .. ******** ******** 29 .4 04 0 30 / 30 MODELO 00010 z 0 PERMIT

- - - - ******** **1111r

- - - - ******** 30.5 DEG. C. CONTI CALCTD REQUIREMENT INSTREAM MONITORING DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. &

UPSTRM DEG.C SAMPLE MEASUREMENT

- - - - ******** .. ******** ******** 2.1 04 0 30 / 30 CALCTD 00016 1 s PERMIT "'******* ****"'*"'"' **** "'**""""""* ******** 3.0 DEG. C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS DAILYMX NUOUS FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT

/ g11 03 ******** ******** ******** .. 0 30 / 30 RCORDR 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ********

..... CONTI RCORDR REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** ******** 30 / 30 CALCTD MEASUREMENT 1807 03 03 0 TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. ******** MGD ******** ******** ******** MGD CONTI CALCTD REQUIREMENT EFFLUENT GROSS VALUE MO AVG NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT

- - - - ******** .. ******** 0.019 0.038 19 0 22 / 30 GRAB 50060 1 0 PERMIT ******** ******** **** ******** 0.1 0.1 MG/L FIVE PER CALCTD REQUIREMENT EFFLUENT GROSS VALUE MO AVG DAILY MAX WEEK TEMPERATURE - C, RATE OF SAMPLE ******** 0.1 ******** ******** 30/ 30 CALCTD MEASUREMENT 62 ** 0 CHANGE tut 82234 1 0 PERMIT ******** 2.0 DEG ******** ******** ******* CONTI CALCTD REQUIREMENT C/HR EFFLUENT GROSS QAILYMX NUOUS

'D-~-_;::

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Preston P. Pratt direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the 1/1 Cl(¥ TELEPHONE DATE

' person or persons who manage the system, or those persons directly responsible for gathering (Acting) Site Vice President the information, the information submitted is, to the best of my knowledge and belief, true, 423 84 3-7001 16 10 05 (Acting) Site Vi~e President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violalions. OFF~ERORAUTHORQEDAGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No closed mode operation . The following injections occurred: Flogard MS 6236 (max calc. was 0.03 mg/L , limit was 0.20 mg/L) , Spectrus BD 1500 (max calc. was 0.015 mg/L, limit was 2.0 mg/L).

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved .

Name _ _!VA-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR} OMB No. 2040-0004 (SUBR 01)

Add res.§_ __E.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _J!NTEROFFICE OPS-5N-S(llil _ _ _ _ _ _ _ _ I TN0026450 II 101 T I F - FINAL

_ _ _ ~ODDY - DAISY,_I.N_273BL _ _ _ _ _ _ _ _ .l ___P_E_R_M-IT_ N_U_M _B_E_R_ _I I DISCHARGE NUMBER I BIOMONITORING FOR OUTFALL 101 Fac.ill!Y_ JYA -_filQUOYAH NUCLEAR PLANT_ _ _ _ _ _

LocatiO.!L _!:!AMIL TOJi.COUNTY_ - - - - - - - - - - EFFLUENT ATIN:Millicent Garland I

From . YEAR 16 I *** NO DISCHARGE D ...

NOTE: Read instructions before completinll this form .

PARAMETER IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0

>< SAMPLE MEASUREMENT PERMIT QUANTITY OR LOADING 1-------------------+--------.--------.---

AVERAGE MAXIMUM UNITS MINIMUM Monitoring Not Required QUALITY OR CONCENTRATION AVERAGE MA

- X-IM

_ U_M_ __,__

U_

N_

23 IT-S~

PERCENT NO.

EX FREQUENCY OF ANALYSIS SEMI SAMPLE TYPE COMPOS REQUIREMENT 42.8 ********

EFFLUENT GROSS

.. MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** Monitoring ********

MEASUREMENT

- - - - 23 PIMEPHALES TRP6C 1 0 PERMIT REQUIREMENT

- - - - ******** .... Not Required 42.8 ""******* ******** PERCENT SEMI COMPOS EFFLUENT GROSS MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

~~

NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all allachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Preston P. Pratt personnel properly gather and evaluate the informalion submitled. Based on my inquiry of lhe person or persons who manage the syslem. or !hose persons directly responsible for gathering (Acting) Site Vice President 423 843-7001 16 10 05 the information. the information submilled is , to the best of my knowledge and belief. true.

(Acting) Site Vice President accurate. and complete. I am aware that there are significant penalties for submitting false I SIGNATURE OF PRINCIPAL EXECUTIVE

- - - - - - - - - - - - - - - - - J information, including the possibility of fine and imprisonment for knowing violations.

TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY OMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here}

oxicity was not sampled in September 2016.

I IPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDR ESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved.

Name _ _!VA~EQUOYA!:!_NUCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRI OMB No. 2040-0004 (SUBR 01)

Addres_L _E.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ .J.!.NTEROFFICE OPS-5 N-S<;llil _ _ _ _ _ _ _ _ TN0026450 103 G F - FINAL

_ _ _ _§_OD D Y - DA I S YJ~73BL _ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND FacJ.!i!Y_ JYA - SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _

EFFLUENT LocatiO.!L ....!:!AMIL TOJiCOUNTY_ - - - - - - - - - -

ATIN:M illicent Garland NO DISCHA RGE D ...

NOTE: Read instructions before comoletinQ this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAG E MAXIMUM UN ITS PH SAMP LE MEASUREMENT

- - - - ******** .. 7.7 ******** 8.5 12 0 5 130 GRAB 00400 1 0 PERMIT

- "'***** ******** ** 6.0 **1111utr*** 9.0 SU ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MINIMUM MAXIMUM WEEK SOLI DS, TOTAL SUS PEN DED SAMP LE MEASUREMENT

- - - - ******** .. ******** 5.9 5.9 19 0 1 / 30 GRAB 00530 1 0 PERMIT REQUIREMENT

.. ******** 30.0 100.0 MG/L ONCE/ GRAB EFFLUENT GROSS MO AVG DAILY MX MONTH OIL A ND GREASE SAMPLE ******** ******** .. ******** <5.0 <5.0 19 0 1/ 30 GRAB MEASUREMENT 00556 1 0 PERMIT ******#ntr ******** ******** 15.0 20.0 MG/L ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MO AVG DAILY MX MONTH FLOW , IN CONDUIT OR THRU SAMP LE 1.280 1.306 ******** ******** ******** 0 5 130 INSTAN MEASUREMENT 03 TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT Req. Mon . Req. Mon MGD ******** ******** ********

.. ONCE/ INSTAN EFFLUENT GROSS MO AVG DAILY MX WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPL E MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITL E PRINCI PAL EXEC UTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my II n ~

TELEPHONE DATE Preston P. Pratt (Acting) Site V ice President direction or supervision in accordance with a system designed lo assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false 1:1;!l:, Pra,;doot SIGNATURE OF PRINC IPAL EXECUTIVE 423 I

843-7001 16 10 05 TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AG ENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AN D EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATI ONAL POLLUTANT DI SC HARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved .

Na~ _ _!VA~EQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MON ITORING REPORT (DMR) OMS No. 2040-0004 (SUBR 01)

Add res_L _£'.. ~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _flNTEROFFICE OPS-5N-SQ.Nl _ _ _ _ _ _ _ _ TN0026450 110 G F - FINAL

_ _ _ __§_ODDY - DAISY._!_N_27384_ _ _ _ _ _ _ _ _ RECYCLED COOLING WATER PERMIT NUMBER DISCHARGE NUMBER Facjfily_ JYA -_filQUOYAH NUCLEAR PLANT_ - - - - -

Locatio.!!_ ..JjAMIL TOli_COUNTL _ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN :Millicent Garland From ~ 16 IYEAR I QAY 30 NO DISCHARGE Ixx I ***

NOTE : Read instructions before completinQ this form .

PARAMETER TEM P ERATURE, WATER DEG.

CENTIGRADE 00010 1 0

>< SAMPLE MEASUREMENT PERMIT AVERAGE QUANTITY OR LOADING MAXIMUM UNITS MINIMUM QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS 04 DEGC NO.

EX FREQUENCY OF ANALYSIS SAMPLE TYPE REQUIREMENT

- - - - **"***** ******** ******** REPORT CONTIN CALCTD EFFLUENT GROSS VALUE DAILY MX uous TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ********

MEASUREMENT ** 04 CENTIGRADE 00010 z 0 PERMIT REQUIREMENT

.. ******** ******llr* 30.5 DEGC CONTIN CALCTD uous INSTREAM MONITORING DAILY MX TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ********

UPSTRM DEG.C MEASUREMENT 04 00016 1 0 PERMIT

- - - - ******** ** ******** ******** 5 DEGC CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILY MX uous FLOW, IN CONDUIT OR THRU SAMPLE ********

MEASUREMENT

- - - - 03 ******** ******** **

TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT

- - - - Req. Mon. MGD ******** ******** ********

.. CONTIN RCORDR EFFLUENT GROSS VALUE DAILY MX uous CHLORINE, TOTAL RES IDUAL SAMPLE MEASUREMENT 19 50060 1 0 PERMIT REQUIREMENT

- - - - ******** .. ******** 0.1 0.1 MG/L Five per CALCTD EFFLUENT GROSS VALUE MO AVG DAILY MX Week TEMPERATURE- C, RATE OF SAMPLE ******** ******** ******** ********

CHANGE MEASUREMENT 04 82234 1 0 PERMIT ******** 2 DEGC ******** ******** ******** ** CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous SAMPLE MEASUREMENT PERMIT REQUIREMENT

(

1J~1:,

NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments ware prepared under my TELEPHONE DATE direction or supervision in accordance with a syslam designed to assure that qualified Preston P. Pratt personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering Pre*idoot 423 843-7001 16 10 05 the information, the information submilled is , to lhe best of my knowledge and belief, true, (Acting) S ite Vice President accurate, and complet e. I am aware that there are significant pen allies for submilling false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, incl uding the possibili ty of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved .

Name_~VA-SEQ U OYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01)

Addres_L _E Q._BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ -1J.NTEROFFICE OPS- 5N-SQ~)- _ _ _ _ _ _ _ TN0026450 110 T F - FINAL

---~ODDY - DAISU~736L _ _ _ _ _ _ _ _ RECYCLED COOLING WATER PERMIT NUMBER DISCHARGE NUMBER Fac.ill!Y_ JYA -_fil:QUOYAH NUCLEAR PLANT_ - - - - -

Locatio.D_ .J::!AMILTOJi.COUNTy_ _ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN :M illi cent Garland NO DISCHARGE Ixx I ***

NOTE : Read instructions before completinQ this form .

C><

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA SAMPLE MEASUREMENT

- - - - ******** .. ******** ******** 23 TRP3B 1 0 0 PERMIT *****lfr** ***"**** **** 42.8 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ********

MEASUREMENT ** 23 PIMEPHALES TRP6C 1 0 0 PERMIT

- - - - ******"'* 42.8 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

(\. fl

~LVJ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and alt attachments were prepared under my TELE PHONE DATE direction or supervision in accordance with a system designed to assure that qualified Preston P. Pratt personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering 1~cting ) Site Vice President the information, the information submitted is , to the best of my knowledge and belief, true, 423 843- 7001 16 10 05 (Act ing) Site V ice President accurate, and complete. I am aware Iha! there are significant penalties for submitting false I SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLAT IONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used 0'3nO 1 r./ 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved.

Name _ __!VA~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01 )

Add res,L ..f.. ~BOJ<.1900 _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _(LNTE ROFFICE OPS-5N-S(llil _ _ _ _ _ _ _ _ TN0026450 118 G F - FINAL

_ _ _ _§ODDY - DAISYJ.N_]7381_ _ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WAT ER FacJlj!y_ JYA -_fil:QUOYAH NUCLEAR PLANT_ _ _ _ _ _

LocatiO..!L _!:!AMILTOJi.COUNTY._ _ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN :M illicent Garland I

From . YEAR 16 I QAY 30

- - NO DISCHARGE Ixx I ***

NOTE: Read instructions before completinQ this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVE RAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) SAMPLE ******** ******** ******** ********

MEASUREMENT ** 19 00300 1 0 PERMIT

- - - - ******"'* **** 2 ******** ******** MG/L TWICE/ GRAB REQUIREMENT EFFLUENT GROSS MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** ********

MEASUREMENT ** 19 00530 1 0 PERMIT REQUIREMENT

.... ******** ******** 100 MG/L TWICE/ GRAB EFFLUENT GROSS DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE MEASUREMENT

- - - - ******** .. ******** ******** 25 00545 1 0 PERMIT REQUIREMENT

.... *******"' ******** 1 ML/L ONCE/ GRAB E FFLUENT GROSS DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMP LE ******** ******** ********

MEASUREMENT 03 TREATMENT PLANT 50050 1 0 PERMIT Req . Mon. Req. Mon. MGD ******** ******** ********

ONCE/ ESTIMA REQUIREMENT EFFLUENT GROSS MO AVG DAILY MX BATCH SAMP LE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREM.ENT PERMIT REQUIREMENT I '\r I'

V1~V~

NAME/TITLE PRINC IPAL EXECUTIVE OFFICER I Certify under penally of law thal this documenl and all auachmenls were prepared under my TELE PHONE DATE direclion or supervision in accordance with a syslem designed to assure that qualified Preston P . Pratt personnel properly gather and evaluale lhe information submitled. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for galhering Acting) S ite Vice Pres ident lhe informalion, the informalion submilled is , lo lhe besl of my knowledge and belief, lrue, 423 843-7001 16 10 05 (Acting) S ite Vice President accurale, and complete. I am aware lhal lhere are significant penallies for submilling false I SIGNATURE OF PRINCIPA L EXECUTIVE TYPED OR PRINTED information, including the possibilily of fine and imprisonmenl for knowing violations. OFFIC ER OR AUTHO RIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AN D EXPLANATION OF ANY VI OLATIONS (Reference all attachments here)

Du ri ng this reporting period , there has been no flow from the Dredge Pond other tha n that result ing from rai nfall. No D ischarge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used P::. n <> 1 r.f 1

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: August 26, 2016 I. Facility I Discharger: Sequoyah Nuclear Plant I TVA

2. County I State: Hamilton I Tennessee

3. NPDES Permit#: 1N0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1.579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. lQIO: 3.491

8. Outfall Tested: 101

9. Dates Sampled: July 31-August 05, 2016

10. Average Flow on Days Sampled (MGD): 1791, 1797, 1793

11. Pertinent Site Conditions: Production I operation data will be provided upon request.

12. Test Dates: August 02-09, 2016

13. Test Type: Sh011-tenn Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids ( Ceriodaphnia dubia)

15. Concentrations Tested(%):

Pimephales promelas: UV treated Outfall IOI: 10.7, 21.4, 42.8, 85.6, 100

UV treated Intake: I 00 Ceriodaphnia dubia: Non-treated Outfall IOI: 10.7, 21.4, 42.8, 85.6, 100 Non-treated Intake: I 00 16.PennitLimitEndpoint(%): Outfall IOI: IC22 =42.8%

17. Test Results: Outfall 101: Pimephales promelas: IC22 > 100%

Ceriodaphnia dubia: IC2s > 100%

Page 1 of90

18. Facility

Contact:

Millicent Garland Phone#: (423) 843-6714

19. Consulting I Testing Lab: Environmental Testing Solutions. Inc.

20. Lab

Contact:

Jim Sumner Phone#: (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone#: (256) 386-2787

22. Notes: Exposures to samples collected July 31-August 05, 2016 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to -intake samples resulted in no significant difference from the controls during this study period.

Page2of90

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 1O1. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY)

Sample Time (ET) Time (ET) Temp. TRC* Time (ET)

ID Collected Received (oC) (mg/L) LastUsedBv 07-31-16 0705 to t 08-02-16 0927 101 08-01-16 1307 1.2, 0.8' <0.10 08-01-16 0605 08-03-16 0857 07-31-16 0720 to 08-02-16 0927 Intake 08-01-16 1307 0.2 <0.10 08-0l-i6 0620 08-03-16 0857 08-02-16 0705 to 08-04-16 0906 101 08-03-16 1220 1.2, 1..

4t <0.10 08-03-16 0605 08-05-16 0907 08-02-16 0715 to 08-04-16 0906 Intake 08-03-16 1220 1.4 <0.10 08-03-16 0615 08-05-16 0907

\

08-06-16 0939 08-04-16 0710 to 101 08-05-16 1235 0.5, 0.7t <0.10 08-07-16 0935 08-05-16 0610 08-08-16 0853 08-04-16 0725 to 08-06-16 0939 Intake os~o5-16 1235 0.6 <0.10 08-07-16 0935 08-05-16 0625 08-08-16 0853

TRC = Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were wanned to test temperature (25.0 +/- l.0°C) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Page3 ot90

Pimephal'es promelas Ceriodaphnia ciubia Test Organisms:

1. Source: In-house Cultures In-house Cultures

2. Age: < 24-hours old < 24-hours old Test Method Summary:

1. Test Conditions: Static, Renewal . Static, Renewal

2. Test Duration: Until at least 60% of control females have 3 broods

3. Control I Dilution Water: Moderately Hard Svnthetic Moderately Hard Svnthetic

4. Number of Replicates: .1

5. Organisms per Replicate: 1O l

6. *Test Initiation: (Date/Time): 08-02-16 0855 ET 08-02-16 0927 ET

7. Test Termination: (Date/Time): 08-09-16 0848 ET 08-09-16 083 l ET

8. Test Temperature: Outfall 101: Mean= 24.7°C Mean= 24.8°C (24.2 - 25.1°C) (24.6 - 25.2°C)

9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville, CA).

Page4 of90

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration)

Conducted August 02 - 09. 2016 using effluent from Outfall IO 1.

Test Percent Surviving Solutions (time interval used - days)*

(%Effluent) 1 2 3 4 5 *6 7 Control, 100 100 100 100 100 100 100 UV-treated 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 98 98 98 98 100.0% 100 100 100 98 98 98 98 Intake 100 100 100 100 100 100 98 Control, 100* 100 100 100 100 100 100 Non-treated Mean Dry Weight (mg)

Test Solutions (replicate number)

(%-Effluent) 1 2 3 4 Mean Control, 0.565 0.482 0.548 0:478 0.518 UV-treated 10.7% 0.488 0.461 0.516 0.54~ 0.504 21.4% 0.527 0:465 0.430 0:449' 0.468 42.8% 0.481 0.509 0.537 0.528 0.514 85.6% 0.470 0.499 0.431 0.449 0.462 100.0% 0.466 0.502 0.534 0.501 0.501 Intake 0.479 0.511 0.495 0.460 0.486 Control, 0.587 0.555 0.525 0.423 0.523 Non-treated IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 42.8%

Permit Limit 23 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = 100/ IC2s Page5of90

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration)

Conducted August 02 - 09, 2016 using effluent from Outfall 101.

Percent Surviving Test (time interval used - days)

Solutions 1 2 3 4 5 6 7

(% Effluent)

Control 100 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/7 days)

Test Solutions Data (replicate number)

(% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 31 31 31 34 32 30 31 28 29 29 30.6 10.7% 31 32 32 35 30 33 35 29 33 32 32.2 21.4% 31 32 32 34 32 36 30 31 34 31 32.3 42.8% 34 35 35 31 33 34 32 32

~

37 30 33.3 85.6% 32 32 34 36 34 36 30 34 30 33 33.1 100.0% 37 35 36 31 36 35 38 37 33 36 35.4 IC2s Value: > 100% Calculated TU Estimates: < LO TUc*

Permit Limit: 42.8%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA ,

Lower Limit: NA

TUa = 100/LC5o: TUc = IOO/IC2s

/

Page 6 of90

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration)

Conducted August 02-09, 2016 using water from Intake Percent Surviving Test (time interval used - days) .

Solutions 1 2 3 4 5 6 7

(% Effluent)

Control 100 100 100 100 100 . 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7, days)

Test Solutions Data (replicate number) ;

(% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 33 31 29 31 28 32 31 31 30 30.5 Intake 35 33 35 32 37 38 32 36 33 34 34.5 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = lOO/IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results OC2s)

Pimephales promelas August 02-09, 2016 0830 7-da~s KCl 0.79 g/L Ceriodaphnia dubia August 02-09, 2016 0915 7-days NaCl 1.07 g/L Page 7 of90

PHVSICAUCHEMICAL SUM M.ARY Water Chemistry Mean Values and Ranges for UV-treated Pimepha/espromelas and Non-treated Ceriodaphnia dubia, Sequoyah Nuclear Phmt (SQN), Efiluent Outfall I OJ and Jntake perform::d Augtmt 02-09, 2016.

Test Sample ID Tempemture (0 C) Dissolved Oxv11:en (m!!:IL) pH (S.U.) Conductance Alkalinity Hardness *Total Residual Initial Final Initial Final Initial Final (µmhos/cm) (mg/L CaC03) (mg/L CaC03) Chlorine (mg/L)

Control, 24.7 24.5 7.8 7.6 7.89 7.81 319 60 91 -

NurHrl?'utcd 24.6 - 24.8 24.3 - 25.0 7.7 - 7.8 6.4 - 7.9 7.73 - 8.09 7.51 - 8.06 308 - 330 59 - 62 88 - 96 - -

Control. 24.7 24.5 7.8 1.4 7.93 7.78 312 59 89 -

UV-treated 24.6 - 24.8 24.4 - 24.7 7.8 - 8.0 5.5 - 7.9 7.82 - 8.02 7.48 - 7.89 301 - 322 58 - 59 88 - 90 - -

I0.7%

24.8 24.5 7.8 7.4 7.94 7.75 304 - - -

i co

~

21.4%

24.7 - 24.9 24.2 - 24.7 7.8 - 7.9 _5.5 - 7.9 24.8 24.5 7.8 1.4 7.83 - 8.04 7.49 - 7.86 7.95 7.76 294 - 311 293 24.7 - 25.0 24.2 - 24.7 7.8 - 8.0 5.4 - 7.9 7.84 - 8.04 7.50 - 7.85 285 - 300 - - - - - -

~

.i: 42.8%

24.8 24.5. 8.0 7.4 1.95 7.76 271 - - -

~ 24.7 - 25.0 24.3 - 24.7 7.9 - 8.2 5.5 - 7.9 7.85 - 8.03 7.51 - 7.86 265 - 277 - - - - - -

~ 85.6%

24.8 24.5 8.0 7.5 7.97 7.77 226 - - -

24.7 - 25.0 24.4 -. 24.7 7.9 - 8.2 5.5 - 8.0 7.86 - 8.04 7.52 - 7.89 223 - 233 - - - - - -

24.9 24.4 8.0 7.5 7.99 7.77 209 76 83 <0.10 100%

24.8 - 25.l 24.2 - 24.7 7.9 - 8.2 5.5 - 8.0 7.88 - 8.08 7.54 - 7.89 205 - 215 74 - 78 82 - 86 <O.IO - <0.10

<0,10 24.8 24.5 8.0 7.6 7.99 7.81 205 73 78 Intake 24.7 - 25.0 24.4 - 24.6 8.0 - 8.2 6.3 - 8.0 7.89 - 8.07 7.65 - 7.91 199 - 209 72 - 74 73 - 82 <0.10 - <0.10 Control, 24.7 24.9 7.8 7.9 7.89 7.94 319 60 91 -

Non-treated 24.7 - 24.8 24.6 - 25.0 7.7 - 7.8 7.8 - 8.0 7.73 - 8.09 7.86 - 8.03 308 - 330 59 - 62 88 - 96 - -

10.7%.

24.8 24.8 7.9 7.9 8.01 7.92 305 - - -

24.7 - 24.9 24.6 - 25.0 7.8 - 7.9 7.8 - 8.0 7.91 - 8.12 7.85 - 8.02 295 - 313 - - - - - -

21.4%

24.8 24.8 7.9 7.9 7.99 7.92 294 - - -

~ 24.7 - 24.9 24.6 - 24.9 7.8 - 8.0 7.8 - 8.0 7.90 - 8.10 7.85 - 8.01 283 - 304 - - - - - -

.s:: 24.8 24.8 7.9 7.9 7.99 7.93 270 - - -

..:: 42.8%

24.7 - 24.9 24.6 - 25.1 7.8 - 8.0 7.8 - 8.0 7.90 - 8.09 7.86 - 8.03 257 - 277 - - - - - -

~ 24.8 24.8 7.9 7.9 7.99 7.95 225 - - -

-~ 85.6%

~ 24.7 - 24.9 24.7 - 24.9 7.8 - 8.0 7.8 - 8.0 7.91 - 8.09 7.89 - 8.04 216 - 235 - - - - - -

24.9 24.9 8.0 8.0 8.01 7.96 205 78 83 <O.lO 100%

24.8 - 25.0 24.7 - 25.2 7.8 - 8.1 7.9 - 8.J 7.92 - 8.11 7.90 - 8.05 197 - 215 76 - 80 82 - 86 <().10 - <0.10 24.9 24.9 8.1 8.0 8.00 7.98 205 73 78 <0.10 Intake 24.8 - 25.0 24.6 - 25.2 8.0 - 8.2 7.9 - 8.2 7.93 - 8.10 7.92 - 8.08 197 - 211 72 - 74 73 - 82 <0.10 - <0.10

Note: Total residual chlorine was performed on non-treated Outfall IO I and Intake samples.

Overall temperature (0 C) Average Minimum Maximum Pimephales prome/as 24.7 24.2 25.1 Ceriodaphnia dubia 24.8 24.6 25.2

SUMMARY

I CONCLUSIONS Exposures to samples collected. July 31 - August 05, 2016 from Outfall 10 I resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > I 00 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Page9of90

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EP A-82l-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas Samples used in the fatl}ead minnow test were exposed to UV light for two minutes prior to introduction of test organisms. UV treatment is used to control interference of fish pathogens. This treatm~nt method was approved on November 23, 2015 by the State of Tennessee in a letter frdm Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring. *

2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

{

2. Ceriodaphnia dubia None Page 10 of90

' PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance.lOgbooks.

3. Temperature was measured by SM 2550 B-2000.

4. Dissolved oxygen was measured by SM 4500-0 G-2001.

5. The pH was measured by SM 4500-H+ B-2000.

6. Conductance was measured by SM 2510 B-1997.

7. Alkalinity was measured by SM 2320 B-1997.

8. Total hardness was measured by SM 2340 C-1997.

9. Total residual chlorine was measured by ORION 97-70-1977.

)

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013 .. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl.

2. Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 11 of90

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 12of90

Sequoyah Nuclear Plant Biomonitoring August 02 - 09, 2016 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 13 of90

Table B-1 . Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016 Date Sodilim Towerbrom PCL-222 PCL-401 Cuprostat-PF H-130M Nalco lH50M'"

Hypocblorite mg/L mg/L mg/L mg/L m,g!L , 73551 mg!L TRC Phosphate Copolymer Azole .Quat mg/L TRC EOIPO 02/06/2005 <0.0042 ' 0.028 0.010 02/07/2005 <0.0116 0.028 0.010 02/08/2005 <0.0080 0.028 0.010 02/09/2005 O.Q199 ().028 0.010 02/ 10/2005 <0.0042 0.028 0.010 0211112005 0.0155 0.028 0.010 0610512005 0.0063 06/06/2005 0.0043 06/07/2005 0.01 03 06/08/2005 0.0295 0610912005 0.0129 06/l 0/2005 . 0.0184 07/17/2005 0.0109 0.026 0.009 07/ 18/2005 0.0150 0.026 0.009 07/19/2005 0.0163 0.026 0.009 07/20/2005 0.0209 0.026 0.009 07/2112005 0.0242 0Jl26 0.009 07/22/2005 0.0238 . , Q.054

O.Q18 10/30/2005 0.0068 10/3 1/2005 0.0112 11101/2005 0.0104 11/02/2005 0.0104 l l/03/2005 0.0117 11/04/2005 0.0165 l l /14/2005 0.0274 11 / 15/2005 0.0256 l 1116/2005 0.0234 11 / 17/2005 0.0231 11118/2005 0.0200 l lil 9/2005 0.0116 Page 14 of90

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016 Date PCL-401 Cuprostat-PF Nalco H-150M MSW mg/L mg/L 73551 *~ 101 Copolymer Azole mg/L quat mgJL EOIPO Phos hat e 11112/2006 11/13/2006 *0.037 11114/2006 0.037 11/15/2006 0.037 11116/2006 0.037 11/17/2006 11126/2006 11/27/2006 11/28/2006 11129/2006 11/30/2006 12/01/2006 05/28/07 0.015 05/29/07 0.036 0.015 05/30/07 0.017 0.036 0.015 05/31/07 0.036 0.015 06/01107 0.017 0.036 0.015 06/02/07 0.015 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/ 13/08 04/14/08 04115/08 04/16/08 04/ 17/08 04/18/08 10/26/08 10/27/08 0.017 10/28/08 0.041 .

10/29/08 O.Dl8 0.041 0.030 10/30/08

0,041 0.030 10/31 /08 0.041 0.030 Page 15 of90

I I

Table B-1 . Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016 Date 'Sodium Towerbron Cuprostat H-l~OM H-150M Hypochlorite mg/L -PFmg/L mWt mg/L mg/L TRC Azole QUat Quat

'TRC 02/08/09 0.0197 01109109 0.023 7 0211 0109 0.0104 02111109 0.0155 02/ 12/09 0.0106 02/ 13/09 05/ 10/09 0.0129 0511 1/09 0.0415 0.0446 05/ 12/09 0.0053 0.0396 0511 3109 0.0049 0.0396 05/ 14/09 <0 .0141 0.0397 0511 5109 <0 .0160 11 / 15/09 0.025 11 / 16/09 0 .0152 11117/09 0.0255 11 /1 8/09 0.0306 11119/09 0.0204 11 /20/09 0.0093 05/09/1 0 0.0192 05/ 10/1 0 0.0055 05/ 11110 0.0100 0511 211 0 0.01 7 1 0511 3/1 0 0.0041 05il 4/IO 0.0099 Page 16of90

Table B-1 . Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016 Date PCL-401 CL-363 Nalco SpecttuS. H-150M . MSW Flo guard mg/L mg/L 73551 CT1300 mg/L d 01 MS623 6 Copoly- DMAD . mg/L mgJf ' Quat mg/L mg/1.

mer EOIPO Quat . Phos- Phosphate hate 10/31 / 10 .-

11101/10 11 /02/ 10 ~

11103/10 11 /04110 11105110 05/0l /20ll 05/02/2011 0.04 05/03/2011 0.04 .

05/04/2011 0 .04 05/05/2011 r 0.04 0510612011 *. '. -

11106/2011 ll /07/20ll 11/08/2011 11 /09/20 LL LL/10/2011 "'*

11 /1 1/201 L 05/06/2012 05/07/2012 05/08/2012 05/09/2012 05/10/2012 05/11 /2012 08/ 12/2012 .

0.029 08/ 13/2012 *,

0.029 08/14/2012 0.02 9 08/15/2012 0.029 08116/2012 0.029 08/17/2012 0.032 05/12/2013 05/ 13/2013 0.064 05/14/2013 0.064 05/15/2013 0.064 05/16/2013 0.032 05/17/2013 o.o:u 09/15/2013 '*"' **. ~

0.03 09116/2013 *.

09/17/2013 ~.

0.03 0 .036 0.03 09/18/2013 0.036 09/19/2013 . 0.03 O.Q3 09/20/2013 * ,; -

0.03 Page 17 of90

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016 Date Flo guard MS6236 mg/L

'ii* Phosphate 05/04/2014 0.0118 05/05/2014 ,: 0.0112 05/06/2014 0.0096 05/07/2014 0.0164 05/08/2014 . 0.0235 05/09/2014 0.0110 09/07/2014 .

09/08/2014 09/09/2014 09/10/2014 09111 /2014 09/ 12/2014 08/09/2015 08/10/2015 08/11 /2015 08/ 12/2015 0.03 08/ J 3/2015 0.03 08/14/2015 0.03 10/18/2015 10/19/2015 10/20/2015 l 0/21 /2015 10/22/2015 l 0/23/2015 .

05/ 15/20 16 05/16/2016 '

05/17/2016 05/ 18/2016 .

05119/2016 05/20/2016 08/01/2016 08/02/2016 08/03/2016 08/04/2016 08/05/2016 Page 18 of90

Sequoyah Nuclear Plant Biomonitoring August 02 - 09, 2016 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 19 of90

BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of _I_

Environmental Testing Solution, Inc. Delivered By (Circle One):

Pect Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P~. Number: N/A Asheville, NC Other (specify):-::OY\iC..\*el ~uer F~lity Sampled: Sequoyah NP 28801 NPDES Number: TN-0026450 Phone: 828-350-9364 Fax: 828-350-9368 Flow Rain Event?

Collection Date/Time (MOD) Laboratory Use (Mark as Appropriate)

SllUt ~d Yes tfYcs, No Trace ETSLog Arrival Temp. By Time

Appear*

Inch ca Number "C ~ &!ICC 11*1,, /{;

SQN-101-TOX Comp 2(2.Sgal) o'loS:.. i:::>~o5£t" l'1<ff>,gJG, \ .\3

'?.J/-/{., 1 - 1~1 Comp 01to<U' /JfP. 20e 1(2.5 gal) x Sample Custody- Fm in From Top Down Date/Time Reee.ived By (Signature):

- ~

Tn$tru(:tion$: Clients Should tlll in all areas except those In the "Labora10ry Use" block. Bi<lt11oilitoring samples arc preserved by storing them between 0°C and 6°C and shipping them in ice (samples should never be frpzcn), ihehold time for each $J1llplc is 36 ~ours from the time of eollection. Therefore; please collect and sbip in such a wa~ that the laborlitory will receive.the samples with amjjle time to inltiat'e testing Within that time frame. Samples shipped overnight on Friday via FedEx or UPs must be marked for Saturday delivery or they will not mive until the followirJg Monday.

r Whole Effluent Sample Receipt Log

sample temperetllre performed 111l11C S.mplt Rece.vln1Tn11mometer. $N llOSBOQll~

Date Time Received Received *sample

Project Sample s.ample name and description State C:ommenu Received ~etelved by front temp. 1°c) hUli'll;ler numbet. -

011;01-lfi 1013 K. Keenan J. Kennedy ' 4.0 116~6, l6Q801 .0I Tu,cl<<tsel1ee WWTP ...

NC *-

08*0H6. 1307 J. Sumner TVA Courier 1.2/0.8 . 1163'7 1:60801 .02 TVA - Sequoyah NP - 101 TN 08~01-16 1307 J. Sumner TVA Courier 0.2 U6~1 l~B<Jl .03 . 'TVA.* $eauovah NP - Intake - TN SOP G4 - Exhibit G4.2, revision 01-03-12

Page BIOMONITORJNG CHAIN OF CUSTODY RECORD of aent: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

FedEx UPS Bus Client

!i>ject Name: Sequoyah NP Toxicity 351 Depot Street.

~*Number: N/A Asheville, NC Other (specify): :Son \<!. JJe \i \J~ t

~ility Sampled: Sequoyah NP 28801 NPDES Number: TN0026450 Phone: 828-350-9364

. Ma.rcc,;.5 O -.>*?ccrc.. r Fax: 828-350-9368 CoII ectedB y:

Container f'1b1~

~ I \.\o!l- Laboratory Use Number & Flow Rain Event?

Collection* Date/Time Volume (MGD)

(Mark as Appropriate)

~1--~~~ .....~--'-~~-+/-:: Collected lfY~,

Trace ETS g ArrivalTellljl * . By Tlm*e Appear*

S1a:11 End Yes No Number *c lnch~s

~' BQCC

'b*Z>l~ '8 -J - }~ \.'2. \A *c...

SQN-101-TOX Comp f)PJD5'<tt.~ D&D5r.; x* o .o3 l~f18ba. 2:3 ~ h.1..() i 8 --z~ /& , ~ ~ 3 *16 Comp 1(2.S gal) \. '-l 'L 0? J$" "£1: 0 Io e.T. x Sample Custody"- Fill ln From Top Down Relinquished By (Signature}: Date/Time Received By (Signature):

So.""\C..

l:.O.\.<<.tj

\"1..'1. o er Instructions; Clients should fill fn all areau.xcept those in the **Laboratory Use" block. Biomonitoring samples 11rc preserved by sto-ring*thern at 6°C and shipping them in ice. Thehold time fQr each sample

is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefote. please collect and ship in such a way that the labpratory will receive the samples with ample time to Initiate testing within th~t time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked fot Saturday delivery or ihey will not arrive until the following Monday.

Whole. Effluent sample. Receipt Log Date Time' ~ecelv1,1d fl~cf!IVed. * *$llmpia 'Project sam~le

stat~ '

Sample name.a11d descriptfi)it *Commehts Received .. . R11~el!ted. by .ftdrn: .. remp;(~Cl . numbe~ i:iumb!ae' os~os~:i:G. '0940, K>Keenan. ues: ... 2~6.' (" 11650 . aooaps,,01

RockJnehamWWTP * ',N<!

os~os*16. 0940 . K Keenan. ups; 1.l1; 11651 160803' .02 . SoLithiCarv WWTP NC 08*03"3;6: . . 05140: l<iKe1man .UPS. " ** 1;3' 11652* . 160803.*03.. MoceheadCltv WWTP** <NC

... 08~()3~16' :'1021' K~ K~enail'. Fed-EK . ., 2.2 . 11653 . ~ 160803: ,04 BeleWs.CrilekSS, .,,., : NC * ..

.OB-03~6 1021 .K. Keenan . ,Fed.- Eic 1;5 11654 160803.0f BrunswlckCountvWTP *. NC<

os;,oHs* . J,02:1.

K.Keenan Fed*.El<.. 1.0.: 1* 11655' 1608l>3 *06 Oi:>bsonWTP NC.

08-03~16 1021 {(;Keenan. Fed.-.Ei< *2~4** 11656.: : 1.60803, ;07 ' MarshallSS ... NC.

0S;.03,1s 1021:. .* K. Keenan** Fed* Ell 2;1 UGS.7 . 160801r.08< NolthCarv:wwrp** NC' 08'03-16'. 1021. rt Keenan* Feit.Ex** !;:J! 116Str . 160800.*09* Jameslougtilrit WWTP NC OS*OlHG 1021; .K.,Keenan .. Fed,-£x, l.O 11659 ... 16080L l 0> Performance fibers NC:.

. 0&-03*1.6 . .

1021 .K. Kee(ian* 'Fed*E'f< ..(J'.!1: .11660 .**.1sosoa:,.n*** Releillh*CC. NC:

.oa-oa;.16, 1021 K;Keenan. Eed*Ex* to .'11651, . 1S0803 ,ti soarks RoadWTP ** N.C 08*03~16' 1021 !(;:Keenan, ;fed,.,£x; 1:() 11662 .. 160803 ;J3: .; lone CreekWWTP NC

' 08~03~16 1021.* K.ilteenan* Fed'-Eic l;S 11663' 160803 .14* McGUlre NS .iQOf' NC

.08*03-16 1021 K.Keenan 'Fed*EX 1.0. 11664 . . 160803; JS :*. McGuire.NS;. 002 NC os*'O.!H& .1021 K.'Keenan* Flid*E1c i.o 116~ 160803 .16 ManteoWWTP NC 08'*03"16 1021- . It Keenan Fed-Ex** ***1A 11666 .. ' 160803".1'7 Mavo steam Electric Plant NC OS.03~16 ... 1021 !(;Keenan Fed-_Ex* 2.6: 11667 160803 .* 18 Wai!'hln:liton.WwrP* NC O~OlJ.;16* *1021 IC.Ke~nan .. Fl!d.::;*E)<* ..13 11668 . '160803 .19 Woodliik'e. Yacht Club* *.:NC.

0~03~16 .1021 l(,l(eenan Fed~*El< 2.1 '11669 1&osoa* .ao PCS*Phosphate; Inc. NC*

08*03~16 .1021 K. Keenim Fed~ flt.

,n 11670 '.1'60803' ,21 Carolina Beach WWTP NC QS-03*16 *1oss K.Keenan* J.Kennedv 4.0; 11636 l608Q3 .~2. Tuckaselgee WWTP NC 08*03-16 .1220 J;sumner .TVA*Courler "1:2/1.4 11631 160803* ;23 TVA ~*seauovah. NP

101 TN 08*03;16 1220 J*. sumner .. TVA courler

1.4 .11'637' .160803* ,24 JVA;-Sequovah.NP' ~*intake

TN 08*0~16 ** 1zss . K. Ke-enan' Dash Courier 0'.3 . 11671 160803 .25: OWASA NC

O&!l3-16 1500' J:Sumner TVA Courter .O.S': .. 11672: 160803 ;26 TVA* Shawnee fp.,. OOlAM KV-08*03-16* 1500 J.Sumner TVA Courter o;9' . . 11672 1'60803 ,27** TVA~ Shawnee FP'* fntake AM KY*

08*03*16 1500 .J,Sumner TVA Courie( ;*o.a: . 11672: 160803 ;28 TVA' Sha\Nnee FP'. 001 PM KV*

.. 08-03-16 .... 1500.* J. Sumner *.... ,,. TV.A Cci.urler

1.3 11672 160803' .29.. TVA .. Shawnee rP ~:intake.PM ~ .,,, KY SOP G4 *Exhibit G4.2, revlsion 01-03-12

BIOMONITORING CHAIN OF CUSTODV llECORD Page_l_ of _1_

.. 1Client: TV A Delivered By (Circle One):

EnvironmentaJ Testing Solution, Inc.

~ Project Name: Sequoyah NP Toxicity

. Asheville FedEx Location. FedEx UPS Hus Client

~

c P.O. Number: NIA 628 Patton Ave. Other (specify): Sol!Ji~l.*vuy

~ Facility Sampled: Sequoyah NP AsheviUe, NC 28806 General Comments: {fj @ z . . r VIi*

!Oo~ ~?/~ i~Jt>lti'~eri 071 c NPDES Number: TN0026450 Phone: 828-350-9364 ht;i:.5(A?1/t'.$ ro~ ~ ~ ~µ.,.,-e ()itk lei

~-'!h'l t v Fax:

~~l'~~~/j~~~

828-350-9368

'./- -* **: __,._ ,,. \ £' ~*'

~~ .,,.. A 0 *

~bt~.5 Midt..Jet'( ttill~Je~-t J:eJ)I p>-r_J,bt:_

v v ./ '* F Field ldcnlilJcation I I Conu1incr Flow P/U)l~ I '~1-GrabiCornp. 'Number& Rain fiv.cnt?

Sompic Description Colieciion Dateffimc Volume (MQD)

(Mork as Apprnprfaic) l.aboratary Use Cull1:c11:d -

~-

Stnn "F.m.1

$-5', I<.,

-** -- Yes rrv,s, Inches No Trace F.TS log Nutnbcr Aftivol Temp.

("Cl

'Ry

.a-Time- Appear*

once SQN*IOl*'rOX Comp

<l~'l*ll.P

()'110~ ~- O(..,.lD~'f 2(2.5gal) !*193.o~S x o.L\'t .

lbitJoSIS o.s o:'\'C.

I a' \~'; -;.

~-1.Ht., <6-5~ It.:. .,_

SQN*llff*TOX co,np D1Z.5e . DIPZ..

5 I 1(2,5 gaJ) ff}J!I -x *o.t.ft '"'~'"

().~ t.

~

\1.)S Sample Custody- Fill In ftom Top Down ~ UJs"U>>l>"'I StA\S llilT'f!(.M. SN-\J\e$

U,U\ )6) .~ &Cltlb \Q~Drt'l()N. \~ _

Re~quished Sy (Signature): Da(e/Timc Received by (Signature):

Oate/Ttme ~ 1to) 1\\,.1

,_ '.wa: _-. *-"

-~~r ./

/

-~~ . .

Y*Gik/t>3 C.OE ~ /J~ ( -, ~9'A~.Of'-o;:./b ()~~"J, () ~

-~ ... p*~"!"' U' f:," ~* t

~I(.

~If 9--1 ii.. M**~ CJ y-..p$'-l&.J/J.~ ... ~~I* £~ O(*~*llo t"L~S tr r '-J ..

lnstructie>ns: Clicms should 1111 in illl arcas except those in the "l.obnratoey Use" bfilck. Binnionitnring sumplcs ore preserved by storing them between 0°C and 6°C and shipping lb.em in ire (snmplcs sJ1ould nllver be fro7.eo). The iiold time ror cuch sample Is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from theJhne of collection. Thercforii, please collect nod ship in such IJ WU)* that the faboratory will receive lhe s:implos with umple lime lo initiutc !csllng within thnt tirnc frame. Sample:t shi11i1cd 1wcmight on Friday via FedR" or UPS must Ile marked for Sowrday delivery or Ibey will not. orrl\'e until the lbllowlng Monday.

('-.

Whore Effluent Sarnple Receir;t Log

~ Date Time Received Re~el\l'ed *sampi~* PfoJect Sample CQ Sarnple name and description

C'D !Jecelved. Received bV. ftom . T~mp. (~O . *numlli!t* nlimbet l'I.,) *Oa~05~15 \JPS.. 0.6 . ..11650 . . : :.160805 ;OJ: Ror::khighamWWJP: NC-01

:0946 Kd<'eeoan

08*0~16 0946 K.K~enan 0 .. ,

..... *os~os~1F ..... ::.1011. -*-

K.: Keeiien* *. Fed~ ex.

J:a 11653 . <160B'OS ,.;03 . Belews Creek ss

Ne (0 os~os~1is K.Keenrin**

l011 ..

Q

. OS*O!i*l6 1011 K.Ktlenari

Fed ~ Ex 1.1 11655 160805:.05 D6bsonWTP NC 08-.05-16. 1011 .K;Keenail Fed* !iii *** a.s 11656 *1aosos .~06' Marshiil!Ss

Ne.

08-05*16 101r* : t<l Keenan.

Fed ,,ti(. .o.s 11657_ *. *160805 ,07 North Cary WWTP NC 08*05~16' 1011 . K* Keenini . F.ed. E'X o.a 11658 . - J60BO!F.()&" JarnesfoughllnWWTP . NC

~

1011 K.Keel'ian Fed - t:lf* 1.1 11659 1608US' .09 Performance Fibers *

NC os~os~1s**

1011 1011 .*~.

os,os-1.s._ 1013,:. K;Keellan 08*05*16: 1011 It Keenan 08~05~16 1203 K.K~hah H* Bingham 1.3.' 11636;

160805 J4

Tuckaseli;iee WWTP' NC 08-0S~lEi 1235 * .... J.,Sumner: TVA Coufler O,S/0.7 . 11637 160805'. .15: TVA - Seauo\.iah NP ~101 . ' TN

'08-05-16 1235 J. Sumner* TVA Coorier D.6 11631 160805 , 16 1VA .~ Seq~ovah NP* Intake TN 08-05-16* 1301

J. Sumlier Dash toifrler , 130M

i: 11s1:r 160805 .1 T *. OWPSA ~ Mason i:am1s . NC SOP G4 - Exhibit G4.2, revision 01-03-12

~~ere

~'c~ q *.ik.:.-,

j,__,~...._ ....

P~ge. l Qf'l I.

Chronic Whole Effluent'Tr,xiciq T~t (,E!P;.;\;~21..~':-D2~ot31\fetmjd :to,(l.U}

Species:. PiHtep[zales pro111e~a,s

Client: Tennessee. Vallev Auth&r.ity Cou)1ty; HamiltOJi Facility; Sequoyah Nuclear Plant* Outfall: 101,

NPDES #: TN002645(l . . ..

Proj~t #: \\I.~"'.\

Dilution orevarn.tion i1tfQr11.iatf(Jn: ..

- - .toinnleitis:

Dilution prep (%,) 10:1 21.4 42:$ 8$:6, .roo .Each oon~~ttaifun;*Wa$ JJV-ttea~

Effluent volume (ml.}. 'i67~}. 535 1()70 '2140 2$00 for: 2 minute$ t:0:remove :"Patho.e:enie Diluen1.voh,m1e.(1nL) *~2.$* '19~5 1430. 360. 0, r.ltterl'erences..

Total *volume {ml:.) 2SOO :uoo** is(}()*' .. 2500 2500 Test orea11lsnt}~fonnation:. .."f/tt$i inl~'f'J1~ati:01t:

Organism source:

In-hctuse culture *Ratidorilizing teml:>late: , 'Tc-fIJU(Q.. .

Age: < 24-bours old ,1ti~uba1QrI~u~ei~~a I '""\ t.

-* .shelt'b>ta~lOJl; Snawndate: .* CJ'\~ t.2'- \\:, . Ailei11ia,C1tM:number. l CHM882*

Hatch dates and tim~: Ot.*o\~\t.. l<Stit>- -re *})IJifngin/ornltlffemfor 1v~fji#t o(~GL.;\\Q. .&~ .detenN.11tlit1on: . *

Transfer vessei infonnation; * .pH?O t*.t>lo: s.th :Date /Time m/oven:

T¢inperai;ute;? '\Sc* \ oc; ,

lnitiafiov¢J!.tim'1Pera.l::ilm:: '

Average transftir v{)lUme: *,OjhnL ~~ d\a>

\o.ort

'"1."\.~'1~

Daily feeding a11d renttw<<l info.rmation:.

Dny D.ate M-Ornin~ *fee.Gin~ Afterno~ feedi*g Te~ Jniti:ition1 rencw:il,,,.......

pr* .

teniJinati'o.n

__ S:imp~e nuirillers ~d *. Masw bat:ch wi~

-Time Tlin~ I .A1u1l~t Qntfa11'.l0l . I h!ta.ke 0 08-02-16 .1~.4~\;~ . :1¢i~~~1t.:. .

Os.-0.3-16 \.1o ottlt-D?> *. Hn~-a.tlo.

2 08*04-16 '"-'ui(i'b.~: . loa.m..t~~

3 08-05~16

- itq".11.fm.~'2i! lt1t'*Aa*11, A 4 08*06-\6 . .,ok*fn.-c1c,A 5 08-07-16 bk4n.*L(Dl 6 08-08-16 ,. t*fn.*l~

'l' ".~-,. ;/"" * .....

7 08-0~-16 **, ,,~. _:i;,;.:,::_.,,,., ...<<

Co111ro/.i11formatioit.: U\Jf

% Mortalitv: 7.,;0ay:icso Averag.e weight per itiitial.iarvae:: 1:1 NOEc*.

Average weight per si.irViving l?.,rvae: :~o.2S!ll.f!llarva¢ 1*t.O"Et'::

'riv

,.l~

Page 26 of90 .SQP*AT:20*"-Exbi~t,AT.2.,0:3i.tevi~ion LJ-OJ.,14

Page2of7

Sp~ies: Pimepllnlespro~ins .

C.Uent: TVAI SOOuoyah .Nuclear..Piant, Outfall 101;*nv-~tet1:* l>ate: 08-02-16 StU'J!iilitl trnd Gtowtlz Data CQNTROL. 10.7% 21.4%

,;/\: ... 1\ c I) E F G. H l. .J K L w 10 10 w to 10 ,,,,

10 10 IO 10 10 IO ft): (0 {(j 10 to l*D lO to {Q {0 10 /{)

2:

lb /f) to ro LP !,.,

CQ' lo.. <o f O 10 !{) 10

tc, llJ lb 10* 'l() ~b J.() JD 10 10 10 It)

]Q *lo Jb ro la 16 lb ttl IC /() /0 10

' JO*..... JO JO .t D . lo*

Hl

f0 .::.

hJ ID fu 10 10 to l*D ro lO* IO Jt>. 10 l:b lO lD ro IQ IQ ro re ID . to . IC lb ID JQ /0 /Q I'()

C =Larvae weight (ing}.::i B-A.

Hand calculated" : 5,.;.'S. c{,tz.. S..tlt '{:ti' Cl.it q,.~\ ~.J~ S.c.tC\ S. '.'2:l ~. ~S c.t.~ "i.'-t'\

wr,yst: ......_____....,1\" *~*" '"*.- - . - -

1 ... _ .......

. ~A'I!

' (?J;.,'7~.:.

Co'mment tC>i:fd: c =crew-. d d,ead,. rg*7 :fi!ngu~ k kiU~ m'."". miSslng; .sk ;= si~k, Sill =*unusually 5mall, lg=> uruiSUalJY large, d&r = decat)ted and retumed,,w= wounded, . ., .

Page 27 of90 *SOPAT20-ExhibitAT20.3,revision 11-01-14

Species: Pimeohales pronz:e/as _ _

Client: TVA I Seguovah Nuclear Plant_ OutfaU101. UV~trefitedc S111~vlvalmn'f Gi'owl/J. Datil-8S.1io/o iJOO%

p _(~ --

a *s T ,a*- V: \\f< X-lQ 10 10 to lO 10 10 [{) IO 10 to 10 l

to tef 10 10*

.;. ,, ro lCl lO ro ID 2

i~ (:"

~ - lD . l.0 _ f"_ y lO:*: ( t:) fQ*.

3 4

t0 ---10 lO '10, 6

\('.) "\ lO. (i) 0) .. ~

7

/O : I 0 * -l Q (0 IC - Hl LQ. *Pt

. 10* .1:0 .. IO 't A= Pan weight(mgL ._. i_{' _ . ..

llf* . . - :* *_ . '*

'\t10 159) t'l\B~ N32- 1$,RO: ~ r~-~ Qt.ls-. llo-0. IS-.'A--l1P,£t Js~

Tray color code:: "\=1/ ..,,... ~J 0 Analyst: ll'w" *

. . . * . *t~ii>. .. . . . : ..* **

Date: . .

1~* .2;.n JV

C = Larvn_e eight (mg)= B ..._A Hand ~kulalcd.

Analyst: *"

Weight per initial numberoflarvac(tlig)

=CI Initial number oflar.vac Hand c:dculated. iA Analyst: 6\

Average weight per initial number of Percent ~dui:tioit.

from eontroHrci)

.cs:*:.

- o-* '-.~ri larvae (mg)

Comment codes; c =cleat, d;: dea~ fg;::; ~gus, k= lcilled, m ..: rniSsingf.sk =- .si~k; sm-;:; .ilnu$u~lly small, lg= unusually im-ge, d&n= deca$ted and return~ w= wotinded. .

Commeizts.:

Page2Bof90

Page4 of7 sp~ecies: .Pinzephtdes *pronl'etas .

Crteit: 'TvA/Sequo.yah NuclearPfant,.Outranlot, UV*trHtecf* Date: 08-02-16

.1()0% Intake Co~ttol-,Nbn-treifim y z u BB cc QD .&E "FF 10 10 lO l{l lQ IO 10 lO

. r.*o (0 lO tp lO IC. IP 2.'

(Q :l~t) lb 10 ro* (Q.' 10 3

/Q ID re . *. ro IC lC 10

4 ta IO .,

to 10 to f0 lo 5

Jo lo ,,() 10 JC JO 16 to 10 to tO IO ' U.) 10 ro.

10 iO lb '\' 10 io !O f (j

.A= Pan weight{m*,..~ ,..,,,,..............,

. T!";iy colono<Je:: .. . _. . :;;*,.="'--'...;;.;...._;,.;....;;:_.

.Analyst: -.::,-...,.._~~:.,..-.'n;-___.;.;;..._...,.

Date: .._ --....:!!:Z::.::C!J.LU~--'-

B ='Pan+ L111*\'ae ~veigbt.(iilg):

Analyst: . 1114 .. .

Date:. ~=n~1'il .

C=.'Lllr~eweigllt(ing)""B:'-A.

.Hand .calculated.

Analyst; _ _ _ _ _ _ _ _ __ . _

Weight per inltialnumber or lal'.Vae{mg).

=c /Initial numherofllimi.e

o * . . 'I Hand calcu. la*.......

' *~ ~

.*.L<;*.*

. ' .)t*fk> .* ~*. . *~*!\, i.,;9. '>"'"**.

.,..~ Ii.~ .

u;u Analyst: ------...,.....,.--

.d1**:*_*.. . .

o~ . tf' ti

. k*.

\I

' L.:

6" u*

v

Average .\veig1tt: per 'l'eJ"f.:entm!lldi~n initial numbcr'of from ci:mt:rol *(%) (,.1.7..

l:lrvae{m ) .

Commentcodes~ 'c =clear, d= dead, ~g =fungus; k = kiHed;* rn =missing, .sk =sick;; sm = unusually smal~

lg= unusually'large, d&r= de<:anted and reromed, w = wotind~d.

SOP AT20-Exhibit AT20.3, revision ll-01-14

TVA I Sequoyah Nuclear Plant, Outfall IOI August 02-09, 2016 Pi1111!plwle.~prm11el11s Chronic Whole EfOucnt Toxicity Test EPA-821-lt-02-013~ Method 1000.0 Quality Corftrol Vcr.ificnti<in of Onto E11t1'Y, Cnlculallons, and S_t11_tistlcal Aimlrscs Environmental Testing Solutions; Inc. Proltt1 iu1m~rr. _____________1_16_l'I_

No111>1 Conmlionco ""'""n1, l1111!m*l l;ohn1n101y QC rnt1Wl\Yl'llil"t.ftf* Vft111fJt11"1bfrtirJ:art~9 "A;.r:titJ;RU~fi1'1f n... t'11q + l.u.f\.'4t lnl"l'IW \t~li:hJ tmi::l \\'t'lgttt I .liu.l'\'l:-lm:. ~k;1q: "dJihf I Cutffldtnl~h~alli14 \\'rl~ilt/ lt1.llb.l1Wml.*cr Mt;11n,11n*l\'#l :tffd.,,~datlt Qief11rlr:trtlf l"Tml\1 ml!.1.Uio:a frt1ltt f.ur~~ * ~~~l udl:lil,(111f;il ~tJ .. 'i\ uMmturorflif1-*:tt;(rt1:.tl. S\lph.fo>1lf~1nli~tuf (\Jf~...,!l.i!ff'tf~h~ CJno1."'11tcni.ur (%~* ttllt1.. 1n11111~r11f t.-itbJll*P.~"\J(-.IJ... l!J.'U -Wl\flt.f t,;\j

,.......,*!fi.ro;1J4,.~~j r~.n'!te('f'l:l pt~ul~r*llwmj lat1i~~~1151'

-t'C 10 10 15..14 ll.ll S.87 t!.SM7 f),5$7 Cu1tt~til 1 . **no*** O.lSS

~

10' IU 14.114 20:19 -*SS o.m IJ.6 100.0 0;$Zl ll.6 Nol n11plir~ble M2S

-~

t\lotl*ft1'U"d llt 10 IQ lf\.$7 I

Ff *.1q - IO 16.0l** U.4~l *--*

,\ *11) IO 14.'JO* O.S6S

-~

CblllrOI, *R I() to IS:~O 20.Jl 0- o._51~ it.6 100.u ti'518 8.6- Nolojlpll..,bl*-

l*V*ir<nltd c -Ill IO . IS.82 11.l(I- 0.548 I) *IU. JO lH? l<l..17 _0*178

,.; 10 to HAS '2<1.ll *US* 0,488- 0-436--

- .~

I' -10* 10 -J(i.70 2tll UI'. OA61 O.SU4 1,$* l.&

10,?% 11504* .7.:f IGM

c* 'Jtl rn* 14.92 lU,08 5,16 . U.SIG--

11* JO lU JG.I&. ..21.67 S,4!l" Q.~49

... *10 10 ----* . u.oo 20:87' 5.27* U~7 2M'lli J '10. IU '1669 .ll.l*I ..A.~' 0:468 9:u VA6S-0.430 100,u !}~Gii 9.0' 9;7 K 10 10 16-.02 20.ll . 4-1()

I. .JO" .'Ill 15.88 -~(),J7 -4.4~ M4!.J M49 ..

M JO IO 14.70' - 19 SI 4:81 OASI .. .MRI 421!1% N* .rn. IO IS.SO lD,5!).. $..1)9 0.50~

Q;5!4 4.9 O.SO\>

ICQ.O 0$14 4;5 Q;9

~bl' 0 to H7 .. 0,517, t::I:=

IO-

.'I' to *IO. 5.38 *OSlS n -10 *10 -*-4.7lf (}.470" -

R **10. IO 19.l!J. *4,9'} -o.:l?<>> *0.*199 .

SS.~~*- MU 6.~ ?7~ (1;;161 .. .6;l 10.8.

-s 10. --Hr 1~,~ . 20.JO 4.ll OAll U.4H T JO ,u -'IS;45- 19.~4 4A~-- ll.499 OA49 u** 10 Ill *16:13 20.19 4:66* Q,.f6(i 0.46fl*

m 100% \' 10 10 l.1f!7._- o.so2. 0;51_~ 7.7

-

Q.SOl- g'/;$* Q.501 '5.5 *J;~.

w-* ---* .. u34 IQ*. *10 i~J9.:* -11.$)4 X. 10 .9 IS:76- ,O,H7, 0.50!

I' w* JO .o.479 0.479 tllb.%lnt~ico

'Z 10 10.* .u,s11 11,49~-

O.Sll 9M o,136. *u G;1

. ,\A-

.Hll 1(1 JQ.

10

.9 IS.

15.8 0,495

_o,srr "*' -0;\9' 0,461)

~ . M$Q:* Milllriunq_ si.wi(r...,.,p1rr-

o..Hntll't MSP ""'"~' Q.OilU!l l!MSI>*' P~tW._1 Mioimfl!li Sii!rll!l..,it Dffl'oie..ccr

l'MSD1 II.Ii. llMSll h n-u!liorlesr prid!ion, Th: PMSD_ iJ~-oil*lmum l'dte<lll dill'l!ttt1c~ ti<iliwen 111~ co_niiol iUlil "'""°1"'1 IMI tM be d<!<ta;lida1D1iilj~ly 1ilinlll1:1111 111*~ ~.01*~ni=n1 ll!l!icil1 i.st hii*i<*1_

!lun.ttttt't lltSP ~1101 O.Ol!l-I l:CW<t l'~ISD b0u1id d.1tri11lni.'il by .USill!A (lofu pmc<nl!O) - ll,-~,

1!~1$1>1, 9,3. U~jWPMSU bound tlllilliN~ by USf!PA(90ib PPf<~il!~ "'JilW Lo.vctlllld UP,pcr PMSD b<iliu~* .Wi*ililorittini:d llom 1111!.1_01~ nn*~oiilc, rt$li~lill*lr. Crl'MS!> m1afrom EPi\'~ \\11rrrri1i:iioti!lrl!l<X)'VniillliilfjY sl~dy (USllM.l-041~ USfl~A. lCiOlb).

lJSEPAc lllUI~; lfJOlb, 1'01o! lt*Pliit! _lotoil.iboi;i1uiy jl'w~llily_S\111I,;o ofi!PA Shon*l<<m Climniond AcvwWholk ll{lj...,1Toii10Jly1'"1 /\foll*Olls-.Wlun;.,. I md~*All!f<ndilt..llP"'Bl_l*D'°l*©t.nd_llP,h*B:u,{Mll~00_$, US lf!\VltonlJl~lll.t l'!'ll>'.<*i1>1*1\Utl1C)l.,C:ihl:ion1111;Dll,

TVA/Se<fu~yan t"fu¢1ear flaJ1i1 OutfD:lMOl

.. Au*l!st'<f2~tJ!)-, 2016 Stati$tical Analyses GroWth 16" Test:!µ: .. f'pFR9R . . Sample !O: TVAJ SQN0utfall101 Eni.foaJe;: 16 L\a:ll.IOj ~,EnVfr.Te'stlri9~ .~1'Yf'~ DM!i-Olsciiar91:-Mo71it0ri119 Report

.Saf!IJll~ o~e: Aug!,fst 2o1 s P\'(ltcleQl: fWPl'f&;EPA~8:!1-R-l:iUlt3 TestSf?etjes; pf'.pi1nej:ma1es promeias

CommerilSf coritr9J-~PN o.sir;;o o.5550* l).5250 0.4230.
P<mtrol'-OV o.s!>So 0.48.20 :0;54811 o.47ao*
.t-9*1. !l.4880 G'.461.!l n.s1 * <>:s4.
21A o.s270
f!,4650 *tiAaoo; <>;~sd 42;8 0;4S'itf p.5<!~(1 a.astir o.;52ao
i)5,5 . O..f70'i:r lM!lSO :f}.4319 '-0;44\'lil*
.1ilil o.:ii$6o o:S026 n,s340.. o.*ooto*
1~* . 1,{47sq .0;~1.1~ ;0,,495,0. OAtlllO
...... -* ~
....Tmnsform:*tJntranstormed 1-Taifed lsotoQie*
.dono,.o,s .Mitan N-Meari Moan Miil' Max .. C\1% N t-Si:Jt. *eritical MSO Moan. . H*Mean' qinfl;t:IJ~N9fll 0.5225 :1.0082' 0;~5 0:4230. 0.5870 13.589 4
. Corilfol-JJV . o;s1a3: 1'.0000 0.S183' 0:4780 0.5G50 s:s~3 4 D:51Ss 1.0000
j0}1 0.5035 O.W1S d.5035. p;4610 0;?-490 7:4\'.lS 4 0;593 2.:410 o;osoo .<i.5035 o.971s 2i.4 i>;457s Q;902S ~.467,8 0.4300 o:s:tro s;sa'i 4 2;030 2Ato o.osoc:r 0,4908 0~9469 42:8 1)£130 0;9913 o,s1.sEr {);48~0 o;s:i1o 4,819 4 o.1a1 2.410 o:oeoo 0:4908 0.9469
'e&.6 I)~* 0.11919* o.'4G~s M310 M9!'10 ;;:aas 4 i251' :Z.410 O.!JijOO 0;4Sl5 0.!}291 100 Ct5008. Q.$!>52 o.5'i.ios 0.4660 . q.5340 5;547 4 oJ*oa 2:4~0 C;(lj'IO!'l. 0:4815 O~S.291 tn~e DA!iss P:~3S3*. oAass .o:.c1() o:s110 4.491 4
a,it1cal 0;,941.98 o;aS4 0:26917 -1.!)903 1"52143 :ts.oses*
0:10128 ... 2.4469'1 MSOu MSD MSB MSE f'-Prob <ff 0.05997 o.nsn o.0022s. 0,00124 o.1ao4s s. 1a 1.lnoar lnterpolotlon (l!QO~eS..mpfos) i:>otnt '% so* wt..1::4EXp! . . SlioW. . . .
!COS. 20.06?' .
1¢10 >100 IC15
lfud
>fOO
'>'100 t.o -r------------1 teas >100 0.9 Ll.':41) >ioo 0.8 IC&l :>100. O:'l
o.s:
c .
.g_ o,s
&b,4
0.3 D.2 0.1 * ..*.,.
-o.:oi4*~'~:;:*~:.,,~--::*::::~-;:__~J 150
0.7 .. ..---------------------*
,.

'O:~ -..1--r--,...,1.~U:.:O.os1e~
31 ..* of slgnmcanc~
f 0~4 ..
.q*
l:l"oii l:I .
Q;i' 0.1 Page 31 of90
TVA I Se.quoy~~ Nnd~r. f!lant, OutfaJI 101 - I~t~ke A~:gust02...,09, *20Ui
",,.., ._ ~.. ,:.~~ {~ **:.>:*, <:>
SWistical. f\nalyses
} E'nvlron,...nt~I Tostlm; Sotin:.,1nc; Larva1*Eish' Gt.oWth* and Survival. Te5t4*Qay Growth.
Start Date: 8121201.6 Test 10: PpFROR . Sample lot:; "NA /<$QN Outfall '101 ..,; Intake .
End Date: 8/9/2016 Lab 10: iffl~eovJr.. Testlng Sot. Sarnpl~ Type: OMR~o.t$t::tt~t9e. 1\111),Q~onng Report Sample Date: August2016 PretocoJ: '.FWCHR-EPA"821..a-02.-01a TestSpectes~ PP~Piim:iph~ promelcis Comments:
Cone-% 1 2 .3.' 4 Control-NON 0.5870 .o;ssso ll52SO * . 0;423Q Controt~uv 0.5650 0.4820 0.5480 0.4(80 10.7 OAS~O 0.4610 0.516o O.S4!10 21.4 O.S2.7Q PA~.O 0..4300 0'4490 42.8 OA810 o.soso p,5:m> o.52ao 85.S 0.4700 0'499!1 0.4~10 0.44!}p 100 o.4660 0,5020 ci *.5~40* Q;5Q1o.
!ntake 0.4790 O.SHO tt495,0 bAS,oo Transform! Untransfotm~d. *1~Tuiled Cone-% 1'illean N-Mean Mean Mlit * .Max. *..t..Stat CrlUcal *MSD.
Control-NON o.e22s 1.ooe2 0;5?ZS Oc4~0 . 0;5870 13.5.89 4 Control-UV 0,5183 1.0000 0;5183 0.47~
0.56$0 8,633 4,.
10.7 o;so3s o.971 s W?l:i3S OA6.10 0.54$0 7.496 4 21.4 0.4675 0.9026 0,4578 o.;4300 .as270 *8:981 4 42.8 0.5138 0.991'3. 0,51$8 oAa1o q;s37p 4.819 4 85.6' 0.4623 0.8919 OA¢23 0.431.0 Q.4990 s.32~ 4 100 0.5008 0.9662 0.5008 oAseo o.5340 5,547 4 Intake. 0.4863 0.9383 o.48ss 0,460\} 0~5110 4.491 4 Auxiliary Te$ts . Sfati$tic Critical.* Skew !<.Utt Shapiro-Wilk's Testrooiciares normal dI~tribi..ttion. (p* >. 0,01) Q.9~37 oz7~
F-Test indicates equal variances {p 0.27:) = 4.19692: *47:4683
.,.Th~e....;c~o:.:.nt;.:.;ro;:.;.l.:.,m~e::c:::a;.:.;nc;.s~a;;.:re"".n-.;ot;;;
....;;:;:;;~19;:..;11"-ffi""ca;;;:;n;.:.;t""ly..;;d;,;;lffef
...* :;,;..e:::;n.:,;;.t.,.,*(ei;...'.-ii :.'""'0.,.;9;.;;:2..,l-...""'__,....,.'""""-.....-o:;;.""1b;:;,.1;;,::29=-*,_,,,,.,...,,,__2"";""4""'469;:,;*.*.*;;;..;1'-*--==,,,..___,,,...,,...-..,,.....,....-=-_,. ,
Hypothesis TtiSt {1-tail;0.05) .' . . . . . . MSDif. MSO.I? .. MSS M$5 F..'pi'.i)b df Homoscedastic tTest h1dfcates no sigr'!ifi~r\~ diffef;?n~ o;04837 Oi09333 0.0"0205 1;LQ.O;J24' *0.21599 1, :S Treatmel'Jts vs Control-UV . /
\).7 M
...:t o,s.
Ji;; s...T~---:::::'f":'":.":":-::+** 1~~t; 6.05 lelll?l
.. of slgi'!.ifrcar:u::e e GA
(!)
Ill 0:3, c
t..
0..2 0.1 0
z > 0 0 *=t .o
ii:
0 g
.;, e c: .. 0 0 0
O Page 32 of90
Page5 of7
.Species: ,Plmeplurles pfomelas Date: os~o2.,16
client: TVAIS¢guoyah :Nuclear Plant, Outfall.JOt,,UV.;treated.
Dail
Cl1f!i1ilst * ~
measuremenls onl *.)
2 Concentmtion:
CONTROL ov::trea(ea:
i0.7%.
42.8%
85.6%
... Hard1Jess
, (m" CaCOJill Condui:tivity
.(µmhOs/cni}
ti,Mf%* *A:llmlinity Intake . CaC03/L)
aardnei;s
..Cm . CaCOJ/L}
Tern ern:ture,{"C)
Temperatures perfonnCd at .the timeoftesUnitiaiio~, rcnC\"l'l ortenninatfoo by the*aaalyst.iderttifie<l iMhe paily Renewal lnfo.rmalion table loea!ed on Page l.
d._lka
ity1hardn~and total ll:sidual chl'orlrte performed' by the analyst idei,dfied on the beqch sbeetspecifie for each analysis and 1ran5cribcd to this bench sheet
....~~"' . * . on
ToJat residual i:llforlile :WllS performed lion"ttcaled:Ouefall I0 !. arid in lake sample$;
Page33 ot90 SOP AT20 - Exhibit AT20.3, revision 11-01-14
Page6*of7
Species: Pimeol:ales promelas Client: TVA I Se uovan Nuclear Plant Outfall JOI UV-treated .Date: *08-02~16 Concen- Parameter tration CONTROL ....,..-=--..~------,.__----~
UV-treated J0.7%
21.4%
42.8%
85.6%
Conductivity
(µmhos/Cm)
Alkalinity 100% (mg CaCO;/t.}
H,u*dncss (mo CaCO;IL}
"TR chrorlne (mg/L)
Tem erati.11'.'e *ec)
H {S;U.)
DO(m 0 Ll Conductivity
(µml1osfcm) 100% *Alkalinif;y bitake (m°CaCOs/L}
Hardnt:$S 1mo caco;1L>
TR chlorine {mg/L)
, 0 ** eratures perfomred at the time oftcstiniiiation. renewal or ienrih,1ation by lhe anruysr idenlifi.ed iii U,ie'1)~_ily Renemu.lllformatien tab!~ l6ca!i;d on Page.I.
Art~ialin y' hardness and. total residual chlorine performed by .lhe analyst identified on tiie bench shectsp~ific t0r eacll an:alygls mi4 Jranst.i:i~:IQ th.f~ b~ sheet
"'"13~****' . Total l'C$idual chlorine* WI!!! pcrt\lrme~ on non4reated'.Outfalll0! and Jiltairesamj>fes. . . .. ' ..
~ . SOP AT2.Q.,.. .Exhibit AT.i0,3, revisiQn ll-0 H 4 '
i~ir:~, ¥ ...... , **
.. v-*><* '"<"* "'*****,..
)*t* Iii .....~~
Page 7 of7 Species: *P.imephal~ prorneJas . . .
Client: TVA /Sequoyah Nu:elear Plant, Outfall lOl, uv~treated Date: 08-02-16 D~y
.(Anal st identified.fur each day; erfOnned. H. DJ). *and. eonductM measurements only.)
] 2
_AJialy5f
Concenttation Con trot Non-treated I Initial Initial Final Day (Anaiysndcntlfi!id f¢t eacil day. ifonni:d R.IlO. ilnd*coildilcuvit *measuremcnlS only.)
Concen,,. * .* Param~ter tratfon:
Control* DO:( !i/L)" *.
Non-.treatci:I CoruluC.iMty
(µrn11os/cm)
"Mkaliniti*
Jnig cacO~t.)
"Temperaiures~perfuml¢dll_tctlle timeof't~~iriitiati()~. renewal*i>r ierminatfoncbyUie analyst: identified in the Daily Renewa1 I11!0m1aiion table located on Page l.
Aikaliility; bm"driesS:tli!d !Qlal re:sfdual clildri~ perfomied ~ytbe ana!ysi ideritm'ed on the bemib sI1eetspecific roreach anal}'Sls and transcribed 10 this liench sheet by: . .
Page 35 of90 SOPATfO-Exhibit AT20.3, revision 11-01-14
TVA I Sequ()yah Nuclear Plant, Outfall 101, UV-treated August 02-09, 2016 Plmepllllfes promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method IOOO.O
,. L'.J Daily Chemical Analyses
. ) .11nvltonfllel\lill T~llng SoluUons, Inc. 1'111jcct number: _ _ _ _ _1_163_7 Conccnt i'nllon Pa r11n1ctu l)nv Q Duvl Dnv.2 ...DuvJ .Dnv.4.. . Davs D11v6 Initial Final. .lnllllil Finill
lnllhtl Flil11I h1ltinl 'Finltl Initial Ffi1ill lnltiitl Fl1111l . lnltllll Finni lnH.tSU\ 8.09 7.82 7.88 8.06 7.80 '. 7.76 7.99 1,78' '7;73 7.116 DO rn111/Ll 7.8 7.8 7.S U 7.8 7JI 7.8 6A 7.8 7.S .7.7 7JI 7c8 7.9 Con1r41, Condnctlvlh**<umhoslcli1) :)Oii ;,o;' . .. .318: ::*:,, *
318'.. 320ii.*'*'
Non*lrtllltd. AlknUnlty(m!VLCoCO>l 59 ./'*,;;j,;:;*::; ;>*.:,:., ,::* *;,~ ;,:: S9 :.. *. ***** *' :.**.::- *, :* '
H11i'dJic!S(mglt.CaCOl) 811 '..'.""* .. :*;::***:,*.~ ';;::*'* ;;;; ,, 9(i* ...... *,'::.::::*:;T"'"* 90 .,.,. ' ' .:' *:.* \ ;' "'
Corit'lii~
ConductMfytumllos/lllti\ .*301 : ::,;;: *<""' ;ns ;:1;::'"-'*'s:;c:;: 312 " .... '322 "'.'!!>'"i."*' * !!OS". 311 : ::*:*>'0/*.: * .. 317 '" * ,,,,.. ,..
uv~trenfl!tl. Alkuliii11y,(11iglLCnCOJ) .:59 :;,:~*;::.;,:;G;;;:;, ........ :c*,::~:'i* ;2i;;j:,*c:.1 . *59 ,; . ::~; . :Ao,)..:.......,, *'***'**'e}.* .ss :-.~**'"'*" ... *:*;,,,,, ,;:...;,,,,.L::.,;'i,,.. ....... , .
. Hardricss-(111uttcaa>.,)' *.oo ...~,.. ,., *,:'***..*":'" '*" :**" .. " *sa :>:>:**< ....,***: '*':* .*:-. .,, .... ,.* ** .,.*.. * !lU .** :::. <':":.'.' ' : " * :,. ;;; . >;:'.":'.':': .*. * ;.';':.:**~.*;o:*1 > .,:./
. 'fcmncrnture i"Ci 24;l! 24.5 24.7 . 14.5 24.7 24.6 ' .. 24.7 2~.4 1111 tSW 8.04 ' 7:84 ' . 1.91 1~16 7.88 . 1.12 1;!14 7.49 8.02 7.85 00.Jlll"IL\ 7.!l 7.9 7.9
. Coniluctivltv rumhos/cmi *3.1f *'"
j4;7 24.8 24.s 24..7
. olUSUl 8;04 *7;84 ' 7.!)2 1.11 1.88 '7;74 7;94 .1.sa 8.00 7.74 7:84 7.85 1;ss
DO 1111u/L\ Vl .*
1,8 1.8 7;!l . 7.8
7,fJ . .1.ll :M 1.8 1,9 CanductMtv CumhlillfCUil* 28.S ~- .,. . 2!14 ., .. " ws .,;,o,:,, *..,, " ' :298 :.*:,,,:;_,, *, ... 289 .....',/*.**:c JOO.**
s,01 1.Bii 8.t.l 7:9. 8.0 '.
211 . . ,.... , ..
.>'71.9. !/,9 'Vil . . . . 1.9 .. ~ 7.ll ?,9 . .7.9 'S.S 'M :7.1 ~.l UJ SJ M Co'1iiluctMty{ullilto$/en1\, .2:u J.*..., ,.;.:.:;> *227 ,,,,,,,.,. .,, 216, *.,,,.;,,.~~ ,, : 226 223 ::0 :7,*'."";< ' 224 l ...,.,, : .:7.: "233 ...
100%
File: $qri1111,Jl8Q211lcheril:xlsie Entered by: J. Sumner Reviewed by: -d(-
~-
1 1~
1 Page i of7
1
-1
.($11uuc.Wio1ecEtfiuentT¢riclti T~st:(EPA~21.ik~o2..oiJ*Meiliod I002.0)
$pecles; Cetiotlaolz1iia d11bia **
1 Client:. Tenn~*-*va1tey: Autli~ttv C{)unty:* Hatniltoti Facility: sea:UovakNuclear/t>nutf- Ou~tl: 101
.NPJ:>l~S'#k* TN0026450'. .
l ,,,.~j~ef#;_ .
d-~~"'."\
olftmeitls.:
-1 ~t,4'
".53~*
... AZ~*
l 1)70 85.6 2140
. . .100 2SIDl PilUci'!~v.olu~'.<ll'li..fl :~zs; I 1~6$ J l43() '360:, '*
1 j)
.1'ott11.\'*.<mi:f7] ;~90 :r:~wr .*L 2sht'J, 25.QO J.. 2$00'.
.TesFoi/iilnfs'ili-SilJuc~ zitfi>~htaiton: *l Tett bifermtrtiom I t_~ I-
.~.l4:'ltoi.1rs;.i>Jd ....
~.ut"'~~~Q T'a-fil\~$ *1 ~1?8tot~~;itidsttetr I.. . ..~,...,....~~*~---~-~--~'."'.'.'f:'~~;u;*JJ~~-~-~---~--~*-dji**~x~*::*::*:;:::~~
l i.c~1..
. ... . .. . . . . :- i!OClition:
l
1 :.*1 .::.. t I YWTbiltcli:' I tn*l\J*l~
1* HQim~*--~~t:*tH&\AU~~u'"'_ - r *!::'~~-=-. ***~=t: ..a*U:*: o1*~Uf1"'-UU.Ulw- .. "-'°""* *~*.I* ~~le1~i;1Slftit1t,bateb.;.
er\*~**'-
JJai!X re1tew.a.li1formiliflm:
l>jtj .D:fte Ji 'r~tinitia~iti)Jn~dfee~i~ MH'sw . *.Snmpie num'bers used Analyst
*
ie~yAJ ~$!* feecfi?g, of *l.ffltch.t1$ed Ouffalt'.101 I Intake

termination: time.
.o r og"f12... 16 : l~C>,Ol*O~
j, . l(j~3~1*~.
z 108'~<14-161
-~** l 08~1'6 .
.J .4 .
I 0$"-0'li;-:l{fJ
's . I, *P1~ It!;'.
6: . . I .og~*l.6 ..
1 1.,o~g;.1~
_J
1. &nii-dli1zftitlfltilfo1z: $1lrh,11ll:UJi <!/test endpoints:
,CbrilMl-"l :Conttol*.2 .\c(@fanec i:rJtena :
f
%ofMP!e,Ath.il~ .t:\j:, 01. ~*~g% 17"-dav 'LC.so >H:~1.
%:AdUl~hav.fug_l_'nl*_a}Qotls; .: .001. '1()01. 1::80"/a> 'NQEC .l001.
%.Mortality,;, ,, . ..aJJ '01.: ',:S;20% ".LOEC '>1001.
Mean.Q.fisMfi~ai~:, .30'~(;~~ Ji)~S ~ 15,0 -0.tfsp~i(lg/~1~ l C.bV >1co1*
. %CV;* .....~1\1~ '4.'11. <4():,()% !Cu >u:io1.
J
Page 37 of 90 SOP A Tl t~*E:<hibit ATI L2, revision l l-01 ... l 4
/
Speci~: .Ceriodaolmia duhiit Client: TVA / SeC:nioy~ Nudear Plant, .Outran 101 CONTROL-1 . . 8..ilrvival <1:~if JJ,e.pruef11<:tio11 :P/Jfi!
.Repliea~ *~11.m,ber Day 5 6 ., 10 Young prOduecd Adiilt morratitY * **
Z. Youngprod11ted
Adult inoriallty J
Y<iuqg pr(,lduce~
Adttlt inort:ilii:y
f 6 \'ming producci!' .
Adult mOt'~lil)'
7 Yi>,ung pr9iluccd Total young produced Xfor3"' Broods
. Co11cen1rali.01fr: ..
CONC: 10.7%
1 Yoimgprod.u~d
M '0 .. a c f'r ~* (3 . *n .*. '(\ .. 0 .. 0 Aaliltinorrauty L.. :*L.... . L L:*
C * * .*L '. * *
L *** C ~ L *L.
2 Young produced* r\ . . i"\. A *.* (j' . *C). '. .' O.*' ' 0 0 *y 6.:; : 0.
Aauit mortaliij * * \.: : L * ~ * : '\,._.;, c * *'\.:..... * * *~* ****"-*** * 'L *J ........
! 3 You~gproduccd *
O ' .0 . . .: :("\: ... : 0 '{) *.r;,. .
f"i [') .. {\ . : t'\ .
AduJtmoraaliiy .L; *. L
L.. *\.-
1
.**\;;:_.. L
L.. ** .L:. 'L \,,.....
4' You11gproi111l'l!d . ~*~ . *H .'. s* .4 **. l{ q ' ' . :;.. s '"-l
.J AduJimorbi~IY ,; C '\........ 4 *. *t..._:;.. * \..-. L .. L... L- *\..:..,,..' L
5 Yonngprot)uc1:tr . tQ . \"~ .. t1 r~ f'1- .. tt.: ,,.__ l 6 . H . ] l ..
Adult m0rtality \_ \..;_.
L . ~ \....:. *~. ..**\.;... L,.. l,..
i'L
.! 6 . You1,1,g produced :* *o o* (J. . {\. .. .. a~ .' 0 *. 0 . .0 . A . ti
,.\dultmorii!lil}' .' \.,-. \...._.*. L.* *L,...* '-,.;' ~i \,...;,.;* L... \.,...,, 'L,',
Toralyoung.productil: * '.3\ 41;, .. ~;;;, ' ~S *: 4t>.. 3o'$ l ;~ S j' '2r~ :3!. <~~
FinalAdult.Mol'ttlllty. -~ \....;...' L * ..... \;...;.. L.. L '.L: . L'.' 'L . "L :*: "-.
Nt>1~: .\i!ul1.mcrta!ily .q..-r.vo-.*o""dt><!l**~~pllibra~ ~J¥.elm>iiil"!:f!.1 ~~iwo~~.~Q= "'" oV<t(o""""'il.c:inied OY1!1'"1!h.iidulr&IM.i>.~;
* :Concenirailott;. *
.'%.MOrialiry; ** * *
01.
IWelip pmp~nglFe~e::. **3~ 1.._*
,ra;Retludio.n fromaontri:l.1'"1: * *** ...,$."t.7;;.
Page3Bof90
Page 3 of7
S~es; D!iloiliiD!mia .tliihia . .
Cli~~! * -rv°A1 segyoyah'NuctearPlknt, Outran 101 I)ate: OS~OZ-16
(:ONC: .21;;4%* .S11tvivui <<nilReptod11ctio1z Data Rti:iftcafenumber
.mY:*
1. . . .: 2 . ~ 4. ~s . 6 .
7* s 9 10 t* 'Youngr(ifoi!u~ * *~. N C::,
0
0 l\ 6. *0 0 0
~ultmorJ=ilir,t "L . L .. *\..... .L. . t_. \........ . L... *L. L. L
~: ':Young*priJd\r(:cf) . *0 . . *o () ' 0 . u 0 .0 0 ('t n
.Adtilf morni~ t.._. . .k, '- ~ L L.. . L.. . \....; L .. ..L
~* Yourigpn)tlu~ r)* *O* :{). n o*. 0: . c 0 D 0
,i\d'illtmortaUtY ' .*. \...:_, . x,..... .* ~- . \_..,.. * *'\,\_ . . L . :. L *. *L.....
L,. ' - -
.4 Y!un&pfC1duc¢ti* . *. *'-\ .. * ~ C::.., ~** 5 * ""\., .~ **"{. : *~ S.
.*; -~utt~9rta1i~ \.... * \.- *L \..- L .\...... :: L L. . L. '- *
s Y0uilgproducc:il* :..\~. .\0 \ "L.* 1:"1;.. \ 1.* \":I.\ .; \ 0 \,_ . 14 l\
Atlldtlli{>i:tilllty ~; '\.... . *.~ . ~ '\,._. L. c '\;__ L '-- '-
6 You""t1"*1it:~td D.. .D. o* .(')' 0 0 ~ c *o 0 0 A.:duJ(nmr,tnlity * *** \:....... . L . L _, L \.,..;,. "-- : \........ ** ~ . "'-- \.._
*1 Youngpro,auccd : l"l \~ . '~ \.\. l <<;.. .\"\ \(~. .\ -s . l~ *S
_TOtal~Jlni*1JJ'ild~ee£l* . : °!>\ . ~1... *' ?>>1.. '?:.:\.\ ~'l.. $lo ?)u o\ ~'-\. a\
Fiil:ilAdwt:A>lorlality ** * .. '\..- *\-. * ' - - *"---. \......-- .* '°'-'** :\....... * . \,,;,,,:. ' \._ * '--
~:*Mtili ~ (L:-~'l>"'dad), SB=sj)ljj booiiit(~fobtiiofipll!liClW~*iiWi>dojs)jCO <;>"""' ova: (o*-"""'ciriic4cw.rWilh:fflllt ..,.;m,, tmsrcr);
. * * * * *11tieit1ration:.
1-,,.,...,..,-"-,..,,-......;..~~~~-.---:....---I %Mortality: 01.. I .%Redtictfon from Control*I: -s.eo 1. 8ff!Vh>ala,1dReprod11ctio11 Data I .9 .IO1. :v-Ouagpz.'OdJicecl n: .. 0 .... o.. n 0 . **.o 0 a u 0 l *.. ~u1tm1t~t1ty;L L * 'L. L LL* , *L . L... L('\ L 0 L ~ .(*'\ ("j '-. .L '- .. S .~ I .. \~ ~ '- '\ L \.... .J t"l 0 \.__ l"\ \~ _J 3""\ 3b ~ '-- Nf!/tf< *Aduli~' (L:-IN~'i>""' ~ SlJ"'!plil brn<iif<sinsl=brcod 1jili1'bei~lwo ~1. CO"'cmv<J11u(o11tmint< c:oniod 0vcrwl1fuduh dwlM ~). Coi~rqlioil: . .*% M0rtality:* 01. Mean OffSpringlf'.emale: - s.c 1. Page39of90 SOP-:Atl l-.8xlllbitATIL2, revision 11-01-14 l Species: CeriodnpJmia t111bia Client:*. TVA I Seguoyab Nuclear Plant, Outfall Hll Dat~: os;;oz.16 CoNc: 85.6% * * ** *** *Surviv.al andR.ept1Jd11cti01z Ditto Day l 3 lI .... 6 Finni Adult 1\-JortaiitY **\;.;._' . **'"-'* .'. *~ . .'\...,;.;. ll'Olri: AdUl! ~mlily (L "U~ D "delid). SB*='sjil!):'lit~ CiiillB!~broad split b!:C~!Wiiiliyi); .CQ "i2!i *:iimtfu1.,,,;d~ Carn.:d owuw& oili!!t~ittntfet)~** * * * * * *, * *. * * .Co~lce11tiittli!n: *.' %.1\41)rtali~: 01~ __ i\'iean dtTSpring/Feµial~ * ' .:~. l CONQ 100% 811ritlvtil mzdll.eproduction i>a_ta; f Day 1 Young pi:o,duccd. \....... c* .**L . I 2 fl.l.d~lt iiwrtnlity, Y.riung produce1f -. .. \....: .. L 'L*. . . p.., * . Cl' * .*. * ,/S:L 1"\L Ci.. *\;* '*,, t 0 . .**; *.* 0 . 0: . ,. . :C\ . O*A~ult mortlillly ** ; 3 Yo.1mg 'prodli~~d* ** J .. Adult morfality4 .. Young produced Aduit mor:tali!Y 5 You,qg: prol.lilcc:d Adult mortality 6 i'oung.pnuluccd ', .o :** o **o .. a . ("). f!\*: o .. 'f) _ n ti*Adult mortnlJty 7 Young produced 'Totalyoungpr~~uc:cd * 'f . Final Adult* J\>lortality NDk: Mot1 il!onofijy :(lo* live. tf~ dead}.*SB.f' *Pi! !!'!"id Wltglc>briioih~t.l>Otw~1wp d;im CO="""* iffi:r'i'O,..,.;"" c¥1\'c40¥0r\\11lniilllfl lfllrill1tir.DW"1); CiJ!li;enir..a(ill1t: .. 01.* '2i*:">I ....... .. ~~-, *. Page40of90 ,~i,s: . &rtgdni!tnia ililoia .,. . .cfien"(i TVATSeq\tQwh Nuclellr. i>laitt. outrarJ lot .* Da~e: 08-02-16 ¢QNTRQL;:.2 *Sili'Vivtil a/illReprotl1ietitiizD<<tii RepUcate mnnherAdultmor;_tali~y , 'L :L ... L.... "\...... .L .L. l.- .. L.. . L L. 'You~pl'pdj¢~.' ' *O* . '()** ' o. ' (") .. f:'l...... :. . D*. . :~-a* **('\ . ' L} 0. - 1 :4 . ~ul.tmor~litY,.. * . *\_-i Y~11111g prod!tceq *.-:as ** \;,,.; ~_ ., : _?;..L . ' \...,;..,t _~- .. ' ""- \..... . . .* L* : \........ ' :S : CS -~ \...... ~A~nlt.mort?Iity . : *~ ~. . \...... \;.,..\,. \.:... * *\..._ "L. L '- '--'Y~ngpi'OcJJJcea * .6* * (j* *Q . O .0 . 6. b o O 0 Adulr~oitalil)* ... L,__.: \..... "L. 'L:,_; : : ~- \...... , ~. \,__ '-- \.__7* -Youngprod~~tio 1t )"'\. . i~ -,~--lq, . l~; : X1. J~. * \'1 \'5 I . *; . "': Ji'.iiiat.AwltNiortali&'2.~ ; i -~:?;) . -~\ : "'1.C\ . ' 0 \ . "14 ~.,_, --~\ :.. \ . ~ 0 .. N'tlk:*,l\illllllllOmli!Y.J (J..*"'!Wc;P"'<k<lcl).SB .. ~J~~fsil!ffelmio\lspf1tbm~.IWQdO)'S);_CO"'<:m:\'"\'C((oll$pnnscnrri¢~*ehYilhoifiUl4ui;nstramfot), i * *Com:entraiimi: .I . .8tiivfval artdReproili1i:lion.1>ata RcoJicatenum6cr .* * .I 1. . *vount,Ptqdu~d f). 0 .. 0 0- .. 0 .* 0 . 0 :n 0 ' ,\dult mortality** .. \;.' . L .. L.... L.. . L. . : \_,._:: .\;..,..;.,L . . \..._2 * ** * .)'oung.tiraduced I'l *. -(\ f1 ' ' .. * ~0 . CY * (). .el 0 .o _] . . Ail1dtm~rt~lity . : L _ .......L.. :L.. . L . : . \:.....;. *. L., L. . . L . L .\ 3 . '"V~ngpt0di,ic¢11 -'.r') £1. Q.0 : *o * .Q ('\ . ti: .("'\ A~c~ult~o!'fli!~tr . -~-- l "". L .. L' .. ~ .. \.,...;; . . \ - L .L .\_.... L .4 \"oungpr-0d9ccd ,c:;. . *** S -l .S i <: **~* *"-\, .\,p \p. )Aif111tm~rfilf!Q' .. J,:.:... :t L, : . L .. *c. \....;, L. . L *L L . L S "'. i'Oul1g pn.i~~~ J;f:) ; . J,C t"\* I ~ "L, i.. \ 1..... l°4 . .. tl..... pa,. \. "'t. i1-J 6i\~tilfmortiil~- .. , L-4. \.-.. ** \...... ... *~ : i. ~-V:-Ollt!g produeeil: n : . . O . . ,(') ..L r:-i. . h \..._;L~'{). . *ts 1'. . _() \,._ Adultmor~liti *_:c :. \:,.... '\...._.: \.....;. L ..> 'L . ..L.. *\........ \...,.._J .Mire Ai!lili~!ii:Y: °""'fl\le.D"'"""~'" ss ..;splilliniodJ~hi'aod~ bc1we<n im>cla,y$i. Co*tanv Ovi:r-.<orr,.,,;M,,.nicd4'-.r"illillllult duriri~ IQmfcrl. Ct>11eentrnJio11: .% Mortality~ 07. Mean Offspring/Female: ..J %Redueinon from Contro1~2: -1~ ..\'1. Page41 of90 SOPATl'l --ExhibitATlL2, revision ll-01-i4 TV A I Sequoyah Nuclear Plant, Outfall I 0 I August 02*09, 2016 Verification of Cerio1/apl111ifl Rei>roductlon Totals Control~! * .. 856% Duy ' :Ronllout1h1umh~r ** 1'olol Uny Jt.inlle.iui ntllllll*"' TolUI J 2 3 *4 $ Ii .7 *a 9. IU I 2 3. ;I s 6 1 8 !) . II) I* O* il :0 il 0 *tt *o o.*. 0 0 0 "I 0 0 *o. 0 0 0 0 *o 0 0 (), .:l 0 ,() * : :o. .o (). "():' l) 0 0 ll 0 2 o*** ll "O. "() 0 0 0 0 0 0* il J' o* 0 0 () 0 0 0 o. 0 *a .. *a 3 0 0 '() 0 0 () () 0 () (l 0 s . *s, 4 *$ 5 4," d ~i. .s 4 3 *I if 43 *4 l ll :s s 'S"'. 5 Ii" s* 48 .en. .10 *ro 13 12 ll* H* *11v 10* 12 l{j ' -Ill .!I 14 12 i3 12. *12-" II' 12 10. Jl:'. '119 67o**** *0 16 l6O 14, .*18 (l O*I:>. '(} 14 Q 17' IS 0 u () .. o. 15 {! 153* 6: o.' o. ..1s* IS 0 II!' 019. 110. o' o* .*IJ. .If IJ:.*19. .t;t.' 0: 14 16 . W4 o,1'111111 31 31* *31 3~ *32, *30 JJ , , '23* :l~ 29. 306' * .'flilut: .*3% .. Jl' 3~ 3G :;u36' *30: J4' *.Jo. 33. 331 10~'7%ilny .R '1li<*tcni1mtmr* ....... :U~nlicnl<>11mi1bor':... ... 't"olol 'P~l' To111t.I .L .J A ~. 6. 1 , f!.~ . 10 *)* l. .. 3 -..!: . ,5 6 .7 8 :9 ilO'.1 O* ..*Q*.o (l. *o .:. o,*. *il *O O* O* :if:: ;(I .. "I *O,i 0 .:o 11'*; O* O* 0 .. (Ji>"°' 0 *O.~fl* I) *o *O (l {I* (i'.: (l () 0 2 :o' .... o. .*o 0 0 "O .0 0 .. i) o. 0 J "(}" JV *o o. . 0*. o. -0 (); o. ,il <o: *3 o:;: *IJ' *u l.l*. *o I.I: l). O* . 0 'j)/' .'Oi + .. *5:.: 4* s: .:4 4 .4. .'S . :.. 3 *. s:. .."\' .. .:43 .. . *4 ..ii :s *:5: .. .o** .4 $ !L l\. '>(i 5. S:I... 'S '1 JO" H'* ti" A6 u** lfi 0 ii \l.Fi ..ff IS *iM1:?* . . .. t~~(j* o ... It: 10* :ll* CL JJ. Al 11* t:ll* *16 T7*: U* 'IL HSO:.. *::1154 . *s:.6'7*. iJ .l~' 1"" 10 ;n u* 'Ill* . JZ.: 1.2 ..o...: o:*is** IS'.* 19" *ua* . ::Jl .o .. *o, 11."* *:o , -0..,.,. l'1 "19 *:19" 1.:1.'.13*'.Oi111:1!?4 0 177. VI"1*~141 'll" 32." 32 . 3S d!IJ. *.33'. JS** *.w:*, 33' . *3't 31:? .ToldI 11 JS J6. ':31 .'JG' '3.'i J!I 31 *33 J6 35\1Z:i.4% CtiritroJ.:2.Diiy ltcrilleiaii! nuinboi* 'ro1111 ;Qtiy. Rent1rn10 numtt<rr ~ro*lil,,_l_ 3, 6 .7 . .8 9. s .6 1 ,ll . !1.o" J 5 IO I. ;?. J . *4 .101.1 CO'*' o* *u o. O*'. 0 0 0 *n* .U. I. ..IL . ~o 0 *11 0 *o" o. O* :ll ..*o, .o-.:r ;*o;- *O* 0 0 0 0 .0 ...o. ..u. ..'ff', 0 ,z, O.' ... o.... ll 0 ... o.* 1)* :o o* **o .!!'. (1.:'f *o. 0'.' 0 "Q;. 0. () ..0 ... ]), (I () () <l **o* "ii)': *u .o. 0 .o. n* 'll U* 11 .:o 4 .)' :.4 ;S ,4, *.s . '4 A. 4., ;S .. s:: *:.c_;*** 1* .1**' .., .it* $.*: .s. '.L . ,I, *39 '* .. .6 .... $ . J3' . JO.. .. I:? .*1z. **12: 11.: lit* 1:!' ; 'J4'.. -ll 44 ... .. 'U9:". 4 s 'I()' .13'. :.ir 5 .::u. :*n... .to >Hl :.:.12.: :.. fl>.'* ..11' . 114 , '.'(J. '..dk O'. *:o ~ 0 0 0' :o.. D '*.O***. .. g '(j .. O< *"O' *a Q* .. o* **-o,, u 0 O* "(}* I). .7 '.'14* * ..ts* IS IS.. IS'. J9 16* IS IS" .JS Hill. '/* :rn* 11*. *IS l\J M '1'4" :"17 H* 17 IS' 152T0111r ar ..*n 31.' ;3'4.". \1% *;l(f. .30' .,31, ,34 : ':;J(., 373 .. .. Tot~I* ; .:29\ !*33 ;:'31' ,. z9-, i"1L 111' . Ji. *'11 *3J Jlh . JO$*li11y *tto1!llforii iiwnblir' Ti)(ul *Udy . .:Jtif illtntc nu1nJJer* 'l'tiiul r *z ., J. 4:.,. di l'f* '7 ll* !!* 'Ill' I 2 :3 4 .. 5* IL 1 fl II 'JQ.* J 0 o:* !f .0 ,<J 0" 0 .lL ..IJ ...it 0: (fI 0 .Cl*.u: 0 . -0* () :.-.o . 0 0 . 0,a.,* . o ....()...0 .... 2.. i,IJ.' ;'0 .0 0 : 0 "U O:' 0 ':O' fl' ... 2 **:*"** (J' *o.*: II *o .0 !I. -0 0. ,...3 ... :'()* ..*o,. **IJ 'O *o*: *o *:o:' o** **o. ll *Q. *3 0 *;0* 11. O'.** u !), II '0.l .0 II:' 0.......... ;!*: .. 5 .A. s ..*~$'.'.' ... 6 .. 4 *S j. .S, 48 ... : . :'S"* '$* 4 ..:..iL 5 . ..!) .. ...4'. '*l'i"*i .Ai.. A. *.so 5 .. _ ll* *'Iii' '12" *n *Jo 'JJ' :12 ltt: 'fl' CJ f.. 120 s Ill'. *10" *.14 tt:t XL ...14:. n** ' '.111! '.12 "I:\;' i20 ...(!'o; '(}: 0. ...D'18. **1x. ':18 .*14*, tl lti.: .~!'.. .dt 15** >>lll.. 14'. 0 0 HS5 ' ..(}; u '.o* .il .{)rs: l(I. I'S 11*O 0.u '() *II-ts j)' rs* ~o -Tlillll ;r4; ,35* 35 'J'f.* .33 l.3'4' ' 1 .J2 ,*3)* ;,.11: ..JO* 333 7 llfnl . .'20" '.Ill. '1$.' >J3* ;)!I: ' ll ,.;;n .. . J1 . '38' 31;. "J6 ... *3.l * .34. i'75 345* TVA I Sequoyah Nuclear Plant, OutfaJI 101 Augusto2 ..09, 2016* Chronic' Whole :Effi~ent Toxi~ity Test (EPA~82l-.R~02*0i3, Method l002.0) Cer.lo1lupl111Jo 1'11bi1J Chrooie'Whole Efflt1entToxicity Test* . EPA-82J..R..oi.. 013, MetbOd 100~.o QutrOtf Con,trol.Verifle3ti<Ju ofData Entry, Calenhttions~,. and StatisticalAnalyses P1*11jel'!l*n11m~r,: _ _ _ _ _~_ _ _ _1..,,1.,..ii3_7 Cont~i1trntii11i .lfonli1::ltem1mbei'Sm'l;!Vlil . A,*erage rcpnuJ1111tlon Co~llrflcnior Pcr~nt ft!dnclioli fr11ni.. **a*rl*Jioo (o/.il *lllioliiil cunll'ills (cy,,): (%} (01Tspri11g11~malc) 1 l J. 4. .5 (j 1 s '!f 10 '(%) Control*i 3'i JI 3j, 34 32 30 31' 18 29 29 100 J0.6 .. 5.6 Not applk~blc 16.7-% 31 :32 32* 35 .30 33. 35 ~9 Jl 32 mo 32;2 6.0 .5;2 2}.4°/ti 31 32 ~2 34 :p, 36' *. 30 31 34 31 1ilil 32;3. 5,7 -5.6 42.8% 34 JS 3~ 31 33 34. Jl 32 37' j() iOO 33.3 6.3 "8;8 85.6% 32 .32. 34 3(; 34 3& :w 34 30 .Jl IOO ,JJ;i 6.4 -8.:z 100% 37 35 ~6 3l 36 35 38 37 j3 36 100 t!S.4 5.11 -15.7 C1i1tirol*-.2 29 33 .31 29 <3i 28 3:! JI 31 30 HID 30.;; 4.9.* Notilpplicabl~. Hl0% l11t11l1e 35 33 ~s 32 37 38 32 36 33 ~4 1110 34;5 6.0 *13.l Outrnll 1!ll: MSD= Mii1ii11u1i1 Si1!1iili¢unt Piffer~11cu, DmmeWs i\*tSD v11tuc: PMSP"" Pcrccnl Minhm111rSig1illicn111' DifferencePMSl>: 6.6 P,MSb*is n n111osur~onest prccisiOlk The PMSD is lite minimum pcrcenl dtfli!rC11co hetwcc1Hlu: co111rol*nnd trcn1111cnt* 1hnl-c1111 be deQhir~ statistic11lly*signilt~nt iii a whole ~1TI110111.1osicity test .. i111i1l1et l>il11neli'!i ~so vnlue: J.40.J l.owor PMS[> bound detcnµin~'<l.by USP.PA(I011' pereentfloj =13%. PMSD:. 4.G* Upper PMSD bmmd dclermined by USBPA {90ui percenlilo) =47%, Lowet.tmd t!ppcr PMSD'bmmds w1ire detenninud from tl1e*IOlh and 90th pcrci:ntili:i, rospeclivcly. orPMSD datn from EPA's WET lnlerlnboratory V11rlilbilily Study (USt:!PAilOOln; USEPA, 200lb), USEP/\. 2001q, 200tb. Final Report: lntcrlnboratmy Variability Sht' ol'EPA Short-term Chronic and Acute Whole Effl11e111 Toi:icity Test Methods. Volumes I and 2*Appcndix. EpA,821.B-01*004 and EPA*821-IJ--Oh005. US cnvironmuntul l'rolliclio11 Agllocy, Cinch111ali. OH. TVA I Sequoyah Nuclear .Plaut, QutaU HU A-.gust 02-09, 2016 *Statistieal Analyses.* Cedodapl!nie Survival *andReprol:locUcm *Tast*Reprodocllon Start Dale:. 8!212016 Ti!sl'IO: CQFRCR . . Sample ID:. TVA/ SON, Outfaft 101End Dale: ai91201.S Sample Date: August *2016' 1.ab '°' .i;ts'Ett\llr, ~stlj,,g*$.ol.~i'oto¢ot f'WcHR"EPA-621-~:'02-013 Tj!S.tj3peci~ .sai~i:ileiype: oiviR,.Discbl!rse IVlonl!ol:int! Re?or( CQ..p!lrieid~ph!Jia dtib.la . comments:*Cone*%. 1' 2 3 .. *4: :5: 6' 7 a. s '10 Conlrol-1 *31.ooo* 31;000*31:000 34;1JOO 32.000 30,000* 3Ul00. '28;000' ~;OW 29.000 Control-2 29.000 33.000 *. 3'1.000 29.000 31.~~tl 28.000 32.000: '31,000 '31'000 30,000 10.7 a1.ooo* 32.000 " 32;000 35:ooD 3MQO 33.000 *as.ooo. *29,000 ~(iop* *3ZODO 2i.4 31,000 32.000: , 32.ooo 34.00Q *32.QOO 36.000 30,000. *:n.0,00 3,4':..000 ~toou 42.8 34.ooo 35,000, l 35.000* 31;oob 33.000 34.000 .32.000 *32~000 37:ooct .3().()Clti 85.6 .32.000 32'000 : 34;000 3G.OOO 34.noo a&;OOO 3p.OOQ 34--9(10 3q;Oot> aacoo 100 37.00() 35.000 .. $.000 a~.ooo ~EtOOO 35.000 3BJ:l00 37.000,. 33:00()' 36.J)OO*inlake 35.000 33.~oo . 3S:OOO 32.00.0 3('.~000 3a-Ooo *az:oou ,36;00Cl. siooo* adgo* Transfoi'm:'Untr.aru;formed* 1*Tailed' *rsoto.nic Cone"% Contr01*1 Mean. '30;000 N*Mean .Moail.1-0033 *-so,soo.* 2s.ooo Min Max' 3;;.;000*C\1%. 5.597-N. 10 . t-'Stat* ...Critic::aj MSD Milan * ~Mean'. 32~17 1.COOI}' Contto1:2. 10.l' 30.500 32~200 1*.0000 30J~O() 2!t0:DD 1.0557* 32;20.0 29~000 ;;:~gg 4~948 G.00110 10 -1.81G. 2.281 2;014 32.817 MOOD 21,4 32.300 1.0590 3~00 30.0,D(i **3MOO s:ssz. 10 ~.,,s~o ?;281' :zo14 3Z.S17 1.0000 42:8: 33.300 1.0918: 33;300* 3tt0 37.000 6.339 10 .3;oes 2:-287* 2.0.14 32.81'1 1.0000 85.6 33.100 1,0652 33.100* 30.000 36.ooo 6.446 10 22.s3S :<:281 2~0.14 3~.817 ~.ooo<r 100 35.400 l.1607 .. .35,400 3,.ooii 3a.ooo 5.BSS 10 .5.449 ~7 .2.!)14 32:817 1,00@lntake 3.4.500 1.1311 ~4.590 .32.000 38.000 5.99.S 10 Auxiliary Tasts 'Critk:al SkM.Y"' ....*Kurt ..Kolmogorov o T'*!I i(ldicates normal dislribtJl!on* (p*> 0.01} 1,035 Bartletfs TesUndlcates equal variances '(p = o.99} 15.08&3' The. control means aw not st nllican . dllferer;t . -.*o.89TIJ O!lnnett'sTest * '100 >100 Trealmentsvs.control-1:Lin*oa.t 11\terp.olaticon .(20!) Resa~plos} Point *% *so ss% ct:*. *s1tow ICOS "'100 1c10 >100. 1c15 >100 1.c ..--------------. IC20 ::>1QO *0.9 IC25 >10i:>* o.a IC40 >10.0 IW .IC50 >iOO. *D.Eio:s' gMo~~ o.z op-e.-.D-C..,_...,,. 0,1 ____ ~t) *;:*.: ""'\~: . .,.,. .... * - "":*~~0.2.o.~-.-. ........................,.......-........_.,.~...,.j p 40 35 30 g25 -e= 20 Q. ~ 15 tO 5o. ....~c0~. .~"'-* ~ a:i N ~ 0 e ""g,S~ 0 .u Page44 of90 TVA_ I Sequ~yah :Nudear Plant~ Outfall 101, - Intake August. 02..09, *2016 Statistical Analyses . Ceriodaphnia survival and Re:pro_duction Test-Reproduction Start:Date< 812120'16 Test IQ: Cc!F~CR -- Sample m: iVA I SQN Outfall 101 ~Intake End Date: aie1201'6: *l:,kibJD:_ ETS*E'nvir. Testing Sol. Safriple Type: D!\l'fR-Oischarge Monitoring Report S~l'fiple bate; Acigust 201~ Protocol: fWCHR-EPA-821:..R"02-0.13 Test Species: CO-Ceriodaphrria dUbia ..Comments: . *Cone-% 1 2: -a_ 4. 5 s* .1 8 9 10 Corttro(:.t '31.000 31.0QO 31,QOP 34.000 32;000 30.000 3'LOOO. 28~000* 29.000 2.9.000.control'-'2 29:000 a3:ooo :;1;oeo* :29.000 31;000. 28;!)00 s:rnoo 31.:000 31.000 30.0C)O . 10,7 ~1;000 ~2.00i:i' 32;oop :35;000 .39,000_* as.ooo 35.000 .29.ooo 33~000 32;00021 4 31;000- *32.-000 32;00() 34;000 a2,uoo as.coo- ao,ooo 31,000 34.000 31.!lOO 42-.8 34.,000 35.000 35.00Q- 31.aoo 33,000 34.QOO 3;2,00Q 32;0(}0 a7-.ooo 30.000 8'5..6 :32.000 a2:doo $4.000 36.000 34,(lCio 3$.ooo 30~000 34.000 3o:ooo 33.000 10()" '37.oOO a~tooo~;ggg* 31.0PO as.ooo 35,0QO aiiooo 3_7.000 33,000 36.ooo.(ntake *35;000 33.00ci 32".000 37.ooO. 38',()00 32.ooo J6,ol)O 33.000 34,000 1;;Talled Co.nc-% N_ -t"8tat :Critfoal - MSO Corttrol-1 30;s_oo 1.ooa? * - so.600 - 21rooo* - 34:000 5;597 10* contror-2 a0;soo: t;oooo so,500_- '~a.coo 33~boo 4.~48 10 10.7 s2.2oo 1.os57* a2.200 z~:ooo 35.ooo aoo1 10 21.4 s2.aoo 1';0$90 :3_2.30o 30.000 3s:ooo 5.s62 10 42;8 ~3.~o9 1,091a 33.3Q9 *3Q,ooo s1~000 6.339 10 .aa6 33..100 1-0852 -33100 30.. 000 36,000 6.440 10 10ll 35.-4bo 1*:1607 as:4oo 31,,000 38~00() 5,835 10 f~take. ~.so& 1.1311 34~$00 32J3oo. JfLOOO s.995 10 -4:94Q ~ 1 ;734 - 1'404 Auxiliary Tests; - ** :StatiStic _.* *------ _Critieal _ .Skew Kurt Shaplro*Wilk'S~t. !ndieate$hQtmal.distrib-UVqn (P > 0;01) 0~9388 . 0;868 0.22443 -0.7205 F:.Test indfo?~ ¢qu¢J*)l~riant:es {p =();36)' . 1:87805 6~54109 Tne. control tnE!ao~ are not sisnlficanflY.aii'fereot'cp_-= 0:89). 0.13853 -<.10092 MSDu MSOp M$B MSE F-Prob df H?moscedast1¢'.t Test_'iridj~ M-$ignlficant differences 1.49401' 0:.04603 80 3'27778 1, 1E~04 1, 18 . treatmentS vs ContrbH2 - Dose-Response Plot 1-tail, o:os level of sigriifii::ance .2'.25_- u -_~20.-* Q; -~ 15. - 10". 5 Q~~-,----N~.----~~,__----~~.--,.....,..~=~.~--~~.----~0.-'----f~ -~ g* ~* ;* t: : e s ,g 5 J; o* o-Page45 of90 I ; ~' j~)f~~: Page~o..f7 I Specfes: Ci!riodap/inia,dubi~ Qate:.,os4u.:16 Client: TVA I Sequoyah .Nu~lear Plant. Outfall 101. Daill Chemis :* ** 0 Concentration I CONTROL I I 10.7% I 2L4.% 'l 42.8% 85.6% I r IOO,% 100% *Alkalinity *i Intake ( *CaCQYL)', -'rHardness "':L:(mo CaCO;/L) ,,~ "'TR chl!)rine (mg/L).Tem erature C°C}.ot "'Temperatures p¢qtmed atthe time of test *i!litiatioo. reiiew~ temtinati()nby the anatyst-idetitifiediR~e Daily Renewat ' !nf(lnnation table!~ on Page 1. pJbliitity, hlirdnerui.arui:tqtal residual-~hlorine performed by,~analyst:identili'ett op;t!JeJieqph** sheet specific foreadunalysls 1111d '~bed!O- thili bench sheet by: :'/A . age46 of90 \ 111* iP 5::::....r_,.,,.._,.._; Page7 of7 Date: 08-02-16 Coneen.. 'Parameter tration CONTROL llA% 42.8% 85.6% 100% 100% Intakeffar$Incis .<m. CaCO.;/LJTR cblorine tmgtL:JTem ernture {"C)TumjJeratures pcrfonned nt tlietiine oftest initiation, renewal or termination by the analy.;tidcntified .in !he Daily Renewal ln~tmati0.J1 tllble.** 'ocatedon i;age .L ~a~iriity.* ha~esis and: total tesid:'J\lori)1~ perfof!l'led by tlie*analyst identified on the bench.slJeet sPectfic :for each analysJS and trans~nbed tlHliis bench sheet by:. . , age47 of90 SOPATI l -Exhibit ATI l 2, revision I 1-0H4 TVA I Sequoyah Nuclear Plant, Outfall 101, Non-treated August 02-09, 2016 Ceriodap/mia tlltbia Chronic Whole Effluent Toxicity Test EPA-82l-l{-Q2-0t3, Method 1002.0 Pally ChemiCnl Analyses 1*roj11~t.11umbcr:*_*_______1_16_3_7 Concentration Parnmctcr D11vo Dn'.l'J. Dn~*a 011rl D11y4 t>nv*6,(l!ltlUI .* Fi1111l 111m111 Flmtl ** lnllinl FinallnltlBI lnltll\1. Finni ll1lllnl Flmll fulllal Fin11I oU<Sm . 8.09 7.92 7.88 .7;86 1;80 7:89 .7.83 . 8i03 7.99 i.92 7.73 1.97 7.94 7.99. 7.8. 7.8 .7.8. 7.8 ?JI 7.11 7:8 8.0 7.8 8.0 7.8 7.8 308 *.*... * ;...-. ;. *Jlll :'*,. ' ..... 318 ..*. 317 ...... CoiidlictMtv tumhoi/cm).. *.. *.:: .... ~*.*~* . "59 ,. .,. ~-.: -~- ';i..,~*-**~ *~-as *.- . ,., .*._.,. ** * .. **.. **~~ i 90 *:*.::.:**'."' . :..,; --**-** - .... y*~***** , *..*..Tem111:ratttl'll'l"C\ :14:1 2s.o24.1 :!4~7 .. 24.7 . 25;0.nu1sm. ... *s.06 . 7.91 7.9S . 7,SS . 7i:l4 7.89 ,8;02 .. ..8;12 MO 7,91 7,97 8.10.. "91 DO trnulf .\. ..... 7)1 7.8 *1.9 7.9' 7.8 .Al.O 7.9. 8;0 s:o .* 1.9 -1.9 16.7% . **309 *.******** ....Cond11rtMlv litml1os/cml :20s '*****-~*:*,,._,.-*,: .29S 306 24:8 .. .25;0 24.8nll CSlll 7.94 7,95 7.90 ;7;.89 8.01 8.10 7.90 1.92 ..DOfn111/Ll *1.9 :* 7:8 7;9 7.9 8.0 ,. 7,9. 7.9 . Coitducilvltv tumhosfcm\ 297 "'* ...... *;. '.;283 :... 296 301 11u.csm .s;o3 . 7.93 7.9S . 7.86 .7.90 7.89 7.96 8.03 .. 8.09 . Ml 7.94 8.03 7.93 DO line.IL\ J.9 *7;8 7;9 7.9. ';7.9 8.0 s;o 8.0 8:0 1;!1 1.9 , Comluctlvltv l11mhosfcim\ 263 ..................., 24.8 .8,QI * *. 7.97 7.91 . 7.89 7,96 . 8.04ss:cw.. DOtinl!JLl* s:o ito 7.9 s.o *s.o s.o CtiniludMlv,lumbo!lcml -228 ;'.' *; *...:.:f'::.......*.. 11UtSm 3,01 ..7;97 . 7.98 .. 7.90 *1.92 wo. s;oo * *:s.os8;1 I " 7;93 .7.97 :~;02 . 8.1l6 ?;96 1:8 7.9 8.0. *7,9: 8;0 7J) * *8.0 ' SJ Cotiductlvliv tumhoslcm\Alkilllrilty htil!lb CnCO>l Total ltcisidual . Cllfol"llie lm!!IL) <O~lO '.: *~.-> . ' . , .. ;-;;';'.2$.0 24.7 !?*k9 24.8 24JI lS,2 . 2'-0 ." 25.0 ' 14~9. 2$;1) %4;!) ~d 24.~ 24.9 nUldJI 7,96 7.96 .8.00 7,92 . 1.93 us. 7.99 .8-08 Jl.IO 7M 7.98 8.03 8.06 7.98 8;0 7.9 . *'8,0 ....... 7;9 8.0 ... ' 7.9.' -8./J '* ' 8.2 8;2 8.l *tkl 7.9 ... 8.l 8.0 199 ,, .: .;':~305 , :;=-~;: :.:*::*:-:*,:** ,211 '"'.,, Alkalinity hng/L CtlC01)- 'roliil IteslCli1111* Clilorl.1i~ tml!ltl ., I . 'l~l (i.i ~ \ 'Fu~ $Qn101~080216Cl1e . i11XJ*Entered by: J, SumReviewed by:+Page 2( Page_}__ of_/_ Total Residual Chlodne (OJnON-1977) Matrix: Wat¢r, RL"'0,10 mglL ' Meter: Accumet Model AR25pfUion Meter .Arialj!St l~ .f}fp . ,. Iodide reagent ,1---'/_N_JZ..,,..]=_.__S_"l>_ _-t Dare*am~lyzedif :-.oz.::/~ . Acid reagent: ....._J_'.'fi}_Q_f-_4_')_~ CalihtaiUm';. ..uo.mglt NQtq.* ;f"ot:s~ples 'Wlifr a ~!dual chlorine bf ? LQ mg!(,, the samples miistbe;diluted lt> be withfotbe ealibmtion range.*Laburatorv controista11ilanl: 'True value (TV) Measiir value' (J,\'IY} %RS='MY /TVx 100 number . ,(mg/L). *(mg/L) . (acceptable range=.9D to 111)%} 0.50 Sample Sampie: cha'ractedsncs * .Residual chlorine * %RPD = f(S -D) /[(S+D)/2 x 100 number (m IL) , .. *acceptable range =::: 10%) s,to..o~?I>>£ .o~oz~:L-:~,,....._-----~sam;ple Sample ID Sample cliara~teristies Residual chlorine number, (m .L) LO-oo/i/r Referc~ce standard Tru~ vnlue (TV) Measured vali.le (MV) " % RS::;:- MV /TV x 100 n.umbet . :(mg/l:,) (mglL) (aeeeptable rn.nge =90 t1d H)%) Reviewed by / (ft_=-Cfl:-'*~Q'l.=-'*-1=~====='.Dote* reviewed ._t-_-__ Page49 of90 SOP C8'-Exhlbit CS. I, revision OI-01-13~~* lf Pag~ _'l_* ~f-~_I I I T:C),tal 'J.tcsidJ{~tChl~rin~(O~ON.,i97~'Matti!<: Wa$7, R:L =*0,10 mglt ,Meter. A~u)]l(lt M9del A.R.25 pHflon 'Meter I Analyst ING *.*Date analyzedo6-o~-I~ Calibratitm: 1*::::: 11:m I I Referenc:C.sta~thlrd * ; *tr:ue.v:JJµ_e(;f\I} * % ffSi=.M\t;ti'VxilM number {mg(LJ.ta~~pt:ibtc r:a~e-"'*9ffio; .t~O%)recisioi1: . Sample Sample IQ .* *saniple chara'~terntic~ 'Re5iduaH:hlotitui I number. m ft. . s.jjp.,£170'D C'q~j;~Jt) * *-*-----""_._..,..mD I Soni le measurements:Sample I number S'ampfoID I I I I I Reference standard True. val~e (l'V) Me.asurid value* :(1\-'lYl'° *%R'S'= i\:lY'tn.r,i 1~ I number (IJ1g/L) * (ing/L) ... * *fa~epti$Je.tnug,c=:pa:fo*lill0A>l '* I *Reviewed'~y Date reviewed; r .. *. ,j~tr. I(,* . .. I Page50of90 Page. 28' Pag~_{_of _L_*Tqt~l Re~id~~~ CJ;J1ur~ f0Rt(JN.:t977lMatrix: W~ter. Rt =l:U otng/L llile=:li--a-1E .J Meter. Accumet ModeLAlU~ pH/Ion Meter ** *::: 1~~-.~...,..;~-~.,.,...**47J~.~----.Note; Forsamp~'Witha ~~dual cbl~e of > L(hi~4 ihe samp!$.11iust bci di!~ tt> be within tile calibratio1J i'angc,. . ' . .. . ' -~ .l);u~:'.Value (tv) *Meas11red'valu.e (l\IV)'(izjglL) ' . (ffi,g(L). S:u:nple*.**number Sample cha:racteristks N'i11e: AllSilm,~les.'\".!!rrNliml)'zei:J'in*ex~ofEPAremml!itCled'hcildin1J1i1 . .La/JoiiitO. *. .*c0ifli4J:startdat'lf:.. *~rmn~standard ~~~,vaJUe (TV)*l\f~iir~' ¥:du¢* '(MVl 3 RS= MV I TV x*lOO***nutilblir: *. tn'ij:/i:;), . ' (lngtC.l * {!ltCcptab!e range= 90 to HO~)Reviewed b_}r 11-._ __,M------1 Datereviewed .....-~*~IP~.._,'l... fk..._._,Jli:.=-*_ __, Page51 of90 SOP C8-ExhibitC8.l 1 revision 01-01-13 Pa~ ~l I I I Piige; I *Of v Dateanalyzed v-Analyst' 'I......-'.00---..-w-......,~-.- ' " " I*I.*..*Titram i1omwlitv.and:mu/U11/iet-tfetermb1atin11:. pH of l'ltmnality * *1* * *Norina~t)>*(j\t) of~iS:Q4 , pH F,:i~r*orM~l(ipl~er Deionized T:itrant -.:h~ Q~n. *' 'Eiid Total. =($'-miNaiC03 x o,~s)i:E' =(Nli. $JOOQ)IIO() l1lisan1ple wa~ . reference . s(a~()al'.d .mt : ml ml c= 0~251.t . * * ::N;c,.'.so.o=4.SS.U. number number <El*. *(ncJ:cptable~nge.;..,l)J)ts~*~2ii)Referen~ standard *True vl.\fu~ *Safllple ::.Atki\liniw**@IY) . %~.=MV'iTVx 100 number (TV) ** yqJttme Begfu : End Tow.*; M1dtipner* .. ~(~~.C~Ll** (11.ccepta:blc.nmgc {mgCacOJi'M cin1. .ml ;ml nil '. . * * * ::"'!10 tQ:tt!);.OAif H)O .. ** *... -*,\\:ll(li6riity .. . $RPD"=SampleTotal . Miil.tipli~ * (iilgOiC.Q#Q. * * {($'~*:D)fl{S.fD).t2J}x.1op number *ml . . <*'11>l;abie range =.;l; 10%}~.l . * .* t{)C), . ;S Duplicate (Bl . .J Matrlt soike reco11e,.Y:Reference $ndard Spikla v11.1µ~. Sa111ple number . '(SV) volume ,' Beg!n*'ml *ErW: . Totili ;nii. i *~*** "(mi!:CaCO.JL) Jml}S~mple alkaf!nifY (B} .M~asured ~pi!¢ \jat"ue (~VJ* * %':a=:MV /SV,x:lOQ.(mi. ~co~L) i\'I:V,,;;;A. ... :i,r . . -{ai:~pt.dlt~ range (me. CllCQVL} . =-1Sto:il5"4')Samole mtas11rf!l11e11/s: ISample;votum~ Begin ~~d ' l'crtaJ AUcanilltY I._J Sample number . fin.I} inf . , m1* . ml * * .Muiti1>1ier (me CnC0.1/L) ru* .., U*J ,(po* .* ..... $:ia JPage52of90 J o.: Page ~2..0.:1 ~- 2.S s=~~~J;:::L Pa.,,ae . of_V..___l A.lkalinity. (SM' 2320 }J;..J.997)*1 . A~tl **LG, ~ .~at)'ted** OO~P'O~ni *.f* Mattix: W.atet. Rt= C'9 mgCaCQiL Titrate*saJ11ples to Time initiated ,........_ uQO..llllts Time completed . """"""--1 pll = 4~s s~u. . TitraniutJtm.atiiy 11it.d:11~ultfpiier det~11i1i1itfim~:Normilify :Normality,'(N) o~;~so.r pH Factor ot Multiplier 1 =4;ss,u. ri *number_,..fererice .l Mmber: heck stan~~id 1 1.I. Total*:*. mf . .* 1 -mi* "::i{S ml'Na1CO;J x\U.1)5)/E' * =o~sm**. *abic ri*=i'o:Q.1so ~o;~) ..(N~*50000)/ 100 ml sample* --iV.x.500 ~ -*1 *-~---,:.....-*.-..:-Ui.b.t1ril,tofY°CQi1tt..Ol sltfi;tlti.td: 1l*ter~::::~nr*1. ~rtr~tu~. ',* . ~~: *.. *.(gig ~COJ/t;)_. , (m.l)I B~g~~ En~ J** Ti>~t *j Mtdtiptter** (~CaCC>.1'L) ml . mt , ml"**i *Alkaliaity (MV) %RS=MV /TVx 100(:u:ccptable range=90to 110%) ~;NSS l~*U\;L. ] . 10tl .t 100 :~~~'3:-fHilF:q. 8 I lO:<f *1 qs qe.01, tI 'i!i:i~oit: Sample-~1\!i A.~alinity. %RPD= .$ampte:in * -volume J. B~n *.1 :End 1 *Total MiiH,ipliet I (mg CaC03lL) ((S -D) /[(S+D)/21} x J 00 j *(ml) .... ml * *ml . mr (aeccptablc range=::.10%) . .IPO I*}t'o;o 1111:1o, !Jo.~ J 1.s Uu.i .J; J \~\# J.21.. l Uo*S:: "* fD 100...,.,,~. *.11...: ,.#.~ .*l~-iuir..,,,sp~..* ..._.. , ...;,,.ove"'** .* *..r** *. Refere~~.Sta~°nrd ,. .Sp~.~ vain~ ' Sl:tmJ>le* Spike alkalitiity (A) l ntlmber * (SV) *<rim' cacil31.L> , :(l'll1) .1/oliun~ Jl~n End. Tot.al'j-ml. ...ml , . ml*I j\IJilltiplier * {mg CaCOJil..) lJ4~Sl~lY 50 \t:Q l\\"" I~;'&I! ISSJ ;fo:.o ~ lSb l *samtJle*lkalu:iitr(B) . ** (mg c~~OJ!L> * * * . %'R=*MVISV:x: lOrJ. . (lll:!cepilibf¢: range . l !)°() . =7Stol25%)J 100.w. Snmoie m~l1S11rl!l)te11ts: .~ ........*.Sample .votµ-me Begiti.* , En.d I. Total .Alkalinity -Sample number- :Sainole ID . * (mi)' ..* -ml . *mL .. ml.* Multiplier (ing CaCOJIL)n~t;tt.?A~-.,.* ..L*ebJ .. *:.oo ,;t,.tt 1.$l1 I G.;10;{).,,.;;;,J~:'****. . ... *.. . . . . > ** , qq ""'**It*J . l O~.!ll~U"* P.>.,*Jo: n1~,.<S) J. tu~ 'J -26' .3<;:~ 11415:01 ~ti\o~µ 'I 1-J] i.11i~~cu 280 l\rM"~*:"i.~:.J~lfp. lhb: 0()\ .ffij. 'l..1 I I ..:a.a ,,-., . . . ---- .... 1r1"\J i~ti.t<Ps-~"\.l . l . !fm:tl\)T I J'J.~*t .... -.f, JJ,ti. *J.1s ~l. . -*"Li ..~1* 1 ~g: \!~'!I- *"(JJ ro ,It. bl~*"Li * ;_ .1*rrll 60\_ .* .J-""**1: J."**.... -* .1;t.t)*,,, ...c~WJ , . LJ.J40.. . :"I .W q-z J .lb. . t)g:Qb...... .. ~*!'.-\I..,.. *1** I' . . 1\IM.~.(J~ :'l~t1Mil;tt0£l.J... .L i'i . :j <ft rm1N" ..io.ol:tasJ a.s.'25.tP. l~S,I l:q, -=ro I' 313 25 7'-1*. '"--..(",, "l1l:i~ :1* l f J1£ l\o<5l-O\~G\ l'-rW6A l 100 . . ."::> .' '°1' ~ l' '-IJ RevieWed by: I ~ .' f Date reviewed: I o& *t>\.*1 b Page 53 of90_.J* ~OP C6 - Exhibit C6.l, revision 01 I3,p~~ ltJ ~Pag&. a or. 'V,Afkalbiity (Sl\1::2340'".IJ~l991)Matrix: Water:~ lU, ~ l,-0 D18 Cf:!C03'1;;.Ttttat* sam)>~ *~ ,.:;;~,411i~ f'~pH= 4S s.:u. Ti1rm1t llormtilitv a11d n111lti1Jlierileier1niliatiort:' pH of *. Normality. I.:. *f9tii#t1it;y (JVtof'li'iSQ4 :P.¥ JfActot. or :M:!lltiplier Deionized water Titrant re1e...~-check'~rd .' Begin .~:End:*ml ' ! Tl)~l 'ml*,=ts. . mUii,!hCOs_(JjS/jli~:OilJ$)~: =(N'X :.soooo)l 1on*m1 sa~pte.* .. '""'JVx,50(}4.5 s~u. . number . namber*' *'*able J'-iing~.;, fl~Ol~O ~ 0:1!220}: .Lnboratorv eo11trol stamlard: . Reference stru1dani' 1'.ruevaiui sa,rople.* . i A!kalin)iy,(M\l.l, ' %'RS~MV/Tv ,;<JOO number (TV) voiume Begin .End , T9tAI, ~uJtlpJi~J" ,(mg-'CaCO$Ij) * .(~i:ceptallte 111~* (mg ca~().s/L) (ml) n11 1111* 1111 * * * =!J~.10.IJPo~>* *iOO JOU'Sataple .Alkalil!it;y %RPD=* ; v9lt11n¢ .* .~.~:~ : *.*.'.Em:~,d.* * , Total '!Mu~piier (mg<f\.C.OJ.14 . * ((S~D)Jf(S+DJ/Zl}xl'.(ml). .... tiil :(~ci:~iablecan,ge,.,,::1:;ro%)*:r a.~.S" *93ef . . . iA.o* ... ** o, Duplieate (B)'Mauixsoikerecov.erw Reference standard : Spike val11.e ~mpfe number (S\I) ***volume : Begi!'I , EndTotal J\lful(ipller (me CaC03/L) lini) . iiJI . mf .'. , ml Sampi,e alkillin~ty. (~)* * *** l\fe~ured spif<J.1.VafiJ~.~~ * %iR=::.MYfSVx100.(!U~,CaCQ¥J:,) . MV=*A,,,B . (accept~bilth.R&c , {ingCaCO,ilb) *. '.=;7ftt>'I.~) .*.,....'O\ .~.*:.;' .I U\..I'~'-- ~Smnple 11ieasure111e11ts: 8aJJ1ple vp1t1me Begin. * :En1:l To~i. Ait<aUnity* Samole number SamolelD fmti .. .J ml' 11t1, *mlMulfinlier lmg CaCi>.J/L>, .\f.l,"j J .J J Page 54 of90*'j:~"' ~lf)'-i l .Page '-l Page of lo l { .Alkalinity (SM.232Q :B~f99:7)M!itrix: Water, RL= I.OmgCaC~/L ITitrate sam pies to pM=*4:5S.U~, '* . _Tbne initiated Time completed ...__ _____UPo\\I"'ill' __,Titrantrzarlitaliiv *llf1d i11t1Jti11ifer ife1em1iltiitfon: . pH of: *N'ormalityNor.m~Jify (N) of<l:liS0.1 pH Facror or Multiplier Defonizecl * Titrant 'Clteck; 'IJegin End * ,'to,t.11 =:(S:mlNa:iC03 x Q.OS)IE = (Nx 50000)/ )()0 ml sample'\ &Le, : -* *nee stitndard , ml, m* m'i *tl:.25/E Nx500 4$S*U~ , . nu'niber *: numoer m'i .. , tai:teptaJ:iie range>= ~)lt$o ~ 0.0220)LbIt: orli(orl~1ifto *$fan 'ttii : Refet~,n~e;standard .* Truevabie. ~::unple Alkalinity (MV) % RS=MV /TV x 100 number . "{~V) . *. volume 'Begin '*End Total ~ultip)ier (mg C'lCOJIL) (acceptable range'(mg.CaCO,Vl,) . <<mn .ml ml ' ml =90 to llll%) \ S~l~\4. J;90 lOQ .leA 'Z.e.e ct.ii:\ toLo q~ qq,b/S.rimpleAlkalinity %RPD=volume Begin . *End Tntat * ' Multiplier {mg CaCO.YL) {(S ~ D) /{(S+D)/2)} x IOI} t~tl ml ml ml (acccptnblc range =+/- 10%) JJ:sZ Matri-c sJJike t¢e,overy:' R.efenm~'stam:taril ' *Spik~ v~llle . S~mple Spike alkalinicy (A) . number . {S\r) vriiume Begin End Total M*uitiplier *: (mg CaCO.,/L) * (iugCaC03'Ul (nil) nil mJ ..ml smnp~~a1kaJin'ify CBJ 1\1eas11red spike vahie*(MV) % R=MV1SV:x100 *'(m~ Oitoa!LJ lWV'=A-8 . (acceptable range(.m1t CaCOJfLl =75 toi25%) tD0.0).' Sample volume ~egiit End Total Alk:tlinity Sam pre number : Samnle lD I . (tri}) m1. nd' ml. Multinlier (m!! CaCOJiL) )OO . . I o:o JY*lil- 2.1.sS:* J I Tl"'t~.o IBo Page55of90 SOP C6 - Exhibit C6. l, revision 01-0 l-13*?.'\' ,t>age_.-~*l'a$e . . 5 of l a Analyst Date anatyted I:a .I t. 'i\Q. \p. _Titra11t 11ormalitV a11d 11mltwlie1'<det.enniiu1tioit:, pH of Nonnapty N.orrnqlity'.(M.'Oi'~¢:4 pH Fl(tfoi' pr Mll)Uplier . Deionized, Titrant ._....,..~ = (S ml N'a2GOs~. ~.OS)IE:. , =@.l5tt. . * *;;::*ii\t':X:~OOQO)/lUOmlsample . . ~Nx -soo*, . . .water reference s~dard 4-5 S.U. number number ble-t;1.?o~t~IJ-M~OI~-** .. *-~{(;Laboratory eonti'ol stmufani: Referenc¢ standftl:'tJ : Truevalue * ~mple Aikaiiitify (MVJ %~ =<,~/ l'V :X 100 number ' (TV) , volume:* lfe.gJn*.... "* . End l Tohil (ll'!g.¢ilt<WLl ** (aeeeptabl~l'!lngi:(mg Ca(;Q.VL} ; lml) ml ml' ml * * =~O:toJt((%) Matrix snike reco~*ery: References~ndard *Spilfo;value. 'Sample= ~i>i@attqilmity (Al number * {SV) volume . iif;!gin . End.. *. total, .Multlp)ier ! * (nj~, t':lg()JJL)**{m~.CaCbJIL) . *(Din Oil . ml ml* . *t6Q: *. !>!iinp~:a1kaiintty;(~) '*= Measure~f$1Jike; valn~ (~ *%,R~~VJ*SV.'x10!l (mg~ Oi003'L) , . ~=A"'~ . (accep~abft.'rijilgc' (me caoouty . . "';' 75:19125%) . -f'Q ADY' .Sam le meos11remei1ts: Reviewed by* y ** * . 'r *&c* .'f .!)ate.re~~ew~; t .bi* 6~~\'° .1 Page 56 of90 SOl?;Cp.'- ~xil,bit C~;;Ji*:Ie"'.i$i~n .Q.hOl ~nl ..,(*~;Page (.p Page of"'3£g Lo l ).~~ri~:;~Alkalinity ($1\1] 23ZO B.;1997) l 'MatriX: Water~. :llL = l,-O mg CaCO;/L. .Time<initiated t :..............<Je01lt Titrate._s~Rl:ples* to* Time completed ~1 pH=4.Ss.u~...'U ;..1-Notjtitditjr ; Ntn'malify (IV) ufH1SO-t pH Factor or Multiplier l *, bclbn.tea*** Titntrn;' ,y#.ter * . r~feten~ -"::<4;;5,S;UL : .<1ttimbet *. *- * .* . : ,* *:Begin ; ~d : Totaf * . .$fandiirf$.r number ID* *...:::iL . ml** * {Ef : ;;:_ (5: nitNii:CQ.l xitQS)fE =IJ~iS/E .. &ter..ang~".""o.01so:-~o) * =(Nx.50000)/ .100 ml sam1>le =Nx500 'l Refer~ce sta11ifard l'tu1' value Sample . * . Alkalinity (MV} %RS=MV /TVx 100 , ,(mg.~~~~ volume Ji:nd *Ti!tal ** Multiplier;: (ing CaCOvL)Begin '(acceptable range numb.er ;rtnl}, ml ml ' mr '" =90to 110%) Silmple Alkalinity %RPD=-Si,tb!plcffD. -volume :Begin-*.' £n:d*Total Multiplier (mgc.1co,/L) {(S - D) /[(S+D)/2]} x I 00<mn . ' rnl *' ml ml ' (acceptable range= ::1: 10%)References~ndatd :Spike value ~ample ' Spike alkalinity (A) n~ber (SV) * . voiume D~ln - E~d Tctnl :Mt!:ltiplier '. . (mg CnCO.i/L)imreOiCO.,/tt '(mil 'ml. * . *ml * *ml$ample a1k1!Jiiiity:(B)M~surecl spike vnl~!i!-(l\'IV) . % R= l\iIVISV'x IOO'(m~ cBCOJJL}. MV=A-B (acccj>tablc range I f. CaCOalL . = 75 t~ U5%J .J ~ : .OJ._J Samprevolu1r1e Begin* End* TDt81 Alkalin'ty tmnml . .ml mi Mtdtiulier lin~ CnCO:i/L}no J 3000 l J ZCXJO ?.1-v"JQ J "2..tbO JDate reviewed: I t>&*b\ii"l!o Page57of90, SOP C6- Exhibit C6.l. revision O1-01-13..1 0 '!l r i" .,r--;1~'. "'~i?l s i * -1_____i--' *-,~. _./Page ' . Gf' -_j Ji:~dness (SM 2346C.-U97) l Analyst I ~ '. I RI,,==* J,O.mg C~OOJL . Time~ initiated: *1*.ti.:z.f.e. . ____,_I Date analyzed .te~Qe.!l/1,, * . **__~_.~l Time complete<t . . .:_.ty..:;*f Tiirant 11<Jm1alftj1 imd nmlifpiieiileremJii1tlti01i:. TUra.nt.J;onn***lllity cl.. 1eckile¢in. 11 En~ *1 T-0ta.*.t 'Norm::ility {/Y} 9f EQTA pl:t'factor or Multipiier l . ..0:0~21.E =QV~ $.t)OQ0)/50 :pd sampie 1* 'refertWte s~ndard . ml : ml ml number . .. HI number . . * . (E). *{acc:cptab1c-.r.aii.1~ ~0.01~0-~o:oµo) =Nx.1000 1tJ~ t NS4*,"Qafl 6.o '! no t to.o *0.02.00 ~Q.;0 l 1~/Z.~ . Laboratorv -con/ro/ sta11dard:. Reference.standard*Tfu~~1ue* *1: Sitmp11?. . Hard~e~s(~ :1* WRS=.MVI'r\i xlOO number (iiV)* . : voilime ;B~gjn End *1 Total M:i.iltipJi~ 1* {mgCaC03'L) . . . .*<<:l~cptabl~ n1n~--i (.mg ....caco.9.1;;~ ...... : ,,{mil.. .ml . 'ml* . ml *=.90~U0%}l~'Sa1;.,.oz. 401 sc;. lO.;.u *1~1. J t<!l . I 20.0 fo5.01*recisitJn:Hatdness %RP.D= S~mpJelD BegiJ) End ;I* Total* I Mliltjplier (!JigCaO>Jll,). {(S.;; D) /l(StJ>)alh 100 rill .1111 : mt zoo** I ~ Dupllcate {B) l."l t '*'l"tl )fl-:1.k1 I ,1 -i~a a':'o J.s 110. e>. *Leto*.l Matrix soi/re recoverv: Reference ~nifard .1 .S~ilc¢ l~*'.. *1 Sa.mp.!~ : ~pi~ hard11ess{A)~e~in 1: Endj Total [ MultipI,iet:: .va* number ; * ~VJ .' , vo~ume* * '(iitg, (:!l'(::()jfL). * . (in~CaCOJll)).,, _(~J): ml:ml ml hl~~\'hnJ 4o ,: 1* 5b * *l~J ha.t *I Ll a I tcJ.o.* l?>D.$ampJ,ehardne5s (B) M~sured SJlike."'1lli.te(MVl %iR=MV1SVx:JOO(~gCaQQ#L) MV=A-B . . (accep:tahte range . {mg QC()j/L). =75rol:Q~) QO '-lo . 10001~ . I Sample 111easureme111$: Sa.mole number'.Blank .* $amJ>fe:.iD Sarrip::Jflum~ f B~f r~t r!~ r Multiolie~. . Hardness ... (~ CaCOJffil J TV=:ND{slioUld.be=O'mgCa©il:). 'Sb o.o I o'!o I .~:.o .20.c;>. .l\C I .ssw I oe.oq,Jk Je.1 1*io,t I t.oLfo.J lo6ot.lv fl I mHG:.W* ,;;to~t I Jif.~*1 t.1,;.e. 9t11:oo~oi. u~ g ~1-\. *a1;q 1'1.i-f I 4~*<; . tlo .~ J if3~o"f.\v Ul--z~Ji I I 1*1 sst;J -jl;c.t l Jl01 .Lf.* 4 *.4* -.~. ;.;7..J*..6 I. ~et. ~ ...z:.o*., B*t5** 40 O~-a.~.*~* f -m Hb.trJ* 1~e ltti,.flll~~ Cl2 J @* 'Z(o.4 V I f --cf )'.IJJ.w l~<:)*I &l~** *qo ,q~~~*I 4<llsl&::J.0 . ., 92.Q'}.t9_.U4 O~-~C'J.Lt1.O*D !*q~~\ IAet'IM Mn~~ Jltfl8 ~-"1 Nore: If >tSml ofti.lrant is oscd,,Sl\n)pie-~ be-diittteq.Page 58 OT 90 Reviewed by~ I '&. ..I Date revieWed I. .64-o~~ . I .,. / so P C7 - ~xbi~it C:7~~ *.~vi'si0fr01-01-n l *e g.~Page __z..._of_~--****b *~ C.'":~)l J. ~nrptl)tal~~'Wlo""b!C;.Hardness (SM Z346*C>l997}l ~r£**.** 1 1lL = 1.JJrng caCOIL Time initiated , ........._ ,<8$f " J)ate.anal~* *. *06.D5. ~y, _ Time cOmpleted . .........; l t1'1ra11t iiotmolJJv tuiQ*~1f11ltiP1kr*detemu'iliiiion:.* * :., .*~. : : * . ""' * * .,. ._.......k .. *J:iegin *~nti Tofii.J. *Notmalitj.(.tV) ti( EDTA pH 'Factor or Multiplierrilf~ei(,e * stanefatd* m1 *. ml . ...o.2'!E *'. . (Nx 50000}/ 50 ml sample* . tu.imber* * . *number (:E): .. * *'-le ~ngc ~ o.oiso~ o;onol -.Nx moo l Labomto!Y:contr.oistanlliirii: * *Rere-re~cestan~~rd True. value ~mple Hardness (MV} % RS= MV /TV x 1-00 num.~r (TV) . volume Beghi End'Toint Multij>l~et (jngCaCOj/L} (aecepta bte .r:ingc{mg~11COJ!L}* {mi}' mlml ml = 90 to 1 Hlo/o)*1 so.$a.mpk: ...Hardness %RPD=vofume Bi:gfu End Total Multiplier (mg*CaCOJIL) {(S - D) /((S+D)/21} x 100*'(* 'rid) mt .. tnl ml:l~ ' 61) *~;..( . 6cl 2.z i*io.o s44 .. , Diiplicate (B) 2;z.. -1 0 ---..oeost.ip ..J. *1 4 .; S.t1 ll>i0 'f4 Referencti starida:i':d $pike vnlue * .$ample Spike hardness (A) 'number . . * (SV) . "volume Begin : £nil Total (mg CaCOJiL) .. (me'caco.Jt) :(ml} mtnil inf JSampt~.fuirdness (B) . 'Measured spike value. (MV) :: * %R=MV/SVx 1.00 J {fu~aco.itJ:,JMV=A::. . B* (~cccptilblc range (ing CaCOJ!Lf = 75 to 125%)_J 40 IQO.lfJ.* .* Samplev~!Um¢ Begin *Eud . Total Hardness Samole-numbei : * .Samnte m . *(mij. Jril mt' ml Multiplier (m2 CaCOJfLl .l T\f===*Nn* *B .. l..~lj\; * {sn0!.'11<1,be=u mift;aUJ:ii.WJ ?,Un llti - L2.o Ise J Ll:z. - &JO J J J . SOPC7-ExhibitC7.I, revision 01*01*13 l 0~Page ~O-r,*v,,~ - II '~- '"'""*** I Page: .a: 9f *~1 \ L___i -:---* EmiTDP111tnl>l~$o!11Uons.lne I:Iard~ess (SlVI~34fJ .C-1~7}l Analyst ICJ6.,Qs.iy IN. RL .;;,. )..0 mg; QaC~/L . l Date analyzed . Titrant 11or111alitV and nr11/ti11/ier,lfetermi1iaiion:Titrant re!.A_. . __ ...Normality check Begin *End Tot~I ' Notma,.fy(Nj; iifEQTA .* .(;a Fi,ict9r or.l\'iultipltet*l~ ""0~211\t .*** * ,:= .(N:x SOPOO)I 3tht~ s;unple number number fE). (~~cptiblc ~~ = 0~0.180-lU~tl)* ...... _:_.. =NxlOOO Laboratory:co11trotstoi1dartl: ,Reference st.'lildtJrd True value .*Sample. ilardnc$s:(MV) .. %:RS:~tvITv.x 1011 number (TV) *, voJume Begin :&nd Total* 1"ftiltipJ1et* (0tg Ca(:i9J!~l * * {ttccci>*alile tnnie (mg QJiCQ;i/L}'. (ml)' ml* ~ ~*:. ~~~ so SnmpJe Har.dn'55 . %RP.n.:. Sample vofoine .. B¢gin End T$1. .Mu,ltipljer {mg:4,CO,iL} : {($~'D)./!($+D)(l)} x IOD number (ml) * . nil ml . *inlJ) *~.*. ;.;.J , , Li"'. .., **'4$.1:. , , I* l .. *. ~-s .*z.z Matri."I: spike.recover_v: Reference .standard .Spike value ' Sample ** ,.. $pak.& hjtun~ cA.l number * (SV) * **volume . .B~ilt. End101al (~g: <:acoJII.) *(ml! :CllC03'L) (ml) ml .ml', ml*..50**I Sl\mple'lmrdness{IJ) {mg*CaCQ.VL) Measured.spike vafu~(M¥). MV=A-'B .{iJ!aOit:OJILl % R"=;MV tSV':x 1'111 ..{1!¢~~le:rasigi; .. =..i7Sto*125%) .... ~J Sample meta11remm1ls: ...$itmpfo*.y,oJuine * 'Bigin. : .E~d : ;* f,Otn) ' .ff~)'.d~ess .Samote:tn tmn int I Sample number ml ml . . Mwtinliet', !miOIC:Ll_J 1 0 *.~ ,' , *. 1 I' z,~J J ... .... J \'J , ' :~ . :: [ *~a.~ r~viewed J* * . f>~.a~\f J *SOP C7*;,,, ~~ll?it<Cm J, revision P1*0.l"'Ul 0~**Page I .of b YI l IE:: ~. :;;t; j£il~~~~"'"' Page .1 Hardness (SM l340 C-19*9~Anfilysr J)ate:anaiyzeq,,-l;C>.--- *-* - . OOX;Jt,.* lV.r. ~.RL ,.,,. LO mg*CaC03/L. Tiine.foitiated Time completed Fl!~t\?)l-Sa..1-- -]l . . .. Tltmnt ;1pr/11'1lftJ(tl11<l n111itifiliet,f.teernlii1at/Qn: * . Titrant *1: No.m\iility:tftel:.k':j* .B,~gin1* End *I* Total NcirniaJity (NJ of'EDTA pH Factor or Multiplier l reference*.* standard.minibtr .. *. . ilfil.llber ** .mr *mt* ml tEl...i-0~2/E, (acccptilbJc rnnge"" o.01$~* ...O.IJll(I) . = {N); 50000)/ 50 ml sample ;::Nx 1000 IM~l tr¢5sl3a7:l o.t) __ ltt*<1* J*q.e a.01.otl ta4 I WtiriJJif!Y °(:ontPJI $/mtdai'.11: . . .:ifamriess (MV)Rere~~~~~~-ro *1 ** trtu. .~1-ye *.1*...:~:~!.%'RS= MV /TV.x 100 Beg~n1* End J* To~I Mattiplier I On~ OiCWL) (aci:eptable. range-i (~g G;iCOJIL); .(ml) mt .. *. mt . . nil '=90 to BO%) r"N.ss1x:i02, 4Q I so 1q~a Jn.~ t t'l 'tb~.J .aq q'l.-5/.., . ."iJuii1iCiltii$li.iitJJ.{ept~isiim: '&lmpl~ .Hardness %RPD= *sample;* s~~pJem . volum.¢ ...*- .cm'o *Begin *1* Encl ,. Total MD'ltiplier I (mgCaC03'L) {(S- D}/[(s+D)/21} x 100 nuiil~ ml. mt . ml *. 1'4*1S&c.H~~\ tr~ '\.. ' '51J I\~ I *~~. . I *~d *1;aq *1s* 3r '~lieme(~) j J~~ .*.1.~s~z' t'l- :.!. . ;Io . 3~ . ,; - 5~Co7.MmnxM>ike~covm: R~ferenC£i,*stattd~* *f * :Sp!kevalue .. , .SampleSpike hardness (A) oumber '.. ' ' {SY)*._. ' . *. v_.*.olu*-~e,. B~n1* ~nd **1: Total. I Mi!ltlplier: . (mg CliCO.llL}.*(mJrCaC-0.JL>.*. ___(ml)- . .011 :ml . ,ml.Jilf.~\~~J .. .qo ,L <<a.J Lls~s: 1 ..~.if "3.1+1 . . 'tQ.q ". ~*<a j 0Sample.har4nts!i-(B). (~cii¢6j11l) .Measure<l spike value (MV> .*. l\'IV=A*B 1 %~=MV/SV.x{ac:c:cptiibh: r:tugc 100 (!rig CitC:~L) =75.to125%).a;; 4il '02.s*1.I 8an111le. i1!.eaS1tte111e.ms:Sample* volume *Begin 1* End I Total HardnessSamtife number *l .Samt:ilelD . 'lnll}. mi . ' mt . mt I ..Muttiolier J TV=ND Bfank. (shiili!d'bli-=(hng Ca03'L) *t:."h..OU o.o JO.O: f:{)-0 I to.c-{ (mg C:iCOJIL)µo tbaf~7.;~* ***i~w~* L 1:~1 .1* ~.a:.:.a. ... *7*~ ,, u.a. . .. ,. -'I ;tJ. .. ~3 J Jfi. o~o~"'J".l J 1 3 l~ Jzo.~I J. ~*-*r ~*S"' . :u., ~*tri'. ~o;;;\ 1,f\f Catu. t. . . :~o~s 1.aa!1. ,I 3. y laG)I .J 1~aio\O\-. ~t-~ . z.: *~!j Il".f.bA *2.1 . t.oo **~OEOb.:ctL, l 3. ... ~~.oJ:-$0:01 3o I.II r\l*~"l.;.u), I :.s :6:<<-u J - 11,; *\\c>>utntt~o:1 Ir. t -~l j..~~o J"32,5. I .z.~l52~s-l'?>'S...;i. . :.io 61<DI l\$b.&.O\i..n . ii; J !J' **~ l'.38.;tl. t ..~. 63 J t~.oao:t~5. . :L~.wa:d1il . 1~ 10 . 38.1h41J-l3.o~I'J 310 Note! lr.>15mloftitr.mtiSUsc:d;sllinple'mum~edi1uted.. Review~ byt f 'g\._ * ** I -Date reviewed J O~*cM*\'=" I J Page 61 of 90 *0 SOP C7 - Exhibit C7.l, revision (} 1-01-13J,>ag~ 42..~e 2. of .3 l &rdn~$ ($M 2340 C-1~97) *RL = Lei mg*caco.;L l Tlttll11J 11ormall(V 1111d nmltli1lier determiutrlion:Titrant .Normality cbeck *' B~in End * . Total No:rmaifu' (Nfof El>'.fA *pH* ~a~ SOOPO}/ SO :mlsample number number-* {ae~ptllble*tatige-dl.Ot8!J:~ O.OllO) * .Ns. lODG**~ ,..'4:....., . . . - -~JCj ')i Laboratory co11tro/ stai1dard: Reference standard Truev;tfµe Sampl~ f!al-l'ln~~(MVV % ~\::.l\llV1 TV x 100 number (TV) volume Begin End. Total * ~u~fiP. J.ier . . (mg,OtcoJ/4 * * *(acceptableran~ {mg (;QCOJ/l,;J 'ml) *ml ml ml ,:;:?o.tUtO%). 40S:im~~ , . .Hardness * .* %Rrn=:voiume Be*.~..n End ; :l\'.l,ltiplieJ: .. {m.g Cad>Jj.L) ({$,, J))/t(SfJ))flJ}d.0.0 {ml) ,fitf ml' "2. .H>' '!3..J :q.O,\..J O*O '2;.°I Matrix spike recoyeTJ':Reference standard Sp!keval~e Sa~ple 'S,Pif(e: Ifardne5s,tA> - number (SY) voiume *Begin End Ti)taf lVIUI~plier * (ti!g'C:ac~>. (mg,CnCO..i/L) (mt} ... ml .. ml mi*.o.Q q.q' 5)20*~** *.500' ' ~SSt'iOZ. .x:.o~ Jb, sample 11arp)ies5;.{Jl}(mg CaCOJI~) -'{.~Measured spi~value(M*V'). MV='A-B.o/.(R~'l\!IVlSV'~*lOO (acc.c:pfabfe ra~~*lme.C:\CoJLl ='7$,to US%) J Sample melisaren1e11ts: sa~J>.teyoJumeB~gm , $,id 'total ,.. *aardn!!s&fmJ): . mt . MultiDner l Sample. number S.imiJJe.JD '.nil '. '.111i (ma::Ca:CO.JIL) j J "10' t'l. *-.' ' J *ex,.' J I*soP C1 ~ Exhibit . . .. Ci. A. reviSion ol*Ol-l3l Page '-13 Page S of 2 l Hardness (SM:a340 C,1997) l RL = LO mg CaOOi/L . l.~=1 til)el\I ., _ Time initiated "time completed I~t8c)oJ'f. ./I.itt:imi uol'm!ffiiiltzndi11ultitiJ let t1,eieni1i1101ion; ..,~ ..i *. normant_Y.check 8¢gtn * ~ua T'otiu N9nna*it.r (JV) of EDTA pH Factor or Multiplier reterenc~ * *sfu~d?ru, * *** * ' m( nil . '""*O:if.E ' - (N x50000)/ 50 ml sample number\_. . -'nutnber .ii!>* flin~co:>O;o18o""O.~D) .. -Nx 1000_ uE'.lw;11.J Ut/Jormifrt~,-cJ>lilri!lsta11a11rd: Refer~ce-:sfandat1f *Truin*al'Sample* Hardness (MV) * %RS=MV /TV x 100.. nmnbet* Q:vj* , . . voifii'ne ,B~gin Encl total l\fultiplier (mg C11CQJIL) (11ccept:rblc range.l ' .. i* *.. '~_ll!g_, -CacbJIL.1 * */ ._{-*m*ll ._ ....'** - ml ..ml =90 to110%) 1 ' .:S:tmpte *_. Hardness %RPD=Sample ' vl'.!l~me Begin End Total Mtdtipl~er * (mg CaCO.slL) {(S - D) /({S+D)/2 f} ~ 100*tnuii:ber ("ml ml .* . nil ... ml M.atrA;siilke tlicovery: .Ref'ert!Jlee s.ta~(Jartl ' Spike.vnltie '$tritple ' Spike hardness (A)number : >~V)* * * 'VDltnite , Begin 'Erut 'I;otlil Multiplier . .(l!lg CaC03'L) f~*'CnCO.JfLl. {ml) * :. ml. ml . nil j 'Satnple.Mrdne$s cB>*(ufg:Ci!CO!!lb} ,M~asured si>ike value-(MV)MV=A-B%R=MV/SVx 100 (acceptable rnogi?(mgCaCOJ(L} =15 to 1:?5%J- ero e~ 10561*J Sample vc"lu'ifie Begin EndT~tal .Hardness ml rnr! (ml) ml Multinlier (mg CnCOJIL)J z~o. j .aos J --~ - :716 I . _.. ....... J ------ .. Note: Ji sismlofliliantisUS!?d~sampl!:fmUStbtdliluted. Reviewed'bv: Page 63 bf 90 * * *** ** .1 I .........,--11-r~~----'-"~ *I Date revi~d .... I __o.:.t....;;*{Jr_\"1_ _..J 'SOP C7 - E.'chibit 07. l,. revision 0 I-0 l -13' Sequoyah Nuclear Plant Biomonitoring August 02-09, 2016 AppendixD Reference Toxicant Test and Control Chart Page 64 of90Pimephales promelas Chronic Refe,rent:e Toxicant Control Chart Organism Source: In-house E!'lvitonment"t 'testing Solutions, Inc;LO ~~~....,.._-....------.----.----.--..,.--..-----r---.--.,........--.-_,...-.....--..-----..-""T"""-,....---.USEPAtontrol limits(+/- 2 Standard Deviations}'0.9 ...... ' , ~-- *0.8 CL7 0.6 0.5 1.4 'U$E:PAW:arning:*a11d Control tirnits(75th and 90thPercenfile CVs) u ~1~2 1.0 ** . *** . . ... *********************4*******'**,,.** ******a** ~~t.:.::~.:..:. :::~-~-*~:::....~ ;_;--*-***~**-**-;.--"~-**- ~--**- . *tlO ~en N 0.8 O;~ m "ti ....;..;._.,,,.._.;...,.. ~**-**-:o~~-*-**~*~.1---:- . . ~*~*-~**. .,-.. *--~*-~--~*~'-*."f,- ( OA .,................ . ~ *****D************************************~******* . ******** Laboratory Warnl~g and Control Limits {10th and 25th Percentile CVs) 1.2 LO~ .... *-** ****:Ii.*** *** ***.-* ...... 11-:*.... -.:**. * ....................... *~ ~* ~ *** ., ...... .. ~ ...-...._..,.. ............ ~********~*********.* ~* ......................................... 1!'*0** .. *** ' -0~4 Test date *7-day rc 25 =25% inhlbition concetttratil)n. Ali estimation of the: concehtration of potassium chloride that would t:a~sea 2S%redoctiofrln Pfmephafesgrowt:hfortne test pqpulation.- - tentralTenderit::Y (mean IC15) . warning LitnitS (mean 1c25 +/-.sA.ld or sA.75) Contrc(! limits-( mean rc25 :!: ;sJ\.25, SA.90, or 2 Standard Deviations) l Q) Pimephales promelas Chronic Reference Toxicant Control Chart Q) 0 "'ti <c-P* Envlroilm11ntal Testing Solutions, Inc.<::> Stoteail.U.O~EPA laboratory laboratory use PA USEPA.Test number Test date 7*day1Cu CT s control Limns s..,. Warning Umlts s..,. c:onttol limits s.,,* Warning Umlts s.,,. control U111l1S C.V 11!/L ~Cll (a/lKCl) 0**25 CT~lS CT 0 Si.:10 c:T+S.1,;o* cr-s..,. . cr+*s..,. .Cf*S~.u .CT+So.11 cr.* sA:.. a*s*.,.l OH!Ho 0.74'2, ;j)3*0N6 0.67 0;10 o.os D.60 0.Sl 0.08 ~.62 0.79 o.1s 9,51; o.ss a.21 D.411 0.97 0.31 0.$9 l.Q2 0,07 3 Q3'0H~ 0.58 D.(!6* o;oa* o,so 0.82 o:os o.ss 0.14 0.14 ci:s2 o.ao MS 0.41 o.9.1 .0.30 o.ati 0.96 0;12 4 03*04*16* 0.81 0;10* £1.JO :o,so 0.90 o:os* o:62 .citlS 0.15 MS o:a~.- o.~1 o.~ o.9i; o;n 0.38 1.0t: 0,14 s *Ol*OS*i6 *0.61 Cl.68 o.o.!i 0)19 0.87 o,oa *o.eo o:~a D.14 9.S4 0.1!2 .0.26 *o;4i 0.94 o;~i O.llT *o.99 0.14 '6 04.()4-iii om M9 0:09 a.s2 a.Iii; o:oe o:si rm 0,14 o.s4 o.~3 0,26 D.43 o.9s o.u 0:38 l.00 o.ii 7 ~UH!I o.7ll 0,10 o,oe Jl',.53 l).87 b.os O.GZ 0.79 0.1S 0,55 o.ss 9*2? ilAf 0,97 o.31 0,3.9 1.02 i.J.12 B\ os,irfo; *0.7S *0,71 o.os o.ss 0,Bf 0.08 a,~2. o>r& o.1s 056 0.86 b.'il* 0:44 o.!ia* \l.32 0.39 1:oa 0.11 9 OS*lMli .0.94 . o.10 0.08 0,54 o:aG 0.08 0;62. 0;79 o.1s o.ss (j,85 .9,2~ o,4s* o.97 0.32 0.3~ l.02 O;li io o~'Ni. 1).?q . ci.70 0.01 o.ss O.liS o.o.s* .0.62 1).18* .o.lS .o.ss il,a!i o.2'i 0:43 Q.97 o.32 o,!9 i.O~ Q.li u .tlli'l4'16 ll.14 *lJ;7o 0.0? o.ss o.es o.os 0*.62 Q.?!l O,iS o;!fo a.as 0;27 0,4,4 .0.97 0,32, *o,ag* .l.02 0.10 fr !)7~12;16* *0;fl1)* o.n 0'.07 o.si O.ll6 9.09 0o1;3* a.so o.~5. o.56 o:se 0,21 0.44 .i).98 o.ai 0;39' J.03 0,19 j3. Q1*19*l~ 0:63 *lfo o.ci:i o:s6 *0.85 o.oa o.6t D~79 o.is. .o.so. 0.85 1u1. o.411 \).97 *o.32 o:a9* 1;02 0.10 ~~. .'Ol!*OZ*16 0.79 0.11 b.ti7 o.s6.* 0,86 o.o~ o.~3 *\1,80 O.lS Q,~6 o.ee .Q.27 0.4'l Ms 0;~2' o.~g ~*O:l' ll.10is l(\17 18?9 to 'Noie: .1-d iCu " 'r*day lS"' fnhib1t10n cc.n,enrratfcn. /\n e.stlmallo~ cit. th! concentratlcn of pota5shin1chlartd&. th;t ~ufd camft a 25% r~d~tW11l Jn Pll!'l!P!litiel ilicwtb fo.r the teJt pojiular;o,.,,q;, Ci!nt~ftiinde.11cy {mean IC)j). . 5;, ~!~ndilrdil,c~;a110~, ai t.lie 1~11 )latues. t..a~oBtoiv C:on.1tol llnd Warlitng ~iiillts* .

., * * . Labor~rory:cimfroi.~nd warning !inl.i.ts wer!! il}~a.~il$\!ed.11~hiQ lhll $(a~dardda~ietion 111 the ic,,,v<).lves corr!l'll~nd1~g1oth~,1Pt~ amd5tii p~rctinillo Q'$,, The5.G tan&

int. * * * s.,tii:"' Standard iJ&mil~n ec1ra$ponr11ns io tile-Io!~*p~rtentifo cit. isA.ra"'o.~21. S~u'= Standiitd .de~/atliin'i:orr!itpbndlnir tii tlJe 2$"' per~~11t1l1,t tv, (s,Jj ".o.2ij iJS.~P/i. i:o1m:olamLwaroln&tim11s * 5.,,. ~* sian~arcl c!.iv)~r!op cilrrespoiidiri[Ha lh11-fli~1' iier{~lil!./;\I. {S*,n" !l~lll SA,.,;~ st~1ilari1,dij~tailii1;,correspoiitlln~,t~tli~ !lo~ pet<;iilitil~ cv. (s.~ D.4Si 'Cy"? .Coefiiit~ntof var'llition or lhe ifi> valu~~. tisEP'A.,20~0. li~i!~r~~ridirijt~il)\<<O~'!i~for M01~odya~ib11;1y10 ~!~ i!ffbiolilTOiit<IYAppli~l4n1 u~dor,ih* ffiillll)ll.~ollutal)i'Dil<:h1rgi ~liioio1iilll) Protr*m,.fi.1o-u31*!1*ll~Mo3; us Enoiranm*nl*f ~omifo* ilf;eney;~n~ll. Olt *;.F Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source:. In-house -at* 1.00 r; *~**** ~** *.*.***,..................... **.<'* ***********.**. n1 .... t! *.~ .;!! 0.75 ii I!) c;*~* *- .. ............ .c ........ *= o~so ~-~~ ** *~. ~ .... "'""""'~~~~I!!'*** **** **'****:* .. 8 E~ . " ****'**** - 0.2.s. ~--";--:--,.::;-.*~*~ .. *--*~- . ~---*-*,.,-~~-* . -*.. -*.,,-..;_, .. _.____ __ ,,._. ..... ,~_ ... *~**-. *-.-.,.--*-**-**-**--*** USEPAAcceptance Crit~ria (> 0.25 mg per surviving,fai'vae} -z ... ~ 0 r: .;C: ~ ~0' Kentucky Acceptance Lh:nit (<30.0%) *;: 0 !ti m I!) 20 0 0t... ....s:: c 0 cu u *o I;.. fEcu .E 8 . .... ~ ..... . "*"."' ** ~*******--- ........ ~ ............. * * - * * * * * * * * * * * ............ ol!** *.* ~o ~* Cl 20 U) ~* Q,. 10 t:onttol lleprodu~ion, Coeffident of Vari;ruon (CV), or Percent Minimum Significant Difference (PMSDJ* ?Msb Is the *rninim um signifkaiit differ~nce betw~en the control anci treatment that can be deda17ed; statistically stgriificant Central T~ndency {mean ControfGrowth, OJ, or PMSD) -COntrol:timits' tm.eari Control' Growth, CV, or PMSD +/- 2 Standard Deviations} e Precision of,Endpojnt Measur4aments ) .... **-**** '*'* Plmepha/es prome/a$ Ct)roni~ Jleferenc:e Tt.Jxitan1 D;;lta Control Control Mean Test number Test date Cv Sun/iva! ' 1 G~wth. Cf* 0: **MSI> l'M~n ct for Control-Growth' for*Qlnirol {%) : {rrig/larv~el <91-J, (%) forPMSD(%} lmJ:IJarva:ei ,Gi:oWlhC\11%1; 1 03-01~16 100 0:56~. 7E 0.07 11"8 2 03*02-16 100 0;1~~* o.66i l.'3. 4;6 0!07 8.9* -10;4. 3 03*03-16 100 *Q.f.f71 0.664 5.a s:Q o; ,f3~4 llA 4 03-04-:16 100 0.5,1 .0;646 3,1 4.S !),()$ ~35. li.., s 03-05-:1;6 97.5 0Ji30 9.~43: M :5.z o:os. 8,1 11.2. 6 04-04-16 100 o.451 <Mi~ 5;!1 5.3 O.Q4. M 10.7' 7 04-05"16 100 0.647 -0.611* 12.3 '6;3 0.11 17._6 11.7 8 05-10-16 100 0.541 O.!'iOS 1:0 6.4 O,QG *:u~a 11.7' 9 05-17*16 1()0. 0.,5()7 0$7 L$ 5.9 M4 :8*~ 11.3 10 06.-07-16 100 0.421 o.s19 5..0 5.8 Q.06 ~;tU '11.6 11 06-14-15 100 0;<155 Q;5$ 3~2 .5'6: Ci'.()3 :7,3 . ll.2 12 07-12-16 100' 0.5.78 0~569 s.s 5.9 .0.08 ~;i* ll.4 13 Q],.,19-16 ' 100* '0.774 '0,!?84 .lQ.l 6,:a o;U :i-a.-s. 11,5 14 Q8-02-l6 100 0.570 o,ssl 3A* 15-,0 OJJS **a.~ 11.3 15 16 17 18 19 20 Note: CV= Coefficient of,variation fot conrrol'growth*. lower CV ,boond.!jetermined byJJSEPA.{10"' percentile)"' :35%. Upper CV bound ~etemii11ed by, USEPA (so"'~entiie) =m% MSO =Minimum :Signif~nt Difference PMSO =P$'cent Miormum.Slgnificanti;>iffefl!nce PMSO Is a meawre.ofiesfJirei:ISion. ThePMSO is the minimum p~tdifferencebetweenttiecootrol a<:idJ:reatmenhhat.caitb~. det:t~red statisth:aUy slgnificaotio awiu>ie-effiuenttollicii:y test. . * . * * . ,, . .. Lowei"PMsOboundde:te~m\ned by 0sEPAJ1(1"' percentile~ "'12%. UpperPMSO hound,d$termioed by:USEPA {9(l1h petcefitile) =30,;,; CT= CentralTendam:y !meai!.ControJGrowih; CV,l>rPMsti.) USEPA. 2000. Undetstandiog :ancl Accounting for Mi!t!locl Var\al:llhty In W~ole Eff.lue!'lt ioxlcitY .P,ppl!cat\Qns.1.Jnoer*tl'>e~National-Polturant Disi:l~ame.Eliininat!qn Pn:>gram' EPA-8~3-R-00-003. US Enlilronll)ental Ptotectio.o.!\gel)cy, tincinna,ti, 01:1. .' ... ., . USEPA- 2001a, 2001b. Final Report; lntei:!aboratory' Vartabllity Study of E:PA ~hort-ter.mCIJronic*and f\c;ut.eWho~~fflt,tent Joxl¢1tv Tajty\ethOds-; \lo1i11rtes f._;ao9l . Appendix. EPA-821-B-01-004 and EPA-8.21-B-Ol.005. !JS Enviroorrieotal Ptotectio11 A(len% i:Jncin 0a~i; OH. "~--* ~,. Page 6Bof90 .O*. a* Page 1 of6 -~,, Pobssium CJdotide: Chronic ReferenceToxi<:~ht Test .tEP'A~82l-'R4l2.0i3. Method IOOJU)) ,i . Species:* .Piniephales primtlikis ' . PpKCld~ Tem:Number: 14 Stnck r~n:. so g K~llL; .. . . . . .. p ** * DiSSolveso ... K.Olml4..Milli-0\vater.. i:t 15* 18. li '.98~ '983* 9S2 m.* .igQ(t 1000* 1000 1099. In.;.house .cilltill'e * Iifoi1bator number and .* sbeJftoeation: , ~~(>\4l~ '-~?<? ~ *D,rymg l1tfomu1tionfor weight . .. .. Ot**~~..,~, : b\O\ : d1itf!n1iftiatfon: .*:pB>= ~.~b .. Date/.Time in c>ven: .T:~'tttre,: LS-.\ :. Initial *oven temperature: io~11 lliL.. or Date / 1iime oU:t oven::. 'final QV,enJerri *eraiure: Total:diylngJime: 'i.. 1Jii,ilyfeeilil!g ondrenft1Jiaf iiJfiJ111iation: Test. ir1itiatiori, tenewal, MHS\Y or termination batch used 'J *4 ', 7,* ' *<::hV Page 69 of90 SOP ATil=ExhibitAT2U,revision 11-01-14 l ~i"' **:~;*r*;i )~*~~*~~~~~Sol~::.. Species: Pimeplzales promelas 1 Survival and Growtli Dma Day I IQ* 10 JO 10 JO to to io I io *1 J in K . 10 . L. lO* l l .Jb ( 0 ' (Q l t> , I .t>. . ft) ; .( {) 2 lO* t 0 . IQ . IQ (C (Q I 3 /'Q t*o* tp . LC.* tq * .ltj lO* liJ. JQ, IO 4 *lb to to lQ* lO 10 .*19~ l(:) IO s 'l0; ~o d.() Jo. ,Q, l(:). fO. (0 ltl* I(.): lb :lt) .t\l JO lO ro l () A = Pan weiglit (mg)a . Tray color code:: _I q VCo dil Analyst: J1'- . ;t3.ol s.63 t&.S\ *J1,,.ti. 1~;4!~. J3.11 l4~tfq_ ls.le I~~- f:~ J~.t~ tts.43 Date: "-l:;?x').\!11 . * * > " C =Larvae weight (mg) = B -A Hand calcuL'!ted. _1 Analyst: _ __,,,V\~--~- 11 Comment codes: c-= cieL'J"~d =dead, fg. =*m~~ 'le = .idll~ ~ =ilii$.sing;, .~ =;;,gl~k,,sm ,.;,, un~ally smalt lg = unusually large, d&r == decanted.artq :returned, w=:wounded. Comments: Page 70of90 0 Page 3 of6 *~ J \ &Zt~~~/ .Species:* P1m~pltatespromelns. Ppf<CJCR Test Number: 14 SimvivtJl altd Growth DUia 900 lllP KCl/L *.Q :R: ..*... s* T u v w x *10 JO to: to l() JO m io 10 10 10 J:' . lO: *. ro (,'Q: JO 'rt) ; ' tb *- ll:)* 10 lO ; tO : -t {) I ti: f.D ID f() to' *iO IP "19 lO 'l;O*':{;O' gaJ .* ~* . ..... ***-**** .. - *~*~ s~ 1 .,.~ *-u. ls> JO 10:: -6 e -:r- r-....... (p ~ b ~ . .J;. .*. if.); l () b ; l'i~~- ~0.1:1 ~~St ~&.ftt.l 11.~- 2*1~6'~:. *J-~1~ ~&..1'f fqlf1 l'l,1 J1J!.f 1g.£lq c=r.ame-weigJir<miJ>"".B,....A Haild .~kutaitd;;: Ab~tysl! . . * .J . . Average w~igbtpe:t i~itlal .*Pcrcecnt iltiniber l)(lnr'v:lc (r,llg)' *ri:ifoction* trom contr0l * i ~"" o. ~ u .. S\."\ l {%). . .Con111iditS:. Page 71 of90 SbP* Ari] -Exhibit AT2 J~I, revision J 1-01-14 Pag¢4of6 Species: Pinzepflalu promelas . JOSO:ni Keitt Y Z ' ' AA * .BB , tl 10 lO lO 10 *iA.. .,~ 2 3 b 'ti\. Sig ~ -~'~ ~"~"G\ ~~" g1A. g -~ g* ,4 3~J 4~~ *fl;* 0 0 '.\ lo s~ 11* 31. .*,al *tti' r;. *'-l~ \ "' '1.. ~ .~ \ ls.6'-/ *I s:Jt> Jfi.tf\. IS.b"': n'= Pan + Larvaeweight (mg)* An;ilyst: Alb . Date: fl&-11-li c =Larvae weight (mg) ""B..,. A lfaod* calculated.- .. Analyst: .~ Weigflt.pcr inl~inl number. oflarvitc {mg) =CI Initial number oflarvae Hand caku)aled. xl Analyst: __ _ __,_,lP----- Average weight per ~nttfal Percent number of lll.m1e (mg) .rcduc'tion from contr:(!l .(%) Comment codes:- c = cl~ar, d = deaQ. fg:=; J.ilngll$;k*= lolled, .m = mj$~jµg, lg*= unusuaUy large, d&r=decal'_ltedand renrffied, w=*wounded. * *= $irik. sm = unusually !:lmal,*.. Comments: Page 72 of90 Pimepha/es prom.e/Qs C:hroolc Refen1nce T9xlcanfT~st EPA-8Z~~~*o2-0l3i l\l'li!thod 1000,i);: .Quality COnfl'<il. V!!rlfl.Cl!tlon of Data Entry, Calt:ul~ttons; iincfStatlstlcai ~na1Y$~ tau~*!Dhef!_. _______..,....____.'4_ EtMronroenfal'restlng Sollltlon$, Im:; *T1!$t ifQ11s:._______._11_u_gils_**_1_0J.._o_*~...2_0_1_&.* *;1&11ahiu.n1btif~I ** JJ(illnvmbn~; A.*~.tft-~J4i'IUihl) :a,.h'f'l.*.laf'ffa ~rwu't!ilif\l(~*)~ VJ'tl~1/J!'ivM~.ttC.* Mt~nwat1ht/~ crifffldanfdfvirl.at)Mt W.ti~i/11'1':t&l~li'ibor~~ M**U'f~'{" Mo!'.w**lf!i/JnltflJ ~ffltftn\of~t.l~t1on *hmn;Ha1h1tif4nhom *~~'~ 1it¥11 ~~!ib\(~111 hA ' rttt~~t6lt&ttH.(n11J ~kMb*ro.tt~rmj_J t'i!t""'~'p.f"'~ * .. fmtf~rl *!Wftlht1Jitnu1 ffl). . . 11~1'*to:tf1~ fmll mr ~lrol.tHt* . 11* 10 10 .13.0l' 18.87**. S;M 0,584 *o.584: .. *B. 10. 10* 1S*.S!'. Zo.9,S SA2 '0S42 u. 0.542' o.na Coiltri>l 0$1p 100.0 M Not a~plf;abie, c 10 10 .15,51 2lil3 .S,82 *. *o.se2 .. -l).58~> *o 10 .. :10 .. 16;12*.. 2i;l!4 l;.7~ ~ 'g.572, o;sn* E* .10 10.: ..,)5;96' . .21'41' SAS, .... .O.S45. 0.545. !loo F let .!O 13,3!1 18.85-; *SAG O.S4& .. O.S4G. G 10 *10 . 14.49' 19.77* .s,2s *o.S2S 0.!;1!6; i.8 *o.s.w. 1o0.il 0.546 2.l'i* 4.2 H. 10 10* lS:lB* 21.43,; s.65 *0.S&S O.S6S* ... 10 10* *.1S.4o* 20.n .4,71 0.471 0.471 ~so )_ 10 10. JS.17* *20.ss .. s..a- 0541 !i.53! 8.0 0.541 .. io~.o o.sn* u.o: '&.$ K .10 10 !6::U. 2l.S6 S.64 O.SG *10 *10* . 15.73 20.ee. S.1S O.SlS 1'.Sl.S p 10. 10 "1S.9J: 20.64 4,72 0.472. o.472* . Q *10. '9' '15.14 19.9$: 4.81. O.Sl4 0.481. 750 R 10. 10* -16.44* 2.t.54*" s.io o.s10, 0.518 8.l li.S10 92,5. *0,477 .S'.J '16.4 s 111 10 15.28 *!JI.Ill 4,54 0.464 o.464 T 10: 8- 16,23- ~G.74* *4-51. . 0.564 0.451*.. u 10 6' 1G:4S, l~.19 .n* 0.457 0;27-J ~OD V: io* G. 1s.os*. '17.97 Z.S!' o.4al 0.4.80. 10.7 IJ;l$;- 51.!i !UR 4.6 s~,g w 10 s !4.J9 17.!4, .2.75 0.550 0.275 x .llL G l6':lG* .. .18.94' 2.sa*- ,o,430'* o;:>.SB y 1a r 1s.s4:* 16.4S 0.61' *o.s10 0.061* IOSO 'l 10 15.EQ .16.78 ..:... us:.* ;0.590 *05?3 6.7. O;Ua .. ns 0;09~ 48,G B;t;D* 1111 10* l. 16.09 i1:ss* l.56 . 0;520. 0:156* 118 10 . *is:o1: .*1s:G4 O.S7 *0.570 D;OS7 Ounn-~lt's MSO vaiue: 0.0414. MSOo Mlnimum_s11niffcani Differ~~<* PMSDt 11.J; PMSD" P~rtent Minimum srantfi~**t Dltlereoce PMSb lj ~ motiu(t of la1t p,..tslon, ihe_ PMSO fl lhb minimum peKtnl dlfferen{e '1e!Vleen !he oontrol.*nd 11.. tmenl lhat.Clln b& d<<ll.W st*lfsllCllfirsltinl~nt In a wh<lle cfflUeril w><iCity L!tiL LcwerPM5b bouf\d.d*larmlri6d l:tl. USEP11.l10th fH!m!llllll!I "121l. Upper PM~!> bllllnd de!ermlnl!d bi> usSM (SOth petcentllo) s 3W. .low*r ~*d up""r PMSO bGunds Wl!fe determined from ihe 101h ond 90th p*rcentli~. respetilv*Jv, of PM5Dd1t1 from. EPA's WET lrtlttla bor1tory Vanabiltt1.5tudv (USE PA; WO la; USEPA, 2001bl. USEPA. 2001,, 200lb. Final Report: lntorl*borotorvVariabllflY StudyoHPAShort-term Chronk; and.Acu!o Whol1 EfnU.n!'fo>diltyTe*t Mothods, Voltimes 1and2~Appandllc. EPf\>ll21*Mll*Ol!.4 *nd EPA'321-/Hll.Co.5. us Environmental Proletllon Agency, CJnclnnall, OH. ~* !!~~~~ \,~~ ~,~,~{* ) ~:::~1;:-L-GSQ~~~~ LarvalJFish Growth,and survival Test-7 Day survival* s1ar1:oate: *~2016 TestJO~ ppl<CICR: . .. Sa111ple,!t>; ~EF'R.ef:r"oXk:an! . End Date: 819/2016. 4b IP: :ET$*Enlllr.. 1'estlng sot. Sarr;p!e lype; t<CL;-P.otasslum ehloride. sample Date: * firotoc:OI; FwCHl'M!PA"821*R"02-0'i°3 Test~pecle$~ PP*.Pirile?.hales:prert)efas; comments: Conc*mg/L D-Control 1.0000 1.0000* .).. 0000 1.Q()Oo 300 *Loocio 1*.0000 :;.oooo 1.0000 450 UlOOO 1.0000 *.1 .. 0000 jJJQOO 600 1.0000 1.0000 *:l.0000 1.0000. 750 o:sooo ~.oooo *1.0000 o-:ao?Q 900. 0;6-000 0.6000 ::o.sooo O.OOQO 1050 0.1000 0.2.000 '_0.3000 0:1000* N11111t1er Total Conc;.mgtL Resp fllumi:itr D.,Conlrol 1.0000 1.0000 *1A1;io 1.4'12(1* 1A12p* .q;ooo. 4 Q 40' 300' 1.0000, *1.~lOOO 1;4120. 1*:4fW 1'4120 O:OOO :\l 1~coo l(l'..00 ti 40 450 1.0000. 1.0000 1.412'> 1;4120 1.4120 o.ooo 4* 18.00 Hi.()Q' 0 40 600 750. 1,0000 0.9250 1;0000 1.A1~0 1;*;i:Zo 0.9250 1;295'1 1.1071 1A120 1A120 o;ooo. 11.347 4 4 1iu:io 14;00 10.()f.)* 10.po. A 4o 3 40* o.s7so o..asos -o,78s4 <i.8sr:;1 5J!47 4: 10;00 ~g2g "900 o.5750 17 40 "1050 0.1750 .0,1750 Cl.4217 0.321!;\' 0.5796 29-JiB:t 4 10.00 33 40' AUXIiiary Tests: .*Statistic .. Critical Skew.. Kurt * Shapiro-Wilk's Test indicat~ non-n.oi:rnal 'Clistribution (p*'<"' O.Oi) 0.83328 .0.896 Eguallty of variance cannot be confirmed HyPothesis Test*t1,tal1; 0.05} . NOEC LQEC .. ChV .TU .steers Many-One Rank Test* . .750 900 *s~1,584 Treatments vs D*Control Ma.)clmum *Likellhood'*f'roblt Parameter* Value SE 95% FtduC!ar Limits eontrol. .mi1,.sg Crltlcai P*valutf. . M'u Sigma ltet. Slope. 16c4547 2.45609 't1.640S~21.2.686.*** O" Q:OSSS 9.48773* 0.99948 2.~7.Er 0;060.77** 3' Intercept -43,?a.5 i2694s s11.'os3 *z9.:;a5 * * *.. ** * * TSCR 1 1.ll.--------'-'--------=----. Point .Problts mg/L 95%Fld1.tc1arL1m1~* EC01 EC05 2:614 665;892 580.'9.01 719.902' s:35s 732'522 ~62Jl69 777.202 o.9 D.B eC10 3.718 770.725 .'t10.4~B 8?0'416 (l:7 EC15 3.964 797,~2 (43J'~4i 1:134.25 EC20 4.158 61S.563 i71.149 ,854-~ ~ 0~6 EC25 4.326 839-059 794.7:j'i 87;2.396 ~* . o,:.O.lr EC40 4.747 889.993 *853.898 923.$73 EC50 s.ooo e22.1i1 aa8.6s1 ss9.741 ~i,o.4 ECSO 5.253 955.3138 /920.64' ~900,43 .0;3 EC75 5.674 1013.$8 97*2.105 1.077.81 O,:!: ecao 5.642 10.37.*36 ~2.01.3 "1.11:1.5': EC85 s.o::i& 1066.-03 w1s;2s n5Z.a6 o,1 EC90 6262 1')03:2~ 1044,63. *1201;71 . j o.~+..-r;;....,;,,-.,..,.,.,..,,.,,-,..,,.....,,.,,,_..,,,,.i EC95 a.645 11s<F7 1q~a.11 12s.s.o9 '1' 10 too 10~0; ;oooo EC99 1~s26 12713.91 1174;93 ;147s,4Q, ~o~m!:J!L Dose-Response Plot. *s.~~~o--~-o-~----:""",,,.-~..,...~~~~-,---,, 0.9 *0.8 .. Q.7' ea.a** ell 0.5 ~- Cl 0.4 "'*o.a 0.2: 0.1* o .....-~_,..,,-,.~~,.......,~--.-~~--.-~~.,,----1 0 E (i 0 0 0 0 0 o* Page 74 of90 ~,., StatistiCal Analyses .~~~ ~*~ ... 'Sfart Oat~ ;i!f212t1\5 'Te!ltJO!'* 'P~ClCR sample 10: REF-Rer Toxlcanl 'Eru:tl_)atii!. . si9120~e L;ab(O:; Et&-Eovir',~t!i19 Sot, saritpleTypi;: KCL.*Paiassium*ch101ti:1e StlmpleUale: P,Ptoeot 'FWCHR-EPA-821~R-02-013 Test Species; 'PP-f'imepbilles promelai; .COmmei>?s: . 3. .4 O'<lontror :-0;5540 0:5420 *o:se20 o,5720 . $00'* 0)5450 a:s46o 0.f!~ 0,5550 4so 0,47.10 (l,5419 tt5640 0.556il 600 0.4600 .o.4410 0.5150 0.4720 7so OAil10 o.s'ioo .0.4640 . 0;4510 900* 0,;>;740 ,0,2590 0:21so . o.2sao. 1oSO OJ~S-10 .o:Heo 0, 1580 !M)5/'b *rrariSfOfrll::Untr.:instol'l!'lad* '1-T:iilac:I. Isotonic cc:inc;stlt .llilDi:ln 'N.iMean *Mean Min* Ma" CV%.. 'N .. t-SW CiitiC31 .MSD Mean N-Mean *p.qonlf'Ol 0$(00 '1*.0000 0,5700 oo;S4ZD* 0,!ig.(O :3A02 *r 9,5100 1.0000 '300.* ct54eo 11;9579 {);&~SQ !Mi280 0.5550 '2:770 4 1.'194 2.'360 0,0474 ();5450 0;9579 4~6 o.s33o 0.9351 tt533o 0,4710 9.5i>40 7.-958 4 1.84i 2:350 0.0474 0.5330 0:9351 "fjQO 0.'4726 ~;e2li1 0.412:0 OA4iO* *o.s1so 5.6.ia 4 4.a77 2;360 0.0474 0.4743 0.8320 ..,50 U.4765 O.B36tf 0.4'1'65 0.4510* 0,5100 s.349 4 4;653* *2.3W 0.0474 OA74'3 0.8320 son ~.~7,4,0' '0,4e07 02740 1).2580 0,21!90 *t626 4 0,2740 0.4607 '!i:lso D.0980 tf:171,9 b.o&so *o.n57o 0.1/360 48..631 -a o.n9ao 0:1719 Avi<llia!!Toms* Stalistil:' Critii:al Skew Kurt *~PJr9-'l/V!lk's Te$t.1!ldJ~~ !'lorrn~Jdisttjbu1iofl *CP > 0.01} 0;97576 o.asa '"0;5272 0.54083* Baril£.lf.sTest lm:!ieates.e9uat *vanancii!s:!e .. o.s1I .3.Zls4s 13.2767 liYi>QtbesisTcistf1*tail,O.Oof** NOEC *,l.OEC. ChV:.* TU. .MSou*. MSDe MSB MSS. . F.Prol:I df Ourinett's Test* 450 *:eoo 519,!>15* 0.!)474'3 0.063:£ O.l>Q754 0.000!11 *s.4E-04* 4,15 1'mll~lln1s 0-Cooliol Unear lnterpolallon {200' Ros~plss) .mgiL. SD 95% CLISXPL .. Skew*... ICQ!; 351.92* 92.SS* 125.98 -579,-:;3 0.0791 1c1c 50'1~06 46.as' *1104.90 Stltl. f3 ..0~7:.99 IG15 .513;e3 1~6 459.54 . fiS.4.<!3 1:~226 *1.CJ*,,,.,- - - - - - - , , - - - - - - - - . IC20 763';67 38.:97 484,61 '7S1;91 -s~7Z44' 0;9' l~ 1e$.ri:Z 6.~!f '7ss;:1 *soo~69 -0;1aes IC-40. S49o 4.82 as1~s stt1:36 o.oz.es o.fi* lCsb 891/re 4.~ ais.12 *004.es o.'1492. 0;7 ii: 0.6 c g_., Cl~ ~ 0!4*, O,~ a.a o.~ 0.0 0o=;::;..,.._,.....-,....,..,....-."-......~~~ D 1500 Dose* mgit. 0;7.,.,...------------------~ 14aa. *q.os 1ev:el o.S of signll!cam:e =-f0.4 . ~ C) . ~Q,3 c ..~ (1.:2: !);1 Page 75 of90 Page5 6f6 i ~¥ ** } .J , -- - "'~ .. __...! .J En*lr<>n"""'**ln.,,f"ll~°"IJ\<.. Species: .Pimep/iales promelas BpKCJCR Test Number: l4 Daily Cltemis .. Anal' st Concentration Parameter pH (S1J,) DO(mg!L) Conductivity l mhoslcmi DO(mg/L) 300 mg KCIIL Conductivity { *mhos/cm} *Temperature ("C) . pH (S.U~) DO (mg/L) 450 mg KCl/L Conductivity CP.mhos/cm) *Temperature (0C) .PH(S~U.) DO(inglL} 600 mg KCllL Conductivity lOS/cni) *Temperature ("C} pl!(S.U.} DO (ing/I..} 750 mg KCl/L .Conductivity {µmhosfcm) *Temperature {?C) pH(S.U.} DO(mg{L}._ 900 mg I<Cl/L conductivity ( mhos/Cirl) "Temperature {"C) .pH(S.U,} DO (mg/L) 1050 mg KCl/L Conductivity Otmhosfclri) * *Temperature (°C) "'Temperatures performed at the time of !<:st initi:ition, rene\v.tl .or tcmiinati911 by the analyst ideniified in tlu::D11ily Rcnew11! info~atirm table loCl!led im P~e I. ,....;,...:, ity and hardness performed by !he,arialyst idcnlitied,on d~e bend! sheet sp;:clficfor each aoalysis,and'transcn'bcd to this*beneh ,shect.Q:y; *~ 11:.v1 ..... 1rv ttt~!*Vf:. r..*"4rµ Page 76 of90 SOPADl -ExhibitAT2Li, revisip11 ll*01-l4 Cl ':> Page 6 of6 Q' ;;.. * ,,._....,,.'"': i} 5~;~~i Speeres: Pimef!}ui~ proifzelas PpKCJCR t~Nurnber: 14 Day * (J\nalys~ideni,ified't'or each day~ perfonned pH, .D.O. aild conductivity m¢aiiurcments only, i Concentration . * ~an,lpieter . p}l.{S;tJ,). QO.{mgtL) ~11du'cJivity f mhos/cni). CONTROL "*Alkaltoi!Y .<m CacO:ilL> 'l!IJa~dl:I~ {moCaCoJIL) *temp~atu~ C'C) rm (mg/Ll 7.9 300 mg l<:Cl/L .. -Condn~iivity ti'i_. 7*1*: L*~'~=m=hoS'/,=*~cm~)~;~--1~D::__..:...._..,.. *'f~perature (°C) DO(mg/LJ 450mgKCllL Conductivity (pJTihostcm} *Temperature; ("C) pa (S,l!,) . . D.O (mg{j:.l 600 rngl<.Cl/L Conductivity mhostcm *, .;Tcmpl9'ature .*<aC} p.l,J{S.µ'.) .DQ (mg/(.). 750 mg KCIJL. , Conductivity ~=*~ho~~~cm~):..._~-'-1-.;;;..;...-'-"'---' "'Temperature . (°C) ,pH(S;O.) . PO{mg/L) 900 mgKCl!L ConductlVity cJimliOsl.em) * *Tempe~ture t"C) .Pll(S.U;} J)(){mgli..) 1050 rngKCJ/L C<>nducfulity c rtiiiWcm> *Te~perature <°Ci *.. *. . foifial . .Final Jnitial Final Final lnitial Final ltu!>>Ot~t . . , .. . . . ... . .. , ; . ~~~ turespeifcrm~.at*lhe time'ofiestinitia~io11, ~newril or t¢fminatiori ~Y th~_analyst. identified !"'!he: Qaily_~~ewal l~fonnaiion table >;.llru!ll!fft!i and*hardn~ perfonned by the analyst 1de11td'icq 0111he bench sheet *specdic for each :malysis:and transcnbcd co*thtS bcnch sheet by: locdt on Page I. ~ Page 77of90 . SdPA121-ExhibitAT21.l, revision'll-01-14 'Ceriodaplinia dubia: Chronic Refet*epce Toxicant- Control Chart ~. Environmental Testing SolQtions,.tru:. .... '1 1.14 USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1.10 l.08 *******-..****~***.~111._****** .. ********~~-***************:**o*Ci.*~**********~*~*******-.****~*~090* .. ,. *. . ~********-"****** 1.06 *' . . *. . . . . ....... o**.****ai*a.~~***o*******,.*****.*****:*.~*11***..-~** *********.************~********~~,.****ca*.*. .. .. . . l.04 1.02 -z u = 2.5 2.0 USEPA Warning and Control Liniit$ (751h and 9o'17 Perc_entile Cr's) u ...:l CID in l"4 1.5 1.0 * * * .... ~ . ~* ..... * Ii e ~ ~ o= 0.5 - -**"-.***-***.-* .-..:...~ -**~.... **-*~-*-*---* .. -**-"*~**-**-**- .. *--*-.:.-*--... *--'***-~:.----~---*- "'O I *.e: .................... *!7 **** ,, ........... it'~.*.~ ** ~-.~ ..................... *,*:--*** 0 ... 4'0**it . . . . . . . . . . . . . . . . . . . . . . . . 1.4 1.3 Lab~ratory Warningct.ndControl Li1'i1its*(I01#ahd2S~PercentJle.CVs) ........................... **~*-:*~. * : * * * * Qll(f(J * * * * * * . * , . " . . . . . . . . . . . . ......... ti;******,*~** *~*!"*****~******i.~*** ~.*:~* l.2 - _ . , _ _ _ _ _ ~_,;.,._,;,~~.--::. ... : . - -**- *-*-'-~~-***-***-- .. ~-*-**- ___ , , _ * ; - - ... ~**1-***._.:_.,;;_ * - 1.1 * * * ......,~ * . ......,, * :- *. * ......... =- . .* * .............. ,*** .*.-==.* ** 1.0 0.9 7-day lC25 = 25% inhibition concentr~tion~ An estimation. or the conc~ntratfon of'sodimn :chloride* that would cause a 25% re(luctionin C?riodftPhnia reproduction for the1testpopuiatfott Central Tendency (meaii IC2s:) Warning Limits {mean IC25 +/- SA.JO or SA75) Control Limi~s (mean IC25 +/-SA.ls* SA.'90, or2 S~aµ.daroDeviatio~) Page 78 of90 Cer(odaphnfC'I d.ubla Cbroni~. Reference, Toxic~nfControl Chatt State. and US EPA to_fiotatary *laboratory USEPA* \iSE~A 7*dilV \Ch (f: s control. limit~ SA<*** Watillng liin1rs cantro1 't1mns WarilfnaUmlt~ .s._ro c~ritrillUmlt~ 91 f8/~ No~ll (g/l NaCl! tT*:2S CH2S er' s~.*~ . ~:+ s~;o cr ,s~u ct+ $,1.ii <;T*SA:n cr+s,.,,1 cr~s.,..., CT+s~,_. 05*03*1!'1 1.05 l.05 a.01 1;04 l.08 ll.O!l. o.iJil *US a.ill D.88 i.24:* IMS D.$9 l,.5'4 o.66 o.40 1:72 0.01 2 04*07*1S ~.OG' 1,(Jri o.oi 1.04 1.0ii 0;09 ti.!i~* p:r; 0:11! 0.88 i.i4 0.48 D.59* 1.54 o.e!i MO 1.7i o:oi 3 4 ilS*OHS OS.OMS 1.0.1* l.08 1.05 0:01 *o,Ol. i.05 i,oa* n.o~ 0.98' i;t5 o.i~ o.8~ l.i!i o:-ta o.sii 1.54 0:66 o:lio: 1.72. 6,01 J..05 i;o4- 1.011. 0,09; 0.98 1.JS 0.18 Q.88 l.is .D:4ii* Cl.5!! 1.?4 0.66 9Ao, Pl 0.01 5 . 6* ON<\*lS l,~6 i.07 *0.01 i.os. ~-QI!' D.il9.* o.911 1-.1~ *0.1.s :o;as 1.2:!;' ~.48 o.s9 1. ~4 0,65 iMO.- .l.73 0.01 08-114*.iS !;oli. i.01 .0.01 1.05 1.oil 0;09. :~.98 1.l~* 0:18 0.118 .l,.l!i' 0.48 o:sg l;SS. 0.66; 0,40' l.73 oJli 1 011.iS*lS 1.1i*1 1'07 0.\)1 1.Qa ii.og 1.0S 0*!18* l.J:S. 0.18. 0.88 l.25 OA!! Q.59 l,~s o,6$ Ml i;73 om: 8 !J* l0'06'1S 11'!)3-15 1;06 l.07 '0.01 l.OS' 1.08 MB o:o9 o.!ia us *o,1s 0.88 1.25 o;4s P,59 o,sg 1.54, 0,66 o..;o: 1;n. 0;01 -10 12,98-15 1.07 J.01 1;07 1.07 0.01 1;05 l*Oil* D.98 l.1$ D.18 o.89 1.25 0.48 us o:&Ei g.4l 1.7-3 O.Dl 0;1)1 1.05 1.08 o.o~ 0.98 US* 0.18 0.89 1.25 !Mli 0.59 1;s!i 0:66 d.41 1.73 Mi -11 Dl*i,2*16 1.06. l.01 Q.Ol l.OS 1,08 0.09 o:~a 1:15 0.111 0~89 l.'l5 '0;49 *o,s9 1.s.s C).66 ().41 t73 O.o1 12 02*09*16 1.06 1.07; O.ot 1.Q!\ 1;09' D*.o~. 0.9.ll n.18 1,15: 0.89 1.25 0.48 o.s9 l;SS 0.66 0,41- 1.73* O.Ol 13 oi-ofa-.1& 1,06 i.07* o.~1 l~os l.OH 0.09 9'.9!1 us 0;18 U,89 l.25, 0;48 iJ.sg 1.s!f 9,~6 9.41 I.73 Q.01 14 Q~*OB-16 1.0.1 i.07 M1 l.OS 1.08 0~09 ().911 1.15, o.1s Q.~9 1.2$ G.48. o.S9 1.ss o.66 0.'41 i:J.3 (l.O~ 15 o4.os-1a 1;07 1.01 o.(lt., l.OS :l.08 0;~9 0.98 l.iS. 0.18 0.88 1.25 Q.48 0.59 1.55 O,Gli .{J.4t l;'ll 0.01 16 U4*llS*16 t.ciG 1.07 (teil 1.05 J:.()8 0;09 0;98 L1,s 0.18 0.88 1,25; 0;48 o;s9 1.55 o.~& 0:40 1.73' t'l.01 17 *QH0 1;06 1.07 0.01 i.DS 1.08 li.il9 li.98 1,i5 0;111 0.$8 l.25 0'48 o.~9 1.54. o.&6 OAO l.73 0.01 18 b6'07-16 1.05 1:0& a:Ql 1.0S foe 0,09 0.98 1.lS o.111 ().88 l.24. 0;48 .0,59* 1.54 0.66 o.~o l.72 0..01 l!l DHHG 1;06 1;06 0.-01 t.9$ l;OQ, 0.09 0.98 *1.1s 0:1s 0.88 U4 0.48 o. ~9 1.54 o.~ 0,40. t.n 0.01 20 08*0H6 1.07 l;06 O.Di- t.05 i:os l).i>ll D,98 US 0.18 0.88 1.24 o.48 o;S9 U4 0.6& MO. 1;72 0;01 Nille: 1-d It;,;" 7-da~ 259' lnhl!11tlontonc1nti~t1011. An*e&timat.lon of th~ concentration of sodium chlorida that would c;usc *;, *2s%*roductlon In c:~nciilaphniii teprodui:tion for' th& te~l'pbjmlatlon*. CT~ tenttal tendency (mean 11;,,j; $'" Standard de~iation or tile lc~J:wfue&. ,.... , ~a!J,orator/Control and Warnln11 tlmlts . . . .. . .. .._, . . . . ,.,_,. .. _ . * . Laboratory .control-and. Woinlng limits wete es1abl11hed U$inil the standard deviation of.lhe lCn value~ corrospondln8 to the lOtli and ::iSth percentile cv~. These *~*n"guare mor~ shingenttiiii~ ihq.c~nirol end wzirp;~g-llmfls recon\lllended.by*ussM far the test ineiho'd Md 110dp0Jn1; ** s..,. = Staliililrd d.O~lalil)ncorcespond!ng.to tho io** percerit!Je.i;:V,, (s.,,Q =o.oli)' s*.11 = stondard deilia1ioncorrespo11dlng.ta thn 25" l'erceritilo CV. (S,.n 0.111 * = USEPA Control and. war11Jna Limits s....,.: $timdar<f rlevtaticin correspondfng,to the 75** iiercentilc CV. (s,..,," o.<ISJ SMo"' Standard de~l~tlon corre1pondlng .to the 90'" pe1ceritil~ CV. (Sua" 0.62) r:*l= Coeffcclent of 1r.11iat1on of tile IC15 values. IJSEPA, 2000. Um!~ntandlng and Ateauntlng For Method VallilblJlty In Whole Elfluu.nl To~ictty Applications Undet the Nat101ml Pollut*nt Oischa*B" Elimlngtlon Program,. EPA*ll33*1\*00-0IJ3. vs fnv1ronmenlal Prottctlo11 Aganty, i:lncinnatt,.OH, ,,1..-- 'f :;* Ceriodaplinia dubia, 'Chroni~ Reference Toxicant Contttil Chart EnlllronmentaJTeStlng Solutioni;. Inc. Preci&.ion of Endpoint Measurements 40 .=: -e0 c:: CJ =e ~ ~ 35 30 *. ' ~ . . . . . . . . . . . . . . . -~,, . . . . .,~...... 0 *'!*..-**'* ....... !'***-****~ ":-*~. Q. ~ ~ bf) c:: 25 ~ 0 c ._, *r: ~ Q. 0 20 0 u 15 **--*-,~---~*--..,,-~,*-~~-"'**-~"-***-~*--.;..<_,*,.-~----*~-***--.~-*~ . -*.,,-.. . - ... . -..:.-~ '-,."'"":--~"-*' Minimum A~eptance Criteria(> iS.O offspting,per stirViving femaie) $. c:: c ..... c 40 **-*---~-**-***-~*..-*"!'*-,,~._.._ . . ,. __,;-.,.*----;***-.. North Carolina .AcceptaneetJmit ( <-40~0%) d-~*-'*~,--*-"'!'"- . . ';- . ~.:-**~~;*--~;~~-.*:'i'~~:-~*N*-~**:-*>:* >= et! ~ ........ :...= .2 "Cl 0 Q. 30 Kentu<;ky Acceptanee Limit ('S SO.fl%) * ......... -er::c 0 ~ 20 = :: ~ .E:i = 0 ....... u 10 ~ i... 0 er= u 50 . . . _,, _______ ., __ .~,. usei>A u;pe;PMso ~o~~d <901 ~~~~t,~~<41~0%) ,,-,,_,~,~ .. -.,.~,,--,- b 40 '!- 30 *10 Control Reproduc~ion, C6effid~Jit ofVariatic>n (CV), or Perc~nt }Minjmuni :a~gnific~nt,D~fference (PMSD) PMSD is- the minimumsignificantdiffer~mce 9.etvre,en the c$;Jptr9] an.d treatmeD,t that can be declared statistically significant. * .u. Central Tendency (meaJi ControlReproductiotJ:. CV. or PMSO) Control Limits (mean Control Reproduction, CY, or P.MSD z2.StandarifDe~iations) Page 80 of90 Predsion of Endpoint Measurements Ceriodophnia dubia thronic:Refere*nce Toxitant Data. ~if;.1 Tt?St number En\ittoomen~t'Testi~s soiiltlonSj Jn<:. Te$hfate Cont!'Ol suniival .conti'.Oi M~an Reproduction q OJ er MSD PMSD CT for Control Mean for Control (%) {offspritjg/fematel .flt:prOdui;tioh (%) Reptodliction (%) f~rPMSD{%) (offspring/female) .PJI%} l. 03"P3..:1s 10() 3Vi *s.n l.9 5.8 2 04~()7;;.15' loo 32.3 32.4 4.6* s. 3 1.8 5.S S.6 3 05-0s-1s* loQ ,31;7 32,1 5.6 5;4 1.& S.8 5,7 4 06-.09-J.5 10cr 29:7 31.S 2,g 4.8 1.4 4.8 5.5 5 Q7~1.tt-1s 100 30;8 31A :rn 4A 1.5 4.8 5.3 6 os414~1s' 100 ~l.-0 3,l.3 3.0 4;2 1.6. 5.0 5.3 7 09--)5""15 100 2~.4 31.0 4.0 4.2 1.7 5.9 S.4 8 10*06-15 100 29,6 :30.9 4;8 4.2 1.8 . 6.1 5.5 9 :i.1--03~1s lOQ 31.7 3LO f;;S 4.5 2.2 6.9 5.6 10 U-08-15 ,100 ~(t6 3\>.9 4,9 -4.S 1.9 6.3 5.7 11 ch'."12:.15 100 3i2 3o:9 3.3* 4.4 1.4 4.4 5.6 12 02JJ9.-16 100 31.:6 31;0 1.0* 4.6 2.() 6.3 5.6 13 02*P9*1Ei 100 32.0 31.i 5.9 4/7 i.6 5.1 5.6 l4 03~os~16 ioo* 29.4 31_.0 5.4 4$ 1,7 5.9 5.6 15 04-05-16' iQO 29..3. 30;8 :6,q: 4 .. 9 1.8 6.1 5.7 16 04-05~16 100 32.3 30:9 4. 9 4.9 1:1 5.2 5.6 17 OS-10-16 10(} 31.6 *31.0 5.0 4.9 1.6 5.2 5.6 18 06-07-"16 100 2.,7,9 30.8 2.6 4.7 1.7 6.Z S.6 19 .07..,1_2::,l,6* 100* 3?..S 30.9 4.9 4.8 1.7 5.1 5.6 20 08'-02'-16' 100 29.9 30.8. 6;6 4.8 2.1 7.0 5.7 Note: tv: *toeffiCient ofvariatiof! ior controirepr0di.lttion;. L~wer CN bound determined by USEPA (10'h percentile}"'8.9~:; UpJ?ef. t\ibound,deterrnin~'by liSEPA (~ottt percentliel = lli% MS_,<:* MiJUmo'm5ignifu:ant.Iiifferenee PJ1.11SD o::: P'erc¢Jli. Minimum'Si~ifi~rit Diff~reni;e PMsD ls amea*sure oftii$t {'~lcin, The PMSD is tfie minimum percent difference'between the control and treatment that can be det:fared.statisticallv :Sigi-iificant fii a wtioJ£!eff1uent toxicltv*test;. .lower PMSD bouhd.d¢termined by USEPA(1o1h percen'tlle} =13%. . Uppei"l'MSO bound d¢terrriined bv*t.lSEPA(90m percentile) "'47%. q '= *~entra!Tendancy {~eao to~I ~eprodui:tion, CV, or PMSO) VSEPA; >2QOO. Uilderstari~i~g and Acc01.1nting fot'Metllod Variability in Whole Effluent Toxlt:ity AppJicatio!lS: Under the t,iaticioa!Pollutant Discharge us Ellhiiriation Pf0granu EPA-83'3-cR.0()..093, EnVironmeotal Protectiori'Agencv; Cincinnati, OH. USEPA; 2ooia>::zoulb;.1'lnarRepO'rt: !nt~tfabora~ory VarJab!lity study i>fEPA shorHerin dtroni(:.and AcuteWli61e*EfFhsent Toxicity Test Methtids, Vo1t11nes l and 2*A'Ppendi11: EPA*Bii-a-01,004 arid EPA~8:Zi-.8~oi-oos. Os Envkonmerital Protection Agency; Ondrmati; Oli. Page 81 ot90 l 0 0 0 1"-.*-~: P~~e1 of.6 <):*=~~~~ l *~ Sodii.m.Chlotide*Chronic Reference.T.on~nt*Test .!...{EPA-~~AR~o2~013 ~ethod lQ02~9) ~ Spec~: Cedo<i:apluzia d11/iia. Diluti<in preparation nifOTlll(ltitm: .-Comments: NaCl Stock !NSS number: .. *mss lC:::.l~ Stock preparation: 100 gNaCJJL:: . . '*: Dissolve 50 it NaCl m500 mL MUli-0. water. Oilution oi'eo CimzJL) 600.* 800 1000 1200 *1400 stock volume (illL} 9 f2 'lS . :J8 . '"2J Diluent'volume (mL) . 1491.... . .. 1488 148) l482 1479-Total volume{mL) 1500 :1'500* '1$00 1500 15.00. Test orRaiilsm so11rce hi,fi:Jtmtilion: ' 1<!i'til,tformatio1t: Pate and times organisnts \\"erc'brim ~&~(n;'!'\\o. o~o ~ -~ lticµbawr.nwul!crand'$elf between: * * . .. ... loeati9"~ Ctilture board: . i:n,.'\~r \t... .*A ~epJicarc number: . f , l .I . :t, r 4: s r '6* " 7 I *:s.. r !) l .JO. VWTbati;ll: Culture board cup number: . b.. Q h'.'1.. f'l\, Jg I "U I"Jli .11.=i l~"l. ~- Transfervesscl infonna!ion:* *. i:>H= &.o~ ,ti.. Tem!leniture= "'2.$.;~ "C.' ~lilt.as/rum.batch: Average iransfer volume fmL): * : IMl:inL Day . 'tl!St hiifuitijjn and- fe~dfog, MMSW rene\v,i,tl *n.d *feJ:(iing, or* ,f1atcf1.}fscd * *. termin:ition time 5 08.'-0J'-16 I _.I Cotttro/ i11formatiom %.ofMale Adults: % Adults having :;Id Broods:.,; J"" % Mortality: 61.. .LOEC *'* Mean OffSp:ring/Female: <::40;0% J %CV: ! J Page82of90 I l 0 4 ;:I .J:. Page2 of6 l $pec~gs:. *Ceriotlanlttii'ttduhta CdNaCICR#: 191 l *c-ONTR.O'.L S;utvivat t1itiJ lleJlroductiim Datn. *. D.av . :t *. 2 . ;3 . . . 4 5 . 6 .7 8 9 10 l * ** * *1 * .* \'ouilg produi;e~ Adutti!tlirtalit;r CY : * .0. \,...,.' \..... 0 ~ *U L *"- 0 : L 0 0 . L. L 0 C) L :L / t'1 2' Vo'qngprod.uuf) , . 0 :* . () . ** -~-- * h: .* CJ ;0. .D . 0 0 .O -1 Adult mor.tafity . .. \.,..... * \._,., L. L L- *_\;....;. . \..,,.. ., . ,..._.; . *\._ l..._ *s ... *v.ou11Jpro~~ccd * .0 o.. "".* -:""- . f'iif: * . Ci : D:'. *O, .6 0 . . ~tJtdt'mor;talifr-. L -\.-. \....,:.. ~: L; \..- L. .L. .._. '- -1 4* . Y.oungpro~u~: . q-. ,,_r. ' * ~~ . .: 'C\. -~- *4 * *'""\ .* '..\ --~ ':'?\Uuitm~nf.r .*'L. *y L.. *6. * \;,;. L \- L.. L.- \...... ~. *. Yo~ng:erot}~cil . :f{). :, it!:l. ' t\ . H~ \ "t,.. .\,_ i'"t . \1.. l 0 I L5 --1 Adult mortality L.;... ** * \......;.; * \;.....;._, L. L * \...- * (\...... :\....... '-- C ~- * * ¥o~~n~r9thttcq . /:'\: n f'\. , Q () . M . tn* . C 0 0 * .Muttm~rtar,:.ty, 4* : . L,.;._. . \ ..~ . ' 'i-,. :L. * \.+.. . . L . L . '- .. ' - -1 . F'llial:AduJtMonatitV ~ * * ~ L...: '--'* ' - * \....... *i '\L... \..._ L:.c * ' - - I Xfor,3'C!BrotHJS* * '.'~ . ~' . ¥"- * ~ ... ><- No.t~f. Miil.tmo~ity Q,._<=.:nve. o "*:dClid}; SB'::*".splitbtood(smgfe broO!hplit between t\Vocday~), CO =caey over (offspong* * -ca~*~ver:Witl'!~tiltdunngirailsfer): ** * .,. ¥ * *. . X.. . ~-- r... *-,...;;. * Concentiatihn: ~-~%~M~-~orta~-~H~-~;~'--~~"-""T~~~-.--;1 .J Meari .Offs rb1g!Female: ~.'\ R~nlit:i.fe.riuniber . ru. .. 1' .2 .J 4, 5 ..6 1 .8 !) IO J Vp11ng produced~ :. ,6 l'"'\. ' 0 c l\ l'\. Cl. ,. 0 A 0 .... *~iJ~l( l_ll_l)~_t?~.., ..... ... ~. .k '\.- L. .. \.;,_ ,. L L, \ .... L- ... L .2 Y*c#~ng produced ~o. ' 0 D i...o 0 .o Q 0 c. C5 .. Adult . 'L ) .... <<*~ mp~lltr L .. L. L. L -t.... L '- .J Yo~n~ pt1>d1u:cd* *-~ ... r-... f\.. !';'\ <<}. .A . (\ c 0 a A.aw~ nl!li'tal~ L ......L-. *\.._.;:, .L L L :.4 L . L L 4 *Youllg priid~ctir!. .'i. ,q s -~ .. *'4. *. '*'{ H: ~ l,_ \\' ~ J .A,~lt mo!'tlint.r..: .. * .L L. L ;L \....., L.. ! L L ,. : L. L.. . ;; .v:oµng iwoihrced .. l"'l...-. .Jo l.\ Jc;} * : :\ i;,. . .. 11.... *fl \ : 11 Adlitt morfaJify 'L ' L *t...- \;,::... \..... L ' \..:-. L.. ~ L -j 6 ... rouri{'~!'OIIU~li_i!. . f"':l 'h.* .0 .h .A ("\ 0 0 0 0 ! 1;. *Adult'mortaJit,* 'l{o~g;(J~duciid I Jp \... L tS. L '.'S lS' \....,.. L l'"' .tb L _,, L L \'S L l 'S L 1~ -TO.t:t1:youngpr<iduceil- -~"(. 1..1:\. . ~-\ -*' -~' ~~ 'Q\ '2:fL ot 3\ L!( /VD(e,.' J\dtilt m~lit)i Q.. =]ivC,. D -dead)~SB=splitbrom:l {sing!~. brood split betWcerl two d~); CO= c:arry over (offSpring .~ovet\vllhadul~dllrigglt.insfet), * '* * Co11c(mti'atioll: J % Mortaliiv: Mean Offsi:ldng/Female: 01. Page83 of90 S()P ATJ4-ExhibitATi4~1, rc:Vision ll-Oi-14 Species: Ceriodaplinia dubia 800 mg NaCl/L .Survivalali.d Reproditdio.11 J)irtq Day l Youag.produccf **. Q, M. * () I'. ,C). *O * ('\,. 0 * .0' , Pl .Adlllt mortality* \._ (_ i:: *:L. L.,. : . \.:_, ' L C .L ' :*L ~- Yo1111g pr.Od~~d : .' .Q . .. () '(""\ . **./""\* * * "("\, ['i\. *C\ * :Q*. . . :C'\ Ai.lultmortaltiy \_.. ~- \ ...- L L .* ~. \:;.,,..; . L \...,. L 3 Y11ungpr.oduccd ~ * * (, ('\ :f"\ .A* ti '{':\ ** 6* .O l'\. , Adult m0rtalitY L, . '\,....:.. *... L. C "\....... .. L; \...;... :\..::... \..... \;.,.. Young produC:ed Adilltmorlality '°'. * L .. ~- \..- *'4 "-'* \.....,,. * ~- _\.,,.., .. ,,_ . ::S ' *\._ C::::. * ~ . \..... L..: \:...,_.. L --~ * * *q Voungproihiced \~ \L. \j \\ ~ * \1::1 ' \0 \\ ** * *f\, . *:G \\ l Adult mortality. You * 'l l.J.; \ n . o*. . :r*.1 *~ *" - ~- * . .\:.. **L:-, \;..,.. , \..,.... * ' \;;;.,,,,;: :* : \...-_* ** '}.:. C:l . . \'..J Adultmort.lntY L.:. *c * \. ( ~- "' t. .\. I 7 Ymrng prod0cCd: *Tota{yo!ing.produced \ ~ ; l;~. ** * (- *t,p ;2:Q . * ~~ ~*"J.;.. . \~ "L'\ lL.i . : \ ~ '31... l .l . ~'"\ \.;g* ' \ \..\ : 21\ * ~~ .. \lp . l'\..\ 1:.1;* FinaJAdultwtortality . '\ * \..:. L... ~-. L L .~ \;.... ... \;;...;_ : : L. Note: Adul.l!,llOitality (l;=liVe,.D":.'deild)~SB=split~oritf{Sill&le'br.Oi:Jdspti~bctw~n\'oqa~).CO~Cl,llt}'.O~{O.~ carried ovenvillt .adult dUring liiinsfur).. ** . * . .* . .. ' * &,ru:e1ttn1ii0.a: l ~%Mortali .Meifu Offs o em~e: ; *. '()'1~. ~ .. ... . ..;b %:ReductipnftQn:t~tl'ok: : : ~"Z.;$1.. 100() mg N'aO/L Rcnlkntc nilmber Day I 2 Yooogpraduc,cd ,(') * .(\ *** b Adultmorialiiy L L.:. L 3 Youngproduccd .. h () **("\* Adult mortall!Y l.. \,.;;. \_.. *4 Youngproduccd .* i- ****c.J. . --~- *\.:r **: Adult moriali,ty \........ * \.:...;..: ~ ** 5 ll. 1 o .* . J.'.?S i o. '. 10 :. *...tb. *. u . Adult mortlllify *.* L * * *L * *L; .1 6 .o ; .h. * *. G ...* *_ o ,. 0 .. -l1 .., Young produced ,. t'S.: \~ .* I\: Total young produced .* *-i: '-* i ; "\:~ FinalAdultMort:llity . : l~. L.: . ~- * \..._;.. ... '-- * . L '*\;....; *... ~* """- '* \..: Nore: Adlilt mortality (L ""live., D =dead),. SB,.. splidl;ood (s)n8l1!'broo!l sp!itbetweent\vo d;i.ysJ.;CQ-=cal'Q"~iirtoff$priQJl, carried*ovenvitb adultditrlng ltlin~fot). , * * * * ** * ** %.tvfortalDy~ 0l Meafi OftSoriit~Femat~ . ' .* 4&*.1 * *%Reduction 'lfoin .Cpn~IL : ~-~:07~. Page84of90 SQP ATi4' .,,,,µJiil:iit AT~4.;l;;feYei$.ii)n n-:OJ:t 4 l ; O" Page4 of6 1 Sp~tje~ _Ceriollapkniddtlbia . CdNaCJCR #: 191 l l~OO'J~~g:~~f;qL;. . S'i!>'vtvt;i. n1fiilliProilu,ctiOn bat.a . Repli~~te num:be,r Day' f'f '2 1* 3: * . t. 4 J .s I 6 l 1 I. 8. I 9 .I 10 l 1 - r=v~1i11g:p'ioiuc~(f'; :---o.-* A'1ulhnortiiliiY : .* *\...;.. ' ~~o~ L -o cc---o * *o. .. a ... L L \...,. ... ~ a. \.....;,. \..-- . c -~ o \,_. <z r-:y~t.nlii>roii~c¢4 * -h _:. t\- ~5- o-:- -Fi . ~A-~~ -a Ii *o l -~ Adfilt1'1t>rtt11ity * * )'qurig.p~duccd* ** \,;... , b Q. : O. . *O 'L.. L ** D *\._.. ., L 0 *. :(:) : :!'"'\, . A . *L.. .*L. , \.-: 0 \..... 0 1 1 ,'Jid'u~t.modilltty * ~: . L...- ..L . L.. * \,- . " \.,.;i. . ;.1-. -~- * \.....,. : \........ ( i * * .Y~n!qg.iJrc>dU!!ed: .. ~- ~- * *4. ~-* * -~- * -~- .. A* ~ Z "L .~dultm(lmlity L , ~- \......_.. ,\.....; . '..J.... ... . L i*~. \..... 'L. \-.. -I s*~*l~~::::~r~t:J: *t.l*2--Jt.?**r-.tJ *~*-t~ *:i~~: c 1c. ,. . :l"cmngpr.oauia!d' .:D A * *o o ,r:; .. f\,, : *a o. - o * .f"I * .* ~uUJ11or1ttli.i¥ . \._;.. e:** *L,.. \ .....J G L; '- \..... L '- . l 7* _t:tit:ilfPu~priirJu~ -y~u~:prp!lueiid . . .:5 * 1. t~\ J ~ 1 ,.,~l "".L q. '°'.'\ i4J .,4 ~ : * \o . L.\ l t~ :I t o:f i I u. ">~ -~ I .'i *lFiriiil:Adult'Mliriallii~- I Lk ~! L- I L t -.g:**~l:3fI'C I *c.*1 C .r .N014:r Aduft'm!>ftality, :(L-= live, n= ~l. SB'.=split fiioo4 (smglebroi)d:splitbel.\Vc:en.twl5d~;CO=-cariy t)ver(offspi'ing Cltrried' over1"!"th.~i.iu!t 4urtng"'91Sfer}, * * ** * . . . * ** * . <<:onf.iimtfittiQrt:, 1 % Mortaiicy;. Mehri Offspring/f'emate: 01. n.. \ *** :~ lt~d.ui::Pon from Control: Co'l.'\£. I 140.Q.m_gNa;Ol!;, .. *. .$ziruJ1ml"(lnil RejJr(t~ilt:iion /;)Ma Rcnlii:nfe number J>ay l :L 1 J .. 3 I 4 J .5 I- 6 I. 1 J 8 .9 lC) . I f;_J .! 1 . *-~9 pri>d~d~ Youmi . t I ~if':-j.L,J *..**_.-,..,...*.**I ~., "+dult~oft;t~HY,: J L,,.I * 'l:.- 1 ~-' J, L. F\..;,.. J L ..LJ, 'A;.I: U_ *J':> I. t""'J , I. *A. L..> I* ~. * ... . l/.:.J. LL IL .('J L L.. r-. . ... , . ~-* r .. u .... .u ... .,_ -.... , ...... *"*** ......~. ,,,........--~- 1* !"*: *O* * * * .. Wimgpr9ducet1 1 -~ -1**********:r..*-:1** -Q .. ':1~0-*f* **Yo'\ **1: Q* . -(5 a' 0 .. -~~fo~rt?.J~ J:~r~,~-r \..,...-14 l: L J .L,.,.J ::c.1 '- \..... L. *~ Voll~ jirod~~: . 0 _Q_ .Q._ . . ~tlult,mortai<<)'. .. ~ L. \.._. \.....,. *4 * : _ youngprodliccd 0 2 .!L . * ~duTtuiortilli6' .* . L. :L . L L L "S I Y~iingptptl~... * ...*"'.L I .O . .* * ~-- ~- * .. 'L.. . 1 0 '-( l :.u Actu1tm9~tilnry,. *. 'Vo#l1g{J~dlic!lii)~D.r *o: L . L I. 'f) I 0 .~ \,,..,. * ""- . . 4 .Cl J *. *~.- I (). I :n L n \.... \.... .o Aaut(niorbilitYj --c::r-t.. . J 'L -i ~--r~\;......J ~LI ,. L l'L. ~ l._ I '7 1. y-0u~pi;otJncflft l .: .:1-..1. ti :1 .\ . . I. 2> .. L 1... I 6 I ~I -D .t""i 0 .) Tqtrilf.!Jt1~Prd~D-Ccd I 14 J l . J 1- l -~ I ~- *I .'-t I \.\ .I ' "1... 1{ I Fi11aJ.A<JoJtMortali!Y'_c~ ---'--~L C::l \......" r.'L,..;_ :I .'\o.;,. . J . L. 'I '"L. .I'\;- I .L "]-\..... \. ,Nole: AiJjjlt:ri)~i1*l(ty, :cr.*=- li~~E>= tfe!id); sa..:~plit l!_ttiod {single l>fi:\ad.'spli,t betwet:JJ'twodays); C0"" ~flYO\ier (OfJSpriilg .... ~me(lov!?h\litll*a~lt:~,~),, ** * * * * Coiicenlratl<>:'#:. '%Mo~litv: -~ '* Mean Offsptingj'Female: % ReduytiQn frontControl: I . Page 85 of 90 .SOP A7~4-...*EJdiibitATl4~l~ revis'ion 11-0 l-14 ~ 'g Verificatfon.of Ceriod"plt11i<t Reproduction Totals Co C> .) ) () co Control lODO mg* NaCl/L <::> Replicate number. 'ft>liil ncolit'ntc number* 0 II)' Dily Ti1tal I 2. J. 4 5 6 7 8 9 10 . l 2 J .4 5 6 7 8 9 lO 1 , 0 0 *O . 0 0 *, ~O *0 0 * *O .0 .0 ..r ... ' 0 . 0 ' 0 * .O* 0 0 . O* _* o- .0 - O' 0 . 2 *o . O* 0:.' 0 " 0 . 0. *O. . 0 () - .0 0 - 1- *O 0 .. 0 - 0. 0 - 0, -, o. . .. 0 JJ 0 *O J. ..o _ o* .o : o o o _*o o o o . _ o ..... ,3 o- 0. - --* 0 0 0 :O;. 0 0 0 0 0 --~ ..~~ 4- "4 4 _3 5 --* **-* IO .. . lL _ 10: 12. - ,J s - *3 ; 4,. 4 4 ' 3: ' J2 9 .. 12 . IO:'. ...10 - 17 l<l6.. o ... 4.s:* . . . . 4 '_J - i4 5 ,4_ - .. ,:,i* A*_ 3_ 12. IO,- . rn . I ) Hr* *. [3 - JO* . -~10 0 ' o_. .. Qi; o* O* - J. IO ,, 4-38 107 .G. - .0 .. JL' O* . 0 0 0. .0. 0 0 ! 0 -. -:Ii* '** 0 - .o.,- _(I 0 I} . J): 1 . J3 - J7.. rn.. M. 16 1.7 17 U 1.S.. l6 156 7 'IS.. l5- .. 14" 16 J3, Iii l:l 13 .16 l.3 142 *--* - .1*otiil 1.7. Jl. 30 27., 33* J:i 30 .3J 2:9.i ,,29*- 299 - :rotnr. .Jl .28 28 - 32 21 '3.l" 'f).7 :26 ' :2!J 28. " 281- -600; mg-- NaCJlL ,120Q mg NaOJ/L 011y l - .2 .-.J- i *n.cntlcntc*llunlber* $. G -'7* 8 ' !> - ljl.' ; *1'ot,111 . D~y I . *'2** 3. _H.imlii:nte tll1mbilr*' 4 ' -5 6 ' ,, 8. 9*; (@;* T9r1,1.i .1 0 *o** . 0 *o o. o: *o. 0 : **o** o: 'ff t <O . o*. . o* . - -o - 0 . 0 O* - o*. - .0 ' 0 0 2 -.o 0 *O - *._ 0 . . 0 0 o_ 0 *o ir *O .:2. :0 J) , .0 0 .0 . .o. 0 - 0. O' - 0 0 J .Q*' .Q (f - () *. *O . .O* () : . 0 . ....*.o*.**_ *_Jt ...*O ... J Q, 0 *O* ' 0 ' o* .i) o. .. l)* lL 0 0. 4 4 .. 4' ' '5, - '3 4 : ii. 4 5. .3: ... 3 .. - :19 - 'jf :3 ' *3 c2.' .- J. 3* 3 l* '.2', .2; .l -.. 24.... 5 12 . H> *. u. ; 1.0 ' - 12 . 12 . - ll .II.. 13 ll JlS 5* 6. . 4*.** 7 *3 ' 6 .6: .. "5.' ..z; 2 -, 5 46 o* *o:... , JL: ..0 0 0 ::o. . o:: .- U. - -0 :, l} ' -:o* *- 6 7; 0 Ip 15 15 ' 18 ., 11.*: 15 T'7 - lS : lS 'ts' __ JSS v ,, ii. - -o- . .5 . i 0 . : O' - ,0. ..0.' ' 4 "l S. .(f . o* 6'. 4 :* 4* ** 1' . '2 () 4 Total * * . . 3%: 29:'.*, .3:1. :.31 J3 Jt ;-32" 31 *. .:n . *29* 310 1 fohil *14 i 9* 1.3.' 13* 14' *is. lfl . II (i -9 lU . 8P.Q*~gNaCilL 1400 mgNaCllL -*-**- ite1111cl\ic. mil'libcr * - ~------.- Uily 1tttilicnti.i'iii1mlit!r lJ 21*3 - 4> - *S': * - 6; _'7:~ ..: th '9 - ; '10>> Toti1i -Dn)r 1 *2 - 3, 4 s .,,:** . lf. - .9 rn* Tpl11l ' 6' .I. .O.l 0 l 0 *.tJ" '{) *o . '*{), 1 0: 0: tF - '* .Q *" ...'r o* O' - :o- - 0 0 *o:' -*ll'.: . '0: -* - _Q* ...O.* 0 l l o Tc;a-~r.o * -0 : _ :ir*, ' 0: . () '(.! :; - (I - (),. . .::lL... *2 ff". *:o ... -:o. ' ~o . *o . o: 0: .* - 0 l)_ 0 .*.. - () .****'J. ---*-****4 o.J 0 I O', O< ' ll .-. (f. 0. - 0 . ' 0 . - O.: ,() .l . o.. *O *,o**: _o* .0 .. *O .. *.O*. !l - :'O:.: Q *0. rrs:].4: 4 :'3. - :3 .5- ,,A*: - 4, 4, .. 39"' 4 ... - _- *. ::1L* '(l, '_] 2.-. 0 ' L .. ]t j),: '2 0 JC s - 12 J J2J J2 .n. ,J3* lO JI 11 - *13* ' JI- 1'1'.6. .:5* 2 l' 0, . _Q .. 'J. 0 :2. -:r *() .* 4 J3 .o l'it I o _ O* ~ .,. O' . .. o* '() **o i) *o . , .:o *-o --- i'i ... 1 15 *t l:l J 16 J4. *'16 . IS< 'JS 14 Hi.* IA: JS! ;ti... ~1 0 .- ~* - () . Q; ...... 0 . IY * :, '!} __ 3 ; . ':2:* y o-** * ()' 2. i 0* - 'Jl :' o* ()* ;o t tq** 'tutnr*;.rlif r 31lJ .az :* ;2l)'. J2' .28:, - 34, Z9' 33' ',2l>>: ' 3_06 *' *..iro1111. .4 - -.. .1.. :..:.L :5 ~*. *_ 4- T :2 4 Jf' ...........~ /: ~ar ~ Ceriodapl1!tia tit1bH1 Obr9nic P,eferen~e l'oxical)t T~st ltPA-s21,,,R..02~ou, Metliil~looi;o ) ~n'vlronm~ntal testing .Soiutlpns, Inc. V~rification Q.1uilicy Control of l)at? Entry, Caf~ulation$, and Statistical Anlllyses Test 1111mbcl'I . CdN11CICR: #191 Tlist dnfos: A11g11st 02*1!9, '2(116. Ciiliccmracio.11 lh:nlie11tc Jii1mber Sur\'.iv~l .Avcrug_e*rejwqdlii;tbiir* *C<ieflii:ient uf l'C:rciml ~~dimfon lton1 * (II)~) vl1tl~llon ('(.)' c0fl1rol (%)' (mg/I, NnCI) tol'fSpri11glfc11la fo) ? 3. ,f s (;- 7 8 *9. to Coufrol 27 31 30 27 33* .32- 30 31 19 29 100 2M* 6.li Not*appiic11illc .600. 32 29 3l 31 33 3.l 32 3l 31 2\) lQO .Ji.I)' 4.U .J.7 soil 30 :3'0 n 29 32 28 34 29 33 29 ioo 30.6 6*6 -:u HiOO 31 28* 28 32 27 3i 27 26 29 28 100 23;7 7,t) 4,ii 1200 14 9 iJ i3 14 15 ro s 6 *9 IOO IU 27.7 ~2.? 14110 4 *2 .s 5. 4 '4 2 4 IOO )> 48.4 89~3 l>11i111c1t1s'MSO value: 2.JOl MSD .. Mittiini1m Sig11ilic11111 Di£lcrente }'MSQ:.. . -- ~ M .PMSD=. . Pcri:c111Mlriim11m Sig11i!Jcm11.Qiff(lrcncc ..... ,._ ., ____ , .. ,__ ,,_ *. l;MSD is n n'iclistm: of test precision .. Ti1e PMSO is. the 11\inimum perCl!nt di!Tcrencc bclwccn the control. 11nd treauncnt thot. cnn bc*dcclari:d sinlisticnlly significnill inn wlioie cffiuent Loxicity*tt:St. towefpMSDbound dctt:rmincd by USEPA (10°1 perccndlc}"'13%. Upper PMSD bound determined by USEPA (901h percent!ll::)= 47%. Lower and upper PMSD bou.nds we~ dctcmlincd from the 10th and 90th p~rccntllc, n:spi:cti.vely~ of PMSD dnta from EPA's WET lnterlaborntory Variability Study (USEPA, 2.00 Ia; US EPA, 2001 b); USEPA. 200 In. 200Jb. Finni Report: lnterlaborntoiy Vnriohility Study of EPA ShOrt*tcrm Chronic 11nd Acute Whole Effiucnt Toxicity Tes.1 Mcihods, Volumes I* untl 2-Appcndix; EPA-82 l*B*Ol *004 nnd EPA-821-B*O I*005, US 1!11vironmcn111l Protection Agency, Cinoinnu1i, OH. Statistical Analyses *ceriodaphnla**suritlVal*and Reproducti.on* Test-i;teproduction.. Stan Date: s12t.201s Tesno: *CdNaC!CR . .... .. . Sample.ID: REF.;RefTox\canl' End Datil': a19j201 s .LablO; ETS~EnVir. leslin9 .Sol,. Sample Typei ~l-S~ium c;ilio.rl.de Sample Dale: Protoeol: F\iifCl;!R-!;PA-8?1-R,02-01 s Test Species: c;:i;i~cerjadaplloi<> dubia Comments: conc-mg/L 2 3 7 8 9 O"Corit~l *sp,ooo ~gd 27.000* 31.000 *3Q,OOO *21.000 33.IJOO. 32.j)OO 31.0.00 2!:1;00.0* 600 32.000 2e-.ooo 31.000. 31.000 33,000 31.000 32,00ll 31.00P 31;000 800 30;000 :ao:ooo 32.000 29;000 32,ooo 28.000 34;000' ,zs;ooo ss~ooo ~.000 1000 .31,000 28.000 28,000 32.000 v.oo.o 31,000 27.000* 2s.ooo is;ooo 28.000 1200 l4.000 9,000 13.000 13:000 '14.-000 1{;\000 10,000 a~oo.n ti;coo . 9.0.00 1400 4.-000 1.. QOO **+oao !;:oop ~.0.00 4.Qoo i;;OOil'., 1.000 *2,000 4.QOO. Transform: Untransf'arined* 1'-Tailed ISOtimie Coni:-mg/L Mean* N;Meail Mean *Min, Max. CV% N t~Stat Crit<<:a:1 Msc Mean * * ..N~Mean* D-Control *29:900 *1,0000* 29.900 ,;mooo .33,000* s.;Sas Hl .30.500 '1J>OOO 600 31.000 1J~368 ~1.000 2s:oo.o 33.0.0(l' -04,023 1'0 -1.197 2,2a1 2.101 ,~o.sqo. {oaoo 800 30.600 1,0234 30,600 2~0!)0. 34~.ooo 6.572 10 ~i);762 22a7 2:H>1 *30.500. ,,,001)0 1000 2a:wo 0,9599 28.700 2s.ooo ~.(loo ti,978 10 1;300: 2.287 ,z;jj)1 :2a:'700 O;S4,1Q *1200 11.iOO 0,3112: '.11.100 6;000 1s;ooo *21.s10 10 20:41>3 2.2a7 :2'101 i~cicio Q.3639 *1400 3.200 0.1070 '.32110. *1~000 *s;qaa* 48.412 10 29;(!62. 2,287 2~101 **~-- a.200 ~J.1049 - . Auxillary Tests statiStlC .Cl'itit:al Skew* *Kiln l<oJmogorov D Tesl'indic;ltEis norl'llal dlstrjblltion: (p.> 0,01) 0~926.4 't.Q35* *Q;064G -CJ..S:499 Bartlett's Test indicates eoual .vaciMces {p ,,,.0.15)' *a;o1111, 1s:ci$sa Hypothesis Test ('Mall, 0.05) NO~ ....'LOEC CliV TU. MSDu* .MSOp. .MSB. .. MSE F..Prob* df. Donnell's Tes! 1000 1200 1D1:J5.45 2:10oe4: *0;!>7926 1466;94: 4,~Q3;<* '9,3E,40 J?,Sf Treat1'11e11tsNs D'COlltrol Linear Jnterf)o!atiQil*@lf! Resal'!lP!eS), Point .mgfL so . ... 95% 'CL .. ..Skew '!COS 969.444 46.6e<>S '857;991 . 11)10;6 .,0.6536 IC10 1014.2 8.37526 9~!Ul45 1027.~ .!!.~544 IC15 . 1031.53 s,57571 101e.s9 1o#.2s: o.osso .f.0~-'-'-----.....,.--,-,,.-, IC20 1048.8'5 S.1'725 1036,55 1060..77 0.1788 Q.1l IC25 1013!3.19 s;s422s 1os<1.1s 1ons1 0:2398 IC40 1.11.S.18 6,40685 1104.94 11?1.,2 0.2308 o.e* IC50 1152.84 7.5251 t13'7.93 1169 0:1919 o.7 ...... o,s:. g o.s i., . oA a; o.-3 0.2 Ct! o.o ,.6........,...*._,,,._______.o.-"--<6' -0;( >jo..,,_,,-.-_,___._......,.,_.......,........--1 500 t~O 1,500. ooserogfL nose-Response Plot 40 . . . - - - - _ , , . , . - - - - , - - - - - - . , . - - - - - - . . , . , 301.-~~--t--~-._,...l/->-.~- 1.ftiill; o.os tevet ,g.,. 25 of:Si~cafirie. ,s:20 e* *c. ./}. 15 10 Page 88 of90 l g r: Page 5 of6 ~~d2 l l CdNaCICR #: 191 l l Concentratfon :Nranieter Analyst pH(S.U.) l I>Otmg!L) ColJ()uctiVity µmhos/cm) CONTROL '*AlkalinitY ( CaC0;1L) *Hardness ~m" CaC03'L) 600 nig NaCl/L $00.mg ~clJL Conducllvity { mhos/cm) .:"Temperature, (°C} pH(S.U.} .1000.mgNaCtlL ' GoniluctMty l ( hosicm} . . ~emperatur~ ("C) pH(S,U.) _I x200 mgJ'faCllL *Conductivity fttmhos/cml .. *Temperature ("c) pH ($.U.) I necmg(L) 1400 mg Na.CUL . ConductiVity I os/cm} *'femperature.("C) . '*1'¢m~Ul~ Jl'.llrf;oi'riled at the time ofti:St. foiiiatipn, renewal. nr lerininiitfo1t by lhc analyst i_deiitiried m the Daily Renewal InrOiJJUl.ti~n ;rabklocat..cd on Pa~ L Alk.*alinity anritess perfom1ed by the anrifysl identified on 1be beitch sheet specific for each analysis and in'Jnsctillcd:to this !Jencltsheet:by: *. , Page 89 of90 SOPATl4--EXhibitATl4.1, revision i 1-01-14 -i "PT~ ~ ;fik:J r: .~;:.. )W.~l:.~:*;__ l -1 Species; Ceriodap/mia dubiii CdNaClC!t#: 191 1------------------------------------- {Anal !St.identified foreiich da 11-----------...------------ Concentration Pa rameter .Analyst pH{~.U.J l DO(ll'ig/L) Conductivity ( mhos/cm) l CONTROL Albli.iJity {mo CaC03/L) '"Hardn~ 1 (m° CaCO.i/L) *Temperature l .___________ _...f_C~)__.....,___.,,.._.......,.......---~.._..__........._.~.........,_......,_.__.._11----.._..--........._o!i--~.....,_.,, pH(S.U.) DO {aiglL) 600 mg NaCJ/L Conductivity *mhoslcm). DO (mg/L} 7.g 800 mgNaCUL Conductivity }830 C hos/cm) *Temperature (°C) pH (S,U.} 7.fO . .1 no (mg/L) . '7."l 1000 mg NaCJ/L Conduc~ivity . "'I .J**.{) "'I. { mhoslcm) ""et .~~mperature * "1>\.<o . l Jo------+:.pff~.-(s-;u-.J--+7!!:1!'.... DO (mg/L) i~f:>--u-~~~1-~....,.-u-~~~~~~+~~~1---""'lt-!----1 9~ 0 . 1400 mg NaCllL Coqdoclivity ( mhos/cm) S>. 6i 6 .I ~~.e 'l,~ ..lq ~1..\.1 1.'1-"1 Initial Final 'Firial Initial ***:ii'inai hdtiaJ Final *Temperatures perfonned at.th~ tirm::0f 1t:st* initiation, renewal ortenninatiQo by the aMI;iSlide!ltiliC,!:in the Daily'. Ren~ lnfo~ation lab!e lo::ated on ~age .I, Al.klil. i~ity antrdn'e$spedonned b:Y lite analystiilelitified on*ihe.bencb~heet~.fic foreacb anal}'SIS .and transcnhi:d to tins heilch sheet by: . * Page 90 of90 SOPATl4-E;,chibitAT14.l,t~visionJ1*0l~i4 . *. . *- ,, ,*- :*,}}

ML16287A416

Contents

Text

Dear Ms. Hall:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

SUMMARY

Navigation menu

ML16287A416

Text

Dear Ms. Hall:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

SUMMARY

Navigation menu

Search