Text

Submittal of Discharge Monitoring Report for September 2016
	ML16287A416
Person / Time
Site:	Sequoyah
Issue date:	10/10/2016
From:	Pratt P; Tennessee Valley Authority
To:	Hall A; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Resources
References
	Download: ML16287A416 (97)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 October 10, 2016 Ms. Angela Hall Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11th Floor Nashville, Tennessee 37243

Dear Ms. Hall:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR September 2016 Enclosed is the September 2016 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period. The Toxicity Report for sampling conducted July 31 -August 5 is enclosed. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714.

I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.

reston P. Pratt (Acting) Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures):

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name_~~-SEQUOYA~UCLEA~LANT ___ _

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMR)

MAJOR (SUBR 01)

F - FINAL Form Approved.

OMB No. 2040-0004 AddreSL _E. ~BO><.lQOO -

___ _(lNTEROFFICE OPS-SN-SQM) _______ _

TN0026450 101 G

~ODDY *DAISY._I.N_l738L_ _______ _

PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE EFFLUENT FacJilly_..JYA *.filOUOYAH NUCLEAR PLANT_ -

Locatio.!!_ _!:!AMIL TOJiCOUNTY.... _________ _

ATIN:Millicent Garland PARAMETER

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QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM TEMPERATURE, WATER DEG.

SAMPLE CENTIGRADE MEASUREMENT 00010 1

0 PERMIT EFFLUENT GROSS REQUIREMENT TEMPERATURE, WATER DEG.

SAMPLE CENTIGRADE MEASUREMENT 00010 z

0 PERMIT

- 1111r INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. &

SAMPLE UPSTRM DEG.C MEASUREMENT 00016 1

s PERMIT EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE

/g11 03 TREATMENT PLANT MEASUREMENT 50050 1

0 PERMIT Req. Mon.

MGD EFFLUENT GROSS REQUIREMENT DAILY MAX FLOW, IN CONDUIT OR THRU SAMPLE 1807 TREATMENT PLANT MEASUREMENT 03 50050 1

0 PERMIT Req. Mon.

MGD EFFLUENT GROSS VALUE REQUIREMENT MO AVG CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 50060 1

0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE - C, RATE OF SAMPLE 0.1 62 CHANGE MEASUREMENT 82234 1

0 PERMIT 2.0 DEG EFFLUENT GROSS REQUIREMENT QAILYMX C/HR NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified Preston P. Pratt personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, DAY 30

- - NO DISCHARGE D...

NOTE: Read instructions before completini:i this form.

QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS 37.4 0

30 / 30 RCORDR 04 Req. Mon.

DEG. C.

CONTI CALCTD DAILY MAX NUOUS 29.4 0

30 / 30 MODELO 04 30.5 DEG. C.

CONTI CALCTD DAILY MX NUOUS 2.1 0

30 / 30 CALCTD 04 3.0 DEG. C.

CONTI CALCTD DAILYMX NUOUS 0

30 / 30 RCORDR CONTI RCORDR NUOUS 0

30 / 30 CALCTD 03 MGD CONTI CALCTD NUOUS 0.019 0.038 19 0

22 / 30 GRAB 0.1 0.1 MG/L FIVE PER CALCTD MO AVG DAILY MAX WEEK 0

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NUOUS 1/1 Cl(¥ TELEPHONE DATE (Acting) Site Vice President 423 843-7001 16 10 05 (Acting) Site Vi~e President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I

information, including the possibility of fine and imprisonment for knowing violalions.

OFF~ERORAUTHORQEDAGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No closed mode operation. The following injections occurred: Flogard MS 6236 (max calc. was 0.03 mg/L, limit was 0.20 mg/L), Spectrus BD 1500 (max calc. was 0.015 mg/L, limit was 2.0 mg/L).

EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different}

Name _

_!VA-SEQUOYA~UCLEARPLANT ___ _

Add res.§_ __E.Q,_BOX 2000 ___________ _

___ _J!NTEROFFICE OPS-5N-S(llil _______ _

___ ~ODDY - DAISY,_I.N_273BL _______ _

Fac.ill!Y_ JYA -_filQUOYAH NUCLEAR PLANT _____ _

LocatiO.!L _!:!AMIL TOJi.COUNTY_ -

ATIN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (OMR}

I TN0026450 I I 101 T I

l.___P_E_R_M-IT_N_U_M_B_E_R __ I I DISCHARGE NUMBER I I

YEAR I From. 16 MAJOR (SUBR 01)

F - FINAL Form Approved.

OMB No. 2040-0004 BIOMONITORING FOR OUTFALL 101 EFFLUENT

- - NO DISCHARGE D...

NOTE: Read instructions before completinll this form.

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY EX OF 1-------------------+--------.--------.---MA

- X-IM_ U_M _ __, __

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ANALYSIS PARAMETER AVERAGE MAXIMUM UNITS MINIMUM AVERAGE SAMPLE TYPE IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1

0 EFFLUENT GROSS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT Monitoring Not Required 42.8 MINIMUM Monitoring Not Required 42.8 MIMINUM NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER Preston P. Pratt I Certify under penally of law that this document and all allachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the informalion submitled. Based on my inquiry of lhe person or persons who manage the syslem. or !hose persons directly responsible for gathering the information. the information submilled is, to the best of my knowledge and belief. true.

accurate. and complete. I am aware that there are significant penalties for submitting false

~~

(Acting) Site Vice President

Jinformation, including the possibility of fine and imprisonment for knowing violations.

TYPED OR PRINTED OMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here}

oxicity was not sampled in September 2016.

I IPA Form 3320-1 (REV 3/99)

Previous editions may be used (Acting) Site Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 16 10 05 I

AREA I NUMBER CODE YEAR MO DAY Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name _

_!VA~EQUOYA!:!_NUCLEA~LANT ___ _

Addres_L _E.Q,_BOX 2000 ___________ _

_.J.!.NTEROFFICE OPS-5N-S<;llil _______ _

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES)

DISCHARGE MONITORING REPORT (DMRI TN0026450 103 G MAJOR (SUBR 01)

F - FINAL Form Approved.

OMB No. 2040-0004

_§_OD D Y - DA I SYJ~73BL _

PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND FacJ.!i!Y_ JYA - SEQUOYAH NUCLEAR PLANT _____ _

LocatiO.!L....!:!AMIL TOJiCOUNTY_ -

EFFLUENT ATIN:Millicent Garland NO DISCHARGE D...

NOTE: Read instructions before comoletinQ this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.7 8.5 0

5 130 GRAB MEASUREMENT 12 00400 1

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GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE

<5.0

<5.0 0

1/ 30 GRAB 19 MEASUREMENT 00556 1

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- - - - ntr 15.0 20.0 MG/L ONCE/

GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.280 1.306 0

5 130 INST AN 03 TREATMENT PLANT MEASUREMENT 50050 1

0 PERMIT Req. Mon.

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my 1:1;!l:,

Pra,;doot TELEPHONE DATE direction or supervision in accordance with a system designed lo assure that qualified Preston P. Pratt personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering 423 843-7001 16 10 05 the information, the information submitted is, to the best of my knowledge and belief, true, (Acting) Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I

information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

EPA Form 3320-1 (REV 3199)

Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different)

Na~ _

_!VA~EQUOYA~UCLEARPLANT ___ _

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMR)

MAJOR (SUBR 01)

F - FINAL Form Approved.

OMS No. 2040-0004 Addres_L _£'.. ~BOX 2000 ___________ _

___ _flNTEROFFICE OPS-5N-SQ.Nl _______ _

TN0026450

_ _ __§_ODDY - DAISY._!_N_27384_ _______ _

PERMIT NUMBER Facjfily_ JYA -_filQUOYAH NUCLEAR PLANT_ -

Locatio.!!_..JjAMIL TOli_COUNTL _________ _

ATTN:Millicent Garland From

~ 16 110 G DISCHARGE NUMBER QAY 30 RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I xx I ***

I YEAR I NOTE: Read instructions before completinQ this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM TEMPERATURE, WATER DEG.

SAMPLE CENTIGRADE MEASUREMENT 00010 1

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SAMPLE CENTIGRADE MEASUREMENT 00010 z

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SAMPLE UPSTRM DEG.C MEASUREMENT 00016 1

0 PERMIT 5

EFFLUENT GROSS VALUE REQUIREMENT DAILY MX FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 50050 1

0 PERMIT Req. Mon.

MGD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 50060 1

0 PERMIT 0.1 0.1 EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX TEMPERATURE-C, RATE OF SAMPLE 04 CHANGE MEASUREMENT 82234 1

0 PERMIT 2

DEGC EFFLUENT GROSS VALUE REQUIREMENT DAILYMX SAMPLE MEASUREMENT PERMIT REQUIREMENT

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NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments ware prepared under my 1J~1:, Pre*idoot direction or supervision in accordance with a syslam designed to assure that qualified Preston P. Pratt personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submilled is, to lhe best of my knowledge and belief, true, (Acting) Site Vice President accurate, and complete. I am aware that there are significant penallies for submilling false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99)

Previous editions may be used OFFICER OR AUTHORIZED AGENT NO.

FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous CONTIN RCORDR uous 19 MG/L Five per CALCTD Week CONTIN CALCTD uous TELEPHONE DATE 423 843-7001 16 10 05 I

AREA I NUMBER YEAR MO DAY CODE

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name_~VA-SEQU OYA~UCLEARPLANT ___ _

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01)

F - FINAL Form Approved.

OMB No. 2040-0004 Addres_L _E Q._BOX 2000 ___________ _

___ -1J.NTEROFFICE OPS - 5N-SQ~)- ______ _

TN0026450 110 T

---~ODDY - DAISU~736L __ _____ _

PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER EFFLUENT Fac.ill!Y_ JYA -_fil:QUOYAH NUCLEAR PLANT_ -

Locatio.D_.J::!AMIL TOJi.COUNTy_ _________ _

ATTN:Millicent Garland NO DISCHARGE I xx I ***

NOTE: Read instructions before completinQ this form.

PARAMETER C><

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS MAXIMUM IC25 STATRE 7DAY CHR SAMPLE 23 CERIODAPHNIA MEASUREMENT TRP3B 1

0 0

PERMIT

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42.8 PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE 23 PIMEPHALES MEASUREMENT TRP6C 1

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fl NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and alt attachments were prepared under my

~LVJ TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Preston P. Pratt personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering 1~cting ) Site Vice President 423 843-7001 16 10 05 the information, the information submitted is, to the best of my knowledge and belief, true, (Acting) Site Vice President accurate, and complete. I am aware Iha! there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I

information, including the possibility of fine and imprisonment for knowing violations.

TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99)

Previous editions may be used OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY CODE 0'3nO 1 r./ 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name _

__!VA~EQUOYA~UCLEA~LANT ___ _

Addres,L..f.. ~BOJ<.1900 _ _______ _ __ _

___ _(LNTEROFFICE OPS-5N-S(llil _ ___ __ _ _

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ TN0026450 118 G MAJOR (SUBR 01 )

F - FINAL Form Approved.

OMB No. 2040-0004

_ _ _ _§ODDY - DAISY J.N_]7381_ __ ____ _ _

PERMIT NUMBER FacJlj!y_ JYA -_fil:QUOYAH NUCLEAR PLANT _ ____ _

LocatiO..!L _!:!AMIL TO Ji.COUNTY._ __ ___ ____ _

I YEAR I DISCHARGE NUMBER WASTEWATER & STORM WATER EFFLUENT ATTN:Millicent Garland From. 16 PARAMETER QUANTITY OR LOADING UNITS AVERAGE MAXIMUM MINIMUM OXYGEN, DISSOLVED (DO)

SAMPLE MEASUREMENT 00300 1

0 PERMIT 2

EFFLUENT GROSS REQUIREMENT MINIMUM SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 00530 1

0 PERMIT EFFLUENT GROSS REQUIREMENT SOLIDS, SETTLEABLE SAMPLE MEASUREMENT 00545 1

0 PERMIT EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 50050 1

0 PERMIT Req. Mon.

Req. Mon.

MGD EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREM.ENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law thal this documenl and all auachmenls were prepared under my direclion or supervision in accordance with a syslem designed to assure that qualified Preston P. Pratt personnel properly gather and evaluale lhe information submitled. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for galhering lhe informalion, the informalion submilled is, lo lhe besl of my knowledge and belief, lrue, I

QAY 30

- - NO DISCHARGE I xx I ***

NOTE: Read instructions before completinQ this form.

QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS 19 MG/L TWICE/

GRAB WEEK 19 100 MG/L TWICE/

GRAB DAILY MX WEEK 25 1

ML/L ONCE/

GRAB DAILY MX MONTH ONCE/

ESTIMA BATCH

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V1~V~

TELEPHONE DATE Acting) Site Vice President 423 843-7001 16 10 05 (Acting) Site Vice President accurale, and complete. I am aware lhal lhere are significant penallies for submilling false SIGNATURE OF PRINCIPAL EXECUTIVE I

information, including the possibilily of fine and imprisonmenl for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period EPA Form 3320-1 (REV 3/99)

Previous editions may be used P::.n <> 1 r.f 1

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: August 26, 2016 I. Facility I Discharger: Sequoyah Nuclear Plant I TV A

2. County I State: Hamilton I Tennessee

3. NPDES Permit#: 1N0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1.579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. lQIO: 3.491

8. Outfall Tested: 101

9. Dates Sampled: July 31-August 05, 2016

10. Average Flow on Days Sampled (MGD): 1791, 1797, 1793

11. Pertinent Site Conditions: Production I operation data will be provided upon request.

12. Test Dates: August 02-09, 2016

13. Test Type: Sh011-tenn Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids ( Ceriodaphnia dubia)

15. Concentrations Tested(%):

Pimephales promelas: UV treated Outfall IOI: 10.7, 21.4, 42.8, 85.6, 100

UV treated Intake: I 00 Ceriodaphnia dubia: Non-treated Outfall IOI: 10.7, 21.4, 42.8, 85.6, 100 Non-treated Intake: I 00 16.PennitLimitEndpoint(%): Outfall IOI: IC22 =42.8%

17. Test Results: Outfall 101: Pimephales promelas: IC22 > 100%

Ceriodaphnia dubia: IC2s > 100%

Page 1 of90

18. Facility

Contact:

Millicent Garland Phone#: (423) 843-6714

19. Consulting I Testing Lab: Environmental Testing Solutions. Inc.

20. Lab

Contact:

Jim Sumner Phone#: (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone#: (256) 386-2787

22. Notes: Exposures to samples collected July 31-August 05, 2016 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to -intake samples resulted in no significant difference from the controls during this study period.

Page2of90

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 1O1. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY)

(MM-DD-YY)

Arrival Initial (MM-DD-YY)

Sample Time (ET)

Time (ET)

Temp.

TRC*

Time (ET)

ID Collected Received (oC)

(mg/L)

LastUsedBv 07-31-16 0705 to 08-01-16 1307 t

<0.10 08-02-16 0927 101 08-01-16 0605 1.2, 0.8' 08-03-16 0857 07-31-16 0720 to 08-01-16 1307 0.2

<0.10 08-02-16 0927 Intake 08-0l-i6 0620 08-03-16 0857 08-02-16 0705 to 08-03-16 1220 1 4t

<0.10 08-04-16 0906 101 08-03-16 0605 1.2,..

08-05-16 0907 08-02-16 0715 to 08-03-16 1220 1.4

<0.10 08-04-16 0906 Intake 08-03-16 0615 08-05-16 0907

\\

08-06-16 0939 101 08-04-16 0710 to 08-05-16 1235 0.5, 0.7t

<0.10 08-07-16 0935 08-05-16 0610 08-08-16 0853 08-04-16 0725 to 08-06-16 0939 Intake os~o5-16 1235 0.6

<0.10 08-07-16 0935 08-05-16 0625 08-08-16 0853

TRC = Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were wanned to test temperature (25.0 +/- l.0°C) in a warm water bath.

Page3 ot90 Aliquots of Outfall 101 and Intake samples were UV -treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Pimephal'es promelas Test Organisms:

1. Source:

In-house Cultures

2. Age:

< 24-hours old Test Method Summary:

1. Test Conditions:

Static, Renewal

2. Test Duration:

3. Control I Dilution Water: Moderately Hard Svnthetic

4. Number of Replicates:

.1

5. Organisms per Replicate: 1 O

6. *Test Initiation: (Date/Time): 08-02-16 0855 ET

7. Test Termination: (Date/Time): 08-09-16 0848 ET

8. Test Temperature: Outfall 101:

Mean= 24.7°C (24.2 - 25.1°C)

9. Physical I Chemical Ceriodaphnia ciubia In-house Cultures

< 24-hours old

. Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Svnthetic l

08-02-16 0927 ET 08-09-16 083 l ET Mean= 24.8°C (24.6 - 25.2°C)

Measurements:

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Page4 of90 Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville, CA).

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species)

(Type I Duration)

Conducted August 02 - 09. 2016 using effluent from Outfall IO 1.

Test Percent Surviving Solutions (time interval used - days)*

(%Effluent) 1 2

3 4

5

6 7

Control, 100 100 100 100 100 100 100 UV-treated 10.7%

100 100 100 100 100 100 100 21.4%

100 100 100 100 100 100 100 42.8%

100 100 100 100 100 100 100 85.6%

100 100 100 98 98 98 98 100.0%

100 100 100 98 98 98 98 Intake 100 100 100 100 100 100 98

Control, 100*

100 100 100 100 100 100 Non-treated Test Solutions Mean Dry Weight (mg)

(replicate number)

(%-Effluent) 1 2

3 4

Mean

Control, 0.565 0.482 0.548 0:478 0.518 UV-treated 10.7%

0.488 0.461 0.516 0.54~

0.504 21.4%

0.527 0:465 0.430 0:449' 0.468 42.8%

0.481 0.509 0.537 0.528 0.514 85.6%

0.470 0.499 0.431 0.449 0.462 100.0%

0.466 0.502 0.534 0.501 0.501 Intake 0.479 0.511 0.495 0.460 0.486

Control, 0.587 0.555 0.525 0.423 0.523 Non-treated IC2s Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 42.8%

Permit Limit 23 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = 100/ IC2s Page5of90

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species)

(Type I Duration)

Conducted August 02 - 09, 2016 using effluent from Outfall 101.

Percent Surviving Test (time interval used - days)

Solutions 1

2 3

4 5

6 7

(% Effluent)

Control 100 100 100 100 100 100 100 10.7%

100 100 100 100 100 100 100 21.4%

100 100 100 100 100 100 100 42.8%

100 100 100 100 100 100 100 85.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

(% Effluent)

Data (replicate number) 1 2

3 4

5 6

7 8

9 10 Mean Control 31 31 31 34 32 30 31 28 29 29 30.6 10.7%

31 32 32 35 30 33 35 29 33 32 32.2 21.4%

31 32 32 34 32 36 30 31 34 31 32.3 42.8%

34 35 35 31 33 34 32 32

~ 37 30 33.3 85.6%

32 32 34 36 34 36 30 34 30 33 33.1 100.0%

37 35 36 31 36 35 38 37 33 36 35.4 IC2s Value: > 100%

Calculated TU Estimates: < LO TUc*

Permit Limit: 42.8%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC5o: TUc = IOO/IC2s

/

Page 6 of90

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species)

(Type I Duration)

Conducted August 02-09, 2016 using water from Intake Percent Surviving Test (time interval used - days).

Solutions 1

2 3

4 5

6 7

(% Effluent)

Control 100 100 100 100 100

. 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction ( #young/female/7, days)

Data (replicate number) ;

(% Effluent) 1 2

3 4

5 6

7 8

9 10 Mean Control 29 33 31 29 31 28 32 31 31 30 30.5 Intake 35 33 35 32 37 38 32 36 33 34 34.5 IC2s Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/ A Permit Limit: N/ A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = lOO/IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration To xi cant Results OC2s)

Pimephales promelas August 02-09, 2016 0830 7-da~s KCl 0.79 g/L Ceriodaphnia dubia August 02-09, 2016 0915 7-days NaCl 1.07 g/L Page 7 of90

PHVSICAUCHEMICAL SUM M.ARY Water Chemistry Mean Values and Ranges for UV-treated Pimepha/espromelas and Non-treated Ceriodaphnia dubia, Sequoyah Nuclear Phmt (SQN), Efiluent Outfall I OJ and Jntake perform::d Augtmt 02-09, 2016.

Test Sample ID Tempemture (

0 C)

Dissolved Oxv11:en (m!!:IL)

Initial Final Initial Final

Control, 24.7 24.5 7.8 7.6 NurHrl?'utcd 24.6 - 24.8 24.3 - 25.0 7.7 - 7.8 6.4 - 7.9 Control.

24.7 24.5 7.8 1.4 UV-treated 24.6 - 24.8 24.4 - 24.7 7.8 - 8.0 5.5 - 7.9 i

I0.7%

24.8 24.5 7.8 7.4 24.7 - 24.9 24.2 - 24.7 7.8 - 7.9

_5.5 -

7.9 24.8 24.5 7.8 1.4 co 21.4%

~

24.7 - 25.0 24.2 - 24.7 7.8 - 8.0 5.4 - 7.9

~

42.8%

24.8 24.5.

8.0 7.4

.i:

~

24.7 - 25.0 24.3 - 24.7 7.9 - 8.2 5.5 -

7.9

~

85.6%

24.8 24.5 8.0 7.5 24.7 - 25.0 24.4 -. 24.7 7.9 - 8.2 5.5 - 8.0 100%

24.9 24.4 8.0 7.5 24.8 - 25.l 24.2 - 24.7 7.9 - 8.2 5.5 - 8.0 Intake 24.8 24.5 8.0 7.6 24.7 - 25.0 24.4 - 24.6 8.0 - 8.2 6.3 - 8.0

Control, 24.7 24.9 7.8 7.9 Non-treated 24.7 - 24.8 24.6 - 25.0 7.7 - 7.8 7.8 - 8.0

10.7%.

24.8 24.8 7.9 7.9 24.7 - 24.9 24.6 - 25.0 7.8 - 7.9 7.8 - 8.0 24.8 24.8 7.9 7.9 21.4%

~

24.7 - 24.9 24.6 - 24.9 7.8 - 8.0 7.8 - 8.0

.s 24.8 24.8 7.9 7.9 42.8%

~

24.7 - 24.9 24.6 - 25.1 7.8 - 8.0 7.8 - 8.0

-~

85.6%

24.8 24.8 7.9 7.9

~

24.7 - 24.9 24.7 - 24.9 7.8 - 8.0 7.8 - 8.0 100%

24.9 24.9 8.0 8.0 24.8 - 25.0 24.7 - 25.2 7.8 - 8.1 7.9 - 8.J Intake 24.9 24.9 8.1 8.0 24.8 - 25.0 24.6 - 25.2 8.0 - 8.2 7.9 - 8.2

Note: Total residual chlorine was performed on non-treated Outfall IO I and Intake samples.

Overall temperature (

0C)

Pimephales prome/as Ceriodaphnia dubia Average 24.7 24.8 Minimum 24.2 24.6 Maximum 25.1 25.2 pH (S.U.)

Conductance Alkalinity Hardness

Total Residual Initial Final

(µmhos/cm) (mg/L CaC03) (mg/L CaC03)

Chlorine (mg/L) 7.89 7.81 319 60 91 7.73 - 8.09 7.51 - 8.06 308 - 330 59 -

62 88 -

96 7.93 7.78 312 59 89 7.82 - 8.02 7.48 - 7.89 301 - 322 58 -

59 88 -

90 7.94 7.75 304 7.83 - 8.04 7.49 - 7.86 294 -

311 7.95 7.76 293 7.84 - 8.04 7.50 - 7.85 285 - 300 1.95 7.76 271 7.85 - 8.03 7.51 - 7.86 265 - 277 7.97 7.77 226 7.86 - 8.04 7.52 - 7.89 223 - 233 7.99 7.77 209 76 83

<0.10 7.88 - 8.08 7.54 - 7.89 205 - 215 74 -

78 82 -

86

<O.IO -

<0,10 7.99 7.81 205 73 78

<0.10 7.89 - 8.07 7.65 - 7.91 199 - 209 72 -

74 73 -

82

<0.10 -

<0.10 7.89 7.94 319 60 91 7.73 - 8.09 7.86 - 8.03 308 -

330 59 -

62 88 -

96 8.01 7.92 305 7.91 - 8.12 7.85 - 8.02 295 - 313 7.99 7.92 294 7.90 - 8.10 7.85 - 8.01 283 - 304 7.99 7.93 270 7.90 - 8.09 7.86 - 8.03 257 - 277 7.99 7.95 225 7.91 - 8.09 7.89 - 8.04 216 - 235 8.01 7.96 205 78 83

<O.lO 7.92 - 8.11 7.90 - 8.05 197 - 215 76 -

80 82 -

86

<().10 -

<0.10 8.00 7.98 205 73 78

<0.10 7.93 - 8.10 7.92 - 8.08 197 - 211 72 -

74 73 -

82

<0.10 -

<0.10

SUMMARY

I CONCLUSIONS Exposures to samples collected July 31 - August 05, 2016 from Outfall 10 I resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > I 00 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Page9of90

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EP A-82l-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas Samples used in the fatl}ead minnow test were exposed to UV light for two minutes prior to introduction of test organisms. UV treatment is used to control interference of fish pathogens. This treatm~nt method was approved on November 23, 2015 by the State of Tennessee in a letter frdm Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring.

2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

{

2. Ceriodaphnia dubia None Page 10 of90

PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance.lOgbooks.

3. Temperature was measured by SM 2550 B-2000.

4. Dissolved oxygen was measured by SM 4500-0 G-2001.

5. The pH was measured by SM 4500-H+ B-2000.

6. Conductance was measured by SM 2510 B-1997.

7. Alkalinity was measured by SM 2320 B-1997.

8. Total hardness was measured by SM 2340 C-1997.

9. Total residual chlorine was measured by ORION 97-70-1977.

)

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013.. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl.

2.

Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 11 of90

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEP A. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 12of90

Page 13 of90 Sequoyah Nuclear Plant Biomonitoring August 02 - 09, 2016 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016 Date Sodilim Towerbrom PCL-222 PCL-401 Cuprostat-PF H-130M Nalco Hypocblorite mg/L mg/L mg/L mg/L m,g!L,

73551 mg!L TRC Phosphate Copolymer Azole

.Quat mg/L TRC EOIPO 02/06/2005

<0.0042 '

0.028 0.010 02/07/2005

<0.0116 0.028 0.010 02/08/2005

<0.0080 0.028 0.010 02/09/2005 O.Q199

().028 0.010 02/10/2005

<0.0042 0.028 0.010 0211112005 0.0155 0.028 0.010 0610512005 0.0063 06/06/2005 0.0043 06/07/2005 0.0103 06/08/2005 0.0295 0610912005 0.0129 06/l 0/2005.

0.0184 07/17/2005 0.0109 0.026 0.009 07/18/2005 0.0150 0.026 0.009 07/19/2005 0.0163 0.026 0.009 07/20/2005 0.0209 0.026 0.009 07/2112005 0.0242 0Jl26 0.009 07/22/2005 0.0238

.,Q.054

O.Q18 10/30/2005 0.0068 10/31/2005 0.0112 11101/2005 0.0104 11/02/2005 0.0104 l l/03/2005 0.0117 11/04/2005 0.0165 l l/14/2005 0.0274 11/15/2005 0.0256 l 1116/2005 0.0234 11 /17/2005 0.0231 11118/2005 0.0200 l lil 9/2005 0.0116 Page 14 of90 lH50M'"

Date 11112/2006 11/13/2006 11114/2006 11/15/2006 11116/2006 11/17/2006 11126/2006 11/27/2006 11/28/2006 11129/2006 11/30/2006 12/01/2006 05/28/07 05/29/07 05/30/07 05/31/07 06/01107 06/02/07 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04115/08 04/16/08 04/17/08 04/18/08 10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31/08 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016 Page 15 of90 PCL-401 mg/L Copolymer Cuprostat-PF mg/L Azole Nalco 73551 mg/L EOIPO 0.017 0.017 0.017 O.Dl8 H-150M

~

quat

0.037 0.037 0.037 0.037 0.036 0.036 0.036 0.036 0.041.

0.041

0,041 0.041 MSW 101 mgJL Phos hate 0.015 0.015 0.015 0.015 0.015 0.015 0.030 0.030 0.030

Date 02/08/09 01109109 02110109 02111109 02/ 12/09 02/13/09 05/10/09 0511 1/09 05/ 12/09 0511 3109 05/ 14/09 05115109 11 /15/09 11 /16/09 11117/09 11 /1 8/09 11119/09 11 /20/09 05/09/1 0 05/10/1 0 05/ 11110 0511 2110 0511 3/1 0 05il 4/IO Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016

'Sodium Towerbron Hypochlorite mg/L mg/L TRC

'TRC 0.0197 0.0237 0.0104 0.0155 0.0106 0.0129 0.0415 0.0053 0.0049

<0.0141

<0.0160 0.025 0.0152 0.0255 0.0306 0.0204 0.0093 0.0192 0.0055 0.0100 0.0171 0.0041 0.0099 Page 16of90 Cuprostat H-l~OM

-PFmg/L mWt Azole QUat H-150M mg/L Quat 0.0446 0.0396 0.0396 0.0397 I

I

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016 Date 10/31/10 11101/10 11/02/10 11103/10 11/04110 11105110 05/0l/20ll 05/02/2011 05/03/2011 05/04/2011 05/05/2011 r 0510612011 *. '. -

11106/2011 ll/07/20ll 11/08/2011 11/09/20 LL L L/10/2011 11/1 1/201 L 05/06/2012 05/07/2012 05/08/2012 05/09/2012 05/10/2012 05/11/2012 08/12/2012.

08/13/2012 *,

08/14/2012 08/15/2012 08116/2012 08/17/2012 05/12/2013 05/13/2013 05/14/2013 05/15/2013 05/16/2013 05/17/2013 09/15/2013 '*"' **. ~

09116/2013 *.

09/17/2013

~.

09/18/2013 09/19/2013.

09/20/2013 *,; -

Page 17 of90 PCL-401 CL-363 mg/L mg/L Copoly-DMAD.

mer Nalco 73551 mg/L EOIPO 0.036 0.036 SpecttuS. H-150M. MSW CT1300 mg/L d 01 mgJf '

Quat mg/L Quat

. Phos-0.04 0.04.

0.04 0.04 hate

~

Flo guard MS6236 mg/1.

Phosphate 0.029 0.029 0.029 0.029 0.029 0.032 0.064 0.064 0.064 0.032 o.o:u 0.03 0.03 0.03 0.03 O.Q3 0.03

Date 05/04/2014 05/05/2014,:

05/06/2014 05/07/2014 05/08/2014.

05/09/2014 09/07/2014.

09/08/2014 09/09/2014 09/10/2014 09111 /2014 09/12/2014 08/09/2015 08/10/2015 08/11/2015 08/12/2015 08/ J 3/2015 08/14/2015 10/18/2015 10/19/2015 10/20/2015 l 0/21 /2015 10/22/2015 l 0/23/2015.

05/15/2016 05/16/2016 '

05/17/2016 05/18/2016.

05119/2016 05/20/2016 08/01/2016 08/02/2016 08/03/2016 08/04/2016 08/05/2016 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -August 5, 2016 0.0118 0.0112 0.0096 0.0164 0.0235 0.0110 Page 18 of90 Flo guard MS6236 mg/L

'ii* Phosphate 0.03 0.03 0.03

Page 19 of90 Sequoyah Nuclear Plant Biomonitoring August 02 - 09, 2016 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

Pect Name: Sequoyah NP Toxicity P~. Number: N/A F~lity Sampled: Sequoyah NP NPDES Number: TN-0026450 SQN-101-TOX Comp Comp BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of _I_

Environmental Testing Solution, Inc.

3 51 Depot Street.

Delivered By (Circle One):

FedEx UPS Bus Client Asheville, NC 28801 Other (specify):-::OY\\iC.. \\*el ~uer Phone:

Fax:

Collection Date/Time SllUt

~d 11*1,, /{;

o'loS:..

i:::>~o5£t"

'?.J/-/{.,

1 - 1~1 01to<U' /JfP. 20e 828-350-9364 828-350-9368 2(2.Sgal) 1(2.5 gal)

Flow (MOD) l'1<ff>,gJG, Yes x

Rain Event?

(Mark as Appropriate)

tfYcs, No Inch ca

\\.\\3 Trace Sample Custody-Fm in From Top Down Date/Time Reee.ived By (Signature):

ETSLog Number Laboratory Use Arrival Temp.

By Time

Appear*

"C

~

&!ICC

- ~

Tn$tru(:tion$: Clients Should tlll in all areas except those In the "Labora10ry Use" block. Bi<lt11oilitoring samples arc preserved by storing them between 0°C and 6°C and shipping them in ice (samples should never be frpzcn), ihehold time for each $J1llplc is 36 ~ours from the time of eollection. Therefore; please collect and sbip in such a wa~ that the laborlitory will receive. the samples with amjjle time to inltiat'e testing Within that time frame. Samples shipped overnight on Friday via FedEx or UPs must be marked for Saturday delivery or they will not mive until the followirJg Monday.

r Whole Effluent Sample Receipt Log

sample temperetllre performed 111l11C S.mplt Rece. vln1Tn11mometer. $N llOSBOQll~

Date Time Received Received

sample

Project Sample s.ample name and description State C:ommenu Received

~etelved by front temp. 1°c) hUli'll;ler numbet.

011;01-lfi 1013 K. Keenan J. Kennedy

' 4.0 116~6, l6Q801.0I Tu,cl<<tsel1ee WWTP NC 08*0H6.

1307 J. Sumner TVA Courier 1.2/0.8.

1163'7 1:60801.02 TVA - Sequoyah NP -101 TN 08~01-16 1307 J. Sumner TVA Courier 0.2 U6~1 l~B<Jl.03. 'TVA.* $eauovah NP -Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12

aent: TVA

!i>ject Name: Sequoyah NP Toxicity

~*Number: N/A

~ility Sampled: Sequoyah NP NPDES Number: TN0026450 C II dB

. Ma.rcc,;.5 O -.>*?ccrc.. r o ecte y:

SQN-101-TOX Comp Comp Relinquished By (Signature}:

BIOMONITORJNG CHAIN OF CUSTODY RECORD Page 1

of 1

Environmental Testing Solution, Inc.

351 Depot Street.

Phone:

Fax:

Asheville, NC 28801 828-350-9364 828-350-9368 Delivered By (Circle One):

FedEx UPS Bus Client Other (specify): :Son \\<!. JJe \\ i \\J~ t Container Number &

Flow Volume (MGD)

Collection* Date/Time Rain Event?

(Mark as Appropriate) f'1b1~

~ I \\.\\o!l-Laboratory Use Collected

~1--~~~

..... ~--'-~~-+/-::

S1a:11 End Yes lfY~,

No Trace lnch~s

'b*Z>l~

'8 -J - }~

f)PJD5'<tt.~

D&D5r.;

x* o.o3 8 --z~ /&

, ~ ~ 3 *16 0? J $" "£1: 0 Io e.T.

x 1(2.S gal)

Sample Custody"- Fill ln From Top Down Date/Time Received By (Signature):

So.""\\C..

l:.O.\\.<<.tj ETS g

ArrivalTellljl *.

By Number

c l ~f18ba. 2:3 \\.'2. \\A *c... ~

\\. '-l 'L Tlm*e

~'

h.1..()

\\"1.. '1. o er Appear*

BQCC i

Instructions; Clients should fill fn all areau.xcept those in the **Laboratory Use" block. Biomonitoring samples 11rc preserved by sto-ring*thern at 6°C and shipping them in ice. Thehold time fQr each sample

is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefote. please collect and ship in such a way that the labpratory will receive the samples with ample time to Initiate testing within th~t time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked fot Saturday delivery or ihey will not arrive until the following Monday.

Whole. Effluent sample. Receipt Log Date Time'

~ecelv1,1d fl~cf!IVed.

*$llmpia

'Project sam~le Sample name.a11d descriptfi)it

stat~ '

Commehts Received..

. R11~el!ted.

by

.ftdrn:..

remp;(~Cl. numbe~

i:iumb!ae' os~os~:i:G.

'0940, K>Keenan.

ues:

2~6.'

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1;3' 11652*. 160803.* 03.. MoceheadCltv WWTP**

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,Fed.- Eic 1;5 11654

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. 160800.* 09* Jameslougtilrit WWTP NC OS*OlHG 1021;

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' 08~03~16 1021.*

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'.1'60803',21 Carolina Beach WWTP NC QS-03*16

1oss K.Keenan*

J.Kennedv 4.0; 11636 l608Q3.~2. Tuckaselgee WWTP NC 08*03-16

.1220 J;sumner

.TVA*Courler "1:2/1.4 11631 160803* ;23 TVA ~*seauovah. NP

101 TN 08*03;16 1220 J *. sumner TVA courler

1.4

.11'637'

.160803*,24 JVA;-Sequovah.NP' ~*intake

TN 08*0~16

- 1zss.

K. Ke-enan' Dash Courier 0'.3.

11671 160803.25: OWASA NC

O&!l3-16 1500' J:Sumner TVA Courter

.O.S':..

11672:

160803 ;26 TVA* Shawnee fp.,. OOlAM KV-08*03-16*

1500 J.Sumner TVA Courter o;9'...

11672 1'60803,27** TVA~ Shawnee FP'* f ntake AM KY*

I 08*03*16 1500

.J,Sumner TVA Courie(

o.a

. 11672:

160803 ;28 TVA' Sha\\Nnee FP'. 001 PM KV*

08-03-16

... 1500.*

J. Sumner *....

,,. TV.A Cci.urler

1.3 11672

160803'.29.. TVA.. Shawnee rP ~:intake.PM ~.,,,

KY SOP G4 *Exhibit G4.2, revlsion 01-03-12

BIOMONITORING CHAIN OF CUSTODV llECORD Page_l_ of _1_

.. 1 Client: TV A EnvironmentaJ Testing Solution, Inc.

Delivered By (Circle One):

~ Project Name: Sequoyah NP Toxicity Asheville FedEx Location.

FedEx UPS Hus Client

~ P.O. Number: NIA 628 Patton Ave.

Other (specify): Sol!Ji~l.*vuy c

~

AsheviUe, NC 28806 General Comments: {fj z.... r VI Facility Sampled: Sequoyah NP

!Oo~ ~?/~ i~Jt>lti'~eri 071 c

i*

NPDES Number: TN0026450 Phone:

828-350-9364 ht;i:.5(A?1/t'.$ ro~ ~

~ ~µ.,.,-e ()itk lei

~-'!h'l t

~~l'~~~/j~~~

v Fax:

828-350-9368

'./-

\\

£' ~*' "*

~~.,,..

A 0 *

~bt~.5 Mid t..Jet'( ttill~Je~-t J:eJ)I p>-r_J,bt:_

v v

./

P/U)l~ I '~1-F I

Conu1incr Field ldcnlilJcation I GrabiCornp.

Colieciion Dateffimc

'Number&

Flow Rain fiv.cnt?

Sompic Description Volume (MQD) l.aboratary Use Cull1:c11:d (Mork as Apprnprfaic)

Stnn "F.m.1 Yes rrv,s, No Trace F.TS log Aftivol Temp.

'Ry Time-Appear*

~-

Inches Nutnbcr

("Cl

. a-once

<l~'l*ll.P

$-5', I<.,

x lbitJoSIS o.s o:'\\'C. a'

\\~';

SQN*IOl*'rOX Comp 2(2.5gal)

!*193.o~S o.L\\'t.

()'110~ ~-

O(..,.lD~'f I

SQN*llff *TOX

~-1.Ht.,

<6-5~ It.:.

-x *o.t.ft

'"'~'"

().~ t.

\\1.)S co,np 5 I 1(2,5 gaJ) ff}J!I

~

D1Z.5e. DIPZ..

Sample Custody-Fill In ftom Top Down

~ UJs"U>>l>"'I StA\\S llilT'f!(.M. SN-\\J\\e$

Re~quished Sy (Signature):

Da(e/Timc Received by (Signature):

U,U\\ )6)

.~ &Cltlb

\\Q~Drt'l()N. \\~

Oate/Ttme ~

1to) 1\\\\,.1

,_ '.wa: _-.

-~~

/

Y*Gik/t>3 C.OE ~ /J~

((-,, ~9'A~.Of'-o;:./b

-~... p*~"!"' U'

-~~

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./

f:,"

~*

r

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t

~If 9--1 ii..

M**~

CJ y-.. p$'-l&.J/J.~... ~~I*

£~

O(*~*llo t"L~S tr r

'-J lnstructie>ns: Clicms should 1111 in illl arcas except those in the "l.obnratoey Use" bfilck. Binnionitnring sumplcs ore preserved by storing them between 0°C and 6°C and shipping lb.em in ire (snmplcs sJ1ould nllver be fro7.eo). The iiold time ror cuch sample Is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from theJhne of collection. Thercforii, please collect nod ship in such IJ WU)* that the faboratory will receive lhe s:implos with umple lime lo initiutc !csllng within thnt tirnc frame. Sample:t shi11i1cd 1wcmight on Friday via FedR" or UPS must Ile marked for Sowrday delivery or Ibey will not. orrl\\'e until the lbllowlng Monday.

('-.

Whore Effluent Sarnple Receir;t Log

~

Date Time Received CQ C'D

!Jecelved.

Received bV.

Sarnple name and description *

sampi~*

PfoJect ftom

. T~mp. (~O. *numlli!t*

nlimbet Re~el\\l'ed Sample l'I.,)

Oa~05~15

:0946 Kd<'eeoan 01

08*0~16 0946 K.K~enan 0

os~os~1F..... ::.1011.

K.: Keeiien* *.

\\JPS..

0.6.

..11650.... : :.160805 ;OJ: Ror::khighamWWJP:

NC-Fed~ ex.

J:a 11653. <160B'OS,.;03. Belews Creek ss

Ne (0

os~os~1is l011..

K.Keenrin**

Q

.. OS*O!i*l6 1011 K.Ktlenari **

Fed ~ Ex 1.1 11655 160805:.05 D6bsonWTP NC 08-.05-16.

1011

.K;Keenail Fed* !iii

- - a.s 11656

1aosos.~06' Marshiil!Ss

Ne.

08-05*16 101r*

t<l Keenan.

Fed,,ti(.

.o.s 11657_

. *160805,07 North Cary WWTP NC 08*05~16' 1011

. K* Keenini

. F.ed. E'X o.a 11658. -J60BO!F.()&" JarnesfoughllnWWTP..

NC

~

1011 K.Keel'ian 1011

.*~.

os~os~1s**

1011 Fed - t:lf*

1.1 11659 1608US'.09 Performance Fibers

NC

os,os-1.s._

1013,:.

K;Keellan 08*05*16:

1011 It Keenan 08~05~16 1203 K.K~hah H* Bingham 1.3.'

11636;

160805 J4

Tuckaseli;iee WWTP' NC 08-0S~lEi 1235

.... J.,Sumner:

TVA Coufler O,S/0.7

.. 11637 160805'..15: TVA - Seauo\\.iah NP ~101

. ' TN

'08-05-16 1235 J. Sumner*

TVA Coorier D.6 11631 160805, 16 1VA.~ Seq~ovah NP* Intake TN 08-05-16*

1301

J. Sumlier Dash toifrler,

130M **

i:

11s1:r 160805.1 T *. OWPSA ~ Mason i:am1s.

NC SOP G4 - Exhibit G4.2, revision 01-03-12

~~ere

~'c~ q

.ik.:.-,

j,__,~...._....

P~ge. l Qf'l I.

Chronic Whole Effluent'Tr,xiciq T~t (,E!P;.;\\;~21.. ~':-D2~ot31\\fetmjd :to,(l.U}

Species:. PiHtep[zales pro111e~a,s Client: Tennessee. Vallev Auth&r.ity Facility; Sequoyah Nuclear Plant*

NPDES #: TN002645(l Proj~t #:

\\\\I.~"'.\\

Dilution orevarn.tion i1tfQr11.iatf(Jn:

Dilution prep (%,)

10:1 21.4 Effluent volume (ml.}.

'i67~}.

535 Diluen1.voh,m1e.(1nL)

~2.$*

'19~5 42:$

1()70 1430.

Total *volume {ml:.)

2SOO

uoo**

is(}()*'..

Test orea11lsnt}~fonnation:.

Organism source:

In-hctuse culture Age:

< 24-bours old Snawndate:

.* CJ'\\~ t.2'- \\\\:,.

8$:6,

'2140 360.

2500 Hatch dates and tim~:

Ot.*o\\~\\t.. l<Stit>- -re

.roo 2$00 0,

2500 o

(~GL.;\\\\Q..&~

Transfer vessei infonnation; *

.pH?O t*.t>lo:

s.th T¢inperai;ute;?

'\\Sc* \\

oc;,

Average transftir v{)lUme:

,OjhnL Cou)1ty; HamiltOJi Outfall: 101, *

- - .toinnleitis:

. Each oon~~ttaifun;*Wa$ JJV-ttea~

for: 2 minute$ t:0:remove :"Patho.e:enie r.ltterl'erences..

.."f/tt$i inl~'f'J1~ati:01t:

Ratidorilizing teml:>late:, 'Tc-f IJU(Q...

, 1ti~uba1QrI~u~ei~~a I '""\\ t.

-*.shelt'b>ta~lOJl; Ailei11ia,C1tM:number. l CHM882*

})IJifngin/ornltlffemfor 1v~fji#t

. detenN.11tlit1on:.

Date /Time m/oven:

lnitiafiov¢J!.tim'1Pera.l::ilm:: '

~~ d\\a>

\\o.ort

'"1."\\.~'1~

Daily feeding a11d renttw<<l info.rmation:.

Dny D.ate M-Ornin~ *fee.Gin~

Afterno~ feedi*g

-Time 0

08-02-16 Os.-0.3-16 2

08*04-16 3

08-05~16 4

08*06-\\6 5

08-07-16 6

08-08-16 7

08-0~-16 Co111ro/.i11formatioit.: U\\Jf

% Mortalitv:

Averag.e weight per itiitial.iarvae::

Average weight per si.irViving l?.,rvae:

Page 26 of90 Te~ Jniti:ition1 rencw:il, pr*.

teniJinati'o.n S:imp~e nuirillers ~d *.

Masw bat:ch wi~

Tlin~ I.A1u1l~t Qntfa11'.l0l. I h!ta.ke

.1~.4~\\;~. :1¢i~~~1t.:..

\\.1o ottlt-D?> *. Hn~-a.tlo.

'"-'ui(i'b.~:. loa.m..t~~

- itq".11.fm.~'2i!

lt1t'*Aa*11, A

..,ok*fn.-c1c,A bk4n.*L(Dl

,. t*fn.*l~

'l' ".~-,. ;/"" *.....

,,~. _:i;,;.:,::_.,,,.,... <<

7.,;0ay:icso 1:1 NOEc*.

~o.2S!ll.f!llarva¢ 1 *t.O"Et'::

'riv

,.l~

.SQP*AT:20*"-Exbi~t,AT.2.,0:3i.tevi~ion LJ-OJ.,14

Page2of7

Sp~ies: Pimepllnlespro~ins C.Uent: TVAI SOOuoyah.Nuclear.. Piant, Outfall 101;*nv-~tet1:*

l>ate: 08-02-16 StU'J!iilitl trnd Gtowtlz Data CQNTROL.

10.7%

21.4%

,;/\\:... 1\\

c I)

E F

G.

H

l.

.J K

L w

10 10 w

to 10 10 10 IO 10 10 IO ft):

(0

{(j 10 to l*D lO to

{Q {0 10

/{)

lb

/f) to ro LP CQ' lo

<o f O 10

!{)

10 2:

tc, llJ lb 10* 'l() ~b J.()

JD 10 10 10 It)

]Q *lo Jb ro la 16 lb ttl IC

/()

/0 10 JO*

. t D

Hl JO JO

.. lo*

f 0 hJ ID f u 10 10 to l*D ro lO*

IO Jt>.

10 l:b lO lD ro IQ IQ ro re ID. to

.. IC lb ID JQ /0

/Q I'()

C =Larvae weight (ing}.::i B-A.

Hand calculated"

5,.;.'S. c{,tz.. S..tlt '{:ti' Cl.it q,.~\\ ~.J~ S.c.tC\\ S. '.'2:l ~. ~S c.t.~ "i.'-t'\\

wr,yst:...... _____....,1 1\\""*~*""'"*. --.--... _.......

. ~A'I!

(?J;.,'7~.:.

Co'mment tC>i:fd: c = crew-. d =

d,ead,. rg*7 :fi!ngu~ k =

kiU~ m '."". miSslng;.sk ;= si~k, Sill =*unusually 5mall, lg=> uruiSUalJY large, d&r = decat)ted and retumed,,w= wounded, Page 27 of90

SOPAT20-ExhibitAT20.3,revision 11-01-14

Species: Pimeohales pronz:e/as Client: TVA I Seguovah Nuclear Plant_ OutfaU101. UV~trefitedc S111~vlvalmn'f Gi'owl/J. Datil-8S.1io/o p

_(~

-- *a

s T

lQ 10 10 to lO 10 10

[{)

to tef 10 10* ro l

iJOO%

,a*-

V:

\\\\f<

X-IO 10 to 10 lCl lO ro ID 2

i~ (:" - lD. l.0 _ f"_

lO

(

~

y t:)

fQ*.

3 4

6 7

A= Pan weight(mgL._. i_{'

Tray color code:: "\\=1/..,,... ~J 0

Analyst:

ll'w" Date:

1~

.2;.n JV

C = Larvn_e eight (mg)= B..._A Hand ~kulalcd.

Analyst:

Weight per initial numberoflarvac(tlig)

= CI Initial number oflar.vac Hand c:dculated.

iA Analyst:

6\\

Average weight per Percent ~dui:tioit.

initial number of from eontroHrci) larvae (mg) t0 ---10 lO '10,

\\('.)

"\\

lO.

(i)

0)

~

.. 't LQ.

Pt 10*.1:0

.. IO

/O : I 0 * -l Q

( 0 IC - Hl

llf*

'\\t10 159) t'l\\B~ N32-1$,RO: ~

r~-~ Qt.ls-. llo-0. IS-.'A--l1P,£t Js~

t~ii>.

- o-*.cs:*:.

'-.~ri Comment codes; c = cleat, d;: dea~ fg;::; ~gus, k= lcilled, m..: rniSsingf.sk =-.si~k; sm-;:;.ilnu$u~lly small, lg= unusually im-ge, d&n= deca$ted and return~ w= wotinded.

Commeizts.:

Page2Bof90

Page4 of7 sp~ecies:. Pinzephtdes

pronl'etas Crteit: 'TvA/Sequo.yah NuclearPfant,.Outranlot, UV*trHtecf*

Date: 08-02-16

.1()0% Intake Co~ttol-,Nbn-treifim 2.'

3

4 5

.A= Pan weight{m*,.. ~,..,,,,..............,

. T!";iy colono<Je::....._....:;;*,.="'--'...;;.;...._;,.;....;;:_.

. Analyst: -.::,-...,.._~~:.,..-.'n;-___.;.;;..._...,.

Date: _.. --....:!!:Z::.::C!J.LU~--'-

B ='Pan+ L111*\\'ae ~veigbt.(iilg):

Analyst:. 1114 Date:.

~=n~1'il.

C=.'Lllr~eweigllt(ing)""B:'-A.

.Hand.calculated.

Analyst; _________ _._

Weight per inltialnumber or lal'.Vae{mg).

y 10 r.*

... o (Q

/Q ta Jo 10 10 z

u 10 lO (0 lO

l~t) lb ID re.. *.

IO to lo,,()

to tO '

iO lb BB cc QD

.&E l{l lQ IO 10 tp lO IC. IP 10 ro* (Q.' 10 ro IC lC 10 10 to f 0 lo 10 JC JO 16 IO U.)

10 ro.

'\\'

10 io

!O

=c /Initial numherofllimi.e o *

. 'I

' *~.*.L<;*.*

.. ~ '.)t*fk>

.* ~*..

~*!\\,

i.,;9. '>"'"**.

Hand calcu. la*.......

.d1**:*_*....

L.:

u;u

. k*.

.,..~

Ii.~.

Analyst: ------...,.....,.--

o~. tf' ti

\\I 6"

u*

v

Average.\\veig1tt: per

'l'eJ"f.:entm!lldi~n initial numbcr'of from ci:mt:rol *(%)

l:lrvae{m ).

(,.1.7..

"FF lO to f (j Commentcodes~ 'c =clear, d= dead, ~g =fungus; k = kiHed;* rn =missing,.sk =sick;; sm = unusually smal~

lg= unusually'large, d&r= de<:anted and reromed, w = wotind~d.

SOP AT20-Exhibit AT20.3, revision ll-01-14

Environmental Testing Solutions; Inc.

-t'C Cu1tt~til 1.

- no***

t\\lotl*ft1'U"d llt Ff

,\\

CblllrOI,

R l*V*ir<nltd c

I) 10,?%

I'

c*

11*

2M'lli J

K I.

M 421!1%

N*

0

.'I' n

SS.~~*-

R

-s T

u**

100%

\\'

w-*

X.

I' tllb.%lnt~ico

'Z

.,\\A-

.Hll

~

o..Hntll't MSP ""'"~'

l'MSD1 hii*i<*1_

!lun.ttttt't lltSP ~1101 1!~1$1>1, rnt1Wl\\Yl'llil"t.ftf*

Vft111fJt11"1bfrtirJ:art~9 f.ur~~

10 10 10' IU 10 IQ

.1q -

IO

11)

IO I()

to

-Ill IO.

IU.

JO 10 to

-10*

10

'Jtl rn*

JO lU

10 10

'10.

IU 10 10

.JO"

.'Ill JO IO

.rn.

IO to IO-to

IO.

-10

10

- 10.

IO

10.

--Hr JO

,u 10 Ill 10 10 IQ*.

10 10

.9 w*

JO 10 10.*

1(1 10 JQ.

.9 "A;.r:titJ;RU~fi1'1f

~~~l 15.. 14 14.114 lf\\.$7 16.0l**

14.'JO*

IS:~O IS.82 lH?

HAS

-J(i.70 14.92 JG.I&.

*.. u.oo

'1669 16-.02 15.88 14.70' -

IS.SO

~

1~,~

-'IS;45-

16:13 l.1f!7._-

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IS:76-IS.

15.8

. M$Q:*

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II.Ii.

O.Ol!l-I 9,3.

TVA I Sequoyah Nuclear Plant, Outfall IOI August 02-09, 2016 Pi1111!plwle.~prm11el11s Chronic Whole EfOucnt Toxicity Test EPA-821-lt-02-013~ Method 1000.0 Quality Corftrol Vcr.ificnti<in of Onto E11t1'Y, Cnlculallons, and S_t11_tistlcal Aimlrscs No111>1 Conmlionco ""'""n1, l1111!m*l l;ohn1n101y QC n... t'11q + l.u.f\\.'4t udl:lil,(111f;il lnl"l'IW \\t~li:hJ tmi::l

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11.l(I-0.548 l<l..17

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US*

0,488-2tll UI'.

- .~

11504*

lU,08 5,16

..21.67 S,4!l" 20:87' 5.27*

.ll.l*I

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0:468 20.ll

. 4-1()

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-4.4~

M4!.J 19 SI 4:81 OASI..

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OSlS

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0,495

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7.7

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1_16_l'I_

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!}~Gii 0$14 (1;;161..

Q.501 o,136.

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9;7 Q;9 10.8.

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ll,-~,

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lJSEPAc lllUI~; lfJOlb, 1'01o! lt*Pliit! _lotoil.iboi;i1uiy jl'w~llily_S\\111I,;o ofi!PA Shon*l<<m Climniond AcvwWholk ll{lj...,1Toii10Jly1'"1 /\\foll*Olls-.Wlun;.,. I md~*All!f<ndilt.. llP"'Bl_l*D'°l*©t.nd_llP,h*B:u,{Mll~00_$, US lf!\\VltonlJl~lll.t l'!'ll>'.<*i1>1*1\\Utl1C)l.,C:ihl:ion1111;Dll,

TV A /Se<fu~yan t"fu¢1ear flaJ1i1 OutfD:lMOl Au*l!st'<f2~tJ!)-, 2016 Stati$tical Analyses GroWth 16" Eni.foaJe;:

16 Test:!µ:.. f'pFR9R Sample !O:

L\\a:ll.IOj

~,EnVfr.Te'stlri9~

.~1'Yf'~

TVAJ SQN0utfall101 DM!i-Olsciiar91:-Mo71it0ri119 Report pf'.pi1nej:ma1es promeias

.Saf!IJll~ o~e: Aug!,fst 2o1 s

CommerilSf coritr9J-~PN o.sir;;o o.5550*
P<mtrol'-OV o.s!>So 0.48.20
.t-9*1. !l.4880 G'.461.!l 21A o.s270
f!,4650 42;8 0;4S'itf p.5<!~(1
i)5,5. O..f70'i:r lM!lSO
.1ilil o.:ii$6o o:S026
1~*. 1,{47sq
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4 4
4 4
4 4
4 4
0;,941.98 1"52143
0:10128 MSOu 0.05997
...... -* ~
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Z.410 oJ*oa 2:4~0
a,it1cal o;aS4
ts.oses*
... 2.4469'1 MSO o;osoo o.osoc:r o:oeoo O.!JijOO C;(lj'IO!'l.
lsotoQie*
Moan.. H*Mean' D:51Ss 1.0000
.<i.5035 o.971s 0,4908 0~9469 0:4908 0.9469 0;4Sl5 0.!}291 0:4815 O~S.291 0:26917 -1.!)903 MSD MSB MSE f'-Prob
<ff o.nsn o.0022s. 0,00124 o.1ao4s
s. 1a 1.lnoar lnterpolotlon (l!QO~eS..mpfos) wt..1::4EXp!.. SlioW..
t.o -r------------1 0.9 0.8 O:'l
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Q;i' 0.1 Page 31 of90
-..1--r--,...,1.~U:.:O.os1e~
of slgnmcanc~
",,..,,._ ~..,:.~~
{~ **:.>:*, <:>
}
E'nvlron,...nt~I Tostlm; Sotin:.,1nc; TVA I Se.quoy~~ Nnd~r. f!lant, OutfaJI 101 - I~t~ke A~:gust02...,09, *20Ui SWistical. f\\nalyses Larva1*Eish' Gt.oWth* and Survival. Te5t4*Qay Growth.
Start Date:
8121201.6 Test 10: PpFROR Sample lot:;
"NA /<$QN Outfall '101..,; Intake.
Lab 10:
iffl~eovJr.. Testlng Sot.
Sarnpl~ Type:
OMR~o.t$t::tt~t9e. 1\\111),Q~onng Report End Date:
8/9/2016 Sample Date: August2016 Comments:
PretocoJ: '.FWCHR-EPA"821.. a-02.-01a TestSpectes~
PP~Piim:iph~ promelcis Cone-%
Control-NON Controt~uv 10.7 21.4 42.8 85.S 100
!ntake Cone-%
Control-NON Control-UV 10.7 21.4 42.8 85.6' 100 Intake.
1 0.5870 0.5650 OAS~O O.S2.7Q OA810 0.4700 o.4660 0.4790 2
.o;ssso 0.4820 0.4610 PA~.O o.soso 0'499!1 0,5020 O.SHO 1'illean N-Mean o.e22s 1.ooe2 0,5183 1.0000 o;so3s o.971 s 0.4675 0.9026 0.5138 0.991'3.
0.4623 0.8919 0.5008 0.9662 0.4863 0.9383
.3.'
4 ll52SO *. 0;423Q 0.5480 0.4(80 0.516o O.S4!10 0..4300 0'4490 p,5:m>
o.52ao 0.4~10 0.44!}p ci *. 5~40* Q;5Q1o.
tt495,0 bAS,oo Transform! Untransfotm~d.
Mean 0;5?ZS 0;5183 W?l:i3S 0,4578 0,51$8 OA¢23 0.5008 o.48ss Mlit
.Max.
Oc4~0. 0;5870 0.47~
0.56$0 OA6.10 0.54$0 o.;4300
.as270 oAa1o q;s37p 0.431.0 Q.4990 oAseo o.5340 0,460\\}
0~5110 13.5.89 8,633 7.496
8:981 4.819 s.32~
5,547 4.491 4
4,.
4 4
4 4 4
4
1~Tuiled
.. t..Stat CrlUcal
MSD.
Auxiliary Te$ts
. Sfati$tic Critical.*
Shapiro-Wilk's Testrooiciares normal dI~tribi..ttion. (p* >. 0,01)
Q.9~37 oz7~
Skew
!<.Utt F-Test indicates equal variances {p = 0.27:)
4.19692:
47:4683
.,.Th~e....;c~o:.:.nt;.:.;ro;:.;.l.:.,m~e::c:::a;.:.;nc;.s~a;;.:re"". n-.;ot;;;..
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2"";""4""'469;:,;*.*.*;;;..;1'-* --==,,,..___,,,...,,...-..,,.....,....-=-_,..,
Hypothesis TtiSt {1-tail;0.05)
MSDif.
MSO.I?
.. MSS M$5 F..'pi'.i)b df Homoscedastic tTest h1dfcates no sigr'!ifi~r\\~ diffef;?n~
o;04837 Oi09333 0.0"0205 1;LQ.O;J24' *0.21599 1, :S Treatmel'Jts vs Control-UV
/
\\).7 M
Ji;; o,s.
t e GA
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Ill 0:3, c
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0.. 2 0.1 0 :z 0
ii:
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0
O Page 32 of90
=t g e
.. 0 0
s...T~---:::::'f":'":.":":-::+** 1~~t; 6.05 lelll?l
.. of slgi'!.ifrcar:u::e 0.o
. Species:, Plmeplurles pfomelas
client: TVAIS¢guoyah :Nuclear Plant, Outfall.JOt,,UV.;treated.
Dail
Cl1f!i1ilst * ~
Concentmtion:
CONTROL ov::trea(ea:
i0.7%.
42.8%
85.6%
ti,Mf%*
Intake
... Hard1Jess
, (m" CaCOJill Condui:tivity
.(µmhOs/cni}
A:llmlinity CaC03/L)
aardnei;s
..Cm. CaCOJ/L}
Tern ern:ture,{"C)
Page5 of7 Date: os~o2.,16 measuremenls onl *.)
2
Temperatures perfonnCd at.the timeoftesUnitiaiio~, rcnC\\"l'l ortenninatfoo by the*aaalyst.iderttifie<l iMhe paily Renewal lnfo.rmalion table loea!ed on Page l.
d._lka
ity1 hardn~and total ll:sidual chl'orlrte performed' by the analyst idei,dfied on the beqch sbeetspecifie for each analysis and 1ran5cribcd to this bench sheet
.... ~~"'. *.
ToJat residual i:llforlile :WllS performed on lion"ttcaled:Ouefall I 0 !. arid in lake sample$;
Page33 ot90 SOP AT20 - Exhibit AT20.3, revision 11-01-14
Concen-tration Species: Pimeol:ales promelas Client: TVA I Se uovan Nuclear Plant Outfall JOI UV-treated Parameter CONTROL....,..-=--..~------,.__----~
UV-treated J0.7%
21.4%
42.8%
85.6%
100%
100%
bi take Conductivity
(µmhos/Cm)
Alkalinity (mg CaCO;/t.}
H,u*dncss (mo CaCO;IL}
"TR chrorlne (mg/L)
Tem erati.11'.'e *ec)
H {S;U.)
DO(m0 Ll Conductivity
(µml1osfcm)
Alkalinif;y (m°CaCOs/L}
Hardnt:$S 1mo caco;1L>
TR chlorine {mg/L)
Page6*of7 *
. Date:
08-02~16
, 0**
eratures perfomred at the time oftcstiniiiation. renewal or ienrih,1ation by lhe anruysr idenlifi.ed iii U,ie'1)~_ily Renemu.lllformatien tab!~ l6ca!i;d on Page.I.
Art~ialin y' hardness and. total residual chlorine performed by.lhe analyst identified on tiie bench shectsp~ific t0r eacll an:alygls mi4 Jranst.i:i~:IQ th.f~ b~
sheet
"'"13~****'
. Total l'C$idual chlorine* WI!!! pcrt\\lrme~ on non4reated'.Outfalll0! and Jiltairesamj>fes.
~
SOP AT2. Q.,.. Exhibit AT.i0,3, revisiQn ll-0 H 4
i~ir:~,
¥......, **
.. v-*><*
)*t*
Iii..... ~~
Page 7 of7 Species: *P.imephal~ prorneJas Client: TVA /Sequoyah Nu:elear Plant, Outfall lOl, uv~treated Date: 08-02-16
Concenttation Con trot Non-treated Concen,,. *.* Param~ter tratfon:
Control*
DO:( !i/L)" *.
Non-.treatci:I CoruluC.iMty
(µrn11os/cm)
"Mkaliniti*
Jnig cacO~t.)
_AJialy5f D~y
. (Anal st identified.fur each day; erfOnned. H. DJ). *and. eonductM measurements only.)
]
2 I Initial Initial Final Day (Anaiysndcntlfi!id f¢t eacil day.
ifonni:d R.IlO. ilnd*coildilcuvit
measuremcnlS only.)
"Temperaiures~perfuml¢dll_tctlle timeof't~~iriitiati()~. renewal*i>r ierminatfoncbyUie analyst: identified in the Daily Renewa1 I11!0m1aiion table located on Page l.
Aikaliility; bm"driesS:tli!d !Qlal re:sfdual clildri~ perfomied ~ytbe ana!ysi ideritm'ed on the bemib sI1eetspecific roreach anal}'Sls and transcribed 10 this liench sheet by:
Page 35 of90 SOPATfO-Exhibit AT20.3, revision 11-01-14
TVA I Sequ()yah Nuclear Plant, Outfall 101, UV-treated August 02-09, 2016 Plmepllllfes promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method IOOO.O L'.J
.).11nvltonfllel\\lill T~llng SoluUons, Inc.
Daily Chemical Analyses 1'111jcct number: _____
1_163_7 Conccnt i'nllon Pa r11n1ctu l)nv Q Duvl Dnv.2
... DuvJ
.Dnv.4..
. Davs D11v6 Initial Final.
.lnllllil Finill
lnllhtl Flil11I h1ltinl
'Finltl Initial Ffi1ill lnltiitl Fl1111l
. lnltllll Finni lnH.tSU\\
8.09 7.82 7.88 8.06 7.80
'. 7.76 7.99 1,78'
'7;73 7.116 DO rn111/Ll 7.8 7.8 7.S U
7.8 7JI 7.8 6A 7.8 7.S
.7.7 7JI 7c8 7.9
Con1r41, Condnctlvlh**<umhoslcli1)
)Oii ;,o;'
.318: ::*:,, *
318'..
320ii.*'*'
Non*lrtllltd.
AlknUnlty(m!VLCoCO>l 59./'*,;;j,;:;*::; ;>*.:,:.,,::*
,~
S9 :.. *.
H11i'dJic!S(mglt.CaCOl) 811 '..'.""*..
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9(i*...... *,'::.::::*:;T"''"*
90
'\\ ;' "'
Corit'lii~
ConductMfytumllos/lllti\\
.*301 : ::,;;: *<""'
ns ;

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312 "
'322 "'.'!!>'"i."*' *
!!OS".
311 : ::*:*>'0/*.: *
.. 317 '" *,,,,..,..
uv~trenfl!tl.
Alkuliii11y,(11iglLCnCOJ)
.:59 :;,:~*;::.;,:;G;;;:;,........ :c*,::~:'i* ;2i;;j:,*c:.1.
59,;. ::~;.. :Ao,).. :.......,,
'***'**'e}.*
.ss
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100%
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.oo
... ~,..,., *,:'***.. *":'" '*" :**".. "
sa :>:>:**<....,***: '*:*.*:-..,,....,.* **.,.*.. *
!lU.** :::. <':":.'. : " * :,. ;;;. >;:'.":'.':':.*. * ;.';':.:**~.* ;o:*1 >
.,:./
. 'fcmncrnture i"Ci 24;l!
24.5 24.7.
14.5 24.7 24.6 '
.. 24.7 2~.4 1111 tSW 8.04 '
7:84 '
. 1.91 1~16 7.88.
1.12 1;!14 7.49 8.02 7.85 00.Jlll"IL\\
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. Coniluctivltv rumhos/cmi
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. olUSUl 8;04
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7.!)2 1.11 1.88
'7;74 7;94
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Page 37 of 90 SOP A Tl t~*E:<hibit ATI L2, revision l l-01... l 4
.J
/
Speci~:. Ceriodaolmia duhiit Client: TVA / SeC:nioy~ Nudear Plant,.Outran 101 CONTROL-1 8..ilrvival <1:~if JJ,e.pruef11<:tio11 :P/Jfi!
. Repliea~ *~11.m,ber Day 5
6 10 Young prOduecd Adiilt morratitY * *
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f 6
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SB*='sjil!):'lit~ CiiillB!~broad split b!:C~!Wiiiliyi);.CQ "i2!i *:iimtfu1.,,,;d~ Carn.:d owuw& oili!!t~ittntfet)~ .Co~lce11tiittli!n: %.1\\41)rtali~: 01~ i\\'iean dtTSpring/Feµial~ * '.:~. l CONQ 100% 811ritlvtil mzdll.eproduction i>a_ta; Day 1 Young pi:o,duccd. fl.l.d~lt iiwrtnlity, .. \\....:.. L 'L*. L L.. *\\;* '*,, t\\....... c*.**L. 2 Y.riung produce1f -... p.., *. Cl' **.*. *,/S:1"\\Ci 0... **; *.** 0. 0:..,.... :C\\ . O* A~ult mortlillly ** ; 3 Yo.1mg 'prodli~~d* *Adult morfality4.. Young produced Aduit mor:tali!Y 5 You,qg: prol.lilcc:d Adult mortality 6 i'oung.pnuluccd ',.o :*** o **o.. a .. ("). f!\\*: o.. 'f) _ n ti* Adult mortnlJty 7 Young produced 'Totalyoungpr~~uc:cd * . Final Adult* J\\>lortality NDk: Mot1 il!onofijy :(lo* live. tf ~ dead}.*SB.f' *Pi! !!'!"id Wltglc>briioih~t.l>Otw~1wp d;im CO="""* iffi:r'i'O,..,.;"" c¥1\\'c40¥0r\\\\11lniilllfl lfllrill1tir.DW"1); CiJ!li;enir..a(ill1t:.. 01.* '2i*:">I....... ~~-, *. Page40of90 1 I i .. I . I _] J _J ..J ,~i,s:.. &rtgdni!tnia ililoia.,.. .cfien"(i TV ATSeq\\tQwh Nuclellr. i>laitt. outrarJ lot Da~e: 08-02-16 ¢QNTRQL;:.2Sili'Vivtil a/ill Reprotl1ietitiizD<<tii RepUcate mnnherAdultmor;_tali~y, 'L :L... L.... "\\.......L..L.l.-.. L... L L . 'You~pl'pdj¢~.' ' *O*.. '()** ' o. ' (").. f:'l...... :.... D*... : ~-a* **('\\. ' L}0. -. ~ul.tmor~litY,.. *. *\\_-i \\;,,.;L. ' \\...,;..,\\........* L* : \\........ \\......4 Y~11111g prod!tceq.-:as~__., : _ ?;.. t _ ~-.. ' ""-' :S : CS -~ ~A~nlt.mort?Iity.. : *~ ~.. \\...... \\;.,..\\,. \\.:... * *\\..._ "L. L 'Y~ngpi'OcJJJcea *.6* * (j* *Q . O .0. 6. b o O 0 Adulr~oitalil)* ... L,__.: \\..... "L. 'L:,_; : : ~- \\...... , ~. \\,__ \\.__7* -Youngprod~~tio 1t )"'\\.. i~ -,~-- ** lq,. l~; : X1.J~. * \\'1 \\'5 '2.~ ; i -~:?;). -~\\"'1.C\\. ' 0 \\. "14 ~.,_, --~\\ :.. \\. ~ 0: Ji'.iiiat.AwltNiortali&.. 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L r:-i.. h ~'{)..ts 1'.._() Adultmor~liti *_:c :. \\:,.... '\\...._.:\\.....;. L.. > 'L. ..L..\\........\\...,.. . Mire Ai!lili~!ii:Y: °""'fl\\le.D"'"""~'" ss.. ;splilliniodJ~hi'aod~ bc1we<n im>cla,y$i. Co*tanv Ovi:r-.<orr,.,,;M,,.nicd4'-.r"illillllult duriri~ IQmfcrl. Ct>11eentrnJio11: .o .\\ A L L i1- \\,._ . % Mortality~07.Mean Offspring/Female: %Redueinon from Contro1~2: -1~.. \\'1. Page41 of90 SOPATl'l --ExhibitATlL2, revision ll-01-i4 Control~!Duy J2 3 I* O* il0.:l 0 ,() * : :o. J' o* 0 0 4 5 4," .en *;;. .10ro:13 6o**** *0 *O 716 l6 14, 1'111111 31 31*31 10~'7%ilny .I . L . J 1 O* .. *Q*. (l..~fl* I) *:o J "(}" JV *o + .. *5:.: 4* s: .. 'S JO" H'* ii ti" 0 \\l. '1 A6 u** lfi1*~141'll" 32." 32Z:i.4%Diiy J_l_ 3,.1 CO'*'o* *u-.:ro;-O*0 .:'fo.0'.' 0 4 .)' :.4S. J3'. JO.... I:? ...6.... '.'(J. '..dk O'. .7 '.'14* *.. ts* IST0111r ar.. *n 31.'li11y r *z J. J 0 !f .0 2.. o:* i,IJ.' ;'03...'()*.. *o,. **IJ 5 .A. s 5 ll* *'Iii' '12"(!'o;'(}:0.'18. **1x. ':18 -Tlillllr4;,35* 35Ronllout1h1umh~r **4 Ii.7 il 0tto.o (). "():' l) () 0 0 0 d ~i. . s 4 12 ll* H* *11v (l O* '(} Q .*18 I:>. 14 17' TV A I Sequoyah Nuclear Plant, Outfall I 0 I August 02*09, 2016 Verification of Cerio1/apl111ifl Rei>roductlon Totals 856%a9.IU 1'olol Uny I 23.o.*. 0 0 0 "I 0 0o.0 0 ll 0 2 o*** ll "O.o. 0a..a 30 0 '() 3I if 434 ls.. *s, 10* 12 l{j:' -Ill .!I 14 12, 0 ().. o. {! 6: o.' o. 0 IS u 15 153* .. 1s* IS II!' 3~ *32,30 JJ,, '23* :l~29.306'.'flilut:.*3%.. Jl' 3~ Jt.inlle.iui ntllllll*"'I s6 1 0 0 0 0 "() 0 0 0 0 0 () () lls s'S"'. i312. *12-" II' 00.o' o*19.1119..t;t.' 3G :;u36' *30:.R '1li<*tcni1mtmr* 't"olol 'P~l' ....... :U~nlicnl<>11mi1bor':... A ~. 6. 1 , f!.~..10)*l... 3-..!:.,5 6 .7o. :.. o,*. *ilO O*O*if::(I.."IO,i 0.:o 11'*; O* O* 0.oO (l{I* (i'.: (l () 0 2o'.... o... *o 00 "O .0.o.. 0*.o.-0 ();o.,il <o:3 o:;: *IJ' *u l.l*. *o I.I:l). .:4 4 .4..'S. :.. 3. s:..."\\'...:43 ... *4..ii :s5:...o**.. 4 !LFi..1:?*.... t~~It: 10* :ll* U* HSs:. iJ .l~' 1"" 10 ;n u* 'Ill* ff (j* o... CL JJ. Al 'ILO:.6'o... : o:* :a*. ::Jl.o..o, 11."*IS *iM 11* t:ll*16 T7*: VI".. *::1154.. *7*is** IS'.* 19" *u,.,. l'1 "19 . 3S d!IJ. *.33'. JS** *.w:*, 33'. *3't 31:? .Told I 11 JS J6. ':31 .'JG' '3.'i J!I CtiritroJ.:2. ltcrilleiaii! nuinboi* 5 6 .7 ..89.IO 'ro1111 Rent1rn10 numtt<rrQtiy.I.?.J.4 s.6 1oo.O*'. 0 0 0n*.U. I. .. IL. ~o 011 0o" o.0 0 0 .0 ... o.. .. u. .. 'ff', 0 ,z, O.'... o.... ll 0 ... o.* 1)* :o "Q;.0.() .. 0... ]), (I () () <lo* "ii)': *u.o. 0 .o. n* ,4, *.s . '4 A. 4.,S..s:: 44... 4.c_;***1* 5 1** .it* .*1z. **12: 11.: lit* 1:!' ; 'J4'.. -ll 'U9:"... s 'I()'.13'. :.ir. ::u. :*n....to >Hlo ~0 0 0' :o.. D '*.O***. .. g '(j.. O< *"O' *a Q*.. o* **-o,, u IS.. IS'. J9 16* IS IS".JS Hill. '/*rn*11*. *IS l\\J M '1'4" :"173'4.".\\1%l(f..30'.,31,,34 ':;J(.,373.. .. Tot~I* ;. :29\\ !*33 ;:'31',.. z9-, i"1L 111'. Ji.tto1!llforii iiwnblir' Ti)(ul 4:.,. di l'f*'7 ll* 'Ill'Udy..:Jtif illtntc nu1nJJer* I 23 4.. 5* IL 1 ,<J 0" 0 . lL.. IJ... it 0: (fI 0.Cl* .u: 0. -0* () :.-.o. .0 0 : 0 "U O:' 0 ':O' fl'... ... 2 (J' *o.*:.. IIo.0 !I. -0 'O *o*: *o *:o:' o** **o. llQ.3 00*11.O'.** u !), II~$'.'.'...6.. 4Sj..S, 48.. :'S"*4 .. :.. iL 5...!).....4'. .. *n *Jo 'JJ' :12 ltt: 'fl' CJ f.. 120 ... D . 33 1 l.3'4' ' tl .~!'...dt 0 0 .*14*, lti.: 15** >>lll.. 14'. HS5 'J'f.* .J2 ,*3)* ;,.11:..JO* 333 s Ill'.10" *.14 tt:t ' XL... 14:.n** ..(}; u '.o* .il .{)O 07 .'20" '.Ill. ' ll **rs: l(I. I'S 11* llfnl.. '1$.' >J3* ;)!I:,.;;n... J1. '38' 31;. 8 !) . II) TolUIo 00 (), 0 0 0* il 0 () (l 0 5 ,. Ii" s* 48 1210.Jl:'. '119 .*IJ..If IJ:.* o, 0: 14 16 . W4 J4' *.Jo. 33. 331 To111t 89 ilO'.(Ji> "°' 0O 0i)o.0 O*.... 0 'j)/' .'Oi l\\. '>(i5.S:I. . JZ.: 1.2.. *.'.13* 1!?4o-0. '.Oi 019" 1.:1111:177. 3133 J6 35\\1,ll .. !1. .101 ~ro*lil,, O*ll..* o,.o o* **o (1 'll U* 11 .:o .s. '.L.,I,39 '*.:.12.: :.. fl>.'*.. 11'. 114,0 O* "(}* I). H* 17 IS' 152'113J. Jlh JO$*fl II 'JQ.* 'l'tiiul 0 0. 0, .0....a.,*.. o.... ()..0.'0.l.0 II:' 0 '*l'i"*i.Ai.. A..so'.111! '.12 "I:\\;' i20 '()II-j)'~o .u ts rs* i'75 "J6...3.l *.34.345* Cont~i1trntii11i (%} Control*i 16.7-% 2}.4°/ti 42.8% 85.6% 100% C1i1tirol*-.2 Hl0% l11t11l1e Outrnll 1!ll: DmmeWs i\\*tSD v11tuc:PMSl>:i111i1l1et l>il11neli'!i ~so vnlue: PMSD:. 1 3'i 31 31 34 32 37 29 35 l J. JI 3j,32 32*32 ~2 JS 3~ .32. 34 35 ~6 33 .31 33 ~s 6.6 J.40.J 4.G* TVA I Sequoyah Nuclear Plant, OutfaJI 101 Augusto2.. 09, 2016* Chronic' Whole :Effi~ent Toxi~ity Test (EPA~82l-.R~02*0i3, Method l002.0) Cer.lo1lupl111Jo 1'11bi1J Chrooie'Whole Efflt1entToxicity Test* . EPA-82J..R.. oi.. 013, MetbOd 100~.o QutrOtf Con,trol.Verifle3ti<Ju ofData Entry, Calenhttions~, and StatisticalAnalyses4.34 35 34 31 3(; 3l 29 32 MSD= PMSP"" .lfonli1::ltem1mbei' * .5 (j 1 32 30 31' .3033.35p, 36' 30 3334.Jl 34 3&w 36 35 38<3i 28 3:! 37 38 32 Mii1ii11u1i1 Si1!1iili¢unt Piffer~11cu, Pcrccnl Minhm111rSig1illicn111' Difference s '!f 18 29 ~9 Jl 31 34 32 37' 34 30 37 j3 JI 31 36 33 P1*11jel'!l*n11m~r,: _____ ~ 1..,,1.,..ii3_7 Sm'l;!Vlil. A,*erage rcpnuJ1111tlon Co~llrflcnior Pcr~nt ft!dnclioli fr11ni '(%) (01Tspri11g11~malc)a*rl*Jioo (o/.illllioliiil cunll'ills (cy,,):10 29 100 J0.6.. 5.6 Not applk~blc 32 mo 32;2 6.0 .5;2 31 1ilil 32;3. 5,7 -5.6 j() iOO 33.3 6.3 "8;8 .Jl IOO ,JJ;i 6.4 -8.:z 36 100 t!S.4 5.11 -15.7 30 HID 30.;; 4.9.* Notilpplicabl~. ~4 1110 34;5 6.013.l P,MSb*is n n111osur~onest prccisiOlk The PMSD is lite minimum pcrcenl dtfli!rC11co hetwcc1Hlu: co111rol*nnd trcn1111cnt* 1hnl-c1111 be deQhir~ statistic11lly*signilt~nt iii a whole ~1TI110111.1osicity test.. l.owor PMS[> bound detcnµin~'<l.by USP.PA(I0 11' pereentfloj = 13%. Upper PMSD bmmd dclermined by USBPA {90ui percenlilo) = 47%, Lowet.tmd t!ppcr PMSD'bmmds w1ire detenninud from tl1e*IOlh and 90th pcrci:ntili:i, rospeclivcly. orPMSD datn from EPA's WET lnlerlnboratory V11rlilbilily Study (USt:!PAilOOln; USEPA, 200lb), USEP/\\. 2001q, 200tb. Final Report: lntcrlnboratmy Variability Sht' ol'EPA Short-term Chronic and Acute Whole Effl11e111 Toi:icity Test Methods. Volumes I and 2*Appcndix. EpA,821.B-01*004 and EPA*821-IJ--Oh005. US cnvironmuntul l'rolliclio11 Agllocy, Cinch111ali. OH. TVA I Sequoyah Nuclear.Plaut, QutaU HU A-.gust 02-09, 2016Statistieal Analyses.* Cedodapl!nie Survival *andReprol:locUcm *Tast*Reprodocllon Start Dale:. 8!212016 End Dale: ai91201.S Ti!sl'IO: CQFRCR. Sample ID:. TVA/ SON, Outfaft 1011.ab '°.i;ts'Ett\\llr, ~stlj,,g*$.ol. .sai~i:ileiype: oiviR,.Discbl!rse IVlonl!ol:int! Re?or( ~i'oto¢ot f'WcHR"EPA-621-~:'02-013 Tj!S.tj3peci~ CQ..p!lrieid~ph!Jia dtib.la Sample Date: August *2016' comments:* Cone*%. 1' Conlrol-1 *31.ooo*Control-2 29.000 10.7 a1.ooo* 2i.4 31,000 42.8 34.ooo 85.6 .32.000 100 37.00() in lake 35.000 2 3 31;000*31:000 33.000 *. 3'1.000 32.000 " 32;000 32.000:, 32.ooo 35,000, l 35.000*32'000 : 34;000 35.000.. $.000 33.~oo. 3S:OOO .. *4: 34;1JOO 29.000 35:ooD 34.00Q 31;oob 3G.OOO a~.ooo 32.00.05:6' 32.000 30,000* 31.~~tl 28.000 3MQO 33.00032.QOO 36.000 33.000 34.000 34.noo a&;OOO~EtOOO 35.000 3('.~000 3a-Ooo Transfoi'm:'Untr.aru;formed* Cone"% Mean. N*Mean.Moail. Min Max' Contr01*1 '30;0001-0033 *-so,soo.* 2s.ooo 3;;.;000*Contto1:2. 30.500 1*.0000 30J~O() 2!t0:DD~gg 10.l' 32~200 1.0557* 32;20.0 29~000 21,4 32.300 1.0590 3~00 30.0,D(i **3MOO 42 833.300 1.0918: 33;300* 3tt0 37.000 85.6 33.100 1,0652 33.100* 30.000 36.ooo 100 35.400 l.1607...35,400 3,.ooii 3a.ooo lntake 3.4.500 1.1311 ~4.590.32.000 38.000 Auxiliary TastsKolmogorov o T'*!I i(ldicates normal dislribtJl!on* (p*> 0.01}Bartletfs TesUndlcates equal variances '(p = o.99} The. control means aw not st nllican. dllferer;t. -.*o.89O!lnnett'sTest *'100 >100 Trealmentsvs.control-1: C\\1%. 5.597-4~948 G.001 s:ssz. 6.339 6.446 5.BSS 5.99.S TIJ 7 3Ul00. 32.000:as.ooo.30,000. .32.000 3p.OOQ 3BJ:l00az:oou N.1010 10 10 10 10 10 10 a..s '28;000' ~;OW '31,000 '31'00029,000~(iop*n.0,00 3,4':..00032~000 37:ooct 34--9(10 3q;Oot>37.000,. 33:00()' ,36;00Cl. siooo* 1*Tailed' t-'Stat*... Critic::aj -1.81G. ~.,,s~o .3;oes 22.s3S .5.449 2.281 ?;281' 2:-287*<:281~7 'Critk:al 1,035 15.08&3' '10 29.000 30,0003ZODO~toou .3().()Clti aacoo 36.J)OO* adgo*rsoto.nic MSD Milan * ~Mean'.32~17 1.COOI}' 2;014 32.817 MOODzo14 3Z.S17 1.0000 2.0.14 32.81'1 1.0000 2~0.14 3~.817~.ooo<r .2.!)14 32:817 1,00@ SkM.Y"'....* Kurt.. Lin*oa.t 11\\terp.olaticon.(20!) Resa~plos} Pointso ICOS 1c10 1c15 IC20 IC25 IC40. IC50 "'100 >100. >100>1QO>10i:>* >10.0 >iOO. 40 35 30 g25 " = -e 20 Q. ~ 15 tO 5o.~ c ~ Page44 of900,,0.u ss% ct:*.s1tow. ~ "'-* a:i ~ 0 ~ N e ~. 1.c..--------------.0.9 o.a IWD.Eio:s' gM o~~ o.z 0,1 op-e.-.D-C..,_...,,. ___ _ ~t) *;:*.: ""'\\~:..,.,..... * - "":*~~0.2.o.~-.-.........................,.......-........_.,.~...,.j pg,STVA_ I Sequ~yah :Nudear Plant~ Outfall 101, - Intake August. 02.. 09, *2016 Statistical Analyses . Ceriodaphnia survival and Re:pro_duction Test-Reproduction Start:Date< 812120'16 End Date: aie1201'6: Test IQ: Cc!F~CR Sample m: iVA I SQN Outfall 101 ~Intakel:,kibJD:_ ETS*E'nvir. Testing Sol.Safriple Type: D!\\l'fR-Oischarge Monitoring Report S~l'fiple bate; Acigust 201~ Protocol: fWCHR-EPA-821:..R"02-0.13 Test Species: CO-Ceriodaphrria dUbia ..Comments:.Cone-%Corttro(:.t .control'-'2 . 10,721 4 42-.8 8'5..6 10()".(ntake Co.nc-% Corttrol-1 contror-2 10.7 21.4 42;8 .aa6 10ll f~take. 1 '31.000 29:000 ~1;000 31;000-34.,00032.000'37.oOO35;000 2:31.0QO a3:ooo ~2.00i:i'32.-000 35.000 a2:doo a~tooo 33.00ci-a_ 31,QOP1;oeo*32;oop 32;00() 35.00Q- $4.000~;ggg*4.34.00029.00035;000 34;000 31.aoo 36.000 31.0PO 32".000 5s* 32;000 30.000 31;000. 28;!)00 .39,000_* as.ooo a2,uoo as.coo-33,000 34.QOO 34,(lCio 3$.ooo as.ooo 35,0QO 37.ooO. 38',()00 30;s_oo 1.ooa? * - so.600 - 21rooo* - 34:000 5;597 a0;soo: t;oooo so,500_- '~a.coo 33~boo 4.~48 s2.2oo 1.os57* a2.200 z~:ooo 35.ooo aoo1 s2.aoo 1';0$903_2.30o 30.000 3s:ooo 5.s62~3.~o9 1,091a 33.3Q93Q,ooo s1~000 6.339 33..100 1-0852 -33100 30.. 000 36,000 6.440 35.-4bo 1*:1607 as:4oo 31,,000 38~00()5,835 ~.so& 1.1311 34~$00 32J3oo. JfLOOO s.995 .1 3'LOOO. s:rnoo 35.000 ao,ooo 3;2,00Q 30~000 aiiooo 32.ooo N_ 10* 10 10 10 10 10 10 10 8 9 10 28~000* 29.000 2.9.000 31.:000 31.000 30.0C)O .29.ooo 33~000 32;000 31,000 34.000 31.!lOO 32;0(}0 a7-.ooo 30.000 34.000 3o:ooo 33.000 3_7.000 33,000 36.ooo J6,ol)O 33.000 34,000 1;;Talled -t"8tat :Critfoal - MSO -4:94Q ~ 1 ;734 - 1'404 Auxiliary Tests; -:StatiStic _.***------_ Critieal _ Shaplro*Wilk'S~t. !ndieate$hQtmal.distrib-UVqn (P > 0;01) F:.Test indfo?~ ¢qu¢J*)l~riant:es {p = ();36)' Tne. control tnE!ao~ are not sisnlficanflY.aii'fereot'cp_-= 0:89). 0~9388 . 0;868 1:87805 6~54109 0.13853 -<.10092 MSDu MSOp M$B H?moscedast1¢'.t Test_'iridj~ M-$ignlficant differences 1.49401' 0:.04603 80 . treatmentS vs ContrbH2 .2'.25_- u -_~20.-* Q; -~ 15. -- 10". 5 Dose-Response Plot Q~~-,----N~.----~~,__----~~.--,.....,..~=~.~--~~.----~0.-'----f~ -~ g* ~* t: e s ,g 5 J; o* o-Page45 of90 1-tail, o:os level of sigriifii::ance MSE 3'27778 .Skew 0.22443 F-Prob 1, 1E~04 Kurt -0.7205 df 1, 18 I I 0 I I I I l I r ~' j~)f~~: Specfes: Ci!riodap/inia,dubi~ Client: TV A I Sequoyah.Nu~lear Plant. Outfall 101. Daill Chemis Concentration CONTROL 10.7% 2L4.% 42.8% 85.6% IOO,% 100%Alkalinityi Intake(CaCQYL)',-'rHardness"':L: (mo CaCO;/L) ,,~ "'TR chl!)rine (mg/L).Tem erature C°C}.Page~o..f7 Qate:.,os4u.:16 "'Temperatures p¢qtmed atthe time of test *i!litiatioo. reiiew~ ot temtinati()nby the anatyst-idetitifiediR~e Daily Renewat !nf(lnnation table!~ on Page 1. pJbliitity, hlirdnerui.arui:tqtal residual-~hlorine performed by,~analyst:identili'ett op;t!JeJieqph** sheet specific foreadunalysls 1111d '~bed!O-thili bench sheet by: :'/A age46 of90 \\ 111* iP 5 ::::....r_,.,,.._,.._; Page7 of7 Date: 08-02-16 Coneen.. 'Parameter tration CONTROL llA% 42.8% 85.6% 100% 100% Intakeffar$Incis.<m. CaCO.;/LJTR cblorine tmgtL:JTem ernture {"C)TumjJeratures pcrfonned nt tlietiine oftest initiation, renewal or termination by the analy.;tidcntified.in !he Daily Renewal ln~tmati0.J1 tllble.** 'ocatedon i;age.L ~a~iriity.* ha~esis and: total tesid:'J\\lori)1~ perfof!l'led by tlie*analyst identified on the bench.slJeet sPectfic :for each analysJS and trans~nbed tlHliis bench sheet by:. age47 of90 SOPATI l -Exhibit ATI l 2, revision I 1-0H4 Concentration Parnmctcr oU<Sm CoiidlictMtv tumhoi/cm) Tem111:ratttl'll'l"C\\ .nu1sm. DO trnulf.\\. 16.7%Cond11rtMlv litml1os/cml nll CSlll DOfn111/Ll. Coitducilvltv tumhosfcm\\ 11u.csm DO line.IL\\ , Comluctlvltv l11mhosfcim\\ DOtinl!JLl*ss:cw..CtiniludMlv,lumbo!lcml . 11UtSm Cotiductlvliv tumhoslcm\\Alkilllrilty htil!lb CnCO>l Total ltcisidual.. Cllfol"llie lm!!IL) nUldJI Alkalinity hng/L CtlC01)-'roliil IteslCli1111* Clilorl.1i~ tml!ltl TVA I Sequoyah Nuclear Plant, Outfall 101, Non-treated August 02-09, 2016 D11vo Ceriodap/mia tlltbia Chronic Whole Effluent Toxicity Test EPA-82l-l{-Q2-0t3, Method 1002.0 Pally ChemiCnl Analyses Dn'.l'J. Dn~*a 011rl 1*roj11~t.11umbcr:*_* _______ 1_16_3_7 D11y4 t>nv*6,(l!ltlUI.*Fi1111l 111m111 Flmtllnllinl FinallnltlBI lnltll\\1.Finni ll1lllnl Flmll fulllal Fin11I . 8.09 7.92 7.88 .7;86 1;80 7:89 .7.83. 8i03 . 7.8. 7.8 .7.8. 7.8 ?JI 7.11 7:8 8.0 308 *.*... * ;...-. ;.Jlll :'*,. '.....-as *.-..,.,.*. _.,. ** *.. **.. **~~ i14:1 2s.o24.1!4~7..... *s.06. 7.91 7.9S . 7,SS . 7i:l4 7.89 ,8;02.. ..... 7)1 7.81.9 7.9' 7.8.Al.O20s '*****-~*:*,,._,.-*,:24:8.. .25;0 7.94 7,95 7.907;.89 8.011.9 :*7:8 7;9 7.9 297 "'* .s;o3 . 7.93 7.9S. 7.86 . 7.90 7.89 7.96 8.03.. J.97;8 7;9 7.9.';7.9 8.0 263..................., 24.8 .8,QI *. 7.97 7.91. 7.89 7,96 . 8.04 -228 ;'.' *; *...:.:f'::.......*. 3,01 .. 7;97 . 7.98.. 7.901.92 wo.s;oo*:s.os1:8 7.9 8.0.7,9:8;0 7J) *8.0 'SJ <O~lO '.: *~.->.. ' 2$.0 24.7 !?*k9 24.8 24JI lS,2. 2'-0. " 25.0 ' 7,96 7.96 .8.00 7,92. 1.93 us. 7.99 .8-08 8;0 7.9.'8,0....... 7;9 8.0... ' 7.9.' -8./J '* ' 8.2 7.99 i.92 7.73 1.97 7.94 7.99 7.8 8.0 7.8 7.8 318..*. 317.......*~*. "59,...,.. ~-.: -~- ';i..,~*-**~.... ~*~90 *:*.::.:**'."'. :..,; --**-** -.... y*~*****, *..*.. 24.7 . 25;0 ..8;12 MO 7,91 7,97 8.10.. "91 7.9. 8;0 s:o.* 1.9 -1.9 .29S 306 . **309 *.********.... 24.88.10 7.90 1.92..8.0 ,. 7,9. 7.9 '.;283 :... 296 301 8.09. Ml 7.94 8.03 7.93 s;o 8.0 8:0 1;!1 1.9 s:o ito 7.9 s.os.o s.o 8;1 I "7;93 .7.97~;02.8.1l6 ?;96 14~9. 2$;1) %4;!) Jl.IO 7M 7.98 8;2 8.ltkl 199,,. : *:~.;': 305 ~d 8.03 7.9... , :;=-~;: :.:*::*:-:*,:** 24.~ 8.06 8.l ,211 '"'.,, 24.9 7.98 8.0 ., I 'l~l \\ ' (i.i ~Fu~ $Qn101~080216Cl1e. i11XJ*Entered by: J, Sum Reviewed by:+ .Arialj!St l~.f}fp Dare*am~lyzedif :-.oz.::/~ CalihtaiUm';... Total Residual Chlodne (OJnON-1977) Matrix: Wat¢r, RL"'0,10 mglL Page 2( Page_}__ of_/_ Meter: Accumet Model AR25pfUion Meter Iodide reagent,1---'/_N_JZ..,,..]=_.__S_"l> __ -t Acid reagent:..... _J_'.'fi}_Q_f-_4_')_~uo.mglt NQtq.* ;f"ot:s~ples 'Wlifr a ~!dual chlorine bf ? LQ mg!(,, the samples miistbe;diluted lt> be withfotbe ealibmtion range.*Laburatorv controista11ilanl: number Sample number 'True value (TV) .,(mg/L). 0.50 Sampie: cha'ractedsncs sam;ple Sample IDnumber, Measiir value' (J,\\'IY}(mg/L).Residual chlorine *(m IL) s,to.. o~?I %RS='MY /TVx 100 (acceptable range=.9D to 111)%} %RPD = f(S -D) /[(S+D)/2 x 100 ,.. *acceptable range =::: 10%) >>£.o~oz~:L-: ~,,....._-----~ Sample cliara~teristies Residual chlorine (m.L) LO-oo/i/r Referc~ce standard Tru~ vnlue (TV) Measured vali.le (MV) " % RS::;:- MV /TV x 100 n.umbet(mg/l:,)(mglL) (aeeeptable rn.nge = 90 t1d H)%) Reviewed by / (ft Dote* reviewed._t-_-__ :_=-Cfl:-'*~Q'l.=-'*-1=~====='. Page49 of90 SOP C8'-Exhlbit CS. I, revision OI-01-13 I I I I I I I I I I I I I I I I I ~~* lf Pag~ _'l_* ~f-~_ T:C),tal 'J.tcsidJ{~tChl~rin~(O~ON.,i97~ 'Matti!<: Wa$7, R:L =*0,10 mglt Analyst ING *.Date analyzedo6-o~-I~,Meter. A~u)]l(lt M9del A.R.25 pHflon 'Meter 1*::::: 11:m Calibratitm: Referenc:C.sta~thlrd **tr ue.v:JJµ_e(;f\\I}number{mg(LJ. Sample number. recisioi1:.. Sample IQ .* *saniple chara'~terntic~ Soni le measurements: Sample S'ampfoID number 'Re5iduaH:hlotitui m ft... s.jjp.,£170' * % ffSi=.M\\t;ti'VxilM ta~~pt:ibtc r:a~e-"'*9ffio;.t~O%) D C'q~j;~Jt) * *-* -----""_._..,..mD Reference standard True. val~e (l'V) Me.asurid value* :(1\\-'lYl'°%R'S'= i\\:lY'tn.r,i 1~number (IJ1g/L) * (ing/L)... *fa~epti$Je.tnug,c=:pa:fo*lill0A>l '* Reviewed'~y r. I(,*.Date reviewed;.. *,j~tr. Page50of90Tqt~l Re~id~~~ CJ;J1ur~ f0Rt(JN.:t977l Matrix: W~ter. Rt = l:U o tng/L Page. 28' Pag~_{ _of _L_Meter. Accumet ModeLAlU~ pH/Ion Meterllile=:li--a-1E. J 1~~-.~...,..;~-~.,.,...**47J~.~----.Note; Forsamp~'Witha ~~dual cbl~e of > L(hi~4 ihe samp!$.11iust bci di!~ tt> be within tile calibratio1J i'angc,' -~. S:u:nple* .**number l);u~:'.Value (tv) * (izjglL) 'Meas11red'valu.e (l\\IV)'. (ffi,g(L). Sample cha:racteristks N'i11e: AllSilm,~les.'\\".!!rrNliml)'zei:J'in*ex~ofEPAremml!itCled'hcildin1J1i1. . La/JoiiitO. *.. *c0ifli4J:startdat'lf:..~rmn~standard * *nutilblir:Page51 of90 ~~~,vaJUe (TV)*. tn'ij:/i:;),. ' l\\f~iir~' ¥:du¢* '(MVl (lngtC.l 3 RS= MV I TV x*lOO {!ltCcptab!e range= 90 to HO~)Reviewed b_}r 11-. _ __,M------1 Datereviewed..... -~*~IP~.._,'l... fk..._._,Jli:.=-* _ SOP C8-ExhibitC8.l 1 revision 01-01-13 I I I I I ._J J J J Pa~ ~l Piige; I *Of v Analyst' 'I v-I.*.Dateanalyzed......-'.00---..-w-...... ,~-. -'""I Titram i1omwlitv.and:mu/U11/iet-tfetermb1atin11:. pH of l'ltmnality *1*Norina~t)>*(j\\t) of~iS:Q4 Deionized T:itrant -.:h~Q~n. *' 'Eiid Total.=($'-miNaiC03x o,~s)i:E'pH F,:i~r*orM~l(ipl~er=(Nli. $JOOQ)IIO() l1lisan1ple* ::N;c,.'.so.o wa~. reference. s(a~()al'.d.mt : ml ml c= 0~251.t =4.SS.U. number number <El*. *(ncJ:cptable~nge.;..,l)J)ts~*~2ii) Referen~ standard number Sample numberTrue vl.\\fu~(TV) {mgCacOJi'M ..J Duplicate (Bl Matrlt soike reco11e,.Y:Safllple yqJttme cin1.H)O..Atki\\liniw**@IY). %~.=MV'iTVx 100 Begfu : End Tow.*; M1dtipner*.. ~(~~.C~Ll**(11.ccepta:blc.nmgc .mlml nil '.. * * * "'!10 tQ:tt!);.OAif... -*,\\\\:ll(li6riity...$RPD"=Total. Miil.tipli~ * (iilgOiC.Q#Q. * * {($'~*:D)fl{S.fD).t2J}x.1op ml.. <*'11>l;abie range =.;l; 10%} ~.l. *.** t{)C),. ;S Reference $ndard Spikla v11.1µ~. Sa111ple number .. '(SV) volume "(mi!:CaCO.JL) Jml}, Beg!n*ErW:. Totili'mlnii. i *~***Samole mtas11rf!l11e11/s: Sample number Page52of90S~mple alkaf!nifY (B}. (mi. ~co~L) .M~asured ~pi!¢ \\jat"ue (~VJ* ** i\\'I:V,,;;;A.... :i,r. (me. CllCQVL}. Begin ~~d ' l'crtaJ %':a=:MV /SV,x:lOQ -{ai:~pt.dlt~ range =-1Sto:il5"4')Sample;votum~. fin.I} inf., m1* . ml*.Muiti1>1ier AUcanilltY *(me CnC0.1/L) ru* .., U* ,(po.*..... $:ia1 l1--1 1 -*1 t I j . i l l J l J J J _.J o.:0.:~- .S s=~~~J;:::L .. A~tl **LG,.. f~ .~at)'ted** OO~P'O~ni A.lkalinity. (SM' 2320 }J;..J.997) Mattix: W.atet. Rt= C'9 mgCaCQiLTitrate*saJ11ples to pll = 4~s s~u.Page ~2.. Pa.,,ae. 2 of_V..___ Time initiated,........_uQO..llllts Time completed. TitraniutJtm.atiiy 11it.d:11~ultfpiier det~11i1i1itfim~: Normilify heckI. Total,..fererice. stan~~id: *.. mf.Normality,'(N) o~;~so.r"::i{S ml'Na1CO;J x\\U.1)5)/E' =o~sm**. pH Factor ot Multiplier (N~*50000)/ 100 ml sample --iV.x.500 = 4;ss,u. ri *number_ l Mmber: 11..* 1 -mi*abic ri*=i'o:Q.1so ~o;~)......,.. ~.........-~---,:.....-*.-..:-Ui.b.t1ril,tofY°CQi1tt..Ol sltfi;tlti.td: 1l*ter~::::~nr*1. *. ~rtr~tu~. ', **. ~~: . *.(gig ~COJ/t;)_., (m.l) "**i *Alkaliaity (MV) B~g~~ I En~ J** Ti>~t *j Mtdtiptter** (~CaCC>.1'L) ml . mt, ml ~;NSS l~*U\\;L. ].. 10tl. t 100~~~'3:-fHilF:q.. 8 I lO:<f *1 qs'i!i:i~oit: . $ampte:in * ...,.,,~. *.11... : ,.#.~... ;,,.ove"'** .*l~-iuir..,,,sp~..*..._..,.* *..r** -~1\\!i Sample -volume J. B~n *.1 :End 1 *Total(ml).... ml * *ml. mr ..IPO I *}t'o;o 1111:1o, !Jo.~ .J; J \\~\\# J.21.. l Uo*S:: A.~alinity. MiiH,ipliet I (mg CaC03lL) J 1.s Uu fD 100 %RS=MV /TVx 100 (:u:ccptable range =90to 110%) qe.01, %RPD= ((S -D) /[(S+D)/21} x J 00 (aeccptablc range=::.10%). Refere~~.Sta~°nrd,..Sp~.~ vain~ 'ntlmber * (SV) Sl:tmJ>le* 1/oliun~(l'll1).Jl~n 'j End. I Tot.al j\\IJilltiplier Spike alkalitiity (A){mg CaCOJil..) <rim' cacil31.L>,-ml. ... ml ,. ml* lJ4~Sl~lY 50 \\t:Q l\\\\"" I ~;'&I! ISSJfo .o ~lSbsamtJle*lkalu:iitr(B). **(mg c~~OJ!L> ** * *.. l !)°(). Snmoie m~l1S11rl!l)te11ts: -Sample number-n~t;tt.?A O~.!ll~U"* P.> Sainole ID.~-.,.*.. L*ebJ .,,.;;;,J~:'****. J. .,*Jo: n1~,.<S) J. tu~ l\\rM"~*:"i.~:.J~lfp. lhb: 0()\\ i~ti.t<Ps-~"\\.l. l. !fm:tl\\)T1* I J'J.~,It. bl~*"Li * ; _. rrll 60\\_ * .. t)g:Qb.. ~*!'. J. i'i. :j <ft.. .lb...... :-\\I. rm1N" .,.. *1** I'. ..L . 1\\IM.~.(J~ :'l~t1Mil;tt0£l. l\\o<5l-O\\~G\\ l'-rW6A l.Sample.votµ-me (mi)'..* .oo J-26' .ffij. 100 %'R=*MVISV:x: lOrJ. . (lll:!cepilibf¢: range =7Stol25%) Begiti.*, En.d I. Total -ml . *mL.. ml.* Multiplier ,;t,.tt 1.$l1 I G.; * .3<;:~ 1 1415:01 ~ti\\ o~µ 'I 1-J] i.11i~ 'l..1 I "Li I..:a.a 1r1"\\t.... -.f,. -*,,-.,.... ----.... \\!~'!I-JJ,ti. *J.1s.. ~1* 1 ~g: ~l. .J-""**1: J."**.... 0-**.1;t.t)*,,,... c~WJ,. LJ.J4.. . :"I.W io.ol:tasJ a.s . '25.tP. l~S,I l:q, I' 25 7'-1* "l1l:i~ :1* l l f ..."::>. '"--..(",,. ' '°1' ~ 10;{) 1£ 100.w. .~........ .Alkalinity (ing CaCOJIL) qq ""'**It * ~cu 280"(JJ ro q-z-=ro 313 '-IJ Page 53 of90 RevieWed by: I ~.'. f Date reviewed: I o&t>\\.*1 b ~OP C6 - Exhibit C6.l, revision 01 I 3j J .J J ,Afkalbiity (Sl\\1::2340'".IJ~l991) ,p~~ ltJ ~ Pag&. a or. 'V Matrix: Water:~ lU, ~ l,-0 D18 Cf:!C03'1;;.Ttttat* sam)>~ *~,.:;;~,411i~ f'~ pH= 4S s.:u. Ti1rm1t llormtilitv a11d n111lti1Jlierileier1niliatiort:' . I.:. pH of. Normality Deionized Titrant check'' Begin End: ! Tl)~l 'ml 'ml*,f9tii#t1it;y (JVtof'li'iSQ4=ts mUii,!hCOs ~:OilJ$)~: _(JjS/jliP.¥ JfActot. or :M:!lltiplier= (N'X :.soooo)l 1on*m1 sa~pte.* '""'JVx,50(} water re1e... ~- ~rd..~: 4.5 s~u.. number . namber*'Lnboratorv eo11trol stamlard:.Reference stru1dani' number 1'.ruevaiui (TV) (mg ca~().s/L)iOO sa,rople.*voiume (ml) JOU ' *able J'-iing~.;, fl~Ol~O ~ 0:1!220}:. Begin . End, T9tAI, n11 1111* 1111 i A!kalin)iy,(M\\l.l, ' %'RS~MV/Tv,;<JOO ~uJtlpJi~J" , (mg-'CaCO$Ij) * .(~i:ceptallte 111~* = !J~.10.IJPo~>* * 'Sataple .Alkalil!it;y %RPD=*v9lt11n¢.*. ~.~ ~ : *.*.'.Em:~,d.* *, Total '!Mu~piier (mg<f\\.C.OJ.14. * ((S~D)Jf(S+DJ/Zl}xl '.(ml).tiil(~ci:~iablecan,ge,.,,::1:;ro%): r a.~.S" *93ef..... iA.o*... * o, Duplieate (B)'Mauixsoikerecov.erw Reference standard : Spike val11.e ~mpfe number (S\\I)volume : Begi!'I, End Total J\\lful(ipller (me CaC03/L) lini) iiJI. mf. '., ml Smnple 11ieasure111e11ts: Sampi,e alkillin~ty. (~)* (!U~,CaCQ¥J:,)l\\fe~ured spif<J.1.VafiJ~.~~ MV=*A,,,B{ingCaCO,ilb) *. %iR=::.MYfSVx100. (accept~bilth.R&c, '.=;7ftt>'I.~).*. I,.... 'O\\.~.*:. ;' . U\\..I'~'-- ~ Samole number SamolelD 8aJJ1ple vp1t1me Begin. * :En1:l fmti .J ml'11t1, To~i.ml Mulfinlier Ait<aUnity*lmg CaCi>.J/L> .\\f.l," Page 54 of90'j:l l J J I { ~"' Page ~lf)'-i .Page '-l of lo .Alkalinity (SM.232Q :B~f99:7) M!itrix: Water, RL= I.OmgCaC~/L . _Tbne initiated,.:'>... UPo\\\\I Titrate sam pies to Time completed "'ill' pM=*4:5S.U~ Titrantrzarlitaliiv *llf1d i11t1Jti11ifer ife1em1iltiitfon:. pH of:N'ormality Defonizecl * Titrant'Clteck; '\\ &Le, -* *nee stitndard, 4$S*U~,. nu'nibernumoer Lb It: orli(orl~1ifto *$fan 'ttii :Refet~,n~e;standard.** Truevabie. number "{~V) '(mg.CaCO,Vl,). \\ S~l~\\4. J;90 Matri-c sJJike t¢e,overy: ' R.efenm~'stam:taril 'Spik~ v~llle.. number. {S\\r)(iugCaC03'Ul Nor.m~Jify (N) of<l:liS0.1 pH Facror or Multiplier'IJeginEnd *,'to,t.11=:(S:mlNa:iC03 x Q.OS)IE ml, m* m'itl:.25/E *= (Nx 50000)/ )()0 ml sample Nx500 m'i.., tai:teptaJ:iie range>= ~)lt$o ~ 0.0220) ~::unple Alkalinity (MV) % RS=MV /TV x 100 volume 'Begin '*End Total ~ultip)ier (mg C'lCOJIL) (acceptable range <<mn .ml ml ml =90 to llll%) lOQ.leA 'Z.e.e ct.ii:\\ toLo q~ qq,b/ S.rimpleAlkalinity%RPD= volume Begin t~tl ml . *End Tntat * ' Multiplier {mg CaCO.YL) {(S ~ D) /{(S+D)/2)} x IOI} (acccptnblc range = +/- 10%) ml ml S~mple vriiume Begin End Total M*uitiplier *: (nil) nil mJ .. ml JJ:sZ Spike alkalinicy (A) (mg CaCO.,/L) smnp~~a1kaJin'ify CBJ'(m~ Oitoa!LJ 1\\1eas11red spike vahie*(MV) lWV'=A-8.(.m1t CaCOJfLl % R=MV1SV:x100 (acceptable range =75 toi25%) Sam pre number : Samnle lD Sample volume I . (tri}) )OO I Page55of90 ~egiit m1. End nd' Y*lil-2.1.sS:* tD0.0). Total ml. Multinlier .... I o:o Tl I "'t~.o Alk:tlinity (m!! CaCOJiL) Bo SOP C6 - Exhibit C6. l, revision 01-0 l-13?.'\\'Analyst I :a. I t Date anatyted . 'i\\Q. \\p.,t>age_.-~*l'a$e... 5 of l a Titra11t 11ormalitV a11d 11mltwlie1'<det.enniiu1tioit:, pH of Nonnapty Deionized, Titrant ._....,..~ water reference s~dard 4-5 S.U. number number Laboratory eonti'ol stmufani: Referenc¢ standftl:'tJ numberTruevalue~mple (TV),volume:* (mg Ca(;Q.VL} ; lml) Matrix snike reco~*ery: lf "*.... e.gJn.ml N.orrnqlity'.(M.'Oi'~¢:4 = (S ml N'a2GOs~ .. ~.OS)IE: ., =@.l5tt. pH Fl(tfoi' pr Mll)Uplier.*;;::*ii\\t':X:~OOQO)/lUOmlsample~Nx -soo*,... ble-t;1.?o~t~IJ-M~OI ~-**-~{(;End l Tohil ml' ml Aikaiiitify (MVJ % ~ =<,~/ l'V :X 100 (ll'!g.¢ilt<WLl ** (aeeeptabl~l'!lngi: =~O:toJt((%) References~ndard *Spilfo;value.number{SV)'Sample= volume. iif;!gin. End.. *.(Din Oil..ml total,.Multlp)ier !ml*~i>i@attqilmity (Al * (nj~, t':lg()JJL)*{m~.CaCbJIL).Sam le meos11remei1ts: Page 56 of90. !>!iinp~:a1kaiintty;(~)(mg~ Oi003'L),. -f'Q '*= Measure~f$1Jike; valn~ (~ ~=A"'~. (me caoouty.. ADY'.. .. *t6Q:%,R~~VJ*SV.'x10!l (accep~abft.'rijilgc'. "';' 75:19125%).. Reviewed by* r . f .!)ate.re~~ew~; t.bi* 6~~\\'° .1 y&c*SOl?;Cp.'- ~xil,bit C~;;Ji*:Ie"'.i$i~n.Q.hOl ~n l l l1 l'l I .J _J J l J J J ,(*~;.. ).~~ri~:;~ Alkalinity ($1\\1] 23ZO B.;1997) 'MatriX: Water~. :llL = l,-O mg CaCO;/L Titrate._s~Rl:ples* to* pH=4.Ss.u~ Page "'3£g Page (.p of Lo ..Time<initiated t :.............. <Je01lt Time completed ~Notjtitditjr ; - *.*. :,* *:Begin ; ~d : Totaf *.... 'U ;..1-, bclbn.tea*** Titntrn; pH Factor or Multiplier Ntn'malify (IV) ufH1SO-t',y#.ter *. r~feten~ -"::<4;;5,S;UL :.<1ttimbet *. .$fandiirf$.r ID* ... :::iL.ml number* * {Ef :_ (5 nitNii CQ.l x itQS)fE=IJ~iS/E.. &ter..ang~".""o.01so:-~o) * = (Nx.50000)/.100 ml sam1>le =Nx500 Refer~ce sta11ifard l'tu1' value Sample.Alkalinity (MV}volume Begin Ji:nd *Ti!tal ** Multiplier;:(ing CaCOvL) %RS=MV /TVx 100 '(acceptable range =90to 110%) numb.er ,, (mg.~~~~rtnl},ml ml ' mr References~ndatd n~ber Page57of90 Silmple AlkalinityBegin-*.' £n:d*Total Multiplier (mgc.1co,/L)%RPD= -Si,tb!plcffD. -volume<mn. ' rnl' ml ml '{(S - D) /[(S+D)/2]} x I 00 (acceptable range= ::1: 10%)Spike value~ample ' (SV) * . voiumeimreOiCO.,/tt'(mil $ample a1k1!Jiiiity:(B) '(m~ cBCOJJL}. D~ln - E~d TctnlMt!:ltiplier '.'ml. *. *ml * *mlM~surecl spike vnl~!i!-(l\\'IV).MV=A-Bf. CaCOalL.~ : Spike alkalinity (A) . (mg CnCO.i/L) % R= l\\iIVISV'x IOO (acccj>tablc range = 75 t~ U5%J .OJ. Samprevolu1r1e Begin* End* TDt81 tmnml.. ml mi Mtdtiulier Alkalin'ty lin~ CnCO:i/L}no 3000 ZCXJO ?.1-v"JQ "2..tbO Date reviewed: I t>&*b\\ii"l!o , SOP C6-Exhibit C6.l. revision O 1-01-13 .. 1 l l ~l l l --i I l I J J J J .,. / 0 '!l ~'. r i"., "'~i? 1 i * -1 ... r--; s_____i-,~. _./Analyst I ~ '. I Date analyzed .te~Qe.!l/1,,f Ji:~dness (SM 2346C.-U97)RI,,==* J,O.mg C~OOJL. Page . Gf _j Time~ initiated: *1*.ti..fe I Time complete<t....... :_.ty..:;*__ :z.......~_. __ __,_ Tiirant 11<Jm1alftj1 imd nmlifpiieiileremJii1tlti01i:. TUra.nt.J;onn***lllity cl .. 1eck1* ' ile¢in. 11 En~ *1 T-0ta. *.t refertWte s~ndard ml ml ml number... HI number (E).'Norm::ility {/Y} 9f EQTA ...0:0~21.E{acc:cptab1c-.r.aii.1~ ~0.01~0-~o:oµo) pl:t'factor or Multipiier= QV~ $.t)OQ0)/50 :pd sampie =Nx.1000 1tJ~ t NS4*,"Qafl 6.o '! no t to.o0.02.00~Q.;0 1~/Z.~ Laboratorv -con/ro/ sta11dard:. Reference.standard* number l~'Sa1;.,.oz.Tfu~~1ue* *1: Sitmp11?.(iiV)* . : voilime m caco 1;; (. g..... 9. ~......,,{mil 401 sc;. recisitJn:S~mpJelDB~gjn.ml lO.;.u BegiJ) rill End *1 Total . 'ml*.. ml . Hard~e~s(~ :1* WRS=.MVI'r\\i xlOO M:i.iltipJi~ 1* {mgCaC03'L).... . *<<:l~cptabl~ n1n~1~1. J t<!l. I 20.0 End ;I* Total* I Mliltjplier.1111mt Hatdness(!JigCaO>Jll,).=.90~U0%}fo5.01 %RP.D= {(S.;; D) /l(StJ>)alh 100 ~ "l t '*'.k1 I -i~a zoo** s 110 Dupllcate {B)l.Matrix soi/re recoverv: Reference ~nifard.1 .S~ilc¢ va* .. *.. l~. '. *1 Sa.mp.!~ :number~VJ.',vo~ume* *. (in~CaCOJll)).,, _(~J):hl~~\\'hnJ 4o,: 1 *5b Sample 111easureme111$:.$ampJ,ehardne5s (B) (~gCaQQ#L) QO l"tl )fl-:1,1 a':'o J. . e>. *Leto*. ~e~in 1: Endj Total [ MultipI,iet::. ml:ml ml *~pi~ hard11ess{A) '(iitg, (:!l'(::()jfL)l~J ha.t *I Ll a I tcJ.o.*M~sured SJlike."'1lli.te(MVl MV=A-B {mg QC()j/L). '-lo l?>D %iR=MV1SVx:JOO (accep:tahte range. =75rol:Q~) . 10001~.. Sa.mole number .* $amJ>fe:.iD Sarrip::Jf lum~ f B~f r~t r!~ r Multiolie~. . Hardness ... (~ CaCOJffil TV=:ND '.Blank {slioUld.be=O'mgCa©il:). oe.oq,Jk I.ssw lo6ot.lv fl I mHG:.W*oo~oi. u~ g ~1-\\. if3~o"f.\\v I1 Ul--z~Ji I 1 sst;J O~-a.~.*~* f -m Hb.trJ*1 @* 'Z(o.4 V I f --cf Q'}.t9_.U4 O~-~C'J.Lt1 'Sb o.o I o'!o I.~:.o .20.c;>. .l\\C Je.1 1 *io,t I t.oI Lfo.,;;to~t I Jif.~*1 t.1,;.e. 9t11:a1;q 1'1.i-f I 4~*<;.tlo.~ -jl;c.t l Jl01.Lf.* 4 B*t5**.4* -.~. 6 I. ~et z:.o*.,.;7..J *... ~ 40 ~e ltti,.flll~~ Cl2 )'.IJJ.w l~<:)*I &l~**qo Jltfl8 ~-"1,q~~~*I 4 <llsl&::J.0.92.O*D!*q~~\\ IAet'IM Mn~~ Nore: If >tSml ofti.lrant is oscd,,Sl\\n)pie-~ be-diittteq. Reviewed by~ I I Page 58 OT 90 Date revieWed I. .64-o~~. I so P C7 - ~xbi~it C:7 ~~ *. ~vi'si0fr01-01-n l l l l l1 JJ _J .l J I J J J Je g.~b *~ C.'":~)J. ~nrptl)tal~~'Wlo""b!C; ~r£**.** 1 J)ate.anal~* *. *06.D5.. ~y, .Hardness (SM Z346*C>l997} 1lL = 1.JJrng caCOIL Page __ z..._of_~-- Time initiated,........._ , <8$f " Time cOmpleted. t1'1ra11t iiotmolJJv tuiQ*~1f11ltiP1kr*detemu'iliiiion:.* :.,.* ~.: *.. ""' * *.,.._....... k..J:iegin~ntirilf~ei(,e * stanefatd*m1 *.. tu.imber* *. number Labomto!Y:contr.oistanlliirii: *Rere-re~cestan~~rd True. value num.~r (TV){mg~11COJ!L}*'(*~mple volume {mi}' so.$a.mpk:vofume'rid) Tofii.J. ml. (:E):..Notmalitj.(.tV) ti( EDT A...o.2'!E *'.*'-le ~ngc ~ o.oiso~ o;onol pH 'Factor or Multiplier. (Nx 50000}/ 50 ml sample -.Nx moo Hardness (MV} % RS= MV /TV x 1-00 (aecepta bte.r:ingc Beghi End'Toint ml ml ml Multij>l~et (jngCaCOj/L}= 90 to 1 Hlo/o) .Hardness %RPD= Bi:gfu .. End Total Multiplier (mg*CaCOJIL) {(S - D) /((S+D)/21} x 100 mt .. tnl ml: l~ ' 61)~;..(. 6cl 2.z i io.o s44..J. , Diiplicate (B)1 4. ; S.t1 ll>i0 Referencti starida:i':d $pike vnlue *.$ample 'number .. * (SV) . "volume Begin : £nil .. (me'caco.Jt)(ml}mtnil 2;z..Total inf -1 Sampt~.fuirdness (B) {fu~aco.itJ:,J 'Measured spike value. (MV) ::MV=A::.... B*(ing CaCOJ!Lf 40 0.* Samplev~!Um¢ Begin Eud. Total 'f4 ---..oeost.ip Spike hardness (A) (mg CaCOJiL) %R=MV/SVx 1.00 (~cccptilblc range = 75 to 125%) IQO.lfJ. Samole-numbei : * .Samnte m..(mij.Jril mt' ml Multiplier Hardness (m2 CaCOJfLlB.. l....:'"....T\\f===*Nn*~lj\\;{sn0!.'11<1,be=u mift;aUJ:ii.W *?,Un llti - L 2.o se Ll:z. - &JO . SOPC7-ExhibitC7.I, revision 01*01*13 l 1 l l l I J I _J J J J J 0 ~ -r,*v,,~ I I ~- '"'""*** I \\ L___i -* EmiTDP111tnl>l~$o!11Uons.lne Analyst I IN. Date analyzed. CJ6.,Qs.iy I:Iard~ess (SlVI~34fJ.C-1~7} RL.;;,. )..0 mg; QaC~/L. Page ~O Page:.a: 9f~Titrant 11or111alitV and nr11/ti11/ier,lfetermi1iaiion:Titrant Normality check Begin * End Tot~I' Notma,.fy(Nj; iifEQTA ""0~211\\t .*.(;a Fi,ict9r or.l\\'iultipltet* re!.A_.... __... ~ number number fE). (~~cptiblc ~~ = 0~0.180-lU~tl)* .*** *,:=.(N:x SOPOO)I 3tht~ s;unple =NxlOOO Laboratory:co11trotstoi1dartl: Reference st.'lildtJrd number Sample number True value (TV) (mg QJiCQ;i/L}'. J) Matri."I: spike.recover_v: Reference.standard .Spike value number(SV)(ml! :CllC03'L) .*Sample. voJume (ml)' Begin ml* ilardnc$s:(MV).. %:RS:~tvITv.x 1011&nd Total*1"ftiltipJ1et* (0tg Ca(:i9J!~l * * {ttccci>*alile tnnie ~ ~*:. ~~~ so SnmpJe vofoine.. B¢gin End T$1.. Mu,ltipljer (ml) * . nil ml.inl * ~.*., Li"'..;.J'4$.1:.I* l.. *. ~- ' Samplevolume.(ml) ..50** .B~ilt. End101al ml.ml', ml* Har.dn'55 %RP.n.:. {mg:4,CO,iL} : {($~'D)./!($+D)(l)} x IOD s.* z.z**,.. $pak.& hjtun~ cA.l(~g: <:acoJII.)Sl\\mple'lmrdness{IJ){mg*CaCQ.VL) Measured.spike vafu~(M¥) MV=A-'B % R"=;MV tSV':x 1'111 {1!¢~~le:rasigi;.. =..i7Sto*125%) ~ Sample meta11remm1ls:... Sample number .Samote:tn 1 0.~I .{iJ!aOit:OJILl $itmpfo *. y,oJuine * 'Bigin. :.E~d : ;f,Otn) '.ff~)'.d~ess !miOIC:Ll tmn int ml ml.. Mwtinliet',. 1 z,~\\'J, ' : ~ [~a.~ r~viewed J ****. f>~.a~\\f SOP C7*;,,, ~~ll?it<Cm J, revision P 1*0.l"'Ul l1 ll I -i j I J J I .J J J J 0 ~** IE:: ~. :;;t; j£il~~~~"'"' Anfilysr,-l;C>.--- r J)ate:anaiyzeq,. OOX;Jt,.* lV. . ~. Hardness (SM l340 C-19*9~ RL,.,,. LO mg*CaC03/L Page Y I I. Page of b .. Tiine.foitiated Fl!~t\\?) ---] Time completed l-Sa..1 . Tltmnt ;1pr/11'1lftJ(tl11<l n111itifiliet,f.teernlii1at/Qn: * . Titrant *1: No.m\\iility:tftel:.k':j*.B,~gin1* End *I* Total reference*.*standard. .mr *mt*minibtr.. *. . ilfil.llberml tEl NcirniaJity (NJ of'EDTA...i-0~2/E, (acccptilbJc rnnge"" o.01$~*... O.IJll(I) pH Factor or Multiplier = {N); 50000)/ 50 ml sampleNx 1000 IM~l tr¢5sl3a7 l o.t) __ ltt*<1* J *q.e a.01.otl ta4 WtiriJJif!Y °(:ontPJI $/mtdai'.11:. Rere~~~~~~-ro *1 ** trtu... ~1-ye * .1*... :~:~! (~g G;iCOJIL); .(ml) r"N.ss1x:i02, 4Q I so .. "iJuii1iCiltii$li.iitJJ.{ept~isiim:sample;*nuiil~ s~~pJem. 1'4*1S&c.H~~\\ tr~ '\\..'~lieme(~) MmnxM>ike~covm: '&lmpl~ . volum.¢ ... *-. cm'o ' '51J j Beg~n1* End J* To~I mt.. *. mt.. nil 1q~a Jn.~ t t'l Begin *1* Encl,. Total ml.mt. ml I\\~ I *~~... I *~d J~~.*.1.~s~z' t'l- .:ifamriess (MV) Mattiplier I On~ OiCWL) 'tb~.J.aq .Hardness MD'ltiplier I (mgCaC03'L)1;aq *1s* 3r. Io.!.. ;. 3~.%'RS= MV /TV.x 100 (aci:eptable. range '=90 to BO%) q'l.-5/. %RPD= {(S-D}/[(s+D)/21} x 100 5~Co7. R~ferenC£i,*stattd~* *f * :Sp!kevalue..,.Sampleoumber '..' ' {SY)*._. '. *. v_.*.olu*-~e,. B~n1* ~nd **1: Total. I Mi!ltlplier: .*(mJrCaC-0.JL>.*. ___ (ml)- ..011ml.,ml.Spike hardness (A) . (mg CliCO.llL} Jilf.~\\~~J...qo,L <<a.J Lls~s: 1 .. ~.if "3.1+1.... 'tQ.q ". ~*<a 0Sample.har4nts!i-(B) . (~cii¢6j11l). Measure<l spike value (MV>. ** 1 . l\\'IV=A*B %~=MV/SV.x 100 {ac:c:cptiibh: r:tugc =75.to125%) .a;; 8an111le. i1!.eaS1tte111e.ms: *Samtife numberl. Samt:ilelD TV=ND Bfank . (shiili!d'bli-=(hng Ca03'L) tbaf~7.;~* ***i~w~* L Jfi. o~o~"'J".l J 1 3 . :u., ~*tri'. ~o;;;\\ 1,f\\f Catu. t. 1~aio\\O\\-. ~ -~ t . z.:~OEOb.:ctL, l3.- 11,; r\\l*~"l.;.u), I :.s :6:<<-u l\\\\c>>utntt~o:1 Ir.t -~ j.. l\\$b.&.O\\i..n.. ii; J !J' t~.oao:t~5... :L~.wa:d1il. 1~ Sample* volume . 'lnll}.t:."h..OU 10 (!rig CitC:~L) 4il '02.s*1.Begin 1* End I Total mi. ' mt. mt I..Muttiolier o.o JO.O: f:{)-0 I to.c-{ 1:~1.1* ~.a:.:.a.,. -'I ;tJ. ... *7*~,, u.a.... l~ J zo.~I J. ~*-*r . :~o~s 1.aa!1.,I 3. y~!j I l".f.bA *2.1... ~~.oJ:-$0:01 3o ~~o J"32,5. I.z.~ l52~s-l'?>'S...;i... :.io~ l'.38.;tl. t.. ~.. 38.1h41J-l3.o~I'J Hardness (mg C:iCOJIL) µo ~3 ~*S"' laG) .. t.oo I.II 61 <DI 63 310 Note! lr.>15mloftitr.mtiSUsc:d;sllinple'mum~edi1uted.. Review~ byt f 'g\\. I -Date reviewed J O~*cM*\\'=" I Page 61 of 90 0 SOP C7 - Exhibit C7.l, revision (} 1-01-13 l l ') i J l j J J J &rdn~$ ($M 2340 C-1~97)RL = Lei mg*caco.;L J,>ag~ 42..~e 2. of.3 Tlttll11J 11ormall(V 1111d nmltli1lier determiutrlion:Titrant.Normality cbeck *' B~inEnd *. Total referen-.mi ml.nil.number number-* Laboratory co11tro/ stai1dard: Reference standard number Truev;tfµe (TV) {mg (;QCOJ/l,;J 402.Matrix spike recoyeTJ': Reference standard Sp!keval~e number (SY) (mg,CnCO..i/L) Sampl~ volume 'ml)S:im~~voiume {ml) .H>' ..J Sa~ple voiume (mt} Begin End.ml ml Be.. n End .~,fitf ml' '!3..Jq.O,\\O*O '2;.°IBegin End... ml ml ~SSt'iOZ. .x:.o~ Jb,.o.Q q.q' No:rmaifu' (Nf of El>'.fA.~(i.2/E ',' {ae~ptllble*tatige-dl.Ot8!J:~ O.OllO)pH* ~a~ SOOPO}/ SO :mlsample .Ns. lODG ~,..'4:.....,-~JCj f!al-l'ln~~(MVV % ~\\::.l\\llV1 TV x 100 Total * ~u~fiP ... J.ier. (mg,OtcoJ/4 ** (acceptableran~ml ,:;:?o.tUtO%). Ti)taf mi '{.~ .Hardness * %Rrn=:l\\'.l,ltiplieJ:.. {m.g Cad>Jj.L)({$,, J))/t(SfJ))flJ}d.0.0 lVIUI~plier 5)20*~** 'S,Pif(e: Ifardne5s,tA> - (ti!g'C:ac~>..500' sample 11arp)ies5;.{Jl}(mg CaCOJI~) Measured spi~value(M*V'). . o/.(R~'l\\!IVlSV'~*lOO (acc.c:pfabfe ra~~* ='7$,to US%) Sample melisaren1e11ts: Sample. number MV='A-B lme.C:\\CoJLl sa~J>.teyoJumeB~gm, $,id'totalaardn!!s&S.imiJJe.JD fmJ): '.nil '. mt.. '.111i MultiDner (ma::Ca:CO.JIL) t'l. *-.' "10'ex,.'IsoP C1 ~ Exhibit Ci A. reviSion o l*Ol-l3l l l l l1 jJ J j J I J Hardness (SM:a340 C,1997) . ~=1 til)el\\I., RL = LO mg CaOOi/L .. /I.itt:imi uol'm!ffiiiltzndi11ultitiJ let t1,eieni1i1101ion; Page '-13 Page S of 2 _ Time initiated I~ t8c)oJ'f "time completed ..,~.. i *. normant_Y.check 8¢gtn~ua T'otiu reterenc~ * ** sfu~d?ru, m(nil N9nna*it.r (JV) of EDTA '""*O:if.E ' pH Factor or Multiplier - (N x 50000)/ 50 ml sample number\\_.. -'nutnber .ii!>*flin~co:>O;o18o""O.~D)..-Nx 1000 _ uE'.lw;11.J Ut/Jormifrt~,-cJ>lilri!lsta11a11rd: Refer~ce-:sfandat1fTruin*al' Sample*.. nmnbet* Q:vj*,.. voifii'ne,B~gin Encl total.. i* * -CacbJIL.1 *_{-*m* ll- ml..ml'~_ll!g_,/l\\fultiplier Hardness (MV) * (mg C11CQJIL) %RS=MV /TV x 100 (11ccept:rblc range. =90 to110%) Sampletnuii:ber'. :S:tmpte *_. * ' vl'.!l~me Begin End Total ("ml ml.*. nil... ml Hardness Mtdtipl~er * (mg CaCO.slL) %RPD= {(S - D) /({S+D)/2 f} ~ 100 M.atrA;siilke tlicovery:.. Ref'ert!Jlee s.ta~(Jartl ' Spike.vnltie '$tritple 'number>~V)** 'VDltnite, Begin 'Erut f~*'CnCO.JfLl.{ml):. ml.ml.. 'I;otlilMultiplier.nil Satnple.Mrdne$s cB> ,M~asured si>ike value-(MV)(ufg:Ci!CO!!lb}MV=A-B (mgCaCOJ(L} ero e~ Spike hardness (A) .(l!lg CaC03'L) %R=MV/SVx 100 (acceptable rnogi? = 15 to 1:?5%J 10561* Sample vc"lu'ifie (ml) Begin EndT~tal.Hardness (mg CnCOJIL) ml rnr ml Multinlier z~o.. .aos --~716 Note: Ji sismlofliliantisUS!?d~sampl!:fmUStbtdliluted. Reviewed'bv: I"~I Page 63 bf 90 *.1 ...__.........,--11-r~~----'- Date revi~d.... I __ o.:.t....;;*{Jr_\\"1 __ ..J 'SOP C7 - E.'chibit 07. l,. revision 0 I -0 l -13 Page 64 of90 ' Sequoyah Nuclear Plant Biomonitoring August 02-09, 2016 AppendixD Reference Toxicant Test and Control Chart u ~tlO -en N~ m "ti ( " Pimephales promelas Chronic Refe,rent:e Toxicant Control Chart Organism Source: In-house E!'lvitonment"t 'testing Solutions, Inc;LO ~~~....,.._-....------.----.----.--..,.--..-----r---.--.,........--.-_,...-.....--..-----..-""T"""-,....---.'0.90.8 CL7 0.6 0.5 1.4 1~2 1.0 0.8 O;~OA 1.2 LO -0~4 USEPAtontrol limits(+/- 2 Standard Deviations} ,.. ~--......................................,,................. 'U$E:PAW:arning:*a11d Control tirnits(75th and 90thPercenfile CVs)4*******'**,,.** ******a**~~t.:.::~.:..:... :::~-~-*~:::....~.. ;_;--*-***~**-**-;.--"~-**-.. ~--**- ....;..;._.,,,.._.;...,.. ~**-**-:o~~-*-**~*~.1---:-... ~*~*-~** ...,-.. *--~*-~--~*~'-*."f,- .,................ ~.. *****D************************************~*******.. ******** Laboratory Warnl~g and Control Limits {10th and 25th Percentile CVs) ~.... *-** **** :Ii.*** *** ***.-*...... 11-:*.... -.:**. *....................... *~ ~* ~ ***.,........ ~ ............ ~********~******* **.* ~*......................................... 1!'*0**.. *** ' Test date 7-day rc25 = 25% inhlbition concetttratil)n. Ali estimation of the: concehtration of potassium chloride that would t:a~sea 2S%redoctiofrln Pfmephafesgrowt:hfortne test pqpulation. tentralTenderit::Y (mean IC15). warning LitnitS (mean 1c25 +/-.sA.ld or sA.75) Contrc(! limits-( mean rc25 :!: ;sJ\\.25, SA.90, or 2 Standard Deviations) l Q) Q) 0 "'ti <c Pimephales promelas -P* Envlroilm11ntal Testing Solutions, Inc. Chronic Reference Toxicant Control Chart Stoteail.U.O~EPA laboratory laboratory use PA US EPA . Test number Test date 7*day1Cu CT s control Limns s..,. Warning Umlts s..,. c:onttol limits s.,,Warning Umlts s.,,.control U111l1S C.V 11!/L ~Cll (a/lKCl) 0**25 CT~lS CT 0 Si.:10 c:T+S.1,;o* cr-s..,. .. cr+*s..,. .Cf*S~.u .CT+So.11 cr.* sA:.. a*s *.,. l OH!Ho 0.74 '2,j)3*0N6 3Q3'0H~ 4 03*04*16* sOl*OS*i6'6 04.()4-iii 7 ~UH!I B\\ os,irfo; 0.67 0;10 0.58 D.(!6* 0.81 0;10*0.61 Cl.68 om M9 o.7ll 0,10 0.7S 0,71 o.os D.60 0.Sl 0.08~.62 0.79 o.1s 9,51; o.ss a.21 D.411 0.97 0.31 0.$9 l.Q2 0,07 o;oa* o,so 0.82 o:os o.ss 0.14 0.14 ci:s2 o.ao MS 0.41 o.9.1 .0.30 o.ati 0.96 0;12 £1.JOo,so 0.90 o:os*o:62 .citlS 0.15 MS o:a~.- o.~1 o.~ o.9i; o;n 0.38 1.0t: 0,14 o.o.!i 0)19 0.87 o,oao.eo o:~a D.14 9.S4 0.1!2.0.26o;4i 0.94 o;~i O.llT o.99 0.14 0:09 a.s2 a.Iii; o:oe o:si rm 0,14 o.s4 o.~3 0,26 D.43 o.9s o.u 0:38 l.00 o.ii o,oe Jl',.53 l).87 b.os O.GZ 0.79 0.1S 0,55 o.ss 9*2?ilAf 0,97 o.31 0,3.9 1.02 i.J.12 o.os o.ss 0,Bf 0.08 a,~2. o>r& o.. 1s 056 0.86 b.'il* 0:44 o.!ia* \\l.32 0.39 1:oa 0.11 9 OS*lMli io o~'Ni. .0.94 . o.10 1).?q .. ci.70 0.08 0,54 o:aG 0.08 0;62. 0;79 o.1s o.ss (j,85 .9,2~ o,4s* o.97 0.32 0.3~ l.02 O;li 0.01 o.ss O.liS o.o.s* .0.62 1).18* .o.lS .o.ss il,a!i o.2'i 0:43 Q.97 o.32 o,!9 i.O~ Q.li u . tlli'l4'16 fr !)7~12;16* j3. Q1*19*l~ ll.14lJ;7o 0;fl1)*o.n 0:63lfo 0.0?o.ss o.es o.os 0*.62 Q.?!l O,iS o;!fo a.as 0;27 0,4,4 .0.97 0,32,o,ag*.l.02 0.10 0'.07 o.si O.ll6 9.09 0o1;3* a.so o.~5. o.56 o:se 0,21 0.44 .i).98 o.ai 0;39' J.03 0,19 o.ci:i o:s60.85 o.oa o.6t D~79 o.is..o.so. 0.85 1u1. o.411 \\).97o.32 o:a9*1;02 0.10 ~~. .'Ol!*OZ*16 0.79 0.11 b.ti7 o.s6.* 0,86 o.o~ o.~3\\1,80 O.lS Q,~6 o.ee.Q.27 0.4'l Ms 0;~2' o.~g ~*O:l' ll.10is l(\\17 18 ?9 to 'Noie: .1-d iCu " 'r*day lS"' fnhib1t10n cc.n,enrratfcn. /\\n e.stlmallo~ cit. th! concentratlcn of pota5shin1chlartd&. th;t ~ufd camft a 25% r~d~tW11l Jn Pll!'l!P!litiel ilicwtb fo.r the teJt pojiular;o,.,, q;, Ci!nt~ftiinde.11cy {mean IC)j). 5;, ~!~ndilrdil,c~;a110~, ai t.lie 1~11 )latues. t..a~oBtoiv C:on.1tol llnd Warlitng ~iiillts*

. Labor~rory:cimfroi.~nd warning !inl.i.ts wer!! il}~a.~il$\\!ed.11~hiQ lhll $(a~dardda~ietion 111 the ic,,,v<).lves corr!l'll~nd1~g1oth~,1Pt~ amd5tii p~rctinillo Q'$,, The5.G tan&

int. s.,tii:"' Standard iJ&mil~n ec1ra$ponr11ns io tile-Io!~*p~rtentifo cit. isA.ra"'o.~21. S~u'= Standiitd.de~/atliin'i:orr!itpbndlnir tii tlJe 2$"' per~~11t1l1,t tv, (s,Jj ".o.2ij iJS.~P/i. i:o1m:olamLwaroln&tim11s 5.,,. ~* sian~arcl c!.iv)~r!op cilrrespoiidiri[Ha lh11-fli~ 1 ' iier{~lil!./;\\I. {S*,n" !l~lll SA,.,;~ st~1ilari1,dij~tailii1;,correspoiitlln~,t~tli~ !lo~ pet<;iilitil~ cv. (s.~... D.4Si 'Cy"?.Coefiiit~ntof var'llition or lhe ifi> valu~~. tisEP'A.,20~0. li~i!~r~~ridirijt~il)\\<<O~'!i~for M01~odya~ib11;1y10 ~!~ i!ffbiolilTOiit<IYAppli~l4n1 u~dor,ih* ffiillll)ll.~ollutal)i'Dil<:h1rgi ~liioio1iilll) Protr*m,.fi.1o-u31*!1*ll~Mo3; us Enoiranm*nl*f ~omifo* ilf;eney;~n~ll. Olt .F -at* 1.00 r; n1 .... t! .~.;!! 0.75 I!) ii c;*~* *= o~so . c........ 8 ~ E 0.2.s. ~ ~0' -r:.;C: 0... z ~ !ti 0 20 m I!) > 0 0 t...... .... c s:: 0 cu u o I;.. fE.E cu 8 ~o ~* 20 Cl U) ~* Q,. 10 Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source:. In-house .......,;.., ****.***.** *~**** ~** *.*.***,..................... **.<'* ***********.**. .. ~............,. ~-~~ ** *~.. .... "'""""'~~~~I!!'*** **** **'****:*.. ~--";--:--,.::;-.*~*~.. *--*~-.. ~---*-*,.,-~~-*.. -*.. -*.,,-..;_,.. _. ____,,._. __.....,~_... *~**-. *-.-.,.--*-**-**-**--*** USEPAAcceptance Crit~ria (> 0.25 mg per surviving,fai'vae} Kentucky Acceptance Lh:nit ( <30.0%) ~..... "*"."'. ~*******---........ ~............. **-***********............ ol!** *.* t:onttol lleprodu~ion, Coeffident of Vari;ruon (CV), or Percent Minimum Significant Difference (PMSDJ* ?Msb Is the *rninim um signifkaiit differ~nce betw~en the control anci treatment that can be deda17ed; statistically stgriificant Central T~ndency {mean ControfGrowth, OJ, or PMSD) -COntrol:timits' tm.eari Control' Growth, CV, or PMSD +/- 2 Standard Deviations} e Precision of,Endpojnt Measur4aments ).... Plmepha/es prome/a$ Ct)roni~ Jleferenc:e Tt.Jxitan1 D;;lta Test number Test date Control Sun/iva! Control Mean '1 G~wth. Cf* 1 2 3 4 s 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Note: {%) 03-01~16 100 03*02-16 100 03*03-16 100 03-04-:16 100 03-05-:1;6 97.5 04-04-16 100 04-05"16 100 05-10-16 100 05-17*16 1()0. 06.-07-16 100 06-14-15 100 07-12-16 100' Q],.,19-16 ' 100* Q8-02-l6 100 {rrig/larv~el 0:56~. 0;1~~* Q.f.f71 0.5,1 0Ji30 o.451 0.647 0.541 0.,5()7 0.421 0;<155 0.5.78 '0.774 0.570 for Control-Growth' lmJ:IJarva:ei o.66i 0.664 .0;646 9.~43: <Mi~ -0.611* O.!'iOS 0$7 o.s19 Q;5$ 0~569 '0,!?84 o,ssl CV= Coefficient of, variation fot conrrol'growth *. lower CV,boond.!jetermined byJJSEPA.{10"' percentile)"' :35%. Upper CV bound ~etemii11ed by, USEPA (so"'~entiie) = m% MSO = Minimum :Signif~nt Difference PMSO = P$'cent Miormum.Slgnificanti;>iffefl!nce Cv 0: <91-J, for*Qlnirol ,Gi:oWlhC\\11%1; 7E l.'3. 4;6 5.a s:Q 3,1 4.S M 5.z 5;!1 5.3 12.3 '6;3 1:0 6.4 L$ 5.9 5..0 5.8 3~2 . 5'6: s.s 5.9 .lQ.l 6,:a 3A* 15-,0 MSI> l'M~n ct (%) forPMSD(%} 0.07 11"8 0!07 8.9* -10;4. o; ,f3~4 llA !),()$ ~35. li.., o:os. 8,1 11.2. O.Q4. M 10.7' 0.11 17._6 11.7 O,QG u~a 11.7' M4 8*~ 11.3 Q.06 ~;tU '11.6 Ci'.()3 7,3. ll.2 .0.08 ~;i* ll.4 o;U i-a.-s. 11,5 OJJS a.~ 11.3 PMSO Is a meawre.ofiesfJirei:ISion. ThePMSO is the minimum p~tdifferencebetweenttiecootrol a<:idJ:reatmenhhat.caitb~. det:t~red statisth:aUy slgnificaotio awiu>ie-effiuenttollicii:y test. Lowei"PMsOboundde:te~m\\ned by 0sEPAJ1(1"' percentile~ "'12%. UpperPMSO hound,d$termioed by:USEPA {9(l1h petcefitile) = 30,;,; CT= CentralTendam:y !meai!.ControJGrowih; CV,l>rPMsti.) USEPA. 2000. Undetstandiog :ancl Accounting for Mi!t!locl Var\\al:llhty In W~ole Eff.lue!'lt ioxlcitY.P,ppl!cat\\Qns.1.Jnoer*tl'>e~National-Polturant Disi:l~ame.Eliininat!qn Pn:>gram' EPA-8~3-R-00-003. US Enlilronll)ental Ptotectio.o.!\\gel)cy, tincinna,ti, 01:1.. ' USEPA-2001a, 2001b. Final Report; lntei:!aboratory' Vartabllity Study of E:PA ~hort-ter.mCIJronic*and f\\c;ut.eWho~~fflt,tent Joxl¢1tv Tajty\\ethOds-; \\lo1i11rtes f._;ao9l. Appendix. EPA-821-B-01-004 and EPA-8.21-B-Ol.005. !JS Enviroorrieotal Ptotectio11 A(len% i:Jncin0a~i; OH. ~--* ~,. Page 6Bof90 .O*. a* Page 1 of6 -~,, Pobssium CJdotide: Chronic ReferenceToxi<:~ht Test . tEP'A~82l-'R4l2.0i3. Method IOOJU)) ,i Species:*. Piniephales primtlikis PpKCld~ Tem:Number: 14 Stnck r~n:. so g K~llL; p ** DiSSolveso... K.Olml4..Milli-0\\vater.. i:t 15* 18. '.98~ '983* 9S2 .igQ(t 1000* 1000 In.;.house.cilltill'e , ~~(>\\4l~ '-~?<? ~ Ot**~~..,~, : b\\O\\ .*:pB>= ~.~b.. .T:~'tttre,: LS-.\\ io~11 lliL.. 1Jii,ilyfeeilil!g ondrenft1Jiaf iiJfiJ111iation: 'J 4 ', 7,* Page 69 of90 li m.* 1099. Iifoi1bator number and .* sbeJftoeation: D,rymg l1tfomu1tionfor weight d1itf!n1iftiatf on: Date/.Time in c>ven: . Initial *oven temperature: Date / 1iime oU:t or oven::. 'final QV,enJerri eraiure: Total:diylngJime: 'i.. Test. ir1itiatiori, tenewal, or termination ' *<::hV MHS\\Y batch used SOP ATil=ExhibitAT2U,revision 11-01-14 l 1 I l I ~i"' **:~;*r*;i ) ~*~~*~~~~~Sol~::.. Species: Pimeplzales promelas Day l 2 3 4 s A = Pan weiglit (mg)a Tray color code:: _I q VCo dil Analyst: J1'- Date: "-l:;?x').\\!11 C = Larvae weight (mg) = B -A Hand calcuL'!ted. _ 1 Analyst: __ _,,,V\\~--~- 11 Survival and Growtli Dma I J K.. L. IQ* 10 JO 10 JO to to io io 1 ' ** in 10. lO* .Jb ( 0 ' (Q l t>, I.t>.. ft) ;. ( {) lO* t 0.. IQ. IQ (C (Q /'Q t*o* tp. LC.* tq * .ltj lO* liJ. JQ, IO lb to to lQ* lO 10.*19~ l(:) IO 'l0; ~o d.() Jo. ,Q, l(:). fO. (0 ltl* I(.): lb :lt).t\\l JO lO ro l () t3.ol s.63 t&.S\\ J1,,.ti. 1~;4!~. J3.11 l4~tfq_ ls.le I~~- f:~ J~.t~ tts.43 Comment codes: c-= cieL'J"~d =dead, fg. =*m~~ 'le =.idll~ ~ = ilii$.sing;,.~ =;;,gl~k,,sm,.;,, un~ally smalt lg = unusually large, d&r== decanted.artq :returned, w =:wounded. Comments: Page 70of90 0 ~ J \\ &Zt~~~/ Page 3 of6 .Species:* P1m~pltatespromelns. Ppf<CJCR Test Number: 14 J:'. .* ~* c=r.ame-weigJir<miJ>"".B,....A Haild.~kutaitd;;: , Ab~tysl! . *.J... Average w~igbtpe:t i~itlal. Pcrcecnt SimvivtJl altd Growth DUia 900 lllP KCl/L .Q R:.. *... s* T u v w x 10 JO to: to l() JO m io 10 10 10 lO: *. ro (,'Q: JO 'rt) ; ' t b *- ll:)* 10 lO ; tO : -t {) I ti: f.D ID f () to' *iO IP "19 lO 'l;O*':{;O' gaJ ...... ***-****.. - *~*~ s~ .~ -u. 1., ls> JO 10:: -6 e -:r-r-....... (p .*. if.); l () ~ ... J;. b ~ b l'i~~- ~0.1 1 ~~St ~&.ftt.l 11.~- 2*1~6'~: *J-~1~ ~& .. 1'f fqlf1 l'l,1 J1J!.f 1g.£lq iltiniber l)(lnr'v:lc (r,llg)' ri:ifoction* i ~"" trom contr0l o. ~ u.. S\\."\\ l {%). . Con111iditS:. Page 71 of90 SbP* Ari] -Exhibit A T2 J ~I, revision J 1-01-14 Species: Pinzepflalu promelas tl 2 3 ,4 n '= Pan + Larvaeweight (mg)* An;ilyst: Alb Date: fl&-11-li c =Larvae weight (mg) ""B..,. A lfaod* calculated.- .. Analyst: .~ Weigflt.pcr inl~inl number. oflarvitc {mg) =CI Initial number oflarvae Hand caku)aled. xl Analyst: __ __ _,_,lP----- Average weight per ~nttfal Percent number of lll.m1e (mg) .rcduc'tion from contr:(!l .(%) . JOSO:ni Keitt Y Z ' ' AA *.BB, 10 lO lO 10 iA.. .,~ ~"~ g1A. 'ti\\. b '..., "G\\,. ' g Sig ~ '~ ~~" -~ -~ g* 3~J 4~~ *fl;* 0 0 '.\\ lo s~ 11* .*,al 31. *tti r;. \\ .~ '-l~ '1.. ~ \\ ls.6'-/ I s:Jt> Jfi.tf\\. IS.b"': Comment codes:- c = cl~ar, d = deaQ. fg:=; J.ilngll$;k*= lolled,.m = mj$~jµg, * = $irik. sm = unusually !:lmal, *.. lg*= unusuaUy large, d&r=decal'_ltedand renrffied, w=*wounded. Comments: Page 72 of90 Pag¢4of6 Pimepha/es prom.e/Qs C:hroolc Refen1nce T9xlcanfT~st EPA-8Z~~~*o2-0l3i l\\l'li!thod 1000,i);: .Quality COnfl'<il. V!!rlfl.Cl!tlon of Data Entry, Calt:ul~ttons; iincfStatlstlcai ~na1Y$~ tau~*!Dhef!_. _______..,.... __ __.'4_ EtMronroenfal'restlng Sollltlon$, Im:; T1!$t ifQ11s:. _______ ._11_u_gils_**_1_0J.._o_* ~... 2_0_1_&.* 11* Coiltri>l .. *B. c o E* !loo F G H. ~so )_ K l M. 600 N p Q 750 R s T u ~OD V: w x y IOSO 'l 1111 118 Ounn-~lt's MSO vaiue: PMSDt 1&11ahiu.n1btif~I * JJ(illnvmbn~; A.*~.tft-~J4i'IUihl) a,.h'f'l.*.laf'ffa ~~'~ 1it¥11 ~~!ib\\(~111 ~rwu't!ilif\\l(~*)~ VJ'tl~1/J!'ivM~.ttC.* Mt~nwat1ht/~ crifffldanfdfvirl.at)Mt W.ti~i/11'1':t&l~li'ibor~~ M**U'f~'{" Mo!'.w**lf!i/JnltflJ ~ffltftn\\of~t.l~t1on *hmn;Ha1h1tif4nhom hA rttt~~t6lt&ttH.(n11J ~kMb*ro.tt~rmj_J t'i!t""'~'p.f"'~ *.. fmtf~rl 11~1'*to:tf1~ mr ~lrol.tHt*. 10 10 .13.0l' 10. 10* 1S*.S!'. 10 10 .15,51 10 .. :10.. 16;12*.. .10 10.: ..,)5;96' let .!O 13,3!1 10 10 . 14.49' 10 10* lS:lB* 10 10* .1S.4o* 10 10. JS.17* .10 10 !6::U. .io* 10 JG.43' lO' 10-1s:oo:* 111* '10* 15:78 10 10* . 15.73 10. 10 "1S.9J: 10. '9' '15.14 10. 10* -16.44* 111 10 15.28 10: 8-16,23-10 6' 1G:4S, io* G. 1s.os*. 10 s !4.J9 .llL G l6':lG*.. 1a r 1s.s4:* 10 15.EQ 10* l. 16.09 10 . *is:o1: 0.0414. MSOo 11.J; PMSD" !Wftlht1Jitnu1 ffl). fmll 18.87**. S;M 0,584 o.584: Zo.9,S SA2 '0S42 0$1p u. 0.542' 100.0 o.na M Not a~plf;abie, 2lil3 . S,82 *. o.se2.. -l).58~> 2i;l!4 l;.7~ ~ 'g.572, o;sn* .. 21'41' SAS,.... .O.S45. 0.545. 18.85-; SAG O.S4& i.8 .. O.S4G. 1o0.il 0.546 2.l'i* 19.77* .s,2s o.S2S 0.!;1!6; o.s.w. 4.2 21.43,; s.65 0.S&S O.S6S* 20.n .4,71 0.471 0.471 20.ss.. s..a-0541 !i.53! 8.0 0.541.. io~.o o.sn* 2l.S6 S.64 O.SG<I D<M u.o: 21.99 S:S6 o.ssG .. o;sss:: .19.$0 4.60 IM6(f OADO. 20.19 4.41-0<4~1 6,6 'D.441. 100.0* Mn M* 17.2 20.ee. S.1S O.SlS o.4n 1'.Sl.S 20.64 4,72 0.472. o.472*. 19.9$: 4.81. O.Sl4 0.481. 2.t.54*" s.io o.s10, 0.518 8.l li.S10 92,5. 0,477 .S'.J '16.4 !JI.Ill 4,54 0.464 o.464 ~G.74* 4-51. . 0.564 0.451*.. l~.19 .n* 0.457 0;27-J '17.97 Z.S!' o.4al 0.4.80. 10.7 IJ;l$;- 51.!i !UR 4.6 s~,g 17.!4, .2.75 0.550 0.275 .18.94' 2.sa*- ,o,430'* o;:>.SB 16.4S 0.61' o.s10 0.061* .16.78.. :... us:.* 0.590 05?3 O;Ua.. ns 0;09~ 48,G i1:ss* l.56. 0;520. 6.7. 0:156* B;t;D* .*1s:G4 O.S7 0.570 D;OS7 Mlnimum_s11niffcani Differ~~<* P~rtent Minimum srantfi~**t Dltlereoce PMSb lj ~ motiu(t of la1t p,..tslon, ihe_ PMSO fl lhb minimum peKtnl dlfferen{e '1e!Vleen !he oontrol.*nd 11.. tmenl lhat.Clln b& d<<ll.W st*lfsllCllfirsltinl~nt In a wh<lle cfflUeril w><iCity L!tiL LcwerPM5b bouf\\d.d*larmlri6d l:tl. USEP11.l10th fH!m!llllll!I "121l. Upper PM~!> bllllnd de!ermlnl!d bi> us SM (SOth petcentllo) s 3W. . low*r ~*d up""r PMSO bGunds Wl!fe determined from ihe 101h ond 90th p*rcentli~. respetilv*Jv, of PM5Dd1t1 from. EPA's WET lrtlttla bor1tory Vanabiltt1.5tudv (USE PA; WO la; USEPA, 2001bl. USEPA. 2001,, 200lb. Final Report: lntorl*borotorvVariabllflY StudyoHPAShort-term Chronk; and.Acu!o Whol1 EfnU.n!'fo>diltyTe*t Mothods, Voltimes 1and2~Appandllc. EPf\\>ll21*Mll*Ol!.4 *nd EPA'321-/Hll.Co.5. us Environmental Proletllon Agency, CJnclnnall, OH. ~* !!~~~~ \\,~~ ~,~,~{* ) ~:::~1;:-L-GSQ~~~~ s1ar1:oate: ~2016 End Date: 819/2016. sample Date: * comments: Conc*mg/L D-Control 300 450 600 750 900. 1050 1.0000 Loocio UlOOO 1.0000 o:sooo 0;6-000 0.1000 LarvalJFish Growth,and survival Test-7 Day survival* TestJO~ ppl<CICR:... Sa111ple,!t>; ~EF'R.ef:r"oXk:an!. 4b IP: :ET$*Enlllr.. 1'estlng sot. Sarr;p!e lype; t<CL;-P.otasslum ehloride. firotoc:OI; FwCHl'M!PA"821*R"02-0'i°3 Test~pecle$~ PP*.Pirile?.hales:prert)efas; 1.0000*.).. 0000 1*. 0000 :;.oooo 1.0000 .1.. 0000 1.0000 *:l.0000 ~.oooo *1.0000 0.6000 : :o.sooo 0.2.000 '_0.3000 1.Q()Oo 1.0000 jJJQOO 1.0000. o-:ao?Q O.OOQO 0:1000* Conc;.mgtL N11111t1er Total Resp fllumi:itr D.,Conlrol 300' 450 600 750. "900 "1050 1.0000 1.0000 *1A1;io 1.4'12(1* 1A12p* .q;ooo. 4 1.0000, 1.~lOOO 1;4120. 1*:4fW 1'4120 O:OOO \\l 1.0000. 1.0000 1.412'> 1;4120 1.4120 o.ooo 4* 1,0000 1;0000 1.A1~0 1;*;i:Zo 1A120 o;ooo. 4 0.9250 0.9250 1;295'1 1.1071 1A120 11.347 4 o.5750 o.s7so o..asos -o,78s4 <i.8sr:;1 5J!47 4: 0.1750 .0,1750 Cl.4217 0.321!;\\' 0.5796 29-JiB:t 4 1~coo 18.00 1iu:io 14;00 10;00 10.00 l(l'..00 Hi.()Q' 10.()f.)* 10.po. ~g2g Q 40' ti 40 0 40 A 4o 3 40* 17 40 33 40' AUXIiiary Tests: . *Statistic .. Critical Skew.. Kurt * Shapiro-Wilk's Test indicat~ non-n.oi:rnal 'Clistribution (p*'<"' O.Oi) 0.83328 .0.896 Eguallty of variance cannot be confirmed HyPothesis Test*t1,tal1; 0.05} . NOEC LQEC.. ChV .TU .steers Many-One Rank Test* ..750 900 s~1,584 Treatments vs D*Control Ma.)clmum *Likellhood'*f'roblt Parameter* Value SE 95% FtduC!ar Limits eontrol..mi1,.sg Crltlcai P*valutf.. M'u Sigma Slope. Intercept TSCR Point EC01 EC05 eC10 EC15 EC20 EC25 EC40 EC50 ECSO EC75 ecao EC85 EC90 EC95 EC99 16c4547 2.45609 't1.640S~21.2.686.*** O" Q:OSSS 9.48773* 0.99948 2.~7.Er 0;060.77** -43,?a.5 i2694s 1s11.'os3 *z9.:;a5 * .Problts mg/L 95%Fld1.tc1arL1m1~* 2:614 665;892 580.'9.01 719.902' s:35s 732'522 ~62Jl69 777.202 3.718 770.725.'t10.4~B 8?0'416 3.964 797,~2 (43J'~4i 1:134.25 4.158 61S.563 i71.149,854-~ 4.326 839-059 794.7:j'i 87;2.396 4.747 889.993 *853.898 923.$73 s.ooo e22.1i1 aa8.6s1 ss9.741 5.253 955.3138 /920.64' ~900,43 5.674 1013.$8 97*2.105 1.077.81 5.642 10.37.* 36 ~2.01.3 "1.11:1.5': s.o::i& 1066.-03 w1s;2s n5Z.a6 6262 1')03:2~ 1044,63. *1201;71 a.645 11s<F7 1q~a.11 12s.s.o9 1~s26 12713.91 1174;93 ;147s,4Q, 1.ll.--------'-'--------=----. o.9 D.B (l:7 ~ 0~6 ~*. o,:.O.lr ~i,o.4 .0;3 O,:!: o,1. j o.~+..-r;;....,;,,-.,..,.,.,..,,.,,-,..,,.....,,.,,,_..,,,,.i '1' 10 too 10~0; oooo ~o~m!:J!L Dose-Response Plot. s. ~~~o--~-o-~----:""",,,.-~..,...~~~~-,---,, 0.9 0.8 .. Q.7' > ea.a** ell 0.5 ~- Cl 0.4 "'*o.a 0.2: 0.1* o..... -~_,..,,-,.~~,.......,~--.-~~--.-~~.,,----1 0 E (i o* 0 0.,,. 0 "' 0 0... Page 74 of90 ltet. 3' ~,., .~~~ ... ~*~ 'Sfart Oat~ i!f212t1\\5 'Eru:tl_)atii!.. si9120~e StlmpleUale: .COmmei>?s: O'<lontror :-0;5540 $00'* 0)5450 4so 0,47.10 600 0.4600 7so OAil10 900* 0,;>;740 1oSO OJ~S-10 0:5420 a:s46o (l,5419 .o.4410 o.s'ioo ,0,2590 .o:Heo StatistiCal Analyses 'Te!ltJO!'* 'P~ClCR sample 10: REF-Rer Toxlcanl L;ab(O:; Et&-Eovir',~t!i19 Sot, saritpleTypi;: KCL.*Paiassium*ch101ti:1e P,Ptoeot 'FWCHR-EPA-821~R-02-013 Test Species; 'PP-f'imepbilles promelai; . 3. . 4 o:se20 o,5720 0.f!~ 0,5550 tt5640 0.556il 0.5150 0.4720 .0.4640. 0;4510 0:21so. o.2sao. 0, 1580 !M)5/'b rrariSfOfrll::Untr.:instol'l!'lad* '1-T:iilac:I. Isotonic cc:inc;stlt . llilDi:ln 'N.iMean *Mean Min* Ma" CV%.. 'N.. t-SW p.qonlf'Ol 0$(00 '1*.0000 0,5700 oo;S4ZD* 0,!ig.(O 3A02 r '300.* ct54eo 11;9579 {);&~SQ !Mi280 0.5550 '2:770 4 1.'194 4~6 o.s33o 0.9351 tt533o 0,4710 9.5i>40 7.-958 4 1.84i "fjQO 0.'4726 ~;e2li1 0.412:0 OA4iO* *o.s1so 5.6.ia 4 4.a77 ..,50 U.4765 O.B36tf 0.4'1'65 0.4510* 0,5100 s.349 4 4;653* son ~.~7,4,0' '0,4e07 02740 1).2580 0,21!90 t626 4 '!i:lso D.0980 tf:171,9 b.o&so o.n57o 0.1/360 48.. 631 -a Avi<llia!!Toms* Stalistil:' ~PJr9-'l/V!lk's Te$t.1!ldJ~~ !'lorrn~Jdisttjbu1iofl *CP > 0.01} 0;97576 Baril£.lf.sTest lm:!ieates.e9uat *vanancii!s:!e.. o.s1I .3.Zls4s liYi>QtbesisTcistf1*tail,O.Oof** Ourinett's Test* 1'mll~lln1s 0-Cooliol . mgiL. SD NOEC *,l.OEC. ChV:.* TU. .MSou*. MSDe 450 eoo 519,!>15* 0.!)474'3 0.063:£ Unear lnterpolallon {200' Ros~plss) 95% CLISXPL.. Skew *... CiitiC31 .MSD Mean 2.'360 0,0474 9,5100 ();5450 2:350 0.0474 0.5330 2;360 0.0474 0.4743 2.3W 0.0474 OA74'3 0,2740 o.n9ao Critii:al Skew o.asa '"0;5272 13.2767 MSB MSS.. F.Prol:I O.l>Q754 0.000!11 *s.4E-04* ICQ!; 1c1c IG15 IC20 l~ IC-40. lCsb 351.92* 50'1~06 .513;e3 763';67 1e$.ri:Z S49o 891/re 92.SS* 46.as' 1~6 38.:97 125.98 -579,-:;3 0.0791 1104.90 Stltl. f3 ..0~7:.99 459.54. fiS.4.<!3 1:~226 484,61 '7S1;91 -s~7Z44' '7ss;:1 soo~69 -0;1aes as1~s stt1:36 o.oz.es ais.12 *004.es o.'1492. 1.CJ*,,,., -------,,--------. 6.~!f 4.82 4.~ 0;9' o.fi* 0;7 ii: 0.6 c g_ Cl~ ~ 0!4*, O,~ a.a o.~ 0.0 0o=;::;..,.._,.....-,....,..,....-."-...... ~~~ D 1500 Dose* mgit. 0;7.,.,...------------------~ o.S =- ~ f0.4 C) ~Q,3 c .. ~ (1.:2: !);1 Page 75 of90 14aa. *q.os 1ev:el of signll!cam:e N-Mean 1.0000 0;9579 0:9351 0.8320 0.8320 0.4607 0:1719 Kurt 0.54083* df 4,15 i ~¥ } .J "'~.. __...! .J En*lr<>n"""'**ln.,,f"ll~°"IJ\\<.. Species:.Pimep/iales promelas Daily Cltemis.. Anal' st Concentration Parameter pH (S1J,) DO(mg!L) Conductivity l mhoslcmi DO(mg/L) 300 mg KCIIL Conductivity { *mhos/cm} Temperature ("C) . pH (S.U~) DO (mg/L) 450 mg KCl/L Conductivity CP.mhos/cm) Temperature (0C) .PH(S~U.) DO(inglL} 600 mg KCllL Conductivity lOS/cni) Temperature ("C} pl!(S.U.} DO (ing/I..} 750 mg KCl/L .Conductivity {µmhosfcm) Temperature {?C) pH(S.U.} DO(mg{L}._ 900 mg I<Cl/L conductivity ( mhos/Cirl) "Temperature {"C) .pH(S.U,} DO (mg/L) 1050 mg KCl/L Conductivity Otmhosfclri) * Temperature (°C) Page5 6f6 BpKCJCR Test Number: l4 "'Temperatures performed at the time of !<:st initi:ition, rene\\v.tl.or tcmiinati911 by the analyst ideniified in tlu::D11ily Rcnew11! info~atirm table loCl!led im P~e I. ,....;,...:, ity and hardness performed by !he,arialyst idcnlitied,on d~e bend! sheet sp;:clficfor each aoalysis,and'transcn'bcd to this*beneh,shect.Q:y; *~ 11:.v1..... 1rv ttt~!*Vf:. r..*"4rµ Page 76 of90 SOPADl -ExhibitAT2Li, revisip11 ll*01-l4 Cl Q' ,,._....,,.'"': i} 5~;~~i Speeres: Pimef!}ui~ proifzelas Page 6 of6 PpKCJCR t~Nurnber: 14 Day (J\\nalys~ideni,ified't'or each day~ perfonned pH,.D.O. aild conductivity m¢aiiurcments only, i Concentration. * ~an,lpieter CONTROL . p}l.{S;tJ,). QO.{mgtL) ~11du'cJivity f mhos/cni). "*Alkaltoi!Y . <m CacO:ilL> 'l!IJa~dl:I~ {moCaCoJIL) temp~atu~ C'C) rm (mg/Ll 7.9 300 mg l<:Cl/L.. -Condn~iivity ti'i_. 7*1*: L*~'~=m=hoS'/,=*~cm~)~;~--1~D::__..:...._..,.. 450mgKCllL 600 rngl<.Cl/L 'f~perature (°C) DO(mg/LJ Conductivity (pJTihostcm} Temperature; ("C) pa (S,l!,). . D.O (mg{j:.l Conductivity mhostcm *, .;Tcmpl9'ature .*<aC} p.l,J{S.µ'.) .DQ (mg/(.). 750 mg KCIJL., Conductivity ~=*~ho~~~cm~):..._~-'-1-.;;;..;...-'-"'---' 900 mgKCl!L 1050 rngKCJ/L "'Temperature . (°C) ,pH(S;O.) . PO{mg/L) ConductlVity cJimliOsl.em) * Tempe~ture t"C) .Pll(S.U;} J)(){mgli..) C<>nducfulity c rtiiiWcm> Te~perature <°Ci *.. . foifial ..Final Jnitial Final Final lnitial Final ltu!>>Ot~t . ~~~ turespeifcrm~.at*lhe time' ofiestinitia~io11, ~newril or t¢fminatiori ~Y th~_ analyst. identified !"'!he: Qaily_~~ewal l~fonnaiion table locdt on Page I. >;.llru!ll!fft!i and*hardn~ perfonned by the analyst 1de11td'icq 0111he bench sheet *specdic for each :malysis:and transcnbcd co*thtS bcnch sheet by: ~ Page 77of90. SdPA121-ExhibitAT21.l, revision'll-01-14 'Ceriodaplinia dubia: Chronic Refet*epce Toxicant-Control Chart ~. Environmental Testing SolQtions,.tru:. '1 --u = z ...:l - CID in l"4 u ~ ~ o= "'O I 1.14 USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1.10 l.08 -..****~***.~111._******.. ********~~-***************:**o*Ci.*~**********~*~*******-.****~*~090*..,. ~********-"****** 1.06 l.04 ....... o**.****ai*a.~~***o*******,.*****.*****:*.~*11***..-~** .************~********~~,.****ca*.*... 1.02 2.5 USEPA Warning and Control Liniit$ (75 1h and 9o' 17 Perc_entile Cr's) 2.0 1.5 1.0 ~. ~*..... * Ii e 0.5 -**"-.***-***.-*.-..:...~ -**~ .... **-*~-*-*---*.. -**-"*~**-**-**-.. *--*-.:.-*--... *--'***-~:.----~---*- .e:.................... *!7 ****,,........... it'~.*.~ ** ~-.~..................... *,*:--*** 0... 4'0**it....................... 1.4 1.3 Lab~ratory Warningct.ndControl Li1'i1its*(I0 1# ahd2S~PercentJle.CVs) ........................... **~*-:*~. *:***** Qll(f(J ******.*,. "..................... ti;******,*~** *~*!"*****~******i.~*** ~.*:~* l.2 ~_,;.,._,;,~~.--::.... :.- -**- -*-'-~~-***-***--.. ~-*-**- ___,,_*;--... ~**1-***._.:_.,;;_ *- 1.1 ......,~ * ......... =-...* *.............., ***.. *.-==.* ** 1.0 0.9 7-day lC25 = 25% inhibition concentr~tion~ An estimation. or the conc~ntratfon of'sodimn :chloride* that would cause a 25% re(luctionin C?riodftPhnia reproduction for the1testpopuiatfott Central Tendency (meaii IC2s:) Warning Limits {mean IC25 +/- SA.JO or SA 75) Control Limi~s (mean IC25 +/-SA.ls* S A.'90, or2 S~aµ.daroDeviatio~) Page 78 of90 2 3 4 5 . 6* 1 8 !J* -10 -11 12 13 14 15 16 17 18 l!l 20 05*03*1!'1 04*07*1S ilS*OHS OS.OMS ON<\\*lS 08-114*.iS 011.iS*lS l0'06'1S 11'!)3-15 12,98-15 Dl*i,2*16 02*09*16 oi-ofa-.1& Q~*OB-16 o4.os-1a U4*llS*16 QH0 b6'07-16 DHHG 08*0H6 7*dilV \\Ch (f: s f8/~ No~ll (g/l NaCl! 1.05 l.05 a.01 ~.OG' 1,(Jri o.oi 1.0.1* 1.05 0:01 l.08 J..05 o,Ol. l,~6 i.07 0.01 !;oli. i.01 .0.01 1.1i*1 1'07 0.\\)1 1;06 l.07 '0.01 1.07 1;07 0.01 J.01 1.07 0;1)1 1.06. l.01 Q.Ol 1.06 1.07; O.ot 1,06 i.07* o.~1 1.0.1 i.07 M1 1;07 1.01 o.(lt., t.ciG 1.07 (teil 1;06 1.07 0.01 1.05 1:0& a:Ql 1;06 1;06 0.-01 1.07 l;06 O.Di-Cer(odaphnfC'I d.ubla Cbroni~. Reference, Toxic~nfControl Chatt State. and US EPA control. limit~ tT*:2S CH2S 1;04 l.08 1.04 1.0ii i.05 i,oa* i;o4-1.011. i.os. ~-QI!' 1.05 1.oil 1.0S 1.Qa l.OS' 1.08 1;05 l*Oil* 1.05 1.08 l.OS 1,08 1.Q!\\ 1;09' l~os l.OH l.OS 1.08 l.OS l.08 1.05 J:.()8 i.DS 1.08 1.0S foe t.9$ l;OQ, t.05 i:os SA<*** ll.O!l. 0;09 n.o~ 0,09; D.il9.* 0;09. ii.og MB o:o9 o.o~ 0.09 D *. o~. 0.09 0~09 0;~9 0;09 li.il9 0,09 0.09 l).i>ll to_fiotatary Watillng liin1rs er' s~.* ~. ~:+ s~;o o.iJil US ti.!i~* p:r; 0.98' i;t5 0.98 1.JS o.911 1-.1~ ~.98 1.l~* 0*!18* l.J:S. o.!ia us D.98 l.1$ 0.98 US* o:~a 1:15 0.9.ll 1,15: 9'.9!1 us ().911 1.15, 0.98 l.iS. 0;98 L1,s li.98 1,i5 0.98 1.lS 0.98 1.1s D,98 US a.ill 0:11! o.i~ 0.18 0.1.s 0:18 0.18. o,1s D.18 0.18 0.111 n.18 0;18 o.1s 0.18 0.18 0;111 o.111 0:1s 0.18 laboratory cantro1 't1mns cr,s~u ct+ $,1.ii D.88 i.24:* 0.88 i.i4 o.8~ l.i!i Q.88 l.is o;as 1.2:!;' 0.118 .l,.l!i' 0.88 l.25 0.88 1.25 o.89 1.25 0.89 1.25 0~89 l.'l5 0.89 1.25 U,89 l.25, Q.~9 1.2$ 0.88 1.25 0.88 1,25; 0.$8 l.25 ().88 l.24. 0.88 U4 0.88 1.24 USEPA* WarilfnaUmlt~ .s._ro <;T*SA:n cr+s,.,,1 IMS D.$9 l,.5'4 o.66 0.48 D.59* 1.54 o.e!i o:-ta o.sii 1.54 0:66 .D:4ii* Cl.5!! 1.?4 0.66 ~.48 o.s9 1.. ~4 0,65 0.48 o:sg l;SS. 0.66; OA!! Q.59 l,~s o,6$ o;4s P,59 1.54, 0,66 0.48 o,sg us o:&Ei !Mli 0.59 1;s!i 0:66 '0;49 o,s9 1.s.s C).66 0.48 0;48 G.48. o.s9 l;SS 0.66 iJ.sg 1.s!f 9,~6 o.S9 1.ss o.66 Q.48 0.59 1.55 O,Gli 0;48 o;s9 1.55 o.~& 0'48 o.~9 1.54. o.&6 0;48 .0,59* 1.54 0.66 0.48 o.. ~9 1.54 o.~ o.48 o;S9 U4 0.6& Nille: 1-d It;,;" 7-da~ 259' lnhl!11tlontonc1nti~t1011. An*e&timat.lon of th~ concentration of sodium chlorida that would c;usc *;, *2s%*roductlon In c:~nciilaphniii teprodui:tion for' th& te~l'pbjmlatlon *. CT~ tenttal tendency (mean 11;,,j; $'" Standard de~iation or tile lc~J:wfue&. \\iSE~A c~ritrillUmlt~ 91 cr~s.,..., CT+s~,_. o.40 1:72 0.01 MO 1.7i o:oi o:lio: 1.72. 6,01 9Ao, Pl 0.01 iMO.- .l.73 0.01 0,40' l.73 oJli Ml i;73 om: o..;o: 1;n. 0;01 g.4l 1.7-3 O.Dl d.41 1.73 Mi ().41 t73 O.o1 0,41-1.73* O.Ol 9.41 I.73 Q.01 0.'41 i:J.3 (l.O~ .{J.4t l;'ll 0.01 0:40 1.73' t'l.01 OAO l.73 0.01 o.~o l.72 0.. 01 0,40. t.n 0.01 MO. 1;72 0;01 , ~a!J,orator/Control and Warnln11 tlmlts Laboratory.control-and. Woinlng limits wete es1abl11hed U$inil the standard deviation of.lhe lCn value~ corrospondln8 to the lOtli and ::iSth percentile cv~. These *~*n"guare mor~ shingenttiiii~ ihq.c~nirol end wzirp;~g-llmfls recon\\lllended.by*ussM far the test ineiho'd Md 110dp0Jn1; s..,. = Staliililrd d.O~lalil)ncorcespond!ng.to tho io** percerit!Je.i;:V,, (s.,,Q = o.oli)' s *. 11 = stondard deilia1ioncorrespo11dlng.ta thn 25" l'erceritilo CV. (S,.n = 0.111 USEPA Control and. war11Jna Limits s....,.: $timdar<f rlevtaticin correspondfng,to the 75** iiercentilc CV. (s,..,," o.<ISJ SMo"' Standard de~l~tlon corre1pondlng.to the 90'" pe1ceritil~ CV. (Sua" 0.62) r:*l= Coeffcclent of 1r.11iat1on of tile IC15 values. IJSEPA, 2000. Um!~ntandlng and Ateauntlng For Method VallilblJlty In Whole Elfluu.nl To~ictty Applications Undet the Nat101ml Pollut*nt Oischa*B" Elimlngtlon Program,. EPA*ll33*1\\*00-0IJ3. vs fnv1ronmenlal Prottctlo11 Aganty, i:lncinnatt,.OH, ,,1..-- 'f :;* Ceriodaplinia dubia, EnlllronmentaJTeStlng Solutioni;. Inc. 'Chroni~ Reference Toxicant Contttil Chart Preci&.ion of Endpoint Measurements 40 c:: 35 .=: - ..... ~ CJ = ~ 30 -e e 0 ~ Q. bf) 25 ~ c:: ~ *r: ~............... -~,,.....,~...... 0.... *'!*..-**'*....... !'***-****~ ":-*~. Q. 0 20 ... ~ c 0 0._, u 15 --*-,~---~*--..,,-~,*-~~-"'**-~"-***-~*--.;..<_,*,.-~----*~-***--.~-*~.. -*.,,-..... -... -..:.-~.. '-,."'"":--~"-*' Minimum A~eptance Criteria(> iS.O offspting,per stirViving femaie) c:: 40 c c..... ~ .2 = -*---~-**-***-~*..-*"!'*-,,~._.._...,.. __,;-.,.*----;***-.. d-~*-'*~,--*-"'!'"-.... ';-.. ~.:-**~~;*--~;~~-.*:'i'~~:-~*N*-~**:-*>:* North Carolina.AcceptaneetJmit ( <-40~0%) "Cl 30 et! 0 = Q. ~ ... er:: 20 0 -c = Kentu<;ky Acceptanee Limit ('S SO.fl%) * ~ = .E:i 0 !,;: u 10 ~ i... 0 er= u 50 .. _,, _______., __.~,.. usei>A u;pe;PMso ~o~~d <901b ~~~~t,~~<41~0%),,-,,_,~,~.. -.,.~,,--,- 40 30 10 Control Reproduc~ion, C6effid~Jit ofVariatic>n (CV), or Perc~nt }Minjmuni :a~gnific~nt,D~fference (PMSD) PMSD is-the minimumsignificantdiffer~mce 9.etvre,en the c$;Jptr9] an.d treatmeD,t that can be declared statistically significant. .u. Central Tendency (meaJi ControlReproductiotJ:. CV. or PMSO) Control Limits (mean Control Reproduction, CY, or P.MSD z2.StandarifDe~iations) Page 80 of90 Predsion of Endpoint Measurements Ceriodophnia dubia thronic:Refere*nce Toxitant Data. ~if;.1 En\\ittoomen~t'Testi~s soiiltlonSj Jn<:. Tt?St Te$hfate Cont!'Ol .conti'.Oi M~an q OJ er MSD PMSD CT number suniival Reproduction for Control Mean for Control (%) {offspritjg/fematel .flt:prOdui;tioh (%) Reptodliction (%) f~rPMSD{%) (offspring/female) .PJI%} l. 03"P3..:1s 10() 3Vi s.n l.9 5.8 2 04~()7;;.15' loo 32.3 32.4 4.6* s.. 3 1.8 5.S S.6 3 05-0s-1s* loQ ,31;7 32,1 5.6 5;4 1.& S.8 5,7 4 06-.09-J.5 10cr 29:7 31.S 2,g 4.8 1.4 4.8 5.5 5 Q7~1.tt-1s 100 30;8 31A rn 4A 1.5 4.8 5.3 6 os414~1s' 100 ~l.-0 3,l.3 3.0 4;2 1.6. 5.0 5.3 7 09--)5""15 100 2~.4 31.0 4.0 4.2 1.7 5.9 S.4 8 10*06-15 100 29,6 30.9 4;8 4.2 1.8 . 6.1 5.5 9 i.1--03~1s lOQ 31.7 3LO f;;S 4.5 2.2 6.9 5.6 10 U-08-15 ,100 ~(t6 3\\>.9 4,9 -4.S 1.9 6.3 5.7 11 ch'."12:.15 100 3i2 3o:9 3.3* 4.4 1.4 4.4 5.6 12 02JJ9.-16 100 31.:6 31;0 1.0* 4.6 2.() 6.3 5.6 13 02*P9*1Ei 100 32.0 31.i 5.9 4/7 i.6 5.1 5.6 l4 03~os~16 ioo* 29.4 31_.0 5.4 4$ 1,7 5.9 5.6 15 04-05-16' iQO 29..3. 30;8 6,q: 4.. 9 1.8 6.1 5.7 16 04-05~16 100 32.3 30:9 4.. 9 4.9 1:1 5.2 5.6 17 OS-10-16 10(} 31.6 31.0 5.0 4.9 1.6 5.2 5.6 18 06-07-"16 100 2.,7,9 30.8 2.6 4.7 1.7 6.Z S.6 19 . 07..,1_2::,l,6* 100* 3?..S 30.9 4.9 4.8 1.7 5.1 5.6 20 08'-02'-16' 100 29.9 30.8. 6;6 4.8 2.1 7.0 5.7 Note: tv: *toeffiCient ofvariatiof! ior controirepr0di.lttion;. L~wer CN bound determined by USEPA (10'h percentile}"'8.9~:; UpJ?ef. t\\ibound,deterrnin~'by liSEPA (~ottt percentliel = lli% MS_,<:* MiJUmo'm5ignifu:ant.Iiifferenee PJ1.11SD o::: P'erc¢Jli. Minimum'Si~ifi~rit Diff~reni;e PMsD ls a mea*sure oftii$t {'~lcin, The PMSD is tfie minimum percent difference'between the control and treatment that can be det:fared.statisticallv :Sigi-iificant fii a wtioJ£!eff1uent toxicltv*test;. . lower PMSD bouhd.d¢termined by USEPA(1o1h percen'tlle} = 13%. Uppei"l'MSO bound d¢terrriined bv*t.lSEPA(90m percentile) "'47%. q '= *~entra!Tendancy {~eao to~I ~eprodui:tion, CV, or PMSO) VSEPA; >2QOO. Uilderstari~i~g and Acc01.1nting fot'Metllod Variability in Whole Effluent Toxlt:ity AppJicatio!lS: Under the t,iaticioa!Pollutant Discharge Ellhiiriation Pf0granu EPA-83'3-cR.0()..093, us EnVironmeotal Protectiori'Agencv; Cincinnati, OH. USEPA; 2ooia>::zoulb;.1'lnarRepO'rt: !nt~tfabora~ory VarJab!lity study i>fEPA shorHerin dtroni(:.and AcuteWli61e*EfFhsent Toxicity Test Methtids, Vo1t11nes l and 2*A'Ppendi11: EPA*Bii-a-01,004 arid EPA~8:Zi-.8~oi-oos. Os Envkonmerital Protection Agency; Ondrmati; Oli. Page 81 ot90 l l I _.I J J 0 0 0 1"-.*-~: <):*=~~~~ ~ Sodii.m.Chlotide*Chronic Reference.T.on~nt*Test . !... {EPA-~~AR~o2~013 ~ethod lQ02~9) ~ Spec~: Cedo<i:apluzia d11/iia. Diluti<in preparation nifOTlll(ltitm: .-Comments: NaCl Stock !NSS number:.. mss lC:::.l~ Stock preparation: 100 gNaCJJL:: Dissolve 50 it NaCl m 500 mL MUli-0. water. Oilution oi'eo CimzJL) 600.* 800 1000 1200 1400 stock volume (illL} 9 f2 'lS . :J8. '"2J Diluent'volume (mL). 1491....... 1488 148) l482 1479-Total volume{mL) 1500 1'500* '1$00 1500 15.00. Test orRaiilsm so11rce hi,fi:Jtmtilion: ' 1<!i'til,tformatio1t: Pate and times organisnts \\\\"erc'brim ~&~(n;'!'\\\\o. o~o ~ -~ lticµbawr.nwul!crand'$elf between: loeati9"~ Ctilture board: . i:n,.'\\~r \\t....*A ~epJicarc number:. f, l .I. :t, r 4: s r '6* " 7 I *:s.. r !) l.JO. VWTbati;ll: Culture board cup number:. b.. Q h'.'1.. f'l\\, Jg I "U I "Jli.11.=i l~"l. ~- Transfervesscl infonna!ion:* .. i:>H= &.o~,ti.. Tem!leniture= "'2.$.;~ "C.' ~lilt.as/rum.batch: Average iransfer volume fmL): * : IMl:inL Day 5 08.'-0J'-16 Cotttro/ i11formatiom %.ofMale Adults: % Adults having :;Id Broods:.,; % Mortality: Mean OffSp:ring/Female: %CV: Page82of90 . 'tl!St hiifuitijjn and-fe~dfog, rene\\v,i,tl *n.d feJ:(iing, or* *. termin:ition time J"" 61.. <::40;0% MMSW ,f1atcf1.}fscd .LOEC P~~e1 of.6 l l l l -1 -1 --1 -1 I . J J - j J 0 4 I I .J:. Page2 of6 $pec~gs:. Ceriotlanlttii'ttduhta c-ONTR.O'.L CdNaCICR#: 191 S;utvivat t1itiJ lleJlroductiim Datn. . D.av .. :t *.. 2 . ;3.. .. 4 5. 6 . 7 8 9 10 *1 * .* \\'ouilg produi;e~ CY : *.0. 0 U 0 0 0 0 C) / t'1 Adutti!tlirtalit;r \\,...,.' \\..... ~ L L . L. L L L 2' Vo'qngprod.uuf),. 0 : *. (). ** -~-- h:.* CJ 0. .D. 0 0 .O Adult mor.tafity ... \\.,..... * \\._,., L. L L- _\\;....;. . \\..,,...,.,..._.; . *\\._ l..._ s ... *v.ou11Jpro~~ccd .0 o.. -:""-. f'iif: *.. Ci : D:'. O, .6 0. ~tJtdt'mor;talifr-. L -\\.-. \\....,:.. ~: L; \\..- L..L. 4* Y.oungpro~u~:. q-. ,,_r. ' * ~~.. : 'C\\. -~- 4 * *'""\\ .* '..\\ --~ ':'?\\Uuitm~nf.r .*'L. *y L.. *6. * \\;,;. L \\- L.. L.- \\...... ~. . Yo~ng:erot}~cil. :f{). :, it!:l. ' t\\.. H~ \\ "t,.. .\\,_ i'"t . \\1.. l 0 I L5 Adult mortality L.;... ** ** \\......;.; \\;.....;._, L. L \\...- * (\\...... \\....... C ~- * ¥o~~n~r9thttcq. /:'\\: n f'\\., Q () . M.. tn* . C 0 0 .Muttm~rtar,:.ty, 4* :. L,.;._.. \\.. ~. ' 'i-,. L. * \\.+..... L. L . F'llial:AduJtMonatitV ~ * ~ L...: '--'* '- * \\....... i '\\L... \\..._ L:.c * '-- Xfor,3'C!BrotHJS* '.'~ . ~'. ¥"- * ~ ¥ *.... X... ~-- r... No.t~f. Miil.tmo~ity Q,._<=.:nve. o "*:dClid}; SB'::*".splitbtood(smgfe broO!hplit between t\\Vocday~), CO =caey over (offspong * * -ca~*~ver:Witl'!~tiltdunngirailsfer): * Concentiatihn: ~-~%~M~-~orta~-~H~-~;~'--~~"-""T~~~-.--;1 Meari.Offs rb1g!Female: ~. '\\ R~nlit:i.fe.riuniber .. ru... 1' .2 .J 4, 5 ..6 1 .8 !) IO J Vp11ng produced~ :..,6 l'"'\\. ' 0 c l\\ l'\\. Cl.,. 0 A 0 .... *~iJ~l( l_ll_l)~_t?~..,..... ... ~... k '\\.- L... \\.;,_,. L L, \\.... L-... L .2 Y*c#~ng produced ~o. ' 0 D i... o 0 ... o Q 0 c.. C5 .. Adult mp~lltr L. 'L ).... ~ L. L. L -t.... L '- . J Yo~n~ pt1>d1u:cd* -~... r-... f\\.. << !';'\\ <<}. .. A. (\\ c 0 a A.aw~ nl!li'tal~ L...... L-. \\.._.;:, . L L L .4 L. L L 4 Youllg priid~ctir!. ,q s -~ .. *'4. . '*'{ H: ~ ~ ' 'i .A,~lt mo!'tlint.r..:.. *.. L L. L L \\....., L.. ! L L L. L.. .v:oµng iwoihrced .. l"'l...-..Jo l.\\ Jc;} * : :\\ i;,... .. 11.... fl \\ : 11 l,_ \\\\ Adlitt morfaJify 'L ' L t...- \\;,::... \\..... L ' \\..:-. L.. ~ L 6 ... rouri{'~!'OIIU~li_i!. . f"':l 'h.* . 0 .h .A ("\\ 0 0 0 0 Adult'mortaJit,* \\... L L \\....,.. L L L L L L 1;. 'l{o~g;(J~duciid I Jp tS. '.'S lS' l'"' .tb _,, \\'S l 'S 1~ -TO.t:t1:youngpr<iduceil- -~"(. 1..1:\\. -~' . ~-\\ ~~ 'Q\\ '2:fL ot 3\\ L!( /VD(e,.' J\\dtilt m~lit)i Q.. =]ivC,. D -dead)~SB=splitbrom:l {sing!~. brood split betWcerl two d~); CO= c:arry over (offSpring .~ovet\\vllhadul~dllrigglt.insfet), * '* Co11c(mti'atioll: % Mortaliiv: 01. Mean Offsi:ldng/Female: Page83 of90 S()P ATJ4-ExhibitATi4~1, rc:Vision ll-Oi-14 l I l .1 Species: Ceriodaplinia dubia 800 mg NaCl/L .Survival ali.d Reproditdio.11 J)irtq Day l Youag.produccf . Q, M. () I'. ,C). O * ('\\,. 0 .0', Pl .Adlllt mortality* \\._ (_ i:: L. L.,. :. \\.:_, ' L C . L ' :*L ~- Yo1111g pr.Od~~d :.'.Q... () '(""\\ . * *./""\\* * * "("\\, ['i\\. C\\ :Q*... :C'\\ Ai.lultmortaltiy \\_.. ~- \\...- L L.* ~. \\:;.,,..;. L \\...,. L 3 Y11ungpr.oduccd ~ * * (, ('\\ f"\\ .A* ti '{':\\ ** 6* .O l'\\., Adult m0rtalitY L,. '\\,....:.. *... L. C "\\....... .. L; \\...;... \\..::... \\..... \\;.,.. Young produC:ed '°'. * ~- * *'4 S ' C::::. * ~ --~ * * *q Adilltmorlality L \\..- \\.....,,. * ~- _\\.,,.., \\._ \\..... L..: \\:...,_.. L Voungproihiced \\~ \\L. \\j \\\\ ~ * \\1::1 ' \\0 \\\\ ** * *f\\,. *:G \\\\ Adult mortality. 'l \\ ~ * "- ~- *. **L:-, \\;..,.., \\..,.... * ' \\;;;.,,,,;: :* : \\...-_* * You l.J.; n. o*.. :r*.1 .\\:.. '}.:. C:l.. \\'..J Adultmort.lntY L.:. *c * \\. ( ~- t. .\\. 7 Ymrng prod0cCd: \\ ~ ; l;~. ** * (- *t,p . \\~ ** lL.i. : \\ ~ \\.;g* ' \\ \\..\\ : \\lp. l'\\..\\ * Tota{yo!ing.produced 2 Q. * ~~ ~*"J.;.. "L'\\ '31... l.l. ~'"\\ 21\\ * ~~.. 1:.1;* FinaJAdultwtortality . '\\ \\..:. L... ~-. L L .~ \\;....... \\;;...;_ : : L. Note: Adul.l!,llOitality (l;=liVe,.D":.'deild)~SB=split~oritf{Sill&le'br.Oi:Jdspti~bctw~n\\'oqa~).CO~Cl,llt}'.O~{O.~ carried ovenvillt.adult dUring liiinsfur).. &,ru:e1ttn1ii0.a: ~%Mortali : *. '()'1~. ~ .Meifu Offs o em~e:........;b %:ReductipnftQn:t~tl'ok: : : ~"Z.;$1.. 100() mg N'aO/L Rcnlkntc nilmber ** Day I 2 Yooogpraduc,cd ,(') *.(\\ b Adultmorialiiy L L.:. L 3 Youngproduccd .. h () ("\\* Adult mortall!Y l.. \\,.;;. \\_.. 4 Youngproduccd.* i- ****c.J.. --~- \\.:r **: Adult moriali,ty \\........ \\.:...;..: ~ 5 ll. 1 o.**. J.'.?S i o. '.. 10 :. *... tb. *. u. Adult mortlllify .* L *L * *L; 6 .o ;.h. * *. G... ***_ o,.. 0.. -l1 Young produced ,. t'S.: \\~. I\\: Total young produced .* *-i: '-* i ; "\\:~ FinalAdultMort:llity l~. L.:. ~- * \\..._;..... '-- *. L '*\\;....; *... ~* """- '* \\..: Nore: Adlilt mortality (L ""live., D =dead),. SB,.. splidl;ood (s)n8l1!'broo!l sp!itbetweent\\vo d;i.ysJ.;CQ-=cal'Q"~iirtoff$priQJl, carried*ovenvitb adultditrlng ltlin~fot). * %.tvfortalDy~ 0 l Meafi OftSoriit~Femat~. '.* 4& *. 1 *%Reduction 'lfoin.Cpn~IL : ~-~:07~. Page84of90 SQP ATi4'.,,,,µJiil:iit AT~4.;l;;feYei$.ii)n n-:OJ:t 4 l O" 1 l l l 1 ( -I l . r 1 I l I .) -~ I Page4 of6 Sp~tje~ _Ceriollapkniddtlbia CdNaCJCR #: 191 l~OO'J~~g:~~f;qL;. S'i!>'vtvt;i. n1fiilliProilu,ctiOn bat.a . Repli~~te num:be,r Day' f'f '2 1* 3: . t. 4 J. s I 6 l 1 I. 8. I 9 .I 10 1 - r=v~1i11g:p'ioiuc~(f'; :---o.-* ~~o~ -o cc---o * o... a... a. c o A'1ulhnortiiliiY :.* *\\...;.. ' L L L \\...,.... ~ \\.....;,. \\..--. -~ \\,_. <z r-:y~t.nlii>roii~c¢4 * -h _:. t\\- ~5-o-:- -Fi. ~A-~~ -a Ii o Adfilt1'1t>rtt11ity \\,;..., b 'L.. L \\._..., L . *L.. .*L., \\.-: \\..... -~ * )'qurig.p~duccd* Q. : O.. *O D 0 . :(:) : :!'"'\\,. A 0 0 1 ,'Jid'u~t.modilltty ~:. L...-.. L . L.. * \\,-. " \\.,.;i.. ;.1-. -~-* \\.....,. : \\........ i * *.Y~n!qg.iJrc>dU!!ed:.. ~- ~- * *4. ~-* -~- * -~-.. A* ~ Z "L .~dultm(lmlity L, ~- \\......_..,\\.....;. '..J........ L i*~. \\..... 'L. \\-.. s*~*l~~::::~r~t:J: *t.l*2--Jt.?**r-.tJ *~*-t~ * i~~: c 1 c. . :l"cmngpr.oauia!d' .:D A * *o o ,r:;.. f\\,, : *a o. -o *.f"I .* ~uUJ11or1ttli.i¥ . \\._;.. e:** *L,.. \\.....J G L; '- \\..... L 7 * -y~u~:prp!lueiid. ..:5 * "".L q. '°'.'\\ ~ : * \\o. L.\\ u. ">~,_ _ t:tit:ilfPu~priirJu~ 1. t~\\ J ~ 1,.,~ l i4J.,4 l t~ : I t o:f i I -~ I. 'i lFiriiil:Adult'Mliriallii~- I Lk ~! L-I Lt -.g:**~l:3fI'C I *c.*1 C . N014:r Aduft'm!>ftality, :(L-= live, n= ~l. SB'.= split fiioo4 (smglebroi)d:splitbel.\\Vc:en.twl5d~;CO=-cariy t)ver(offspi'ing Cltrried' over1"!"th.~i.iu!t 4urtng"'91Sfer}, <<:onf.iimtfittiQrt:, % Mortaiicy;. Mehri Offspring/f'emate: :~ lt~d.ui::Pon from Control: 140.Q.m_gNa;Ol!;,.. *. .$ziruJ1ml"(lnil RejJr(t~ilt:iion /;)Ma Rcnlii:nfe number J>ay l L 1 J.. 3 I 4 J.5 I - 6 I. 1 J 8 1 Youmi pri>d~d~ ~if':-j * **_.-,..,...*.**I . 'A;.I: J':> I ""' I *A. I* ~. I,.... -~9. t I .L,J .. ~., .LJ, U_ . t J,. L..> .... l/.:.J. f;_J "+dult~oft;t~HY,: J L,,.I * 'l:.- 1 ~-' J, L. F \\..;,.. J L LL IL a' Wimgpr9ducet1 1* !"*:1 -~ -1**********:r..*-:1** -Q .. ':1~0-*f* O********..,........ **Yo'\\ **1: Q* ~-* r.. u ..... u....,_ -....,...... *"***...... ~. --~- .. -~~fo~rt?.J~ J:~r~,~-r \\..,...-14 l: L J. L,.,.J ::c.1 '- ~ Voll~ jirod~~:. 0 ... ~tlult,mortai<<)'... ~ L. 4 * : _ youngprodliccd 0 .. * ~duTtuiortilli6'.* . L. L. L "S I Y~iingptptl~... *... *"'.L I . O.. ** * ~-- ~- .. 'L... 1 Actu1tm9~tilnry,. *. L. L \\,,..,. * ""- ... 4 L .u 'Vo#l1g{J~dlic!lii)~D.r *o: I. 'f) I 0 .~.Cl J *.. *~.- I (). I :n Aaut(niorbilitYj --c::r-t..... J 'L -i ~--r~\\;......J ~LI,. L l'L. '7 1. y-0u~pi;otJncflft l.:.:1-..1. ti :1. \\... I. 2>.. L 1... I 6 I ~I - D Tqtrilf.!Jt1~Prd~D-Ccd I 14 J l. J 1-l -~ I ~- I. '-t I \\.\\. I ' 01. n.. \\ Co'l.'\\£. . 9 .('J L 0 \\..... _Q_ \\.._. 2 L 0 \\.... n ~ .t""i "1... lC). r-. L.. -(5 L. .Q._ \\.....,. .!L L '-( \\.... .o l._ 0 1{ I Fi11aJ.A<JoJtMortali!Y'_c~ ---'--~L C::l \\...... " r.'L,..;_ :I.'\\o.;,.. J. L. 'I '"L..I'\\;- I.L "]-\\..... \\. ,Nole: AiJjjlt:ri)~i1*l(ty, :cr.*=-li~~E>= tfe!id); sa..:~plit l!_ttiod {single l>fi:\\ad.'spli,t betwet:JJ'twodays); C0"" ~flYO\\ier (OfJSpriilg ~me(lov!?h\\litll*a~lt:~,~),, * Coiicenlratl<>:'#:. '%Mo~litv: Mean Offsptingj'Female: % ReduytiQn frontControl: . Page 85 of 90 .SOP A7~4-...*EJdiibitATl4~l~ revis'ion 11-0 l-14 ~ ~ 'g Co C> () co --~. ..~~ .) ) Verificatfon.of Ceriod"plt11i<t Reproduction Totals Control lODO mg* NaCl/L 0 Replicate number. 'ft>liil II)' I 2. J. 4 5 6 7 8 9 10 Dily l 2 J 1 , 0 0 O. 0 0 *, ~O 0 0 * *O .0 .0 .. r... ' 0. 0 ' 0 2 o. O* 0:.' 0 " 0 . 0. O.. 0 () - .0 0 - 1- O 0.. 0 J. ..o _ o* .o : o o o _ *o o o o. _ o..... ,3 o- 0. - --* 0 4- " 4 4 _3 -,J s - *3 ; 4,. 4 4 ' 3: ' 17 4. . 4 '_J - i4 5 --* **-* IO... lL _ 10: 12. J2 9.. 12. IO:'.... 10 - l<l6.. ... s:*..... 12. IO,-. rn .G. - .0.. JL' O* . 0 0 0. .0. 0 0 ! 0 -. o -:Ii* '** 0 -.o.,- _(I 1 . J3 - J7.. rn.. M. 16 1.7 17 U 1.S.. l6 156 7 'IS.. l5- .. 14" -.1*otiil 1.7. Jl. 30 27., 33* J:i 30 .3J 2:9.i,,29*- 299 - :rotnr. .Jl .28 28 -600; mg NaCJlL ,120Q mg NaOJ/L 011y n.cntlcntc*llunlber* 1'ot,111. l - .2 .-.J-i $. G -'7* 8 ' !> -* ljl.' ; D~y I. '2** 3. . 1 0 o**. 0 o o. o: o.. 0 : **o** o: 'ff t <O. o*.. o*. 2 -.o 0 O - *. _ 0 ... 0 0 o_ 0 o ir O .:2. 0 J) ,.0 J .Q*' .Q (f - () . *O..O* () :. 0.....*. o*.**_ *_Jt... *O... J Q, 0 O* 4 4 .. 4' ' '5, - '3 4 : ii. 4 5. .3:... 3.. - :19 'jf 3 ' *3 c2.' 5 12. H> *. u. ; 1.0 ' - 12. 12.. - ll .II.. 13 ll JlS 5* 6.. 4*.** 7 6 0 o* *o:..., JL:.. 0 0 0 o.. v o::.- U. - ii. - -o-0. : O' 7; Ip 15 15 ' 18., 11.*: 15 T'7 - lS : lS 'ts' __ JSS .5. i 4 Total * *... 3%: 29:'.*,.3:1. :.31 J3 Jt -32" 31 *.. n. *29* 310 1fohil 14 i 9* 1.3.' . 8P.Q*~gNaCilL 1400 mgNaCllL 1tttilicnti.i'iii1mlit!r ~------.- 4> - *S': * - 6; _'7:~.. : th '9 -; '10>> Toti1i -Dn)r 1 2 - 3, Uily lJ 21*3 - .I. .O.l 0 l 0 .tJ" ' '{) o. '*{), 1 0: 0: tF - '*.Q "... 'r o* O' - :o- - l l o Tc;a-~r.o * -0 : __ :ir*, ' 0:. () '(.! :; - (I -- (),. ... ::lL... 2 ff". *:o... -:o. .****'J. o.J 0 I O', O< ' ll.-. (f. 0. - 0. ' 0. - O.: ,() .l. o.. O ,o**: ---*-****4 rrs:].4: 4 '3. - :3 . 5- ,,A*: -4, 4, .. 39"' 4... - _- *. ::1L* '(l, '_] s - 12 J J2J J2 .n.,J3* lO JI 11 - *13* ' JI-1'1'.6. .:5* 2 l' 0,.. --- i'i .o l'it I o _ O* ~.,. O'... o* '() o i) *o ..,.:o ti... 0.- - () 0. ... 1 15 *t l:l J 16 J4. *'16. IS< 'JS 14 Hi.* IA: JS! ~1 ~* . Q;...... 'tutnr*;.rlif r 31lJ.az :* ;2l)'. J2'.28:, -34, Z9' 33' ',2l>>: ' 3_06 ..iro1111. .4 --...1.. :..:.L ncolit'ntc number* Ti1tal .4 5 6 7 8 9 lO .O* 0 0. O* _* o- .0 - O' 0. - 0. 0 - 0, -, o.... .. 0 JJ 0 O 0 0 O;. 0 0 0 0 0 5 ,4_ -..,:,i* A*_ 3_ J. 4-38 . I) Hr* *. [3 - JO*.. -~10 IO,, - 107 0 ' o_... Qi; o* O* - 0 I} . J): 16 J3, Iii l:l 13 .16 l.3 142 *--* - 32 21 '3.l" 'f).7 26 ' :2!J 28. " 281- _ H.imlii:nte tll1mbilr*' T9r1,1.i 4 ' -5 6 8. 9*; (@;* - -o - 0. 0 O* - o*. --.0 ' 0 0 0 .0..o. 0 - 0. O' - 0 0 ' 0 ' o* .i) o. .. l)* lL 0 0. .- J. 3* 3 l* '.2',.2;.l -.. 24.... 3 ' 6 .6: .. "5.'.. z; 2 -, 5 46 -,0. ..0.' '.(f. o* -0 :, l} ' -:o* () "l S. 6'. 4 :* 4* 1'. '2 4 13* 14' *is. lfl. II (i -9 lU ite1111cl\\ic. mil'libcr **- 4 s ' 6' .,,:**. lf. -.9 Tpl11l rn* 0 0 o:' -*ll'.:. '0: -* - _Q* ... O.* 0 ' ~o.. *o. o: 0:.* - 0 l)_ 0.*.. -- () _o*.0.. *O.. .O*. !l - :'O:.: Q 0. 2.-. 0 ' L.. ]t j),: '2 0 JC _Q .. 'J. 0 2. -:r () 4 J3 IY * :, '!} __ :y ()' i 0* - :o* *-o t 3 ;. ':2:* o-** 2. 'Jl :' ()* o tq** 5 ~*. _ 4-T 2 4 Jf' /: '!'... ~ ~ar ) ~n'vlronm~ntal testing.Soiutlpns, Inc. Ciiliccmracio.11 (mg/I, NnCI) ? 3. Coufrol 27 31 30 .600. 32 29 3l soil 30 3'0 n HiOO 31 28* 28 1200 14 9 iJ 14110 4 2 l>11i111c1t1s'MSO value: 2.JOl }'MSQ:.. ~ M Ceriodapl1!tia tit1bH1 Obr9nic P,eferen~e l'oxical)t T~st ltPA-s21,,,R.. 02~ou, Metliil~looi;o Q.1uilicy Control V~rification of l)at? Entry, Caf~ulation$, and Statistical Anlllyses ,f 27 31 29 32 i3 . s MSD.. .PMSD=. Test 1111mbcl'I . CdN11CICR: #191 Tlist dnfos: A11g11st 02*1!9, '2(116. lh:nlie11tc Jii1mber Sur\\'.iv~l.Avcrug_e*rejwqdlii;tbiir* C<ieflii:ient uf l'C:rciml ~~dimfon lton1 s (;- 7 8 9. to (II)~) tol'fSpri11glfc11la fo) vl1tl~llon ('(.)' c0fl1rol (%)' 33* .32-30 31 19 29 100 2M* 6.li Not*appiic11illc 33 3.l 32 3l 31 2\\) lQO .Ji.I)' 4.U .J.7 32 28 34 29 33 29 ioo 30.6 6*6 -:u 27 3i 27 26 29 28 100 23;7 7,t) 4,ii 14 15 ro s 6 9 IOO IU 27.7 ~2.? 5. 4 '4 2 4 IOO )> 48.4 89~3 Mittiini1m Sig11ilic11111 Di£lcrente . Pcri:c111Mlriim11m Sig11i!Jcm11.Qiff(lrcncc l;MSD is n n'iclistm: of test precision.. Ti1e PMSO is. the 11\\inimum perCl!nt di!Tcrencc bclwccn the control. 11nd treauncnt thot. cnn bc*dcclari:d sinlisticnlly significnill inn wlioie cffiuent Loxicity*tt:St. towefpMSDbound dctt:rmincd by USEPA (10° 1 perccndlc}"'13%. Upper PMSD bound determined by USEPA (901h percent!ll::)= 47%. Lower and upper PMSD bou.nds we~ dctcmlincd from the 10th and 90th p~rccntllc, n:spi:cti.vely~ of PMSD dnta from EPA's WET lnterlaborntory Variability Study (USEPA, 2.00 I a; US EPA, 2001 b); USEPA. 200 In. 200Jb. Finni Report: lnterlaborntoiy Vnriohility Study of EPA ShOrt*tcrm Chronic 11nd Acute Whole Effiucnt Toxicity Tes.1 Mcihods, Volumes I* untl 2-Appcndix; EPA-82 l*B*Ol *004 nnd EPA-821-B*O I *005, US 1!11vironmcn111l Protection Agency, Cinoinnu1i, OH. Stan Date: s12t.201s End Datil': a19j201 s Sample Dale: Comments: conc-mg/L O"Corit~l 600 800 1000 1200 1400 27.000* 32.000 30;000 .31,000 l4.000 4.-000 2 31.000 2e-.ooo ao:ooo 28.000 9,000 1.. QOO Statistical Analyses ceriodaphnla**suritlVal*and Reproducti.on* Test-i;teproduction.. Tesno: *CdNaC!CR . Sample.ID: REF.;RefTox\\canl' .LablO; ETS~EnVir. leslin9.Sol,. Sample Typei ~l-S~ium c;ilio.rl.de Protoeol: F\\iifCl;!R-!;PA-8?1-R,02-01 s Test Species: c;:i;i~cerjadaplloi<> dubia 3 3Q,OOO 31.000. 32.000 28,000 13.000 +oao 21.000 31.000 29;000 32.000 13:000 !;:oop 33.IJOO. 33,000 32,ooo v.oo.o '14.-000 ~.0.00 32.j)OO 31.000 28.000 31,000 1{;\\000 4.Qoo 7 sp,ooo 32,00ll 34;000' 27.000* 10,000 i;;OOil' 8 31.0.00 31.00P ,zs;ooo 2s.ooo a~oo.n 1.000 9 2!:1;00.0* 31;000 ss~ooo is;ooo ti;coo 2,000 ~gd ~.000 28.000 . 9.0.00 4.QOO. Transform: Untransf'arined* 1'-Tailed ISOtimie Coni:-mg/L Mean* N;Meail Mean Min, Max. CV% N t~Stat Crit<<:a:1 Msc Mean * *..N~Mean* D-Control 29:900 1,0000* 29.900,;mooo .33,000* 600 31.000 1J~368 ~1.000 2s:oo.o 33.0.0(l' 800 30.600 1,0234 30,600 2~0!)0. 34~.ooo 1000 2a:wo 0,9599 28.700 2s.ooo ~.(loo 1200 11.iOO 0,3112: '.11.100 6;000 1s;ooo 1400 3.200 0.1070 '.32110. 1~000 s;qaa* -. Auxillary Tests l<oJmogorov D Tesl'indic;ltEis norl'llal dlstrjblltion: (p.> 0,01) Bartlett's Test indicates eoual.vaciMces {p,,,.0.15)' Hypothesis Test ('Mall, 0.05) NO~.... 'LOEC CliV Donnell's Tes! 1000 1200 1D1:J5.45 Treat1'11e11tsNs D'COlltrol s.;Sas Hl -04,023 1'0 -1.197 6.572 10 ~i);762 ti,978 10 1;300: 21.s10 10 20:41>3 48.412 10 29;(!62. statiStlC 0~926.4 a;o1111, TU. MSDu* .MSOp. 2:10oe4: *0;!>7926 Linear Jnterf)o!atiQil*@lf! Resal'!lP!eS), Point .mgfL 969.444 1014.2 so.... 95% 'CL.. ..Skew .30.500 2,2a1 2.101 ,~o.sqo. 22a7 2:H>1 30.500. 2.287 ,z;jj)1 :2a:'700 2.2a7 2'101 i~cicio 2,287 2~101 ~-- a.200 .Cl'itit:al Skew* 't.Q35* Q;064G 1s:ci$sa .MSB. .. MSE F..Prob* 1466;94: 4,~Q3;<* '9,3E,40 '!COS IC10 IC15 IC20 IC25 IC40 IC50 . 1031.53 1048.8'5 1013!3.19 1.11.S.18 1152.84 46.6e<>S '857;991. 11)10;6.,0.6536 8.37526 9~!Ul45 1027.~.!!.~544 s,57571 101e.s9 1o#.2s: o.osso .f.0~-'-'-----.....,.--,-,,.-, S.1'725 1036,55 1060.. 77 0.1788 s;s422s 1os<1.1s 1ons1 0:2398 6,40685 1104.94 11?1.,2 0.2308 7.5251 t13'7.93 1169 0:1919 Q.1l o.e* o.7 ... o,s: g o.s i oA a; o.-3 0.2 Ct! o.o,.6........,... . _,,,. _______.o.-"--<6' -0;( >jo..,,_,,-.-_,_ __._......,.,_.......,........ --1 nose-Response Plot 40...----_,,.,.----,------.,.------..,., 301.-~~--t--~-._,...l/->-.~- ,g 25 ,s:20 e* c. ./}. 15 10 Page 88 of90 500 t~O ooserogfL 1.ftiill; o.os tevet of:Si~cafirie. 1,500. '1J>OOO {oaoo ,,,001)0 O;S4,1Q Q.3639 ~J.1049 Kiln -CJ..S:499 df. J?,Sf l l l l l l l _I I I g r: ~~d2 Concentratfon CONTROL 600 nig NaCl/L $00.mg ~clJL .1000.mgNaCtlL x200 mgJ'faCllL Analyst Nranieter pH(S.U.) I>Otmg!L) ColJ()uctiVity µmhos/cm) '*AlkalinitY ( CaC0;1L) Hardness ~m" CaC03'L) Conducllvity { mhos/cm) . :"Temperature, (°C} pH(S.U.} ' GoniluctMty ( hosicm} ... ~emperatur~ ("C) pH(S,U.) Conductivity fttmhos/cml .. *Temperature ("c) pH ($.U.) necmg(L) 1400 mg Na.CUL. ConductiVity << os/cm} 'femperature.("C).. Page 5 of6 CdNaCICR #: 191 '*1'¢m~Ul~ Jl'.llrf;oi'riled at the time ofti:St. foiiiatipn, renewal. nr lerininiitfo1t by lhc analyst i_deiitiried m the Daily Renewal InrOiJJUl. ti~n ;rabklocat..cd on Pa~ L Alk.*alinity anritess perfom1ed by the anrifysl identified on 1be beitch sheet specific for each analysis and in'Jnsctillcd:to this !Jencltsheet:by: Page 89 of90 SOPATl4--EXhibitATl4.1, revision i 1-01-14 -i l -1 "PT~ ~ ;fik:J r:.~;:.. ) W.~l:.~:*;__ Species; Ceriodap/mia dubiii CdNaClC!t#: 191 1------------------------------------- 1 .Analyst 1-----------...------------ Concentration P a ram et er l pH{~.U.J DO(ll'ig/L) l CONTROL Conductivity ( mhos/cm) Albli.iJity {mo CaC03/L) '"Hardn~ 1 (m° CaCO.i/L) {Anal !St.identified foreiich da l Temperature ._ __________ _...f_C~) __....., __ _.,,.._.......,....... ---~.._..__......... _.~.........,_......,_. __.._11----.._..--........._o!i--~.....,_.,, 600 mg NaCJ/L 800 mgNaCUL pH(S.U.) DO {aiglL) Conductivity mhoslcm). DO (mg/L} 7.g Conductivity }830 C hos/cm) Temperature (°C) 1 pH (S,U.} 7.fO no (mg/L) . '7."l 1000 mg NaCJ/L Conduc~ivity . "'I "'I..J* *.{) { mhoslcm) ""et l ~~mperature "1>\\.<o Jo------+:.pff~.-(s-;u-.J--+7!!:1!'.... i~f:>--u-~~~1-~....,.-u-~~~~~~+~~~1---""'lt-!----1 .I DO (mg/L) 9~ 0. 1400 mg NaCllL Coqdoclivity S>. 6 i 6 ( mhos/cm) ~~.e 'l,~.. lq ~1..\\.1 Initial Final 'Firial Initial ii'inai hdtiaJ Temperatures perfonned at.th~ tirm::0f 1t:st* initiation, renewal ortenninatiQo by the aMI;iSlide!ltiliC,!:in the Daily'. Ren~ lnfo~ation lab!e lo::ated on ~age.I, Al. klil. i~ity antrdn'e$spedonned b:Y lite analystiilelitified on*ihe.bencb~heet~.fic foreacb anal}'SIS.and transcnhi:d to tins heilch sheet by: Page 90 of90 SOPATl4-E;,chibitAT14.l,t~visionJ1*0l~i4 1.'1-"1 Final}}

ML16287A416

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