ML11187A013

2011/07/05 Indian Point Lr Hearing - FW: Zebra Mussel Reports for 2003-2010

ML11187A013
Person / Time
Site:	Indian Point
Issue date:	07/05/2011
From:	- No Known Affiliation
To:	Division of License Renewal
References
Download: ML11187A013 (60)

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1 IPRenewal NPEmails From: Gray, Dara F [DGray@entergy.com]

Sent: Tuesday, July 05, 2011 6:19 PM To: Julie.Crocker@Noaa.Gov; Stuyvenberg, Andrew; Imboden, Andy; Turk, Sherwin Cc: Dacimo, Fred R.; Sutton, Kathryn M.; ezo li@goodwinprocter.com; Dowell, Kelli; Mark Mattson; Caputo, Charles; Curry, John J

Subject:

FW: Zebra Mussel Reports for 2003-2010 Attachments:

2010 IP zebra rept.pdf; 2003 IP zebra rept.

pdf; 2004 IP zebra rept.pdf; 2005 IP zebra rept.pdf; 2006 IP zebra rept.pdf; 2007 IP zebr a rept.PDF; 2008 IP zebra rept.pdf; 2009 IP zebra rept.PDFHi Julie and Drew, Here are the monitoring reports from our "zebra mussel monitoring program" as promised. If you would like a copy of the SOP, please let me know. We hope to have the remainder of the thermal information to you tomorrow. (although it will likely be piece-meal given our email restrictions). Please let me know if you have any questions. Thanks From: Mark Mattson [1]

Sent: Tuesday, June 28, 2011 7:17 PM To: Gray, Dara F Cc: Zoli, Elise N

Subject:

Zebra Mussel Reports for 2003-2010 PRIVILEGED & CONFIDENTIAL, ATTORNEY-CLIENT COMMUNICATION, ATTORNEY WORK PRODUCT, PREPARED IN CONNECTION WITH/ANTICIPATION OF LITIGATION Dara-attachedaretheeightmostrecentannualzebramusselmonitoringreportsforIPEC(2003through2010).EachannualreportpresentsthecumulativesummaryofmonthlymonitoringattheIPECunit3intakeandthebioboxesinplantatUnit2andUnit3ofthetotalbiomassoftheentirebiofoulingcommunity(wetweight),andnumberofeachtypeofmusselspeciesfoundonthesettlingplatesduringeachincubationperiod.LetmeknowifyoualsothinkweshouldincludetheprojectSOP,andI'llhavethatmadeintoaPDFandsenditalong.Takecare.Mark><((((:>><((((:> ><((((:>><((((:>MarkT.Mattson,Ph.D.,VicePresidentNormandeauAssociates,Inc.30InternationalDrive,Suite6,PortsmouthNH03801DirectPhone:603.319.5307Cell:603.345.0071 Please consider the environment before printing this e-mail.

Hearing Identifier: IndianPointUnits2and3NonPublic_EX Email Number: 2725 Mail Envelope Properties (DCB622189B67AD49AE39CD3ED1B4D99D0C196EE4)

Subject:

FW: Zebra Mussel Reports for 2003-2010 Sent Date: 7/5/2011 6:19:26 PM Received Date: 7/5/2011 6:21:00 PM From: Gray, Dara F Created By: DGray@entergy.com Recipients: "Dacimo, Fred R." <FDacimo@entergy.com>

Tracking Status: None "Sutton, Kathryn M." <ksutton@morganlewis.com> Tracking Status: None "ezoli@goodwinprocter.com" <ezoli@goodwinprocter.com> Tracking Status: None "Dowell, Kelli" <kdowell@entergy.com> Tracking Status: None "Mark Mattson" <mmattson@normandeau.com>

Tracking Status: None "Caputo, Charles" <ccapu90@entergy.com> Tracking Status: None "Curry, John J" <jcurry2@entergy.com> Tracking Status: None "Julie.Crocker@Noaa.Gov" <Julie.Crocker@Noaa.Gov> Tracking Status: None "Stuyvenberg, Andrew" <Andrew.Stuyvenberg@nrc.gov> Tracking Status: None "Imboden, Andy" <Andy.Imboden@nrc.gov> Tracking Status: None "Turk, Sherwin" <Sherwin.Turk@nrc.gov> Tracking Status: None

Post Office: IPCEXETSP001.etrsouth.corp.entergy.com Files Size Date & Time MESSAGE 1611 7/5/2011 6:21:00 PM 2010 IP zebra rept.pdf 179463 2003 IP zebra rept.pdf 138939 2004 IP zebra rept.pdf 190574 2005 IP zebra rept.pdf 173133 2006 IP zebra rept.pdf 174742 2007 IP zebra rept.PDF 199105 2008 IP zebra rept.pdf 284776 2009 IP zebra rept.PDF 159438

Options Priority: Standard Return Notification: Yes Reply Requested: Yes Sensitivity: Normal Expiration Date: Recipients Received:

Ref. No. 20407.005 3 February 2011 (sent by e-mail)

Ms. Dara Gray Indian Point Energy Center 450 Broadway, Suite 1 Buchanan, New York 10511

Dear Ms. Gray,

This report presents the November and December 2010 sampling results for the Indian Point Zebra Mussel Monitoring Program, conducted by Normandeau Associates for Entergy (contract 10150496). Settlement plate sampling was conducted on 2 December and 21 December 2010 at Sites 1 and 2 (Unit 3 intake) and Site 3 (one of two bioboxes at Unit 3). The collections from 2 December represent the November sampling period, which had been postponed from late November. The biobox at Site 4 (Unit 3) was sampled on 21 December and the biobox at Site 5 (Unit 2) was sampled on 2 December. No biobox samples from Site 6 (Unit 2) were collected on either date. Cumulative results for calendar year 2010 are summarized in Table 1 (Unit 3 intake plates), Table 2 (Unit 3 intake water quality), and Table 3 (Unit 3 and Unit 2 biobox plates). Low numbers of zebra mussels (Dreissena polymorpha

), dark false mussels (Mytilopsis leucophaeata), or both, were observed on six of the 15 settlement plates collected on these two sampling dates from the Unit 3 intake after exposure durations ranging from one to six months (Table 1). Most of the observed mussels were on plates that had been exposed for six months. All mussels on the plates were small (0.7 to 2.9 mm), reflecting both the relatively recent settlement (<6 months ago) and slow growth during the colder months. No settled mussels were found on any of the settlement plates that had been deployed for one month in the Unit 2 or Unit 3 bioboxes. If you have any questions, please contact me by e-mail at plindsay@normandeau.com or by phone at 603-637-1149.

Sincerely, NORMANDEAU ASSOCIATES I NC. B. Paul Lindsay Program Manager

Cc: Kelli Dowell Mark Mattson Table 1. Density, length, and biomass of bivalves on settlement plates collected at the Indian Point Unit 3 intake catwalk for the Zebra Mussel Monitoring Program during 2010. Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 22Jun10 14May09 13 a 1 c 1 c 1 c 2 821.2 200 ZM 200 0.7 DFM 200 0.6 2 1360.8 200 ZM 1000 0.8 0.4 DFM 400 0.7 0.3 2 278.4 200 ZM 400 0.8 0.0 22Apr09 14 a 1 559.0 200 None 1 c 1 c 1 c 2 1631.4 200 ZM 200 0.5 DFM 200 0.7 2 265.0 200 ZM 600 0.7 0.1 DFM 200 0.6 22Mar09 15 a 2 1082.6 200 ZM 200 0.6 2 1490.8 200 None 18Dec08 18 a 1 c 1 c 2 d 371.0 200 ZM 600 0.8 0.2 DFM 200 0.8 2 d 627.2 200 ZM 1600 0.7 0.2 DFM 800 0.6 0.1 16Oct08 20 a 1 c 17Jun08 24 a 2 d 855.2 200 DFM 400 0.5 0.2 16Jul10 22Jun10 1 1 346.8 200 None 2 223.6 200 ZM 400 0.7 0.1 DFM 600 0.2 0.1 22Jun10 b 1 b 1 416.4 200 ZM 200 0.4 DFM 200 0.2 2 339.4 200 None 22Jun10 b 1 b 1 405.0 200 DFM 200 0.2 2 319.6 200 ZM 800 0.4 0.1 DFM 1800 0.3 0.2 20Aug10 16Jul10 1 1 1066.4 200 ZM 400 1.2 1.1 DFM 600 1.0 0.8 2 505.6 200 ZM 400 0.4 0.2 22Jun10 2 1 1192.6 200 ZM 1800 0.9 0.8 DFM 1000 0.5 0.6 2 1462.2 200 ZM 2400 0.5 0.3 DFM 1600 0.4 0.2 22Jun10 b 2 b 1 898.2 200 ZM 1600 0.4 0.1 DFM 1600 0.3 0.2 2 1136.0 200 ZM 600 0.7 0.5 DFM 400 0.5 0.2 (continued)

Table 1 (continued) Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 22Sep10 20Aug10 1 1 273.0 200 DFM 200 2.8 2 138.2 200 None 16Jul10 2 1 1623.4 200 DFM 200 0.7 2 1252.4 200 ZM 200 0.4 DFM 1600 0.5 0.2 22Jun10 3 1 1960.8 200 ZM 1400 0.8 0.4 DFM 2600 0.6 0.2 2 2295.6 200 ZM 200 2.1 DFM 1200 0.5 0.3 22Jun10 b 3 b 1 1868.8 200 ZM 800 0.4 0.1 DFM 600 0.6 0.1 2 1455.4 200 ZM 200 1.4 DFM 400 1.0 0.5 20Oct10 22Sep10 1 1 14.2 20 DFM 20 0.6 2 18.6 20 None 20Aug10 2 1 685.2 200 ZM 600 0.6 0.6 DFM 400 0.6 0.1 2 1026.0 200 ZM 200 0.6 16Jul10 3 1 2212.0 200 ZM 600 0.5 0.2 DFM 200 0.2 2 2063.2 200 ZM 1000 1.2 0.4 2Dec10 20Oct10 1 1 4.8 20 DFM 20 1.9 2 6.0 20 None 22Sep10 2 1 22.2 20 None 2 26.6 20 None 20Aug10 3 1 c 2 2596.0 200 ZM 1400 1.7 0.7 DFM 200 1.0 21Dec10 2Dec10 1 1 5.0 20 None 2 5.0 20 None 20Oct10 2 1 4.4 20 None 2 10.0 20 None 22Sep10 3 1 24.0 20 None 2 13.8 20 None 22Jun10 6 1 3940.4 200 ZM 400 1.4 0.1 DFM 800 0.9 0.6 2 1495.2 200 ZM 200 0.8 DFM 600 0.8 0.2 22Jun10 b 6 b 1 591.8 200 ZM 200 0.9 DFM 400 0.9 0.4 2 4828.0 200 ZM 400 1.3 0.8 DFM 600 0.8 0.5 Footnotes for Table 1: Site codes: 1 = center of intake catwalk, in strong intake current 2 = north side of intake catwalk, in low-flow area Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Collection delayed because Site 1 and Site 2 were inaccessible during June 2009-May 2010 due to a problem deploying stairs to the catwalk used for sampling (exposure duration for these plates was longer than originally scheduled) b Deployment delayed because Site 1 and Site 2 were inaccessible during June 2009-May 2010 due to a problem deploying stairs to the catwalk used for sampling (exposure duration for these plates was shorter than originally scheduled) c Not collected (the settlement plate was missing) d Plate had been out of water 11-26 February before it could be transferred to a new rack replacing the old one, which was badly damaged by ice (any settled mussels present on 11 February could have been desiccated)

Table 2. Water quality data collected monthly at the Indian Point Unit 3 intake for the Zebra Mussel Monitoring Program during 2010.

Sampling Date Surface Temp. (C) 4-m Temp. (C) Conductivity (mho) Salinity (ppt) Dissolved Oxygen (mg/l) pH Turbidity (NTU) 22Jun10 24.8 24.8 4150 2.4 6.6 7.4 12.2 16Jul10 28.5 28.5 11000 5.9 6.8 7.4 6.8 26Aug10 27.5 27.5 7000 3.8 5.6 7.4 11.5 22Sep10 24.1 23.8 9990 5.9 7.2 7.1 27.0 20Oct10 13.4 13.1 2440 1.8 7.3 7.4 10.1 2Dec10 7.6 7.6 429 0.4 9.0 7.4 22.5 21Dec10 3.5 3.5 2650 2.5 5.0 7.4 12.2 Sampling was conducted at 4 meters depth for all parameters and at the surface for temperature only. No sampling at the Unit 3 intake was conducted during January-May 2010 due to lack of access to the sampling site.

Table 3. Density, length, and biomass of bivalves on settlement plates in bioboxes sampling chlorinated service water at Indian Point for the Zebra Mussel Monitoring Program during 2010.

Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 22Jun10 May10 1 3 4 5 a 5 a 6 b 0.8 0.6 1.4 1.6 20 20 20 20 None None None None 16Jul10 22Jun10 1 3 4 5 a 5 a 6 b 4.4 2.8 3.8 2.4 20 20 20 20 None None None None 26Aug10 16Jul10 1 3 4 5 b 6 b 4.8 1.8 20 20 None None 22Sep10 26Aug10 1 3 4 5 b 6 b 12.8 10.6 20 20 None None 20Oct10 22Sep10 1 3 4 c 5 6 b 3.0 8.4 20 20 None None 2Dec10 20Oct10 1 3 4 b 5 6 b 0.8 1.0 20 20 None None 21Dec10 2Dec10 1 3 4 5 b 6 b 1.8 6.2 20 20 None None Footnotes for Table 3: Site codes: 3 = Unit 3 chemistry room 4 = Unit 3 central control air conditioning room 5 = Unit 2 Monitor #1 6 = Unit 2 Monitor #2 Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Two samples were collected from Site 5, instead of the one scheduled.

b Sample scheduled for collection by Entergy personnel was not collected.

c Settlement plate was dry and could not be processed (no water in the biobox).

Ref. Nos. 19713.000 18 December 2003

Ms. Dara Gray Entergy Nuclear Northeast Indian Point 3 NPP P.O. Box 306 Buchanan, New York 10501

Dear Ms. Gray,

This is the December report for the 2003 Indian Point Zebra Mussel Monitoring Program, conducted by Normandeau Associates for Entergy (contract 4500522928). Monthly settlement plate sampling was conducted on 9 December 2003 at Unit 3 and on 10 December at Unit 2. Cumulative results for calendar year 2003 are summarized in Table 1 (Unit 3 intake plates), Table 2 (Unit 3 intake water quality), and Table 3 (Unit 3 and Unit 2 biobox plates). At the Unit 3 intake, no settled bivalves were observed at sites 1 and 2 for the one-month or two-month exposure periods. A single zebra mussel (Dreissena polymorpha) was observed on the three-month plate at site 2. Both zebra mussels and dark false mussels (Mytilopsis leucophaeata) were present on six-month and twelve-month plates. The highest density for both species combined (3,000 mussels per square meter) was at site 1, in the main intake flow in front of Unit 3, on the six-month plate. Lengths ranged from 0.7 to 1.8 mm for zebra mussels and 0.5 to 3.2 mm for dark false mussels. The small sizes indicated that all specimens were fairly recently settled. The six-month and twelve-month settling plates had a heavy growth of barnacles and hydroids on them. No bivalves were observed on the exposure plates from bioboxes sampling the Unit 3 chlorinated service water at sites 3 and 4 at Unit 3 or at sites 5 and 6 at Unit 2 (Table 3). If you have any questions, please call me at 603-472-5191.

Sincerely, NORMANDEAU ASSOCIATES I NC. B. Paul Lindsay Program Manager enclosures

Cc: Vic Nutter Mark Mattson Table 1. Density, length, and biomass of bivalves on settlement plates collected at the Indian Point Unit 3 intake catwalk for the Zebra Mussel Monitoring Program during 2004. Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (m-2) Species Density (m-2) Length (mm)

Mean S.D. 8Jan03 4Dec02 1 1 2 20 None 2 4 20 None 6Nov02 2 1 4 20 None 2 2 20 None 3Oct02 3 1 8 20 None 2 6 20 None 5Feb03 8Jan03 1 1 4 20 None 2 4 20 None 4Dec02 2 1 4 20 None 2 4 20 None 6Nov02 3 1 2 20 None 2 2 20 None 5Mar03 5Feb03 1 1 4 20 None 2 2 20 None 8Jan03 2 1 4 20 None 2 2 20 None 4Dec02 3 1 6 20 None 2 4 20 None 3Sep02 6 1 604 200 None 2 362 200 None 15Apr03 5Mar03 1 1 4 20 None 2 4 20 None 5Feb03 2 1 4 20 None 2 4 20 None 8Jan03 3 1 6 20 None 2 4 20 None 13May03 15Apr03 1 1 2 20 None 2 2 20 None 5Mar03 2 1 2 20 None 2 2 20 None 5Feb03 3 1 4 20 None 2 4 20 None 10Jun03 13May03 1 1 6 20 None 2 6 20 None 15Apr03 2 1 6 20 None 2 4 20 None 5Mar03 3 1 12 20 None 2 10 20 None 4Dec02 6 1 10 20 None 2 12 20 None 5Jun02 12 1 8 20 None 2 258 200 None 9Jul03 10Jun03 13May03 15Apr03 1 2 3 1 2 1 2

1 2 9.4 9.6 11.0 18.4 46.6 7.6 20 20 20 20 20 20 None DFM None DFM None ZM 60 40

20 0.6 0.4 1.0 0.2 0.1 (continued)

Table 1 (continued) Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (m-2) Species Density (m-2) Length (mm)

Mean S.D. 5Aug03 9Jul03 10Jun03 13May03 1 2 3 1 2 1 2 1 2 262.8 285.8 239.2 371.2

869.2 273.2 200 200 200 200 200 200 DFM ZM None ZM DFM None ZM DFM 200 200 4200 1400

2200 400 0.8 2.0 2.4 2.1 2.3 2.1 0.4 0.4 0.4 0.2 10Sep03 5Aug03 9Jul03 10Jun03 5Mar03 1 2 3 6 1 2 1 2 1 2 1 2 4.4

27.8 2812.2 1632.4 676.6

1693.4 930.6 525.8 20

20 200 200 200 200 200 200 ZM DFM ZM DFM ZM DFM ZM ZM DFM DFM ZM DFM DFM 20 40 1000 1080 800 1000 400 1400 2400 200 400 400 200 2.0 0.8 0.9 0.8 1.5 0.6 1.0 1.2 1.0 0.6 1.7 0.8 0.3 0.1 0.4 0.3 1.0 0.2 0.6 0.7 0.5 0.2 0.1 14Oct03 10Sep03 5Aug03 9Jul03 1 2 3 1 2 1 2 1 2 6.8 20.8 253.4 168.0 3210.6 1057.6 20 20 200 200 200 200 None ZM DFM None ZM DFM ZM DFM ZM DFM 80 20 1600 2600 1200 1000 800 800 0.4 0.3 1.1 1.0 0.7 0.9 0.4 1.4 0.2 0.8 0.4 0.4 0.3 0.2 0.3 18Nov03 14Oct03 10Sep03 5Aug03 1 2 3 1 2

1 2 1 2 3.4 2.8 33.4 25.6 220.2 165.8 20 20 20 20 200 200 None None None None DFM DFM ZM

200 2800 600

2.0 1.2 2.0

0.7 0.1 9Dec03 18Nov03 14Oct03 10Sep03 10Jun03 4Dec02 1 2 3 6

12 1 2

1 2 1 2

1 2 1 2 20.0 19.4 15.0 66.6 32.6 45.4 1140.2

1138.0 1148.2 614.6 20 20 20 20 20 20 200 200 200 200 None None None None None ZM DFM ZM DFM DFM DFM ZM

20 2400 600 1000 400 2600 200

1.0 1.4 1.0 1.1 1.9 1.6 1.8

0.5 0.6 0.6 1.3 0.8 (continued)

Footnotes for Table 1: Site codes: 1 = center of intake catwalk, in strong intake current 2 = north side of intake catwalk, in low-flow area Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present Table 2. Water quality data collected monthly at the Indian Point Unit 3 intake for the Zebra Mussel Monitoring Program during 2004. Sampling was conducted at 4 meters depth for all parameters and at the surface for temperature only.

Sampling Date Surface Temp. (C) 4-m Temp. (C) Conductivity (mho) Salinity (ppt) Dissolved Oxygen (mg/l) pH Turbidity (NTU) 20Jan04 3.0 2.5 122 0.1 13.9 7.1 14.9 12Feb04 0.8 1.0 1740 1.8 11.0 7.5 14.9 9Mar04 2.9 3.0 223 0.2 12.4 7.2 15.2 23Apr04 11.6 11.2 174 0.1 11.0 7.4 20.7 Table 3. Density, length, and biomass of bivalves on settlement plates in bioboxes sampling chlorinated service water at Indian Point for the Zebra Mussel Monitoring Program during 2004.

Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (m-2) Species Density (m-2) Length (mm)

Mean S.D. 8Jan03 4Dec02 1 3 4 2 2 20 20 None None 5Feb03 8Jan03 1 3 4 2 2 20 20 None None 5Mar03 5Feb03 1 3 4 2 2 20 20 None None 15Apr03 5Mar03 1 3 a 4 4 2 20 20 None None 6May03 1 5 b 6 b negligible negligible 40 40 None None 13May03 15Apr03 1 3 4 c 2 2 20 20 None None 10Jun03 13May03 1 3 4 5

6 2 2 negligible negligible 20 20 40 40 None None None None 9Jul03 10Jun03 1 3 4 5 6 2.4 1.8 0.6 0.8 20 20 20 20 None None None None 5Aug03 9Jul03 1 3 4 5 d 6 d 7.8 7.4 4.2 20 20 20 None None None 10Sep03 5Aug03 1 2 d 3 4 5

6 0.4 0.4 0.6 2.0 20 20 20 20 None None None None 14Oct03 10Sep03 1 3 4 5

6 2.2 2.2 5.6 1.8 20 20 20 20 None None None None 18Nov03 14Oct03 1 3 4 5 6 2.0 2.0 4.0 4.2 20 20 20 20 None None None None 10Dec03 18Nov03 1 3 4 5 6 2.6 2.2 2.6 2.8 20 20 20 20 None None None None (continued)

Footnotes for Table 3: Site codes: 3 = Unit 3 chemistry room 4 = Unit 3 central control air conditioning room 5 = Unit 2 Monitor #1 6 = Unit 2 Monitor #2 Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Water flow to the Site 3 biobox was shut off on 27Mar, so that plate was only exposed to incoming water for 22 days. b The Unit 2 biobox plates for May were collected on 6May, refrigerated, and delivered for analysis on 6Jun.

c There was no water flow to the Site 4 biobox when the 13May sample was collected.

d The Site 5 plate was collected on 12Aug, and the Site 6 plate was not collected in August.

address for Vic Nutter copy:

Mr. Vic Nutter Entergy Nuclear Operations, Inc.

440 Hamilton Avenue White Plains, New York 10601-5029 Ref. No. 20114.000 3 January 2005

Ms. Dara Gray Entergy Nuclear Northeast Indian Point 3 NPP P.O. Box 306 Buchanan, New York 10501

Dear Ms. Gray,

This report presents the December sampling results for the Indian Point Zebra Mussel Monitoring Program, conducted by Normandeau Associates for Entergy (contract 4500522928). Monthly settlement plate sampling was conducted on 15 December 2004 at Sites 1-4 (Unit 3).

Samples from Sites 5 and 6 (Unit 2) were not collected. Cumulative results for calendar year 2004 are summarized in Table 1 (Unit 3 intake plates), Table 2 (Unit 3 intake water quality), and Table 3 (Unit 3 and Unit 2 biobox plates).

At Site 1 and Site 2 in front of the Unit 3 intake, moderate numbers of zebra mussels (Dreissena polymorpha) and dark false mussels (Mytilopsis leucophaeata) were present on 6-month and 12-month exposure plates. Few of either species were present on the 2-month and 3-month exposure plates and there were none on the 1-month exposure plates. Based on this pattern, most of the settlement occurred before 20 September. Dark false mussels ranged in size from 0.7 to 6.7 mm and zebra mussels were between 1.0 and 4.1 mm. If you have any questions, please call me at 603-472-5191.

Sincerely, NORMANDEAU ASSOCIATES I NC.

B. Paul Lindsay enclosures Program Manager

Cc: Vic Nutter Mark Mattson Table 1. Density, length, and biomass of bivalves on settlement plates collected at the Indian Point Unit 3 intake catwalk for the Zebra Mussel Monitoring Program during 2004. Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 20Jan04 9Dec03 1 1 a 2 2.0 20 None 18Nov03 2 1 a 2 2.0 20 None 14Oct03 3 1 a 2 2.0 20 None 12Feb04 20Jan04 1 1 a 2 3.0 20 None 9Dec03 2 1 a 2 2.4 20 None 18Nov03 3 1 a 2 6.0 20 None 9Mar04 12Feb04 1 1 b 2 3.0 20 None 20Jan04 2 1 a 2 9.8 20 None 9Dec03 3 1 a 2 2.0 20 None 10Sep03 6 1 a 2 11.2 20 None 23Apr04 9Mar04 1 1 b 2 2.2 20 None 12Feb04 2 1 b 2 1.8 20 None 20Jan04 3 1 a 2 c 18May04 23Apr04 1 1 3.6 20 None 2 4.0 20 None 9Mar04 2 1 b 2 3.2 20 None 12Feb04 3 1 b 2 3.8 20 None 18Jun04 18May04 1 1 12.6 20 DFM 20 0.6 2 10.2 20 DFM 340 0.4 0.1 ZM 400 0.4 0.1 23Apr04 2 1 15.8 20 None 2 11.8 20 DFM 1600 0.4 0.1 ZM 1480 0.4 0.1 9Mar04 3 1 b 2 5.0 20 DFM 240 0.4 0.1 ZM 280 0.3 0.1 9Dec03 6 1 a 2 12.8 20 DFM 740 0.4 0.1 ZM 420 0.3 0.1 10Jun03 12 1 a 2 d (continued)

Table 1. (continued)

Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 22Jul04 18Jun04 1 1 127.6 20 DFM 20 2.2 2 155.2 200 None 18May04 2 1 608.0 200 None 2 152.2 200 DFM 200 2.7 ZM 2600 2.8 0.3 23Apr04 3 1 354.2 200 None 2 428.4 200 DFM 600 1.7 0.7 ZM 2200 2.9 0.5 17Aug04 22Jul04 1 1 16.6 20 ZM 80 0.3 0.1 2 19.0 20 DFM 60 0.3 0.1 ZM 120 0.4 0.1 18Jun04 2 1 470.8 200 DFM 1800 0.4 0.2 ZM 4200 0.4 0.3 2 266.6 200 DFM 600 0.8 0.8 ZM 1200 0.6 0.4 18May04 3 1 569.2 200 DFM 1200 0.7 0.8 ZM 3800 0.6 0.8 2 243.4 200 ZM 400 1.4 1.2 20Sep04 17Aug04 1 1 12.8 20 DFM 440 0.8 0.3 ZM 420 1.0 0.5 2 20.6 200 DFM 104000 0.7 0.4 ZM 24200 1.1 0.7 22Jul04 2 1 153.8 200 DFM 25400 0.6 0.3 ZM 19400 0.8 0.5 2 35.4 200 DFM 18000 0.8 0.6 ZM 10000 1.0 0.6 18Jun04 3 1 276.4 200 DFM 7000 0.6 0.3 ZM 5400 1.0 0.5 2 224.4 200 DFM 2200 0.7 0.3 ZM 1000 1.0 0.7 9Mar04 6 1 b 2 e 20Oct04 20Sep04 1 1 7.8 20 ZM 80 3.5 1.8 DFM 80 1.6 0.3 2 4.4 20 ZM 40 0.8 0.1 DFM 20 0.9 17Aug04 2 1 59.0 200 ZM 1400 1.5 0.8 DFM 1000 1.2 0.3 2 17.2 200 ZM 22000 1.7 0.9 DFM 2800 1.6 0.7 22Jul04 3 1 309.0 200 ZM 18400 2.4 1.7 DFM 4200 1.0 0.4 2 54.6 200 ZM 14000 1.7 0.9 DFM 3200 1.0 0.6 16Nov04 20Oct04 1 1 23.8 20 DFM 20 3.0 2 4.2 20 DFM 20 1.4 20Sep04 2 1 9.0 20 DFM 40 1.7 0.3 ZM 20 2.0 2 7.4 20 none 17Aug04 3 1 36.6 200 DFM 9600 1.7 0.4 ZM 11800 2.5 1.0 2 34.6 200 DFM 4000 1.8 0.7 ZM 7000 2.7 1.2 (continued)

Table 1. (continued)

Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 15Dec04 16Nov04 1 1 6.0 20 None 2 5.2 20 None 20Oct04 2 1 14.4 20 ZM 40 1.6 0.7 2 4.6 20 None 20Sep04 3 1 56.4 20 DFM 140 2.4 0.8 ZM 80 1.8 0.2 2 8.2 20 None 18Jun04 6 1 1544.0 200 DFM 2200 1.8 1.4 ZM 2000 2.0 0.9 2 332.0 200 DFM 3000 2.1 1.6 ZM 2400 2.5 0.8 9Dec03 12 1 a 2 328.8 200 DFM 2200 2.9 1.9 ZM 1200 1.4 0.3 Footnotes for Table 1: Site codes: 1 = center of intake catwalk, in strong intake current 2 = north side of intake catwalk, in low-flow area Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a On 20 January the Site 1 settling plate rack was discovered to have been carried away by ice (replacement plates could not be set).

b On 12 February a temporary settling rack was placed at Site 1, but by 9 March it also had been carried away by ice (replacement plates could not be set).

c The 3-month settlement plate at Site 2 was missing on 23 April.

d The 12-month settlement plate at Site 2 was missing on 18 June.

e The 6-month settlement plate at Site 2 was missing on 20 September.

Table 2. Water quality data collected monthly at the Indian Point Unit 3 intake for the Zebra Mussel Monitoring Program during 2004.

Sampling Date Surface Temp. (C) 4-m Temp. (C) Conductivity (mho) Salinity (ppt) Dissolved Oxygen (mg/l) pH Turbidity (NTU) 20Jan04 3.0 2.5 122 0.1 13.9 7.1 14.9 12Feb04 0.8 1.0 1740 1.8 11.0 7.5 14.9 9Mar04 2.9 3.0 223 0.2 12.4 7.2 15.2 23Apr04 11.6 11.2 174 0.1 11.0 7.4 20.7 18May04 20.3 19.9 420 0.3 7.8 7.4 13.6 18Jun04 23.5 23.8 2070 1.2 7.0 7.4 12.1 22Jul04 27.4 27.2 6970 3.8 6.5 7.5 9.9 17Aug04 25.3 25.5 2370 1.3 6.5 7.3 16.1 20Sep04 23.9 23.4 225 0.1 7.2 7.3 10.4 20Oct04 17.9 17.8 4520 3.0 7.7 7.3 8.8 16Nov04 10.9 10.9 1310 1.0 9.3 7.3 12.0 15Dec04 4.5 4.5 399 0.4 12.2 7.3 13.5 Sampling was conducted at 4 meters depth for all parameters and at the surface for temperature only.

Table 3. Density, length, and biomass of bivalves on settlement plates in bioboxes sampling chlorinated service water at Indian Point for the Zebra Mussel Monitoring Program during 2004.

Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 20Jan04 9Dec03 1 3 4 5 6 a 4.0 2.0 2.0 20 20 20 None None None 12Feb04 20Jan04 1 3 4 5 6 a 10.0 1.0 2.0 20 20 20 None None None 9Mar04 12Feb04 1 3 4 5 6 3.0 3.4 1.6 0.8 20 20 20 20 None None None None 23Apr04 9Mar04 1 3 4 5

6 4.8 5.8 2.6 7.4 20 20 20 20 None None None None 18May04 19May04 23Apr04 23Apr04 1 1 3 4 5 6 3.4 2.2 2.4 1.2 20 20 20 20 None None None None 18Jun04 18Jun04 18May04 19May04 1 1 3 4

5 6 2.0 2.2 0.4 0.8 20 20 20 20 None None None None 22Jul04 18Jun04 1 3 4 5 6 36.0 32.0 6.0 8.0 20 20 20 20 None None None None 17Aug04 22Jul04 1 3 4

5 6 a 3.0 3.2 1.4 20 20 20 None None None 20Sep04 17Aug04 1 3 4 5 a 6 a 5.0 5.6 20 20 None None 20Oct04 17Aug04 20Sep04 20Sep04 1 2 3 4 5 6 a 18.0 3.8 0.8 20 20 20 None None None 16Nov04 16Nov04 20Oct04 22Jul04 1 4 b 3 4 5 6 8.2 3.8 0.8 0.4 20 20 20 20 None None None None 15Dec04 16Nov04 1 3 4 5 a 6 a 4.4 3.6 20 20 None None (continued)

Footnotes for Table 3: Site codes: 3 = Unit 3 chemistry room 4 = Unit 3 central control air conditioning room 5 = Unit 2 Monitor #1 6 = Unit 2 Monitor #2 Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Sample scheduled for collection by plant personnel from Unit 2 was not collected.

b The Site 6 settling plate was not removed on the August, September, or October sampling dates, but flow was maintained through the biobox, so it was a valid four-month sample.

address for Vic Nutter copy (regular mail):

Mr. Vic Nutter Entergy Nuclear Operations, Inc.

440 Hamilton Avenue

White Plains, New York 10601-5029 Ref. No. 20407.000 22 December 2005

Ms. Dara Gray Entergy Nuclear Northeast Indian Point 3 NPP P.O. Box 306 Buchanan, New York 10501

Dear Ms. Gray,

This report presents the December sampling results for the Indian Point Zebra Mussel Monitoring Program, conducted by Normandeau Associates for Entergy (contract 4500522928). Monthly settlement plate sampling was conducted on 15 December 2005 at Sites 1- 4 (Unit 3) and Site 5 (Unit 2). Entergy did not retrieve the Site 6 settlement plate in December. Cumulative results for calendar year 2005 are summarized in Table 1 (Unit 3 intake plates), Table 2 (Unit 3 intake water quality), and Table 3 (Unit 3 and Unit 2 biobox plates). Zebra mussels (Dreissena polymorpha) and dark false mussels (Mytilopsis leucophaeata) were present in modest numbers on settling plates at Unit 3, mostly on plates that had been exposed for at least six months (Table 1). The maximum density was 2,800 per square meter for the two species combined, on the 12-month plate at Site 1. The largest zebra mussel observed was a 3.2-mm specimen on the six-month plate at Site 2. The largest dark false mussel was also 3.2 mm long and from a six-month settlement plate. No mussels were present on the biobox settling plates at Unit 3 or Unit 2. If you have any questions, please call me at 603-472-5191.

Sincerely, NORMANDEAU ASSOCIATES I NC.

B. Paul Lindsay enclosures Program Manager Cc: Vic Nutter Mark Mattson Table 1. Density, length, and biomass of bivalves on settlement plates collected at the Indian Point Unit 3 intake catwalk for the Zebra Mussel Monitoring Program during 2005. Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 8Feb05 15Dec04 2 1a,b 2 a 4.2 20 None 1 2.4 20 None 2 8.0 20 None 16Nov04 3 1a,b 2 a 282.0 20 None 1 18.0 20 None 2 3.2 20 None 20Oct04 4 1 a 3.4 20 None 2 a 2.4 20 None 10Mar05 8Feb05 1 1 9.0 20 None 2 4.8 20 None 1 c 2.2 20 None 2 c 3.0 20 None 15Dec04 3 1 b 2 6.0 20 None 20Sep04 6 1 b 2 18.4 20 None 13Apr05 10Mar05 1 1 9.4 20 None 2 5.6 20 None 8Feb05 2 1 8.0 20 None 2 5.2 20 None 1 c 8.0 20 None 2 c 3.8 20 None 11May05 13Apr05 1 1 2.8 20 None 2 8.8 20 None 10Mar05 2 1 14.4 20 None 2 7.8 20 None 8Feb05 3 1 7.4 20 None 2 7.0 20 None 15Jun05 11May05 1 1 27.4 20 None 2 29.8 20 None 13Apr05 2 1 33.4 20 None 2 24.8 20 None 10Mar05 3 1 55.6 20 None 2 42.6 20 None 20Sep04 6 1 28.0 20 None 2 42.0 20 DFM 20 0.7 18Jun04 12 1 96.2 200 None 2 44.4 20 None 13Jul05 15Jun05 1 1 443.0 200 None 2 182.4 200 DFM 200 1.0 11May05 2 1 688.4 200 DFM 200 2.2 2 232.4 200 None 13Apr05 3 1 781.8 200 None 2 322.2 200 DFM 400 0.6 0.2 (continued)

Table 1. (continued) Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 10Aug05 13Jul 05 1 1 552.6 200 ZM 2600 0.4 0.1 DFM 1800 0.2 0.1 2 738.8 200 ZM 600 0.6 0.1 DFM 2600 0.4 0.3 15Jun05 2 1 2004.8 200 ZM 1000 0.4 0.1 DFM 2600 0.4 0.1 2 1128.6 200 ZM 200 1.8 DFM 600 3.5 3.4 11May05 3 1 1627.8 200 ZM 22600 0.3 0.1 DFM 14800 0.5 0.7 2 735.0 200 ZM 1600 3.2 2.5 DFM 1400 1.5 0.8 14Sep05 10Aug05 1 1 1841.0 200 ZM 600 0.6 0.1 DFM 4600 0.5 0.3 2 904.0 200 None 13Jul 05 2 1 2159.2 200 ZM 2400 0.7 0.3 DFM 10400 0.9 0.3 2 1525.2 200 ZM 200 2.5 DFM 200 0.7 15Jun05 3 1 3268.0 200 DFM 1200 2.8 1.2 2 1566.0 200 ZM 200 3.2 DFM 400 1.2 0.4 10Mar05 6 1 3187.2 200 DFM 1800 2.5 1.6 2 1529.6 200 DFM 600 0.8 0.2 17Oct05 14Sep05 1 1 204.6 200 ZM 600 1.0 0.6 DFM 1200 0.6 0.1 2 60.8 20 None 10Aug05 2 1 3140.2 200 ZM 200 1.6 DFM 200 1.2 2 2058.0 200 None 13Jul 05 3 1 2746.4 200 ZM 1800 0.8 0.1 DFM 2400 0.9 0.4 2 1386.0 200 DFM 1000 1.0 0.3 9Nov05 17Oct05 1 1 8.6 20 None 2 2.0 20 None 14Sep05 2 1 185.2 20 ZM 40 1.9 0.2 DFM 60 1.0 0.6 2 133.0 20 None 10Aug05 3 1 4184.8 200 DFM 400 0.6 0.1 2 2231.6 200 ZM 200 1.3 DFM 400 1.0 0.3 15Dec05 9Nov05 1 1 18.0 20 None 2 14.2 20 None 17Oct05 2 1 28.0 20 None 2 24.0 20 None 14Sep05 3 1 281.8 200 None 2 2921.4 200 DFM 400 1.1 0.1 15Jun05 6 1 3902.4 200 ZM 800 1.7 0.9 DFM 1800 1.5 0.8 2 2578.0 200 ZM 400 1.9 1.8 DFM 600 0.7 0.2 15Dec04 12 1 3060.2 200 ZM 1000 1.4 0.4 DFM 1800 1.7 0.7 2 1208.4 200 ZM 200 1.1 DFM 600 0.9 0.1 (continued)

Footnotes for Table 1: Site codes: 1 = center of intake catwalk, in strong intake current 2 = north side of intake catwalk, in low-flow area Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Retrieval postponed one month due to excessive ice in January b Plate missing due to ice damage c Set postponed one month due to excessive ice in January

Table 2. Water quality data collected monthly at the Indian Point Unit 3 intake for the Zebra Mussel Monitoring Program during 2005.

Sampling Date Surface Temp. (C) 4-m Temp. (C) Conductivity (mho) Salinity (ppt) Dissolved Oxygen (mg/l) pH Turbidity (NTU) 8Feb05 2.3 2.5 2130 2.0 13.4 7.4 35.5 10Mar05 2.4 2.5 390 0.4 13.1 7.5 22.0 13Apr05 11.1 10.9 166 0.1 11.0 7.4 59.5 11May05 16.4 16.3 300 0.2 8.6 7.4 14.5 15Jun05 23.9 23.8 1030 0.6 7.7 7.4 7.0 13Jul05 26.6 26.6 4790 2.6 5.9 7.4 9.2 10Aug05 30.3 30.2 7090 3.6 5.5 7.4 8.1 14Sep05 27.2 27.2 8210 4.5 6.2 7.5 8.1 17Oct05 17.2 16.9 301 0.2 8.5 7.4 15.1 9Nov05 10.5 10.1 181 0.1 9.2 7.4 10.3 15Dec05 3.9 4.0 127 0.1 12.0 7.4 31.5 Sampling was conducted at 4 meters depth for all parameters and at the surface for temperature only. No water quality data were collected in January due to excessive ice.

Table 3. Density, length, and biomass of bivalves on settlement plates in bioboxes sampling chlorinated service water at Indian Point for the Zebra Mussel Monitoring Program during 2005.

Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 26Jan05 15Dec04 16Nov04 1 2 3 4

5 6 3.0 4.0 0.4 3.0 20 20 20 20 None None None None 8Feb05 26Jan05 1 3 4 5 a 6 a 16.0 6.0 20 20 None None 10Mar05 8Feb05 26Jan05 1 2 3 4 5 6 10.6 12.6 4.2 9.6 20 20 20 20 None None None None 13Apr05 10Mar05 1 3 4 5

6 8.2 9.4 18.2 11.8 20 20 20 20 None None None None 11May05 13Apr05 1 3 4 5 6 b 1.0 1.6 1.4 20 20 20 None None None 15Jun05 11May05 1 3 4 c 5 6 b 4.0 1.8 20 20 None None 13Jul05 15Jun05 1 0.75 3 4 c 5 a 6 a 6.6 6.4 20 20 None None 10Aug05 13Jul05 1 2 3 4

5 6 a 4.8 1.8 9.8 20 20 20 None None None 14Sep05 10Aug05 1 3 4 5 6 1.8 3.4 10.6 13.0 20 20 20 20 None None None None 17Oct05 14Sep05 1 3 4 5 6 a 2.2 1.0 7.6 20 20 20 None None None 9Nov05 16Nov05 17Oct05 17Oct05 0.75 1 3 4 5 6 a 18.4 1.0 1.6 20 20 20 None None None 15Dec05 15Dec05 9Nov05 16Nov05 1.25 1 3 4 5 6 a 5.8 11.6 4.6 20 20 20 None None None (continued)

Footnotes for Table 3: Site codes: 3 = Unit 3 chemistry room 4 = Unit 3 central control air conditioning room 5 = Unit 2 Monitor #1 6 = Unit 2 Monitor #2 Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Sample scheduled for collection by Entergy personnel from Unit 2 was not collected.

b Site 6 sample was not collected in May or June because the biobox was clogged and there was no flow through it.

c Site 4 sample could not be processed in June because the biobox had gone dry. Flow was restored 22 June.

address for Vic Nutter copy (regular mail):

Mr. Vic Nutter Entergy Nuclear Operations, Inc.

440 Hamilton Avenue White Plains, New York 10601-5029 Ref. No. 20407.001 20 December 2006 (sent by e-mail)

Ms. Dara Gray Entergy Nuclear Northeast Indian Point 3 NPP P.O. Box 306 Buchanan, New York 10501

Dear Ms. Gray,

This report presents the December sampling results for the Indian Point Zebra Mussel Monitoring Program, conducted by Normandeau Associates for Entergy (contract 4500522928). Monthly settlement plate sampling was conducted on 12 December 2006 at Sites 1- 4 (Unit 3). Entergy did not retrieve the Site 5 or Site 6 settlement plates due to a lack of flow to the bioboxes, which cannot be rectified until there is a plant outage. Cumulative results for calendar year 2006 are summarized in Table 1 (Unit 3 intake plates), Table 2 (Unit 3 intake water quality), and Table 3 (Unit 3 and Unit 2 biobox plates).

No zebra mussels (Dreissena polymorpha) or dark false mussels (Mytilopsis leucophaeata) were observed on 1-month, 2-month, or 3-month settling plates collected in front of the Unit 3 intake in December, but both species were present in low numbers on settling plates that had been exposed for 6 months and 12 months. The observed densities on the 6-month plates and 12-month plates were low compared to densities observed in August and September. The lack of settled mussels on the plates exposed for three months or less and the sizes of the mussels on the plates exposed for 6 and 12 months (0.5-2.2 mm) indicate that the mussels had not settled recently. There were no settled mussels on the biobox plates from Unit 3. If you have any questions, please call me at 603-472-5191.

Sincerely, NORMANDEAU ASSOCIATES I NC. B. Paul Lindsay Program Manager Cc: Vic Nutter Mark Mattson Table 1. Density, length, and biomass of bivalves on settlement plates collected at the Indian Point Unit 3 intake catwalk for the Zebra Mussel Monitoring Program during 2006. Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 11Jan06 15Dec05 1 1 7.8 20 None 2 4.2 20 None 9Nov05 2 1 11.4 20 None 2 4.4 20 None 17Oct05 3 1 10.4 20 None 2 11.2 20 None 15Feb06 11Jan06 1 1 3.0 20 None 2 5.8 20 None 15Dec05 2 1 3.6 20 None 2 8.2 20 None 9Nov05 3 1 8.2 20 None 2 9.2 20 None 15Mar06 15Feb06 1 1 20.2 20 None 2 17.2 20 None 11Jan06 2 1 15.4 20 None 2 1.2 20 None 15Dec05 3 1 26.6 20 None 2 17.8 20 None 14Sep05 6 1 a 2 87.6 20 None 13Apr06 15Mar06 1 1 13.6 20 None 2 6.8 20 None 15Feb06 2 1 4.8 20 None 2 12.2 20 None 11Jan06 3 1 6.2 20 None 2 10.4 20 None 17May06 13Apr06 1 1 8.4 20 None 2 6.8 20 None 15Mar06 2 1 8.6 20 None 2 2.4 20 None 15Feb06 3 1 7.8 20 None 2 5.6 20 None 14Jun06 17May06 1 1 22.8 20 None 2 8.4 20 None 13Apr06 2 1 10.6 20 None 2 15.8 20 None 15Mar06 3 1 26.0 20 None 2 12.8 20 None 15Dec05 6 1 22.4 20 None 2 17.6 20 DFM 20 0.3 15Jun05 12 1 1402.0 200 DFM 600 1.9 0.3 2 a 10Jul06 14Jun06 1 1 12.2 20 None 2 7.8 20 ZM 40 0.4 0.1 17May06 2 1 23.0 20 ZM 80 0.8 0.1 DFM 140 0.6 0.2 2 15.4 20 ZM 80 0.7 0.3 DFM 40 0.6 0.1 13Apr06 3 1 119.0 20 ZM 760 0.7 0.2 DFM 320 0.6 0.2 2 57.0 20 ZM 200 0.8 0.1 DFM 100 0.6 0.1 (continued)

Table 1 (continued) Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 25Aug06 10Jul06 1 1 405.6 200 ZM 1000 0.4 0.2 2 27.0 20 ZM 60 0.8 0.4 14Jun06 2 1 612.2 200 ZM 400 0.6 0.1 DFM 600 2.3 2.3 2 184.0 200 ZM 2200 0.5 0.2 DFM 1800 0.3 0.1 17May06 3 1 1124.2 200 ZM 600 0.4 0.1 DFM 400 0.8 0.1 2 194.6 200 ZM 1600 1.0 1.3 DFM 1800 0.3 0.3 26Sep06 25Aug06 1 1 185.2 200 None 2 87.6 20 None 10Jul06 2 1 707.0 200 ZM 1600 0.6 0.3 DFM 1000 0.4 0.2 2 625.0 200 ZM 2000 0.6 0.2 DFM 1400 0.4 0.2 14Jun06 3 1 682.0 200 ZM 800 0.6 0.2 DFM 200 0.5 2 1267.4 200 ZM 1600 0.5 0.2 DFM 1000 0.3 0.1 15Mar06 6 1 1614.2 200 ZM 400 1.2 1.0 DFM 800 0.7 0.3 2 1128.6 200 ZM 1400 0.7 0.4 DFM 1000 0.6 0.6 18Oct06 26Sep06 1 1 17.2 20 None 2 11.0 20 None 25Aug06 2 1 998.6 200 ZM 400 0.5 0.2 DFM 600 0.6 0.1 2 237.0 200 None 10Jul06 3 1 1709.4 200 ZM 600 1.0 0.1 DFM 200 1.1 2 593.8 200 ZM 200 0.5 DFM 400 0.9 0.1 15Nov06 18Oct06 1 1 12.8 20 None 2 5.8 20 None 26Sep06 2 1 12.2 20 None 2 10.8 20 None 25Aug06 3 1 2574.0 200 DFM 200 0.6 2 300.2 200 None 12Dec06 15Nov06 1 1 6.0 20 None 2 6.4 20 None 18Oct06 2 1 7.0 20 None 2 9.6 20 None 26Sep06 3 1 32.0 20 None 2 5.4 20 None 14Jun06 6 1 2560.2 200 ZM 400 1.7 0.7 DFM 600 0.6 0.1 2 914.4 200 ZM 1200 1.1 0.2 DFM 600 1.0 0.4 15Dec05 12 1 4347.2 200 ZM 800 1.0 0.4 DFM 400 0.8 0.1 2 2020.0 200 ZM 200 1.7 DFM 200 1.5 Footnotes for Table 1: Site codes: 1 = center of intake catwalk, in strong intake current 2 = north side of intake catwalk, in low-flow area Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Plate missing Table 2. Water quality data collected monthly at the Indian Point Unit 3 intake for the Zebra Mussel Monitoring Program during 2006.

Sampling Date Surface Temp. (C) 4-m Temp. (C) Conductivity (mho) Salinity (ppt) Dissolved Oxygen (mg/l) pH Turbidity (NTU) 11Jan06 3.3 3.2 154 0.1 12.5 7.3 20.9 15Feb06 3.2 3.2 920 0.9 13.0 7.5 18.6 15Mar06 3.8 3.7 1519 1.4 11.5 7.3 18.4 13Apr06 10.0 9.9 3190 2.5 10.1 7.3 18.1 17May06 15.9 15.7 2550 1.7 8.8 7.4 19.1 14Jun06 20.9 20.8 305 0.2 7.7 7.4 17.9 10Jul06 23.9 23.5 1325 0.8 6.1 7.4 18.9 25Aug06 27.3 27.2 7979 4.4 7.0 7.4 7.8 26Sep06 23.3 23.4 4190 2.4 6.5 7.4 10.1 18Oct06 19.0 18.9 2610 1.7 6.8 7.3 7.5 15Nov06 11.6 11.5 605 0.5 10.6 7.4 14.5 12Dec06 8.6 8.5 137 0.1 10.6 7.3 24.9 Sampling was conducted at 4 meters depth for all parameters and at the surface for temperature only.

Table 3. Density, length, and biomass of bivalves on settlement plates in bioboxes sampling chlorinated service water at Indian Point for the Zebra Mussel Monitoring Program during 2006.

Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 11Jan06 15Dec05 1 3 4 5 6 a 2.8 4.4 3.2 20 20 20 None None None 15Feb06 11Jan06 1 3 4 5 6 a 7.2 9.0 2.4 20 20 20 None None None 15Mar06 15Feb06 1 3 4 5 a 6 a 4.2 4.0 20 20 None None 13Apr06 15Mar06 1 3 4 5 a 6 a 0.6 2.6 20 20 None None 17May06 13Apr06 1 3 4 5 a 6 a 3.6 2.2 20 20 None None 14Jun06 17May06 1 3 4 5 a 6 a 1.0 1.6 20 20 None None 10Jul06 14Jun06 1 3 4 5 a 6 a 5.2 5.6 20 20 None None 25Aug06 10Jul06 1 3 4 5 a 6 a 3.8 6.6 20 20 None None 26Sep06 25Aug06 1 3 4 5 a 6 a 2.4 3.2 20 20 None None 18Oct06 26Sep06 1 3 4 5 a 6 a 4.6 2.2 20 20 None None 15Nov06 18Oct06 1 3 4 5 a 6 a 4.8 1.8 20 20 None None (continued)

Table 3. (Continued)

Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm) 12Dec06 15Nov06 1 3 4 5 a 6 a 8.2 12.0 20 20 None None Footnotes for Table 3:

Site codes: 3 = Unit 3 chemistry room 4 = Unit 3 central control air conditioning room 5 = Unit 2 Monitor #1 6 = Unit 2 Monitor #2 Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Sample scheduled for collection by Entergy personnel from Unit 2 was not collected.

Ref. No. 20407.003 5 January 2009 (sent by e-mail)

Ms. Dara Gray Entergy Nuclear Northeast Indian Point 3 NPP P.O. Box 306 Buchanan, New York 10501

Dear Ms. Gray,

This report presents the December sampling results for the Indian Point Zebra Mussel Monitoring Program, conducted by Normandeau Associates for Entergy (contract 10150496). Monthly settlement plate sampling was conducted on 18 December 2008 at Sites 1 and 2 (Unit 3 intake). The settlement plates at Sites 3 and 4 (Unit 3 bioboxes) were collected, although the water flow had been shut off some time before they were collected. Entergy did not retrieve the settlement plates at Sites 5 and 6 (Unit 2 bioboxes) due to a lack of flow to the bioboxes. Cumulative results for calendar year 2008 are summarized in Table 1 (Unit 3 intake plates), Table 2 (Unit 3 intake water quality), and Table 3 (Unit 3 and Unit 2 biobox plates). In December, zebra mussels (Dreissena polymorpha) and dark false mussels (Mytilopsis leucophaeata) were observed in low numbers on the three-month, six-month, and twelve-month settlement plates from Site 2 at the Unit 3 intake. Densities ranged from 200 to 2,200 zebra mussels and from 200 to 2,000 dark false mussels per square meter, with the lowest densities on the three-month plates. Sizes ranged from 0.4 to 6.5 mm for zebra mussels and from 0.5 to 6.7 for dark false mussels, with 58% of ze bra mussels and 57% of false dark mussels 1.0 mm. No settled mussels were present on exposure plates from the Unit 3 bioboxes. If you have any questions, please contact me by e-mail at plindsay@normandeau.com or by phone at 603-637-1149.

Sincerely, NORMANDEAU ASSOCIATES I NC. B. Paul Lindsay Program Manager

Cc: Rick Buckley Mark Mattson Table 1. Density, length, and biomass of bivalves on settlement plates collected at the Indian Point Unit 3 intake catwalk for the Zebra Mussel Monitoring Program during 2008. Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 30Jan08 18Dec07 1 1 a 2 5.2 20 None 19Nov07 2 1 a 2 5.0 20 None 22Oct07 3 1 a 2 15.0 20 None 21Feb08 30Jan08 1 2 b 18Dec07 2 1a,b 2 b 19Nov07 3 1a,b 2 b 22Oct07 4 1a,b 26Mar08 30Jan08 2 2 c 23.0 20 None 2 2.6 20 None 18Dec07 3 1 d 55.1 20 None 1 c 15.0 20 None 1 57.0 20 None 2 c 11.6 20 None 2 5.0 20 None 19Nov07 4 1 d 17.4 20 None 1 c 20.6 20 None 2 c 1.6 20 None 22Oct07 5 1 d 40.4 20 None 12Sep07 6 1 1462.0 200 None 2 1977.2 200 None 15Apr08 26Mar08 1 1 9.0 20 None 2 14.8 20 None 1 e 5.0 20 None 2 e 9.4 20 None 1 f 5.8 20 None 30Jan08 3 2 937.0 200 None 15May08 15Apr08 1 1 3.2 20 None 2 8.2 20 None 26Mar08 2 1 4.6 20 None 2 5.6 20 None 1 e 5.4 20 None 2 e 5.2 20 None 17Jun08 15May08 1 1 39.0 20 None 2 11.8 20 None 15Apr08 2 1 8.8 20 None 2 11.6 20 None 26Mar08 3 1 8.0 20 None 2 21.6 20 None 18Dec07 6 1 50.6 20 None 2 10.8 20 None 15Jun07 12 1 2068.0 200 None 2 1207.0 200 None (continued)

Table 1. (Continued) Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 16Jul08 17Jun08 1 1 224.2 200 None 2 206.4 200 None 15May08 2 1 629.4 200 None 2 313.2 200 None 15Apr08 3 1 g 2 298.0 200 ZM 400 0.6 0.1 DFM 200 2.7 13Aug08 16Jul08 1 1 44.8 20 ZM 400 0.6 0.2 DFM 260 0.4 0.2 2 47.6 20 ZM 1700 0.5 0.3 DFM 1140 0.4 0.2 17Jun08 2 1 1451.2 200 ZM 8200 0.5 0.2 DFM 6400 0.5 0.3 2 411.6 200 ZM 6200 0.5 0.3 DFM 5600 0.4 0.2 15May08 3 1 104.2 200 ZM 800 0.8 0.3 DFM 1000 0.5 0.2 2 625.2 200 ZM 2800 0.4 0.2 DFM 1800 0.5 0.2 15Sep08 13Aug08 1 1 g 2 85.4 20 ZM 740 0.7 0.5 DFM 820 0.5 0.4 16Jul08 2 1 g 2 298.6 200 ZM 3400 0.7 0.4 DFM 5400 0.7 0.4 17Jun08 3 1 g 2 907.2 200 ZM 2000 0.8 0.5 DFM 2600 0.8 0.6 26Mar08 6 1 g 2 548.8 200 ZM 2200 0.6 0.5 DFM 3600 0.6 0.4 16Oct08 24Sep08 1 1 8.0 20 None 2 94.6 20 DFM 20 0.6 13Aug08 2 1 g 2 486.8 200 ZM 800 0.5 0.1 DFM 400 0.9 0.4 16Jul08 3 1 g 2 3468.2 200 ZM 600 0.8 0.3 DFM 1000 0.9 0.4 12Nov08 16Oct08 1 1 0.4 20 None 1 h 0.6 20 None 2 5.4 20 None 24Sep08 2 1 0.6 20 None 2 254.2 200 ZM 200 1.2 DFM 400 0.8 0.4 13Aug08 3 2 1817.8 200 ZM 800 0.7 0.4 DFM 2000 0.9 0.3 (continued)

Table 1. (Continued) Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 18Dec08 12Nov08 1 1 1.0 20 None 2 8.8 20 None 16Oct08 2 1 1.6 20 None 1 h 1.2 20 None 1 h 1.6 20 None 2 7.6 20 None 24Sep08 3 1 7.2 20 None 2 273.8 200 ZM 200 1.2 DFM 200 5.7 17Jun08 6 2 1137.6 200 ZM 1400 1.8 2.2 DFM 2400 2.0 1.8 18Dec07 12 2 2679.4 200 ZM 2200 1.0 0.4 DFM 1600 0.8 0.3 Footnotes for Table 1: Site codes: 1 = center of intake catwalk, in strong intake current 2 = north side of intake catwalk, in low-flow area Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Not collected: access was denied due to security restrictions b Not collected: guard was not able to open the frozen lock c Collection delayed 1 month due to missed February collection d Collection delayed 2 months due to missed January and February collections e Set was delayed 1 month due to missed February collection f Set was delayed 2 months due to missed January and February collections g Not collected: the entire Site 1 settlement plate rack was missing on 15Sep h Duration was shortened due to missing Site 1 settlement plate rack on 15Sep (plate was reset on 16Oct)

Table 2. Water quality data collected monthly at the Indian Point Unit 3 intake for the Zebra Mussel Monitoring Program during 2008.

Sampling Date Surface Temp. (C) 4-m Temp. (C) Conductivity (mho) Salinity (ppt) Dissolved Oxygen (mg/l) pH Turbidity (NTU) 30Jan08 3.4 3.4 910 0.8 12.4 7.4 17.5 26Mar08 6.1 5.9 140 0.1 12.4 7.4 22.5 15Apr08 9.8 9.2 195 0.2 11.1 7.4 15.5 15May08 16.0 15.8 138 0.1 8.5 7.4 14.5 17Jun08 24.2 24.1 6405 3.7 8.5 7.4 9.5 16Jul08 27.4 27.4 6650 3.6 7.6 7.4 5.1 13Aug08 26.3 26.2 3690 2.0 5.8 7.4 8.9 15Sep08 25.9 26.0 5300 2.9 5.5 7.4 9.3 16Oct08 20.5 20.5 9700 6.1 6.5 7.4 9.1 12Nov08 12.4 12.4 4990 3.7 9.6 7.4 9.1 18Dec08 4.0 4.0 130 0.1 11.1 7.4 9.1 Sampling was conducted at 4 meters depth for all parameters and at the surface for temperature only. No sampling was conducted at the Unit 3 intake in February because the guard could not open the locked gate.

Table 3. Density, length, and biomass of bivalves on settlement plates in bioboxes sampling chlorinated service water at Indian Point for the Zebra Mussel Monitoring Program during 2008.

Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 30Jan08 18Dec07 1 3 4 5 a 6 a 3.8 4.4 20 20 None None 21Feb08 30Jan08 1 3 4 5 a 6 a 0.6 0.8 20 20 None None 26Mar08 21Feb08 1 3 4 5 a 6 a 2.4 2.0 20 20 None None 15Apr08 26Mar08 1 3 4 5 a 6 a 1.2 0.8 20 20 None None 15May08 15Apr08 1 3 4 5 a 6 a 1.2 0.6 20 20 None None 17Jun08 15May08 1 3 4 5 a 6 a 0.8 0.6 20 20 None None 16Jul08 17Jun08 1 3 4 5 a 6 a 2.2 12.0 20 20 None None 13Aug08 16Jul08 1 3 4 5 a 6 a 2.8 3.4 20 20 None None 15Sep08 13Aug08 1 3 4 5 a 6 a 1.4 18.0 20 20 None None 15Oct08 15Sep08 1 3 4 5 a 6 a 1.0 1.0 20 20 None None 12Nov08 15Oct08 1 3 4 5 a 6 a 0.6 2.8 20 20 None None (continued)

Table 3. (Continued) Date Collected Date Set Exposure Duration (Months) Site Biomass (g/m 2) Detection Limit (no./m 2) Species Density (no./m 2) Length (mm)

Mean S.D. 18Dec08 12Nov08 1 3 b 4 b 5 a 6 a 1.0 0.6 20 20 None None Footnotes for Table 3: Site codes: 3 = Unit 3 chemistry room 4 = Unit 3 central control air conditioning room 5 = Unit 2 Monitor #1 6 = Unit 2 Monitor #2 Species codes: ZM = zebra mussel (Dreissena polymorpha) DFM = dark false mussel (Mytilopsis leucophaeata) OB = other bivalves (including unidentified because of damage) None = No bivalves present a Sample scheduled for collection by Entergy personnel from Unit 2 was not collected.

b Sample was collected, but there was no flow to the biobox at the time of collection.