ML17163A414

Submittal of Discharge Monitoring Report for May 2017

ML17163A414
Person / Time
Site:	Sequoyah
Issue date:	06/08/2017
From:	Williams A L Tennessee Valley Authority
To:	Document Control Desk, Office of Nuclear Reactor Regulation
References
TN0026450
Download: ML17163A414 (101)

Text

{{#Wiki_filter:Tennessee Va ll ey Aut hority , Post Office Box 2000 , So dd y Daisy, Te nne ss ee 37384-2000 June 8 , 2017 Chattanooga Environmental Field Office D i vision of Water Pollution Control 1301 Riverfront Parkway , #206 Chattanooga , Tennessee 37402-2013 TENNESSEE VALLEY AUTHORITY (TVA) -SEQUOYAH NUCLEAR PLANT (SQN) -NPDES PERMIT NO. TN0026450 -DISCHARGE MONITORING REPORT (DMR) FOR May 2017 Enclosed i s the May 2017 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period. The final report for toxic i ty testing conducted at outfall 101 , April 30 -May 5 , 2017 , is enclosed. No adverse effects were observed. If you have any quest i ons or need add i t i onal information, p l ease contact Millicent Garland by email at mrmoore@tva .gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the informat ion , the information submitted is , to the best of my knowledge and bel i ef, true , accurate , and comple te. I am aware that there are significant penalties for submitting false information, including the possib ility of fine and impr isonment for know i ng viola ti ons. ony L. W i lliams Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosu r es): U.S. Nuclear Regulatory Commiss i on Attn: Document Control Desk Washington , DC 20555 P ERM ITTEE NA M E/AD D R E SS (Include Facil i ty Name/L ocati o n if Di ff erent) Na me _ ___ _ Addre s.L ___________ _ ___ _il NTER OFFICE OPS-5 N-SQ!!l _______ _ _ _ _ __§O D DY -DAISY .._I.N_273B4_ _______ _ Fa c J!!ly _ _I VA-_fil:QUOYA H NU CL EAR P LAN T _____ _ L o c a t i o.!]_ _l!AMIL TOJi.C OUNTL_ _________ _ A TI N: M illic e nt Gar l a n d NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NP DESJ DISCHARGE MONITORING REPORT (O M R) TN0026450 10 1 G PERMIT NUMBER DISCHARGE NUMBER AR M O D YE MO DAY 1 MAJOR (SUBR 0 1) F -FINAL D I FFUSER DISCHARGE EFFLUENT *** NO DISCHARGE D ... Form A ppr o ved. OMB No. 2 040-0004 F r om ;7 I 05 01 : To [ 17 : 05 I J i NOTE: Re ad i nstruc t i on s b e fore completinQ thi s f o rm. PARAMETER i>< QUANTITY OR L OADING QUALITY OR CONCENTRATION NO. FREQU EN CY SAMPLE EX OF TYPE AV ERAGE MAXIM U M U N IT S MI NIMUM AVERAG E MAXIMUM UN I TS A N A L YSI S TEMPERATURE, WATER DEG. S AMPL E ******** ******** ******** ******** 35.6 0 31 / 31 RCORDR ** 04 CENTIGRADE M E ASUREMEN T 00010 1 0 PERM IT ******** ******** **** ******** ******** Req. Mon. DEG.C. CONT I CALCTD EFFLUENT GROSS R E QU I REMENT DAILY MAX NUOUS TEMPERATURE , WATER DEG. SAMPL E ******** ******** ******** ******** 23.4 0 3 1 I 31 MODELO ** 04 CENTIGRADE MEA SUREMEN T 00010 z 0 PERMIT ******** ******** **** ******** ******** 30.5 DEG.C . CONTI CALCTD INSTREAM MON I TORING REQUIREMENT DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & S AMP LE ******** ******** ******** ******** 1.6 0 31 / 31 CALCTD .. 04 UPSTRM DEG.C M E ASUREME NT 000 1 6 1 s P E RMIT ******** ******** .... ******** ******** 3.0 DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** ******** 0 31 I 31 RCORDR /908 03 .. TREATMENT PLANT M EA SUREM E N T 50050 1 0 PERMIT ******it* Req. Mon. MGD ******** ******** ******** **** CONTI RCORDR EFF L UENT GROSS R E QU I REMENT DAILY MAX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1 433 ******** ******** ********

• • • • • • • • 0 31 I 31 CALCTD TREATMENT PLANT M E ASUREMENT 03 03 50050 1 0 PERM I T Req. Mon. ******** MGD ******** ******** ******** MGD CONTI CALCTD EFF L UENT GROSS VALUE R E QUIREMENT MO AVG NUOUS CHLORINE , TOTAL RESIDUAL SAMPL E ******** ******** ******** 0.018 0.036 0 20 / 31 GRAB MEASUREMEN T .. 19 50060 1 0 PERMIT ******** ******** .... ******** 0.1 0.1 MG/L FIVE PER CALCTD EFFLUENT GROSS VALUE R E QUIREMEN T MO AVG DAILY MAX WEEK TEMPERATURE

-C, RATE OF SAMPLE ******** 0.2 ******** ******** 0 31/31 CALCTD 62 .. CHANGE M E ASUREMENT 82234 1 0 PERM I T ******** 2.0 DEG ******** ******** ******* **** CONTI CALCTD EFFLUENT GROSS R E QUIREMENT C/HR _,/" -----)--------*- NUOUS DAILY MX *-/ NAME/TIT LE PR I NC I PA L E XECU TI V E OF FI CER ' ""'""" ,.,,.,," ,_ '"" '" """"'"' '"' ., "'""--""'"' "¥. _/ TELEPHONE D A TE d i rec ti on or superv ision in accordance with a system des i gned lo assure thal qua li fied Anthony L. W illi ams personnel proper ly galher and evaluate the in format ion submilled. Based on my i nquiry o the P r e:d e n t p erso n or persons who manage the system , or those persons dire ctly r esponsible for gal er ing lhe info rmat ion , the in f orma l io n subm i lled is , to th e best of my knowledge and belief , true , 423 8 43-7 0 01 17 06 0 7 S ite V ice President accurate , and complele. I am aware th at there are significant pena l t i es for submi tl i ng fa lse SIGNATURE O F PRINCIPAL EXECUTIVE I infor mation , i nc luding the possibili ty of fine and i mpr i sonment f or knowing v iol ations. OFFIC ER O R AUTHO R IZED A GE N T AREA I NUMBER YE AR MO D A Y TY PED O R P R I N TED CO DE CO MM E N TS AND E X P L ANA TIO N O F ANY VIO LAT I O NS (R e f e r e n ce a ll a tt ac h me nts he r e) No c lose d m o d e operation. T h e following injecti ons o ccu r red: Spectrus CT 13 00 (max calc. was 0.0 39 mg/ml, limit 0.0 5 mg/L), Spe c t rus BO 15 00 (max ca lc. was 0.046 m g fml, limit -2.0 mg/ml). EPA For m 3320-1 (R EV 3199) Previous editions ma y be used Pf!O P. 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if D i fferen t) ___ _ Addres_L

---

___ .J!NTEROFFICE OPS-5N-SQNl _______ _ NATIONAL POLLUTANT DIS C HARGE ELIMINATION SYS TE M (NPDES J DISCHARGE MONITORING REPORT (DMR) TN0026450 101 T MAJOR (SUBR 01) F -FINAL Form Approved. OMB No. 2040-0004

_ _ _ _§ODDY -DAISY J.N_..]7381._ _______ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Fac_ill!y_ ..JYA-SEQUOYAH NUCLEAR PLANT _____ _ LocatiO.!L _!!AMIL TOJi.COUNTY._ _________ _ ATIN: Millicent Garland PARAMETER >< QUANTITY OR LOADING AVERAGE MAXIMUM IC25 STATRE 7DAY CHR SAMPLE ******** ******** CERIODAPHNIA MEASUREMENT TRP38 1 0 PERMIT ******** ******** EFFLUENT GROSS REQUIREMENT IC25 STATRE 7DAY CHR SAMPLE ******** ******** PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT ******** ******** EFFLUENT GROSS REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT UNITS MINIMUM ** >100.0 .... 42.8 MINIMUM ** >100.0 .... 42.8 MIMINUM DAY 31 EFFLUENT NO DISCHARGE D ... NOTE: Read i nstructions before complet i ni:i th i s fo r m. QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS ******** ******** 0 1/180 COMPOS 23 ******** ******** PERCENT SEMI COMPOS ANNUAL ******** ******** 0 1/180 COMPOS 23 ******** ******** PERCENT SEMI COMPOS ANNUAL REQUIREMENT / _) .. --------_. NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I C e rt i fy under pena l l y o f l a w tha t this d oc ument an d all a tt a c hm en ts were prepare d di r ection or s u p ervi s ion i n a cco r da n ce w ith a s yst em d esigne d to assure tha t qu a lifie d Anthony L. Williams p er s onn e l p ro p e r ly ga t her an d ev a lu at e th e in f or m ation submi tt ed. Based o n my i n qu i ry t he p e rs o n or p e r s o ns who ma n age t he system , or tho s e p erso ns d i r ectl y r es p o n s i b le f or g h a r ing the i nf o r matio n , the i nformation submi tt e d i s . to the best o f my knowledge and bel i ef , Site Vice President accurate , a nd complete. I am aware that there are significant pena l t i es for subm i tt i ng f alse i n f ormation, i nclud i ng the possibility of f i ne and i mpr i sonment for knowi n g v i olat io ns. T Y PED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLAT I ONS (Referen c e all attachments here) Toxicity was sampled April 30 -May 5 , 2017. EPA Form 3320-1 (REV 3/99) P r e vious edi t io ns m a y be used _/ --....,,__ 1te Vice President SIGNATURE OF PRINC I PA L E XECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE 423 843-7001 17 06 07 I A R EA I NUMBER YEAR MO DAY CO DE Pa!'.le 1 of 1 PERMITTEE NAME/ADDRESS (I n clu de Fac ili t y Name/L ocation if Different) Nam e _ ___ _ Addres.L_ 2000 ___________ _ ___ .J!.NTEROFFICE OPS-5 N-SQ.t:l.l _______ _ NAT I ONAL POLLUTANT DISCHARGE ELI M INA TIO N SYSTEM (N POES) D I SCHARGE MONITORING REPORT (DMR) TN0026450 103 G MAJOR (SUBR 0 1) F -FINAL Form App r o ved. OMB No. 2040-000 4 _ _ _ _§ODDY -DAISY .._lN_]7381_ _______ _ LOW VOL. WASTE TREATMENT POND Facl!l!Y _ _lYA -SEQUOYAH NUCLEAR PLANT _____ _ Locat i on_ _!jAMIL TO Ji.COUNTY..... _________ _ EFFLUENT ATIN: M illi cent Gar l and NO DISCHARGE D ... NOTE: Read ins truct ion s before completinQ th i s form. PARAMETER IX QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UN ITS MIN I MUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ******** ******** 7.2 ******** 7.5 0 5 / 31 GRAB MEASUREMENT .. 12 00400 1 0 PERMIT ******** ******** ** 6.0 ******** 9.0 SU ONCE/ GRAB EFFLUENT GROSS REQU I REMENT MINIMUM MAXIMUM WEEK SOLIDS , TOTAL SUSPENDED SAMPLE ******** ******** ******** 4.5 4.5 0 1 I 31 GRAB MEASUREMENT .. 19 00530 1 0 PERMIT ******** ******** .. ******** 30.0 100.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQU I REMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE ******** ******** ******** <5.0 <5.0 0 1 I 31 GRAB MEASUREMENT .. 19 00556 1 0 PERMIT ******** ******** .. ******** 15.0 20.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH FLOW , IN CONDUIT OR THRU SAMPLE 1.679 1.757 ******** ******** ******** 0 4131 INST AN 03 ** TREATMENT PLANT MEASUREMENT 50050 1 0 PERM I T Req. Mon. Req. Mon MGD ******** ******** ********* ** ONCE/ INST AN EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX WEEK SAMPLE MEASUREMENT PERMIT REQU I REMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERM I T REQUIREMENT --*--NAME/TITLE PRINCIPAL EXECUTIVE OFF ICER "'"' °' '"'" '" " '" -* p ,.,"."' TELEPHONE DA T E d irection or supervis i on in accordance w i th a system des i gned to assure that qual i f ied Anthony L. W il liams personne l p roperly gather and evalua t e the i nformat ion submi ll ed. Based on my i nqu i of th e person or perso ns who manage the system , or those persons directly responsible fo ather i ng th e i nformation , the information subm ille d i s , to the best of my knowledge and be li , true , 423 843-7001 17 06 07 Site V ic e President accurate , and comp le te. I am aware that there are s i gn i ficant penalt i es for submilli fa l se SIGNATURE OF PRINCIPAL EXECUTIVE I information, i n clu d i ng the poss i bili t y of fine and i mprisonment fo r knowing violations. O FF ICER OR AUTHORIZED AGENT ARE A I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY V I OLATIONS (Referen ce all attachments here) EPA Form 3320-1 (REV 3/99) PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if D ifferent) Name _ ___ _ Addres_L 20QQ... ___________ _ ___ .J.[NTEROFFICE OPS-5N-S(llil _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (N PDES} DISCHARGE MONITORING REPORT (DMR} TN0026450 110 G MAJOR (SUBR 01) F -FINAL Form Approved. OMB No. 2040-0004 _ _ _ _.§.ODDY -DAISY JN_1738L_ _______ _ Fac.illJY_ .JYA -SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ _!!AMIL TOJi..COUNTy_ _________ _ RECYCLED COOLING WATER EFFLUENT ATIN: Millicent Garland ... NO DISCHARGE I xx I ... NOTE: Read instructions before completini:i this form. PARAMETER x QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** .. CENTIGRADE MEASUREMENT 00010 1 0 PERMIT ******** ******** ** ******** ******** REPORT EFFLUENT GROSS VALUE REQUIREMENT DAILY MX TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** .. CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ******** ******** ** ******** ******** 30.5 INSTREAM MONITORING REQUIREMENT DAILY MX TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** .. UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT ******** ******** .. ******** ******** 5 EFFLUENT GROSS VALUE REQUIREMENT DAILYMX FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** ******** 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** EFFLUENT GROSS VALUE REQUIREMENT DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** .. MEASUREMENT 50060 1 0 PERMIT ******** ******** .. ******** 0.1 0.1 EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILYMX TEMPERATURE -C, RATE OF SAMPLE ******** ******** ******** ******** 04 CHANGE MEASUREMENT 82234 1 0 PERMIT ******** 2 DEGC ******** ******** ******** EFFLUENT GROSS VALUE REQUIREMENT DAILY MX SAMPLE MEASUREMENT PERMIT REQUIREMENT v----* --------/ --j---------- ---NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 'C"'fy ""'" """ * """" '"' '"" "' """""" direction or supervision i n accordance with a syslem designed to assure that qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inq u i ry the person or persons who manage lhe sys tem, or those persons directly responsible for g thering the i nformation , the i nformation submitted is , to the best of my knowledge and bel ie f , Ir Site Vice Pres i dent accurate , and complete. I am aware that there are significant penalties for submitting fa lse information , includin g the poss i bility of fine and imprisonment for know i ng violat i ons. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Per i od EPA Form 3320-1 (REV 3/99) Previous editions may be used / ice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous .. ** CONTIN RCORDR uous 19 MG/L Five per CALCTD Week .. .. CONTIN CALCTD uous TELEPHONE DATE 423 843-7001 17 06 07 I AREA I NUMBER YEAR MO DAY CODE Paqe 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ AddresL ___________ _ ___ _J!NTEROFFICE OPS-5N-SQN) _______ _ _ _ _ _.§.ODDY -DAISY JN_j7384_ _______ _ Faclfily_ ...IVA -_fil:QUOYAH NUCLEAR PLANT _____ _ Location_ _!:!AMIL TOJiCOUNTL ________ _ ATIN: Millicent Garland NATI O NAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER 1 1 MAJOR (SUBR 01) F *FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I xx I ... Form Approved. OMB No. 20 4 0-000 4 From I : To Hf I °tf NOTE: Read instructions before completinQ this form. PARAMETER I X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MIN I MUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** ** 23 CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERMIT ******** ******** **** 42.8 ******** **"'***** PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** .. 23 PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMIT ******** ******** **** 42.8 ******** ******** PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT ../,...... -/ NAMErrlTLE PRINCIPAL EXECUTIVE OFFICER /__ TELEPHONE DATE ', .. ""'" .. '"" '"" ""-'"' ., """'-" . .,. .. '¥ d i rect i on or super vi s i on i n accordance with a s y stem d e s i gn e d lo assur e Iha! qua li f i ed Anthony L. Williams personne l prop e rly gath e r and evaluate !he inform a ti o n submilled. Based on my i nqu i ry f !he ------pe r son or p e rs o ns who man ag e !h e syst e m , or !hose p er s o n s d i r ec tly r e sp o n s i ble f o r g lh e r i n g Site V i ce President !h e in f o r mation , !h e i nformat i o n subm ill ed i s . lo !he bes! of m y k n owl ed ge a nd be lief , l r u ac c u r ate , a n d com ple te. I am a wa r e I ha! !he re a r e si g nifica n t pe n a lt i e s f o r s ub mi ll i n g f al s e i nform a t i on , i n cl ud i ng th e p o s si b ili ty of f i ne and i mp ri s on m e nt f o r k n ow i ng vio lat i on s. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous edit i on s may be use d Site Vice President SIGNATURE OF PRINCIPAL EXECU TI VE OFFICER OR AUTHORIZED AGENT 423 843-7001 17 06 07 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1 PERM I TTEE NAME/ADDRESS (I nc lu de Fac i l ity Name/Locat ion if D i ffe rent) Name _ ___ _ Add r es_L 2000 ___________ _ ___ _ilNTEROFF I CE OPS-5N-SQNl _______ _ _ _ _ __§.ODD..Y_

• DA I SY

_______ _ FacJilly _ _.IVA-_fil:QUOYAH NUCLEAR PLANT _____ _ Locat i o..Q_ .J:!AMIL TOli_COUNTL _________ _ ATTN: Millicent Gar l and NA TI ONAL POL L U T A N T D I SC HA RGE ELI M I N A T I ON SYST E M (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 PERMIT NUMBER 118 G DISCHARGE NUMBER DAY 31 MAJOR (SUBR 0 1) F-FINAL WASTEWATER & STORM WATER EFFLUENT ... NO DISCHARGE I xx I *** Fo r m App ro ved. OMB N o. 20 4 0-000 4 NOTE: Read instructions before complet i nQ t h i s form. PARAMETER I X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUEN C Y SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS M I NIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMP L E ******** ******** ******** ******** MEASUREMENT .. 19 00300 1 0 PERMIT ******** ******** **** 2 ******** ******** MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** ******** .. 19 MEASUREMENT 00530 1 0 PERMIT ******** ******** ***" ******** ******** 100 MG/L TWICE/ GRAB EFFLUENT GROSS REQU I REMENT DAILY MX WEEK SOLIDS , SETTLEABLE SAMPLE ******** ******** ******** ******** .. 25 MEASUREMENT 00545 1 0 PERMIT ******** ******** .... ******** ******** 1 ML/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** 03 .. TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** ******** ********

• ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MO AVG DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

...----. / --NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Ce rt i fy und e r p enal t y of law th a t th is d ocu m en t an d a ll a tta c hm ents w ere p re p a red un de r TE L EPHONE DATE d irecti on or su p ervi s i on in accor d a n ce w i th a system d e s ign ed to as s u re t h at q u al i fied Anthony L. W i lliams personnel prope r ly gat h er an d evalu a te the i n forma t ion submi tt ed. B ased on m y i n qui of t h e -perso n or perso ns w ho ma nage t h e sys t em , o r t h ose pe r so n s d i r ec t ly respons i ble f ot gathe r i n g t he informat i on , the i nformation subm i tted i s . lo the best of my knowledge a n d belie true , 4 2 3 843-7 00 1 17 06 07 Site V i ce Pres i dent accurate , and comple t e. I am aware that there are s i gnificant pena l t i es for subm i tt in l se i--s1 G NATURE OF PR I NC I PAL EXE CUT IV E I information , including t h e possibi l ity o f fine a n d impr i sonmen t fo r know i ng violations. -OFFICER O R AUTHORIZED AGENT AR E A I NUMBER Y E AR MO DAY TYPED OR PR I NTED CO DE C OMMENTS AND EXPLANAT I ON OF ANY VIOLATIONS (Refer e nce a ll a ttachments here) Dur i ng th i s r eport i ng pe ri od , there has been n o flow from t he Dredge Pond other than that resu lti ng from ra i nfa ll. No Discha rg e th i s Per i od EPA Form 3320-1 (REV 3/99) Previous editions may be used Paae 1 of 1 TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: May 24, 2017 1. Facility I Discharger

Sequoyah Nuclear Plant I TVA 2. County I State: Hamilton I Tennessee

3. NPDES Permit#: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant 5. Design Flow (MGD): 1,579 6. Receiving Stream: Tennessee River CTRM 483.6) 7. lQlO: 3,491 8. Outfall Tested: 101 9. Dates Sampled: April 30 -May 05, 2017 10. Average Flow on Days Sampled (MGD): 1834. 1834. 1817 11. Pertinent Site Conditions:

Production I operation data will be provided upon request. 12. Test Dates: May 02 -09, 2017 13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia) 15. Concentrations Tested(%): Pimephales promelas: UV treated Outfall 101: 10.7. 21.4, 42.8. 85.6. 100 UV treated Intake: 1 00 Ceriodaphnia dubia: Non-treated Outfall 101: 10.7. 21.4. 42.8. 85.6. 100 Non-treated Intake: I 00 16. Permit Limit Endpoint(%): Outfall ] 01: IC2.2. = 42.8% 17. Test Results: Outfall 101: Pimephales promelas: IC25. > 100% Ceriodaphnia dubia: lC.25. > 100% Page 1of94

18. Facility Contact: Millicent Garland Phone#: (423) 843-6714 19. Consulting I Testing Lab: Environmental Testing Solutions, Inc. 20. Lab Contact: Jim Sumner Phone#: (828) 350-9364 21. TVA Contact: Donald W. Snodgrass Phone#: (256) 386-2787 22. Notes: Exposures to samples collected April 30 -May 05, 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids.

The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Page 2of94 METHODS

SUMMARY

Samples: 1. Sampling Point: Outfall l 01, Intake 2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (o C) (mg/L) Last Used Bv 101 04-30-17 0805 to 05-01-17 1332 4.l , 2.2 t <0.10 05-02-17 0920 05-01-17 0705 05-03-17 0846 Intake 04-30-17 0710 to 05-01-17 1332 1.7 <0.10 05-02-17 0920 05-01-17 0610 05-03-17 0846 101 05-02-17 0705 to 05-03-17 1235 2.1 , l.7 t <0.10 05-04-17 0850 05-03-17 0605 05-05-17 0852 Intake 05-02-17 0730 to 05-03-17 1235 2.2 <0.10 05-04-17 0850 05-03-17 0630 05-05-17 0852 05-04-17 0730 to 05-06-17 0930 101 05-05-17 1230 1.1 , l.3 t <0.10 05-07-17 0952 05-05-17 0630 05-08-17 0833 05-04-17 0745 to 05-06-17 0930 Intake 05-05-17 1230 0.9 <0.10 05-07-17 0952 . 05-05-17 0645 05-08-17 0833 *TRC = Total Residual Chlorine t samples were c o llected in two 2.5 gallon cub i tainers. Temperature was measured in each cubitainer upon arrival. 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- l .0°C) in a warm water bath. Page 3of94 Aliquots of Outfall l 0 l and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Pimephales promelas Test Organisms:

1. Source: In-house Cultures 2. Age: < 24-hours old Test Method Summary: l. Test Conditions:

Static, Renewal 2. Test Duration:

3. Control I Dilution Water: Moderately Hard Synthetic

4. Number of Replicates

1 5. Organisms per Replicate:

lQ 6. Test Initiation: (Date/Time): 05-02-17 0832 ET 7. Test Termination: (Date/Time): 05-09-17 0857 ET 8. Test Temperature: Outfall 101: Mean= 24.7°C (24.2 -25 .1°C) 9. Physical I Chemical Ce riodaphnia dubia In-house Cultures < 24-hours old Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic l 05-02-17 0920 ET 05-09-17 0827 ET Mean = 24.9°C (24.6 -25.2°C) Measurements

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each I 00% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and exposure test solutions were analyzed daily for pH and dissolved oxygen. 10. Statistics:

Page 4of94 Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA). TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 02 -09, 2017 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used -days) (% Effluent) 1 2 3 4 5 6 7 Control , 100 100 100 100 100 100 100 UV-treated 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Control , 100 100 100 100 100 100 98 Non-treated Test Solutions Mean Dry Weight (mg) (replicate number) (%Effluent) 1 2 3 4 Mean Control , 0.632 0.723 0.732 0.703 0.698 UV-treated 10.7% 0.700 0.704 0.701 0.677 0.696 21.4% 0.730 0.701 0.689 0.771 0.723 42.8% 0.687 0.642 0.662 0.684 0.669 85.6% 0.633 0.719 0.657 0.666 0.669 100.0% 0.644 0.649 0.627 0.637 0.639 Intake 0.650 0.605 0.596 0.688 0.635 Control , 0.632 0.783 0.630 0.669 0.679 Non-treated IC 2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LC so: TUc = 100/ IC 2s Page 5of94 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a C eriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 02 -09, 2017 using effluent from Outfall 101. Percent Surviving Test (time interval used -days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 . JOO Test Solutions Reproduction (#young/female/7 days) Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 33 28 30 32 35 30 32 30 32 31.2 10.7% 34 36 31 34 33 31 32 35 31 31 32.8 21.4% 37 36 31 33 32 34 31 33 32 37 33.6 42.8% 35 36 37 32 33 33 35 34 36 33 34.4 85.6% 37 35 34 38 32 38 38 36 39 32 35.9 100.0% 38 36 37 36 37 39 39 35 38 33 36.8 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LC 5 o: TUc = 100/ IC 2s Page 6of94 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 02 -09, 2017 using water from Intake Percent Surviving Test (time interval used -da y s) Solutions 1 2 3 4 5 6 7 (% Effiuent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effiuent) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 33 31 32 34 34 31 28 32 30 31 31.6 Intake 38 34 36 37 35 31 37 32 34 32 34.6 IC2 s Value: > I 00% Calculated T U Estimates: < 1.0 TUc* Permit L imit: N I A Permit Limit: N I A 95% Confidence Limits: U pper Limit: NA L ower Limit: NA *TUa = 100/LC 5 o: TUc = 100/ IC 2 5 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Spec i es Date Time Duration Toxicant Results (IC 2s) Pimephales promelas May 02 -09 , 2017 0820 7-days KCI 0.67 g/L Ceriodaphnia dubia May 02 -09 , 2017 0911 7-days NaCl 1.07 g/L Page 7 of94 PBYSICALJCHEMICAL

SUMMARY

CQ Water Chemistry Mean Values and Ranges fi>r UV-treated Pimephales promelas and Non-treated Ceriodaphnia dubia , Sequoyah Nuclear Plant (SQN), Efiluent Outfiill 10 l and Intake CD perfi>nned May 02-09 , 2017. ClO 0 .... Test Sample ID Temperature ("C) Dissolved Oxn:en (llll?fL) Initial Final Initial Final Control, 24.7 24.5 7.8 7.0 Non-treated 24.6 -24.8 24.3 -24.8 7.7 -7.9 6.0 -7.8 Control, 24.7 24.4 8.0 7.1 UV-treated 24.6 -24.8 24.2 -24.7 7.9 -8.2 6.2 -7.7 10.7% 24.8 24.6 8.0 7.1 24.7 -24.9 24.4 -24.9 7.9 -8.1 6.2 -7.7 i: 21.4% 24.9 24.5 8.0 7.0 <>.. 24.7 -25.0 24.3 -24.7 7.9 -8.2 6.1 -7.7 "' 24.9 24.6 8.0 6.9 "' 42.8% --= 24.8 -25.0 24.2 -24.9 7.9 -8.1 6.0 -7.7 85.6% 25.0 24.6 8.0 6.9 Q., 24.9 -25.1 24.2 -24.9 7.9 -8.1 6.0 -7.7 100% 25.0 24.6 8.0 6.9 24.9 -25.1 24.2 -24.8 7.9 -8.1 5.9 -7.7 Intake 25.0 24.6 8.0 7.0 24.9 -25.l 24.4 -24.9 7.9 -8.1 5.9 -7.7 Control, 24.7 25.0 7.8 7.9 Non-treated 24.6 -24.8 24.9 -25.2 7.7 -7.9 7.8 -8.0 10.7% 24.8 25.0 7.8 7.9 24.7 -24.9 24.7 -25.2 7.8 -7.9 7.7 -8.0 .s 24.8 25.0 7.8 7.9 ..,, 21.4% .§ 24.7 -24.9 24.8 -25.2 7.8 -8.0 7.8 -8.0 .s 24.8 24.9 7.9 7.9 =: 42.8% --= .§' 24.7 -24.9 24.8 -25.0 7.8 -8.0 7.8 -8.0 85.6% 24.9 24.9 7.9 8.0 24.7 -25.0 24.8 -25.1 7.8 -8.0 7.9 -8.1 100% 24.9 24.9 8.0 8.0 24.7 -25.0 24.8 -25.2 7.9 -8.0 7.9 -8.2 Intake 24.9 24.9 8.0 8.0 24.7 -25.0 24.8 -25.0 7.9 -8.1 7.8 -8.2 *Note: Total residual chlorine was perfonned on non-treated Outfall 101 and Intake samples. Overall temperature ("C) Pimephales prome/as Ceriodaphnia dubia Average 24.7 24.9 Minimum 24.2 24.6 Maximum 25.l 25.2 Initial Final 8.13 7.69 8.08 -8.21 7.49 -7.92 8.09 7.72 8.05 -8.19 7.55 -7.93 8.11 7.67 8.06 -8.18 7.49 -7.89 8.11 7.67 8.04 -8.17 7.48 -7.87 8.09 7.67 7.98 -8.18 7.49 -7.87 8.09 7.67 7.99 -8.19 7.49 -7.86 8.08 7.65 8.03 -8.19 7.48 -7.87 8.09 7.69 8.03 -8.18 7.48 -7.91 8.13 8.03 8.08 -8.21 7.97 -8.09 8.12 8.02 8.06 -8.18 7.97 -8.09 8.13 8.01 8.09 -8.19 7.97 -8.08 8.12 8.02 8.08 -8.19 7.97 -8.09 8.12 8.02 8.08 -8.19 7.97 -8.10 8.08 8.01 7.95 -8.19 7.92 -8.09 8.09 8.04 8.02 -8.18 7.98 -8.11 Conductance Alkalinity Hanlness *Total Residual (µmhos/cm) (mg/L CaC03) (mg/L CaC03) Chlorine (mg/L) 322 62 85 -317 -328 61 -63 82 -320 62 87 -312 -326 61 -64 86 -307 ---295 -318 ------294 ---283 -305 ------259 ---250 -267 ------190 ---186 -193 ------165 64 65 <0.10 160 -169 59 -69 64 -66 <0.10 -<0.10 165 64 67 <0.10 158 -170 61 -67 64 -70 <0.10 -<0.10 322 62 85 -317 -328 61 -63 82 -308 ---298 -316 ------292 ---286 -300 ------258 ---248 -265 ------188 ---182 -196 ------164 63 65 <0.10 158 -171 59 -67 62 -66 <0.10 -< 0.10 166 64 66 <0.10 162 -177 59 -67 64 -68 <0.10 -<0.10

SUMMARY

I CONCLUSIONS Exposures to samples collected April 30-May 05 , 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values , for both species , were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Page 9of94 Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms , per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates , each containing one test organism, per treatment.

Test vessels consisted of 30-mL polypropylene cups , each containing 15-mL of test solution. DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL 1. Pimephales promelas Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction oftest organisms. UV treatment is used to control interference of fish pathogens. This treatment method was approved on November 23 , 2015 by the State of Tennessee in a letter from Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring. 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS I. Pimephales promelas None 2. Ceriodaphnia dubia None Page 10of94 PHYSICAL AND CHEMICAL METHODS I. Reagents , Titrants , Buffers , etc.: All chemicals were certified products used before expiration dates (where applicable). 2. Instruments: All identification, service , and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B-2010. 4. Dissolved oxygen was measured by SM 4500-0 G-20 I 1. 5. The pH was measured by SM 4500-H+ B-2011. 6. Conductance was measured by SM 2510 B-2011. 7. Alkalinity was measured by SM 2320 B-2011. 8. Total hardness was measured by SM 2340 C-2011. 9. Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including , but not limited to , sample collection , handling and storage , glassware preparation , test organism culturing/acquisition and acclimation , test organism handling during test , and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC2 s values in g/L KCI or NaCl. 2. Standard Toxicant:

Potassium Chloride (KC! crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia. 3. Dilution Water Used: Moderately hard synthetic water. 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses. Page 11of94 REFERENCES

l. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms , EPA-82l-R-02-013 (October 2002). 3. Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012. 4. Quality Assurance Program: Standard Operating Procedures , Environmental Testing Solutions , Inc (most current version).

Page 12of94 Page 13of94 Sequoyah Nuclear Plant Biomonitoring May 02 -09, 2017 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine , Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks , During Toxicity Test Sampling , February 6, 2005 -May 5 , 2017 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L TRC EO/PO 02/06/2005 -<0.0042 0.028 0.010 ----02/07/2005 -<0.0116 0.028 0.010 ---0.007 02/08/2005 -<0.0080 0.028 0.010 ----02/09/2005 -0.0199 0.028 0.010 ----02/10/2005 -<0.0042 0.028 0.010 ----02/1112005 -0.0155 0.028 0.010 ---0.007 06 1 05 1 2005 -0.0063 ------06 1 06 1 2005 -0.0043 ------06 1 07 1 2005 -0.0103 ------06/08/2005 -0.0295 ------06 1 09 1 2005 -0.0129 ------06/10/2005 -0.0184 ------07117/2005 -0.0109 0.026 0.009 ----07/18/2005 -0.0150 0.026 0.009 ----07 1 19 1 2005 -0.0163 0.026 0.009 ----07/20/2005 -0.0209 0.026 0.009 ---0.014 07/21/2005 -0.0242 0.026 0.009 ----07/22/2005 -0.0238 0.054 0.018 ---0.014 I 0/30/2005 -0.0068 ------10/3112005 -0.0112 ------11/01/2005 -0.0104 ------11/02/2005 -0.0104 ------11/03/2005 -0.0117 ------11/04/2005 -0.0165 ------11/14/2005 -0.0274 ------11115/2005 -0.0256 ------11/16/2005 -0.0234 ------11117/2005 -0.0231 ------11/18/2005 -0.0200 ------11119/2005 -0.0116 ------Page 14of94 H-150M mg/L Quat -------0.037 0.037 0.037 ---0.036 0.036 0.036 ----0.035 0.036 0.036 0.035 ------ Date 11/12/2006 11/13/2006 11/14/2006 11/15/2006 11/16/2006 11/17/2006 11/26/2006 11/27/2006 11/28/2006 11129/2006 11/30/2006 12/01/2006 05/28/07 05 1 29 1 07 05/30/07 05/31/07 06 1 01 1 07 06 1 02 1 07 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04/15/08 04/16/08 04117 1 08 04/18/08 I 0/26/08 10/27/08 10/28/08 10/29/08 I 0/30/08 10/31/08 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks , During Toxicity Test Sampling , February 6, 2005 -May 5 , 2017 Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L TRC EO/PO -0.0055 -------0.0068 -------0.0143 -------0.0068 -------0.0267 -------0.0222 -------0.0188 -------0.0138 -------0.0120 -------0.0288 -------0.0376 -------0.0187 -----------------------0.0084 -----0.017 -0.0103 -------0.0164 -----0.017 -0.0305 -------0.0241 -------0.0128 -------0.0238 -------0.0158 -------0.0162 -------0.0175 -------0.0039 -------0.0124 -------0.0229 -------0.0143 -------0.0120 -------0.0149 -------0.0260 -------0.0151 -----0.017 -0.0172 -------0.0154 -----0.018 ---------0.0086 ------Page 15 of 94 H-150M MSW mg/L 101 Quat mg/L Phosphate --0.037 -0.037 -0.037 -0.037 ----------------0.015 0.036 0.015 0.036 0.015 0.036 0.015 0.036 0.015 -0.015 ---------------------------0.041 -0.041 0.030 0.041 0.030 0.041 0.030 Date 0 2/08/09 0 2/09/09 0 2/1 0/09 02/1 1/09 02 11 2/09 02/1 3/09 05 1 10 1 09 05 1111 09 05/1 2/09 05 11 3 1 09 05 11 4 1 09 05 11 5 1 09 11 115 1 09 11 1 16 1 09 11/1 7/09 11 /1 8/09 11/1 9/09 1 112 0 1 09 05 1 09 11 0 0 5/10/10 0 511 1 11 0 05/1 2/1 0 05/1 3/1 0 05/14/1 0 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Micro biologically Induced Corrosion and Mollusks , During Toxicity Test Sampling , February 6 , 2005 -May 5 , 2017 Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus Hypochlorite mg/L 222 mg/L mg/L -PF m g/L mg/L 73551 CT1300 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg!L TRC Phosph EO/PO Quat ate -0.0197 -----0.017 --0.0237 -----0.017 --0.0104 -----0.021 --0.0155 -----0.017 --0.0106 -----0.017 -----------0.0129 --------0.0415 --------0.0053 --------0.0049 --------<0.0141 --------<0.0160 --------0.025 --------0.0152 --------0.0255 --------0.0306 --------0.0204 -------0.0093 --------0.0192 --------0.0055 --------0.0100 ------0.039 -0.0171 ------0.039 -0.0041 -----0.039 -0.0099 ------0.039 Page 16of94 H-150M MSW mg/L 101 Quat mg!L Phosphate

---

0.0446 -0.0396 -0.0396 -0.0397 ---------------------------

D a t e 10/31/IO I I /0 1/1 0 1 1/02/10 11/03/1 0 I 1/0 411 0 1 1/0 5/10 0 5/01/2 011 0 5/0 2/2 011 0 5 1 0 3 1 2 0 I I 0 5/0 4/2 011 0 5 1 0 5 1 2 011 0 5/0 6/2 011 l l/0 6/2 01 I 11/0 7/2 011 11/0 8/2 011 11/0 9/2 011 1 1/]0/2 011 11/J 1/2 011 0 5/0 6/2 01 2 0 5/0 7/2 01 2 0 5/08/2 01 2 0 5/0 9/2 01 2 0 5/10/2 01 2 0 5/J 1/2 01 2 08/J 2/2 01 2 08/13/2 01 2 08/14/2 01 2 08/J 5/2 01 2 08/J 6/2 01 2 08 11 7/2 01 2 05/12/2 0 13 0 5113 1 2 01 3 0 5/1 4/2 01 3 0 5115/2 01 3 0 5/1 6/2 01 3 0 5/17/2 01 3 09/1 5/2 01 3 09 11 6/2 0 I 3 0 9/1 7/2 01 3 0 9/1 8/2 01 3 0 9/19/2 01 3 0 9/2 0/2 01 3 Table B-1. Sequoyah Nuclear Plant Outfall l 01 Diffuser Dis c harge Concentrations of Chemicals Used to Control Microbiologicall y Induced Corro s ion and Mollusks , During To x icity Test Sampling , Februa ry 6 , 2005 -Ma y 5 , 2 017 Sodium Towerbr o m PCL-P C L-401 CL-363 C u pro s tat H-130M N alco Spectrus H-150M Hypo-m g/L 222 m g/L mg/L -PF m g/L mg/L 73551 CT1300 mg/L chlorite TR C mg/L C opoiy-DMAD A zoi e Quat m g/L mg/L Quat mg/L Phos-mer E O/PO Quat TRC oh ate -----------0.01 22 ---------0.011 2 ---------0.01 63 ---------0.010 7 ---------0.01 32 --------------------------0.04 ---------0.04 --0.0 155 ------0.04 --0.017 9 ------0.04 --0.008 9 ---------0.0168 ---------0.0 225 ---------0.01 41 ---------0.0 239 ---------0.0 242 ---------0.0 231 ------------------------------------0.041 --0.0 145 ------0.041 --0.0 298 ------0.041 --0.017 4 -------------------0.0 256 -----0.0 2 8 0.037 --0.0 209 ------0.037 --0.0 2 7 9 -----0.02 8 ---0.007 6 ---------0.0 446 ---------0.00 99 ------------------0.00 91 -----0.0 3 9 ---0.00 96 -----0.03 9 ---0.0 229 ---------0.00 63 -------------------0.00 72 ------0.0379 --0.0 10 7 -----0.0 3 6 0.0379 --0.0 217 -----0.0 3 6 0.0379 --0.0 172 ---------0.01 7 3 --------Page 17of94 MSW F ioguard 101 M S6 23 6 mg/L m g/L Phos-P h os phat e oh ate -------------------------------------------------0.0 29 -0.0 2 9 -0.0 2 9 -0.0 2 9 -0.0 2 9 -0.0 32 ---0.064 -0.06 4 -0.064 -0.0 32 -0.0 32 -0.03 -0.0 3 -0.0 3 -0.0 3 -0.0 3 -0.0 3 Date 05/04/2014 05/05/20 1 4 05/06/2014 05/07/2014 05/08/20 14 05/09/20 14 09/07/20 1 4 09/08/2014 09/09/2014 09 11 0/2014 09/11/2 014 09112 1 2 01 4 08/09/20 1 5 08/J 0/2015 08/1 1/2015 08/1 2/20 1 5 08/13/2 01 5 08/\ 4/20 1 5 I 0/18/2 015 I 0/1 9/20 1 5 10/2 0/2015 10/2 1/2 01 5 10/22/2 01 5 10/23/20 1 5 05 11 5/2016 05 11 6 1 20 1 6 05/1 7/20 1 6 05/18/2 016 05/1 9/2016 05/20/2016 0 7131/2016 08/0 1/20 16 08/02/20 16 08/03/2 01 6 08/04/20 16 08/05/20 1 6 04/30/20 1 7 05/01/20 1 7 05/02/20 17 05/03/2 017 05/04/20 17 05/05/20 17 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Co ncentrations of Che micals Use d to Control Microbiologically In duced Corrosion and Mollusks , Durin g Toxic ity Test Sampling, February 6 , 2005 -May 5 , 201 7 Sodium Towerbrom PCL-P CL-401 CL-363 C u pro stat H-130M Nalco Spectrus H-150M Hypo-mg/L 222 mg/L mg/L -PF m g/L mg/L 73551 CTl300 mg/L chlorite TRC mg/L Co poly-DMAD Azo l e Quat m g/L mg/L Quat mg/L Phos-mer EO/PO Quat TRC phate -0.0118 ---------0.0112 ---------0.0096 ---------0.0164 ---------0.0235 ---------0.0110 --------------------------0.04 ---------0.04 ---------0.04 --0.007 0 ---------0.0074 ----------0.0195 0.03 0.0275 0.03 0.0213 -0.0192 -0.0182 -0.0162 0.0125 0.0120 0.0130 0.0174 0.0156 -0.0 2 09 0.0210 0.0361 0.0254 0.0261 --0.0091 0.03 0.0093 0.03 0.0209 0.03 -----0.0298 0.0218 0.0260 --Page 18of94 MSW Floguard 101 MS6236 mg/L mg/L Phos-Phosphate phate ---------------------------0.03 0.03 0.0 3 Page 19of94 Sequoyah Nuclear Plant Biomonitoring May 02 -09, 2017 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets BIOMONITOIUNG CHAIN O F CUSTODY RECORD TV A Environmental Testing Solution, Inc. Delivered By (Circ l e O ne): I Q) FedEx UPS Bus Client 'oject Name: Sequoyah NP Toxicity 3 51 Depot Street. l'1 Asheville , NC SoV'\I Q_ b (:/,'verj Number: N/ A Other (specify): 0 Sampled: Sequoyah NP 28801 General Comments: "/00,e /)0 1-> /.JJ/ "7.50 m-. 5-/-/? NPDES Number: TN0026450 Phone: 828-350-9364 Too.t !Aflue_e>7.Jot?;.S-l

• f/ Fax: 828-350-9368 Collected By 0 1' o;.s.s. Jf'?-e!J µe.,,re C!Pll-edeJ ,t-_kej>I /IL v \ I i)l\.DJ I 23az. Con t ainer Field Identific a tion I Number& Flow R ain Eve n t? Sample De sc ription Grab/C omp. Collec tion Datetrime Volume (MGD) Laboratory Use Collected (Mark as Appropriate)

.* --" *)*:O* ---\""" "(\, : , -:.. If Yes , ETS Log Arrival T e mp. By Time A pp ear* ;*,* .L .. .. ..... : * ,., : S t art E nd "*-* Yes No Trace N umber ("C} ... ",' __ .. .,,..-{.;,/ -;. ' -In che s E\ an cc 'l-30-/7 5-/-/7 1 Y33.q;J. x 0.51 c.l.\ \ '2..1.:L \?]:>'-SQN-101-TOX Comp ()'7C>S-.Ex 2(2.Sgal) -A SQN-INT-TOX Comp l/*JD-17 s-/-17 1(2.5 gal) A/14 x o.51 \.1*c_ \g,1..-.f. Ob/D Sample Custody-Fill In From Top Down -is (' .i<"t"Ot:$4 C"o-"\.< Relinquished By (Signature)

Date/Time Received By (Signature)

1tl <l-OOb A\l... S".-\P\tS lnL vd //y//l 5=!-17 SOt..l\(.. tJS---0/-1? / o<e.*) *£T (....-v / -tSI /3.3\.. I / Bl( 1-tr"J (' 1"\ Er "---" Instructions

Clients should fill in all areas except those in the "Laboratory Use'* block. Biomonitoring samp l es are preserved by storing them al 6°C and shipping them in ice. The hold time for each sample is 36 hou r s from the time of collection.

Therefore. please collecl and ship in such a way that the laboratory wil receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive un t il the following Monday. Whole Effluent Samp l e Receipt Log Page 52 *s ample temperature perf orm ed using Sample Rece i vitlll Thermometer

SN 160928622 Date T i me Received Received *sample Project Sample Sample name and description State Comments Received Received by from Temp. (0 C) number number 05-01-17 1245 J. Sumner l. Keenan 4.1 12330 170501 .01 Jefferson WWTP NC I 05-01-17 1245 I. Su m.,er l. Keenan 4.1 12331 170501 .02 West J e fferson WWTP NC I 05-01-17 1332 J. Sumner TV A Courier 4.1/2.2 12332 170501 .03 TVA -Sequoyah Nuclear Plant-101 TN I 05-01-17 1332 J. Sumner TVA Courier 1.7 12332 170501 .04 TVA -Sequo yah Nuclear Plant -Intak e TN SOP G4 -Exhib i t G4.2 , revision 01-03-12 BIOMONITORING CHAIN OF C U STODY RECORD Page _l_ of _1_ 'tjent: TVA Env ironmental Testing Solution, Inc. Delivered By (Ci rcl e One): I _.., FedEx UPS Bus Client 'fioject Name: Sequoyah NP Toxic ity 351 Depot Street. Number: N/ A Asheville, NC Other (specify):

52 . ...., ,*c_ Del<<uer'y &ility Sampled: Sequoyah NP 28801 General Comments (' 'bv,,<< It:!_ (!! P7,y; on S: J'-1-; NPDES Number: TN0026450 Phone: 828-350-9364 1(;0.e:_ lro,...,_1.,..f!. -/P<>/U-S tJ>_,.,,,,,, "--f.(""'1 Fax: 828-350-9368 Collected -x O c'15 .riM-r AX'C._ Conta in er f fUJj l 2 3 3 '2. F ield I dentification I Number& Flow Ra in Eve nt? S ample De scrip tion Grab/Comp. Collection Dateffime Volume (MOD) Laboratory Use Collected (Mark as Appropriate) r<"' . ., . . .. 11;__;/_ . * . -* -. .,-;.. *i . If Yes. ETS Log Arrival Temp . B y T im e Appear-*.: ___ ,: ... ... :-r? ),

• Sta n End . f ** _: :.1'!'l* 't ,: ', 7 ? .. Yes Inches No T ra ce Nu mbe r (°C) Ao .. , ... 'le...:' l"':.!-an cc S-3-17 13'33.?i I -i. \ \ \,\ *c_

1t SQN-101-TOX Comp IJ'705Ei otoDS-e.' 2(2.5gal) 5-2-1 7 S-3-/7 1(2.5 gal) NA x t..1.: (.. ""';S >rr-SQN-INT-TOX Comp 07:?0 *1 t>IP3DE' Sample Custody-Fill in From T op Down 1..n-11(...Ji"". Relinqui shed B y (Signature). Date/Time Recei ved By (Signature)

6-ion

\l\L ate/Time ii>...} A\...'. --I .5-3-11/ot1& /3 R SON\(_ O§vcJJ .... /1 '. ... '--.;-_ . d\ o<i/'C E\ & v / , ) J'r_ 12:) f' E.:'" r '1Mt. / o.s-<r'::>-I\ \l._)5 E\ v . I ns tru cti on s: C li e nt s s h ou ld fill in a ll a re as exc ept tho se in the *'L ab o ra to ry Use** b l o c k. B i o m o n it ori n g sa mples a r c pr ese r ve d by s torin g t hem at 6°C and s hippin g th em in i ce. TI 1 e h old t ime fo r each sa m ple is 36 hou r s from the t i me o f co ll e cti o n. Therefo r e. please collect a nd s h ip in *u c h a w ay t hat t h e l abor atory w ill recei v e th e sa m ples w it h ample l i me t o i nitiate t esting w ithin t hat time frame. Samples shipped overn i ght on Friday v ia FedEx or U P S mus r. be marked fo r Satu r day de l ive r y or they will not arrive u nt i l t h e fo ll ow i ng Monday. Whole Effluent Sample Receipt Log

• samp l e temperatu r e performed us i ng Sample Rece i ving Thermom e ter: SN 1 60928622 Date Time Received Received *sample Project Samp l e Sample name and description State Comments Received Received by from Temp. (0 C) number numb e r 05-03-17 0910 K.Keenan Fed -Ex 0.7 12346 170503 .01 Rockingham WWTP NC 05-03-17 0950 K.Keenan Fed -Ex 1.0 12347 1 7 0503 .02 ApexWRF NC 05-03-17 0950 K.Keenan Fed -Ex 1.2 12348 170503 .03 Belews Creek SS NC 05-03-17 0950 K.Keenan Fed* Ex 4.1 12349 170503 .04 Da i kin Appl i ed Americas NC 05-03-17 0950 K.Keenan Fed -E x 0.3 12350 170503 .O S Dan River CC NC 05-03-17 0950 K.Keenan Fed -Ex 2.4 12351 1 70503 .06 Dav i dson Water WTP NC 05-03-17 0950 K.Keenan Fed* Ex 0.8 12352 170503 .0 7 Dobson WTP NC 05-03-17 0950 K.Keenan Fed* Ex 1.2 12353 170503 .08 James Loughl i n WWTP NC 05-03-17 0950 K.Keenan Fed* Ex 0.8 12354 170503 .09 Performance Fibers NC 05-03-17 0950 K. Keenan Fed* Ex 1.2 12355 170503 .1 0 Roseboro WWTP NC 05-03-17 0950 K.Keenan Fed -Ex 0.3 12356 170503 . I I Scarlett Acres MHP NC 05-03-17 0950 K. Ke enan fed -Ex 0.8 12357 170503 .12 Sparks Road WTP NC 05-03-17 0950 K. Keenan Fed* Ex 2.0 12358 170503 .13 E l ementis NC 05-03-1/ 0948 K.Keenan UPS 1 0.5 1 2359 170503 .1 4 Morehead City WWTP NC E x ceede:J Temp. requ i rement 05-03-17 0950 K. Keenan Fed -Ex 1.7 12360 170503 .15 AQWA NC 05-03*17 0950 K. Keenan Fed -Ex 1.7 12361 170503 .16 Bladen B l uffs WTP NC 05-03-17 0950 K.Keenan Fed -Ex 1.7 12362 170503 .17 Buckeye Termina l s NC 05-03-17 0950 K.Keenan Fed-Ex 1.2 12363 170503 .18 lnvisita
• 001 NC 05-03-17 0950 K. Keenan Fed -Ex 1.2 12364 170503 .19 lnvisita
• 002 NC 05-03-17 0950 K. Keenan Fed* Ex 0.1 12365 170503 .20 Man t eoWWTP NC 05-03-17 0950 K. Keenan Fed -Ex 0.3 12366 170503 .21 Mayo Stream Electric P l ant NC 05-03-17 0950 K. Keenan Fed -Ex 1.2 12367 170503 .22 Pender County WTP NC 05-0 3-17 0950 K.Keenan Fed* Ex 1.3 12368 170503 .2 3 Shearon Harris Plant NC 05*03-17 0950 K.Keenan Fed -Ex l.5 12369 170503 .24 Shearon Harris E&E Center NC 05-03-17 095Q K.Keenan Fed -Ex 1.0 12370 170503 .2 5 Washington WWTP NC 05-03-17 0950 K.Keenan Fed* Ex 1.2 1237 1 170503 .26 PCS Phosphate, Inc. NC 05-03-17 0950 K.Keenan Fed* Ex 0.3 12372 170503 .27 Woodlake Yacht Club NC OS-03-17 0950 K.Keenan Fed -Ex 0.3 12373 170503 .28 TVA* Allen Fossil Plant -001 T N 05*03-17 0950 K.Keenan Fed* Ex 0.2 12373 170503 .29 TVA* Allen Fossil Plant -I ntake TN 05-03-17 0950 K.Keenan Fed* Ex 0.1 12373 170503 .30 TVA -Allen Foss i l Plant -Upstream TN 05-03-17 0950 K.Keenan Fed -Ex 1.2 12374 170503 .31 Carolina Beach WWTP NC 05-03-17 1152 J. Sumner Dash Courier 0.3 12375 170503 .32 OWASA* Mason Farms WWT-:> NC 05-03-17 1215 K. Keenan L. Keenan 4.0 12330 170503 .33 Jefferson WWTP NC 05-03-17 1215 K.Kee nan L. Keenan 4.0 12331 170503 .34 West Jefferson WWTP NC 05-03-17 1235 I. Sumner TVA Courier 2.1/1.7 12332 170503 .35 TVA* Sequoyah Nuclear P l ant-101 TN 05-03-17 1235 J. Sumner TVA Courier 2.2 12332 170503 .36 TVA-Sequoyah Nuclear Plant* Intake TN SOP G4
• Exh i bit G4.2 , revi si on 0 1-03-12 I . ' Ctf:ont: 1'V Ai. * ' ilntiilr

.. " Comp BlOMQ'NJTQRINGCHAlN QF. :CU$'.rt>l>Y k£CORD Bniir.omnen .tal Testin&-SQtut.km t 1nc .. 3 Dep'1t* SMet.. bett:v ered tl f VPS.* : etient Plir.ne:* .\ . -:F.ax f s-s---'i1 t3t< J.. <-/-.OJher 5aote .. rt-.i * * * * . . .

• w; . .. .. *

... '. *.' '.** *<ilt/if#A"" Btln. -. 111(1* i'llP.W: '

• 1.oti ' 'j " i fttf'. XI hlf*
• 1* ... *1* " *r 4 \' : : .* ... ;

ft" :lnstmorkrilii i nu iii all ArCd eJSt:ept m the*'41Abl>Ta(Qry santplctt.ate by Sltldnl them at b'C D?<l , tbem '" kc. '.f'h1t hbkl time for ttcb . 6:om dtc time Of TI{eteRln. plea. and khip In Wf/i <<mt t114 will receive tllC with amplt rime to1TI1hiµc whhln that. time fra111e *. sb1ppe,d . ovt;trughl Qn .Ftlday 'i'ia FlldBx.'Qr UMJ must be RU!tkad fbr 68turdll.Y .dettvtry Wlf I not 1ltl'fVC unall the following Mond'ay. '; Whole Effluent Sample Receipt log Page Sta 'Sample 1emperature performed uslne Samp l e Rece iv ing T he r mome1er: SN Date Time Received Received *Sample Project Sample Sample name and description State Comments Received Received by from Temp. (°C) number number 05-05-17 0943 K.Keenan Fed -Ex 0.9 12347 170505 .01 Apex WRF NC 05-05-17 0943 K.Keenan fed* Ex 0.1 12348 170505 .02 Belews Creek SS NC 05-05-17 0943 K.Keenan Fed -Ex 3.3 12349 170505 .03 Daikin Appl i ed Americas NC 05-05-17 0943 K.Keenan Fed* Ex 0.8 12350 170505 .04 Dan River CC NC 05-05*17 0943 K. Keenan Fed -Ex 2.1 12351 170505 .05 Davidson Water WTP NC 05-05-17 0943 K.Keenan Fed -Ex 0.3 12352 170505 .06 DobsonWTP NC 05-05-17 0943 K. Keenan Fed -Ex 0.9 12353 170505 .07 James Loughlin WWTP NC 05-05-17 0943 K.Keenan Fed -Ex 0.3 12354 170505 .08 Performance Fibers NC 05-05*17 0943 K. Keenan Fed -Ex 0.4 12355 170505 .09 Roseboro WWTP NC 05*05-17 09 4 3 K. Keenan Fed -Ex 0.2 12356 170505 .10 Scarlett Acres MHP NC 05-05-17 0943 K.Keenan Fed -Ex 0.3 12357 170505 .11 Spa(ks Road WTP NC 05-05-17 0943 K.Keenan F ed -Ex 0.7 12358 170505 .12 Elementis NC 05-05-1 7 1220 K.Keenan Dash Courier 0.4 12375 170505 .13 OWASA-Mason Farms WWTP NC 0 5-0!>-17 1220 K. Keenan Dash Cour i er 0.7 12346 1 70505 .14 Rockingham WWTP NC 05-05-17 1240 K. Keenan J. Brewer 1.4 12330 1 70505 . 15 Jefferson WWTP NC 05-05-17 1240 K. Ke e nan J, Brewer 2.4 12331 17050S .16 West Jeffe r son WWTP NC 05-05-17 1230 J. Sumner TVA Courier 1.1/1.3 12332 17050S .17 TVA-Sequoyah Nuclear Plant -101 TN 05-05-17 1230 J. Sumner TVA Cour i e r 0.9 12332 170505 .18 TVA -Sequoyah Nuclear Plant -Inta k e TN SOP G4 -E x h i bit G4.2 , rev i sion 01-03-1 2 -, I l Page I of 7 Chronic Whol e E ffluent Toxicity Test (EPA-821-R-02-013 M e thod 1000.0) S pecies: Pimeplzales prom e las Cli e nt: Tennesse e Vallev A utboritv Facility: Seguovah Nuclear Plant NPDES #: TN0026450 Proj e ct#: Di/rdio11 prepnratio11 illformntio11

D il ut io n p r ep (%} 1 0.7 2 1.4 4 2.8 Effiu c nt vo l u m e (m L) 267.5 :i3 5 1070 D il ue r , 1 volume (mL) 22 3 2.5 1 96; 1 4 30 To1al v olume (mL) 2 500 2 50 0 2 50 0 T est organi s m illformatioll:

Org an i sm so ur ce: In-ho us e c ul t u r e Age: < 24-ho u rs old Spaw n da t e: o"\* 1.."\* \""\ Hatch d ate s and t i mes: \\. \\00 Transfe r ve s sel i n fom 1a tion: pH-85.6 1 00 2 1 40 2 50 0 3 6 0 0 250 0 2500 'lJ s.u. Tempe r at ur e == c.'.\..'\ " C Ave r age tra n sfer vo lu me: < 0.25 mL C ounty: Hamilton Outfall: 101 C omm e llt s: Each co n centratio n was UV-trea t e d for 2 minu tes to r e mov e pathoge ni c In t erfere n ces. T es t ill{ormation: R a ndomi zi ng t empl a t e: PM.e. '\eu..D\a) I n cu ba tor n u m b er a n d \(.. sh e lf l ocation: Ar t e 111i a CHM nu mb er: CHM9 14 Dr y ing information f o r weight determillntiou: Da t e I Time in ove n: l'l!t*CA"l-, I n i ti al oven t e mpera ture: l.i'\. (. Da t e I Time o u t of o v en: !f\C::. ... D*l'"l nA-..n F ina l oven tem p e ratur e: L..Q -c: Tota l dry i ng t i m e: Dail y fe edin g mu/ renewal i uformntiou

Dny Da t e M ornin g fee din g Afte rnoon feeding Test i ni t i a ti on , Sa mpl e n um bers used MHSW renewa l , o r bat c h term in at ion u s e d T im e A nal ys t T ime A n a l y st T im e An a l yst Ou tfall 1 0 1 In t ake 0 0 5-02-17 01..10 11 \"1.. :io w l\ ri<.t\ \ * (C)

\. o-\ o"\< 'llo* n" . -I 05-03-1 7 0 "-l:>O I \ 1.0C w V\ \\O$()l. \\llS0\-0'\ 2 ".r .. .. 05-04-1 7 n .. o&SO h o"-*i.s.*,,6 3 05-05-17 O\o\'I;) 1t{ \'1.lt:) l.I oSS"L ({' rt o.s o :a.i.. 4 05-06-1 7 \-1 \,{ I"'\ OS.OS. \\ QS.*01.*\1 5 05-0 7-1 7 <JI'-\() }{ \) M OC\S"L. \"'\ l"IS0 S'

• r\ ,,osa;.

6 05-08-17 .. l \ OS'?)?) w \1 nc;.*(ft.*1 , 7 u , V\ >.\ 05-09-1 7 04S-'\ Co11trol i 11formatio11: Vl* Ac c eptance crite r ia Summm y of test e ndpoint s: % Mort a li ty: 01. S2 0% 7-da y LCso > 1001. Ave r age weig h t pe r initia l larvae: NOEC 1007. Average we i gh t pe r s u rviving l arvae: o.t..'\e 0.25mg/l arvac LOE C lO() 7. C hV "'> lN\1. I C15 > 1001. Page 26of94 SOP A T 20 -E xhibi t A T20.3 , revis i on 11-01-1 4 .: ,..--........_..._ Page 2 of 7 Species: Pimepltales promelas Client: TVA I Sequoyah N uclear Plant. Outfall 101, UV-treated Date: 05-02-17 Survival and Growth Data Day CONTROL 10.7% 21.4% A B c D E F G H I J K L 0 10 10 IO 10 10 10 JO 1 0 1 0 JO 10 IO ] IO IC> IO J {) I() IC l t> to IQ co tO 2 /\) to IO 10 10 lD IQ lO lD I CJ 10 f() 3 10 10 io 'D 10 lQ l () IQ '0 I() lO t() 4 tD ID 'D 'D ID IQ ft) ( (J I t) '0 l () 5 llJ 10 ID !() (() I() I\:) ID ID 11) l(J co 6 ID ID ID It) 11;:} It> 'a l(J IQ I C 10 10 7 It> I l) l () I 0 I() I 0 /\) ID IO /0 t lJ IQ A -Pnn \S.o3 \s.ec (5.ot l5.'4S-Troy co l o r co de:: >> L 0!0.XJrC.. llP. 03 l "1.1 ... Annlyst: iJ..., Dnl c: O"'*cii* 1:r ll =Pan+ Lar vae '[>'.;ht (m g) Analyst: J.1.00 a.135 Date: 01<5.) I .1):

o.q l

1,l.4$

l3, I(,. C = Lar vae weig ht (m g)= B -A (.. 11. t. "'\. 1.<f

!> ,.o'-l "\.O\ '-*,., "'\.oc ,.o\ lo. ,,,\ Hnnd cnlcul nlcd. A r rn ly st: Wei g ht per initial number ofl arvnc {m g) = C /I nitial numbe r of lar-vn e ,.../ .).. t:-,& O" 'V' """ ,...<>' # "() Hand c nlculated.

,;> ""' " " " "' "' "° C>' A n a l ys t: Q. o* o* o* o* o* <:) o* er Average wei g ht per Per cen t reduction 0-"'°" &-o.\o'\lo O.""\"l."b -1. initiul numbcr of rrom co ntrol (%) " lar vae (mg) -Comment codes: c =cl ea r , d =dea d , fg =fu ngu s , k =k ill ed , m =m i ssing, sk =si ck, s m =unusually small , lg= unusually l arge , d&r = decanted a nd re turn ed. w = wou nded_ Comments: Page 27 of94 SOP AT20-Exhibit AT20.:;, revis i on 11-01-14 l -, l J J : r;::::t c:-;::l i "--(' ) .... Species: Pimeplwles promelas P age 3 of7 Client: TVA I S e quoyah Nuclear Plant. Outfall 101. UV-treated Date: 05-02-17 Survival and Growt!t Data Da y 42.8% 85.6% 100% M N 0 p Q R s T u v w x 0 10 10 1 0 1 0 10 10 10 10 1 0 10 JO 10 1 /0 10 IC 10 ID co lQ l {) tO 10 10 IQ 2 fO IQ to t \) L {) l \) ta 10 { {) I\) '0 IC) 3 I 0 ID 10 lO lO 10 I() lb lb ID 10 co 4 I 0 l () ID /"{) [ 1) lD 10 LO 10 tO lb ro 5 '{) I (J 10 l \} I iJ ID 10 ID 10 10 IQ 10 6 I 0 10 IQ I () /Q 10 ID l'\j ID 10 ID 7 10 ID It:> 10 /{) lb CD 10 I() /0 tO *D A ,. P a n w ei g ht (m£t Trny c o l or cod e:: yciqoo*.s..e-r '5-3'-t llP.18 llP.Z"' 14.01 ""l-l'i.61P 16.l"l 1 IS.lo\ An n l ys t: D a te: 13 =P a n + L n r v n c weig ht (m g) ).ol.1o3 :i1. a,._qo c:it0.11 A n a l y s t: a.\.'-'V> D ate: 06. \\. f.}--C =Larvae w e ig h t 13-A ... t,,,\o1. ,, \t:\ 1..s1 c.., lolo 1oA'-\ "'.'-\'\ H n n tl c n lcnl n tetl. ti Anal ys t: W e i gh t per initi a l number o f l a rl'ae (m g) = C / l n i t ia l numb er o f la n*ae "' .)-. '-:1"' 'I> :t' "' tf, tV q. °' '° IO H an t! c n lculat c tl. ..... -.g -.&) "° 'I> \0 <). " ""11 "\O Cf r;;;;,' c' A n n l yst: o* Q' 0 o* '\). o* i:5 Avera ge wei g ht pe r P e rcent retlu c l i o11 0. IJ/'t '-\. \ 1. O* 1..\.\1. s.. '-\ 7. i n i t ia l n umb er o f from contr ol (%) l a r v nc Com m en t co d e s: c -clear , d -dead, f g =fungus , k = k i l 1cd , m -missi n g , sk -sick, sm =u n usuall y s ma ll , J g= unu sua ll y la r ge, d&r = d eca nt e d an d r et urned. w = wo un ded. Co111me11t s: Page 28of94 SO P A T20 -Exhi b i t A T20.3. r evision 11-01-14 J Page 4 of7 S p ec i es: P ime plr a l es p r o m e/a s C li e n t: TVA I Segu ova h N u c l ear P l ant , Outfall 101. UV-t r eated Dat e: 05-02-17 S ur v ival mu/ Grow th Da t a Day 1 00% In take C ontrol -No n-t rea t ed y z AA BB cc DD EE FF CG HH 0 IO JO 10 IO 10 10 10 1 0 I (0 10 I 0 10 IQ to 1 0 ( () 2 t o I \:J to co t o to ID 10 3 ID f O I\) 10 1 0 10 I CJ IQ 4 lD ID I D ID l b /(j IQ I Q 5 l'O I {) ID 1 0 I Q I Q I (J t o 6 IQ I D I I) 10 f(J J () tO IQ 7 <1'-t 1$Jo\ 10 "'.,c. I t:> 1 0 J O {() f\) A= J>nn weight (mg) l lti.16 Tray color code:: lSA 4i l'-4. 143 }5, Lt4 l S.e>o \5.82. \S.0'.P 15:-:k) Ana l yst: ,.!{., Date: 3 =Pan + Larvnc weight (mg) /H>.IP 8 a1.43 '"3 .lo5 Analyst: Date: C = Larvnc weight (mg)= B-A (,.So t..os (o, i& c,. "l. t.1J .... Ir;.'\ Hnnd calculated. Ana l yst: Weight per initia l number of lan*ac (mg) = C /Initial number of larv:u: '11,.,,'V' a."'IJ tt-. c,,O 6-' ....,o v Hn n d calculntcd. £.; " ""° .._, "° '° "" o* Ana l ys t: '\:)' o* o* o* o* \:) o* Av e rage weight per Percent reduction 0. (., 1t:\ initial number of from control (%) &\. C)1. lnrvne (mg) 0* Comment codes: c = clear, d

• dead , fg = fungus , k =killed , m -missing, sk = sick, sm = unu s ually sma ll, lg= unusually large, d&r =dec anted and returned, w =wounded.

Co111111e 11 ts: a JJ Page 29 of94 SOP A T20 -Exhibit A T20.3 , revision 11-01-14 Envlronmllntal Testing Solutions , Inc. cV.1 C unlrul. No1Mf't'tUt'tl C oru1ol, UV*lf" U IC'd IU.7% Zl..-1% 4?.S% 35.6'Y. toO"/* lntuk.C' ill!!!il!!.l!ll 'ISO' l11r-1 ,.,1 0: l 11t?kr: l>unn£ll 1!\ M S U \"i!IUC': 1*1\l U: cc 1111 ££ ff " u c I) f c II I J K I. M N 0 r 0 II s ,. u \I w )( " z Ail uu 1nhbt 11*"*bt.t*r t1n1I IUtmhrr t1 f bn llC lann 10 IQ 10 IU 10 v 10 10 10 10 10 1 0 10 1 0 10 1 0 IU 1 0 10 1 0 10 1 0 1 0 1 0 10 1 0 I Q IQ 10 10 IQ 10 1 0 10 10 10 IQ 10 10 10 10 10 IQ 10 10 10 10 10 IO IQ 10 1 0 10 10 IQ 10 10 10 IQ " 10 IO 10 10 0.0511 7.J 0.0605 8.7 ". 1'1ri .. irt;::llt (n11.t1 ISSO 1116 1 570 I S l6 1 6 56 I SOJ I SIO 11.so 1 60J ISQI 14 14 IS 06 , .. JJ IJ6S IS4S ll 76 IS J4 l b 2 1:i 1603 1 6 ?b IS 74 l.t.K'J 16 21 IH6 IS 17 14 ** 1'61 IS 11'1 14 6J IH7 1605 MD PMSD TVA I equo ya h Nuclear Plant , Outfall 101 Ma 02-09 2 01 7 Pi m ep hfll es pro111e/11s h ro ni c Wh ole Ef'nuc111 Tox i city Tc *1 El' -82 1-R-02-0 I J. i\lclhotl IUOU.O Qn:ililv *0 111rol Veri ficati o n of Data E ntr y, Calc ul u ti o n , 1111<1 '1:1tislicnl A nnl yscs N0t for Comph;inci: l.abo nnory QC 8*1";,1 11* l...lfl M' L3 n*"cl;:J\*4f'll lt l \\'(1t.k 1 i S.n h titt M o.n1*riltltll Cr<lllc'"'l *I W flj:'h l l l11l1l:lln1Jttlw r "* ll*A fu 11nl K't t{rbn:w,n 1i: 1 !Min n u*n htt u r "' ...... , ....... ,-...... ..tl.1."'lM" l t11 1ll l.1nw(*tr:l _,., , ... r.'..) 2l I? 6 J:? 061? 0"32 2l 6S HJ 0 7$} Oo*6 qi IJ7U 22 1 6 6 3Q 0700 0 0)0 ??J9 669 0669 0*69 21 SS 6l2 0 6)2 0 6)! 2 l79 7 23 072J 0 q *' 0 721 22 JS 732 0 7J? 0 712 22 8l 7 Q) u 70J 0 70) 23SQ 700 0700 0 700 2] 07 70.1 07().l 06% IK 0 71).l 22 O? 701 0 701 0701 20.91 6 77 0 677 0677 22 JC: 7 JO 0 7JO 0 llO 21 45 7 0 1 u 701 t} 7?J () 0 701 20.S 4 6 &q 0630 068'1 2.l lt 1 77 1 0 771 0 771 22 6) 6S1 tl 687 061 7 l l 76 0.6.1? U6 6* l 1 06 H 22 9(; tH 1.2 0 66! 01)6? 111; 6 g; 06$4 6 JJ OulJ 06H 22 9] 7 l'J 0 71q 0 (){>') '*I 0 719 21 .1 e 6H Q 6S7 0657 12 IJ J 664 Q666 0&66 21 JC 6 44 Q(>t 4 06-H 21 6E 6 49 0649 06J U ll OllJ O W7 1 6?7 0 627 u &21 6 )7 0637 06)7 l2 4 S 6So 0650 0650 20 61 6.0l 0672 OM! 62 ll 4) S% OS96 OS% 22 9] 6 8 1 u 681 0 683 M 1n11nu111

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,..,_ .. _, .. ....._, ,., 0.679 10.6 No t "r1, tic:t bl c 100.U Q.69S 6S N'o 1 u p1*l itu blt> 100.0 u.696 OJ 100.0 U.7 1J 5.11 .J.o 100 , 0 0.669 .l.I 4.1 1 0 11.0 0.660 * .a 4.1 1 00.11 0.6J? SA 97.S o , u s 6.7 9.0 PMSO 11 .i p111.--cmo n PMSO is the r11101111u11 11 ,..,'fccn 1 dtlf.:u."tK.: .. '1 1 t he c onuol ru ld d1J1 <k-cla1\.*d s:ti;tu f rc,ll\1 m11 \"-hok d Ulh!l'l1 I Olo C1t y resc l.o\ver l>MSD bolWd dcli * .,mmcd b) USEPA (10th t>cre .. t 211.. llpp..'f rMSO bound dc 1 crn11ncd b

• U SEl'A c 90t h pc r c.11 11 lc l Jm. l.oWl!r 3.0d *11p.:r D bollnds ,\'\..h! dl.'.1.:11ntocJ from th.: 1 0ih .l 1d U<k h Ptr.:.:l\f\k-.

MtlCC l l\"clv. d;ma f r tJm El'1\°> \YET lttt£1l:1hor:iaory V:vt.ihl ln\* S111dy (t J SPl 1 A 100 1:\ t 1 1\f: p , >OO lt.) TVA/ equoyah Nuclear Plant , Outfall 101 Ma 02-09, 2017 Start Dat e: 51212017 End Dale: 5/9/2017 Sample Date: May 2017 Comment.s: UV-T reated Conc .. 0/o 1 2 Non-Conlrol 0.6320 0.7830 UV-Control 0.6320 0.7230 10 7 0.7000 0 7040 21.4 0.7300 0 7010 42.8 0 6870 0.6420 85.6 0.6330 0.7193 100 0 6 44 0 0.6490 lnlal<e 06500 0.6050 Conc 6*/o Moan N*Moan Non-Control 0.6785 0.0726 UV-Control 0.6975 1.0000 10.7 0.6955 0 9971 21.4 0 7228 1.0362 42.8 0.6688 o 9588 85.6 0.6688 o 9589 -100 0.6393 0.9165 Intake 0.8348 o 9 oo Te s ts Stat i stical Ana l y e Larval Fish Growth and Survival Tost-7 Day Growth Test ID: PpFRCR Samole ID: TVA I SO N Outfall 101 Lab I D: ETS-Envi r. Tes l ing Sol. Sample Type OMR-Discharge Monitonng Report Protocol: FWCHR*EPA-821-R 013 Tes t Species: PP-Pimepha l es promelas 3 0.6300 0.7320 0.7010 0.6890 0.6620 0.6570 0.6270 0.5960 Mo.an 0.6785 0.6975 0.6955 o 7228 0.6688 0.6668 0.6393 0.6348 4 0.6690 0.7030 0.6770 0.7710 0.6840 0.6660 0.6370 0.6880 Willie hypotn esfs test results indicate a s l gnlflcant dlll e rence n growth for the 100% OuUall 101 samp le, tho PMSO w as below the lowe r bound (12%) os1ablishod by EPA. Guidance In EPA 833-R-00.003 (June 2000) S oclfo n 6.4.2 for determ i n ing lh e NOEC was followed, and ii w as concluded that lh e hypothes is lest yielded a Type I Error. T ransform: Untr;af1Sformed 1-Tallo d I s otonic Min Max CV°!. N I.St.at Cr l tlcal MSC M e an N*Moan 0.6300 0.7830 10.602 4 0.6320 0.7320 6.497 4 0.7053 1.0000 0.6770 0.7040 1 790 4 0.094 2.410 0.0511 0.7053 1.0000 o 6690 0.7710 5 046 4 -1 190 2.410 0.0511 0.7053 1.0000 0.6420 0.6870 3 145 4 1 355 2.410 00511 0.6666 0 9483 0.6330 0.7193 5 445 4 1 352 2.410 0.0511 0.6688 0 9483 0.6270 0.6490 1.492 4 2.746 2.410 00511 0.6393 0 906* 05960 0.6880 6.718 4 St:>tlst i c Skow Kurt Shapiro-Wolk " s Test ind i cates normal d i slritlulion (p > 0.01) 0.97487 0.884 -0.2395 0.86461 Bartlett's Test ondocates equal v riances (p z 0 14) 8.38396 5.0863 The control means are not s19n 10car'\UV ditleren1 <!! = 0.67) 0.<:<:695 2.44691 Test !1*1.all, 0.05! NOEC LOEC ChV TU MS Du MS Op MSB MSE F-Prob df D unneU's Tes t 85.6 *100 92.5203 1 16822 0.05112 O.C*7329 0.00341 0.0009 o 01612 5. 18 T reatm ents vs UV*Con l rot 1n1orpo1 auo 111 2 00 Po i nt % so S6% CL(E.>: E) Skew IC05 42 096 24.500 17.808 122-928 0.4432 IC10 >100 IC15 >1 00 1.0 IC20 >100 0.9 IC25 >100 IC40 >100 0.8 tCSO >1 00 07 .. 06 "' c: 0..5 0 Q, 04 .. " 0: 03 0'2 0.1 0 0 -0 1 0 50 1 00 1 50 Ooso-Res pon so Plot 09 o.e 0.7 ... 06 'i * * * * *

• __ . __ . _ . _. _____ -_:-.§::-_ ::-. ::-. 7.

Ha ll. 0.05 level of significance e o.s " :-0 .. 0 ,_ 0.3 0.2 0.1 0 0 c 0 u .: 0 z g co "' 0 "' 0 c ;;; "' .!! 0 s 0 > :> Page 31of94 TVA I equo ya h Nuclear Plant Outfall 101 -Intake M a y 02-09 , 20 17 Tcs 11n9 SoluUon1. Inc. Start Date: 5/2/2017 End Date: 5/9/2017 Sample Date: May 2017 Comments: U V-T reated C on e-% 1 2 Non-Control 0.5320 0. 7830 UV-Control 0.5320 0 7230 10.7 0.7000 0.7040 21.4 0.7300 0.7010 42.8 0.6870 0.6420 85.6 0.6330 0.7190 1 00 0.6440 0.6490 Intake 0.6500 0.6 0 50 Co n e-% Mean N-Me an Non-Contro l 0.6785 0.9728 UV-Control 0.6975 1.0000 1 0.7 0.6955 0.997 1 21.4 0.7228 1.0362 42.8 0.6688 0.9588 85.6 0.6668 0.9588 100 0.6393 0.9165 *intake 0.6348 0.9100 A uxilia ry Tes t s Statistic:il Ana l yses L a rv a l Fi s h Growth a nd Surv i v a l T e st-7 D a y Grow t h Test ID: PpFRCR Sample ID: TVA I SQN O utfall 101 -Int a k e Lab ID: ETS-E,vir. Test in g Sol. Sample Type: DMR-Discharge Mon i tor in g Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales p rome las 3 0.6300 0.7320 0.7010 0.6890 0.6620 0.6570 0.6270 0.5960 M ea n 0.6785 0.6975 0.6955 0.7228 0.6688 0.6688 0.6393 0.6348 4 0.6690 0.7030 0.6770 0.77 1 0 0_6840 0.6660 0.6370 0.6880 Whil e hypoth e s is t es t results indi cat e a si g nifi c a n t diffe re nc e in grow t h fo r t he i ntake sampl e, t h e PM S O wa s b e low t he lower bound (12%} es tab li s he d b y EPA. Guid a nc e in EPA 83 3-R-00-003 (Ju n e 2 0 00) Se ct i o n 6.4.2 fo r d eter m i n i ng the NO E C was fo ll ow e d , a nd i t w as conclu de d that t h e h yp o th e s i s te s t yie ld ed a T y p e I E rr o r. T ra n s fo rm: Un tr ansformed 1-Tailed M in M ax C V% N t-St at Cr i t ic al MSD 0.6300 0.7830 10.602 4 0.6320 0.7320 6.497 4 0.6770 0.7040 1.790 4 0.6890 0.7710 5 048 4 0.6420 0.6870 3.145 4 0.6330 0.7 190 5.425 4 0.6270 0.6490 1.492 4 0.5960 0.6880 6.718 4 2.017 1.943 0.0605 S ta ti s ti c Cri ti c al Skew Kurt Shapiro-Wilk's Tes: i ndicates normal distribution (P > 0.01) F-Test indicates equa l vanances (p = 0.92) 0.95314 0.749 -0.4316 -0.9929 1.1 2 947 47.4683 The control means are not s i gnificantly different (p = 0.67) 0.44695 2.4469 1 Hypo t h es is Test (1-ta il , 0.05) Homoscedastic t Test indicates significant differences Treatments vs UV-Control 0.2 0.1 0 .l: c: 0 <.:; c: 0 z Page 32of94 g c: 0 u > ::> ": 0 MSDu MSDp MSB MSE F-Prob d f 0.06046 0.08668 0.00788 0.00194 0.09029 1 , 6 Do se-R es pon se Pl ot "" "' ... 0 0 ), __ Page 5 of7 Specie : Pimepftnles promelas Date: 05-02-17 Clien t: TVA I Scguovah Nuclear Plant, Outfall 101. UV-treated D *1 Ch . at JI emts t ry: Day (Analyst identified for each day, pcrfom1 cd pH , D.O. and conductivi ty measurement s only.) 0 I 2 A nalyst y.-1.J.-I>--, ........ "'v Jv Co n centration Parameter ----. *' .: ,. -.. . . " ..... .......... ---. -f * . -, -::: ... --* .....c..o.: .. . . -oH (S.U.) a. 1'\ (b{}l.a ':\..u" e.ov "".},"""' DO (m!!.11.) Ila t;\-.f.O '+A Conductivity -322-. .3Z6 ' 01C\ 1 ........ __ * *...-. ** t '1 CONTROL (µmhos/cm) I , .... . .... *Alkalinit y i':' *;\'" \p...\ .. *. '"'-...-N'"' ..... *;*..-*** UV-tre ated (mg CaCO;/L) *:*'*'**-**-**:. . :*% . :.**** .. . 'r.:. . *Hardne ss . ..

• --. . ---.. , . &l.o ' . (me C aC 03'L) -=-* " *Temperature

("CJ \.'-l * \o \..'\*lo "1:-\..J pH(S.U.) c&.\6 ll.f.>'t e.4"\ ')._\JI\ DO (mwL) 1\-.C\ '+.l 10.7% Con du ctivity . ' .:* 3l0 '* . 3oto fomhos/cm) ' .. " ' > -. . ., " Tempera t ure ("CJ '1.'\.& '"\.'-\*l '"\..'-l-1 "\.'-\. '\ "'\A.A pH (S.U.) e.1v ra-' o '.\.\tl DO (mwL} 'l.q \O.'l {J).v 21.4% Co ndu ctivity --. 2>12. I> (J.lmhos/cm} ut\ ' . "'Temperature (°C} "\.'\. << \."\* 1 \1-\.\ LS:o '"'-"'* '{ nH(S.U.J S.14 '+.\O\ ece ..... DO (m!?/Ll I}.:::; '+q (/1 \ 0.0 in< 42.8% Conduclivity --"" 1-2e:::J.o I UPO ... --"'Temperature C°C) i.'\ . .g \..'{.'\ .,_ .. t. ,.i{.I.. pH (S.U.) '\..t>S e>.\ \ ao::\ DO(m!?/Ll 'l-.5 'tY lll'Z. 0.0 l4."1-85.6 Vo Conductiviry ,q' 16<.o --. -*Temperature (°C) t.*V1 "'\!-\. '\ 1.."\-1 '1.'S. 0 pH (S.U.} 0.\$" /}.PM "+ *,;f 0.0'-l "\..5tP DO (mg/L) {Jj.J) t:\.&I u,?_ 6.o <J1.eJ Conducti\'ily -.;;. ... I l,/J::) .. (u mho s/cm) .c -.. -. *A l kalinity . . -. j ---...-...... - 100% S(\ .. toS (mg CaC03'L) . ! -. . .. *H:t rdncs s t,...\ --:.-.," .-.. . ]ij -. -vl.o (mg CaC03'L) .. **: i i.* . -*' . ::.*: . .. *TR ch l orine (mg/L) < 0.10 .. " .-..... .... --r..-:-* . -;-*:--<o .10 ,, ., .. '"' . . . ' *Temperature (°C) 0 l."\* 1.'1. '\ "L"\. cf "\."i. \ '"l.'{. '° pH (S.U.) 6.11-' r1dl'< ll1Al A03 'l."53 DO (mg/L) , .J..l.P l.l)."'\ A.O Conductivity .. tll3 . .. . t 146"' . ., 168 -,'fl; (umhos/cm) . r . .\, . -.i...:;. * 100% *A lkalinit y -. . . .-:-* . . . (9\ .. (m2 CaCO;/L) . . lntal<c *; . .... -* *Hardne ss

• I I ... . .. ,, (ma CaCO;/LJ . lab . -.,_,_ . ------'* 1&.1' *TR chlorine (mg/L) <o.tO ; <0.10 -*Tempcrnturc

(°C) L.,"(.'i '\.-( .c\ '1."\. s n. \ 1,\.(, 'i Initial Finn i Initial Final Lniti:t l Final ,......... mpcraturcs pcrforlllcd at the lime oftest in it i atio n , r enc' al or termination by th e analysl ide nti fied in the Da ily Renewal Information table located on Pa g e I. ...... w iR,lk inil y, h dness and tot;i l residual chlorin e perfonned by t h an a lyst on rhc b e nch for each and In h c n c h Total residual c hlori n e was performed on n on-treated Outfall IOI and l nrakc sam pl es. Page 33 of94 SOP AT20-Exhibit AT20.3, revision 1 1-01-14

• -c--:::J lf=l -I . Spec i es: Pimeplwl e s promela s 1en : eauova uc ear an . u a . Cl' t TVA I S h N I Pl t 0 t[ JI IOI UV Page 6 of 7 -trcate d D ate: --05 0 2 17 D ay (An a l ys t i d entified for e a ch day , p e rforme d p H , D.0. a nd co n d u cti v i t y meas ur emen t s on l y.) 3 4 5 6 Analyst v---.,_ "'--IA-.Iv J.f-..1 C oncen-P a ramete1* -. -. -, , . :. -. -. trntion ' .. [[ -i -, _. -*-nH (S.U.) f?>.oS

'.}.§ B f'l,.. 0.\"' rJ.Sl-DO (m l!/Ll g.o c;c.. 2..-"'* -s 'l.< !'.}Pf (jf,z... Cond u ctivit y 22.LP i r::t -; 3\2-. 32\ C 111nh o s/c m) . CONTROL --.r --' -*---*Al ka linit y .. ---*---..... U Y-t reated \cl1--(mg CaC O JILl .. ... "!_ . . "' --_.!,._ *llardncss & '4 ..----, "' . I (m a C a CO;/L) -. -*Temperature (°C) ""\.. ...... \..-t*"-"'U\* '"\.. .. \. '\ \..--\* \ \.."'\. "'\ "\..4,.1-- riH (S.U.) g' *\ 2.-'l,,o..\ t'\ 'l.e'-t 0.\1.p DO(m!!IL) (JL() 1..1-<l. \ lnVl e.o ((), '2... 1 0.7% C o ndu c ti v it y .. -210 3"1 3oS I (j am h os/c m) *Temoe*rnture (°C) b-\.\ '-'-t*S ""\.'\.<\ .......... \o \.."'-*" pH (S.U.) e.o4 -J.-:n-.\\ ".t*-5\P

• • • 1.JA DO (m lifl.) -:J.r-+-

to.+ 6.J.J "".},"' Jl..tJ <.P \ 2 1.4% Conductivit y . ,'.' *' .. 2°t?, (u m h o s/c m) -I -W' *Temperature (°C) -,s...Q "'\.'l

• c; '"\..'-\*

\.:. '"\.-l*'-' riH (S.U.) ';}.°'S' ".1:*:n- 'l.o..l e.O'\ e.\e, DO (m2'L l 6..0 J..1-I0.6 t!>.O "),,q 14.0 42.8% C o nduc t iv i ty a1..1l . ,,. .... -I* ..... * ... ' -* '* ' {11111h o s/cm) -*. *Temperature (°C) "'\S. 0 "'I .... la \S.o -..,'-\. b -U-\. "'\A*S '"\.Ci. .o '-"\* ,_ 1>H (S.U.) '-l-A'l ""J..'1-r OIJ"'IL.4 ".\-.0(0 0,\G:\ DO (m g/L) 0.0 'J.'l-({ . ' I0,1..D es.1 ':).ta /.}.q <P.o 8 5.6% Corul u cli v ity . .* .., --ll\) \Kl l'13 l q l ., (µmh o s/c m) --- C°C) l.S.. \ "\_ '-t

• le, u.a L."\.1 l.'\.'\ L.!.\*l-. ""\..'-\* "L pH (S.U.) f:')O?i. ?."'=Iv "iLD 't 01'11.11 '+.A".} e.\a DO (mlifl.) e.o l.t-l e.o 9-0 Cond ll ctivit y . . \ lt>'":r 11.tS-. (µmho s/c rn) --* .-i..;. *AJknlin i t y .. -;; -' r 100% -. ""°' ' (m g C aC O;/L l --. . ---*ll:irdnc ss -! -,* . -(m g C aCO;/L) .' --,_ ---*T R chlorin r (mg/L) -. -I f"l.I(') --'. ' -. .4}-... --' '* *Tempera t ure (°C) '"\S. \ "\!-\. s -u;. \ -r-\*.S L.S.O "'t.L\*1-

\ DH (S.U.) -'J, , f'C( 0.0""} .tlC\\ {:).la DO (m l!/L l so -i...v 'J..CJ '6.o "l'l--:JA ':).Cf Condu c t i v ity 1 .. . ; -(1m1h cs/cm) iuie -. : l '4 \ 11v -' ---*-*-100% " Alk:ilinity -. ' . '; ---.. -,.-. -<o\ . . Intake (m e CnCO;/L) : J :-;i , ' _li . .: 'Ji. -. -. ., . . -*Hardn es s f' -. -' .. " -, , -"' 10 I* .. (m g C a C O ,;/Ll .,..-:1 -i' . '31 I* *-. . -* *TR clllor i nc (m g/L) LD*\0 . _-----: -,. ., . -./ . ,, *-' -*Tempera t ur e (°C) "l$..O l..J.-lo 1.S* \ 1,.'{., 1..'-l* 1$. \ 1..'"\.S Initial Final lnitial Final Initi a l Final Initi al F in a l llUf t n '** : w i't c 1 p eratt1 r es p c r fonn ed at t he t i m e o f t es t i nit iatio n , r en ewal o r tcm 1in a 11 o n b y th e an a l ysl i dcnt ilie d m th e Daily Renewa l ln fo nnation t a bl e l ocated on Page I. "-"*v* .. A l k a 'ni t y , h ar dn ess a n d t o t a l r e s i du a l c h l o r i n e perfor med b y t h e ana l ys t i d e nt i fied on lh e benc h sh e e t s p e c i fic for each a nal y s i s a n d to lhis bench shee t tf.J>y: . T o t a l resi d ual c h lor i ne was pe r fo rm ed o n 110 11-tr c a t e d O u t fa ll IO I a n d lnlake sa m pl es. P e 34 of 94 SOP A T2 0 -Exh ibit A T20.3 , rev i sion 11-0 1-14 : ,_ .. -' .. Page 7 of7 Species: Pimep h a l es promcla s Client: TVA I Sequoyah N ucle ar Plant, O utfall 101, UV-treated Date: 05-02-1 7 D *1 Cl a u y 1 e1111sfrJ1: Day (A na l yst identified ror each day, nerfonn cd pH , D.O. and conduc t ivity meas\lrcmem s onlv.) 0 l 2 Analyst if..; ..}---iJ...,; IA 1--,..)-. Concentration Paramete r -" ,..., *-. *: ---' .. .. ' n H (S.U.) e.2\ 'J..c:Ji1- '\.ut4 a.oe, -t.-sz. Co n trol D O (m!!IL) '.t.s *.;.7- +: 1-No n-treated Conductivity "l.'20 --" 3lC\ -. -I --,, -*Alkalinity '* ' ......... ' ... "° \ \o L-.i CaCO;/L) *A,_ --u. ----*Hardne ss ' -'i 4>& -: t1-'j 11112 CaCQ,/Ll .. ' " Temperature (°C) ""l."\.\ < "\.<\. . ., ""\:'\. s 1.."l .\ '1..'-l. lo Initia l Final Initial II F i na l I n i tia l II Final Day (Annlyst identified for each da y , performed pH. D.O. a n d conductivity measurements only.) 3 4 5 6 Ana l yst v---(,L. I).; "'"" ti'-.µ Vv w r . 1 ,: .. . ' . . Co ncen-Parameter . .. r

• trntion *. '. . * .r' .. -. -: *' 6 u. ._ nH (S.U.) :+."i 2..

t-$4 e.l-s-'l.A..! PU°I '.l.4'\ . Contro l DO (mg/L) -:i. 'b IO*--\ "l.-:r 'l-.112 G!o Non-treated Conductivity . -.. s2 v 3 r l 32.4 ;w (µmho s/cm) " . .* *Allrnlinity """ --... -. , (m gCaCO:i/L) -. --'°::::, .. "' ,, *Harclncss -.. ' ' .. , .. 8b ; .. .. . (mg CaC0 1/L) -. . .,_, *Temperature (°C) -,.,\. b ""\-'-\ \.'-\.lo .. "':> ""'"'* \ &..'-\. ') "'\..'-\. tl.!.'-1 Initial Fi n ni In it i a l Finni Initial Fi n al I nitial Fi n al *Te mperatures pcrfom1ed nt th e time oftest initiation. renew al or tenmnalton by the analyst 1dent 1 fi ed in the Dally Renewal 1nromiat1on fable located on Page I. Alk a linity , hardness and total residual chlor i ne pcrfo nned by the onalyst identified on the b en ch sheet specific for each analysis and transcrib ed t o th is bench sheet by:-*. Page 35 of94 SOP A T20 -Exhibit AT 20.3 , rev ision 11-01-14 . ' I Envlronme.n t al Solution)., In c. C oncc11tmtiu11 11nrumc t rr !ull IS lJ l 00 (mi!/Ll Co nlrol , C onduc l ivit y (1 1mho s/cm) No n*lrc:ttcd t\ll mlini t v (mgn_ C:i C OJ) ll nrdnc s s (1111!/L C:1 C O,} Tc 1 n 1>er: uur e ("CJ 11111<;;11 1 no 0 11l rol, ConductM t v (11mho s/cml V-tr ence!I t\lknlinily (mf!/L C:lCO,) Hardne ss (nt t:/L C uCO ,) T em uer: llure (°C) nll ("Ill 10.7% Co nductivil\' (1 rn1 h o s/c 111) Te m11r r: llure ('C) 11111 !SU) 2 1.J o/.. Co nd uc l ivi t\' (µn1ho s/c111) Te111 1 1ern 1ur e (" C) 11>11 (S U) DO(mi!/L) 42.8"/., Co nrluccivil v (ua1ho s/cml Temoeralurc t" C) 11 11 (S UI DO (nll.!.IL) Co nduccivic v (11a1ho s/cm) Tcnm<ru l urc ('Cl n il rS U I Co n ducli v il1* (11*1 h os/c 111l 100% Alk a li nit y (m g/I , CnCO,) ll nr1lnes5 (m glL CnC O J) *Total n csidun l C h lorine 1 111 1!/L Tcmpcra lu rc ( Cl l u ll (SU l DO (m11/t.) C on dur1 iv it v lua1h o s/rn1\ 100% lnt nk< Alk al inity (mgll. CnCO,) ll:m lri css (m gfL C11CO,) *Tomi ll csiduol Chlo rine (m!!.IL Tcmueru cur c ( Cl TVA I Seq u oyah Nuclear P l ant , O ut fa ll 1 0 1 , UV-treated Ma 02-09 , 2017 O n*O lnitiu l 8.2 1 7.9 321 6 1 88 24.7 8.1 9 7.9 322 61 88 24 7 8 18 7.9 309 2 48 8.1 6 79 29 1 2 4.8 8.1 1 1 7.9 260 2<1.8 8.1 3 8.0 1 8 6 1-1.9 8.1 5 8.0 1 60 5 9 M <0.1 0 2 5.0 8.17 8.1 1 63 6 1 64 <0.1 0 24.9 pro m eills Chronic W h o l e E fflu e n t Tox i c i t y Te t EPA-82 1-R-02-0 1 3, Met h od 1 000.0 Da il y C h e mi ca l A n alyses Du l' I O u\1 2 O n v 3 D:tv 4 Finul lnillnl Fimtl l nit l nl 1 7 in11I lnitinl Finni lnithtl 7.9 2 8.10 7.66 8.0 8 7.52 8.1 3 7.83 8.08 7 s 7.7 7.2 7.7 6.6 7.7 7.8 7.8 31 8 3 19 3 26 3 1 7 62 6 3 82 8 6 24.8 14.7 2*1 s 24 7 2 4.6 24.6 24.'I 2 4.6 7.9 3 8.06 7.65 8.06 7.69 s.o s 7.8 1 S.10 7.6 7.9 6.8 79 7.2 8.0 7.7 8.2 325 319 326 3 1 2 64 62 80 80 24.6 24.6 24.7 24 8 24.2 24 8 2 4.2 2 1 1.s 7.8 9 8.09 7.6 1 8.07 7 58 8.06 7 74 8.1 2 7.6 7.9 6.7 79 7.1 8.0 77 8.1 3 18 3 06 3 1 0 2 9 5 2 4.9 2 4.7 24.7 2*19 24.4 '.!4.9 24 5 24.') 7.87 8.1 0 7.6 1 8.0 8 7.5 7 8.0 4 7.77 8.1 1 7.6 7.9 6.7 7.9 6.6 8.0 7.7 8.2 3 0 5 29 2 2 96 2 8 3 24.7 24.7 24.7 25.0 2 4.4 2 5.0 25.0 U7 8.1 0 7.61 8.08 7.57 7.98 7.77 8.0'l 7.5 7.9 6.1 8.0 6.5 8.0 7.7 8.1 267 256 261 250 24.9 24.8 24.5 2 5.0 2 4.0 15.0 24.6 25.U 7.85 8.11 7 59 8 09 7.57 7 99 7.77 8.1 0 7.5 79 6 z 8.0 6.7 8.0 7.7 S I 191 1 8 6 193 18 7 2 4.9 24 9 24 7 2 5.0 24.6 2 5. I 24.6 25.U 7.84 8.0 5 7.5 1 8 0 4 7 56 8.0 3 7.7 6 8.0-1 7.5 7.9 6.2 8.0 6.8 8.0 77 8.1 1 65 1 60 1 6 7 1 65 6 5 69 66 66 <O 10 <0.1 0 2<18 2 4.9 2 4.8 2 5.1 2 4.6 2 5.1 2 4 5 25.1 7.85 8.0 5 7.6*1 8 ,03 7.5 3 8.05 7 7 8 8,07 7.6 8.0 6.9 8.0 6.9 8.0 76 7.9 165 1 58 1 68 1 6 1 65 6 7 66 7 0 <U.10 <0.10 24.9 24.9 '.N.5 25.1 2 4 4 2 5.0 2-1.6 25.1 *Nole-ro 1 nl r cs nlu nl ch lo nn c w os per formed o n no n-irenlcd Ou lf nll IOI a nd *a mp ks Finni 7 5 1 1 6.4 24 3 7.5 5 6.5 24.4 7.5*1 66 2 4.6 7.5 6 6.7 24.6 7 54 68 24.6 7.58 6.6 247 7.5 6 6 7 24.5 7.62 66 24 7 Project numll c r" 1?332 Da v s I nit In I 8.1 5 7.7 32 4 2 4 7 8.06 7.9 3 17 24.7 8.09 8.0 30 4 2 t l.8 S.08 8.0 2 97 2 1 1 9 8.09 8.0 263 2 4 9 8 I 193 24.9 8.06 8 0 169 2 5.U 8.07 8 () 1 70 25 0 Dn v 6 final I nitial F inal 7 84 8.1 9 7.4 9 7.6 7.8 6.0 320 24 , 5 24.S 24.4 7.83 8.14 7.57 7.5 7.9 6.2 321 24.4 24.8 2 4.2 7.84 8.1 6 7A9 7.5 7.9 6.:! 305 2*1.4 24.9 2 4.4 7.83 8.17 7.48 7.6 7.9 6.1 '.!9 3 24.3 250 2 4.4 7.84 8 1 8 7.49 7.6 7.9 6.0 '.!56 !4.5 15.U 1 4.2 7 86 8 19 7.49 7.6 7.9 6.0 1 9 1 24.2 2 5.U 2 4.2 1.87 8.1 9 7.4 8 7.6 8.0 S.9 166 24.2 25.1 24.6 7 9 1 8.1 8 7A8 77 7 9 5.9 167 2 4.5 2 5 1 2 4.5 File: sqn101_05 0 21 7 chem.xlsx E n te r ed by: J. Sumner Reviewed by: __ _ l Page I of7 Chr o nic Whol e Ef flu e nt T oxi city Te st (E P A-8 21-R-0 2-0 13 Me th od 1002.0) S p ecies: Ce riodaplmia d uh i a Client: Te n n e s s ee Va ll ev Aut h o ri tv Faci li ty: Seguo v ah N ucl ea r Pl a nt NPDES #: T N 002 6 4 5 0 Proje c t#: Dif u tio 11 p reparat i o n i 11fo r m ati o 11: Dilution prep (%) 10.7 21.4 Efnucnl volume (ml.) 267.5 535 Diluent volume (ml) 2232.5 1965 41.8 1070 1 43 0 Total volume (ml) 2500 2500 I 2500 Tes t o r a11 is m so ur ce i n fo rmati o n: Orounism aoe: < 24-hours old 85.6 100 2140 25 0 0 360 0 2 500 2500 Co u nty: Ha mil to n O u tfall: 1 0 1 Co mm e n ts: Tes t i11 fo rmati o 11: Randomizin n tern lat e color: Date and ti mes o rgan is m s were bom OS*d\.*\"\ TO 0'\()0 ln cubntor number and s helf t--b_cl_we_c_n: _____ __,-,.--.,,.--.,.......-'----....-,...---=--..-

---

t lo c ntion: Culture beard: Daily r e 11 eiva l i nf ormat i on: Day Date Test initiation a n d feeding, MHSW Snmple numbers used renewal a nd feeding, or batc h u sed Outfa ll 101 In take termi n at i on time 0 05-02-17 o\o\*U--t1 l '"\tfS.11\. (J"t..,. \"\OSO \. o..\ I 05-03-17 o-\*'\le*O

& L O"b n.Oi"o\.O"\ 2 05-04-17 f'lF.."\.." o"\*11-* r\ 3 05-05-17 e, \1. OS(]T)

• l \ 4 05-0 6-17 o& 3<;. (J "nosos. n \"\O$oS. \ & 5 05-07-17

\1OS.OS.\1 \& 6 05-08-1 7 l)&1.\ OS*<n.*\"\ \

• l10CS.cS'.

\i -7 05-09-17 ot'l..\ -oS-o\*t'\ Analyst At A-<-j{ }( -}\ i j1 Co11tro l il iformntiou: S um ma r y o f test e n dpoints: Cootr o l-1 Co o tro l-2 Acee l a n ce crite r ia % of Male Adults: 01. c S20% 7-day LCso % Adu l ts having 3' Broods: lCf)1. l0(:)1. 60% su rv i vin g ad ults N O EC % Mortality: 1. S20% LOEC Mean Offs rino Female: 3.2 1 5.0 offspring/fe mal e C h V %CV: < 42.0% lC2s Page 37 of94 SOP AT! I -Ex11ibit ATI 1.2 , revision 11-01-14 l \-----_,.) Page 2 of7 1 S p e ci es: Cer i o dapl111 ia d uhi n C li e nt: T V A I Se quo ya h N ucl ear P l a nt. Ou t fa ll J OI C O NT RO L-1 S ur v ival mu/ R e pr od u ctio n D a ta Da t e: 0 5-02-1 7 Rep li cate number D ay I 2 3 4 5 6 7 8 9 1 0 I Young producctl () 0 0 D 0 0 0 0 0 u Adult mo r tnlity L. '-\._ '-'-'-'--'-'--\_ 2 Yo u n g prod u cetl ("") ("'} a ("\ t'I (') c 0 c 0 Atlult morln l ily \__. '-\__. \._ '-'-'-'-\.,_ \..._ 3 Yo un g p r o du cctl 0 ("\ f"'I 0 (') 0 0 0 0 0 At.lull mortality \.... \..._ \.._. \...-\..__ L. \_ '--\_ \..._ " \'ou n r; prod u ced \.., l..\ s '5 s I..\ s "!:> '5 Ad u lt m orlnli l y L L '-'--\.... \.,_ '--'--L \._ 5 Young prod u ced l.C n \Q \. 'l.... \ 1.. \Q I t> \ \ Adult mortalit y \._ \.._ '-'-"--\._ \.__ '-\.._ "-G Young p roduced n 0 D 0 0 0 0 0 () () Adult mo 1*tnl i ly L '--\_ '-'--'----'-'-L-L. 7 Young p r oducctl "-\I.. \ "?::> ' '5 ,, '\e \., -\ '(.,, Total young produced -z_ Fin n i Adu lt i\lor t nli l v '-'-'--'-'--'--'-'--'--X fo r J'd U rooib ')£.. ")':-. ")C-)<. "Y-')(:.. 7'---I< >::-Norr:: Ad u l1 mort:rhly (l-h\*t', 0

• dc:3d). SB* sph1 brood (ttr.gle-brood s pl ll bcm'ttn 1 wo CO* cnny O\'Cr (orfspnns O\'tr with ;1duh r nn.s fcr) Co 11 cei 1 tmtio11: % Mortality: I 01. Mean Off sp rin g/Female:

I 3l.'\.... CONC: 10.7% Su r viv al a n d R e p ro du ct i o 11 Da ta Rcp l icnlc number Day I 2 3 "' 5 G 7 8 9 10 I Young p r oduced 0 0 0 D 0 0 0 (') 0 0 A d u l t morlAlity L '-'---\._* \..._ \_ L '--'--L 2 You n g p ro tlu cctl I"\ ("', (\ o* n 0 0 t'i tJ 0 Adul t m or rn l i t y '--'--\..__ L \..._ L L '-'-L J Young p rod u c e d (\ 0 0 0 0 () 0 0 0 u Atlult m nrlnli t y L '-L L L-L L \.__ L L .j Yo u ng produced s .... s '..\ 4 '{ s 5 Ad ult mortn lit y L. L L L L-L L L L L 5 Young pro d uced \ l.. "' \ 1... I\ "'!> \ '-'\ \ '--\\ n .. Adult mortality \.._. '-'-'-'--* L \.__ L L-L G Young producctl 0 I) /) r., {j 0 0 r., 0 () Ad ult m ortnll ty '--\_.. '--\.-L.. '--'-'--'--\..__, 7 Young pro tlu cetl \i l &' I'"\. \e \I \ \o \ l$ I I..\. Total yo u ng pro d ucctl

!> k. 3\ :!> 1..\ 3\ J final Atlulr Mor t ality '-L-. L.. '--'--* L.. '-'--'--Adult nte)tl31it (L .. lrt-c. D ::s dc l d), sn. sphl brood (1m sf c brood spl.11 bc1\\CCR l\\'O dJys). CO-c.lrrv O\'Cf (o sonnc. a.me O\'Cr \\1l .l u t umi: lDM er . h d I d r.) Co 11 ce11 t ratio 11: %M ort ali t y: 07. Mean Offspring/Female

3L.f % Reduction from Control-I: -s. \?. Page 38of94 SOP ATI I -Ex hibit ATI 1.2, rev i sion 11-01-14 Page 3 of 7 Species: Ceriodap/111/(( tlubi(( Client: TV A I Sequoyah Nuclear Plant, Outfall 101 Date: 05-02-17 CONC: 21.4°/0 Survival and Reproduction Data Rcplicntc number Da y I '.! J 4 5 G 7 8 9 10 I Young pr od uced 0 {) n 0 0 n 0i 0 0 0 Adult mortnlity

\._ '-'-L... \..._ '-'-'-'-'-2 Young produced ("'} ,.., 0 n 0 {) CJ 0 0 n Adult mortality \..... L '-'-\_. '-'-\..... '-'-J Young produced ('} n 0 0 0 0 0 0 0 0 Adult mortality L \.... '-'--L '--'-'-\.._ L 4 Young produced '5 '-'-\ '-\ '"-\. s '--\ '"'\ "'\ l...o ' Adult mortality '-'-'-\.._ '-\..... '--'-'---'-5 Young produced \ !t \ ,_ \C \::, \ "1.. \() \\ °' Adult mortality '-'-'-'-'-\_. L '-'--\.._ 6 Young produced 0 0 D 0 0 0 0 0 ri 0 Adu l t mortality L '-'-'-L.. L. \.._ '-'--'-7 Young produced \ °' \., \ -s \ \.o \ C( \ \c, \ '"\ ,, Tomi young produced !>-"'\ b\ "b ,_ :;.\ Finni Adult Mortality \._ \..__ \..... '-'--'-\..... \..... \._ '-N o1 .. *: 1\du l 1 m o rt;,ht y (L

• ll\'C , O
• dt: a d). S O* i p h t brood (s1 11 sf c b roo d split bc 1w cc n l\\'O d J)'S), CO *a rr v (olfJonn c C'.lm c:d o v er 1 1h 3 d ult dunnu tnin s f t"r) Co11ce11rr111iu11:

% Mortal i ty: 01. Mean Off s pring/Female

% Redu c ti o n from Control-I: --i:1't CONC: 42.8% Survival mu! Reprodu ct ion Data Rcnlicntc number Da y I 2 J 4 5 6 7 8 9 JO I Young produced 0 0 0 n () 0 (') 0 (') () Adult mortality L '-L L L L L L L L 2 Yo u ng produced 0 ("'\ f""I 0 t\ 0 a 0 Cl Adult morrnlity \._ \....._ L \._ \....._ \_ \_ \..__ \,.._ '-3 Young produced 0 0 t"\ 0 0 0 0 n 0 () Adult mor t:ilily '-L-L L L L L L L \.._ 4 Young produced 5 s s "'\ s .... l:... c_ Adult mor rnlit y '-L. '-'-\..... '-'--'-'--5 Young produced ' ""l.. \ "'l. \ ""l.. \ '-\ \ t> -\ \ '?:> \ "l.. Adult mortality L \_ L L \.......-'--. '-\..... '-\..._. 6 Young produced 0 0 i\ 0 0 0 a ('\ 0 Ad u lt mortnlity \_ \..-L \.._ \....... L L L 1.... L. 7 Young prod u ced l '\ \8 'l...C \S \$ 't:\ '\c. \ '\ \ -s Total y oung produced o\ 'OC> '"'\ Finni Adull i\lortnlity L \...... '--..__ '-'--.* '-'-'-'-N a u: 1\d uh m oms 1 i 1 y (L

• fh*c. D
• dr J d), SB* spG 1 bro o d (.s 1n_.;lc brood 5p lu bc 1 wc-cn 1 wo d l)'i}, CO* c urv (offs p n n i:: co mrd o v er wn b a du ll dun n 12. L r:1 rufcr) Co 11 ce11trntio11

% Mor1ali1 y: 01. Mean Offsp ri ng/F e male:

% Reduct i on from Control-I: - Page 39 of94 SOP ATI I -E x hibit AT! 1.2 , r evision 11-01-1 4 \-----_,, c...1n.NN 1'U l rut lnqklv: l.:l*L a...:.. Pa g e 4 of 7 S p e ci es: Cerio dap!tuia tlu b itt C li e n t: TVA I Sc qu ovah N u c l ear Pla n t , Ou tfall 101 D ate: 05-02-17 CONC: 8 5.6% S ur v ival and R e produ c tion D ata l{en l ica t c n umber Da y I 2 3 4 5 6 7 8 9 10 I You n g pro du ced [) 0 CJ 0 0 (\ 0 l°'I a 0 Ad ult mortalit y L L '-L \_ '--'---'-'--'-2 You n g p r o duc ed 0 () A {) (\ 0 (') 0 0 n Ad ult mor t a li ty \ '--L \_ '--\._ '-\_ '-.__ 3 You n g pr o du ced fl 0 0 0 0 0 a 0 0 0 Adult mortality L-'-'--\_ '-L L '-\..... \.. " Youn g pr o d uced '-5 -::. '° '-\ l.. $ 'S \..\. A dult morta l ity '--'-'-\_ \_ \_ \....... '--'-'--5 Young pro d u c ed \ '"L \C..\ \\ \ '°';:) l\ A dult morta l it y '--L \...... '--L \__. '---'-\....... \._ 6 Yo u ng prod uc ed 0 0 () 0 0 0 0 c 0 0 A du lt m o r talit y L '-'--L L. L-L-\..... \,_, L.. 7 Yo un g produced \ &. \ '\ l\ 1..0 \ .i. 1..o l\ To t a l y o un g pro du ce d !:>"""\ Fi na l A d u l t J\'io rt a lil)* '-'-'-.__ '--'-'-'--'---'--Not (: A d uh m oni h t y (L

• l wc, 0 *dead). SB =-sp l ll broocJ {sm.£,lc b foOd s pi n b tl\ ... -ce n c w o CO s cu h d I r v O\'c r (o ffs o n ntt crned C\'ct wu a u l d u nnl! t ra n s er). Co 11 ce11t ra tion: % M ortali t y: n1 M co n OfTsp rin g/F ema l e:

% R e du c t i on from C ontrol-I : -'\S. \"l. C O N C: 100% S ur v i v al a 11d R e pr od u ctio11 D a t a Rco l icn t c nu m ber Da y I 2 3 4 5 6 7 8 9 10 I Y o un g pr od u ce d a ('> (\ n 0 () (') ('., (\ 0 A d u l t m ort ali t y '--L L '--L L L '-\_ L.. 2 Y oung pro du ced () 0 0 0 0 fl (\ 0 (""\ (1 Adult mor t a li t y *\...... '-'--\..._ '--'-\...._ '-L \..... 3 Yo u n g p rodu ced n 0 0 0 0 0 0 0 0 0 A dult morta lit y L. L-L L L L L L.. L L -I Y oung [Jro d uccd '-\. s .s l. s s Co '-\. Ad ul t mo r tn l it y '-'-'--"-L L \... \._... '--5 Yo un g prod u ced l'-\ ' ... l \ ,_ \'-\ \"b -\ \\ '""L J 1-Ad u lt m ortn li l)* .,, '-'-'-'-'-'-'-L.-'-6 Yo u ng pr o duc e d 0 a () 0 () 0 () 0 a ('"'\ Adu lt m or t a l i t y \__.. L L l--L \.. L.. L \...... 7 Young pr od u ced i..\ \""\ \i '2 10 L "\ l '\ 1...0 .., Total y o u a g proiluced 31 3'\ 3'\ Fina l A dult Mor t n l ity .__ '--\._.. \...__. \,_ '-'--'--'---\.._ NOi i*: Adult mon al ll y (L 11 lwc , D. SD .... split brood {t in!'J C bro o d spltr bc 1 wccn l WO d.iys:). co -Q n O\'Cf {offs'2 ri rut am c d o v t'.r with d urinc. l ransfcr l Co11c e 111ra t io11: % M ort al i t y: 61. M e an O ffsp rin g/Fema l e: % R ed uc tion fr om C o ntrol-I: -\\.'\ T. S OP A Tl I -Ex h i bit A T I 1.2, r ev i sio n 11-0 1-1 4 Page 40 of94 l Page 5 of? Species: Cerio dnpl111ia dubia Client; TVA I Sequoyah N ucle a r Plant, Ou tfall 10 1 Oa te: 05-02-17 CONTR O L-2 Survival and Reproduction Data Replicate numb er Day I 2 3 4 5 6 7 B 9 10 I Young produced (") 0 0 () () n 0 0 0 0 Adult mortnlit y '-'-'-'--L L L. '-'-'--2 Young produced 0 0 0 0 0 () a () 0 n Adult morta lit y '-'--'-"-\.._ '-'-'-'-3 Young produced 0 0 D (J (', 0 (') 0 c n Adult mortality L \..._ L-'-'-'-L-L_ L L... .j \'oung prod u ced w.. "-{ "-\ "' "'-\ "-:\ '-\ s "-\ Adull mortnlily '-"-\._ '-L '--'-'-\,_ 5 Young pr.oduccd l '-\ "'\... \\ -\0 \0 \0 \\ Adult mortnlit y "-\._ "'-\..._ '--'--'-\.... '--._ G Young pro du ced 0 () 0 0 ('\ (j C) 0 () () Adult mort:tlit y \,_ \.._ L \..... '--\..._... '-L L 7 Young p r o duced \""\ \S \S 1'-1, \& I \o Total young produced :-,\ "!> '"l.. ':!> "2. Finni Adult Mortality \..... \.... '-\_ '-'-\..... '-\.... '-X for J'" l.lro o d s !'-')(_ /<--,c..... "><.. 7<--X-i\'01,*: Adt1lr D'IOn 3hl y (L *hv c. 0

• SO""' sphl brood (s m sle brood spli l between two d:r.y s). CO-any O\'c.r (offspnny earned o"c.r '"h ;iduh dunns tnn.sfer). Co11ce11rrntio 11: % Mortality: I (")1. Mean Offspring/Fema l e: I CONC: 1 00% Intake Survival and Reprod11ctio11 Data Rcnlicatc n umber Dny I 2 3 4 5 G 7 8 9 JO I Young produced 0 0 0 0 0 0 0 D 0 l\ Al.lull mortnlity L '--L L '-'-'--'-'-L '.? \'oung prod u ced ("') n A n ("") (') 0 0 0 () Adult mortnlity L. '-L-\.._ '-L L '-\...... 3 You n g produced D () 0 0 c () 0 0 n G Adult mort:1li1y L L L L '-L L \.._ '-L 4 Young produced I.,. l.o s "' '--\ '4. s s Adult mortnlity

'-..__ '--'-'--....__ L '-\.._ I..._ 5 \'oung produced ' "?:> I\ -\. '"L "L.. \ 1... l '-l lL.. \ \ Adull morrnlity L '-'-\.._ \__... '--'-\_... \.... G Young pro du ced u () {) () 0 0 0 0 0 0 Adult mortality '--L '-L L L L L-\.... L 7 Young pro du ced \ ,.., l\ \ C\ \ \ '\ \cs, 1 \o' no Tolnl you11g produced 1:,1.. Finni Mortality \..... '--'-\.._ \_ L. '--L \,,_ .\'n1c: 1\duh mon.;1J ity (L z Jive. O

• dcd), SO* 1pl11 brood bm£IC brood 1pht between two days). CO* C"1m' over O\'Cf wr l h oiduh dunne irlrW"cr)

Co11ce 11rrntio11

% Mortality: 01. Mean OITspringlfemalc

% Redu ction from C o ntrol-2: -'\.$1. SOP AT I I -Exhibit ATI 1.2 , revision 1 1-01-14 Page 41of94 (Q CD "> 0 .... co Emiro nM t n t" I T0t.Ung 5olu1Jon'lo. Inc. C o n l.-o l-1 ll uy I 2 J I 0 0 0 l 0 0 0 3 0 0 0 J J 6 J s 1 0 II 1 0 6 0 0 0 7 1 6 16 14 T o t ol JO JJ 28 I0.7% ll") I 2 J I 0 Q 0 ? 0 0 0 3 0 0 0 J 5 .1 s s 12 l<I 1 2 6 0 0 0 7 17 18 14 Tom i 3J J 6 31 2 1.4% U oy 1 2 J I 0 0 0 ? II 0 0 l 0 0 0 J s (1 4 5 I J 1:; 11 6 0 0 0 7 19 17 15 Tolul 37 36 JI 42.8% U ny I 2 J I 0 0 0 2 0 0 0 J 0 0 0 J J s 5 5 12 13 12 6 0 0 0 7 19 I ll 20 Tol*I JS J6 J7 Il l' 1licu l l' u u m lJcr J s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 s 5 5 J 12 11 13 1 0 0 0 0 0 1 3 1 5 17 16 JO 32 35 J O Ht111ic:11r u umlJ'-'r J s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 3 ,j .j II 1 3 l'I 12 0 0 0 0 18 1 7 1 3 1 6 JJ J3 JJ J? numbtr J 5 6 7 0 0 0 0 u 0 0 0 0 0 0 _g 4 4 5 .j 1:; IO 1 3 12 0 0 0 I) 16 1 8 1 6 15 J J 32 JJ JI )licn l l' n 11 1nbcr J 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 4 4 5 12 1 4 10 1 4 0 0 0 0 1 5 1 5 I Q 16 J2 JJ JJ JS T V A I Seq uo ya h N ucl ea r Pl a nt , Outfall IO I May 02-09 , 2 0 1 7 Ve rifi c a ti on of Ce ri n t!apf111 ia Rep r odu c tion To ta l s 85.6% 8 9 10 To t nl D uy I ? J 0 0 0 0 I 0 0 0 0 0 0 0 ? 0 0 0 u 0 0 0 J 0 0 0 5 l s 46 4 6 s s 1 0 13 ti 112 ;; 13 12 14 0 0 0 0 G 0 0 u I? 1 4 16 ISJ 7 IS iK 15 J2 JO .)2 Jll 1'0101 J7 JS 34 100% s 9 1 0 Toll11 00) I 2 J 0 0 0 0 I 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 J u 0 0 4 5 5 *14 J 4 s 5 2 II 12 123 5 1 3 1.1 14 0 0 0 0 6 0 0 0 <) I) IJ 161 7 21 1 7 18 J; JI JI J 28 38 J6 37 Co ntro l-2 8 ? 1 0 Tomi Dn y I 2 J 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 *I .j 6 4(1 4 4 4 4 10 1 1 IJ 121 5 12 12 13 u 0 0 0 6 0 0 0 1 9 1 7 17 IC.9 7 1 7 15 IS J.) .ll 3 7 JJ 6 Tot n l 33 31 )2 !O il% I nt ak e 8 9 1 0 Toto I O*y I 2 .) 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 J 0 0 0 6 5 s 48 4 6 6 s 1 3 12 13 12 5 5 13 I I 14 0 0 0 0 , , 0 u u 1 5 19 IS 1 71 1 19 17 17 JJ J G JJ J44 Totnl J 8 J6 lt coll e1 11c num b et Toto! 4 5 6 7 K 9 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 4 6 s s 5 4 5 1 IJ I I IJ 13 13 14 II 1 2 7 0 0 o I o 0 0 0 0 1 9 1 7 19 I 20 IS lO 17 18 1 .)8 n JS I 38 J6 J9 J? JS9 n cullnk 110mbt!r T o mi 4 5 6 7 8 9 10 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 0 0 0 0 0 0 0 6 s 6 (> s 6 .1 n 12 1 4 1 3 l*I 1 1 I! 11 12 9 0 0 0 0 0 0 0 0 IS 18 20 19 19 w 17 1 87 36 37 39 39 JS 38 .l3 3611 ll cn licntc num)er T o 1ol J s 6 7 8 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 6 s 4 4 4 s 4 J 4 13 I I 12 10 10 1 0 I I 11*1 0 0 u 0 0 () 0 0 1 5 1 8 15 14 IS 1 5 I C. 15 8 JJ J 4 JI ?8 J2 JO JI 316 H t11 1i c t llc num b rr Totnl J 5 6 7 8 9 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 6 4 J 4 5 5 s so 12 12 12 14 1 2 IJ 1 1 124 u l1 0 u 0 0 0 0 19 1 9 15 1 9 15 lb 1 6 172 J7 JS JI 37 J? 34 32 34 6 ..f ....... -* -r Environm e ntal T e st i ng So lu tl on s, In c. Concc 11 l rn tion (%) C()ut ro l-I 111.7% 21.4% 85.6% 1 00% '0 111 ro l -2 I UO% l111 akc O utfoll 1 0 1: llu1111 e t1'

• i\I 'D v: 1 luc; l'M S D: Int *kc: llu 11 11 c 11's l'M**n: I 30 \4 37 35 37 3 33 38 2 3 33 18 3r , J I 36 31 36 3 7 35 34 36 37 31 32 J4 J6 2.1 1 3 6.8 1.663 5.3 TVA I Sequoyah Nucl e ar Plant , Outfall 101 May 02-09 , 2017 C hr o nic Whol e E fflu e nt Toxici ty T es t (EP A-821-R-0 2-013 , Method 100 2.0) Ce riodup/111i11 dubia Chronic Whol e E fflu e nt Toxicity Te s t E PA-821-R-02-013 , Method I002.0 Qu a li ty C ont ro l Ve rific a tion of Data E n t r y, C alcul a ti o n s, and S t*1ti s tical A nal y cs rr ojcr t n u mb e r: ___________ 12_J_J_2 R e ul icn t c num ber Sun r i\'n l ,\vcrngc r eprod u c t ion Cot ffiri tr H or f'rrcrnl rrtlnc 1 ion fr om (%} (orr, prini:lfcrnnle) v" r i:uio n ('Y*) 1 moh."4.I ron1rob (*!.) s 6 7 8 9 10 30 J2 35 30 32 30 32 JOO 3 1.2 GA No t ap pli ru b le 3J JJ 3 1 '2 J5 I 31 1 00 32.11 5.7 *5.1 33 32 34 J I 33 32 37 JOO 33.6 6.9 -7.7 32 JJ 33 35 J4 36 33 1 00 3H 4.8 -10.J 38 J_ 38 38 36 39 32 JOO 35.9 7.1 -I S.I 36 37 39 39 35 38 33 1 00 36.8 S.I -1 7.9 34 34 31 28 32 30 31 100 Jl.6 5.8 No t " lll l li c:i bl c 37 r JI '7 32 34 32 I OU JM 7.0 -9.S MSD = Minimum S i g n ilica nt Diflere n cc P M D"' Perce nt Minimum ig n ificant D ifference PM Dis 1.1 measu r e o ft est precision The PMSD 15 thc nnmmu111 percent d 1 1Tc rc ncc be t wee n lh c co n trol and lrcmment th at con be d ec l ared stu11st1cn ll s 1 g nilicant in a whok c lllucnt to x ic1t y test. Lower l'MSD bound dc tc n n111cd by USEP/\ ( 10 11' pcrccntlk)

= 13% Upper PM D bou nd dclcnnin c d by SE I'/\ (90 11' = 47% L O\ er und upper l'MSI) bo und s were determined fr om the I 0 th nnd 90th pcrccn ll lc. rcsp..'C t1 vcly. or PMSD da t u from E P/\'s W ET !ntcrl a boruto ry Variabi l ity tud y(USEP/\. 2001a. USEI'/\. 2001b) llSEPA 2 I ll. 2rHll I , Fi 11:1I Report l11 1crl11bom t m y V:1rinb1hl r Study r E P A ShC1n-tcrn1 Chro n i c mid A cu te Whok Efll ucn l Tox i cit y Method s. V tilumcs I and E l'A-821-13-01-004 nm J 05 LI , Enviro nm c mul P rot e ct i on Agenc y, incmnati. M.

S1a11 Date. 51212017 TV A I Sequoyah Nuclear Plant, Outfall 101 May 02-09, 2017 Statis tical Analy ses Ceriod:.pnnia Survival and Reproduction Test-Roproduction Test ID: C O FRCR Sarnple ID: TVA I SQN Ou L rau 101 End Da l e: 519/2017 Lab 10. ETS-Envi r. Tesling Sol Sample lype* OMR-Oischarge Monitoring Repon Sa mp le Date May 2017 Pro ocol. Fll\iCHR-EPA -821-R-02-013 Test Spec i es: CD-Cenoaaphnia dub ia Comments: Non-L r ealed C one*% 1 2 3 4 5 6 1 8 9 10 Co n l rol-1 30.000 33.000 28.000 30.000 32 000 35.000 30.000 32.000 30.000 32.000 Co .... i rot-2 33.000 31.000 32.000 34.000 3 4.000 31 000 28.000 32 000 30.000 3 1.000 10.7 34.000 36.000 31.000 33.000 31 000 32.000 35.000 31 000 31.000 21.4 37.000 36.000 31.000 33.000 32.000 34 000 31 000 33.000 32.000 37.000 42.6 35.000 36.000 37.000 3 2.000 33.000 33 000 35.000 34.000 36.000 33.000 85.6 37.000 35.000 34.000 38.000 32.000 38.000 38.000 36.000 39.00 0 32.000 10 0 38.000 36.000 37 000 3 6.000 37.000 39.000 39.000 35.000 38.000 33.000 I n take 38.000 3 4.000 36.000 37.000 35.000 31.000 37.000 32.000 34.000 32.000 TrQnsf orm: Unt f'ans formed 1-Tailod Isotonic Cone**/o Moan N-Moan Moan M i n M:>x N t*Stat Cr l tlcal MSO Conlro l-1 31.200 0.9873 31.200 28.000 35.000 6.375 10 34. 117 Control-2 31.600 1 0000 31.600 2 8.000 34.000 5.816 10 10.7 32 800 1.0360 32 800 31.000 36.000 5.713 10 -1.732 2.287 2. 113 34.117 2 1.4 33 , 600 1.0633 33.600 31.000 37 000 6.902 10 -2.598 2.287 2.113 34.117 42.6 34.400 1.0866 34.400 32.000 37.000 4.766 10 -3.463 2.287 2.1'3 34.11 7 85.6 35.900 1.1361 35.900 32.000 39 000 7 126 10 -5.087 2.287 2.113 34. 117 100 36.600 1 1646 36.800 33.000 39000 5.092 10 -G.061 2.267 2.113 34 117 I ntake 34.600 1 , 0949 34.600 31.000 38 , 000 6.974 10 Auxilia!)'. Te s ts St:ttistlc Critic-a l Skow Kolmogorov 0 Test lndicales normal d is tribut ion (p > 0.01) 0.74361 1.035 -0.0134 Bart lett's Test Indicates equal variances (P = 0.81) 2 263 48 15.0663 The con trot means a r e not dfflerent !e = 0.6 51 0.467 1 2.10092 Test p-1<111 , 0.05) NOEC LO EC ChV TU MS Ou MS Op MSB MSE F-Prob Ounnett's Test 1 00 >100 2 11 279 0.06772 41 9367 4.2685.2 1.0&06 1 Lino;ar lntorpol:>tion (200 Re sa mplos) Point % so IC05 >100 IC10 >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 I C50 >100 <0 10 5 0 N g -" 0 ,, "' c; 0 0 0 0 Page44 of94 95%Cl Skow 1.0 ...-------------., 09 08 0 , 7 0.6 :i: 0.5 0.4 0.3 0.2 0.1. 0.0 * ., 0 ---01 -0.2 ........ 0 50 100 150 Do se o/o Dose*Rosponso Plot "' fD "' "' 0 2 ! "' .£ 1-ta f l, 0.05 l evel of s i gnificance N-Moan 1.0000 1.0000 1.0000 1.0 000 1.0000 1 0000 Kurt *0.8025 df s. TVA I Sequoyah Nuclear Plant, Outfall 101 -Intake Ma y 02-09 , 2017 tatisticaJ Ana l yse Env l ronme11talTe:st..lng Snlutlons. Inc.. C e r i odaphnla Surviva l and Reproduction Test-Reproduction Start Da t e: 5/2/2017 Test ID: Cd F RCR Sample ID: TV A I SO N Out f all 101 -Intake End D at e: 5/912017 Lab ID: ETS-Env1r. Testing Sol Sample Type: DMR-Discharge M onitoring Report Sample Date: 2017 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ce r iodaphnia dub1a Comments: No n-treated Cone-% 1 2 3 4 5 6 Control-1 30.000 33.000 28.000 30.000 32.000 35.000 Control-2 33.000 31.000 32.000 34.000 34 000 31.000 10.7 34.000 36.000 31.000 34.000 33.000 31.000 21.4 37.000 36.0 00 31.000

1£.UUU 34.000 42.8 35.000 36.000 37.000 32.000 33.000 33.000 85.6 37.000 35.000 34.000 38.000 32.000 38.000 1 00 38.000 36.000 37.000 36.000 37 000 39.000 I ntake 38.000 34.0 0 0 36.000 37.000 35.000 31.000 Transfo r m: Un t ransformed Cone-% M e a n N-Mean Mean Min Max Contro l-1 31.200 0.9873 31.200 28.000 35.000 Contro l-2 31 600 1.0000 31.600 28.000 34.000 10.7 32.800 1.0380 32.800 31 000 36.000 21.4 33.600 1.0633 33.600 31.000 37.000 42.8 34.400 1.0886 34.400 32.000 37.000 85.6 35.900 1.136 1 35.900 32.000 39.000 1 00 36.800 1.1646 36.800 33.000 39 ODO Intake 34.600 1.0949 34 600 31.000 38.000 Aux ili ary T e s ts Shapi r o-W il k's Test i ndicates normal d i stribution (p > 0.01) F-T e s t indicates e q ual variances (p = 0.43) The co n trol means are n o t significa n tly dif f e r e n t !p = 0.65) Hypothesis Test (1-taif , 0.05) Hornoscedas t ic l Test indicates no sign i ficant differences T r eatments vs Co n trol-2 CV% 6.375 5.816 5 713 6 902 4.786 7.126 5.092 6.97 4 7 30.000 28.000 32.000 31.000 35.000 38.000 39.000 37.000 N 10 1 0 10 10 10 10 10 10 St a t i stic 0.94304 1.72368 0.4671 8 32.000 32.000 35.000 33.000 34.000 36.000 35.000 32.000 t-Sta t -3.128 MSDu MSDp 1.66325 0.05263 9 30.000 30.000 31.000 32.000 36.000 39.000 38.000 34.000 1-Taifed Critical 1.734 Cr i tical 0.868 6.54109 2 1009 2 MSB 45 Dos e-Respons e P l ot 40 .2 30 -g 25 'C e 20 c. Cl> 0:: 15 10 5 0 g c: 0 u Page 45of94 N .... ": 0 "' N .... 0 (.) "' 0 .,.; 0 co ., ..: .!!! E 1-tail , 0.05 le v el of signffica n ce 10 32.000 31.000 31.000 37.000 33.000 32.000 33.000 32.0 0 0 MSD 1.663 Skew Kurt -0.2144 -0.8437 MSE F-P r ob df 4.6 0.00582 1 , 18 Page 6 o f 7 Species: Ce riodap/111ia dubia D ate: 05-02-17 C l ie n t: TVA I Se guo va h N ucle ar P lan t. O u tfall 101 D ail y C/J e 111 i st r)1: Day (Ana l vst idcmificd for each dav , o c rfor m cd oH , 0.0. an d 1.:u11du1.:

ti vitv 1 11ea5u1e1ne111s only.) 0 I 2 A nal y st 1'-1 J-.-., tlV ct-' J-Con centra ti on Pn ra me t cr -----. . . . , < * , -* ..... . -pH (S.U.} A.21 '3 02. A.lb 01"h e..oe DO (m!!IL} 4-q '+..0 1-.f- '+.e Co nduct ivity $Z.\ 32.8 .*. j(q ' (i tm h os/cm) ---* .._ *-.I ' .. C O 'TR OL

• A ll m l i nil y : -*-

, ,. .-(m g CaC0 1/L) lo\ * ..I \o1.. * .I:':." .... , -*Har d n c s *--; -.. .) a1-, CnCO;I L) -. "'Temp e ra t ur e (°C} '"l.'-\. lo '"\.. "\ . "\ L...\..J "\S. \ pH (S.U.) 0.0\ All P'll'\(') DO (mg/L) t>rq ':\-.0 10.7% C ondn cl iv i t y __;01 2o'-( -. (i m1h os/cm) " ... . . . *Te mper atu re (°C} "b\*\ "¥... \ -i,. .. \ .\ -i."\. \c;. * .,_ oH CS.U.l .a,q e>.01 A.\2. /l.QG1 '1R1-DO (m!?/L} 'l.a "")..£.\ '*l.0 21.4% Co ndu ctivity -Aq\ 2qo 1;; (µm h os/cm) -_ c T cmncrnturc (°C) '-'\*\ \>\."'\ "'\..'\. '\ &.'-\. '\ '"\S."1 ... D H (S.U.) e.1e e,.")(') '-".l'2. ;iJ"ID 8.t¥'J DO (me.IL) '.}.q -':\-.e 4 2.8% Co ndn c ti"i!Y itl4'5 *, (111nhos/cm) *.\ -29./) --.. " T emp era tur e (°C) "'\.'\-1 "?.'-\* 1.$. 0 1JH (S.U.) 9.\1. Pion e.02. *>-00 ',\_qg DO (m!?/L} 1=l. q *+.C\ 't.q 8 5.6% Con d uc ti vity -.... -....-.. ; *** -CC*: " (itn ih os/cm) l*>lP I* lOO \61-. -,: .' -.. "T emper at u re (°C) "1.."\* \ L"\-cf ,,4. '\ I..'-\. f "'\.'V\ D H (S.U.) e.1z.. e.111 A,l) l E'J.DS DO (m g/L) a.o e.o qft 4t\ PJIJ C onducti vity t66 { -,,,,'.'. (11mhos/c111) \ ltl'3 lee? r-* --. .... -,:._

• A lk a lini ty *: .. .. -1 0 0% (mgCaCO.i!L) S'\ ' -*Hn rtln c s --. (mg CnCO ,I L) 'b-i.. \o\o -. --*TR chlo rine (mg/L) <o. lO \ "!'-. -. -<. O* lO

.. -:,... *T e mpera t ure (°C) l "l.S.\ L.'-\ * ., "\..'{. '\ -,s.o 1.'t. '1 pH (S.U.) E3.n2-e>.oo A..O\ e.oco DO e.1 r+.o, {:!)J) '\Pe C ondu c t i v i t y Ht>6' ,< ' . -, .. *' lll2.. I! (11 111hos/cm) -__. -.. -' 100% *Al k nlin i!y -!' "* '* -' (.\ Inta ke (m g CaCOJLl ' -*.._ . --*ll nrd n ess . J .--.-,.!-lP'-\ . \o\:i (mg CaC03'Ll J -:..... . ,.,. : -* -*T R c hl orine (mg/L) -'.o. lO . ,0.10 --*-* . " T e mp ernt ur e (°C) '"l.4-i l. "l. C\ i."{. e 1.'\ .'t 1S.O l ni t fal F in a l l n i t i nl F in a l Initia l F in al *T empera 1 ures pcrfon n ed at the tim e oftest initiation, renewal or tem1inntion by t he a n alyst identified in the Daily Renewal

• lnfomiatio n table l ocmcd on Page I. Alkalinity , h ardness mid r esidual c 1lorinc perfom1ed by the analyst ide n ti fi ed on the benc h s heet s pec i fic for each anal ysis and transcribed t o this bench 5hcet by: -...,M-T--

Page 46of94 SOP ATI l -Exh i b i t AT! 1.2, rev i s ion l 1-0 1-14 l l ., l Conccn-trati on CONTROL 10.7% 21.4% 42.8% 85.6% 100% !00% lntnk c .. -\ . ' \-.-----ll',....,.,rwl\,fl'UlfHdnqSohlllriftt.IM. Pag e 7 of7 Species: Ce r iotl ap/111 ia dubia te n t: A e auo va 1 uc e ar er TV I s I N I Pl ant. 0 f 11 10 1 ut a ate: --0 OS 02 1 7 O ny (Analys t idcntilicd for each da y __ per[o m 1 e d pH D.O. nnd conductivitv measu r ements o nl y.) 3 4 5 Ann l ys t V'- i>--'I--1)-.J A.../ Jv --Pnrn m etcr -,-.. ., --' :* . * * -; . .' .:f . . ' . .. --'*'"" pH (S.U.) tJ-i o4 P, ,\.)(\ E:?.\Or. DO (mg/L) '"l e..o a.o Conduc t ivity .. -32'--' 02.J...O . 320 .. 311-" -* _j .. -. -*Alknlinity .. ----"x. .. . -. ... ! (m g CaC0 3'L) . -*Hardness u---' &l.. . ' CaCO i/L) *Te mp e rn turc (°C) \.'"" ""t...!\*'\ ""\Y\. ci ,_ "\ . "' "1.-\.* \ \ "\."\.' oH (S.U.) 0.()1.o i.o'+ 'B*\'2-Z\.c:\1-0.IL"'l e.oe B.\":}. DO . ., r:\A Conductivity J . *-B c o -_,\lo (µmhos/cm) -. .. " Te m perat ur e (°C) \.'-\.*'\ \.'S , \ "'\."\.'\ is."\.. '1."\. & 1.'"\*C\ oH(S.U.) * ...a 'iS .04-'6. \ '2. @,o S.1A DO (mg/L) "':3-Pl 8'.o 0.0 '+(:") 0.0 Cond u ctivity ... I : 2, qz. ' aoo 2'10 (umhos/cm) .. *Te m pe:rnt u r c (°C) 'i>\*"'\ -,,S. '\.. -'\.."\.'\ "1..C.. 'c 1..\ . .:\ 1.."l*"\ pH (S.U.) .o<t-J'\,q',}-Q.10 0.<:A 0.10\ DO (m!!I L) ... g 3.0 li'.n 0.0 r:\_O\ 0-0 ':} C1 Co n d uct i\'il y 2<.Q l _,) ., (umhos/cm) ---" Temperat u re (°C) l.'\-'1 '1>\. "'\>\."\ L') .t:i '1.'\*i ""tS.O \..'\,.'\ oH (S.U.) '6.\2 ... 0.10 0.10 8.\'\ DO (mgf L) ':t A PJ..O O.n i?J.u Cond u cti,*it y \ = -, '1 \\'.(7._... \C\W .. : \6".f (11mhos/cm) .. -,_ . " "'Tcm p ernt u rc (°C) 1.J.\.k "\.'). 0 \ \S.Q pH (S.U.) .0$' 1.t:\'2.. AOC\ DO (m!!IL) , .+R. 'l\.")... ')!.. .... e..\ 0.0 e.o e.o Conduct i vity ., -. \1-\ .. 11oo (µmhos/an) " *: . ' -.. . " Alkalin i t y . ---.. '""' ,. , . . -(mg CaCOi/L) * :! :..,1.* -. -' --' ->. .A' *.c .. . " --. " Hardnes s -----.,---"' -*<' I -. .. *. I ' .. , (m!? CaCO;/L) l* ... ) .. -; : l *-ii::*: chlorine (m g/L) -. -... O*lQ --" -.. -* .. :.; ' ... -. *Tempe r nturc (°C) "\)\.t "\.'"\.. "'\.'\.' '1S*1 1..$.o pH (S.U.) B.oel "i! ';)$'" 0.0\0 e.1e DO (m!!IL) <j\.-z... 'J. a 0tJ 0.0 o,_n ei.1 Cond u ctivit y .. ,. r -.. *p }lP 3 l lo) l'}:t .. . , /""IO (11 mhos/cm) -. , 8 -..... _..;,_!""_ -*-*Alkalinity ' ' t.,S , ' _.. . ' (mit CaC O;IL) '"* --j

• Hardne s s : . ...., . . , --r (mg CaCO JI L) . . II f j *-.. .. *TR ch l orine (mg/L) -> -* :>1 .::a.JO --. ., , le *Tcmpernturc

{°C) 1$.0 '"1.Y,J\ 1.':\.!'\ '1.'i .o o.o I nit in I F in a l I n it i al Finn i Initia l F i na l l n itin l *Te mperatures p e rform ed a l t he time of test initiation , re n e w al or tcrminauon by the analyst 1denufied Ill l h e Daily Renewal lnfo nnation tab l e located on Page I. Alkalinity , lmrdncss and tot al rcsiduald\orine p formed by the analyst i d en t ifi ed on t h e b en ch s h eet speci fi c for each analysis and transcribed t o this ben ch sheet by: . ' Page 47 of94 SOP AT I 1 -Exhibit AT! 1.2 , revis ion 11-01-14 6 .1 v-' ., .. 7 '}A .. .. *I" , .. -----.. _,._.,,. ""'\,C,.1.. 9, ,"q ' -, ----'"\C. .o A.o-1 ... '\.S..'\ .. y ,;;: : '# I..\ .'i 0-<XP '3.o '" ' .J _. "1.S.\ 8.0 1 8.0 . . ' .. '!' ' '.;,c . ..:.1..;_ . ' ---"\.'\. \' e.1 1 6'.1 .* ., . " ' -, , ' ; Final Environmental Testing Solutlons , Inc. C onccntnition Parnmctcr lnll !S U) DO (me/Ll Con ductivil\* (11rnh os/cm) ontr o l Altmlini t y (1111:/L CaC O,) llartl11 css (mi:/L C11COJ) Tc11111ernturc c"Cl 1>11 (SU) 00(1111!/Ll 10.7%. C ond u ctil'ill' (umhos ic m) Tcm 1 1crnturc ("C) oil fSll\ DO lmp/L\ 21.4'Y., C o111 lnrli vit1* (umho s l cm) Tcm u cr atu re <C) 11111 (S ll l 00 (1111:/L} 42.8'Yu C o111luc1ivi11

• fo111hu s1 cm) Tc111 1 1 c n11urc (°(.') 11 111 CSlll DO (me/Ll 8!1.6"/., I Con1l 11 cti"i t v lumho slc m) Tc111 1>cn1111rc

("C) nil IS Ul OO (mi:/I.) C o11ducti"it1* (µm ho s 1cm) 100% Alkn l i ni ty (m i:/L C a C OJ) (mr:IL CaCOJ) Tomi llc si dunl C hlorine (m!!IL) Tcmocrntnrc ("C) 11111 (SUI OO (m!!/I ,) Couductivi t v l11mhos 1 cm) 1 00% lnt:ikc Alk.1 li11 it y (mJ1L CnCOJ} ll nr tln rss (mJ:{I. C aCO ,) Tornl ll cs illuul Chlorine (me!L) Tcmnrmture (°Cl TVA I Sequoyah Nuclear Plant, Outfall 101, Non-treated May 02-09 , 2017 Da v o Initial 8 21 7.9 32 1 61 88 24.6 8.18 7.8 309 24.7 8.19 7.8 291 24.7 S.18 7.9 257 24.7 8.17 7.9 186 24 7 8 12 8.0 158 59 62 <0.10 24. 7 8.02 8.1 1 63 59 1\4 <O 10 24.7 Cerio dap/111ia dubia C h ron i c Who l e Effluent Toxicity Test EPA-82 1-R-02-013, Me th od 1002.0 Dail y C h emical Analyses Day I lhv2 D:w3 Finni Initial Fi1rnl Initial Fi n al Initial Fimtl 8 02 8.10 8 00 8 08 797 8.13 8.04 7 8 77 7 9 7 7 7.8 7.7 7.9 328 3 1 9 316 62 82 25.0 24 7 24.9 24.8 25.1 24.8 24.9 8 01 8.1 1 8.00 8.0R 7 97 8.06 8.04 7.9 i.8 7.9 7.8 7.7 7.S 79 3 1 4 304 310 25.1 24 7 24.7 24 9 25.2 24.9 25.1 S.01 8.12 7.99 8.10 7.97 8.09 8 0 4 79 7 8 79 7.S 7.8 7.8 8.0 293 290 292 24.8 2 47 24.9 24.9 25.2 24.9 25.2 s 00 8.1'.l 8 00 8 08 7.98 S.OS 8 04 7.9 7.8 79 7.8 7.S 7.9 S.O 265 256 26 1 24.8 24 s 24.8 24.9 25.0 24 9 24.8 8.00 S.1 0 S.02 8.08 7 98 8.08 8.03 7.9 7.9 7 CJ 7.8 7.Q 7.9 8.1 1 88 187 19 1 2.t.8 24.9 24.8 24.9 24.9 24 9 24.8 7.99 8.09 8.01 8.05 7.98 7 95 8.05 7.9 8.0 7.9 7.9 8.0 7.9 R2 1 63 167 165 63 66 <O 10 25.1 24.9 24.9 25.0 24.9 25.0 24 8 7.99 8.08 8.01 8.06 7 98 8.05 8.05 7.9 8.0 7.8 7.9 7.8 7.9 8.2 165 1 62 1 63 67 66 <O 10 2 4.9 2 4.8 24.9 25.0 25.0 25.0 24 , 9 Dav 4 ln ith1I 8.08 7.8 317 63 86 24.8 8.12 7.9 2 98 24.9 8 12 8.0 286 24.9 &.1 1 8.0 248 8 1 2 182 2D 8.07 8) 1 58 67 65 <O 2'.i.) 8 11 7.9 165 65 63 <0.10 .2-t9 Fi 1 rn l 7.98 8.0 24.9 7 97 8.0 25.2 7.97 8.0 25.0 7.97 80 25.0 7 97 8.0 25 I 7 92 8 I 24.8 8.06 8.0 25 0 l'rojcct numbe r. ______ 12_3_3_2 O: n-5 l ni t inl 8.15 7.7 324 24.7 S.1 0 78 3 1 6 2 4.8 8.1 0 7.S 300 24.8 8.10 7.9 263 24 8 8.10 8.0 1 96 24.8 8.09 8.0 171 24.9 S.10 8.0 177 24.8 Du y 6 Fi11:1 I ln itinl Fin'11 8.09 8 1 9 8.09 8.0 78 7.9 320 25.1 24.8 25 2 S.08 8.17 S.09 8.0 7.9 7.9 303 2.\.9 24.9 25.0 8 08 8.18 8.04 8.0 7.9 so 290 24.9 24 9 2 4 9 8.09 8.1 9 8.05 8.0 7.9 8.0 257 25.0 24.9 24.9 g 10 8 19 8.06 8.0 8.0 8.0 18 7 25 0 25.0 *25 I S.09 8.1 9 8 01 s.o 8.0 8.0 1 63 25.2 15 0 2 4.8 S.1 1 8.1 8 8.11 8.0 8.1 8.1 166 '.!5.0 .25.0 24.K File: sq n101_05 0217chem.x lsx En tered by: JJmne r Reviewed by: Pag e __ _ Pag e_/_ of_( __ Envtro nm e n ta lli !.ting So l u t l on 1, Inc.. Total Residual Ch lorin e (ORION-1977) Meter: MalTix: Water , RL = 0.10 mg.IL Accumet Model AB250 pH/mV/Ion Meter Analyst D a t e analyzed os-*oz.. *I-:/. Ca li bratio11: O.lOmg/L Reference s tnndnrd num b er j)JJJ' M illivolt s (mV) Sf,1. 9 1.00 mg/L /N.!.J' 5* (,, lodide reage n t I JNR 1-97 Acid reagent: /Nt 7?8 mV C hn ngc (suggested rnngc = 26 to 30 mY) 2'1.-:/ No t e: For sa mple s with a re si dual chlorine of > 1.0 mg/L. th e sa mp les musl be di lul cd to be with i n t he calib rati on ran ge. ll ora t or1 1 coutro s tn11 L b I d f art.: R efe rence sta ndard True value (TV) n u mber (mg/L) J "171, 0.50 D r I up 1 cate samp, e prectswu: Sn mpl e Sample ID Sample chnrncte ri st ic s numb er {l..'lf:I. Rik Jn.,, j Duplicate Sam l e 111 ens 11r e m e 11ts: Sample Sample ID number Reaacnt Blank l1t:J::;D I. Q 3 Mensured va lue (MV) %R = MV /TV x JOO (mg!L) (acce p tab l e range: 90 t o 110%) (l).5'16 loet. 2 7a Re s idual chlorine %RPO= {(S -D) /l(S+D)/21} x 10 0 (me/L) s I. 0-000 D l. 0-00L-f Snmple chara cteri st i cs (acceptab l e ran ge=+/- 10%) /'T.f' O&.H.z,.:T Residu a l c hlorin e (m /L) l. O-oo"l l c). 003 O-ooJ o.ooJ L. 0.002 l 0-0t.)} t ().c:J()J 1 o.ouJ No t e: All samp le s were anal yl ed in exc ess o f E PA recommended holding time ( 15 minutes) un i c ss otherwise noted. L b a ora ory c outro sta 11 d I arr.: Reference sta ndard T rue value (TV) Measured value (MV) %R = MV I T\i x 100 number (mg/l) (rng/L) (acccpta blc ran ge= 90 to II 0%) />.J.sJ 0.50 0.c;H JO?*" /(.? Re vi ewed b y I Dmc revit:wt:LI 3*02..11-Page 49 of94 OP C8 -Exhibit CS. I , revision 01-0 l-13 I l I I I I I I I I I I I I I I I I Page C Envlronment-, J Testing Solut i ons , Inc. Tota l Residua l C h lorine (OJUON-1977) Analyst 1.-/-f-J-----., Date analyzed OS'* o 1 ., Meter: Matrix: Water RL"' 0.10 mg/L Accumet Model AB250 pH/mV/lon Meter Iodide reagent: Acid reagent: Calibration: 0.10 mg/L 1.00 m g/L mV Change (su csccd rn 11 gc = 26 10 JO mV . the samples must be dilu ted 10 be w i thin th e ca librati on range. Labo r a t ory control t a11dartl: Reference standard number True value (TV) (mg/L) 0.50 /-(/JD l I -:J-% RS = MV I TV x 100 ample chnracteristics Residunl c hl orine (m /Ll %RPO= f(S

• 0) /l(S+D)/21}

x 100 (11cccpt11b l e rnngc ="' 1 0%) Dup li cate S I a m p. e 111 eas u re 111 e11ts: n m p i e Snmp l e ID number Reagent Blank \1oSDZ.. \) \'JA\Pc!. / I / /--s Lo.l)J"f D L o-ol Z. ,.....;i i----------o.rvzr,. Samp l e characte r istics Residual c h lorine (mg/L) . ,..)"'tJ?.I?' P:dt . .k ( p.§&'1 ('). -/ / / / .A A f1 / I (/ ---,_ 0 '-...J'._, i Note: All sampl s were analyzed in excess or EPA recommended hold m g lime ( 1 S mmutes) unl ess 0 1hcrw1se 1101ed. labora t ory contro l sta n dard: Refere n ce standard True va l ue (TV) number (mg/L) /JJ .J.J' h 11J 0.50 Mea s ured v:iluc (MV) (mg/LI % RS = M V I TV x 100 (acccplllb l e rnn gc

• 90 to 110%) Reviewed b y I JJ Date reviewed ()5. oz A1-Page 50of94 SOP CS -Exhibit C8. I. revi sio n 01-0 1-13 '

EnYlro n me nl af Testing Solutions, In c. P a ge_.....__ Page _I_ o f_'2_ Total Res i ll u al Cll l odue (ORIO -1977) Me t er: A n a l yst .... , 1-1.-* -'J-------., Matrix: W a ler, RL = 0. I 0 m g/L A cc um et Mo d e l AB 250 pH i mV/l on M e t er Date ana l yzed O':>*D"T*/"l reage nt: ___ -l Acid reagent: L--. Calibrati o 11: 0.L O m g/L L O O m g/L mV Change (s u ge.cste tl r: lllJ?C = 2 6 1 0 30 m VJ Refe r en ce s t an dard n umber I }.,J.t J 1'1?-8 /,41./.J' l"t'f'I M i llivolt s (mV) S":/2 *7' foe>). 2 28. fJ No t e: l'or s amples w i th a res idual chlorin e o f> 1.0 m g/L, the s ample s mu s l be dilut ed t o b e w1l111n th e ca hbr a 11011 ran ge. laborato r ,,-co n trol sta n dard: Ref e rence s ta n dard True v alue (TV) number (m g/L) /ij fJ l'-t ;r g 0.50 D I up/i c at e s amp e pr e ci s ion: Sampl e S ample ID Samp l e c h a ra c t e risti cs number ,, *-1 Dupli ca t e Sam l e 111 ea s11r e 111 e 11t s: Sampl e S11 m p l c ID number Rea ent B l ank \ i IT!:> M ea sur e d v a lu e (M V) % RS = MV I T V x 100 (m g/L) (a cce p ta b l e ra n ge= 90 t o 1 1 0%) Q.Q)3 }00-6 .. R es i d u: 1 l c h l o r i ne %RPO= {(S -D) /(( +D)/2 1} x. J OO (m g/LJ s Lo.ooC. D L,,.o ot S n mpl c chnrnctcri s ti c (nccc pl n blc ran g e-+/- 1 0%) -,...., 0 R esi du11I c hlorine (010/L) -o-0 0'$" O* oo z L o -003 0-oo-Z 0-0 17 0-00 l V-O t>} L o -vo* N ot e: A ll s amp l es were a n alyzed i n e:.: ce ss of E PA r ecomme n ded hold i n g time ( 15 m i nutes) u n les s ot h e r w i se n o t ed. Lb I d d a orato ry c out r o sta n ar : R efe renc e *tand a r d T rue va l u e (T V) Meas ur ed va lu e (M ) %R S=M I T V x 1 00 numb er (m g/L) (mg/L) (acce p tab l e rn n gc = 9 0 to 1 10%) I )J r,1 '"'17r8 0.50 ]"1.k//'v R e vi e w e d b y ___ ---l Da t e re vi e wed '----'O:o.....oSo c...*..;:;lM_,_* J,_1...._ _ _. Page 51of94 SOP CS -E xhib i t C S. I , re vision 01-0 1-1 3 ' Page _.....___ £nvlron m e ntall 6tlng Solutkms# h u:. To t a l R es i d u a l C hl or in e (ORION-1 977) Matrix: Water , RL = 0. I 0 rn g/L .----------.M ete r: Accurn et Mod e l AB250 pH/mYnon Me ter Analyst IHJ I Iodide r eagent: I ........_ rt/' I Date analyzed 0 5 . O'-'/* / 3 : Acid reagent: : " 0"<<..:. Calibration: Labora t ory co11tro l stmulnn l: Reference standnrd n u mber D /" I u p. 1 ca t e snmp e prec 1 s10 11: Samp l e Sample ID number \""\oSo'-\.CS L D upli cate s amp e m easure m ents: 0.10 mg/L True v11lue (TV) (mg/L) 0.50 Sample characteristics P Cl-4. h. rP1 J11. t;,,J,?IP.; amp l e ample ID number Rea1?ent Blank 1.00 mg/L Mca ured va l ue (M (rn g/L) m Cha n ge ( u2gcstcd ranJ!c = 26 t o 30 m V) Resid u al chlorine %.RPO= {(S -D) /l(S..,.D}nl} x 100 (m Q/L) (accep t ab le ra n ge=* 10%) s 0.1z.B D o.1'l.(,. /.' ,0 Sample chnractcristics Rcsidu:il c hl o rin e (mii/L) t ,0ScN.6f u lfl l l,n, )/ ,;,A A C>. O<>{, CJ 7 I I I t-1 I I I ri J '-Ql-// 1--v I I No r e: A ll sa mpl es were analyzed in excess of EPA recommended ho ldin g t un e (15 mmutes) unle s s ot h e rwise noted. labora t or y co ntr ol sta n dard: Reference stan dard True value (T ) Men s urcd v:i l ue (MV} % RS=MV /T x LOO n u mber (rng/L) (mg/L) (acceptab l e range =90 to 110%) /;)JJ I '-1??i 0.50 o.48 1 9'-2.jlo. Reviewed b y I J'.I Date reviewed :::: o:'#-rs ............ ---- Page 52of94 SOP CS-Exhibit C8.l , revision 01-01-13 I I I I I P ag e t l ---Page of Env ronmcm t;.I Tes ti n g So l utlon t.. fnc. Total Residual Ch lori ne (ORIO -1977) Met e r: Matr i x: Water, RL = 0.10 m g/L Acc u met Mode l A B 250 pH/m V/l o n Mete r A n aly s t D a te a n a l yze d hS.t>>. *"l-Calibrat i o11: O.JO mg/L Reference s t a ndard number 'NSS1 Milli v olt s (mV) 5 t.00 m g/L r eag. e nt: [IN l-3o/l Ac i d reage n t: ('l rl -=1"8 "-'----"-=------' m V Ch a ng e (S U J?l?CS I Cd ran c: 26 f O 30 m V) N o t e: For samp l es w i th a residual chl or mr. o f > 1.0 m L , th e 5 ampl es m us t be diluted t o ut: wilh i n t h e c libration ran ge. laborato r

• c outrol s tandard: R e ference s t a nd a rd numb e r Du li c ate s am 1/e 'J recisio11: S ampl e S a m p l e ID numb er noso c...o Me...y._ Duplicate Snm 'J/e m e as11r e 111 e 111s: T rue v alu e (f V) {m g.IL) 0.50 S ampl e ch a ra c teri s ti cs Sampl e Sampl e ID number Reaoent Blank M easured val u e (M V) (m g/L) o. Residual chlor i n e (m IL) % RS = MV I TV x I 00 (accept a ble rnngc .. 90 t o 110%) %RPO= {(S -D) /f( +D)/2 1} x 100 (nccc pt n bl c rnn g c =;!; 1 0%) ample c haract e r is ti cs Residunl chlorin e O .c\\p < O.OQ .... b.co-:r < .:> * .QO\ N ote: All s amples \\Cre analyz e d i n e x cess o f E PA recom m en d ed ho l d i n g time (IS minul cs) unl es s o t h e rwise n o t e d. laborator co11trol sta11tlnrll:

Ref e r e nc e st nndnrd number True v alu e (T V) {m g/L) 0.50 Mea s ur e d vn lu e (M V) (m g/L) % R = M V I TV x I 00 (a cce pt a bl e ra n g e* 9 0 lo 110%) q Revi e wed by I j{ D at e re i ewed I I I I Page 53of94 SOP CS -Exh;bit CS. I , "vi,;o, 01-01-_ J l l l l l l l l J J l J J J J G A'.O?,., (;- ' "-1'

• o .* .. 1 t r:* ,, ,,.,..,,..., I* 0 !:: t .: . *. -* L_ ; .-JJ -* _J '..._../ 5 En vfr onmtnt il lTu ti ng 5otu 1i ons.. Inc:. Analyst I \Iv Date analyzed 2-6-.\-')-

--1 Alkalinity (SM 2320 B-1997) Matrix: Water , RL = 1.0 mg CaCO,IL Titrate samples to pH=4.5 S.U. Page 3(p Page ___ of __ _ Time initiated I r I 05 Time completed ===( :12==2-======== Titra11t 11orma/ity anti multiplier tleter111i11atio11: pH of Normnlity Normality (N) pH F actor or Multiplier Deionized Titrant check Begin End Total = (5 ml Nn 2 C0 3 x 0.05)/E = (N x 50000)/ 100 ml snmple water reference standard ml ml ml "'0.25/E =Nx 500 =4.5 s.u. number number (E) (acccp1 a blc rnngc = 0.0180 -0.0220) 6.-+ lNRu'-t l

o. '2.. l2.'-I 12.L. o.o-i.o 5"' 1\..J.2..

tlll(...Wf\N.t..1 -o. -0.'2. :.o. l a oratorv co11tro slam art: Lb I CJI I Reference standnrd True value Samp l e Alkalinity (MV) %RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier (mg CnCO;/L) (a cccptnblc range (mg CnCOJ/L) (ml) ml ml ml = 90 to 110%) I 100 100 12-'*4 Jo.z.. (b.1-(DY llY**I. (/). D r l up. 1cate samp, e prec1s1011: Snmple Alkalinity %RPO= Sample Snmplc ID volum e Begin End Total Multiplier (mg C:iC03'L) {(S

• D) /l(S+D)/21}

x I 00 number (ml) ml ml ml (:icccptablc range=+/- 10%) n .SSw f UO M1.1a lo.z. s 35 '* --.*-* _,.:.";'.: ....... .. .. . ... _, ** !r * *: " . j Dupl i cat e (B) ...l l4.'> act.'1 2.v( J -O'-<"ZeJJ7 Mi . 'k alrtx S!Jt *e recoverv: Reference st:mdnrd Spike vnlue Snmplc Spike alkalinity (A) number (SY) volume Begin End Total Multiplier (mg CaC03/L) (mg C1C03'Ll (ml) ml ml ml I 6D \ L)O oLL'I eLJ Sample :'lll<allnlty (B) Measured s11il c e value (MY) % R -MY I SV x 100 (mg C n COJIL) MY=A-B (:icccpt:tblc rnngc (mg C n COJILJ =75 to 125%) 9; '1 a, Cf ei.in. s l amp e 111eas11reme11ts: Sample volume Begin EPd Total Alkalinity Samole\Buui'ber Sample ID (ml) ml ml ml Multiplier (mi;: C:iCOJ/L) SSLiU 100 '.31. e .; . \..\ *O:i !>5 19 '-1

• I CLJ./'} fn Hsw I q,1.e l;.3 o4-t a.rtA I 1,*1 *i *' lo. "?I 0'1* ,q.11-12> 14d v.o 04*

IY.1 (d.O (t1 I O'+OMn b to. I [4 z.. (J '::4 *8. (o.fT fl 0V:i.SvJ 2.. 'O. *1 C/o 'd.*b.11* (6 ..1 t.l4.o Q.o ' qz.. j 42en : -Reviewed by: Date reviewed: j 6$*0\*\"'1 Page 54of94 SOP C6 -Exhibit C6. I , revision 01-01-13 l l l l l l l I J J J I -Analyst I Ssv Date analyzed :=: 0: 0:.:01==*\=:1:===== Alkalinity (SM 2320 B-1997) Matrix: Water, RL = LO mg CaC03'L Titrate samples to pH= 4.5 S.U. Page 31 S Time initiated I ':::: Time completed

=: l!.L:.,,:

... r: I /"/' f 1trn11t norma 1t11 am 11111 ttp. ter r.etermmnt1011: pH of Normality Normality (N) of pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Nn 2 COJ x 0.05)/E = (N x 50000)/ 100 ml snmp le wate r reference stnndard ml ml ml =0.25/E "" NX 500 =4.SS.U. number number <El (:icccplnblc r.ingc = 0.0180

• 0.0220)

S,..1'$ IS"i:'l-O.Y. 1'2..<Z 12..4 0 .0 'Z..t'.Y2-JO.\ a orn ory co11 o slam an; Reference st:rndnrd True vnlue Sample Alkal ini ty (MY) % RS= MY I TV x 100 number (TV) volume Begin End Totnl Multiplier (mg CaCOJ/L) (ncccplnblc range (mg CnCOJ/L) (ml) ml ml ml = 90 to 110%) ::r \JS'.:> l?S-3 100 100 In o \0. \ }\::11.0 IDl.o 0/.:> D l" I up, rcate samp. e prec1s1011: Sample Alkalinity %RPO= Sample Snmple iD volume Begin End Totnl Multiplier (mg CnCOJ/L) {(S

• DJ /f{S+D)/21}

x 100 number (ml) ml ml ml (ncccptnblc rnngc "': 10%) s ... ' -. .:;. ;. :: '"'-:.: ' " o;.tt-\.A-SSiv0 }II() n.i *21co.\ 3.?, \o. \ 33 .*. ,. ; ... ..J Duplica1e (B) J_ l--D e;.'"' 0'5 ... 1 l1 2.lP.\ zq."+ 3.3 33 M "k atnx spt e recovery: Reference standard Spike.value Sample Spike nl knli nity (A) number (SY) vo l ume Begin End Totnl Multiplier (mg CnCOJ/L) (mg CnCOJ/L) (ml) ml ml ml \'33? 100 "Z.(o.. \ 13.'2. \0.\ 13D Sampl e all<nl n ty (B) Meas ur cu spilce value (MY) % R -MY I SV .x 100 (mg C:iCOJ/L J MV=A-B (ncccp r ablc rnngc (mg CnCOJ/L) =1 5 to 125%) 33 'i1-'1'°:J'.D 0 1c s m11p, e 111ensureme11ts

Sample volume Begin End Total Alknlinity Snmole number Sample ID (ml) ml ml ml Multio li er (mg CaCOJ/L) 06.t)2..r:t m \+.::vJ \O'O IJ..1.5.'S

w. '2. \0.\ lq3 I o.o 1,,0 \.o \ 0505.11-.. In\> \'2...o lo.o \, \ .... --£ .\ -*lJ,-\I A rnHsw uv \ -...... \*b l.lP\.C: V1 I -;,\..\."lb*

Cl f> 2-...... . ..... I lOi> 2.4-.'l 1,.3 L,,4-os.-cn..-\\ J 3 _ ..... '2.1. 2. Lt. I v'2 ', Q$c) \.<fl-I '41>.1 3.1+ ..,, ' l o'l t\ oS<T':> I 2. I L14.() °' 1_'\ 3 I J_ l.lP . n oc; as . l \o 3.\ '.'I.) ----t-3 Reviewed by: Date reviewed: I tn-r1 Page 55 of 94 of-(v*nrt-19 t(... SOP C6 -Exhibit C6. l, revision O 1-01-13 l l l l l *1 I r .I J J I Analyst! Date analyzed DS:;;-;r

• ij Alkalin i ty (SM 2320 B-199 7) Matrix: Water, RL = 1.0 mg CaC0 3/L Tit ra te sa mp les to pH=4.S S.U. Page 3<i Page of 5 Time initiated F

Time c o mpleted S T: I I. r l r 1frm1111orma 1 tv mu , 111 11 ltp 1 er t etermma/1 011: pliOT-r--Normality Normality (N) of pH Factor o r Mu l tiplier Deionized Titrnnt r--.. Rea i n End Total = (5 ml Na 2 C0 3

• 0.05)/E = (N x 5000 0)/ 100 ml samp l e .. water reference st:rndard ml _, =0.25/E =Nx500 "" --Cnccc"}1t:tble.i;ane:c

= 0.0180 -0.0220) =4.5 s.u. number numbe r (E) ...;..050 1o<T Lb l I a ora t ory co 11 t r o s l a m a n: Reference standard True value Sample Alkalinity (MVY % RS = MV I TV x I 00 number (TV) volume Begin End Tota l Multiplier {mg CaC O JJ'L) (acccplnblc rnngc (mg CnCQ3/L) (ml) ml ml ml .. 90 to 110%) 100 100 -"?-. -=r-1 0.1 J O.\ l0'2.. 1 02.-, 0 Oto D r l 11p t c a le sa mv e p r ec r s 1 011: Sample Allmli n ity %RPD= Sample Samp l e ID volume Begin End Total Multiplier (mg CnCO.i/L) l(S -0) /((S+0)/21} x 100 number (ml) ml ml ml range c:f: 1 0%) 8""" eMu f-1 lY"\ s 1**-:..-** *""[!"'-, * ,. :; *r .....

• l-"1 O.SO\.0' l to o 9 1n.) 3'1 ; :: .";. ... ' .. " _;.* Duplica1e (B) J_ .l. D 1'il*r "2.'Z-.v 3 .'\ 3q QS.O .. H:J utnx spL e recovery:

Reference stnndard Spil<ev:i l uc Sample Spike allcnli n ity (A) number (SY) volume Begin End Total Multiplier (mgCnCOJIL) (me. C:iCOJi'L) (ml) ml m l ml 5o JOD 1i.-r *).1::\-9.o l o.I '11 Snmp l e alka li nity (B) Me: 1 s u l'ed spike v:ilue (MY) % R -MY I SY x 100 (mg CnC03'L) MV=A-B (acceptab l e rnngc (me: CaCOJIL) .. 75 to 125%) 3q 52 tc't. o l>j o S I amp e m eas 11r e111e11ts: Sample vo l ume Begin End Total A l kalinity Samole number Sam11 l e ID (ml) m l m l m l Mu l t i plier (me. CaCO.i!L l \\05.lfu. -:r t.Hv fun 2-100 ::l"l .1-;ln_R 3. \ fU.\ t\c S.C'S..\C:.. J .3 _L &>.'J 4.1.Q 32 1 1. 0S 01..o..f Ao.1 , .. D l 'JC::. 5+.o .:SS. I 1.1 l/4) .w.u. \ 1 o S o'-! .ol r 2.. I I 1.3 !< 4) 52 \I b C:..Q\o. b\ l 3 _L J.'L (.o+.) .t.\.'b n o scn.-1-4 T"tc_ 'J _ ()(")\ A-m :.>>':r.v 2.ct LA'\ 1"2.0-l\t . no S(ll...\S Ji.ilS .... s.1. 114) 1 30 * \2C\ \\ O S.<:1 2.* n_ P N\ Mto.1,1 2..q 'r ...i. '\ -\ \1 1.1 0 ftn 1$ Q ........ o.o 8.1 3.\ --\-3-,.,... .. , -z...o -1z:; 110.* ... .... Reviewed by: Date reviewed: J 0 s.-1)1 * .-1 Page 56of94 SOP C6 -Exhibit C6.1 , revision 01-01-13 l l l l l J I _J J J J J 0 c* y* ... , * "' r y;..----*..) *i" 1 ') LSC .)'i -, . I , .. _. ' --t.___../ ). Env l ronmrnlitl Solu\i on s. Inc. Analyst I & Date aaa l yzed Alkalinity (SM 2320 B-1997) Matrix: Water, RL = 1.0 mg CaC0 3/L Titrate samples to pH=4.5 S.U. Page 39 Page 3 of_5 __ Time initiated I':........,_ Time completed r: r l' /' l 1lra11t 11orma 1ty am mu ftp rer' ete rmma/1011: .. ,. Normality Normality (N) pH Factor or Multiplier .---Deionized Titrant cnci:" R .. arn End To fa I (5 mJ N11 1 C0 3 x 0.05)/E (Nx 50000)/ 100 ml sample water reference standard ml m1 0.25/E -Nx 500 -4.5 s.u. number number (E) (ncccplnb1c r: n 1'1110

• 0.0220) -.. Lb I l I a oratory coutro stmu art : Reference standard True value Sample Alkalinity (MV) % RS= MY I TV x 100 number (TV) volume Begin End Total Multiplier (mgCnCO.v'L) (acccplnblc range (mg CaCO.i/L} (ml) ml ml ml =90to110%} "f t::PS \S'S :S 100 100 3.\ 13.1.-j'D.\ Jo.I \oG..

D /' I up, 1cate samp e preCJs1011: Snmple Alknlinity %RPD= Sample Sample ID volume Begin End Totnl Multiplier (mg C:iCO.YL) {(S

• 0) /((S+0)/21}

x 100 number 1V'A (ml) ml ml ml (ncccplnblc 10%) . s l\oD -\'>*>; .. *. ... .. ... ' \ 1 Cf::lS\.1.f f\t.-F OCl 13-'2-21.0 -=1.'if 'l.) In.I . -** *--. *-* l Duplicate (B) j_ Li.) ..\_ D 2. J.o 2.c::r.o lltO 06°0"1-!1-atrtx S/Jl e recovery: Reference st:tndard Spike value Samp l e Spilce allcnlinity (A) number (SV) volume Begin End Tot11I Multiplier (mg CaCO.YL) (mgC:iCO.i/Ll (ml) ml ml ml lSS3 100 ... 50 2.)."> 13.0 :z.) 10.\ '2..1..e c -.... ,.,.,.. Snmple allrnlinity (B) Measured spike valu e (MY) % R "" MY I SV x 100 (mg CnCOJ/L) MV=A-B (acccptnblc rnngc (me CnCO.i/L) =75 to 125%) llPO :2;,., ""'"" ') * " \OC \eo.c"'/o I s nmp. e 111eas11reme11ts: Sample volume Begin End Total Allcnlinity Sample number Samole ID (ml) ml ml ml Multiplier (m!! CnCO.VL) r1 O.S\Th * -fJAAt r-vp 5o 'ti .. ') \'C). \ 3<.. j I Ni I L..IJ,I.\ S.K ,.,_-., l'Z-0 -\It , rnM1w 2.5 l),Q 3.'1 II.+) \loo \' c.s. o-i-<lS QN. c-n ho c.U. So .3 .'I -:}.o ll1. \ , , a. , 6ct \1 \O\ \ +.t> 'l.1 L'L\ I 2. -=i.q 13.0 3 ,\ I">,'\ k '2, \4C\So'S. \\ J 21 13*0 flD.3 .'l, (,-i.-) 1\0So\.ti.J. '1.Jlt S'Qr-> 'Q I 1,.JT l llt*"i 14..1 l) .'l (;). '\ '5G\ \\ 0 S.<P.>*1>\o 2 --1q:i c.,,...\ -Vt Reviewed by: Date reviewed: j -01* \" Page 57 of94 SOP C6 -Exhibit C6. l , revision 01-01-13 l l l l l ] .1 I .. .J _J J J I I _...; Analyst j f?Su Date analyzed ._f-_-_ ... Alkalinity (SM 2320 B-1997) Matrix: Water , RL = 1.0 mg CaCO J/L Titrate samples to pH=4.5 S.U. Page Page !.-{ o f 5 ---Time initiated Time completed I ..__ ______ _, Titraut nor ma lity and multiplier tfeter111i11atio11: Pu v. Norm3lity Normality (N) of pH Factor or Multiplier Deionized Titrnnt check .,_ .. Total (5 m l Na 2 C0 3 x 0.05)/E (N x 50000)/ 100 ml sampl e --.. "' water reference st:ind:ird ml ml lnr- =Nx500 = 4.5 s.u. number number (E) ( p b =0.0180-0.0220) ---.. Lb a oratory contra I t I I Sant art: Reference stand:ird True vnfuc Snmple Alkalinity (MY) % RS= MY I TV x 100 number (TV) volume Begin End Tot:il Multiplier (mg CnC03/L) (ncccplnblc r:ingc (mg CnC03/L) (ml) ml ml ml =90 t o110%) 100 100 =32 IT SS\-I t;j;;:,.o 0/ .. c:i.q 11.,.k tro. I I t>t> D r I up, tcate S ff l11fJ e prec1s1011: Sample Alk:ilinity %RPD= Sample Sample lD volume Begin End Total Multiplier (mg CnC03/L) ((S

• D) /f(S+D)/2)} x 100 number '&../IA (ml) ml ml ml (ncccplnblc range*: JO%) s -. -. ,. . **.: . ... ..... *.*t*.'"*-;:: .. ' no$ ID I 5't> 32..4 10.\ lO 5o 35.v Hg.l. '0.'2.. \0.1 110 Sample allm l inity (B) Mensured spil<e v:iJue (MY) %R=MV/SV.1.JOO (mg CnC03'L) MV=A-B (:icccptablc rnngc (mg CoCO.VL) =75 t o 125%) (;5 nr IOS' Ori:' " I05'JI, -s amp, e 111ens11re111e11ts: Sample volume Begin End Tot:il Alkalinity Samp l e number Sample JD (ml) ml ml ml Multiplier (m2CnCO.i!Ll \10 SQ\.(C) t0 l 01 Uv \ So (),0 2. .'1 2.'l ,.,_, IC.\

l "l OS'S?> :6 I 2. 2. Lr.I .. !-?\ J 3 . \'"\o<":ioS. \1 le.I 't.c; 3.c+ U.\ (t>Cf \\D S C\ .6 4 l..J ASG..-NlCl \>Jl\l\J \ C'+ J.:'55' t'a5 .,3.o l4A lt I .. ,.... -"" , .. , z_ 11:-? .;;>1.1 3.i..os lo5 I 4+.-5_ ('2.\ noS0'5. 3 ......... __ ,., , -- .;J.S.o 3.-1. D-1 \\ OSIR..-OS \ 2.-q.S 11.5 z..o L?o) I ooC Y\ I '2.-l 11.c:;' 15.lo .9.. \ fc..,") 1\00 \\ ! 3 J-15.S ).g I c:T>\--- '\Vo Reviewed by: Date rev i ewed: I O!.* en .1-\J Page 58of94 SOP C6 -Exhibit C6. l, revision 01-01-1 3 l l l l l J J l J Analyst I Sc:i v Date ana l yzed 1--o"'""s- .-o-"'.}-*-lt----1 Alkalinity (SM 2320 B-1997) Matrix: Water, RL = 1.0 mg CaC0 3/L Titrate samples to pH=4.S S.U. Page '4-1 Page 5 of 5 ---.Time initiated Time completed r----.__ _____ __, Ti trant 11ormalit mul 11111/ti lier delermi11afio11: De i onized Titrant water reference = 4.5 S.U. number number L b I l I a oratory contro slam art : Reference standard True value Sample number (TV) volume Begin (mg CnCOJIL) (ml) ml ISS-3 100 100 D /' I up, tcate samp, e prec1s1011: Sampl e Sample Sample ID volume Begin number (ml) ml 1 I Q.507.. ok:> 1::;.r\/1.1 l hlln.cJ \ 1. 35,0 l Dup!Matc (B) ..!-o.o .,, atnx sp1 <e recovery: Reference stnndnrd Spike vnlue Sampl e number (SV) vo lum e Begin (me. CnC03'L) (ml) ml 'f }-.!SS .::<5oo ?._ o.o Snmple allrnlinity (B) (mg CnCOJ/L} 3300 s l amp, e 111ens11re111e11ts: End ml 35.o End ml 4J.'+ End ml 11.S Normality (N) of H 2 SO J = (5 ml Na 2 C0 3 x 0.05)/E =0.25/E pH Fnctor or Multiplier = (N x 50000)1100 ml s ample =Nx500 range* 0.0180 -0.0220) All<alinity (MV) %RS=MV/TVx 100 Total Multiplier (mg CaC03'L) (ncccptnblc range ml = 90to110%) 11> .o fo.1 IOI \o l. o 0 (o Allcnlinity %RPD= Total Multiplier (mg CaC03'L) {(S -0) /((S+D)/21) x 100 ml (acceptable r:in g c = :1: 10%) s ;.**** .;- ... lt .'-+ t<n\IO.l 3z.oO _...,_-..... I *o\ _l D -Ir.

• 33oO 'l:l.o!. .. f>.<;' ... .. )n... Spike alknlinity (A) Totnl Multiplier (mg CnC03/L) ml 11.'3 \t:>.I 5l?oo Measured spike vnlue (MV) % R= MV I SY x 100 MV .. A-B (ncccptnblc rnn g c (me. CnC03'L) =75 to 125%} :<5oo joO .C) 0/o Sample volume Begin End Total Alkalinity Snmple number Sample ID (ml) ml ml ml Multiplier (mg C:iC0 3'L) '\\

<[) hOAd 2-'.2. II.$' lD.4 )O. \ .., 1\osot...o'-l j 3 r:i.q II.'+ 3-z-.oo n os01...<J1 o4c."'\L \ ..71-l..2, '+.o <=io' . noS£1-t.<e> I 2. 2.S.'; 4.'0 '2,Qoo \\ O'S.D\...Cf.;> .l 3 1-\..o F\'()) -Z.Ooo 06,0t.{.1'} I oO t.!U.S lo.'2. IC><:> -S1 L 'b/0-o 1 Rev i ewed by: Date reviewed: I OS-CJ)-1 1 Page 59 of94 SOP C6 -Exhibit C6.l , revision 01-01-1 3 I .1 l l l l l l l _I I J J J l rfPJ ,-f *. : t *r--I ; ..._ I _; _J c_-:,./ Page } Page __ l_of 6 \-------*-* E n v!ronmtnUI Tas dng Solutlons. Inc. Hardness (SM 2340 C-1997) RL = 1.0 mg Analyst I lu Date analyzed t I l -r . r l I 1 ran uorma 11y ant mu tip, 1er t e ermma t011: Titrant Normality check Begin End Total reference stan dard ml ml ml number number (E) 1.tJq,.--=tQ, I NsSI 1-Nl4 l).() \O.o L b I I I a oratory contro stam nn: Reference standard True vnlue Sample numbe r (TY) volume Begin End (mg C:iC03/L) (ml) ml ml \ N$S 1'-1-ef} 40 50 (D.0 \2_o D r I up. 1cate samp, e prec1s1011

Snmple Sample Snmple ID volume Begin End number (ml) ml ml Q4*80. /). .Ssw Ob l2.o l'-t.o .! Duplicnte (B) jvlo M "Ii atnx sp1 *e recovery; Reference standnrd Spike vnluc Sample number (SV) volume Begin End (mg CnCOJIL) (ml) ml ml \JllS.S1'ltH 6D 14.u \*>,u Time initiated

..... -=<.P.__ __ ---1 Time completed ._ .... _Q""'-6l.P""'-=------' Normality (N) of EDTA pH Factor or Multiplier = 0.2JE = (N x 50000)/ 50 ml sa mpl e (:icccpfnblc rnngc = 0.0180-0.0220) =Nx 1000 0.0200 z_o.o Hnrdness (MV) %RS=MV/TVx 100 Totnl Multiplier (mgC:iCO.v'L) (:iccc pr:ib l c rnngc ml = 90 to 110%) -z_o -w.o '-lo \00 01. Hnrdness %RPD= Totnl Multiplier (mg C:1CQ3/L) { S -D} /f{S+D)/2J) x IOO ml '2.,o Zo.o s YO : .. **' j D 40 --41-.0 '-I 2e n-Spike hardness (A) Total Multiplier (mg CnCOJIL) ml zo.o eo Sample hnrclncss (B) Measured spike vnluc (MV) % R= MV I SV x 100 (n1g C n C:OJ/I.) MV=A-B (ncccptnblc rn11gc (mg CnCO.v'L) =75 to 125%} '--10 yo 100. in. s l amp. e 111eas11re111e11ts: Sample volume Begin End Total Hardness Snmole number Snmole ID (ml) ml ml ml MultioJier (mg CnCOJ/L) TV Blank 5D lA I. (should be = 0 111£ CaCO.;/L) l).Q b.O o.o w.o O'-{ 'e:>.c> 2,).() "2.,o r '-JO 6E>W O"l.11a.n mi+sw "'4.ltJ 4.1.tJ Cl2.. b Y.14.11-A J.9./ '{.-;-C/o l')'{.j q.1}8 iA'1.J j.3.-\ 66 O\,/.JltJ.11 A "3.J. '.) J.y 01../ *9.0.11 r.... ' '-41.c -t../.1 ez.. bv (-} 4W '-4i.8 I (.ti I v {b 41.e; O.i.o (2 J (.., 43.4 44:0 ' 12-Note: If >15m l oftitranl is used, sample must be diluted. Reviewed by: Page 60of94 Date reviewed I 6S *o\* t'\ SOP C7 -Exhibit C7.I. revision 01-0 1-1 3 l l l l l l l l l I .I J I J J Analyst I Lf'._, Date analyzed r:1 u* r l t . /' t 1 ra11 11or111a uy a11t 11111 tp, 1er t e ermma t011: Hardness (SM 2340 C-1997) RL = 1.0 mg CaC0 3/L Page 2. ---Page 2-of 3 -------Time i ni t i at e d E 01.t*2-(9p. ---i Time completed Htr..fillf Normality check Begin End Tot a l Norma l ity (N) ofEDTA pH Factor or Mult ipli er reference standard "" I .1 0.2/E =(N 50000)/ 50 ml sa mpl e number number (E) (:tcccptnb1e =Nx 1000 v-L b I l I a oratory contro slam a n: Reference standard True value Snmple Hardness (MV) %RS=MV/T Vx 100 number (TV) volume Begin End Totnl Multiplier (mg CnCOJIL) (n cccptnblc range (mg C:iC03/L) (ml) ml ml ml = 90 to 110%) \'-it)"} 40 50 c..\"10 z_o w.0 YO l c:xJ. ({), D t: l up. 1cate sm,,-o. e prec1s1011: Sampl e Hardness %RPD= S ample Snmple ID volume Begin End Totnl Multiplier (m g CnCOJ/L) {(S-0) /((S+D)/21} x 100 number (ml) ml ml ml Q\)Ot)Jn D 60 13.1 IY.1 1.0 s 't<J .. -io.o .. Duplicate (JU .J l <..\. t 15.1 1.0 1 D --1.1 't-<) /; atnx spi <e recoveri 1: R efe rence st:mdard Spik e vnlue Sampl e Spike hnrdness (A) n umb<?r (SV) volume B<?gin End Total Multiplier {mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml \ '4D 6U \ ll.I l1-o 2. .t1 'ZD-0 66 Snmpl<? hnrdncss (B) Mc.1surcd spike value (MV) % R = MV I SV x 100 (mg CnCOJIL) MV=A-B (accc pcnblc ran ge (mg CaC03/L) = 75 to 125%) -Z-0 35 QS.07, s l amp. e measurements: Sample volume Be g in End Totnl Hardne ss S:imple number Sample ID (ml) ml m l ml Mul ti pli er (mg CaC01/L) T V=Nn Blank (ShOUIU uc u 111 1!, \..llL.'-'.Yt..J 0 t1:uJ1r i; 5D 1 7,0 11*!1 o.ti l,b.o 1e I 0 I=" 11-.'l ll1.3 Z.8 1 Ill . ., 1,6 3<.o 2J. / 1-{ a. t I I. It) 32 -r a'u J. 1..f 28 -tJ. b. y "1B I K ?i:J-S 3JS Bb L ol:S 1.1 3e

• M I .

JJ.tt O,') JO Not e: If >15ml o ft itrnnt is used, s am ple must be d iluted. Reviewed by: Page61of94 Date reviewed I oS*O\*\\ SOP C7 -Exhibit C7. I, re vision 01-01-I 3 l l l l l 1 l l J .J J J J J Analyst I vl_; Date analyzed t---O"-l.{---.-.1-5-.-j }----1 Hardness (SM 2340 C-1997) RL = l .0 mg CaC0 3/L Page _.3 __ Page 3 of_...;:3'--- Time initiated , .........._ Nl-e!lf-Time completed r:1 I Ir r 11 n . r I 1 ran norm a t 'V ant mu t/J. 1er t e ermma 1011: Titrant Normality check Begin End Total Normnlity (N) ofEDTA pH Fnctor or Multiplier re1,... stnndnrd ml ml ml 0.2/E = (N x 50000)/ SO ml samp l e number number (F.\ (ncccpt:t blc range 0.0180. 0.0220) -Nx 1 0 0 0 Laboratory co111rol stmulnrtf: Reference stnndard True vnlue Sample Hardness (MY) % RS = MY I TV x I 00 number (TV) volume Begin End Totn l Multiplier (mgCnC O ,>/L) (ncccptnblc range (mg CnCOJ/L) (ml) ml ml ml =90to110%) I JJSSM6} 40 50 a3.1 2..0 ZQO 40 I DO , l/J

• D r up, 1cate sample nrec1s1011:

Si1mple Hardness %RPO= Snmp le Snmple JD volume Begin End Totnl Multiplier (mg CnCOJfL) {CS* D) /f(S+D)/2(} x 100 number (ml) ml ml ml d) lJ ll/lJ.._,.. 1\J 26.'\ s .3 (o .* ,. 5D :j7'.':l 1.6 2.0 ... 0 : .. .. Duplicate (Bl) j 11.r 3q, S" i.e j D 3<.:? -o42eH atnx sot <e recovery: Reference standnrd Spike value Sample Spike hardness (A) number (SV) volume Be gin End Total Multiplier (mg CnCOJfL) (meC:tCO.>!Ll (ml) ml ml ml ( 4u 6U 3r."} c.tf .<<; w.o 70 Sample hnrdness (B) Measured spilce value (MY) % R = MY I SV x 100 (mg CnCO,>/L) MV=A-B (:1cccptnblc rnngc (mg CnCOJIL) =75to125%) '3cp 40 100.d>. S I amp. e 111ens11re111e11ts: Sample volume Begin End Total Hnrdncss Snmole number Sample ID (ml) ml ml ml Mu ltiplier (mg C:iCOJIL) TV "Rl::ink l'IU (shoul d be OmgCnCO.;/Ll

• {\I.{ 2:t31) OvW\h.._ 0 6D ao.'$"

Q.C\ a,o,,o /8 0 p I 12--() aQ.o b,!J..9-fl. a!M* O.f. 14 s o.:, ltJ.O -(" J..o.J. J..4.o o.<1 Iv v :20 \)

b. I L*D w 3,5.7 o:i.. ti.a Not e: If >15ml oftitrant is sample must I 'ilM.'T"I I. I ) . /7 Reviewed by: f' A I Date re view ed J .n Page 62of94 .... SOP C7-Exhibit C7.I , revision 01-01-1 3 l l l l l l l _.I __] J J _J J J 0 #!a.jj-C::J 0 .t 0 :. *;:J . . ..

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• E n vi r on men I al T utln!J So luU on s. Inc. Pag e 4 ---Page \ of LJ. ---Hardness (SM 2340 C-1997) RL = 1.0 mg Ca CO;IL Analyst I II-' ) Date analyzed ( r I I tr C 1 rmt 11orma tfJ 1 am mu 1p rer t eter111111at1ou:

Titr:int Normality check Begin End Total reference standard ml ml ml number number (E) 0.-0 \0.0 10.0 l b I I I (1 ornto r y co11tro slam an: Refer e nce s tandar d True value Sample number (TV) volume Begin End {mg CnCO.i/L} (ml) ml ml r 'N$ l'-\<t-:r 40 50 10.0 I\. 'i D /" I up 1cate snmp. e prec1s1ou: Sample S a mple Sample ID volume Begin End number (ml} ml ml 05.cH.(} 6Sw c;-o 4 Ouplicalc (B) _L atrix s11i <e recovery: Reference standnrd Spike value Snmple numbe r (SV) volum e Begin End (mg CaCOJ/L) (ml) ml ml -rLI£.<. +->-ID So r;r.w Time initiated T ime completed ..... ..._l-'4-'-'-l. -" 3:-'-----1 Normality (N) of EDTA pH Fnctor or Multiplier =0.2/E = (N x 50000)/ 50 ml samp l e {ncccplablc range= 0.0180 -0.0220) =Nx 1000 n O? ()0 Zo.<> Hardness (MV) %RS=MV/TVx 100 Total Multiplier (mg CnCO.VL} (ncccpt:tblc r a nge ml =90toll0%) I. q; 2-0.o 3\C'.C Hardness %RPO= Totnl Multiplier (mg C:tCOJIL} {(S-D} /l(S+D)/21} x 100 ml s .. 1-"1 'Zo.o 3i .*-: .. . . D ---£'!1<..- ).'\ .L os;-* .,..-4 Spike hnrdness (A) Totnl Multiplier {mg C:tCO.i/LJ ml .a.q -io.o -a Sample hardness (B) Measured spil<e value (MV) %R=MV/SVx 100 (mg CnCOJIL) MV=A-B {ntcc p tn blc rn n gc (mg CnCOJIL) = 75to125%) 38 es. <>5-0 ..... y 1.f-'40 )OO.o 0 Jo s l amp, e measu rem en ts: Sample volume Be gin End Total Hardness Snmnle number SamulerD (ml} ml ml ml Multin l ier (mgCnCO.i/L) T V=ND Blank (sho uld b e"' 0 mg CaCO;/L) 5o o.o D.o o.o -zo.o ....w (6.a;i.11-;YI ltSW .2&.\..\A 1-j. I '2&+."' '-\-. I ()6.0S,1). .J z.g ,'} '-\..tl. B'O l\ '1. --u..-,*H\ (n H-v..) \}., v ' 3'}.'5 i-\. !-\-egg 0'-\*'1.b-\\ & I 2 31-.5" .t.+,g 't;\p j 3 A. ...... \\Qg)\.0 \ .:je,l.l R .l5ot'"\ ) D.O 2.'l 2. _q si \ .:--... \ 2.. ?q &:\ I. SY. os. *S J 3 --_l--Fl.V.* Date review ed j \*1'1 No t e: If >15ml ofti trant is use d , sample must be dilu ted. Reviewed by: Page 63of94 SOP C7 -Exl1ibit C7.I , revision 01-0 J. IJ l l l l l l l J J J J t= m?;i'E . c *':-. -;( , ... ,, :. i : .i "*,i ! * -I -:---_ .. I . I * ... -'-... _., Pag e S Page 2... of 4 .._) En vironm c nta1 Totln_g SofuUons: , Inc. Analyst I Hardness (SM 2340 C-1997) RL = 1.0 mg CaCO,IL Date analyzed O'O*Ov "\ Time initia t ed I "-- --i Time completed . _ Ti' I ,. r ttrnnt 11orma ity anti 11111 /tfJ, ter llelermma/1011: ...... J a ....... uu. ... litv check Begin End Tot r ll Normality (N) of EDTA pH Fnct or or Multip Ji cr reference stnndnrd ,, ml ml = 0.2/E = (N x 50000)/ SO ml sample number number ---f--41?.l (acceptable range= 0.0 l80 -0.0220) =N x 1000 own--Lb I I I I I a oraforp con ro s arl: Reference st:indnrd True value Snmp t c Hnrdness (MV) % RS= MV /TV x 100 number (TV) volume Begin End Totnl Multiplier (mg CnC O.i!L) (ncc cp t nblc range (mg CnCOJ>'L) (ml) ml ml ml =90 to 110%) \ '--\-'&1-40 50 l:O.\.\-Jv.4 2..o 2J;>.o >+o I cc.o 0 b D r I up. tcate smm1. e pr ec 1s1 011: Snmple Hardness %RPD= Snmple S:implc ID volume Begin End Total Multiplier (mg CaCOJ/L) ((S-D) /((S+D)t21} x 100 number (ml) ml ml ml s -* .. *. .. \'"lb SQ\ *6 'l-5o 10.4 114-.q 1-\-.5 1..o.o 'lo '. . I .. l-Duplicate (B) j_ .1-D --as, 05' .* ..,,,_ IL\-."I l'lH-'lo afrrx sp 1 re recovery: Reference stnndnrd Spike vn luc Sample Spike h n rdnc s s {A) number (SV) volume Begin End Total Multiplier (mg CnCO,tL) (mg CnCOJ/L) (ml) ml ml ml I N5 s;, '-ten '-to So li+."1 2..1.'+ "'.'5 'Z,o . ..-:i \60 Snmple hnrdncss (B) Measured sp ike value (MV) % R = MY I SY x JOO (mg CnCO.i/L) MY=A-B (nc ccpl:tblc rnngc (mg CnC03/L) =75to125%) 'lo J-fC 100.0°/o S I amp e mens1treme11ts: Sample volume Begin End Totnl Hnrdn css Sample number Sample ID (ml) ml m l ml Multiplier (mg CnCO.i/Ll TV=Mn Blank -61Xt1rt-(SllOlllO OC V Oil! 1...aLU,;ILJ t.. ".>" ... .,""*or. 2..l '4-25.4. 1-1-.o -z_o.o '60 l.b j 3 ....L ,2o,Q >-h1a e>--4 llr.n ,, \ C::..""1 3o.o ,,., .:2. '} :.:t Ut_ . 'i ().S.C.'\. () \ ' 2 I '2.?.1 '1.C I 2.1! 11oso1o.o\ J 3 J_

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• i IJ}-P"1 2.5 \0,\, 14.\ L'Z-) \tlO Note: If >ISml o ft itr:mt is used , sample must be dilutecl. Reviewed by: rt Page 64 of 94 ..__ ___ ()' ....... Date re viewed I O .S* ()'-.. (\ SOPC7-Exhib itC7.l,rev ision01-0l-13 l l l l l l l J l J J J J Page Page 6 o f Y.. Hardness (SM 2340 C-1997) RL = 1.0 mg CaC0 1/L Analyst I Sc:it..-,;v Date analyzed OCQ. ou. )'"} TiU:o.11t uormalitv aml 11111/tip/ier tletermi11ntio11:

Titrnnt Normality enc ... " "' 'h End Total -0 reference standard ml ml llll number number (E} L b I I 1 a orntori1 co11tro stnm arl: Reference standard True value Sample numbe r (TV} volume Ile.gin End (mg CnCO J/L) {ml) ml ml 40 50 1&.\-.\ \\#.0 D r l up. rcate snm p, e prec1s1011: Sample Sample Sample ID volume Ile gin End number -r.JA (ml) ml ml \""\t.>SU'\-1.K ALF O'.J1 llf,O 1.\.1 Duplicate (B) J_ .l) .-:r 'A-:-S'" M .,, ntnx sp1 <e recoverv: R efere nce standard Spike value Sample number (SY) volume Begin End (mg CnC03/L) (ml) ml ml :f \.JSS I..\. '?o 2.S 2qs -. Time initiated,............._ Time completed 'S Normn lit y (N) ofEDTA pH Fnctor or Multiplier 0.2/E (Nx 50000)/ 50 ml snmple (acccptnblc range= u.u.;;_ n n??O\ Nx JOOO -........ f ... Hardness (MV) % RS= MV I TV x 100 Total Multiplier (mg C:iCOJIL) (ncccptnblc range ml =90to110%) 1.q 1,,o.o 3'B Cf'5.c 0 (, Hardness %RPO= Total Multiplier (mg CnCOJIL) {I S-D) /J(S+D)/21} x 100 ml 1-'2. ci .. 5.1-,,.,'?A>.o

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Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mgCnCO.i/L) T" .._TT"\ Blank

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---*-* rnc.uiteo 5 3'5.1-a,.'6 I.\ (In"\ 2.-Z..o 11'f>C(o4.oS (' N...rn -hi') 1?1 o 50 31n.1. IHO.C\ ""'* \ ez, -' A,.... 0 .. f vTU =-->-. l I U \ I _ _,, ...... a l,'T l 'I I . ,:'\ ' -I). ..... l I ............... -r ... "I lo\D ., . ' lD'-+ " '\ "-I I 0 .. \-I .. I '"T0.'1 "'?.'l.V I* " I. . \ 1 c.c:.o \ .o-4 ... \101 11.li \ f)o ,..,,..., 3.'2.-ll,.7-la'+ \1 o :.. '!>lo j 2 _L ;.:z. (i.? --I.Iv Note: If >151111 oftitranl is used , sample mu s t be diluted. Reviewed b y: CT\-\"'\ d{ Date reviewed I o 1 SOP C7 -Exhibit C7. I, revision 0 1-0 l -1 J l l 1 l l l l .1 J J J I J J J :" l '<.:\'j ' L_ I ;--*, --...___./ Pag e -=1-Page 1+/- of ) Hardness (SM 2340 C-1997) RL = 1.0 mg CaC0 3/L Analyst I SSv lfJ Date analyzed l'3' Time initiated O.sot#C1-Time completed "'4F I Titrn11t 11or111n/ity mul 11111/tiplier l etermimrtiou: --++<<---* "'-,rmnlitv check Begin End Total Normality (N) ofEDTA pH Fnctor or Multiplier reference standard -** ml ml = 0.2/E ... (Nx 50000)/ 50 ml sample number number r---fr;'\ (acccplnblc r:mgc = 0.0180 -0.0220) =Nx 1000 u lb I l I n orntorv contra stnm arr.: Reference standard True value Sample Hardness (MY) % RS= MY /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (ncccptnblc rnngc (mg CnCOJ/L) (ml) ml ml ml =90 to 110%) T\JSS 40 50 lq.5 I.Cf i,o.o C\S.o 0 fi, D I 11p, icnte sample prec1s1011: Sample Hardness %RPO= Sample Sample ID volume Begin End Total Multiplier (mg CaCOJ/L) ((S-0) /((S+D)/2)} x 100 number llvA-(m l) ml ml ml L'"\ \ 'i:° 101 4f'l4 3 5v 11.i 3!-+ t..o.o s '* .. ....... Duplicate (B) J_ l D l!6i.. JS.'2-3A J\!l ntnx sm (e recover,1: Reference st:mdnrcJ Spike v:ilue Snmple Spike hnrclncss (A) number (SY) volume .Begin End Tolnl Multiplier (mg C:iC03/L) IJ<.c. (mg CaCO:VL) (ml) ml ml ml 11.te-:} 1-\-0 5o rt.1 5.3 "],.o .o \O l.# -\lO S:imple hardness (B) Measured spike value (MY) % R = MV I SV x 100 (mg C:iCOJ I L) MV=A-B (acceptable r:rngc CaCOJ/L) =75 to 125%) t,i 38 <i.5.o°to ltt:&{ s l amp , e 111eas11reme11ts

Sample volume Begin End Total H:irdncss Sample number Snmple JD (ml) ml ml ml Multiplier (mg CnCOJ/L) TV=N O Blank

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' ?--Z0.3 ?..3.1.,, &,:; L,1..,, _J 3 '12 \,. 'h ... <t -'< I .Lo noso \. c'-1 -\JltSQ.v... \\)\ I 1 1.1#.'l \ ?>.'2.. L..14-\ "'\ I 2-dO.I 22. q 3$ L.\4 l\OSOS* ..l 3 ..... ....-1:2.il,, q 3ff .... -::Jo I I I -C$Q/*<-J-Date reviewed I O.S*OCo"l'1 Note: If >151111 o f titront is used , sample must b e dilulcd. Reviewed by: Page 66of94 SOP C7 -Exhibit C7. I , revisi o n 01*01-13 l l l 1 l l l l l J J J J ... %d Bf;f&l tf?/01 ,:. )*... ;-* -. -. '--.... :. I ' 1 *J. -* L::...J C..-."./ '\-------..... : Env il o n nnnt.al Tc.it Ing So l ullonJ. Inc. Analyst I \.U Date analyzed l)?.01-*\1-Ha r d n es s (SM 2340 C-1997) RL = I .0 mg CaC0 3/L g Page __ _ J Time initiated I iz:J 02 Time completed Titrnut u orma lit am l 111 11/ti l ier deter111i 11 11 t io11: Titrnnt Normality check Begin End r e ference standard ml ml number number Lnborntor co11t r ol st m u l11 rtl: Reference stnndnrd Tr u e value Sample number (TV) volume (mg CnC03/L) (ml) Total ml (E) Begin m l End ml Normality (N) ofEDTA =0.2/E (ncccptnblc r:mgc = 0.0180 -0.0220) o.ow o pH Factor or Multiplier = (N x 50000)/ 50 ml sample =Nx 1000 Hnrdness (MY) % RS= MY /TV x 100 Total Multiplier (mg CnCOJIL) (ncccptnblc rnngc ml =90 to 110%) 4 0 50 2\.<; 2..0 zo o 40 HX HI. D /" I u p /C(lfe samp e precm o 11: Snmple Hnrdncss %RPO"" Sample ID vo l ume Begin End Total Multiplier (mg CnCOJIL) {(S -D) /l(S+0)/21) x 100 number ,..,.___ (ml) ml ml ml s .. " Duplicate (B) D Matrix spike recovery: Reference stnndard Spike value Snmple Spike hnrdness (A) number (SY) v o lu m e: Begin Totnl Multiplier (mg CnCO.i/L) (mg C:iCOJIL) (m l) m l ml "'-.. Snmple hnrdness (B) Measured spike % R = MY I SY x I 00 (m1t CnCQ3/L) MY=A-B (n cccplnblc rnngc (mg = 75 10 125%) .. s I amp. e meosuremeuts: Snmple volume Begin End Tot:il H:irdness Snmple number Snmple ID (ml) ml m l ml Multio l ier (mg CnCOJ/L) TV=ND Blank {should be= 0 mg CaCO;/Ll 8J C,.<; C\.s o.o w: o tvo lO\ f 50 ,., ,-; :J4.v lo "2. \ z.. \ i Y*IP ,n*.£1 \\ 3 l 3 1.2. 3.'3 (Q (p -------/ M'f' .-----,_.---Date reviewed I 6S*Gi'\1 Note: If >151111 ofti1rn111 is used , sample musi be dilute d. Reviewed by: Pa!ffl o cl , l '1'-SOP C7-Exhibit C7.l , revision 01-01-13 Page 68of94 Sequoyah Nuclear Plant Biomonitoring Ma y 02 -09 , 2017 Appendix 0 Reference Toxicant Test and Control Chart -u :::r::: Pimephales promelas Enviro nm en t a l T es.unq s o 1uu on1 , I nc. Chronic Reference Toxicant Control Chart Source: In-house Culture 1.2 1.0 0.8 0.6 Control Limits(+/- 2 Standard Deviations)

---

.._ ...._. __ .__. __ _ ________ ,,,,,,_._

.. ----------. * * * * * * * * * * * * * * * * * * .,,,,,.,.,,----* ,_..--------------0.4 b.O -in d 1.2 > ta "'C I " 1.0 Warning Limits ................... ** .. * ............................... ......................... . **.--. .. -**-**-**-**-**-...**-**-**-**--**-**-** .... .__ ..

• 0.8 * * * * * * * * * * * * * * * *
• 0.6 -* * -* * -* * -* * -* * -* * .*-**--*** ---**-**-**-**-**-**---**-**-** __. .. .. , " , .............. ' .......................... .. ................................................................ , ...... 0.4 Test date e 7-day 1Cz 5 = 25% inhibition concentration. An est imation of t h e potass i um chloride c onc entration which would cause a 25% reduction i n Pimephales growth (calculated using ToxCalc). Central Tendency (mean loga rit hmi c IC 25 c onverted to anti-logarithm ic va l ue s) ---Control Limits (mean l ogarithmic IC 25 +/- 2 stan d a rd devia ti on s converted to anti-logarithmic values) -*. -* Laboratory Warning Limi ts (mean lo g arithm ic IC 25 +/- 2 coefficent of varia tion s converted to anti-logarithm ic va lues) * .... -. . . USEPA Warning Limits (mean logar i thmi c IC 25 +/- SA_75 con ve rted to anti-logarithmic values, sA.7 5 = 75 1 h percentile o f CVs reported na tio nally by USE PA) Page 69of94 lQ CD Soknlotn.

ll'K.. ........ c Pimephales promelas Ch r onic Reference Toxicant Control Chart Source: In-house Culture Lo g 10 Conve rsi on Anti-log a rithmic Valu es (g/L KC I) co T4',t n umbe r Te st d a t e 7-day IC 25 Labo r atory C a lculated CV ToxCa l D eterm i nation 7-day IC 25 CT s CT Con t rol Lim it s Warn i n g Lim i ts (g/L KCI) CT-ZS CT+ZS CT-ZCV CT +ZCV 1 04-04-16 0.7240 -0.1402 -0.1647 0.0544 0.6844 0.5328 0.8791 0.4629 0.9689 2 04-05-16 0.7762 -0.1100 -0.1569 0.0538 0.6968 0.5440 0.8925 0.4775 0.9777 3 05-10-16 0.7510 -0.1 244 -0.1528 0.0511 0.7034 0.5559 0.8899 0.4937 0.9685 4 05-17-16 0.6409 -0.1 932 *0.15 73 0.0496 0.6961 0.5539 0.8749 0.4918 0.9530 5 06-07-16 0.6998 -0.1 550 -0.1571 0.0468 0.6965 0.5614 0.8640 0.5026 0.9370 6 06-1 4-16 0.7412 0.13 01 -0.1546 0.0451 0.7004 0.5690 0.8G23 0.5127 0.9315 7 07-12-16 0.7965 *0.0988 -0.1 5*J O 0.0460 0.7080 0.5729 0.8 7 49 0.5172 0.9437 8 07-19-16 0.6348 -0.197 4 -0.1536 0.0459 0.7021 0.5682 0.8674 0.5114 0.9376 9 08-02-16 0.7850 -0.1051 -0.1502 0.0460 0.7077 0.5726 0.8746 0.5168 0.9436 10 08-09-16 0.6703 -0.1737 -0.1517 0.0447 0.7051 0.5739 0.8664 0.5190 0.9339 11 09-12-16 0.7241 -0.1402 -0.1510 0.0433 0.7063 0.5786 0.8622 0.5255 0.9270 12 09-12-16 0.7351 -0.13 36 -0.1 500 0.0 4 21 0.7 0 80 0.5831 0.8596 0.5315 0.9222 13 10-04-16 0.7791 -0.1 084 -0.1477 0.0420 0.7117 0.5864 0.8638 0.5357 0.9254 14 11-01-16 0.7808 -0.1074 -0.1456 0.04 1 9 0.7152 0.5897 0.8674 0.5398 0.9280 15 12-06-16 0.8303 *0.0808 -0.1423 0.0433 0.7206 0.5904 0.8795 0.5399 0.9411 16 01-10-17 0.7066 -0.1508 -0.1433 0.0432 0.7189 0.5892 0.8773 0.5384 0.9392 17 02-07-17 0.7 052 -0.1517 -0.142.1 0.0426 0.72 0 9 0.5924 0.8772 0.5426 0.9378 18 0 3-07-17 0.7620 -0.1 1 8 1 -0.1362 0.0365 0.7308 0.6178 0.8646 0.5 7 61 0.9139 19 04-04-17 0.7043 -0.1523 -0.1392 0.0351 0.7258 0.6174 0.8531 0.5765 0.9012 20 OS-02-17 0.6740 -0.1714 -0.1371 0.0316 0.72 9 2 0.6305 0.8434 0.5939 0.8858 Note: 7-day I C is = 25% i nhibition c o ncen t rallo n. An e st i m ati on of the potassium chlo r ide concentrat i on th a t would ca u s e a 25% reduct io n in P l mepholes growth (calcula ted u sing T oxCalc). CT= Central tendency of the ICn values. S = Standard of the IC 1 s va l ues. Co ntro l Limi t s= Mean logarithmic IC 15 +/- 2 st and ard dev l at l ons convert ed to a nt -logarit hm ic values. Warni n g Limits= Mea n logarll hm lc I Cu +/- 2CV ors. 75 con v e rteCI to ant i*loga rlthm i c values , S,.n = Standard deviatio n correspoM i n g to the l':i' percentile ol lVs reported n ati on a ll y bv USEPA is.,.= 0.38). CV= Coe(flclenl of variation. 75th Percentile CV W a rn i n g Lim i ts CT-S A.75 CT+ S A.7S 0.4243 0.9506 0.4320 0.9679 0.4361 0.9770 0.4316 0.9669 0.4318 0.9674 0.4343 0.9729 0.4389 0.9834 0.4353 0.9752 0.4388 0.9830 0.4372 0.9794 0.4379 0.98 1 0 0.4389 0.9834 0.4413 0.9886 0.4434 0.9934 0.4468 1.0009 0.4457 0.9921 0.4469 0.9948 0.4531 1.0086 0.4500 1.0015 0.4521 1.0063 .s= ...... 0 .... \.!) 0 ... ...... c: 0 u 0 0 c: .52 ... "' ro > .._ 0 .... t: Cl.I u :E Cl.I 0 u Cl.I ro :> .... ro ro :-e c: ......... tlO E -..c -3 0 .... l!) 0 .... ... c 0 u .... 0 .... 1.25 1.00 0.7 5 a.so 0.2 5 20 10 0 30 2 0 1 0 0 Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture ------------------------* * * -----..... .-., . ------------------------------USEPA Mi n imum Acceptance Criteria (> 0.25 mg per surviving larvae) USEPA Maxi um Coeffi c ient of Variation G u i d ance C r iter i a(< 20%) -----------------* * * * * * * -..-.. ..---._ ------------------------USEPA Upper PMSD Bound Acceptance Criteria(< 3 0%) USE PA Lower PMSD Bo u nd * ---..------___ __.....-* * * * * * * * * *

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">,,:...b r;,bsb cv">b c b'">b 1Y'°1 01:...1 01:...1 &..,.1 0 ,_..">1 0 o.!u 0 o.*0 r;:,s-"> r;:, b"' cb*'\J 0 1-">-r;:,1 *,. r;:,'b' r;:,'b* r;:,"J' c"J* '\.c-..,_v ">1.: r;:,V "> r;:,1.* r;:,, , c°" 0 c,* Test date

• Contro l Growth , Coeffi cie n t o f Variat i on {CV) or Percent Min i mum Significant Difference (PMSD) PMSD is the percent mi n imum significant difference between the control and treatment that can be declared statistically significant.

The lower PMSD bound represents a practical limit to the sensitivity of the test me t hod and is nor a minimum acceptance criteria. Central Tendency (mean Control G r owth. CV or PMSD) 95% Confidence I nterval (mean Co nt rol Growt h , CV or PMSD +/- 2 Stan d a r d Deviatio n s) Control Pimepha/es promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ToxCa l Determ i nation Control Growth Control Growth CV Control Growth Test (mg/uu 1 l1l larvHI !l<I T est numbe r Test date S urv ival Mean C V MSD PMSD CT 95% Confiden ce Interval CT 95% Confidence Interval (%) (mll/ln l lla l l*rvae) (%) (%) CT*2S CT+ 25 CT *25 CT+2S 1 04-04-16 100 0.461 5.9 0.0395 8.6 0.6 125 0.4 15 0.810 5.3 0.0 10.6 ?. 04-05*16 100 0.647 1 2.3 0.1138 17.6 0.617 0.4 35 0.799 6.3 *0.8 13.S 3 05-10-16 100 0.54 1 7.0 0.0614 11.3 0.608 0.431 0.7 85 6.4 *0.2 13.1 4 05-17-16 100 0.507 1.8 0.0438 8.6 0.597 0.418 0.775 5.9 -1.0 12.9 5 06-07-16 100 0.421 5.0 0.0592 14.1 0.5 7 9 0.377 0.781 5.8 -0.7 12.4 6 06-14-16 100 0.455 3.2 0.0334 7.3 0.568 0.362 0.773 5.6 -0.B 12.0 7 07-12-16 100 0.578 8.8 0.0763 13.2 0.56 9 0.373 0.7 65 5.9 -0.5 12.3 8 07-19-16 100 0.774 10.1 0.1053 13.6 0.5 8 4 0.365 0.804 6.2 -0.4 12.7 9 08-02-16 100 0.570 3.4 0.0474 8.3 0.583 0372 0.794 6.0 -0.S 12.S 1 0 08-09-16 100 0.699 3.5 0.0827 11.8 0.591 0.379 0.803 5.8 -0.6 12.2 11 09-12-16 100 0.604 S.1 0.0532 8.8 0.592 0387 0.797 5.8 -0.4 ll.9 12 09-12-16 100 0.637 4.8 0.0520 8.2 0.5 9 5 0395 0.7 94 5.7 -0.3 11.7 13 10-04-16 100 0.588 3.2 0.0506 8.6 0.594 0.4 0 1 0.78 8 5.6 *0.4 11.5 14 11-01-16 100 0.671 7.7 0.0627 9.3 0.5 98 0.A0 7 0.7 90 5.7 -0.2 11.S lS 12-06-16 100 0.617 S.6 0.11 67 18.9 0.599 0.41 3 0.786 5.7 o.o 11.4 16 01-10*17 100 0.587 7.3 0.0629 10.7 0.600 0.414 0.786 5.7 0.0 11.3 17 02*07-17 100 0.579 11.5 0.0751 13.0 0.5 91 0.420 0.763 6.2 0.3 12.0 18 03-07-17 100 0.638 6.2 0.0662 10.4 0.590 0.421 0.759 6.2 0.4 12.0 19 04-04-17 100 0.620 5.5 0.0919 14.8 0.591 0.<1 2 2 0.761 6.3 0.6 11.q 20 05-02-17 100 0.729 5.6 0.0792 10.9 0.59 6 0.4 1 6 0.776 6.2 0.6 11.8 Nore; C on tro l S u rvlv af = USEPA min mum te.n u'eo t abUtty c11teria l. 80".4 s1.1rv1va l. "'o ntro l Me a n G rowt h:;:;: USEPA mloimutfl rest cntena 0.25 m1/surv1v ng l ilNU*. or \'irtiJ:dor fo r contro 1 1rowth. USEPA miJ:dmum (\/ (90 1"' percenlite, < 10% M S D

• M i n i mum >i&n l ftcont d fleronce. PMSO Pcrctnt m i n imum Stgn 1 f 1 nttt difference. PMSO , ,. .a measUt of prec i sion. The PMSO t s 1'1e m 1 n11nurn percen t difte , tmce betw,en t he control and l rHrmec , t th at can be: declarf d ngn i flcitrn In 1 whote elftucm t to'l!I C l ty t Mt. Lower PMSD bound determ i ned by USEPA (10'" percon 1 1 le) > 1 2%. 'The PMSO bound rep r esents .i pr.c t icaf ttmi r to the senm lVl tv of tM te s t rM t tiod ,;,nd I J not 1 m i n.m u m 1<<epu1nce a 1 tena. Upper PMSD boond *a:eptonco criter i a dotermln e d bv USEPA (90" percen1llel

< 30%. ct'=: CP n t r."I r ia ntf*f\(\I ot th! l'OWlh , CV o r S t a ndard devi1tl0rlot t he growth , C\/ o' PMSOv lues. CT 10.7 11.7 11.7 11.3 11.6 11.2 11.4 11.5 11.3 11.4 11.2 11.0 10.9 1 0.8 11.2 11.2 11.4 11.2 ,, ' 1 1.4 Test PM50 (%) 95% Confidence Interval CT* 25 5.8 4.8 5.3 5.0 5.4 4.8 5.2 5.5 5.2 5.5 5.4 5.2 5.1 5.2 4.6 4.6 4.8 4.7 4 7 5.0 CT+25 15.7 18.6 18.0 17.6 17.8 17.6 17.6 17.6 17.4 17.2 17.0 16.8 16.6 16.4 17.8 17.7 17.9 17.7 17 9 17.8 .., ;:-""' 0 r-:-::-:i r--:-i ,,,.-...-.

• I 'I-'-' --* I *--1 ** I --. ... tnYironrMnulTf'st l ng Sc.lo1'°"s.

l1K. Page I of6 Potassium Chloride Chronic Refer e nc e Toxicant Test (EPA-822-R-02-013 Method 1000.0) Species: Pimeplzales prome/a s Dilution re arntion info r111atio 11: KCI Stock lNSS number: lNSS \ S&i.o Stock preparation

Di l ution prep (mg/L) 300 450 Stock volume (mL) 9 13.5 Diluent volume (mL) 1491 1486.5 To t nl volume (mL) 1500 1500 Test oruan;sm informati o11: Onranism source: Age: Spawn date: Hatch dates and times: Transfer vessel i nfo rmatio n: Average transfer volume: 50 g KCl/L: Dissolve 50 o KCI in 1-L Mi ll i-water. 600 750 900 18 22.5 27 1482 1 477.5 1473 1:500 1500 1500 In-house culture < 24-ho ur s old <:>'>*Ci\*\"'\

\""\OQ o<:..-o-i.-n 1050 31.5 1468.5 1500 pH= \.21.. S.U. Temperature = °C < 0.25 mL Daily feeding aud renewa l i nf ormation: D:i y D:itc Morning feedin g Afternoon f eedi n g Time Ana l yst Time Analyst 0 05-02-17 ()o\0 X{ u I ... " 05-03-17 ,.{ !< 2 05-04-17 D\oO C,, n ... \,I. \1 3 u 05-05-17 Ob\C \"l.l b l( 4 05-06-17 " C"l\ c; 5 05-0 7-17 0"#\0 't\ \ "!l'\C H 6 05-0R-17 Olo \<':, }t \'1.\.<.> i\\ 7 v 09-17 PpKCICR Test N umb er: 25 Comments: Test iuformntion: Randomizino tem late: Incubator number and shelf lo cation: Arte mi a CHM number: CHM914 Drying information for weight determi11atio11: Date I T im e in oven: Initial oven tern erat ur e: lDa*c_ Date I Time out of oven: O'MO*"('\ 0 Final oven tern eratu re: Total drying time: Tes t in itiation , renewa l , l\fl-ISW or termin:ition batch usccJ Time An:ilyst "1..0 o'-l*u.*11 A ' "' 0'\* 11o. ,, o+t.b*f'\ & 0&'°'40 \\ o'-l * ', "' ol\\5 i\ O'\t..\O At OS*Ol.*'1 o'\01-Ai CS*<n.. ,, o i "to -Control illformalion: Acceptnnce cri t eria 0 . $20% Average we i ght per surviving larvae: 2: 0.25 mg/larvae Page 73 of94 SOP A T 21 -Exh ibit AT2 I. I , revision l 1*01-14 '"i i

I --I. * . ...__ Page 2 of6 ,. -, I :*-----. _ _.../ ) So I 11Uans.lr.c..

S p e cie s: P imeplz a l es pr o m e la s PpKCICR Test Number: 25 S urvi va l a n d G ro w th D a t a Day Con tro l 3 0 0 m g K C l/L 450 m g KC l/L A B c D E F G H I J K L 0 10 10 10 1 0 10 10 10 10 10 10 10 10 l /D <o I O to 10 I O 10 J o 10 10 IQ 1 a 2 lQ to (O {Q IO IQ \C) LC) lo IO 10 3 I\) (Q lb I() IC) 10 (() 10 I\) I {J IC 1 0 4 10 10 /0 JO 'O IQ /0 IO ID I() IQ IQ 5 I 'O It) ID IQ LD { () IQ ( () co IQ LO 1 0 6 J U m f{) (0 lO 10 I () 10 IQ l() lO 10 7 ft) I() I D 10 r o t o lb 1 0 ID (() 10 ., 0 A =P an weig ht (m g) Tray color code:: U:g h I-BL" .e. 15.IS j\,,}.S lb.'18 1 5.'°40 1-:iOO IS.e=f IS.3s llo.18 ls. °37) Annlyst: 'i--Date: 13 = ran+ Larvnc weight (mg) ?13.88 *. oe 22.cH io.'¥1 Ana l yst: lA...J citl.'5l> Date: os.11.n-C =Larvae wc.ight (mg)= B -A H a nd cnlculntcd. , , olo ". &?>> <..to S , t\"L '-* () 2 ,.'?..'\ Co.SC\ s. *C. c.. a1 SA"\ Ana l yst: Weight per inil i nl number of la r vae (mg) =C f In i tial n u mber of lnrvne fl.,,., oi "'6'.. 6' }-It') ..,0 (J..'V Hnnd cnlcu l:i t cd. £ " t.; \0 t ,... ,... '1) "' '? \!) "' . A1rnlysl: o* 'V . C)' 'V' 'I;) o* t$ (). (). '()" o* Average weight per inilial Pc recur ,_ ... ..--.. ..-number of J:ir"ae (mg) re d uction r. ef .* \ '2. .'\ 1. o.

n. & <. 0 .11..'\

-.. 0 f f rom con l rol * . *'> ("/o) .:-.: .. *-. Comment codes: c = clear , d = dead, fg = fungus , k =killed, m = missing, sk =s i ck, sm = unusually small , lg = un u sually large , d&r =decanted and returned , w = w o unded. Co mm e n ts: Page 74of94 SOPAT21 -Exhibit AT21.J , revision I J-01-14 P ag e 3 of6 EIWf ron men 1 aJ T ei l in9 SG l utJO ns., l n c. Species: Phnep!tales promelas P p K C ICR Test N umber: 25 Survival and Growth Data --Day 600 mg KCl/L 750 m17 KCl/L 900 mp KCl/L M N 0 p Q *R s T u v w x 0 10 !O 10 I O 10 JO J O 10 1 0 1 0 10 10 J 10 ID 10 ( () co IO "11{ 10 '\ t .( IQ I{) I l) 2 10 10 10 tO 10 r o ro '() 10 °" 3 10 ID ID l () ID t() J 0 I() 10 '\ °' 4 l#. "l-'. , .. '"' (0 LO 'D io l \.) " K & '\ !' .f 5 '6u.. 10 IQ \ '"' f '"-., ).\ "'° 6 g 10 " 10 \ -,'A l , lo ----7 \ () H) \ ., , ..( t \ "" \. \.. A= Pan weight (m g) '1.u 1c;,qz. Tr:t y color code:: :=.h:qM e. l'i6Lt IS.e;.t l"i.llv IS.l.I.{ ll0.12, I IS.03 IS.le Analyst: Date: a.";}. B .. Pan + Larvae wei g ht (m g) Do.41 l.t.ll. 2o.£fz. \'t1S IS.&=\ 2o.11 Analyst: l'f °' 20.2e Dale: o:;_ u.1-:r C = L:irvac wei g ht (m g)= B -/\ Hand calculated. S.ii 1...4'; i.. c'-\ ".iii. 'i.8'1 q.to t.(.U \.\ :>.1.-1 Analy s t: J 'Weight per initial numll c r of larv a e (m g) =CI Initial numb e r ofl:1rvnc ,.. N "'o t\/ $' v,O .;j Hand cnlculnted. 0 v .)..: ::;;. .).. '? Analyst: . " t)' o* o* o* 0 tr "' o* o* c;)' A\'erngc weight per initial Percent number of larv a e (mg) reductio 11 from c ontrol o.1o'2.& 1. 0.

o. 1. (%) Comment codes: c =cle a r , d =d e ad , fg = f ungu s , k =kill e d , m = mi ss in g , s k =si c k , s m = un us u a ll y sm a ll , lg= unu s uall y lar ge , d&r = de ca nt e d a nd ret u rne d , w =w o u nd e d. Comme 11t s: Page 75 of94 SOP A T 2 l -Ex hibit A T 2 l .1 , r e v is i on l 1-0 1-1 4 0 I I L-::J r;::J 0 : . ,-. '-* -. . S p e ci es: Pim e phal es pro me l as PpKCICR Test Number: 25 S ur v i v al mu/ G ro wt h Dain D ay 1050 m g KC l/L y z AA BB 0 1 0 JO JO 10 l t. "\...\ lo 2 l.. .... !Q'" (g to 3 '-\.,,.,. , .... \D \, s 4 ,;,. v.... w.. "'.I. "'L L-5 , .... '\A '" l t "'Z... \ 6 '" '" c"' \ () \ 7 \ t:> \ 0 A= P nn weig h t (m, .ci. ,..,...,.. 'i.. Tray co l or code::

l110'i 11o.l8 Ana l yst: Daic: B =Pan+ Lan*ae 11*eigb1 (mg) 11.ot 7 n:1ly t: """" D ntc: 05. }l .j'.}:: 6t C"' Larvae weigh I (mg)= n -A o."10 Hnn tl cn lc u l a t cd. 0 ;1'&) ... Analys t: I> .. ,. ..... Weig hl per in i tial nu m ber ofl:tr\'ne (m g) "'CI I n itia l numbe r o f lnr\'nc 0 \..' 0 Hnnd enlculatcd. <:> Analyst: ()' ()' Avcrnge weight per i n i li al l'crec n t number oflnrvnc (mg) rcducrion from con t rol (%) Co m me n t codes: c =clear , d =dead , fg =fungus, k =killed, m =missing , sk =sick, sm =u n usua ll y small , lg = u nu sually large d&r = decanted and returned, w ,,.. wounded. Co mm e 11 ts: Page4 o f6 Page 76 of94 SOP A TI 1 -Ex11ibit A T21. I , revision 1 1-0 1-14 ,_.,,.,.11o"'"'"l""' ConUol JOO 450 600 750 9U U 10$0 >unnctrs MSO 'lil l ue i 'NISD: "*.Mtel* A 8 c D E F G H I I K l M N 0 p 0 R s T u v w x y z AA 88 111111*!......m\*tDI rl".tm.mb.,ot ,...,_ .. t ttit(,.-icJ '*"'** ...... 10 10 IS.IS 10 10 lS.3S 10 10 !6.2S 10 10 ls.98 10 10 15.40 JO 10 13.42 10 10 15.00 10 10 15.1!9 10 10 15.35 10 10 1618 JO I 10 10 10 1530 10 8 14.54 10 10 15.92 10 9 1 51"' 10 10 14.96 10 7 l S.2* 10 7 16.12 1 0 7 1 4.9 7 10 g 15.()3 10 7 15.95 1 0 6 IM O 10 6 15.78 10 6 16.57 10 I 16.** 10 0 0.00 10 I 16 IS 10 0 0.00 0.0 7 92 M$D: 1 0.9 PMSO: Plm e pho/es promelo s C hronic Refer e n c e ToK i c a nt Te s t E PA-821-R-02-013 , Method 1000.0 Qu a l i ty Cont r ol V er i'i c at i on of D a ta Ent r y , C a lcu l a t ion s, nd S t at i st i c al An al yse s I *Patt*l*fn* a...,..,,,. ... ,.,, (rnd* Wa ltft t/S**vt,...* 1 M. ... Swvtwf*& C'a t md*"lofvwlaciori wefcht(md ... nwnbu of hrv** Imel 11umtt.1 ol tr"'n lm1l ..... _. .................... -**1ooo* .... 1tKI 22.7 8 7.63 0 7 6? 0.763 22.41 7.06 0.706 0.129 5.6 0.706 23 88 7.63 0 , 763 0.763 22.81 6.83 0.683 0 , 683 2150 6J.Q 0.6 1 0 19.34 S.9' 0 592 O.E35 10.0 0.592 21 08 6 08 o.608 0.608 23.l& 7.Z9 0.729 0.729 H.94 6.59 0.659 0.659 12.04 S.86 0586 O.!i99 80 0.5!6 2079 6.07 0.607 0.607 20.74 5.44 0.544 0.54* 2041 5.87 0.734 0.587 22.37 6.45 0.645 0*81 S.5 000 ll.BS 6.04 0.671 0.60-I 21.n 6.76 0.676 0 , 676 20.08 4.84 0.691 0.48.> 20.92 4.80 0.686 0659 6.1 19.19 4.22 0.603 0412 10.2 8 5.Z 5 0.656 O.S15 20.65 4 , 70 0.671 O.A70 1 9.7S ,,n 0.725 14.l 0.4J5 0.630 18 89 3.11 0.518 0.311 20.19 3.6? 0.603 0362 17.07 o.n 0.730 0.073 0.00 0.00 0.000 0.:10 7.l 0000 1699 0.81 0.810 0081 0.00 0.00 0000 0.000 MtlWl'tum Slc n Fic.lnt Olffe,..,,CC! Min i mum S*snlfte*nt Difference TOll n u mber: 2S T*tt dates: ________ M_*v_o_2_-0_9_._20_1_1 M .. w.fit\l/ Cocffhflf'll Of Wot tUOI\ r.u'" r*dl.kUon ""' ttu l'l\beroJ l11 rvH 11 (Gft!Ut f '"'* tml) 100.0 0.729 5.6 N ot applica b le 1 00.0 0.63 5 10.0 12.9 100.0 O.S99 1.0 17.8 9 2.5 0.628 6.4 13.8 71.S o.*18 8.9 34.4 6 2 .. S 0.395 1 8.1 4 S.9 s.o 0.039 US.8 94.7 PMSO ti a rnHuirt of tut i:iredsico Tht PM.SO b m i nimum otr(ent cllfferenct: t>etwnn the c°"trot 1nd trutrnenl that ati be decf.-rcd il1ttistieallv I n a wttole effluent tow 1 dty test Lower PMSO bound cleterMined brt USEPA ( 101h PfHC'tnt ll e) :s 1 l t-4 PMSO bound det*rmln cd by USEPA (90th pe r c:enllle).;;: 50'4. l ower and pp.er PMSO boufld\ wen de t crminud from the 1 0th 1 nd 90th pe r nntile , P.t t1 ety , of PMSO d:.lt;i fr om (PA's WET ll'tterbbOr.Horv Slud°' USEP". The f owe1 PMSJ bot m d ropunef'IU

• PrKllu l llm{l lo i ansi tw l h of the test method iltid is not a m i nimum critcrii tJS!PA 20011 , 2001D. Flna l Reoon: 1 tut1rl1tDOn f Ol"J vat 1abU11y !:t.hJov of U'o\ Sho r t-te.rm Chtonie at\d Acute Who le Elt l\Jent To*icltyTes t Me t hoch ,

1 tl'ld l*Appctnd l'(. EPA*ll:J..S..01..()04

and EPA.-321-1

• 0 1..00S. us E nv lfor\men ta l Prote<t i on Agencv. OH.

St.art oa e-51212011 E nd Date: 51912017 Sample Date: Comments: Conc-m IL D*ContrOI 300 450 eoo 750 '900 1 050 Conc-m9/L D-Contro l 300 450 600 750 "900 "1050 Auxllia!l'. Tests 1.0000 1.0000 1.0000 0 8000 0.7000 0,7000 0.1000 Mean 1.0000 1.0000 1.0000 0.9250 0.7250 06250 0.0500 2 1.0000 1 0000 1 0000 1.0000 07000 0.6000 0.0000 N-M 11a n 1 0000 1.0000 1.0000 0.9250 0.7250 0 6250 0.0500 Sta tistica l Analyses Larval F is h Growlh an d Survival Test-7 OaySu.rvlva l Test ID: PpKC l CR ID. REt>-RefTOAi1,;Cill\ Lab ID* ETS-Envir. Tes ting Sol. Samp l e T'fpe: KCL-Po t ass i um c:hlof!de Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimepha l es promelas J 4 1.0000 1 0000 1.0000 1.0000 1.0000 1.0000 0.9000 1 0000 0.7000 0.8000 0.6000 0.6000 0.1000 0.0000 Transform: Arcs l n S9uarc Root Rank 1-Talled Number Mean Min Max CV% N S 1 1m C rltlr.:ll R as p 1.4120 1.4120 1.4120 0.000 4 0 1.4120 1.4120 1.4120 0000 4 *a.oo 10 00 0 1 A120 1.4120 1.4120 0.000 4 " 8.oo 10 00 0 1 2951 1 1 071 1.4120 11 347 4 '4.00 10.00 3 1.0202 0.9912 1.1071 5.685 4 10 00 11 0 9123 0.8861 0.9912 5.759 4 1 0.00 10 00 15 0.2403 0.1588 0 , 3218 39.161 4 *o.oo 10.00 38 Statis ti c Critica l Skew Shapi r o-Will<'s Tas l i ndicates non-normal distribution (p <= 0.01) 0.87827 0 896 -0.3Be8 o* vanance cannot be connrrned Test (1-tan, 0.05! NOEC Steel's Many-One Rank Test 600 Treatments vs 0-Control Trim Level EC50 95"/. CL 0.0% 5.0% 10.0% 876 51 S34 62 920.49 20.0o/o 897.43 8483 7 949.33 Aul°'S.0% 865.76 826.27 907.13 09 08 .. 07 > 0.6 " "'0.5 ,.. Cl 0 4 r-03 0.2 0.1 0 g g c ... 0 ll 0 Page 78of94 LOEC 750 c.nv TU 670.82 Trlmme d Spearm a n-Ka rber 09 08 07 :l 0.6 c &. 0 5 .. Od 03 02 0 I Dose-Response P lot 10 100 1000 10000 Dose mglL Total N umbe r 4 0 40 40 40 40 40 40 Kurt 1 96258 Stan Da l e: 5/212017 End Oa1;i* 5/9/2017 Sample Date Comments: C o n c-m IL 0-Control 300 450 600 750 900 1050 0.7530 0.6100 0.6590 0.5870 0.4840 0.4700 0.0730 2 0.7060 0.5920 0.5860 0 6450 0-4800 0.4350 0.0000 Stat i stical An a ly ses La rv:J I Fish G r owth a n d Surv i val Tc s t-7 Day Growth ID* Ppl(CtCR S;omp l A tn* RFl'°-RAr Lab ETS-Env i r. Testing S ot Samp le Typ . KCL-Potasslum chloride Pro1Dco1 FWCHR-EPA-821-R-02-013 Test Spec1 s PP-Pimephales promelas 3 4 07630 0.6830 0.6090 0.7290 0.6070 0.54'50 0 6040 0.6760 04220 0.5250 0.3110 0.3620 0 0810 0 0000 T rans form: Unt ra nsformed 1-Tafled I s oto nic: Co nc*m!l'L Mea n N-M ean Mo a n t Jl i n Ma x CV% N t-Stat C ri ti cal M S D 0-Contro l 0.7288 1.0000 0.7288 0.6830 0 7630 5 578 4 *300 0.63 4 8 0.8710 0.6348 0.5920 0.7290 9.980 4 2.718 2.290 0 , 0792 *450 0.5990 0 8220 0 5990 0.5 440 0.6590 7.982 4 3.752 2.290 0.0792 *soc 0.6280 0.8617 0.6280 0.5870 0 6760 6.403 4 2 913 2.290 0.0792 750 0.4 778 0.655 0.4778 0.4220 0.5250 6.868 4 900 0.3945 0.5413 0.3945 0.3110 0.4700 18.1 4 3 4 1 050 0.0385 0.0528 0.0385 0.0000 0.0810 1 15.781 4 A uxillal"t'. Tests S tatistic Cr it ic a l Shapiro-W i lk's Teslindicates normal distribution (p > 0.01) 0.92374 0.844 Bartlett's Test indicate$ egual variances(!!= 0.66) 0.76291 11.3449 Tes t (1*Uil , 0.05) NO EC LOE C ChV T U MS D u MSD p M SB MSE Ounnett's Test <300 00 0.07919 0.10867 0.01266 0.00239 Treatme n ts v s D-Contro l Li n crar lntcrpolot l on (Z OO R c::;am plc:;) Poin t mg IL SD 1co5* 116 29 61.66 1c10* 232.58 85.50 IC15 408.09 138.74 IC20 633.70 58.72 IC25 673.96 19.94 IC40 822.97 41.45 IC SO 912.69 18.66 95% CL (E x p) S kew 54.14 436.34 1 8870 108.28 6D2.01 1.6404 126.89 754.60 0 3100 316.33 686.56 -3.8530 613.55 747.38 0.2 701 7 15 37 958.28 0 3965 829 43 945.96

• 1 0693 0.9 0.8 07
• indica t es IC es timate le$$ than the lowest conc.en t ration =: 0.6 0.9 0.8 07 i 0.6 e o.5 Cl :04 0 ... 0.3 01 0 I 0 Page 79 of94 e c 0 0 6 c: g_ 0.5 .. 04 OJ 0.2 01 Do se-Re s pon se Plot 500 1000 D o se mg/L 1 -!all , 0.05 leve l o f s gnlllcance Me a n N-llle an 0.7288 l.0000 0.63 4 8 0.8710 0.6135 0.8419 0.6135 08419 0 4778 06556 0.3945 0 5413 0.0385 0 0528 S k.e w Ku rt 0.70843 -0.4102 F-Prob d f 0 0148 3 12 1500 Page 5 of6 . ., .* 1 S p ecies: P i m e plzal es pro me la s PpKCICR Test Number: 2 5 D ail y C!t e mi s t1 y: D ay (Analvsl identified for cnch day, pcrfom1ed pH , D.O. and cond uc t ivity measurements onl v.) 0 I 2 A n a l yst 1l1 t-.,;... IL-"'"" Co nccn tmt ion Pa r amete r : .;-'. -* Q* .. -. -. -

,_ -*" * .. *--. _.__ l. . -- pH(S.U.) g,2.\ e>.10 "t.':\Z. e.oe D O (mg/L) 't.!1 '.J. .to +..:r /l .. Y Conducfi\'ity .. .. (1 1111hos/cm) 32..\ "* -32.6 .. * '3 t<i' I* ' . ; ; .. -_> -* C O NTR O L "A lkalinity . -r..,: *;;: -. -. ' CaCOJIL) , .. -* *: ,,,* .. --. " H:irdnc s -'.'[ , ,. --...... ;': . .. -.,. (mg CaC01/L) . ,,_. -*Te mperat ur e (0 C) ,_'\."'\ "\S. '1. ,.,1..\. \ 1."'\.l.o "'\.&.\. p H (S.U.) 0.'1* ".l.e>j 1-\40 So,p '\.-s:1 D O (mg/L) f).._\O "+.'l 1-..d ".\A JOO mg KC l/L C n n<l nrtivity l "'* , .. eq0 ett \ . (um hos/cm) . ( . ' . -*Te m pera t ure (0 C) "1.'\.i... 'L..S."\ -u>.,.\ "l.'-\*S \.."\*'\ pH (S.U.} e.\'5 &O<P 0()5 '.} *"' l DO (mg/L) '}.'\ 'l.ta '+.3 /l.A 450 mg KCl/L Conducli"itY i°'\O ' 10"10 . -(µmhos/cm) \070 *i *..r_ .. .! " Tcmpernture (°C) "l.'\.<o "'l-..o "'\. .. \.a L."'\.lo "'\)\. ,_ pH (S.U.) 0.\S e,.05 l D O (mg/L) e.o '.}lb 1\-a "\!1 -:\..s 600 mg KCl/L Conductivity 13'10 . low . :* l320 .. 0 .. ' -. -'. -***' *Te m pera t u r e (°C) '1..."'.* (-"\.'\-& L."\*5 t.'-\.1 'bl.,_ p H (S.U.} 1-.e4 e.o5 e.02 '+.\10 D O (mg/L) eo 8.o "\;3 Q.Q 750 mg KCl/L Cond11c1ivity 161o . .. . ; .. --... . lSLIO 16&.J "'Tcmpernture (0 C) 1,"1,.. lc. 'Lt;.o .,_"' * '-i."\* 1 \.U.-S pH (5.U.) 't.02. 1-.l61. e.03 '+.5t1 DO (mg/L) Q.0 t3J:J /l.3 .}.3 900 mg KCUL Contl11c1i\

• ity 1.,. le>\O -. -. 1e3o le20 ... . . .. ... -..._..... .

.,.. " Temperntu r e (0 C) 1..L\. lo "l. .. \. -i'-\.'4 "'\."'\. p H (S.U.} g., ,?> 'l e&+ '+.Sf-D O (mg/L) A.I +.u 6.o 1 050 mg KCl/L Conducli\'il)' -. . -.. ';"( ... 1l .. ,

(µrnhos/cm} . . . -.. -*Temperature

(°C) 1.1.\."1 "\...*-\.'\ '"l.-!-'\ 1..1.\ * .., 1.c.\. 1."\. lo Init i a l Fi n al Initial F i nal I nitial Final *Temperatures performed

ll t he time oftest initiation , renewal or termination by the analyst identified in the Doily lnfonnnlion t a bl e l oc.-ited on Page I. ....... *nl in ity nnd hardnCEs performed by the ana lyst identified on the bench sheet specific for each ana l ysis and transcribed to thi s bench sheet by: t . ltn w iw \ Page 80of94 SOP AT21-Exhi bit AT21.I , revision 11-01-14

. I I 0 r _,_] [_ -l r.Q o I

• i l r* I 1-: ' :--Page 6 o f6 S p ec i es: Pime pl zales p r omelas PpKCICR Test Number: 25 Day {A naly s t identified for each day. perform e d pH , 0.0. and condu c tivity measurement s o nl y.) 3 4 5 6 Ana l yst Pf:..t , ...... (<.J IA-l.l.__ v---l)V jJ ,)v Conce n t r ation Paramete r I P 7' --.r. , ;!'-. r* *. ,,,.. .;.. _,..;..; ----" I --,.:;;;_*_...,_

,_ .* . pH (S.U.) Pu" ?,.oi "'} 0.\'5 "t.e2 'J.w DO (mg/L) -i. q ':\.:} ':l-lP ":).,A Conductivity -.. * . .., -: . -bZ.U 311-' 32'-{ . (lam hos/cm) *-_ -r.';r_ . --* . __......,.

.. : -.:J --*Alkalinity -.. -- --*-CONTROL . .. " . -. " (mg CaCO,!L) ... ...... ---_ . : *Hardness ' . --* v---g(o ; (m!!. C aCOJIL) .. .,;_ . ; .. -*Temperat ur e ""l!\.\o "\...\..-.\ "1.A. <a '""\. "\-lo .. "\... !>\. & (oC) pH (S.U.) M1-1.'15 ')..53 0.\0 ,r:;: ')-,\.( DO (mgfL) ?-.'1 J *<6 '\.0 /l..tl 14. \ -Conductivity , 300 mg KCl/L <0'4 \ '6'tt4 0&1 .. 0I05 >. (;t mhos/cm) -*Temperature \."\... r "\.4 .\a C..'l* 1 '\."\* "\..'-'*' '"1.'\.\o "\.-{. '\ "\.'\-1... c*q pH(S.U.) 611v.1 1.":.11.1 <(. \2-'l, Q" 'i-*62-DO (mgfL) +.r -:J A r:.\-.i-'l.ra (t.3 Conductivity ' 450 mg I CllL l 011) h C.O h'iO \HO {µmhos/can) -*Temperature ""\.'\,.lo '\.'\ .1 '""L'-\* \ l,.'{.\ "'-"l*1 \...'-\*L "l!V\ "1.'\*).. c*ci pH (S.U.) 0-05 "J .'1-'l <6. \ '2.-8.dl 'lM (JI 'l...sY DO (mgfL) f.q S . .J (Oft ').ca ".). t\ 600 mg KCl/L Condncli\'ity \320 ' i ' .. l3qo a'-( 2.0 {11mhos/cm) \ ---, -*Tempernture L_-.\. b '1..'-l-1 '"\..'-\* (.o L.'-\..1.... L.c.\.'\ '1.'\.-S ('C) pH (S.U.) e.os -:J.'1a '"fl.\' ':l-.50 'l , l./G\ DO (mg/L) e, o -:)._" i.o 't-d 00 \(1. 3 Conducti\*ity ., --750 mg KCl/L lS9J - - .. (µmhos/cm) . ' " Temperature l.t.\ ':"'\ '"\." l-1 '"cl.1 t.."\.S L."\. '\ ("C) pH (S.U.) 1-'l l e.oe /)..tJef 0.,\2.. "\.. DO (mg/L) 8 , l :} .-:J.. 4i *" I.DB q_(3 'l-5" 8.o Conductivity ,. -..

• 900 mg KCl/L t?bQ l.-o\D f 0a'}o

(µ111hus/c111) .. * ,._ . _ .. .:., -""' -.* ! *Tcmpernture 'l..'i* l ).'\ .'\ t."\. f '\."\."" "2..'\-1 \...'-\* 1.. (°C) pH (S.U.) T--:µ; 'l..'1-'\ 0.\2. 11..U P> DO (mgfL) B.I +.-+-j ,'2.-r.}.u 'b.l l.O.?. Conduclivity . -' 1050 mg KCl/L (1 l mhos/cm) Z\lcO 2-\G:lf) -.. Al'-f 0 ., < .... *Temperature lo 1...4* 1."\. "'\ (°Cl Initial F i na l Initial Final Initia l Fi n al Initial Final ** pcraturcs performed al Liie time oftest I nitiation , renewa l or 1erminmion by the urrnlyst idt:11ti1icd i 11 the Daily Renew a l Information t able l oca ted on Page I. inily and hardness performed by t he analyst identified on tile bench sheet specific for each analysis and transc r ibed to this bench sheet by: _ . Page 81 of 94 SOP A T21 -E x hibit AT2 I. I , revision l l-01-14 -u nJ z _, ......... 0.0 -"' N u > nJ "O I " Ceriodaphnia dubia Envlronmcmt411 Tostlng Solution s, , I n c. Chronic Reference Toxicant Control Chart Source: In-house Culture 1.08 1.06 1.04 1.15 1.10 1.05 1.00 Control Limits (+/- 2 Standard Deviations)

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• t t ... o t. ... t I o Warning Limits -**-**-**-*****-**-**-**-**-**-** . . . . . *-**-**----**-**-**-** **-**-**-**-** *-**-**-**-**-**-*** * * . ............................ , ............................................................. -*** " ....................

...... f. 4., ... Test date

• 7-day IC 25 = 25% i nhib i tion concentration. An estimation of the sodium chl orid e concentration which would cause a 25% reduction i n Cer i odaphnia reproduction (calculated using ToxCalc).

Central Tende ncy (mean logarithmic IC 25 oonverted to anti-logarithmic values) Control Lim i ts (mean logarithmic IC 25 +/- 2 standard deviations converted to anti-logarithmic va lu es) -.. -. Laboratory Warning Limits (mean lo garithmic IC 25 +/- 2 coefficent of var i ations converted to anti-logarithmi c v alues) *.**.*... USEPA Warn i ng Limits (mean logar ithmic IC 25 +/- 5 A.i o converted to anti-logar i thmic valu es, s A.i o = 10t h percentile of CVs reported nationally by USE PA) Page 82of94 CQ (I) co* W f: 1 wo : mnwn c.at T tt'""SI S o l 1,1 11 Df'l ,-, In c , 0 -(0 .,.,, Te s t number T es t dat e 1 01-12-16 2 02-09-1 6 3 02-09-16 4 03-08-16 5 04-0 5-16 6 04-0 5-16 7 05-10-16 8 06-07-1 6 9 07-12-16 1 0 08-02-16 11 09-13-16 1 2 10-04-16 13 11-01-16 1 4 12-06-16 15 01-10-17 1 6 02-07-17 1 7 03-07-17 1 8 03-07-17 1 9 04-04-17 20 05-02-17 7-d a y I C 25 T oxCa l De t e m 1l na tlo n 7-da y IC 25 (g/l N QI ....... et: 0 ..... 0 i:::: ... C1J ..... i:::: *u 0 :E u

15 offspring per surviv i ng female) USEPA Maximum Coefficient of Variation Guidance Criteria(< 42%), NCDEQ Maximum(< 40%) --* -.--. --.-...... --.. !,.. __ ----------!! ------------USE PA Lower PMSD Bound (>13%) USE P A Upper PMSD Bound Accep t ance Cr i teria(< 47%)

• Con tr o l Reproduction , Co effici e nt of V ar i a t i on (CV) or Perce nt M i n i m um S igni fi ca nt D i ffe r ence (PMSD) PMSD is the percent minimum significant differe n ce between the contro l a n d treatment that can be declared stat i stica ll y significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a m i n i mum acceptance criteria. C en t ra l Te nd e n c y (mean Control Reproduction , CV or PMSD) 9 5% Co n fid en ce Interva l (mean Control Reproduction, CV or PMSD +/- 2 Standard Deviations) 0 <o Control Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ToxCal D etermination Control R e p r oduction Control Reproduction CV Control Reproduction Test (offspring/female)

'"' Test num b er Test date Surv i val Mean CV MSO PMSO CT 95% Confidence Interva l CT 95% Confidence Interval CT '"' (offspring/female) (%) (%) CT* 25 CT+2S CT* 25 CT+2S 1 01-12-16 100 31.2 3.3 1.3860 4.4 31.6 28.6 34.6 4.4 2.1 6.8 5.8 2 02-0 9-16 100 31.6 7.0 1.9910 6.3 31.4 28.8 34.l 4.6 2.1 7.1 5.9 3 02-09-16 100 32.0 5.9 1.6300 5.1 31.3 29.0 33.7 4.7 2.1 7.2 5.8 4 03-08-16 100 29.4 5.4 1.7490 5.9 31.2 28.7 33.6 4.7 2.1 7.3 5.8 5 04--05-16 100 29.3 6.0 1.7920 6.1 31.l 28.6 33.6 4.8 2.1 7.4 5.7 6 04-05-16 1 00 32.3 4.9 1.6950 5.2 31.2 28.6 33.7 4.8 2.3 7.4 5.7 7 05-10-16 1 00 31.6 s.o 1.6360 5.2 31.2 2 8.8 33.7 4.8 2.3 7.4 5.6 8 06-07-16 100 27.9 2.6 1.7310 6.2 31.0 28.3 33.7 4.7 2.0 7.3 5.6 9 07-12-16 100 32.5 4.9 1.6710 5.1 31.0 28.3 33.7 4.7 2.1 7.3 5.6 10 08-02-1 6 100 29.9 6.6 2.1010 7.0 30.8 28.2 335 4.8 2.2 7.5 5.7 11 09*13-16 100 29.7 5.9 2.0410 6.9 30.7 28.2 33.3 4.8 2.2 7.5 5.7 12 10-04*16 100 28.9 3.4 1.6720 5.8 30.5 28.0 33.l 4.8 2.0 7.5 5.8 13 11-01-16 100 28.7 4.4 1.8440 6.4 30.4 27.8 33.0 4.7 2.0 7.5 5.8 14 12-06-16 100 29.3 5.1 1.4550 5.0 30.4 27.7 33.0 4.8 2.3 7.4 5.8 1 5 01-10-17 100 30.5 3.2 1.5240 5.0 30.4 27.7 33.0 4.8 2.3 7.4 5.8 16 02-07-17 100 27.9 8.5 1.8950 6.8 30.2 27.4 33.0 5.1 2.2 8.0 5.9 17 03-07-17 100 29.7 3.9 1.5300 5.2 30.2 27.4 33.0 5.1 2.2 8.0 5.8 18 03-07-17 100 29.9 5.3 1.91 80 6.4 3 0.2 27.4 33.l 5.1 2.2 8.0 S.9 l!:I 04-04-17 100 30.3 4.9 1.9240 6.3 30.2 27.4 32.9 5.1 2.2 7.9 5.8 20 05-02*17 100 31.0 5.9 1.8940 6.1 30.2 27.4 32.9 5.1 2.3 8.0 5.8 No t t!: Co nir o l Survival= USEPA minimum test acceptabi l hy 80% surv i va l. Con ol Reprod\lction = USEPA minimum Jest acceptability l5 offsprfng/surv1v1ng female. CV= Coeff i c i ent of va ri at i on fo r control reproduction. USEPA ma x l murr V gu i da n ce cr i teria (90'" pe r cent i le)< 42%. NCOEQ maximum CV acce ptan ce crlieria < 40%. M SD

• Minimum gnlllcant difference. PM SD Percem mln i mun significant diffe r ence. PMSO I s a meas1-1e of test precision. The P M SO 1s the minimum pNcent difference between the control and treatmen t t hat can b e dec l ared stali11i c ally significan t I n a whole effluent to xicity test. lower PMSD bound determi ned by USEPA (10'" percenlilel

> 13%. l ower PMSO bound re or e.sents a pracUc;il llmtt to sensit hti ty o f the lPs r me1hod n.o 1 a min1m11m .. r rltHia Upne r PMSD bound acceptance crlieria determined by USEFA (90'" percent il e)< C T Cen tr a l t ot he reproduc1ion , CV or PMSO values. S

• Standard deviatioo ol the re p rod u ction. CV or PMSO values. Te s t PMSO 1%) 95% Co n fiden ce Interval CT* 25 CT+ 25 43 7.4 4.4 7.4 4.3 7.3 4.3 7.3 4.5 7.0 4.4 6.9 4 , 4 6.9 4.4 6.9 4.4 6.9 4.3 7.1 4.2 7.2 4.3 7.2 4.3 7.3 4.3 7.3 4.3 7.3 4.4 7.4 4.3 7.4 4.3 7.4 4.3 7.3 4.3 7.3

. I c:-:::i . .-::: " -' Sodium C hlo r id e Ch r onic Reference To x icant Test (E P A-821-R-02-013 M e thod 1002.0) p e cies: C e riodaplz1 1 ia dubia Page 1 of6 C dNa C JCR #: 20 1 Dilution p re paration information

C o mmeTtt s: NaCl S t ock INSS number: INSS \S gc;, Stock preparation

I 00 g NaCl/L: Dissolve :50 2 N3CI in 500 mL Milli-Q water. Dil11tinn fll'Cf'I (m2/L) 600 800 1000 12 0 0 1400 Stock volume (ml) 9 1 2 15 IS 21 Diluent vol u me (ml) 149 1 1488 14 8 5 14 8 2 1479 volume (mL) 1500 1 500 1500 1500 1500 T es t onwni s m s our ce i 11for111nt io 11: Tes t i11for111nti o 11: Organism :ige: I < 24-h o u rs old Ran d omizi 11 11, template co l or: Daie and l i mes organ i sms we r e born I o'.'\S TI O'\C.O Incubator n umber and shetr between: location: 1.&\ Cu l ture board:

lS*\"'\ Replicate nu m ber: I ? J ; I

• I s I 6 I 7 f a I 9 I 10 YWT batch: Cullu r e board cup n u mber: "'!> "-\ I '"'\ I \'O I \ l° I \'\ f'\."\. l't._" J oc.\*'ll*

\"'\ Transfe r vessel infomia t ion: pH='"'\.l$$S.U. Tempera t ure= "\S. \ *c S e le11as1rnm batch: Os.{)\*I\ Average transfer vo lum e (mL): < 0.25 mL D"il Y r e n e ivnl i11f o rmatio11: D ay D:i t.e T e st ini tfat ion :ind feed i n g, MH S W A n: ll ys t rene w a l a nd fee din g, o r b:it c h used t ermination t im e 0 05-02-17 0°\\\ A ,rt I 05-03-17 o"\.*1.la*\'\ \J' ot\"L-\.-( 2 05-04-17 en'\ l"\ xc* 3 05-05-17 0 2 'U:> o..\*u.-11& 4 05-06-1 7 Ol"l.S l'i.<*n*n }( 5 05--07-17 o&7.:0 c 'St* Q'1. -\ "'\ xi" 6 05-08-17 o..t l \ X\ 7 , 09-1 7 Oil"'l.. -. Control inform.ntiou: Acce p ta n ce criteria Summm y o f t es t e ndp o int s: % o f Ma l e Ad ul t s: ()1. S20% 7-d ay LC 50 .., t'-too % A d ults h avi n g 3rd Bro ods: I 2: 80% N O EC (COO % Mortality:

01. s20% LO EC t-, /'\('\ Mean Offspring/F e ma l e:

<!: 1 5.0 offspri11g/fc m ale C hV tl'IC1.S. 5 %CV: <;:.'\ 1. <40.0% I C2s (OC.,'\. l Page 86of94 SOP AT14-Exhibit ATl4.I , revision 11-01-14 p C'"":J l, -p ' ,_. Species: Ceri odaplmia dubia CONTROL Da y I I Voung protlucctl (} Atlu l t mortn lity '-2 Young protlucctl 0 Atl ult morta l ity t_ 3 \'oung produced 0 Atlu!t mortnlity L 4 Young producctl Yo Adult mormli t y '--5 Young protlucctl l 't> Atlult mortalit y L 6 Young protl u ccd 0 Adult mortn l ity \_ 7 Young producctl \\., 2 () '--c '--0 '-c; '-0 "-Total )'Oung producctl '°?)"I;) Finni Adult Mortality '-'-X for J'd Brootls ')(_ ..,.,... Page 2 of6 CdNaC I CR #: 201 Survival aurl R ep roduction Dnta Replicate numb er 3 4 5 6 7 8 9 IO 0 r-i 0 0 0 () A (') L L '---'--'--'--'--'-() ("\ ri () 0 0 0 0 '-'--'-'-'-'-l_ '---() ("') b 0 0 0 0 0 '-\..-L '--L '--\...... L "-\ s "'.\ "-\ lo..\ '-{ s s '-'-'-L '-'-'--'--\ .,_ l\ V"t. "l. "l. \ ' I\ 10 '-"--L '-L L ""-'-0 0 0 0 u 0 0 0 \_. '-'---\..__. L '--\,.,.. '-\ c:f IS \'\ \"" \ \o \ \.. "?> '1. '--\.._. '-'-\._ '-'-)(._ 'X. r--")<_ '>£. v No t e: Adult live , D =dead), SB= split broocf (sin e l c hrnorl h e 1 w e cn t wo dnys}, CO= carry over (offspr i ng c a rried o v::r with adult during tran s fer). Co11 ce 11tration:

1. 600 muNaCl/L ,.., Surv i va l and Reproduction Data Replic a te num ber Day I 2 3 4 5 6 7 8 9 1 0 I Young produced 0 A 6 6 0 0 0 0 0 0 Adult mortality L \_ L '-L '-L. \.._. L \... 2 Young prol.luccd

("""I n a D (') b 0 a a 0 Adult mortality L. \_ '-'-'-'-\,_ \.._ '--'-J Young produced (') r> 0 0 b 0 0 0 0 0 Adult mortality '-'-\... '-\..-\... \... L \._ L .j Young produced 4 5 s s '"' s '-\ s s Atlult mortality '-\_ '-L L-L-\._ L \.__. L_ 5 Young protluccd 't) l.:. \0 ',_ \t_ IC '1... lb l \ \ \ A dult mortality L L \.._ L '-* '-L L L I._. G Young produced () 0i 0 (') 0 () 0 u ri ('") 1 dull morta li ty \._ '--'-'--L.. L L L. '-7 Young prod u ced ,5 >S 11 \'-\ l \c. \S I°& \\a Total young produced (.'\ "'2.C\ Finni Adult Mortality '-'\_. \.._ L \.._. '--'-'---\..__. \._ N ote: Ad ll lt mort n lit y (L =live , D =dead), SB= split brood (single brood split betw e en t wo days), CO* carry over (offspr i n g carried over w i th adult durin g transfer). Co11ce11lratio11: % Mortality: 01 Mean Offspring/Female

.3l. '2-% Reduction from Control: -o.to I. Page 87 of94 SOP AT 1 4 -E xhib i t ATl4. l , revis i on l l-Ol-l4 l r[=J --r;:::J Species: Cerio1/ap/111ia dubia 800 m!! NaCl/L Day I I Young produced () Adult mortality

'-2 Young produced ('1 Adult mortality L 3 Young produced l'J Adult mortality L 4 Young pr01luccd '-l :\dull morl:ilit)

• L 5 Young produced \ \. Adult mort:ilily l-G Young produced h Adult mortality

'--7 Young produced 2 0 \..... !'I '-0 '--::, \......_ '-r'-. '-"' Total young produced !:lo '-"' Finni Adult i\lorlality '--\.._ Page 3 of6 CdNaCICR #: 201 Survival mu/ Reprod11ctio11 Data Replicat e number 3 4 5 6 7 8 9 JO 0 D b D 0 0 (') 0 '-'--'--'-'--'--L b 0 0 0 0 0 0 0 '-\__ \__ '--'-'-L L 0 CJ {) t) {) 0 0 0 '-\.._. '-L-'--'---L.. '--5 s 5 "-\ "-\ '-\ "" '-'---'--'-\._. '--'--\........ \\ tD \0 l '?> \ '1.. \ "L ,,_ '-L '--'-'-'-'-'-("") {') () 0 0 0 0 0 \._ \_ '-'---'-\..... L L.. \ i \ \.\ ,5 \::, ' CS) ,, \ '-'"l.. '\ '-'--'-'--'----N ote: Ad11Jr monnlit y CL= liv e, D =dead), SB= split brood (single b r ood split bctwe e n two days). CO= carry over (offspring over w ith ad11ll d11 r ing l rnnsfcr). C011ce11tratio11: % Mortality: 01. Mean Offso r ing/Female

% Reduction from Control: 1000 mg NaCI/L Survival mu/ Reproductio11 Data -Replicate number Dny l 3 4 5 6 7 8 9 10 I \loung producctl 0 (\ D 0 n 0 0 0 0 0 Adnll moriali ry \.._. L L L L.. L '-L '-'-2 \'oung protluccd (") 0 () 0 0 (") () a 0 0 A dull mortality '-L L L L.. L L \....._ '-L.. 3 Young protluccd (} 0 0 {") 0 0 0 0 0 0 Atluh mortality \...-\.... '-'-'---\_ \..._ L '--L 4 Young producctl '"'\ s '.\ ::, '-\ "--\ '-\ \..\ '-l Adult mortality L--\..-L '-'--'-L-L \...-'---5 Young prollucetl l\ l\ 10 n ...... \\ '"L \-,_ 1\ '--AtluJt morrnlity '--'-L '--'--L \.... \..... L \... G Young producctl n f) (') (', 0 0 0 0 0 0 Atlul! mu rl:ility \... \.-'\_. '-'--L \..... '-'--\.... 7 Young pro.ducc tl \ :!> \ L. \S \ <; \ \... 13 'S Tot.a l young procluccd t.% "tJi L.'\ u .,_"' 3C finnl ,\du ll Mortality L-'--'--L L-\.... \_ \....._ \....._ L N ot e: Aclu l l monalily (L = live , D =dead), SB = splil brood (single brood s p l it b e twe e n two days). CO= ca rry over (offsprin g c n rriecl over with adull durin g transfer). Co11ce11tratio11: % Mortalitv: % Reduction from Cont rol: s. i 1. SOP ATl4 -Exhibit ATl4. I. revision 11-01-14 Page 88of94 l Pag e 4 of6 Species: Cer i o dap lmi a d ubia CdNaCICR #: 201 1200 mg NaCl/L Su r viva l n 11 d Re p rod 11 ctio 11 Data Replicate number Day I 2 3 4 5 6 7 8 9 JO I \loung produced () b 0 0 0 () 0 0 0 (') Adult mortality \..... '-'--'-'--'--L \..._ \._ '-2 Young produced 0 a 0 n 0 0 ('\ 0 a 0 *I Adult mortality '-'-'-\.... '-'-'-'-'-'-3 Young produced 0 0 a 0 ri 0 ri {\ 0 C"I Adult morta l ity \.._ '-L.. '-'--'-L '-'-\._ 4 \'oung produced .,_ ,_ ,_ '-\ '-\ 'b '--\ Adult mortality \.._ \...... '-L.. '-'-'-'-\._ "'--5 Young produced ".\ lD c;. s IS 5 \.. '"\ Adult m orlality '--'-\... '-\.... '-'--'--'--\.... G Young produced D 0 0 0 0 0 D 0 0 D Adult mortality \.... \.... '-\... L-\..._. '-'--L L 7 Young produced .$ \ lo s L.. '4 .'.!> \o \..\ Totnl young produced \ "'L " \ '4. l \\g \1. \ 1.. \ 1. \S Final Adult Mortality '--'--'--'-'-'--'-'--'-\.._ Note: Adult mortality CL= live. D =dead}, SB= split brood (single bro od split between two days). carry over (offspring cnrric d over with adult durin; transfer). Co 11 ce11tratio11: % Mo1tality: 0 1. / Mean Offspring/Female: \1..'L % Reduction from Control: (o () .\r:..,. 1400 mg NaCl/L Survival mul Reprorl11ctio11 Data Replicate number Day I 2 3 4 5 G 7 8 9 10 l \'oung produced () 0 0 0 0 0 () 0 0 n Adult mortality L L L.. '--'-L '--L '-l 2 Young produced 0 0 (') ("\ ri (\ 0 0 a Adult mortalil)' '-L L L '-L. \....... L '---'-3 Young produced ll (\ 0 D 0 (', 0 0 CJ () Adult rnortnlity L \._ '-'--L. L '-L L \........ 4 Young produced \ L... "'L \ \ ""l. Adult mortality L-'-\._ '-'-\....... L '-'-L. 5 Young produced 0 a 0 '-0 \ ("') 0 ("\ 0 Adult mortnli t y \..._ \.._. '-'-'-L \.... '-'-'-6 Young produced 0 f'I () (') b 0 0 ,_ 0 0 Adult mortality '-'-L-'-\...... '---'-L-L-'--7 Young produced "'l.. 0 <::> () 0 D \ () 0 () Totnl yo ung produced L. ..,_ to L. "b s Final Adult Mortality \.._.. L-'-'-L-'-L \....... '-Note: Adult mortality (L =liv e, D deod}, SB= sp lit brood (single brood split between two days), CO* carry over (offspring carrie d over witl1 a dull during transfer). Co11ce11tratio11: % Mortality: 0 1. Mean Offspring/Female

% Reduction from Co ntrol: 8'1.11. Page 89 of94 SOP AT14-Ex.hibit ATl4.l , revision I l*OT-14 (Q (I) co Q 0 .... co '\ Env i ronm e ntal Testing So l utions In c. C o n t ro l D ay 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 5 4 5 1 0 13 12 6 0 0 0 7 16 15 12 T o ta l 30 33 34 600 mg N a Cl/L -D ay 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 5 s 5 10 13 10 6 0 0 0 7 15 1 5 l l T o t a l 2 9 33 32 800 m g N a Cl/L Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 3 5 5 11 12 1l 6 0 0 0 7 15 14 lL Total 3 0 2 9 30 R ep li cate numbe r 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 4 4 4 11 12 12 12 0 0 0 0 15 14 16 13 31 3 0 32 29 Re p lica t e n u m b er 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 4 5 4 12 1 2 10 12 0 0 0 0 13 14 16 15 30 30 31 31 Re p l i c ate n umber 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 4 5 4 10 10 13 1 2 0 0 0 0 18 14 1 5 13 33 28 33 29 Verificati on o f Ce r i odaph n ia R e pr o d uc t i on T ot al s 1000 mg N a Cl/L -T o ta l 8 9 10 Da y Rep l i cat e nu mb er T otal 1 2 3 4 5 6 7 8 9 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 s 44 4 4 5 4 3 3 4 4 4 4 4 39 11 11 10 114 5 ll 11 10 12 10 11 12 12 11 12 112 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 l3 16 16 152 7 13 12 15 15 15 16 13 14 13 15 1 4 1 28 32 3 1 310 T o tal 28 2 8 29 30 28 31 29 30 28 31 292 1200 mg NaCl/L Total 8 9 1 0 Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 0 I) 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 5 ; 6 48 4 3 2 2 4 2 4 4 3 3 4 31 13 11 11 114 5 4 6 5 5 3 8 5 6 3 7 52 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 16 13 16 150 7 5 1 6 5 2 4 3 3 6 4 39 3 4 :2 9 3 J 3 12 T o tal 12 9 13 1 4 7 16 12 1 2 12 15 122 1 400 mg NaCl/L -T o tal 8 9 1 0 D ay Rep li ca t e number T o tal 1 2 3 4 s 6 7 8 9 10 0 J 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 J 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 42 4 l 2 2 1 3 l 2 3 3 3 21 12 1 2 13 116 5 0 0 0 2 0 1 0 0 0 0 3 0 :J 0 0 6 0 0 0 0 3 0 0 2 0 0 5 15 1 7 16 151 7 2 0 0 0 0 0 1 0 0 0 3 --31 3 3 33 3 0 9 To tal 3 2 2 3 6 2 3 5 3 3 3 2 .......... *r E nvironmental Tes ting S o l .. tlo n s. Inc. Concentration (mg/l NaCl) l 2 Control 30 33 5 00 29 33 8 00 30 29 1000 28 28 1200 12 9 1 400 3 2 Ounn ett's MSO value: 1.894 PMSO: 6.1 3 34 32 30 29 13 I. 4 3 1 30 33 30 14 3 MSD = Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Replicate number Surv iva l 5 6 7 8 9 10 (%) 30 32 29 28 32 31 100 30 31 31 34 29 33 100 28 33 29 31 33 33 1 00 28 31 29 30 28 31 100 7 16 1 2 1 2 12 15 100 6 2 3 5 3 3 100 M i nimum Significant D i fference Percent Mi nimum Significant Difference Test num be r: CdN aCIC R 1#201 T est dates: May 02-09, 2017 Averag e reproducti on Co effi c ie n t of Percent reduction (offspring/female) variation (%) from control (%) 31.0 S.9 Not applicable 31.2 S.6 -0.6 30.9 6.4 0.3 29.2 4.2 5.8 12.2 21.8 60.6 3.2 41.1 89.7 PMSO is a mea s u re of test prec i sion. The PMSO i> t he minimum percenl difference between t he control and treatment that can be declared statist i cally significant i n a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10'h i;ercentile) = 13%. Upper PMSO bound determined by USE PA (90 1 h percenti le)= 47%. Lower and upper PMSO bounds were determined from th e 10 t h and 90th perc ent I , r espectively, of PMSD data from EPA's WET ln t erlaboratory Va r iability Study (USE PA , 200la; USE PA , 200lb). The lo wer PMSO bound re pr e sents pr ac tical limit 10 the sensitivity of the tes t method and Is not a minim u m cceotance criteria. IJ<;f PA 7001 11 , 2 00lb. Report: lnte r liboritory Va r lab lllty Study of EPA Sho r t-t erm Chronic Atutc Whole Eff l u e nt Toxicity Tes 1 Ml!thods, Voluml!s 1 an d 2*AppendD:. [PA-821-8-01-004 and EPA-821-11-01-005. US Envir onment a l P rotection ARe ncv , Ci nc i nn a t i , OH. Statistica l Analyses tnwlro nm*fftal THtlnQ SoluUon l. IM. Cer i odaehnia Surv ival a nd Reeroduction Test-Reeroduction Start D a t e: 5/212017 Tes t ID: CdNaCICR Sample ID: R E F-R ef Toxican t End Date: 51912017 L ab ID. ET S-E n v ir Te s t i ng So l. Samp l e Type: NAC L-S od 1 u m chloride Sample Da t e. Protocol* FWCHR-EPA*621

• R*02*0 1 3 Test Specie CD-Cer l odap h nia dvbia Commen ts: Conc-m5 1 L 2 3 " 5 6 7 6 9 10 D-Conlrol 30.000 33.000 34.000 31.000 30.000 32.000 29.000 26.000 32.000 31 000 600 2.9 000 33.000 3 2.000 30.000 30.000 3 1 000 31.000 34.000 29.000 33000 600 30.000 29.000 30.000 33.000 26.000 33 000 29 000 31 000 33.000 33.000 1000 26000 2 6.000 29000 30.000 26.000 3 1.000 2 9.000 3 0.000 2 6.000 31 000 12.0 0 1 2 0 00 90 0 0 1 3.000 1 4.000 7 000 15.000 12 000
• 2.000 12.000 1 5 000 14 0 0 3.000 2.0 0 0 2.000 3.000 6.000 2000 3.000 5 000 3000 3.000 Transform: Untransformed 1-Tailed Isotonic Conc-ma1L Mean N-Mean Mean Min MaJC CV% N !*Stat Critical MSD O.Conlrol Jl 000 1.0000 'H.000 34 onn 5 8 11!'1 10 600 3 1.200 1 0065 31.200 29.000 34.000 5 , 613 10 *0.241 2.267 1.894 800 30.900 0 9 968 30 900 28.000 33.000 6.373 10 0.12 1 2.267 1.694 1 000 29.200 0.9419 29.200 26.000 3 1.000 4.210 10 2.173 2.267 1.894 *1200 1 2.200 0.3 935 12 200 7.000 16.000 21.7 9 0 10 22.694 2.26 7 1.894 "1 40 0 3200 0.1032 3.200 2.000 6.000 41.143 10 33.557 2.287 1.894 A uxilia!}'.

Tests Statistic Cr lll cal Kolmogo r ov D Test ind i cates normal dist ri bu ti on (p > 0.01) 1.00629 1.035 Bartlelt's Tes t tnd1ca les ve ri an c es !E = 0.23! 6.9004 4 15.0663 Test (1 *tall. 0.05) NOEC LO EC ChV TU MSDu M S Op MSB MSE Dunnett's Test 1000 120 0 1 095 45 1.89434 0.061 1 1 1 481.5 1 3.4 3 1 46 Treatments vs 0-Contro l Linear Interpolation (200 Resample!O Point mg t L so IC05 959.412 63. 1 30 6 IC10 1014 24 8 4 1 223 IC15 1032.53 5.7 1 3 1 3 JC 2 0 1050.82 5.39734 l C25 1069.12 5.25268 I C 40 1124 5.8972 I CSO 11 60.59 7 02907 95 0/o Cl 779.216 005.53 999 237 1 023.54 1018.69 1041.09 1036.27 1056.92 105 7 36 1 0 77.82 1110.95 11 3 5.01 1 1<15 4 1173.15 Skew -1.2229 -3.623 7 .() 075 0 -0.019 1 0.0038 *0.0 7 15 -0.0600 1.0 0.9 0.8 0.7 "' 0 6 .,, o.s 04 "' er:: 0.3 02 0 1 0 0 c.-----<1-.(Y"" Mean 31.100 31.100 30.900 29.200 12.200 3.200 Skew -0.1735 F-Prob 3.2E-42 0 1 40 35 30 g 25 u ::> 15 20 c. 15 10 5 0 c. 0 u 0 Page 92of94 0 Dose-Response Plot 500 1000 Dose mg/L 1-lai l, 0.05 le v e l of s i gnificance 1500 N-Mean 1.0000 1.0000 0.9936 0.9369 0.3923 0 1 029 Kurt 0.13786 df 5 , 54 -... ..__., l j J P a ge 5 o f 6 S p e cie s: Ce riotlaplmi a dubi a CdNaC I C R #: 20 1 Da ily C lt em i st 1 y: Day (Ana l y st i d e nt ified fo r ea c h da y. p erformed. p H. 0.0. and co ndu cti vit y m easurem ent s onl y.) 0 l 2 Ana l)*st """' II-J-.1 .L .;..., C on ce n trat i on Pa r a meter --r .*, -. . pH (S.U.) 8.2.l S.*o 'b.02. e.oe DO (111g/L) ")._q "lr Conductivit y . ---,32..1 3'2<3 !" (µmhos/c m) -*-l --* -,_, "" .. CONTR O L *Al k alinit y jl' ---.;, lo\ * *.. <j* la"'Z.

• l ' '* ... *-* ... *lln r d n c ss <(g g-i. "" --(m g C aC Oi/Ll *' I . (. " Temperat u re (0 C) L."\.lo l.C:.. \ "'-\.\."'\ 1...&.\.E

"'l.'\. 8 "'\.'\."\ pH (S.U.) t)) 1<; e.o'-1 DO {m g/L) "1..8 '\...+ 1'-8 600 mg N aC l/L C o nducth*i ty l?AO (µmh os/cm) . : " Te m pernture (°C) &.'-\* ""'\ 1..\..\-"'\.'-\*1 U\*"l pH (S.U.) 0.\"' A.01. "iO.-s t!>Cz. 'H\\ DO (mg/L) '}.0 '.:\+. -:l':\-1-.q 8 0 0 m g NaCl/L C on d uctivity l1=tQ l":t-0 11-t10 (1 m1ho s/em) ' -" Tc m pernturc (°C) '1.."-\* \ "\S.O "\_'-\. i ."\ 1..'-l* 1. "l.'\.t pH (S.U.) eJ . .\3 e>.oZ '-tAlO A.oz. 1:\..*.(3 D O (mg/L l ":l: a l.},A ":\,.q 1:.e'J 't.'l 10 00 mg NaCl/L C onductivity " , . "' ,_ (µmho s/c m) 2.\30 '-.000 '* -*Temperature (0 C) '1..'-1.* 1 "\.J. * .i "l'\.* g 'N."\ -i.'-\* 1 L.'\.f pH (S.U.) e.,z.. '+.<:\( eoa e.oz.. 4.01.P DO (m g.IL) r.}a 1200 mg NaCl/L Con<luc t i v lt y -(umho s/cm) a4so , " Tempernt u rc (°C) l.."\.1 1.'-\. f "'\.'\ * .f -i.s.o 1.'-1.*'\ 1!-i. t pH (S.U.) 0.12-6.01 't.e"' DO (mg/L) e>.o 0o 8.o '+<'.\' 14 0 0 mg NaCl/L °'1'10 IA 'l 1<J (µmho s/cm) \ . *Temperature (°C) '"'l"\*1 "1.-.\.. f i.t.1.. e '\ "l '{." Init i al F in a l Init i al F in a l Ini tia l F i na l *T e m pera tur es pcr fo n n c d at the t im e of t est i ni tiation , r e ne w a l or t c rminm ion b y the a n a l yst id c n t i!ic d in th e D ai l y R e n ew al lnforn ia tio'l ta b lc l oca ted on P age I. A l ka lini ty a n d pc rfo n n c d b y t h e ana l ys t id cn tili c<l on t he b e n c h s h eet s pecifi c for cnch a nalysis and t ra n scribe d Lo th is be n ch s h eet b y: . Page 93 of94 SO P A T l4-E x h i b itATl4.!, r e vision 11-0 1-1 4 'j I I .. I l l 1 Page 6 of6 J -Species: Ceriodaplmia dubia CdNaCICR #: 2 0 1 Day (Ana l yst identified for each day , performed pH, D.O. a nd conductivity measurements only.) 3 4 5 Ana l yst µ Q..::. """' °"" ...... .J.--I)..., lr' .u Concentrat ion Parameter " -. !'!. * --" . _:_ pH (S.U.) 0.\3 ts J'S e.uo. e.\l\ DO (mg/L) +_-:}-+.'l ';/, Ci. 1-.6 r..a.,. ll'l Co111Juctivity . -32'-l . 3ZD ;;Tu .31-:r-(um ho s/cm) -.. -. -*A l kalinity -----* -* --. CONTROL .. CaCO ,IL) -* <1l ' . -*nanlne.ss -. . 86 .. **-' .. -, . ,. (mg CaCO;IL) --*-"Te mperature L.."'-\.l '"l.S.'L. '\ "l.."\ ""1..1.\*1 '1.S*\ 1."\.& c 0 c> pH (S.U.) A.OS +.Ar '\Al a.o-6" 0,0\ e,.\\.\ DO (mg/L) 1-.'l -:i.. 'l 1-6 /.\.ll Co n<lu crivity -600 mg NaCl/L l'260 14\0 ' *400 .. (µmhos/cm) --*Temperature '1..S.1.. Vo\!\ "\S..O 1.."'.*1 . .& ).i.\.'=\ c 0 c> pH (S.U.) 0.0-; i e.o2 0.\3 DO (mg/L) r-l-.'1 'l, , q Con<lucrivity 800 mg NaCl/L 1'1*30 l1":20 1150 (umhos/cm) --"'Temperature '1..'-\ 1..s.o \S.o '1.S-0 '1.."\* 1 '\.."\..(I-C"C) pH (S.U.) e.03 "'6.0111 /f?\ I 0.0lP Af\l (2),\3 DO (mg/L) ,\ /}J:} I'.\ e ".\-.q '1-.0 1000 mg NaCl/L Conductivity " ;; C 1 rn1ho s/c m) a\OO . -.. --" "'Tempera tu re '1.c.( * .f -v\. '\ LS.o l,S.Q 1..-\ .f l.."l.'\ "'l."(. *' c*ci pH (S.U.) ,q.02. +.C\<i. "&.o.., 4!10 '+-.C\Gl 0.l3 DO (myL) e.o "i :1... 'lSo E!).D '-t , q C n<lucti v ity -1200 mg NaCl/L ti430 .., .;\%t> -a'{(t)O "". "* *Temper:itu r c \ "1$.0 'lo\ *4' 'lS-1.. °l."l*'i ("C) pH (S.U.) . .tn. 0.05 6.IY'> DO (mg/L) +.JI( e?..V a .* Con<l ucth<ity -. 1400 mg NaCllL )i1-1o ;;\'lo.;:, A"i'10 , ., ?.6 10 (11mhos/cm) -. . --: -"' " Temperature 1."l.1 g is.o 1...'i 1.'-l-'\ 1,."\ ... '( 1.'-\.<\ (°C) In i t i a l Fi nal Initial Fi n a l Initial Final Initial "'T emperatures performed at th e time of test initi a tion , renewal or 1crminn1i on by the analyst identified in t he D a ily Renewa l lnfomm tion table located on Page I. Alkalinity and hardness performed by the onal)'st identified on the bench sheet spcci fie for each ana l ys is and transcribed to this b enc h s heet b y: Page 94 of94 SOP AT1 4 -Exhibit AT 14.1 , revision I 1-0 1-1 4 6 ,,_ ---. -A.\3 t.A ,-::. J -.. . - ' -"\.'$. t> e.o3 '\-. c=t '-*-'. . "'l.S.. () e.1)2... '} * .::t. ,I -=-,_ .. \. g,o . " . . "'2,.'{. & e.o * "t :"I . _ .. '1...\. '\ 'lAe Q*O -.. -; --' L.'S. 0 Final}}