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{{#Wiki_filter:Tennessee Va ll ey Aut hority , Post Office Box 2000 , So dd y Daisy, Te nne ss ee 37384-2000 June 8 , 2017 Chattanooga Environmental Field Office D i vision of Water Pollution Control 1301 Riverfront Parkway , #206 Chattanooga , Tennessee 37402-2013 TENNESSEE VALLEY AUTHORITY (TVA) -SEQUOYAH NUCLEAR PLANT (SQN) -NPDES PERMIT NO. TN0026450  
{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 8, 2017 Chattanooga Environmental Field Office Division of Water Pollution Control 1301 Riverfront Parkway , #206 Chattanooga, Tennessee 37402-2013 TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR May 2017 Enclosed is the May 2017 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period . The final report for toxicity testing conducted at outfall 101 ,
-DISCHARGE MONITORING REPORT (DMR) FOR May 2017 Enclosed i s the May 2017 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period. The final report for toxic i ty testing conducted at outfall 101 , April 30 -May 5 , 2017 , is enclosed. No adverse effects were observed. If you have any quest i ons or need add i t i onal information, p l ease contact Millicent Garland by email at mrmoore@tva
April 30 - May 5, 2017, is enclosed . No adverse effects were observed . If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714 .
.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the informat ion , the information submitted is , to the best of my knowledge and bel i ef, true , accurate , and comple te. I am aware that there are significant penalties for submitting false information, including the possib ility of fine and impr isonment for know i ng viola ti ons. ony L. W i lliams Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosu r es): U.S. Nuclear Regulatory Commiss i on Attn: Document Control Desk Washington , DC 20555 P ERM ITTEE NA M E/AD D R E SS (Include Facil i ty Name/L ocati o n if Di ff erent) Na me _
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
___ _ Addre s.L
ony L. W illiams Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosu res) :
___________
U.S. Nuclear Regulatory Commission Attn : Document Control Desk Washington , DC 20555
_ ___ _il NTER OFFICE OPS-5 N-SQ!!l _______ _ _ _ _ __§O D DY -DAISY .._I.N_273B4_ _______ _ Fa c J!!ly _ _I VA-_fil:QUOYA H NU CL EAR P LAN T _____ _ L o c a t i o.!]_ _l!AMIL TOJi.C OUNTL_ _________
 
_ A TI N: M illic e nt Gar l a n d NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NP DESJ DISCHARGE MONITORING REPORT (O M R) TN0026450 10 1 G PERMIT NUMBER DISCHARGE NUMBER AR M O D YE MO DAY 1 MAJOR (SUBR 0 1) F -FINAL D I FFUSER DISCHARGE EFFLUENT *** NO DISCHARGE D ... Form A ppr o ved. OMB No. 2 040-0004 F r om ;7 I 05 01 : To [ 17 : 05 I J i NOTE: Re ad i nstruc t i on s b e fore completinQ thi s f o rm. PARAMETER i>< QUANTITY OR L OADING QUALITY OR CONCENTRATION NO. FREQU EN CY SAMPLE EX OF TYPE AV ERAGE MAXIM U M U N IT S MI NIMUM AVERAG E MAXIMUM UN I TS A N A L YSI S TEMPERATURE, WATER DEG. S AMPL E ******** ******** ******** ******** 35.6 0 31 / 31 RCORDR ** 04 CENTIGRADE M E ASUREMEN T 00010 1 0 PERM IT ******** ******** **** ******** ******** Req. Mon. DEG.C. CONT I CALCTD EFFLUENT GROSS R E QU I REMENT DAILY MAX NUOUS TEMPERATURE , WATER DEG. SAMPL E ******** ******** ******** ********
PERMITTEE NAME/AD DRESS            (Include Facility Name/Location if Different)                         NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ                  MAJOR                                    Form Approved .
23.4 0 3 1 I 31 MODELO ** 04 CENTIGRADE MEA SUREMEN T 00010 z 0 PERMIT ******** ******** **** ******** ******** 30.5 DEG.C . CONTI CALCTD INSTREAM MON I TORING REQUIREMENT DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & S AMP LE ******** ******** ******** ******** 1.6 0 31 / 31 CALCTD .. 04 UPSTRM DEG.C M E ASUREME NT 000 1 6 1 s P E RMIT ******** ******** .... ******** ******** 3.0 DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** ******** 0 31 I 31 RCORDR /908 03 .. TREATMENT PLANT M EA SUREM E N T 50050 1 0 PERMIT ******it* Req. Mon. MGD ******** ******** ******** **** CONTI RCORDR EFF L UENT GROSS R E QU I REMENT DAILY MAX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1 433 ******** ******** ********  
Name _ _!VA~EQUOYA~UCLEARPLANT _ _ _ _                                                                               DISCHARGE MONITORING REPORT (OMR)                                                                    OMB No. 2040-0004 (SUBR 0 1)
******** 0 31 I 31 CALCTD TREATMENT PLANT M E ASUREMENT 03 03 50050 1 0 PERM I T Req. Mon. ******** MGD ******** ******** ******** MGD CONTI CALCTD EFF L UENT GROSS VALUE R E QUIREMENT MO AVG NUOUS CHLORINE , TOTAL RESIDUAL SAMPL E ******** ******** ******** 0.018 0.036 0 20 / 31 GRAB MEASUREMEN T .. 19 50060 1 0 PERMIT ******** ******** .... ******** 0.1 0.1 MG/L FIVE PER CALCTD EFFLUENT GROSS VALUE R E QUIREMEN T MO AVG DAILY MAX WEEK TEMPERATURE  
Addres.L _f. ~BO X.1QOO _ _ _ _ _ _ _ _ _ _ _ _
-C, RATE OF SAMPLE ******** 0.2 ******** ******** 0 31/31 CALCTD 62 .. CHANGE M E ASUREMENT 82234 1 0 PERM I T ******** 2.0 DEG ******** ******** ******* **** CONTI CALCTD EFFLUENT GROSS R E QUIREMENT C/HR _,/" -----)--------*-
_ _ _ _ilNTEROFFICE OPS-5N-SQ!!l _ _ _ _ _ _ _ _                                                                    TN0026450                                     101 G           F - FINAL
NUOUS DAILY MX *-/ NAME/TIT LE PR I NC I PA L E XECU TI V E OF FI CER ' ''""'""" ,.,,.,," ,_ '"" '"'' """"'"' '"' ., "'""--""'"' "&#xa5;. _/ TELEPHONE D A TE d i rec ti on or superv ision in accordance with a system des i gned lo assure thal qua li fied Anthony L. W illi ams personnel proper ly galher and evaluate the in format ion submilled. Based on my i nquiry o the P r e:d e n t p erso n or persons who manage the system , or those persons dire ctly r esponsible for gal er ing lhe info rmat ion , the in f orma l io n subm i lled is , to th e best of my knowledge and belief , true , 423 8 43-7 0 01 17 06 0 7 S ite V ice President accurate , and complele. I am aware th at there are significant pena l t i es for submi tl i ng fa lse SIGNATURE O F PRINCIPAL EXECUTIVE I infor mation , i nc luding the possibili ty of fine and i mpr i sonment f or knowing v iol ations. OFFIC ER O R AUTHO R IZED A GE N T AREA I NUMBER YE AR MO D A Y TY PED O R P R I N TED CO DE CO MM E N TS AND E X P L ANA TIO N O F ANY VIO LAT I O NS (R e f e r e n ce a ll a tt ac h me nts he r e) No c lose d m o d e operation. T h e following injecti ons o ccu r red: Spectrus CT 13 00 (max calc. was 0.0 39 mg/ml, limit 0.0 5 mg/L), Spe c t rus BO 15 00 (max ca lc. was 0.046 m g fml, limit -2.0 mg/ml). EPA For m 3320-1 (R EV 3199) Previous editions ma y be used Pf!O P. 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if D i fferen t)
_ _ _ __&sect;ODDY - DAISY.._I.N_273B4_ _ _ _ _ _ _ _ _                                                                PERMIT NUMBER                      DISCHARGE NUMBER            D IFFUSER DISCHARGE FacJ!!ly_ _IVA-_fil:QUOYAH NUCL EAR PLANT_ _ _ _ _ _
___ _ Addres_L
                                                                                                                  ~
------------___ .J!NTEROFFICE OPS-5N-SQNl _______ _ NATIONAL POLLUTANT DIS C HARGE ELIMINATION SYS TE M (NPDES J DISCHARGE MONITORING REPORT (DMR) TN0026450 101 T MAJOR (SUBR 01) F -FINAL Form Approved. OMB No. 2040-0004
I 05 r~RING                  PE~t Locatio.!]_ _l!AMIL TOJi.COUNTL_ _ _ _ _ _ _ _ _ _ _                                                                                                                              EFFLUENT D                                                                          D ...
_ _ _ _&sect;ODDY -DAISY J.N_..]7381._
17 : 05 I Ji AR      MO                          YE      MO      DAY
_______ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Fac_ill!y_
                                                                                                                                                                                      ***  NO DISCHARGE i><
..JYA-SEQUOYAH NUCLEAR PLANT _____ _ LocatiO.!L
A TIN:M illice nt Garla nd                                                                            From ; 7                        01 :        To [                      1 NOTE : Read instructions before completinQ this form .
_!!AMIL TOJi.COUNTY._
PARAMETER                                                                QUANTITY OR LOADING                                                  QUALITY OR CONCENTRATION                                 NO. FREQUEN CY SAMPLE EX       OF           TYPE ANALYSIS AVERAGE                    MAXIMUM              UNITS              MINIMUM            AVERAGE              MAXIMUM             UNITS TEMPERATURE, WATER DEG.                               SAMPLE                                              ********                                 ********           ********                                                  31 / 31      RCORDR
_________
                                                                                ********                                           **                                                         35.6                 04      0 CENTIGRADE                                       MEASUREMENT 00010       1     0                                 PERMIT REQU IREMENT
_ ATIN: Millicent Garland PARAMETER
                                                                                ********                   ********               ****             ********           ********         Req. Mon.             DEG.C.             CONTI        CALCTD EFFLUENT GROSS                                                                                                                                                                         DAILY MAX                               NUOUS TEMPERATURE , WATER DEG.                             SAMPLE                    ********                   ********                               ********           ********               23.4                         0     31 I 31     MODELO CENTIGRADE                                      MEASUREMENT                                                                      **                                                                             04 00010       z     0                                 PERMIT                   ********                   ********               ****             ********           ********               30.5           DEG.C .             CONTI         CALCTD REQUIREMENT INSTREAM MONITORING                                                                                                                                                                      DAILY MX                                 NUOUS TEMP. DIFF. BETWEEN SAMP.               &           SAMPLE MEASUREME NT
>< QUANTITY OR LOADING AVERAGE MAXIMUM IC25 STATRE 7DAY CHR SAMPLE ******** ******** CERIODAPHNIA MEASUREMENT TRP38 1 0 PERMIT ******** ******** EFFLUENT GROSS REQUIREMENT IC25 STATRE 7DAY CHR SAMPLE ******** ******** PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT ******** ******** EFFLUENT GROSS REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT UNITS MINIMUM ** >100.0 .... 42.8 MINIMUM ** >100.0 .... 42.8 MIMINUM DAY 31 EFFLUENT NO DISCHARGE D ... NOTE: Read i nstructions before complet i ni:i th i s fo r m. QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS ******** ******** 0 1/180 COMPOS 23 ******** ********
                                                                                ********                   ********               ..              ********           ********               1.6               04        0     31 / 31       CALCTD
PERCENT SEMI COMPOS ANNUAL ******** ******** 0 1/180 COMPOS 23 ******** ******** PERCENT SEMI COMPOS ANNUAL REQUIREMENT  
                                                                                                                                  ....
/ _) .. --------_.
UPSTRM DEG.C 000 16      1     s                                 PERMIT
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I C e rt i fy under pena l l y o f l a w tha t this d oc ument an d all a tt a c hm en ts were prepare d di r ection or s u p ervi s ion i n a cco r da n ce w ith a s yst em d esigne d to assure tha t qu a lifie d Anthony L. Williams p er s onn e l p ro p e r ly ga t her an d ev a lu at e th e in f or m ation submi tt ed. Based o n my i n qu i ry t he p e rs o n or p e r s o ns who ma n age t he system , or tho s e p erso ns d i r ectl y r es p o n s i b le f or g h a r ing the i nf o r matio n , the i nformation submi tt e d i s . to the best o f my knowledge and bel i ef , Site Vice President accurate , a nd complete. I am aware that there are significant pena l t i es for subm i tt i ng f alse i n f ormation, i nclud i ng the possibility of f i ne and i mpr i sonment for knowi n g v i olat io ns. T Y PED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLAT I ONS (Referen c e all attachments here) Toxicity was sampled April 30 -May 5 , 2017. EPA Form 3320-1 (REV 3/99) P r e vious edi t io ns m a y be used _/ --....,,__
                                                                                ********                   ********                               ********           ********               3.0           DEG. C.             CONTI       CALCTD REQUIREMENT EFFLUENT GROSS                                                                                                                                                                           DAILY MX                                 NUOUS FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT
1te Vice President SIGNATURE OF PRINC I PA L E XECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE 423 843-7001 17 06 07 I A R EA I NUMBER YEAR MO DAY CO DE Pa!'.le 1 of 1 PERMITTEE NAME/ADDRESS (I n clu de Fac ili t y Name/L ocation if Different)
                                                                                ********
Nam e _
                                                                                                      /908                        03              ********           ********             ********               ..      0     31 I 31     RCORDR 50050       1     0                                 PERMIT                   ******it*               Req. Mon.               MGD               ********             ********             ********             ****             CONTI       RCORDR REQU IREMENT EFF LUENT GROSS                                                                                       DAILY MAX                                                                                                                 NUOUS FLOW, IN CONDUIT OR THRU                             SAMPLE 1433                      ********                               ********             ********             ********                       0     31 I 31     CALCTD MEASUREMENT                                                                      03                                                                              03 TREATMENT PLANT 50050       1     0                                 PERMIT                Req. Mon.                     ********             MGD               ********             ********             ********           MGD               CONTI       CALCTD REQUIREMENT EFF LUENT GROSS VALUE                                                       MO AVG                                                                                                                                               NUOUS CHLORINE , TOTAL RESIDUAL                             SAMPLE MEASUREMENT
___ _ Addres.L_
                                                                                ********                   ********               ..              ********             0 .018               0.036               19        0     20 / 31       GRAB 50060       1     0                                 PERMIT REQUIREMENT
2000 ___________
                                                                                ********                   ********               ....             ********               0.1                 0.1               MG/L           FIVE PER CALCTD EFFLUENT GROSS VALUE                                                                                                                                                 MO AVG             DAILY MAX                                 WEEK TEMPERATURE - C, RATE OF CHANGE SAMPLE MEASUREMENT
_ ___ .J!.NTEROFFICE OPS-5 N-SQ.t:l.l
                                                                                ********                     0.2                 62
_______ _ NAT I ONAL POLLUTANT DISCHARGE ELI M INA TIO N SYSTEM (N POES) D I SCHARGE MONITORING REPORT (DMR) TN0026450 103 G MAJOR (SUBR 0 1) F -FINAL Form App r o ved. OMB No. 2040-000 4 _ _ _ _&sect;ODDY -DAISY .._lN_]7381_
                                                                                                                                                  ********           ********                                   ..      0     31 / 31     CALCTD 82234         1     0                                 PERMIT                  ********                     2.0               DEG               ********           ********             *******             ****             CONTI       CALCTD REQUIREMENT
_______ _ LOW VOL. WASTE TREATMENT POND Facl!l!Y _ _lYA -SEQUOYAH NUCLEAR PLANT _____ _ Locat i on_ _!jAMIL TO Ji.COUNTY.....
                                                                                                                                                                                  ) --------*-
_________
                                                      ''""'""",., ., ",_'" '"'" " '"' "'.,"'""--""'"'"&#xa5;.                                                         -----
_ EFFLUENT ATIN: M illi cent Gar l and NO DISCHARGE D ... NOTE: Read ins truct ion s before completinQ th i s form. PARAMETER IX QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UN ITS MIN I MUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ******** ******** 7.2 ******** 7.5 0 5 / 31 GRAB MEASUREMENT
C/HR EFFLUENT GROSS
.. 12 00400 1 0 PERMIT ******** ******** ** 6.0 ******** 9.0 SU ONCE/ GRAB EFFLUENT GROSS REQU I REMENT MINIMUM MAXIMUM WEEK SOLIDS , TOTAL SUSPENDED SAMPLE ******** ******** ******** 4.5 4.5 0 1 I 31 GRAB MEASUREMENT
                                                                                                    *- DAILY MX                                            _,/"                                                                  NUOUS
.. 19 00530 1 0 PERMIT ******** ******** .. ******** 30.0 100.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQU I REMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE ******** ******** ******** <5.0 <5.0 0 1 I 31 GRAB MEASUREMENT  
                                                                                                                                                        /
.. 19 00556 1 0 PERMIT ******** ******** .. ******** 15.0 20.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH FLOW , IN CONDUIT OR THRU SAMPLE 1.679 1.757 ******** ******** ******** 0 4131 INST AN 03 ** TREATMENT PLANT MEASUREMENT 50050 1 0 PERM I T Req. Mon. Req. Mon MGD ******** ******** ********* ** ONCE/ INST AN EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX WEEK SAMPLE MEASUREMENT PERMIT REQU I REMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERM I T REQUIREMENT --*--NAME/TITLE PRINCIPAL EXECUTIVE OFF ICER "'"''' &deg;' '"'" '"'' "
NAME/TITLE PRINCIPAL EXECUTIVE OF FICER direction or supervision in accordance with a system designed lo assure thal qualified
'"  
_ /                                      TELEPHONE                    DATE
-* p ,.,"."' TELEPHONE DA T E d irection or supervis i on in accordance w i th a system des i gned to assure that qual i f ied Anthony L. W il liams personne l p roperly gather and evalua t e the i nformat ion submi ll ed. Based on my i nqu i of th e person or perso ns who manage the system , or those persons directly responsible fo ather i ng th e i nformation , the information subm ille d i s , to the best of my knowledge and be li , true , 423 843-7001 17 06 07 Site V ic e President accurate , and comp le te. I am aware that there are s i gn i ficant penalt i es for submilli fa l se SIGNATURE OF PRINCIPAL EXECUTIVE I information, i n clu d i ng the poss i bili t y of fine and i mprisonment fo r knowing violations. O FF ICER OR AUTHORIZED AGENT ARE A I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY V I OLATIONS (Referen ce all attachments here) EPA Form 3320-1 (REV 3/99)
                                                                                                                                                              ~ice Anthony L. W illi ams                 personnel properly galher and evaluate the information submilled. Based on my inquiry o the person or persons who manage the system, or those persons directly responsible for gal ering P re:de nt lhe information, the informalion submilled is , to the best of my knowledge and belief, true,                                                       423         843-7001      17     06       07 S ite V ice President                 accurate, and complele. I am aware that there are significant penalties for submitling false          SIGNATURE OF PRINCIPAL EXECUTIVE                     I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.                 OFFIC ER OR AUTHO RIZED AGENT              AREA CO DE I   NUMBER       YE AR     MO     DAY COMMENTS AND EXPLANATIO N OF ANY VIO LATIONS (Reference all attachments here)
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if D ifferent)
No c losed m o de operation . T he following injecti ons o ccu rred : Spectrus CT 1300 (max calc. was 0.039 mg/ml, limit 0.05 mg/L) , Spec t rus BO 1500 (max ca lc. was 0.046 m gfml, limit - 2 .0 mg/ml) .
Name _
EPA Form 3320-1 (REV 3199)               Previous editions may be used                                                                                                                                                       Pf!OP. 1 of 1
___ _ Addres_L 20QQ... ___________
 
_ ___ .J.[NTEROFFICE OPS-5N-S(llil
PERMITTEE NAME/ADDRESS         (Include Facilitv Name/Location if Different)                           NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ                  MAJOR                                  Form Approved .
_______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (N PDES} DISCHARGE MONITORING REPORT (DMR} TN0026450 110 G MAJOR (SUBR 01) F -FINAL Form Approved. OMB No. 2040-0004 _ _ _ _.&sect;.ODDY -DAISY JN_1738L_
Name_~VA~EQUOYA~UCLEA~LANT _ _ _ _                                                                                  DISCHARGE MONITORING REPORT (DMR)                                                                   OMB No. 2040-0004 (SUBR 01)
_______ _ Fac.illJY_ .JYA -SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l_
Addres_L _&#xa3;. ~BOX..&#xa3;900 - - - - - - - - - - -                          -
_!!AMIL TOJi..COUNTy_
_ _ _ .J!NTEROFFICE OPS-5N-SQNl _ _ _ _ _ _ _                            _                                          TN0026450                                      101 T          F - FINAL
_________
_ _ _ _&sect;ODDY - DAISYJ.N_..]7381._ _ _ _ _ _ _ _                          _                                      PERMIT NUMBER                        DISCHARGE NUMBER            BIOMONITORING FOR OUTFALL 101 Fac_ill!y_ ..JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _                      _
_ RECYCLED COOLING WATER EFFLUENT ATIN: Millicent Garland ... NO DISCHARGE I xx I ... NOTE: Read instructions before completini:i this form. PARAMETER x QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** .. CENTIGRADE MEASUREMENT 00010 1 0 PERMIT ******** ******** ** ******** ******** REPORT EFFLUENT GROSS VALUE REQUIREMENT DAILY MX TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** .. CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ******** ******** ** ******** ******** 30.5 INSTREAM MONITORING REQUIREMENT DAILY MX TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** .. UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT ******** ******** .. ******** ******** 5 EFFLUENT GROSS VALUE REQUIREMENT DAILYMX FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** ******** 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** EFFLUENT GROSS VALUE REQUIREMENT DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** .. MEASUREMENT 50060 1 0 PERMIT ******** ******** .. ******** 0.1 0.1 EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILYMX TEMPERATURE  
LocatiO.!L _!!AMIL TOJi.COUNTY._ _ _ _ _ _ _ _ _ _                      _                                                                                                        EFFLUENT DAY 31 NO DISCHARGE            D ...
-C, RATE OF SAMPLE ******** ******** ******** ******** 04 CHANGE MEASUREMENT 82234 1 0 PERMIT ******** 2 DEGC ******** ******** ******** EFFLUENT GROSS VALUE REQUIREMENT DAILY MX SAMPLE MEASUREMENT PERMIT REQUIREMENT v----* --------/ --j----------
ATIN :Millicent Garland NOTE: Read instructions before completini:i th is form.
---NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 'C"'fy ""'" ""''" * ""''"" '"'  
                                              ><
'"" "'  
PARAMETER                                                                QUANTITY OR LOADING                                                  QUALITY OR CONCENTRATION                                NO. FREQUENCY SAMPLE EX          OF          TYPE ANALYSIS AVERAGE                    MAXIMUM              UNITS                MINIMUM            AVERAGE            MAXIMUM              UNITS IC25 STATRE 7DAY CHR                                SAMPLE                                              ********                                  >100 .0            ********           ********                               1/180      COMPOS MEASUREMENT
"""""" direction or supervision i n accordance with a syslem designed to assure that qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inq u i ry the person or persons who manage lhe sys tem, or those persons directly responsible for g thering the i nformation , the i nformation submitted is , to the best of my knowledge and bel ie f , Ir Site Vice Pres i dent accurate , and complete. I am aware that there are significant penalties for submitting fa lse information , includin g the poss i bility of fine and imprisonment for know i ng violat i ons. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Per i od EPA Form 3320-1 (REV 3/99) Previous editions may be used / ice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous .. ** CONTIN RCORDR uous 19 MG/L Five per CALCTD Week .. .. CONTIN CALCTD uous TELEPHONE DATE 423 843-7001 17 06 07 I AREA I NUMBER YEAR MO DAY CODE Paqe 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
                                                                              ********                                         **                                                                             23      0 CERIODAPHNIA TRP38        1    0                                  PERMIT REQUIREMENT
___ _ AddresL
                                                                              ********                  ********
___________
                                                                                                                                ....                 42.8               ********           ********           PERCENT              SEMI      COMPOS EFFLUENT GROSS                                                                                                                                MINIMUM                                                                          ANNUAL IC25 STATRE 7DAY CHR                                SAMPLE                                              ********                                 >100.0              ********                                                   1/180      COMPOS MEASUREMENT
_ ___ _J!NTEROFFICE OPS-5N-SQN) _______ _ _ _ _ _.&sect;.ODDY -DAISY JN_j7384_
                                                                              ********                                         **                                                       ********             23      0 PIMEPHALES TRP6C        1   0                                  PERMIT REQUIREMENT
_______ _ Faclfily_  
                                                                              ********                   ********
...IVA -_fil:QUOYAH NUCLEAR PLANT _____ _ Location_
                                                                                                                                ....                42.8              ********          ********          PERCENT              SEMI      COMPOS EFFLUENT GROSS                                                                                                                                MIMINUM                                                                          ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
_!:!AMIL TOJiCOUNTL
                                                                                                                                                        /   _). --------_. ~-~-----
________ _ ATIN: Millicent Garland NATI O NAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER 1 1 MAJOR (SUBR 01) F *FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I xx I ... Form Approved. OMB No. 20 4 0-000 4 From I : To Hf I &deg;tf NOTE: Read instructions before completinQ this form. PARAMETER I X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MIN I MUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** ** 23 CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERMIT ******** ******** **** 42.8 ******** **"'*****
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared un~                                      _/                                      TELEPHONE                    DATE direction or supervision in accordance with a system designed to assure that qualified                                                    --....,,__
PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** .. 23 PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMIT ******** ******** **** 42.8 ******** ******** PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT  
Anthony L. Williams                 personnel properly gather and evaluate the information submitted. Based on my inquiry the person or persons who manage the system, or those persons directly responsible for g haring 1te Vice President the information, the information submitted is . to the best of my knowledge and belief, tru~ .                                                        423    843-7001        17      06    07 Site Vice President                 accurate, and complete. I am aware that there are significant penalties for submitting false          SIGNATURE OF PRINC IPA L EXECUTIVE                    I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.                 OFFICER OR AUTHORIZED AGENT                  AREA CODE I  NUMBER        YEAR      MO    DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                    (Reference all attachments here)
../,......
Toxicity was sampled April 30 - May 5 , 2017 .
-/ NAMErrlTLE PRINCIPAL EXECUTIVE OFFICER /__ TELEPHONE DATE ', .. ""'" .. '"" '"" ""-'"' ., """'-" . .,. .. '&#xa5; d i rect i on or super vi s i on i n accordance with a s y stem d e s i gn e d lo assur e Iha! qua li f i ed Anthony L. Williams personne l prop e rly gath e r and evaluate !he inform a ti o n submilled. Based on my i nqu i ry f !he ------pe r son or p e rs o ns who man ag e !h e syst e m , or !hose p er s o n s d i r ec tly r e sp o n s i ble f o r g lh e r i n g Site V i ce President  
EPA Form 3320-1 (REV 3/99)             Previous editions may be used                                                                                                                                                       Pa!'.le 1 of 1
!h e in f o r mation , !h e i nformat i o n subm ill ed i s . lo !he bes! of m y k n owl ed ge a nd be lief , l r u ac c u r ate , a n d com ple te. I am a wa r e I ha! !he re a r e si g nifica n t pe n a lt i e s f o r s ub mi ll i n g f al s e i nform a t i on , i n cl ud i ng th e p o s si b ili ty of f i ne and i mp ri s on m e nt f o r k n ow i ng vio lat i on s. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous edit i on s may be use d Site Vice President SIGNATURE OF PRINCIPAL EXECU TI VE OFFICER OR AUTHORIZED AGENT 423 843-7001 17 06 07 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1 PERM I TTEE NAME/ADDRESS (I nc lu de Fac i l ity Name/Locat ion if D i ffe rent) Name _
 
___ _ Add r es_L 2000 ___________
PERMITTEE NAME/ADDRESS (Include Facility Name/L ocation if Different)                           NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES)                                                    Form Approved .
_ ___ _ilNTEROFF I CE OPS-5N-SQNl _______ _ _ _ _ __&sect;.ODD..Y_
MAJOR Name _ _!VA~EQUOYA~UCLEARPLANT _ _ _ _                                                                       DISCHARGE MONITORING REPORT              (DMR)                                                OMB No. 2040-0004 (SUBR 01)
* DA I SY
Addres.L_ ...&#xa3;. ~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _
_______ _ FacJilly _ _.IVA-_fil:QUOYAH NUCLEAR PLANT _____ _ Locat i o..Q_ .J:!AMIL TOli_COUNTL
_ _ _ .J!.NTEROFFICE OPS-5 N-SQ.t:l.l _ _ _ _ _ _ _ _                                                        TN0026450                                103 G        F - FINAL
_________
_ _ _ _&sect;ODDY - DAISY.._lN_]7381_ _ _ _ _ _ _ _ _                                                                                                                    LOW VOL. WASTE TREATMENT POND Facl!l!Y_ _lYA - SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _
_ ATTN: Millicent Gar l and NA TI ONAL POL L U T A N T D I SC HA RGE ELI M I N A T I ON SYST E M (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 PERMIT NUMBER 118 G DISCHARGE NUMBER DAY 31 MAJOR (SUBR 0 1) F-FINAL WASTEWATER
Location_ _!jAMIL TOJi.COUNTY..... _ _ _ _ _ _ _ _ _ _                                                                                                              EFFLUENT NO DISCHARGE         D ...
& STORM WATER EFFLUENT ... NO DISCHARGE I xx I *** Fo r m App ro ved. OMB N o. 20 4 0-000 4 NOTE: Read instructions before complet i nQ t h i s form. PARAMETER I X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUEN C Y SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS M I NIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMP L E ******** ******** ******** ******** MEASUREMENT
ATIN :M illicent Garland NOTE: Read instructions before completinQ th is form .
.. 19 00300 1 0 PERMIT ******** ******** **** 2 ******** ******** MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** ******** .. 19 MEASUREMENT 00530 1 0 PERMIT ******** ******** ***" ******** ******** 100 MG/L TWICE/ GRAB EFFLUENT GROSS REQU I REMENT DAILY MX WEEK SOLIDS , SETTLEABLE SAMPLE ******** ******** ******** ******** .. 25 MEASUREMENT 00545 1 0 PERMIT ******** ********  
IX PARAMETER                                                       QUANTITY OR LOADING                                                 QUALITY OR CONCENTRATION                           NO. FREQUENCY SAMPLE EX       OF             TYPE ANALYSIS AVERAGE                     MAXIMUM             UNITS            MINIMUM          AVERAGE           MAXIMUM           UNITS PH                                           SAMPLE MEASUREMENT
.... ******** ******** 1 ML/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** 03 .. TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** ******** ********
                                                                        ********                   ********               ..                7.2           ********             7.5             12        0       5 / 31         GRAB 00400       1     0                         PERMIT
* ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MO AVG DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT  
                                                                        ********                   ********               **               6.0           ********             9.0             SU               ONCE/           GRAB REQUIREMENT EFFLUENT GROSS                                                                                                                       MINIMUM                             MAXIMUM                                 WEEK SOLIDS, TOTAL SUSPENDED                     SAMPLE MEASUREMENT
...----. / --NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Ce rt i fy und e r p enal t y of law th a t th is d ocu m en t an d a ll a tta c hm ents w ere p re p a red un de r TE L EPHONE DATE d irecti on or su p ervi s i on in accor d a n ce w i th a system d e s ign ed to as s u re t h at q u al i fied Anthony L. W i lliams personnel prope r ly gat h er an d evalu a te the i n forma t ion submi tt ed. B ased on m y i n qui of t h e -perso n or perso ns w ho ma nage t h e sys t em , o r t h ose pe r so n s d i r ec t ly respons i ble f ot gathe r i n g t he informat i on , the i nformation subm i tted i s . lo the best of my knowledge a n d belie true , 4 2 3 843-7 00 1 17 06 07 Site V i ce Pres i dent accurate , and comple t e. I am aware that there are s i gnificant pena l t i es for subm i tt in l se i--s1 G NATURE OF PR I NC I PAL EXE CUT IV E I information , including t h e possibi l ity o f fine a n d impr i sonmen t fo r know i ng violations. -OFFICER O R AUTHORIZED AGENT AR E A I NUMBER Y E AR MO DAY TYPED OR PR I NTED CO DE C OMMENTS AND EXPLANAT I ON OF ANY VIOLATIONS (Refer e nce a ll a ttachments here) Dur i ng th i s r eport i ng pe ri od , there has been n o flow from t he Dredge Pond other than that resu lti ng from ra i nfa ll. No Discha rg e th i s Per i od EPA Form 3320-1 (REV 3/99) Previous editions may be used Paae 1 of 1 TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE  
                                                                        ********                   ********               ..              ********           4.5               4.5             19        0       1 I 31         GRAB 00530       1     0                         PERMIT REQUIREMENT
                                                                        ********                   ********
                                                                                                                          ..             ********           30.0             100.0           MG/L               ONCE/           GRAB
                                                                                                                          ..
EFFLUENT GROSS                                                                                                                                           MO AVG           DAILY MX                               MONTH OIL AND GREASE                               SAMPLE                   ********                   ********                               ********           <5.0               <5 .0                       0       1 I 31         GRAB MEASUREMENT                                                                                                                                           19 00556     1       0                         PERMIT REQUIREMENT
                                                                        ********                   ********
                                                                                                                          ..             ********           15.0               20.0           MG/L               ONCE/           GRAB EFFLUENT GROSS                                                                                                                                           MO AVG           DAILY MX                               MONTH FLOW, IN CONDUIT OR THRU                     SAMPLE                                               1.757                                ********         ********         ********                             4131           INSTAN MEASUREMENT 1.679                                          03                                                                       **       0 TREATMENT PLANT 50050     1       0                         PERMIT                Req. Mon.                   Req. Mon               MGD             ********         ********         *********           **               ONCE/         INSTAN REQUIREMENT EFFLUENT GROSS                                                       MO AVG                   DAILY MX                                                                                                           WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                                                                            -                                / ~q--                --*-
                                              '&deg;"'~ ~** "'"''' &deg;' '~ '"'" '"'' *oc~"'.,, '" .,,~m*** -* """)~
                                                                                                                                              ~.~. p,.,"."'
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER                                                                                                                                                        TELEPHONE                     DATE direction or supervision in accordance with a system designed to assure that qualified Anthony L. W illiams          personnel properly gather and evaluate the information submilled. Based on my inqui of the person or persons who manage the system, or those persons directly responsible fo athering the information, the information submilled is , to the best of my knowledge and beli , true,                                               423       843-7001       17       06       07 Site V ice President         accurate, and complete. I am aware that there are significant penalties for submilli false          SIGNATURE OF PRINCIPAL EXECUTIVE               I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.               OFFICER OR AUTHORIZED AGENT         AREA CODE I   NUMBER       YEAR       MO     DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
EPA Form 3320-1 (REV 3/99)
 
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different)                                 NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES}             MAJOR                                    Form Approved .
Name _ _!VA-SEQUOYA~UCLEA~LANT _ _ _ _                                                                            DISCHARGE MONITORING REPORT (DMR}                                                               OMB No. 2040-0004 (SUBR   01)
Addres_L ..f.. ~BOX 20QQ... _ _ _ _ _ _ _ _ _ _ _ _
_ _ _ .J.[NTEROFFICE OPS-5N-S(llil _ _ _ _ _ _ _ _                                                              TN0026450                                  110 G        F - FINAL
_ _ _ _.&sect;.ODDY - DAISYJN_1738L_ _ _ _ _ _ _ _ _                                                                                                                          RECYCLED COOLING WATER Fac.illJY_ .JYA - SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _
Locatio.!l_ _!!AMIL TOJi..COUNTy_ _ _ _ _ _ _ _ _ _ _                                                                                                                    EFFLUENT
                                                                                                                                                                              ... NO DISCHARGE           Ixx I ...
ATIN :Millicent Garland x
NOTE: Read instructions before completini:i this form .
PARAMETER                                                           QUANTITY OR LOADING                                                 QUALITY OR CONCENTRATION                             NO. FREQUENCY SAMPLE EX          OF          TYPE ANALYSIS AVERAGE                   MAXIMUM             UNITS             MINIMUM         AVERAGE             MAXIMUM           UNITS TEMPERATURE, WATER DEG.
CENTIGRADE SAMPLE MEASUREMENT
                                                                            ********                 ********               ..              ********         ********                               04 00010         1   0                             PERMIT
                                                                            ********                 ********               **             ********         ********         REPORT             DEGC              CONTIN          CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX                               uous TEMPERATURE, WATER DEG.
CENTIGRADE SAMPLE MEASUREMENT
                                                                            ********                 ********               ..              ********         ********                               04 00010       z     0                             PERMIT
                                                                            ********                   ********               **             ********         ********             30.5           DEGC              CONTIN          CALCTD REQUIREMENT INSTREAM MONITORING                                                                                                                                                             DAILY MX                               uous TEMP. DIFF. BETWEEN SAMP.           &           SAMPLE                   ********                   ********               ..              ********         ********                               04
                                                                                                                              ..
UPSTRM DEG.C                                 MEASUREMENT 00016       1     0                             PERMIT
                                                                            ********                   ********                               ********         ********               5             DEGC              CONTIN          CALCTD REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                                                             DAILYMX                                 uous FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT
                                                                            ********                                         03              ********         ********           ********             ..
50050       1     0                             PERMIT
                                                                            ********               Req. Mon.               MGD               ********         ********           ********             **              CONTIN        RCORDR REQUIREMENT uous
                                                                                                                              ..
EFFLUENT GROSS VALUE                                                                               DAILY MX CHLORINE, TOTAL RESIDUAL                       SAMPLE                     ********                   ********                               ********
MEASUREMENT                                                                                                                                               19 50060       1     0                             PERMIT REQUIREMENT
                                                                            ********                 ********               ..             ********             0.1               0.1             MG/L              Five per        CALCTD
                                                                                                                                                                                                        ..
EFFLUENT GROSS VALUE                                                                                                                                         MO AVG             DAILYMX                                 Week TEMPERATURE - C, RATE OF                         SAMPLE                     ********                                                         ********         ********           ********
MEASUREMENT                                                                    04 CHANGE 82234         1     0                             PERMIT REQUIREMENT
                                                                            ********                       2             DEGC               ********         ********           ********             ..            CONTIN          CALCTD EFFLUENT GROSS VALUE                                                                               DAILY MX                                                                                                             uous SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                                                                  - -*    ------
                                                                                                                                                    /
v--
                                                                                                                                                                  -- j---------- ---     --
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER
                                                  'C"'fy ""'"
direction      ""''"
* in""''""
or supervision                *~m'"' '"" "' "'""'~"*- """""" "(~
                                                                                      '"' with a syslem designed to assure that qualified accordance                                                                    /                                      TELEPHONE                      DATE Anthony L. Williams             personnel properly gather and evaluate the information submitted. Based on my inquiry the person or persons who manage lhe system, or those persons directly responsible for g thering the information, the information submitted is , to the best of my knowledge and belief, Ir         ~e            ice President 423        843-7001        17      06      07 Site Vice President              accurate, and complete. I am aware that there are significant penalties for submitting false        SIGNATURE OF PRINCIPAL EXECUTIVE                I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.               OFFICER OR AUTHORIZED AGENT            AREA CODE I  NUMBER        YEAR        MO    DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                 (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3/99)           Previous editions may be used                                                                                                                                                 Paqe    1 of 1
 
PERMITTEE NAME/ADDRESS            (Include Facility Name/Location if Different)                          NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES)                                                            Form Approved .
MAJOR Name_~~-SEQUOYA~UCLEARPLANT _                                    _ _ _                                                DISCHARGE MONITORING REPORT                    (OMR)                                                OMB No. 2040-0004 (SUBR 01 )
AddresL __E. ~BOX.1QOO _ _ _ _ _ _ _ _ _ _ _ _
_ _ _ _J!NTEROFFICE OPS-5N-SQN) _ _ _ _ _ _ _ _                                                                      TN0026450                                      110 T          F *FINAL
_ _ _ _.&sect;.ODDY - DAISYJN_j7384_ _ _ _ _ _ _ _ _                                                                                                                                       RECYCLED COOLING WATER PERMIT NUMBER                      DISCHARGE NUMBER Faclfily_ ...IVA -_fil:QUOYAH NUCLEAR PLANT_ _ _ _ _ _
r~RING p~*~t Location_ _!:!AMILTOJiCOUNTL _ _ _ _ _ _ _ _ _                                                                                                                                      EFFLUENT ATIN :Millicent Garland                                                                                 From 1     v~~* I~~ : ~~=                  To  Hf ~~ I&deg;tf                 1 NO DISCHARGE          Ixx I ...
NOTE: Read instructions before completinQ this form .
IX PARAMETER                                                                 QUANTITY OR LOADING                                                     QUALITY OR CONCENTRATION                               NO. FREQUENCY       SAMPLE EX       OF           TYPE ANALYSIS AVERAGE                     MAXIMUM               UNITS             MINIMUM                AVERAGE             MAXIMUM         UNITS IC25 STATRE 7DAY CHR                                   SAMPLE                   ********                   ********                                                       ********
                                                                                                                                    **                                                         ********           23 CERIODAPHNIA                                     MEASUREMENT TRP3B       1     0     0                             PERMIT
                                                                                ********                   ********               ****             42.8                 ********           **"'*****     PERCENT               SEMI       COMPOS REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                           MINIMUM                                                                         ANNUAL IC25 STATRE 7DAY CHR PIMEPHALES SAMPLE MEASUREMENT
                                                                                ********                   ********               ..                                    ********           ********           23 TRP6C       1     0     0                             PERMIT
                                                                                ********                   ********               ****             42.8                 ********           ********       PERCENT               SEMI       COMPOS REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                           MINIMUM                                                                         ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                        ',. " '". '" '" ""-'"'.,"""'-" ..,. .'&#xa5;
                                                                                                                                                            ../,......
                                                                                                                            -                                               ~*--~--
                                                                                                                                                        /
NAMErrlTLE PRINCIPAL EXECUTIVE OFFICER
                                                            ~            ~., . *~                                            ~"                                      /__                                    TELEPHONE                    DATE Anthony    L. Williams Site V ice President direction or supervision in accordance with a system designed lo assure Iha! qualified personnel properly gather and evaluate !he information submilled. Based on my inquiry f !he person or persons who manage !he system, or !hose persons directly responsible for g lhering
                                                      !he information, !he information submilled is . lo !he bes! of my knowledge and belief, lru Site Vice President
                                                                                                                                                                                                  ------  423        843-7001        17      06      07 accurate, and complete. I am aware Iha! !here are significant penalties for submilling false        SIGNATURE OF PRINCIPAL EXECUTIVE                      I TYPED OR PRINTED information, including the possibility of fine and impri sonment for knowing violations.              OFFICER OR AUTHORIZED AGENT                AREA CODE I    NUMBER        YEAR      MO    DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                      (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3/99)               Previous editions may be used                                                                                                                                                        Page 1 of 1
 
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)                                  NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)             MAJOR                                  Form Approved .
Name _ _!VA-SEQUOYA~UCLEA~LANT _ _ _ _                                                                              DISCHARGE MONITORING REPORT (DMR)                                                              OMB No. 2040-0004 (SUBR  01)
Addres_L _f. ~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _
_ _ _ _ilNTEROFF ICE OPS-5N-SQNl _ _ _ _ _ _ _ _                                                                  TN0026450                                  118 G        F - FINAL
_ _ _ __&sect;.ODD..Y_* DAISYJN~ 7 3Bi_ _ _ _ _ _ _ _ _                                                             PERMIT NUMBER                    DISCHARGE NUMBER          WASTEWATER      & STORM WATER FacJilly_ _.IVA-_fil:QUOYAH NUCLEAR PLANT_ _ _ _ _ _
Locatio..Q_ .J:!AMILTOli_COUNTL _ _ _ _ _ _ _ _ _ _                                                                                                                        EFFLUENT DAY
                                                                                                                                                                              ... NO DISCHARGE            Ixx I ***
ATTN :Millicent Garland                                                                                                                                              31 NOTE: Read instructions before completinQ this form .
NO. FREQUEN CY SAMPLE IX PARAMETER                                                             QUANTITY OR LOADING                                                 QUALITY OR CONCENTRATION EX       OF           TYPE ANALYSIS AVERAGE                     MAXIMUM             UNITS           MINIMUM            AVERAGE           MAXIMUM           UNITS OXYGEN, DISSOLVED                 (DO)             SAMPLE MEASUREMENT
                                                                              ********                   ********               ..                                ********         ********           19 00300         1     0                               PERMIT
                                                                              ********                   ********             ****               2               ********         ********           MG/L             TWICE/         GRAB REQUIREMENT EFFLUENT GROSS                                                                                                                             MINIMUM                                                                       WEEK SOLIDS, TOTAL SUSPENDED                           SAMPLE MEASUREMENT
                                                                              ********                   ********               ..            ********           ********                             19 00530         1     0                               PERMIT
                                                                              ********                   ********             ***"           ********           ********           100             MG/L             TWICE/         GRAB REQUIREMENT EFFLUENT GROSS                                                                                                                                                                   DAILY MX                               WEEK SOLIDS, SETTLEABLE                               SAMPLE MEASUREMENT
                                                                              ********                   ********               ..            ********           ********                             25 00545         1     0                             PERMIT REQUIREMENT
                                                                              ********                   ********
                                                                                                                              ....           ********           ********             1             ML/L             ONCE/           GRAB EFFLUENT GROSS                                                                                                                                                                   DAILYMX                               MONTH FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT                                                                    03            ********           ********         ********           ..
50050         1     0                             PERMIT               Req. Mon.                 Req. Mon.               MGD             ********           ********         ********
* ONCE/         ESTIMA REQUIREMENT EFFLUENT GROSS                                                             MO AVG                   DAILYMX                                                                                                             BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                                                                                                                        ...-- --   . --~            --
                                                                                                                                                  /
                                                                                                                                                      ~P<e,;deot NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under                                                                    TE LEPHONE                  DATE direction or supervision in accordance with a system designed to assure that qualified Anthony L. W illiams              personnel properly gather and evaluate the information submitted. Based on my inqui of the person or persons who manage the system, or those persons directly responsible fot gathering                                              -
the information, the information submitted is . lo the best of my knowledge and belie true,                                                 423        843-7001      17       06       07 Site V ice President              accurate, and complete. I am aware that there are significant penalties for submittin lse                                                          I
                                                                                                                                            -      i--s1GNATURE OF PRINC IPAL EXECUTIVE TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.               OFFICER OR AUTHORIZED AGENT         AREA CODE I   NUMBER       YEAR      MO     DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During th is reporting pe riod , there has been no flow from t he Dredge Pond other than that resu lti ng from ra infall. No Discharge th is Period EPA Form 3320-1 (REV 3/99)             Previous editions may be used                                                                                                                                               Paae 1 of 1
 
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE  


==SUMMARY==
==SUMMARY==
Report Date: May 24, 2017 1. Facility I Discharger
 
: Sequoyah Nuclear Plant I TVA 2. County I State: Hamilton I Tennessee
Report Date: May 24, 2017
: 1. Facility I Discharger: Sequoyah Nuclear Plant I TVA
: 2. County I State: Hamilton I Tennessee
: 3. NPDES Permit#: TN0026450
: 3. NPDES Permit#: TN0026450
: 4. Type of Facility: Nuclear-Fueled Electric Generating Plant 5. Design Flow (MGD): 1,579 6. Receiving Stream: Tennessee River CTRM 483.6) 7. lQlO: 3,491 8. Outfall Tested: 101 9. Dates Sampled: April 30 -May 05, 2017 10. Average Flow on Days Sampled (MGD): 1834. 1834. 1817 11. Pertinent Site Conditions:
: 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
Production I operation data will be provided upon request. 12. Test Dates: May 02 -09, 2017 13. Test Type: Short-term Chronic Definitive
: 5. Design Flow (MGD): 1,579
: 6. Receiving Stream: Tennessee River CTRM 483 .6)
: 7. lQlO : 3,491
: 8. Outfall Tested: 101
: 9. Dates Sampled: April 30 - May 05, 2017
: 10. Average Flow on Days Sampled (MGD): 1834. 1834. 1817 11 . Pertinent Site Conditions: Production I operation data will be provided upon request.
: 12. Test Dates: May 02 - 09, 2017
: 13. Test Type: Short-term Chronic Definitive
: 14. Test Species: Fathead Minnows (Pimephales promelas)
: 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia) 15. Concentrations Tested(%):
Daphnids (Ceriodaphnia dubia)
Pimephales promelas:
: 15. Concentrations Tested(%):
UV treated Outfall 101: 10.7. 21.4, 42.8. 85.6. 100 UV treated Intake: 1 00 Ceriodaphnia dubia: Non-treated Outfall 101: 10.7. 21.4. 42.8. 85.6. 100 Non-treated Intake: I 00 16. Permit Limit Endpoint(%):
Pimephales promelas: UV treated Outfall 101: 10.7. 21.4, 42.8. 85.6. 100 UV treated Intake: 100 Ceriodaphnia dubia: Non-treated Outfall 101: 10.7. 21.4. 42.8. 85.6. 100 Non-treated Intake: I 00
Outfall ] 01: IC2.2. = 42.8% 17. Test Results: Outfall 101: Pimephales promelas:
: 16. Permit Limit Endpoint(%): Outfall ] 01 : IC2.2. = 42.8%
IC25. > 100% Ceriodaphnia dubia: lC.25. > 100% Page 1of94
: 17. Test Results: Outfall 101 : Pimephales promelas: IC25. > 100%
: 18. Facility Contact: Millicent Garland Phone#: (423) 843-6714 19. Consulting I Testing Lab: Environmental Testing Solutions, Inc. 20. Lab Contact: Jim Sumner Phone#: (828) 350-9364 21. TVA Contact: Donald W. Snodgrass Phone#: (256) 386-2787 22. Notes: Exposures to samples collected April 30 -May 05, 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids.
Ceriodaphnia dubia: lC.25. > 100%
The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Page 2of94 METHODS  
Page 1of94
: 18. Facility Contact: Millicent Garland         Phone#: (423) 843-6714
: 19. Consulting I Testing Lab: Environmental Testing Solutions, Inc.
: 20. Lab Contact: Jim Sumner                     Phone#: (828) 350-9364
: 21. TVA Contact: Donald W. Snodgrass           Phone#: (256) 386-2787
: 22. Notes: Exposures to samples collected April 30 - May 05, 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Page 2of94
 
METHODS  


==SUMMARY==
==SUMMARY==
Samples: 1. Sampling Point: Outfall l 01, Intake 2. Sample Type: Composite
 
Samples:
: 1. Sampling Point: Outfall l 01, Intake
: 2. Sample Type: Composite
: 3. Sample Information:
: 3. Sample Information:
Date Date Date (MM-DD-YY) (MM-DD-YY)
Date                     Date                                           Date (MM-DD-YY)               (MM-DD-YY)             Arrival       Initial   (MM-DD-YY)
Arrival Initial (MM-DD-YY)
Sample           Time (ET)               Time (ET)             Temp.       TRC*         Time (ET)
Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (o C) (mg/L) Last Used Bv 101 04-30-17 0805 to 05-01-17 1332 4.l , 2.2 t <0.10 05-02-17 0920 05-01-17 0705 05-03-17 0846 Intake 04-30-17 0710 to 05-01-17 1332 1.7 <0.10 05-02-17 0920 05-01-17 0610 05-03-17 0846 101 05-02-17 0705 to 05-03-17 1235 2.1 , l.7 t <0.10 05-04-17 0850 05-03-17 0605 05-05-17 0852 Intake 05-02-17 0730 to 05-03-17 1235 2.2 <0.10 05-04-17 0850 05-03-17 0630 05-05-17 0852 05-04-17 0730 to 05-06-17 0930 101 05-05-17 1230 1.1 , l.3 t <0.10 05-07-17 0952 05-05-17 0630 05-08-17 0833 05-04-17 0745 to 05-06-17 0930 Intake 05-05-17 1230 0.9 <0.10 05-07-17 0952 . 05-05-17 0645 05-08-17 0833 *TRC = Total Residual Chlorine t samples were c o llected in two 2.5 gallon cub i tainers. Temperature was measured in each cubitainer upon arrival. 4. Sample Manipulation:
ID             Collected               Received             (oC)       (mg/L)       Last Used Bv 04-30-17 0805 to                                                         05-02-17 0920 101                                  05-01-17 1332         4.l , 2.2t    <0.10 05-01-17 0705                                                           05-03-17 0846 04-30-17 0710 to                                                         05-02-17 0920 Intake                                05-01-17 1332             1.7       <0.10 05-01-17 0610                                                           05-03 -17 0846 05-02-17 0705 to                                                         05-04-17 0850 101                                  05-03-17 1235         2.1 , l.7t    <0.10 05-03-17 0605                                                           05-05-17 0852 05-02-17 0730 to                                                         05-04-17 0850 Intake                                05-03-17 1235            2.2       <0.10 05-03-17 0630                                                           05-05-17 0852 05-06-17 0930 05-04-17 0730 to 101                                 05-05-17 1230         1.1 , l.3 t   <0.10     05-07-17 0952 05-05-17 0630 05-08-17 0833 05-06-17 0930 05-04-17 0745 to Intake                                 05-05-17 1230             0.9       <0.10     05-07-17 0952
Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- l .0&deg;C) in a warm water bath. Page 3of94 Aliquots of Outfall l 0 l and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
                . 05-05-17 0645 05-08-17 0833
Pimephales promelas Test Organisms:
  *TRC = Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
: 1. Source: In-house Cultures 2. Age: < 24-hours old Test Method Summary: l. Test Conditions:
: 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- l .0&deg;C) in a warm water bath.
Static, Renewal 2. Test Duration:
Aliquots of Outfall l 0 l and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
: 3. Control I Dilution Water: Moderately Hard Synthetic
Page 3of94
: 4. Number of Replicates
 
: 1 5. Organisms per Replicate:
Pimephales promelas                   Ceriodaphnia dubia Test Organisms:
lQ 6. Test Initiation: (Date/Time):
: 1. Source:                     In-house Cultures                In-house Cultures
05-02-17 0832 ET 7. Test Termination: (Date/Time):
: 2. Age:                         < 24-hours old                    < 24-hours old Test Method Summary:
05-09-17 0857 ET 8. Test Temperature:
: l. Test Conditions:             Static, Renewal                  Static, Renewal
Outfall 101: Mean= 24.7&deg;C (24.2 -25 .1&deg;C) 9. Physical I Chemical Ce riodaphnia dubia In-house Cultures < 24-hours old Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic l 05-02-17 0920 ET 05-09-17 0827 ET Mean = 24.9&deg;C (24.6 -25.2&deg;C) Measurements
: 2. Test Duration:                                                 Until at least 60% of control females have 3 broods
: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each I 00% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and exposure test solutions were analyzed daily for pH and dissolved oxygen. 10. Statistics:
: 3. Control I Dilution Water: Moderately Hard Synthetic            Moderately Hard Synthetic
Page 4of94 Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
: 4. Number of Replicates :     1
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration)
: 5. Organisms per Replicate: lQ                                    l
Conducted May 02 -09, 2017 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used -days) (% Effluent) 1 2 3 4 5 6 7 Control , 100 100 100 100 100 100 100 UV-treated 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Control , 100 100 100 100 100 100 98 Non-treated Test Solutions Mean Dry Weight (mg) (replicate number) (%Effluent) 1 2 3 4 Mean Control , 0.632 0.723 0.732 0.703 0.698 UV-treated 10.7% 0.700 0.704 0.701 0.677 0.696 21.4% 0.730 0.701 0.689 0.771 0.723 42.8% 0.687 0.642 0.662 0.684 0.669 85.6% 0.633 0.719 0.657 0.666 0.669 100.0% 0.644 0.649 0.627 0.637 0.639 Intake 0.650 0.605 0.596 0.688 0.635 Control , 0.632 0.783 0.630 0.669 0.679 Non-treated IC 2s Value: > 100% Calculated TU Estimates:  
: 6. Test Initiation: (Date/Time): 05-02-17 0832 ET                 05-02-17 0920 ET
< 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LC so: TUc = 100/ IC 2s Page 5of94 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a C eriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration)
: 7. Test Termination: (Date/Time): 05-09-17 0857 ET               05-09-17 0827 ET
Conducted May 02 -09, 2017 using effluent from Outfall 101. Percent Surviving Test (time interval used -days) Solutions 1 2 3 4 5 6 7 (%Effluent)
: 8. Test Temperature: Outfall 101:       Mean = 24.7&deg;C             Mean = 24.9&deg;C (24.2 - 25 .1&deg;C)         (24.6 - 25.2&deg;C)
Control 100 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 . JOO Test Solutions Reproduction
: 9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each I 00% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
(#young/female/7 days) Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 33 28 30 32 35 30 32 30 32 31.2 10.7% 34 36 31 34 33 31 32 35 31 31 32.8 21.4% 37 36 31 33 32 34 31 33 32 37 33.6 42.8% 35 36 37 32 33 33 35 34 36 33 34.4 85.6% 37 35 34 38 32 38 38 36 39 32 35.9 100.0% 38 36 37 36 37 39 39 35 38 33 36.8 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LC 5 o: TUc = 100/ IC 2s Page 6of94 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration)
: 10. Statistics:       Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
Conducted May 02 -09, 2017 using water from Intake Percent Surviving Test (time interval used -da y s) Solutions 1 2 3 4 5 6 7 (% Effiuent)
Page 4of94
Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction
 
(#young/female/7 days) (% Effiuent)
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 33 31 32 34 34 31 28 32 30 31 31.6 Intake 38 34 36 37 35 31 37 32 34 32 34.6 IC2 s Value: > I 00% Calculated T U Estimates: < 1.0 TUc* Permit L imit: N I A Permit Limit: N I A 95% Confidence Limits: U pper Limit: NA L ower Limit: NA *TUa = 100/LC 5 o: TUc = 100/ IC 2 5 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Spec i es Date Time Duration Toxicant Results (IC 2s) Pimephales promelas May 02 -09 , 2017 0820 7-days KCI 0.67 g/L Ceriodaphnia dubia May 02 -09 , 2017 0911 7-days NaCl 1.07 g/L Page 7 of94 PBYSICALJCHEMICAL  
: 1. Results of a    Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)              (Type I Duration)
Conducted May 02 - 09, 2017 using effluent from Outfall 101 .
Test                            Percent Surviving Solutions                      (time interval used - days)
(% Effluent)       1       2      3        4        5      6          7 Control ,
100    100    100      100      100    100        100 UV-treated 10.7%        100    100    100        100      100    100        100 21.4%        100    100    100      100      100    100        100 42.8%       100     100     100       100     100     100       100 85.6%       100     100     100       100     100     100        100 100.0%       100     100     100       100     100     100       100 Intake      100     100     100       100     100     100       98 Control ,
100     100     100       100       100     100       98 Non-treated Mean Dry Weight (mg)
Test Solutions (replicate number)
(%Effluent) 1           2             3           4       Mean Control ,
0.632       0.723         0.732     0.703       0.698 UV-treated 10.7%         0.700       0.704         0.701     0.677       0.696 21.4%         0.730       0.701         0.689     0.771       0.723 42.8%         0.687       0.642         0.662     0.684       0.669 85.6%         0.633       0.719         0.657     0.666       0.669 100.0%         0.644       0.649         0.627     0.637       0.639 Intake         0.650       0.605         0.596     0.688       0.635 Control, 0.632       0.783         0.630     0.669       0.679 Non-treated IC2s Value: > 100%                   Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 42.8%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
      *TUa = 100/LCso: TUc = 100/ IC2s Page 5of94
 
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
: 2. Results of a   Ceriodaphnia dubia   Chronic/ 7-day Toxicity Test.
(Genus species)             (Type I Duration)
Conducted May 02 - 09, 2017 using effluent from Outfall 101.
Percent Surviving Test                       (time interval used - days)
Solutions 1       2       3       4         5     6       7
(%Effluent)
Control       100     100     100     100       100   100     100 10.7%       100     100     100     100       100   100     100 21.4%         100     100     100     100       100   100     100 42.8%         100     100     100     100       100   100     100 85 .6%       100     100     100     100       100   100     100 100.0%       100     100     100     100       100   100 . JOO Reproduction (#young/female/7 days)
Test Solutions Data (replicate number)
(% Effluent) 1   2     3   4     5     6     7     8   9   10 Mean Control     30 33   28   30   32   35   30   32 30   32   31.2 10.7%       34 36   31   34   33   31   32   35 31   31   32.8 21.4%       37 36   31   33   32   34   31   33 32   37   33.6 42 .8%       35 36   37   32   33   33   35   34 36   33   34.4 85.6%       37 35   34   38   32   38   38   36 39   32   35.9 100.0%       38 36   37   36   37   39   39   35 38   33   36.8 IC25 Value: > 100%             Calculated TU Estimates : < 1.0 TUc*
Permit Limit: 42.8%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
    *TUa = 100/LC 5o: TUc = 100/ IC2s Page 6of94
 
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
: 2.       Results of a   Ceriodaphnia dubia     Chronic/ 7-day Toxicity Test.
(Genus species)               (Type I Duration)
Conducted May 02 - 09, 2017 using water from Intake Percent Surviving Test                         (time interval used - days)
Solutions 1       2         3       4         5       6       7
(% Effiuent)
Control       100     100       100     100       100     100     100 Intake       100     100       100     100       100     100     100 Reproduction (#young/female/7 days)
Test Solutions Data (replicate number)
(% Effiuent) 1   2     3     4     5     6     7     8   9   10   Mean Control     33 31   32   34   34   31   28     32   30   31   31.6 Intake       38 34   36   37   35   31   37     32   34   32   34.6 IC2s Value: > I 00%             Calculated TU Estimates: < 1.0 TUc*
Permit Limit: NI A Permit Limit: NI A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
        *TUa = 100/LC 5o: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species                            Date               Time Duration       Toxicant   Results (IC2s)
Pimephales promelas         May 02 - 09, 2017         0820     7-days     KCI       0.67 g/L Ceriodaphnia dubia         May 02 - 09, 2017         0911     7-days     NaCl       1.07 g/L Page 7 of94
 
~    PBYSICALJCHEMICAL  


==SUMMARY==
==SUMMARY==
CQ Water Chemistry Mean Values and Ranges fi>r UV-treated Pimephales promelas and Non-treated Ceriodaphnia dubia , Sequoyah Nuclear Plant (SQN), Efiluent Outfiill 10 l and Intake CD perfi>nned May 02-09 , 2017. ClO 0 .... Test Sample ID Temperature
 
("C) Dissolved Oxn:en (llll?fL)
CQ                 Water Chemistry Mean Values and Ranges fi>r UV-treated Pimephales promelas and Non-treated Ceriodaphnia dubia, Sequoyah Nuclear Plant (SQN), Efiluent Outfiill 10 l and Intake CD                                                                                               perfi>nned May 02-09, 2017.
Initial Final Initial Final Control, 24.7 24.5 7.8 7.0 Non-treated 24.6 -24.8 24.3 -24.8 7.7 -7.9 6.0 -7.8 Control, 24.7 24.4 8.0 7.1 UV-treated 24.6 -24.8 24.2 -24.7 7.9 -8.2 6.2 -7.7 10.7% 24.8 24.6 8.0 7.1 24.7 -24.9 24.4 -24.9 7.9 -8.1 6.2 -7.7 i: 21.4% 24.9 24.5 8.0 7.0 <>.. 24.7 -25.0 24.3 -24.7 7.9 -8.2 6.1 -7.7 "' 24.9 24.6 8.0 6.9 "' 42.8% --= 24.8 -25.0 24.2 -24.9 7.9 -8.1 6.0 -7.7 85.6% 25.0 24.6 8.0 6.9 Q., 24.9 -25.1 24.2 -24.9 7.9 -8.1 6.0 -7.7 100% 25.0 24.6 8.0 6.9 24.9 -25.1 24.2 -24.8 7.9 -8.1 5.9 -7.7 Intake 25.0 24.6 8.0 7.0 24.9 -25.l 24.4 -24.9 7.9 -8.1 5.9 -7.7 Control, 24.7 25.0 7.8 7.9 Non-treated 24.6 -24.8 24.9 -25.2 7.7 -7.9 7.8 -8.0 10.7% 24.8 25.0 7.8 7.9 24.7 -24.9 24.7 -25.2 7.8 -7.9 7.7 -8.0 .s 24.8 25.0 7.8 7.9 ..,, 21.4% .&sect; 24.7 -24.9 24.8 -25.2 7.8 -8.0 7.8 -8.0 .s 24.8 24.9 7.9 7.9 =: 42.8% --= .&sect;' 24.7 -24.9 24.8 -25.0 7.8 -8.0 7.8 -8.0 85.6% 24.9 24.9 7.9 8.0 24.7 -25.0 24.8 -25.1 7.8 -8.0 7.9 -8.1 100% 24.9 24.9 8.0 8.0 24.7 -25.0 24.8 -25.2 7.9 -8.0 7.9 -8.2 Intake 24.9 24.9 8.0 8.0 24.7 -25.0 24.8 -25.0 7.9 -8.1 7.8 -8.2 *Note: Total residual chlorine was perfonned on non-treated Outfall 101 and Intake samples. Overall temperature
ClO
("C) Pimephales prome/as Ceriodaphnia dubia Average 24.7 24.9 Minimum 24.2 24.6 Maximum 25.l 25.2 Initial Final 8.13 7.69 8.08 -8.21 7.49 -7.92 8.09 7.72 8.05 -8.19 7.55 -7.93 8.11 7.67 8.06 -8.18 7.49 -7.89 8.11 7.67 8.04 -8.17 7.48 -7.87 8.09 7.67 7.98 -8.18 7.49 -7.87 8.09 7.67 7.99 -8.19 7.49 -7.86 8.08 7.65 8.03 -8.19 7.48 -7.87 8.09 7.69 8.03 -8.18 7.48 -7.91 8.13 8.03 8.08 -8.21 7.97 -8.09 8.12 8.02 8.06 -8.18 7.97 -8.09 8.13 8.01 8.09 -8.19 7.97 -8.08 8.12 8.02 8.08 -8.19 7.97 -8.09 8.12 8.02 8.08 -8.19 7.97 -8.10 8.08 8.01 7.95 -8.19 7.92 -8.09 8.09 8.04 8.02 -8.18 7.98 -8.11 Conductance Alkalinity Hanlness *Total Residual (&#xb5;mhos/cm) (mg/L CaC03) (mg/L CaC03) Chlorine (mg/L) 322 62 85 -317 -328 61 -63 82 -320 62 87 -312 -326 61 -64 86 -307 ---295 -318 ------294 ---283 -305 ------259 ---250 -267 ------190 ---186 -193 ------165 64 65 <0.10 160 -169 59 -69 64 -66 <0.10 -<0.10 165 64 67 <0.10 158 -170 61 -67 64 -70 <0.10 -<0.10 322 62 85 -317 -328 61 -63 82 -308 ---298 -316 ------292 ---286 -300 ------258 ---248 -265 ------188 ---182 -196 ------164 63 65 <0.10 158 -171 59 -67 62 -66 <0.10 -< 0.10 166 64 66 <0.10 162 -177 59 -67 64 -68 <0.10 -<0.10 
....
0        Test           Sample ID       Temperature ("C)         Dissolved Oxn:en (llll?fL)                 pH~S.U.)          Conductance  Alkalinity  Hanlness          *Total Residual
~                                        Initial       Final         Initial           Final           Initial        Final    (&#xb5;mhos/cm) (mg/L CaC03) (mg/L CaC03)        Chlorine (mg/L)
Control,      24.7          24.5            7.8             7.0            8.13          7.69        322          62          85                      -
Non-treated 24.6 - 24.8 24.3  -  24.8 7.7     - 7.9    6.0  -   7.8   8.08 - 8.21    7.49 - 7.92 317  -  328  61  -  63  82 - 88      -                    -
Control,      24.7         24.4            8.0            7.1            8.09          7.72        320          62          87                        -
UV-treated 24.6 - 24.8 24.2 - 24.7 7.9         - 8.2    6.2   -   7.7   8.05 - 8.19    7.55 - 7.93 312  -  326  61  -  64  86 - 88      -                    -
24.8          24.6            8.0             7.1            8.11          7.67        307          -            -                      -
            ~              10.7%
24.7 - 24.9 24.4 - 24.9 7.9         - 8. 1 6.2    -   7.7   8.06 - 8.18    7.49 - 7.89 295 - 318      -        -  -      -    -                    -
              ~
i:          21.4%
24.9          24.5            8.0            7.0            8.11          7.67        294            -            -                      -
              <>..                    24.7 - 25.0 24.3  -   24.7 7.9     - 8.2    6. 1 - 7.7       8.04 - 8. 17  7.48 - 7.87 283  - 305  -        -  -      -    -                    -
            ~ "'                          24.9         24.6            8.0              6.9             8.09          7.67        259            -            -                      -
              "'
            --=            42.8%
24.8 - 25.0 24.2 - 24.9 7.9         - 8.1 6.0 - 7.7          7.98 - 8.18    7.49 - 7.87 250  -  267  -        -  -      -    -                    -
            -~
Q.,            85.6%
25.0          24.6            8.0             6.9            8.09          7.67        190          -            -                      -
24.9 - 25.1 24.2 - 24.9 7.9 - 8.1 6.0               -  7.7    7.99 - 8.19    7.49 - 7.86 186  -  193  -        -  -      -    -                    -
25 .0        24.6            8.0            6.9            8.08          7.65        165          64          65                    < 0. 10 100%
24.9 - 25.1 24.2 - 24.8 7.9        - 8. 1 5.9    -  7.7    8.03 - 8.19    7.48 - 7.87 160  -  169  59  -   69  64  - 66  < 0.10    -           < 0. 10 25.0         24.6            8.0             7.0            8.09          7.69        165          64          67                    < 0.10 Intake 24.9 - 25.l 24.4 - 24.9 7.9         -  8.1 5.9      -  7.7    8.03 - 8.18    7.48 - 7.91 158  - 170 61    -  67  64 - 70    < 0.10    -          < 0.10 Control,      24.7         25.0           7.8             7.9             8. 13          8.03        322          62          85                        -
Non-treated 24.6 - 24.8 24.9 - 25.2 7.7        - 7.9   7.8   -   8.0    8.08 - 8.21    7.97 - 8.09 317  -  328 61    -  63  82  - 88    -                    -
10.7%
24.8          25.0            7.8             7.9            8.12          8.02        308            -            -                      -
            .s 24.7 - 24.9 24.7 - 25.2 7.8        - 7.9    7.7   -  8.0    8.06 - 8.18   7.97 - 8.09 298  -  316  -        -  -      -    -                    -
            ..,,                          24.8         25.0            7.8              7.9            8.13          8.01        292            -            -                       -
            .&sect;            21.4%
            .s=:
24.7 - 24.9 24.8 - 25.2 7.8        -  8.0    7.8   -   8.0    8.09 - 8.19    7.97 - 8.08 286 - 300      -        -  -      -    -                    -
            --=            42.8%
24.8         24.9            7.9              7 .9            8.12           8.02       258            -            -                       -
            .&sect;'                      24.7 - 24.9 24.8 - 25.0 7.8        - 8.0    7.8 - 8.0        8.08 - 8.19   7.97 - 8.09 248  - 265  -       -  -      -    -                    -
            *~            85.6%
24.9          24.9            7.9              8.0            8.12          8.02         188          -            -                      -
            ~
24.7 - 25.0 24.8 - 25.1 7.8        -  8.0    7.9  -  8.1    8.08 - 8.19    7.97 - 8. 10 182 - 196    -       -   -     -     -                   -
24.9          24.9            8.0              8.0            8.08          8.01        164          63          65                    <0.10 100%
24.7 - 25.0 24.8 - 25.2 7.9        - 8.0    7.9  -   8.2    7.95 - 8.19    7.92 - 8.09 158  - 171 59    -   67  62  - 66  < 0.10    -         < 0. 10 24.9          24.9            8.0              8.0            8.09          8.04        166          64         66                    < 0 .10 Intake 24.7 - 25.0 24.8 - 25.0 7.9        - 8.1    7.8  -   8.2    8.02 - 8.18    7.98 - 8.11 162 - 177    59    -   67 64    - 68  < 0.10    -         < 0. 10
      *Note : Total residual chlorine was perfonned on non-treated Outfall 101 and Intake samples.
Overall temperature ("C)                        Average        Minimum        Maximum Pimephales prome/as            24.7          24.2            25.l Ceriodaphnia dubia            24.9          24.6            25.2


==SUMMARY==
==SUMMARY==
I CONCLUSIONS Exposures to samples collected April 30-May 05 , 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids.
I CONCLUSIONS Exposures to samples collected April 30- May 05 , 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
The resulting IC25 values , for both species , were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Page 9of94 Appendix A ADDITIONAL TOXICITY TEST INFORMATION  
Page 9of94
 
Appendix A ADDITIONAL TOXICITY TEST INFORMATION


==SUMMARY==
==SUMMARY==
OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing
OF METHODS
: 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
: 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL
: 1. Pimephales promelas Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction oftest organisms. UV treatment is used to control interference of fish pathogens. This treatment method was approved on November 23 , 2015 by the State of Tennessee in a letter from Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring.
: 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS I. Pimephales promelas None
: 2. Ceriodaphnia dubia None Page 10of94
 
PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable) .
: 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
: 3. Temperature was measured by SM 2550 B-2010.
: 4. Dissolved oxygen was measured by SM 4500-0 G-20 I 1.
: 5. The pH was measured by SM 4500-H+ B-2011.
: 6. Conductance was measured by SM 2510 B-2011.
: 7. Alkalinity was measured by SM 2320 B-2011.
: 8. Total hardness was measured by SM 2340 C-2011.
: 9. Total residual chlorine was measured by ORION 97-70-1977.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
: 1. Test Type: 7-day chronic tests with results expressed as IC2s values in g/L KCI or NaCl.
: 2. Standard Toxicant: Potassium Chloride (KC! crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
: 3. Dilution Water Used: Moderately hard synthetic water.
: 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 11of94
 
REFERENCES l . NPDES Permit No. TN0026450.
: 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-82l-R-02-013 (October 2002).
: 3. Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012.
: 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 12of94
 
Sequoyah Nuclear Plant Biomonitoring May 02 - 09, 2017 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 13of94
 
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 2017 Date      Sodium    Towerbrom  PCL-222    PCL-401  CL-363  Cuprostat-PF H-130M  Nalco H-150M Hypochlorite    mg/L      mg/L      mg/L    mg/L        mg/L      mg/L  73551  mg/L mg/L        TRC    Phosphate  Copolymer DMAD        Azole      Quat  mg/L  Quat TRC                                                                    EO/PO 02/06/2005      -        <0.0042    0.028      0.010      -        -          -      -
:
:
1tl <l-OOb A\l... S".-\P\tS lnL vd //y//l 5=!-17 SOt..l\(..
                                                                                                                                .. -                                                                    -* .-i..;.                                                                ~
tJS---0/-1? / o<e.*) *&#xa3;T (....-v /
                                                                                                                                                                                                                            -                                  ~
-tSI /3.3\.. I / Bl( 1-tr"J (' 1"\
100%
Er "---" Instructions
* AJknlinity                                                      -      .                                                                                                  -;;                                      ' r
: Clients should fill in all areas except those in the "Laboratory Use'* block. Biomonitoring samp l es are preserved by storing them al 6&deg;C and shipping them in ice. The hold time for each sample is 36 hou r s from the time of collection.
                                                                                                                                                                                                                                                                      -~
Therefore. please collecl and ship in such a way that the laboratory wil receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive un t il the following Monday.
                                                                                                                                                                                                                                                              - . -- - ..
Whole Effluent Samp l e Receipt Log Page 52 *s ample
                                                                                                                                                                                                                                                                            .
(mg CaCO;/Ll
                              *ll:irdncss                    -  - !
                                                                              -
                                                                                                              ""&deg;'
(.~
                                                                                                                                                          .
                                                                                                                                                                                    -
                                                                                                                                                                                                    ' ~:l
                                                                                                                                                                                                          .~              ,*
                                                                                                                                                                                                                                                    .
(mg CaCO;/L)
                                                                                  -
                                                                                            ~
                                                                                              . -                                                  - -                            -                          *~ .' ~          ,_
                                                                                                                                                                                                                                    -    ~
                                                                                                                                                                                                                                                  -'.- ' - -
                              *TR chlorin r (mg/L)
                              *Tempera ture (&deg;C)
                                                              ~* - .
                                                                '"\S. \
                                                                          -
                                                                                    "\!-\. s
                                                                                                    .      I f"l.I(')
                                                                                                              -u;. \
                                                                                                                                  .4}- ...
                                                                                                                                  -r-\*.S
                                                                                                                                                        --          L.S.O
                                                                                                                                                                                '
                                                                                                                                                                                                "'t.L\*1-
                                                                                                                                                                                                            '*
                                                                                                                                                                                                                                        ~
                                                                                                                                                                                                                                      \.~.    \                '1..-t.~
DH (S.U.)                          ~DS            -'J,,f'C(                  ~.'i::l"1-          r~.lJ2.                            0.0""}                .tlC\\                                  {:). la                '}.~0 DO (ml!/Ll
* fh*c. D
* fh*c. D
* dr J d), SB* spG 1 bro o d (.s 1n_.;lc brood 5p lu bc 1 wc-cn 1 wo d l)'i}, CO* c urv (offs p n n i:: co mrd o v er wn b a du ll dun n 12. L r:1 rufcr) Co 11 ce11trntio11
* drJd), SB
: % Mor1ali1 y: 01. Mean Offsp ri ng/F e male:
* spG1 brood (.s1n_.;lc brood 5plu bc1wc-cn 1wo dl)'i}, CO
% Reduct i on from Control-I: -
* curv O\'~ (offs pnni:: co mrd over wnb adull dunn12. Lr:1rufcr)
Page 39 of94 SOP ATI I -E x hibit AT! 1.2 , r evision 11-01-1 4 
Co11ce11trntio11:
\-----_,, c...1n.NN 1'U l rut lnqklv: l.:l*L a...:.. Pa g e 4 of 7 S p e ci es: Cerio dap!tuia tlu b itt C li e n t: TVA I Sc qu ovah N u c l ear Pla n t , Ou tfall 101 D ate: 05-02-17 CONC: 8 5.6% S ur v ival and R e produ c tion D ata l{en l ica t c n umber Da y I 2 3 4 5 6 7 8 9 10 I You n g pro du ced [) 0 CJ 0 0 (\ 0 l&deg;'I a 0 Ad ult mortalit y L L '-L \_ '--'---'-'--'-2 You n g p r o duc ed 0 () A {) (\ 0 (') 0 0 n Ad ult mor t a li ty \ '--L \_ '--\._ '-\_ '-.__ 3 You n g pr o du ced fl 0 0 0 0 0 a 0 0 0 Adult mortality L-'-'--\_ '-L L '-\..... \.. " Youn g pr o d uced '-5 -::. '&deg; '-\ l.. $ 'S \..\. A dult morta l ity '--'-'-\_ \_ \_ \....... '--'-'--5 Young pro d u c ed \ '"L \C..\ \\ \ '&deg;';:) l\ A dult morta l it y '--L \...... '--L \__. '---'-\....... \._ 6 Yo u ng prod uc ed 0 0 () 0 0 0 0 c 0 0 A du lt m o r talit y L '-'--L L. L-L-\..... \,_, L.. 7 Yo un g produced \ &. \ '\ l\ 1..0 \ .i. 1..o l\ To t a l y o un g pro du ce d !:>"""\
                                                                                                                              % Mor1ali1y:                                               01.
Fi na l A d u l t J\'io rt a lil)* '-'-'-.__ '--'-'-'--'---'--Not (: A d uh m oni h t y (L
Mean Offspri ng/Female:                                   3~.'t
* l wc, 0 *dead). SB =-sp l ll broocJ {sm.&#xa3;,lc b foOd s pi n b tl\ ... -ce n c w o CO s cu h d I r v O\'c r (o ffs o n ntt crned C\'ct wu a u l d u nnl! t ra n s er). Co 11 ce11t ra tion: % M ortali t y: n1 M co n OfTsp rin g/F ema l e:
                                                                                                                              % Reduction from Control- I:                             - ft>.~7.
% R e du c t i on from C ontrol-I : -'\S. \"l. C O N C: 100% S ur v i v al a 11d R e pr od u ctio11 D a t a Rco l icn t c nu m ber Da y I 2 3 4 5 6 7 8 9 10 I Y o un g pr od u ce d a ('> (\ n 0 () (') ('., (\ 0 A d u l t m ort ali t y '--L L '--L L L '-\_ L.. 2 Y oung pro du ced () 0 0 0 0 fl (\ 0 (""\ (1 Adult mor t a li t y *\...... '-'--\..._ '--'-\...._ '-L \..... 3 Yo u n g p rodu ced n 0 0 0 0 0 0 0 0 0 A dult morta lit y L. L-L L L L L L.. L L -I Y oung [Jro d uccd '-\. s .s l. s s Co '-\. Ad ul t mo r tn l it y '-'-'--"-L L \... \._... '--5 Yo un g prod u ced l'-\ ' ... l \ ,_ \'-\ \"b ''-\ \\ '""L J 1-Ad u lt m ortn li l)* .,, '-'-'-'-'-'-'-L.-'-6 Yo u ng pr o duc e d 0 a () 0 () 0 () 0 a ('"'\ Adu lt m or t a l i t y \__.. L L l--L \.. L.. L \...... 7 Young pr od u ced i..\ \""\ \i '2 10 L "\ l '\ 1...0 .., Total y o u a g proiluced 31 3'\ 3'\ Fina l A dult Mor t n l ity .__ '--\._.. \...__. \,_ '-'--'--'---\.._ NOi i*: Adult mon al ll y (L 11 lwc , D. SD .... split brood {t in!'J C bro o d spltr bc 1 wccn l WO d.iys:). co -Q n O\'Cf {offs'2 ri rut am c d o v t'.r with d urinc. l ransfcr l Co11c e 111ra t io11: % M ort al i t y: 61. M e an O ffsp rin g/Fema l e:
SOP ATI I - Exhibit AT ! 1.2, revision 11 14 Page 39 of94
% R ed uc tion fr om C o ntrol-I: -\\.'\ T. S OP A Tl I -Ex h i bit A T I 1.2, r ev i sio n 11-0 1-1 4 Page 40 of94 l Page 5 of? Species: Cerio dnpl111ia dubia Client; TVA I Sequoyah N ucle a r Plant, Ou tfall 10 1 Oa te: 05-02-17 CONTR O L-2 Survival and Reproduction Data Replicate numb er Day I 2 3 4 5 6 7 B 9 10 I Young produced (") 0 0 () () n 0 0 0 0 Adult mortnlit y '-'-'-'--L L L. '-'-'--2 Young produced 0 0 0 0 0 () a () 0 n Adult morta lit y '-'--'-"-\.._ '-'-'-'-3 Young produced 0 0 D (J (', 0 (') 0 c n Adult mortality L \..._ L-'-'-'-L-L_ L L... .j \'oung prod u ced w.. "-{ "-\ "' "'-\ "-:\ '-\ s "-\ Adull mortnlily
'-"-\._ '-L '--'-'-\,_ 5 Young pr.oduccd l '-\ "'\... \\ ''-\0 \0 \0 \\ Adult mortnlit y "-\._ "'-\..._ '--'--'-\.... '--._ G Young pro du ced 0 () 0 0 ('\ (j C) 0 () () Adult mort:tlit y \,_ \.._ L \..... '--\..._... '-L L 7 Young p r o duced \""\ \S \S 1'-1, \& I \o Total young produced :-,\ "!> '"l..
':!> "2. Finni Adult Mortality
\..... \.... '-\_ '-'-\..... '-\.... '-X for J'" l.lro o d s !'-')(_ /<--,c..... "><.. 7<--X-i\'01,*: Adt1lr D'IOn 3hl y (L *hv c. 0
* SO""' sphl brood (s m sle brood spli l between two d:r.y s). CO-any O\'c.r (offspnny earned o"c.r '"''h ;iduh dunns tnn.sfer). Co11ce11rrntio 11: % Mortality: I (")1. Mean Offspring/Fema l e: I CONC: 1 00% Intake Survival and Reprod11ctio11 Data Rcnlicatc n umber Dny I 2 3 4 5 G 7 8 9 JO I Young produced 0 0 0 0 0 0 0 D 0 l\ Al.lull mortnlity L '--L L '-'-'--'-'-L '.? \'oung prod u ced ("') n A n ("") (') 0 0 0 () Adult mortnlity L. '-L-\.._ '-L L '-\...... 3 You n g produced D () 0 0 c () 0 0 n G Adult mort:1li1y L L L L '-L L \.._ '-L 4 Young produced I.,. l.o s "' '--\ '4. s s Adult mortnlity
'-..__ '--'-'--....__ L '-\.._ I..._ 5 \'oung produced ' "?:> I\ ''-\. '"L "L.. \ 1... l '-l lL.. \ \ Adull morrnlity L '-'-\.._ \__... '--'-\_... \.... G Young pro du ced u () {) () 0 0 0 0 0 0 Adult mortality
'--L '-L L L L L-\.... L 7 Young pro du ced ''\ ,.., l\ \ C\ ''\ \ '\ \cs, 1 \o' no Tolnl you11g produced 1:,1.. Finni Mortality
\..... '--'-\.._ \_ L. '--L \,,_ .\'n1c: 1\duh mon.;1J ity (L z Jive. O
* dcd), SO* 1pl11 brood bm&#xa3;IC brood 1pht between two days). CO* C"1m' over O\'Cf wr l h oiduh dunne irlrW"cr)
Co11ce 11rrntio11
: % Mortality: 01. Mean OITspringlfemalc
:
% Redu ction from C o ntrol-2: -'\.$1. SOP AT I I -Exhibit ATI 1.2 , revision 1 1-01-14 Page 41of94 (Q CD "> 0 .... co Emiro nM t n t" I T0t.Ung 5olu1Jon'lo.
Inc. C o n l.-o l-1 ll uy I 2 J I 0 0 0 l 0 0 0 3 0 0 0 J J 6 J s 1 0 II 1 0 6 0 0 0 7 1 6 16 14 T o t ol JO JJ 28 I0.7% ll") I 2 J I 0 Q 0 ? 0 0 0 3 0 0 0 J 5 .1 s s 12 l<I 1 2 6 0 0 0 7 17 18 14 Tom i 3J J 6 31 2 1.4% U oy 1 2 J I 0 0 0 ? II 0 0 l 0 0 0 J s (1 4 5 I J 1:; 11 6 0 0 0 7 19 17 15 Tolul 37 36 JI 42.8% U ny I 2 J I 0 0 0 2 0 0 0 J 0 0 0 J J s 5 5 12 13 12 6 0 0 0 7 19 I ll 20 Tol*I JS J6 J7 Il l' 1licu l l' u u m lJcr J s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 s 5 5 J 12 11 13 1 0 0 0 0 0 1 3 1 5 17 16 JO 32 35 J O Ht111ic:11r u umlJ'-'r J s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 3 ,j .j II 1 3 l'I 12 0 0 0 0 18 1 7 1 3 1 6 JJ J3 JJ J?
numbtr J 5 6 7 0 0 0 0 u 0 0 0 0 0 0 _g 4 4 5 .j 1:; IO 1 3 12 0 0 0 I) 16 1 8 1 6 15 J J 32 JJ JI )licn l l' n 11 1nbcr J 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 4 4 5 12 1 4 10 1 4 0 0 0 0 1 5 1 5 I Q 16 J2 JJ JJ JS T V A I Seq uo ya h N ucl ea r Pl a nt , Outfall IO I May 02-09 , 2 0 1 7 Ve rifi c a ti on of Ce ri n t!apf111 ia Rep r odu c tion To ta l s 85.6% 8 9 10 To t nl D uy I ? J 0 0 0 0 I 0 0 0 0 0 0 0 ? 0 0 0 u 0 0 0 J 0 0 0 5 l s 46 4 6 s s 1 0 13 ti 112 ;; 13 12 14 0 0 0 0 G 0 0 u I? 1 4 16 ISJ 7 IS iK 15 J2 JO .)2 Jll 1'0101 J7 JS 34 100% s 9 1 0 Toll11 00) I 2 J 0 0 0 0 I 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 J u 0 0 4 5 5 *14 J 4 s 5 2 II 12 123 5 1 3 1.1 14 0 0 0 0 6 0 0 0 <) I) IJ 161 7 21 1 7 18 J; JI JI J 28 38 J6 37 Co ntro l-2 8 ? 1 0 Tomi Dn y I 2 J 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 *I .j 6 4(1 4 4 4 4 10 1 1 IJ 121 5 12 12 13 u 0 0 0 6 0 0 0 1 9 1 7 17 IC.9 7 1 7 15 IS J.) .ll 3 7 JJ 6 Tot n l 33 31 )2 !O il% I nt ak e 8 9 1 0 Toto I O*y I 2 .) 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 J 0 0 0 6 5 s 48 4 6 6 s 1 3 12 13 12 5 5 13 I I 14 0 0 0 0 , , 0 u u 1 5 19 IS 1 71 1 19 17 17 JJ J G JJ J44 Totnl J 8 J6 lt coll e1 11c num b et Toto! 4 5 6 7 K 9 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 4 6 s s 5 4 5 1 IJ I I IJ 13 13 14 II 1 2 7 0 0 o I o 0 0 0 0 1 9 1 7 19 I 20 IS lO 17 18 1 .)8 n JS I 38 J6 J9 J? JS9 n cullnk 110mbt!r T o mi 4 5 6 7 8 9 10 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 0 0 0 0 0 0 0 6 s 6 (> s 6 .1 n 12 1 4 1 3 l*I 1 1 I! 11 12 9 0 0 0 0 0 0 0 0 IS 18 20 19 19 w 17 1 87 36 37 39 39 JS 38 .l3 3611 ll cn licntc num)er T o 1ol J s 6 7 8 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 6 s 4 4 4 s 4 J 4 13 I I 12 10 10 1 0 I I 11*1 0 0 u 0 0 () 0 0 1 5 1 8 15 14 IS 1 5 I C. 15 8 JJ J 4 JI ?8 J2 JO JI 316 H t11 1i c t llc num b rr Totnl J 5 6 7 8 9 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 6 4 J 4 5 5 s so 12 12 12 14 1 2 IJ 1 1 124 u l1 0 u 0 0 0 0 19 1 9 15 1 9 15 lb 1 6 172 J7 JS JI 37 J? 34 32 34 6 ..f ....... -* -r Environm e ntal T e st i ng So lu tl on s, In c. Concc 11 l rn tion (%) C()ut ro l-I 111.7% 21.4% 85.6% 1 00% '0 111 ro l -2 I UO% l111 akc O utfoll 1 0 1: llu1111 e t1'
* i\I 'D v: 1 luc; l'M S D: Int *kc: llu 11 11 c 11's l'M**n: I 30 \4 37 35 37 3 33 38 2 3 33 18 3r , J I 36 31 36 3 7 35 34 36 37 31 32 J4 J6 2.1 1 3 6.8 1.663 5.3 TVA I Sequoyah Nucl e ar Plant , Outfall 101 May 02-09 , 2017 C hr o nic Whol e E fflu e nt Toxici ty T es t (EP A-821-R-0 2-013 , Method 100 2.0) Ce riodup/111i11 dubia Chronic Whol e E fflu e nt Toxicity Te s t E PA-821-R-02-013 , Method I002.0 Qu a li ty C ont ro l Ve rific a tion of Data E n t r y, C alcul a ti o n s, and S t*1ti s tical A nal y cs rr ojcr t n u mb e r: ___________ 12_J_J_2 R e ul icn t c num ber Sun r i\'n l ,\vcrngc r eprod u c t ion Cot ffiri tr H or f'rrcrnl rrtlnc 1 ion fr om (%} (orr, prini:lfcrnnle) v" r i:uio n ('Y*) 1 moh."4.I ron1rob (*!.) s 6 7 8 9 10 30 J2 35 30 32 30 32 JOO 3 1.2 GA No t ap pli ru b le 3J JJ 3 1 '2 J5 I 31 1 00 32.11 5.7 *5.1 33 32 34 J I 33 32 37 JOO 33.6 6.9 -7.7 32 JJ 33 35 J4 36 33 1 00 3H 4.8 -10.J 38 J_ 38 38 36 39 32 JOO 35.9 7.1 -I S.I 36 37 39 39 35 38 33 1 00 36.8 S.I -1 7.9 34 34 31 28 32 30 31 100 Jl.6 5.8 No t " lll l li c:i bl c 37 r JI '7 32 34 32 I OU JM 7.0 -9.S MSD = Minimum S i g n ilica nt Diflere n cc P M D"' Perce nt Minimum ig n ificant D ifference PM Dis 1.1 measu r e o ft est precision The PMSD 15 thc nnmmu111 percent d 1 1Tc rc ncc be t wee n lh c co n trol and lrcmment th at con be d ec l ared stu11st1cn ll s 1 g nilicant in a whok c lllucnt to x ic1t y test. Lower l'MSD bound dc tc n n111cd by USEP/\ ( 10 11' pcrccntlk)
= 13% Upper PM D bou nd dclcnnin c d by SE I'/\ (90 11'
= 47% L O\ er und upper l'MSI) bo und s were determined fr om the I 0 th nnd 90th pcrccn ll lc. rcsp..'C t1 vcly. or PMSD da t u from E P/\'s W ET !ntcrl a boruto ry Variabi l ity tud y(USEP/\.
2001a. USEI'/\. 2001b) llSEPA 2 I ll. 2rHll I , Fi 11:1I Report l11 1crl11bom t m y V:1rinb1hl r Study r E P A ShC1n-tcrn1 Chro n i c mid A cu te Whok Efll ucn l Tox i cit y Method s. V tilumcs I and E l'A-821-13-01-004 nm J 05 LI , Enviro nm c mul P rot e ct i on Agenc y, incmnati. M. 


S1a11 Date. 51212017 TV A I Sequoyah Nuclear Plant, Outfall 101 May 02-09, 2017 Statis tical Analy ses Ceriod:.pnnia Survival and Reproduction Test-Roproduction Test ID: C O FRCR Sarnple ID: TVA I SQN Ou L rau 101 End Da l e: 519/2017 Lab 10. ETS-Envi r. Tesling Sol Sample lype* OMR-Oischarge Monitoring Repon Sa mp le Date May 2017 Pro ocol. Fll\iCHR-EPA
      \ - -- - -
-821-R-02-013 Test Spec i es: CD-Cenoaaphnia dub ia Comments: Non-L
_,, c...1n.NN1'Ul rutlnqklv:l.:l* La...:..
Page 4 of 7 Species: Ceriodap!tuia tlubitt C lien t: TVA I Scqu ovah Nuclear Pla nt, Ou tfall 101                                                                                                                    Date: 05-02-17 CONC:          85.6%                                                                          S urvival and R eproduction Data l{en lica tc number Da y                                                      I            2              3              4            5            6              7            8                9                10 I              Young produ ced                      [)            0              CJ                0            0            (\            0              l&deg;'I          a              0 Ad ult mortality                        L          L              '-            L            \_
                                                                                                                                        '--            '---            '-                '--            '-
2              You ng pr oduced                      0              ()          A              {)          (\            0              (')              0            0                  n Ad ult mortali ty                      \            '--          L                  \_
                                                                                                                            '--            \._
                                                                                                                                                        '-            \_
                                                                                                                                                                                  '-                .__
3            Young produ ced                        fl        0              0                0            0              0            a              0              0              0 Adult mortality                        L-          '-          '--        \_
                                                                                                                    '-                L            L              '-              \.....            \..
Young produced
                                                                  '-        5              -::.                          '-\          l..            $            'S              ~              \..\.
          "              Adult morta lity
                                                                '--        '-          '-          \_  '&deg;              \_            \_            \.......    '--          '-              '--
5              Young produced                        \~          \ '"L          \C..\      \~              \\            \~            \~              \ '&deg;';:)    \~                  l\
Adult morta lity
                                                                '--            L            \......        '--          L              \__.
                                                                                                                                                        '---          '-                \.......        \._
6              Young prod uced                        0          0            ()            0                0              0            0              c                0                0 Adu lt mortality                        L        '-              '--            L              L.              L-          L-            \.....            \,_,        L..
7              Yo ung produced                    \ &.          \~            \~              \ '\          l\            \~            1..0          \ .i.          1..o                l\
Total young produ ced                                  !:>"""\      ~~              ~--\            ~~            ~1...          ~~          ~i              ~lo            ~'\              ~1..:..
Fi na l Ad ult J\'iortalil)*                                '-          '-          '-
                                                                                                        .__              '--          '-            '-              '--            '---            '--
Not( : Aduh moni hty (L
* lwc, 0
* dead). SB =- splll broocJ {sm.&#xa3;,lc bfoOd spin btl \...-cen cwo d ~ys ), CO s curv O\'c r (offs onntt crned C\'ct wu h ad uIl dunnl! trans er).
Co11ce11tration:
                                                                                                                                % Mortality:                                                  n1 Mcon OfTspring/Female:                                      ~.'\
                                                                                                                                % Reduction from Control- I :                              -'\S. \"l.
CONC:          100%                                                                            S urvival a11d R eproductio11 D ata Rcolicn tc nu mber Day                                                      I            2              3              4            5            6              7              8                9                10 I              Young prod uced                        a            ('>            (\            n            0              ()          (')              ('.,              (\                0 Adult mortali ty                                                                              L
                                                                '--        L            L              '--                          L            L              '-                \_            L..
2              Young produ ced                        ()          0            0              0              0            fl            (\            0              (""\            (1 Adult mortality                      *\......      '-        '--              \..._
                                                                                                                      '--            '-              \...._
                                                                                                                                                                      '-            L              \.....
3              Young produced                        n            0              0                0          0              0            0              0            0                0 Adult mortality                          L.        L-            L            L            L                L            L            L..            L                L
      -I              Young [Jroduccd                        '-\.          s            .s
                                                                                        ~
: l.            s              ~            ~              s              Co              '-\.
Ad ul t mo rtn lity
                                                              '- -      '                            '--            "-            L              L              \...        \._...
                                                                                                                                                                                                  '--
5              Yo ung prod uced                    \~          l'-\          ' ...l        \  ,_        \'-\            \"b          ''-\              \\            '""L              J  1-Ad ult mortn li l)*                .,,            '-              '-              '-          '-            '-              '-            '-              L.-
                                                                                                                                                                                                      '-
6              Yo ung produced                        0          a              ()          0                ()            0            ()              0          aL              ('"'\
7 Adu lt mortality Young prod uced                  i..\
                                                              \__..
                                                                        \""\
L
                                                                                        \i
                                                                                          ~
                                                                                                        '2 L
                                                                                                                    \~
l--        L 10
                                                                                                                                                    \..
L"\
L..
l '\          1...0              ..,
                                                                                                                                                                                                    \......
Total youag proiluced
                                                        ~&            ~le            ~..,
                                                                                                      ~'-          31              3'\ 3'\                      ~s            ~~                ~~
Final Adult Mor tnlity
                                                            .__            '--          \._..        \...__.      \,_
                                                                                                                                        ' - '--                    '--          '---                \.._
NOii*: Adult monallly (L      11  lwc, D . d~d), SD .... split brood {tin!'JC brood spltr bc1wccn l WO d.iys:). co -  Q  n O\'Cf {offs'2rirut am cd ovt'.r with ~duh durinc. lransfcrl Co11ce111ratio11:
                                                                                                                              % Mortal ity :                                                61.
Mean O ffspring/Fema le:                                    3'o.~
                                                                                                                              % Red uction from Control- I :                          - \\ .'\ T.
SOP A Tl I - Exhibit AT I 1.2, rev ision 11 14 Page 40 of94
 
l Page 5 of?
Species: Ceriodnpl111ia dubia Client; TVA I Sequoyah N uclea r Plant, Outfall 10 1                                                                                                          Oa te: 05-02-17 CONTROL-2                                                                            Survival and Reproduction Data Replicate number Day                                                  I          2            3              4            5              6          7                B            9            10 I          Young produced                      (")      0              0              ()          ()              n          0                0            0          0 Adult mortnlity
                                                                '-        '- ' -                    '--        L                L            L.              '-          '-          '--
2          Young produced                      0          0            0              0            0             
...
...
r: I /"/' f 1trn11t norma 1t11 am 11111 ttp. ter r.etermmnt1011:
.52 3   -           20
pH of Normality Normality (N) of pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Nn 2 COJ x 0.05)/E = (N x 50000)/ 100 ml snmp le wate r reference stnndard ml ml ml =0.25/E "" NX 500 =4.SS.U. number number
*~"'
<El (:icccplnblc r.ingc = 0.0180
ro
* 0.0220)
> 0
S,..1'$ IS"i:'l-O.Y. 1'2..<Z 12..4 0 .0 'Z..t'.Y2-JO.\ a orn ory co11 o slam an; Reference st:rndnrd True vnlue Sample Alkal ini ty (MY) % RS= MY I TV x 100 number (TV) volume Begin End Totnl Multiplier (mg CaCOJ/L) (ncccplnblc range (mg CnCOJ/L) (ml) ml ml ml = 90 to 110%) ::r \JS'.:> l?S-3 100 100 In o \0. \ }\::11.0 IDl.o 0/.:> D l" I up, rcate samp. e prec1s1011:
.._
Sample Alkalinity
          ....0 l!)
%RPO= Sample Snmple iD volume Begin End Totnl Multiplier (mg CnCOJ/L) {(S
                                            --- --- ---                                           --- ---                                             --
* DJ /f{S+D)/21}
0
x 100 number (ml) ml ml ml (ncccptnblc rnngc "': 10%) s ... ' -. .:;. ;. :: '"'-:.: ' " o;.tt-\.A-SSiv0 }II() n.i *21co.\ 3.?, \o. \ 33 .*. ,. ; ... ..J Duplica1e (B) J_ l--D e;.'"' 0'5 ... 1 l1 2.lP.\ zq."+ 3.3 33 M "k atnx spt e recovery:
....t:   ...c
Reference standard Spike.value Sample Spike nl knli nity (A) number (SY) vo l ume Begin End Totnl Multiplier (mg CnCOJ/L) (mg CnCOJ/L) (ml) ml ml ml
          ....
\'33?
0 10
100 "Z.(o.. \
                                                                                *
13.'2. \0.\ 13D Sampl e all<nl n ty (B) Meas ur cu spilce value (MY) % R -MY I SV .x 100 (mg C:iCOJ/L J MV=A-B (ncccp r ablc rnngc (mg CnCOJ/L) =1 5 to 125%) 33 'i1-'1'&deg;:J'.D 0 1c s m11p, e 111ensureme11ts
* Cl.I u u
: Sample volume Begin End Total Alknlinity Snmole number Sample ID (ml) ml ml ml Multio li er (mg CaCOJ/L) 06.t)2..r:t m \+.::vJ \O'O IJ..1.5.'S
:ECl.I
: w. '2. \0.\ lq3 I o.o 1,,0 \.o \ 0505.11-.. In\> \'2...o lo.o \, \ .... --&#xa3; .\ -*lJ,-\I A rnHsw uv \ -...... \*b l.lP\.C: V1 I -;,\..\."lb*
          ....
Cl f> 2-...... . ..... I lOi> 2.4-.'l 1,.3 L,,4-os.-cn..-\\
        ....
J 3 _ ..... '2.1. 2.
0
Lt. I v'2 ', Q$c) \.<fl-I
                                                        *               *                       * *
'41>.1 3.1+ ..,, ' l o'l t\ oS<T':> I 2. I L14.() &deg;' 1_'\ 3 I J_ l.lP . n oc; as . l \o 3.\ '.'I.) ----t-3 Reviewed by: Date reviewed:
* u 0                   0             -..-..   ..---._ --- --- --- --- --- ---                                                                           --- ---
I tn-r1 Page 55 of 94 of-(v*nrt-19 t(... SOP C6 -Exhibit C6. l, revision O 1-01-13 l l l l l *1 I r .I J J I Analyst! Date analyzed DS:;;-;r
USEPA Upper PMSD Bound Acceptance Criteria(< 30%)
* ij Alkalin i ty (SM 2320 B-199 7) Matrix: Water, RL = 1.0 mg CaC0 3/L Tit ra te sa mp les to pH=4.S S.U. Page 3<i Page of 5 Time initiated F
30 USE PA
Time c o mpleted S T: I I. r l r 1frm1111orma 1 tv mu , 111 11 ltp 1 er t etermma/1 011: pliOT-r--Normality Normality (N) of pH Factor o r Mu l tiplier Deionized Titrnnt r--.. Rea i n End Total = (5 ml Na 2 C0 3
* 0.05)/E = (N x 5000 0)/ 100 ml samp l e .. water reference st:rndard ml _, =0.25/E =Nx500 "" --Cnccc"}1t:tble.i;ane:c
= 0.0180 -0.0220) =4.5 s.u. number numbe r (E) ...;..050 1o<T Lb l I a ora t ory co 11 t r o s l a m a n: Reference standard True value Sample Alkalinity (MVY % RS = MV I TV x I 00 number (TV) volume Begin End Tota l Multiplier
{mg CaC O JJ'L) (acccplnblc rnngc (mg CnCQ3/L) (ml) ml ml ml .. 90 to 110%)
100 100 -"?-. -=r-1 0.1 J O.\ l0'2.. 1 02.-, 0 Oto D r l 11p t c a le sa mv e p r ec r s 1 011: Sample Allmli n ity %RPD= Sample Samp l e ID volume Begin End Total Multiplier (mg CnCO.i/L) l(S -0) /((S+0)/21}
x 100 number (ml) ml ml ml range c:f: 1 0%) 8""" eMu f-1 lY"\ s 1**-:..-** *""[!"'-, * ,. :; *r .....
* l-"1 O.SO\.0' l to o 9 1n.) 3'1 ; :: .";. ... ' .. " _;.* Duplica1e (B) J_ .l. D 1'il*r "2.'Z-.v 3 .'\ 3q QS.O .. H:J utnx spL e recovery:
Reference stnndard Spil<ev:i l uc Sample Spike allcnli n ity (A) number (SY) volume Begin End Total Multiplier (mgCnCOJIL) (me. C:iCOJi'L) (ml) ml m l ml 5o JOD 1i.-r *).1::\-9.o l o.I '11 Snmp l e alka li nity (B) Me: 1 s u l'ed spike v:ilue (MY) % R -MY I SY x 100 (mg CnC03'L) MV=A-B (acceptab l e rnngc (me: CaCOJIL) .. 75 to 125%) 3q 52 tc't. o l>j o S I amp e m eas 11r e111e11ts:
Sample vo l ume Begin End Total A l kalinity Samole number Sam11 l e ID (ml) m l m l m l Mu l t i plier (me. CaCO.i!L l \\05.lfu. -:r t.Hv fun 2-100 ::l"l .1-;ln_R 3. \ fU.\ t\c S.C'S..\C:.. J .3 _L &>.'J 4.1.Q 32 1 1. 0S 01..o..f Ao.1 , .. D l 'JC::. 5+.o .:SS. I 1.1 l/4) .w.u. \ 1 o S o'-! .ol r 2.. I I 1.3 !< 4) 52 \I b C:..Q\o. b\ l 3 _L J.'L (.o+.) .t.\.'b n o scn.-1-4 T"tc_ 'J _ ()(")\ A-m :.>>':r.v 2.ct LA'\ 1"2.0-l\t . no S(ll...\S Ji.ilS .... s.1. 114) 1 30 * \2C\ \\ O S.<:1 2.* n_ P N\
Mto.1,1 2..q 'r ...i. '\ -\ \1 1.1 0 ftn 1$ Q ........ o.o 8.1 3.\ --\-3-,.,... .. , -z...o -1z:; 110.* ... .... Reviewed by: Date reviewed:
J 0 s.-1)1 * .-1 Page 56of94 SOP C6 -Exhibit C6.1 , revision 01-01-13 l l l l l J I _J J J J J 0 c* y* ... , * ''"' r y;..----*..) *i" 1
') LSC .)'i -, . I , .. _. ' --t.___../ ). Env l ronmrnlitl Solu\i on s. Inc. Analyst I & Date aaa l yzed Alkalinity (SM 2320 B-1997) Matrix: Water, RL = 1.0 mg CaC0 3/L Titrate samples to pH=4.5 S.U. Page 39 Page 3 of_5 __ Time initiated I':........,_ Time completed r: r l' /' l 1lra11t 11orma 1ty am mu ftp rer' ete rmma/1011:
.. ,. Normality Normality (N) pH Factor or Multiplier
.---Deionized Titrant cnci:" R .. arn End To fa I (5 mJ N11 1 C0 3 x 0.05)/E (Nx 50000)/ 100 ml sample water reference standard ml m1 0.25/E -Nx 500 -4.5 s.u. number number (E) (ncccplnb1c r: n 1'1110
* 0.0220) -.. Lb I l I a oratory coutro stmu art : Reference standard True value Sample Alkalinity (MV) % RS= MY I TV x 100 number (TV) volume Begin End Total Multiplier (mgCnCO.v'L) (acccplnblc range (mg CaCO.i/L} (ml) ml ml ml =90to110%} "f t::PS \S'S :S 100 100 3.\ 13.1.-j'D.\ Jo.I \oG..
D /' I up, 1cate samp e preCJs1011:
Snmple Alknlinity
%RPD= Sample Sample ID volume Begin End Totnl Multiplier (mg C:iCO.YL)
{(S
* 0) /((S+0)/21}
x 100 number 1V'A (ml) ml ml ml (ncccplnblc 10%) .
s l\oD -\'>*>; .. *. ... .. ... '
\ 1 Cf::lS\.1.f f\t.-F OCl 13-'2-21.0 -=1.'if 'l.) In.I . -** *--. *-* l Duplicate (B) j_ Li.) ..\_ D 2. J.o 2.c::r.o lltO 06&deg;0"1-!1-atrtx S/Jl e recovery:
Reference st:tndard Spike value Samp l e Spilce allcnlinity (A) number (SV) volume Begin End Tot11I Multiplier (mg CaCO.YL) (mgC:iCO.i/Ll (ml) ml ml ml lSS3 100 ... 50 2.)."> 13.0 :z.) 10.\ '2..1..e c -.... ,.,.,.. Snmple allrnlinity (B) Measured spike valu e (MY) % R "" MY I SV x 100 (mg CnCOJ/L) MV=A-B (acccptnblc rnngc (me CnCO.i/L)
=75 to 125%) llPO :2;,., ""'"" ') * " \OC \eo.c"'/o I s nmp. e 111eas11reme11ts:
Sample volume Begin End Total Allcnlinity Sample number Samole ID (ml) ml ml ml Multiplier (m!! CnCO.VL) r1 O.S\Th * -fJAAt r-vp 5o 'ti .. ') \'C). \ 3<..
j I Ni I L..IJ,I.\
S.K ,.,_-., l'Z-0 -\It ,
rnM1w 2.5 l),Q 3.'1 II.+) \loo \' c.s. o-i-<lS QN. c-n ho c.U. So .3 .'I -:}.o ll1. \ , , a. , 6ct
\1
\O\ \ +.t> 'l.1 L'L\
I 2. -=i.q 13.0 3 ,\ I">,'\ k '2, \4C\So'S.
\\ J 21 13*0 flD.3 .'l, (,-i.-)
1\0So\.ti.J.
'1.Jlt S'Qr-> 'Q I 1,.JT l llt*"i 14..1 l) .'l (;). '\ '5G\
\\ 0 S.<P.>*1>\o 2 --1q:i c.,,...\ -Vt Reviewed by: Date reviewed: j
-01* \" Page 57 of94 SOP C6 -Exhibit C6. l , revision 01-01-13 l l l l l ] .1 I .. .J _J J J I I _...; Analyst j f?Su Date analyzed ._f-_-_ ...
Alkalinity (SM 2320 B-1997) Matrix: Water , RL = 1.0 mg CaCO J/L Titrate samples to pH=4.5 S.U. Page Page !.-{ o f 5 ---Time initiated Time completed I ..__ ______ _, Titraut nor ma lity and multiplier tfeter111i11atio11: Pu v. Norm3lity Normality (N) of pH Factor or Multiplier Deionized Titrnnt check .,_ .. Total (5 m l Na 2 C0 3 x 0.05)/E (N x 50000)/ 100 ml sampl e --.. "' water reference st:ind:ird ml ml lnr-
=Nx500 = 4.5 s.u. number number (E) ( p b =0.0180-0.0220) ---..
Lb a oratory contra I t I I Sant art: Reference stand:ird True vnfuc Snmple Alkalinity (MY) % RS= MY I TV x 100 number (TV) volume Begin End Tot:il Multiplier (mg CnC03/L) (ncccplnblc r:ingc (mg CnC03/L) (ml) ml ml ml =90 t o110%)
100 100
=32 IT SS\-I t;j;;:,.o 0/ .. c:i.q 11.,.k tro. I I t>t> D r I up, tcate S ff l11fJ e prec1s1011:
Sample Alk:ilinity
%RPD= Sample Sample lD volume Begin End Total Multiplier (mg CnC03/L) ((S
* D) /f(S+D)/2)} x 100 number '&../IA (ml) ml ml ml (ncccplnblc range*: JO%) s -. -. ,. . **.: . ... ..... *.*t*.'"*-;:: .. ' no$ ID I 5't> 32..4 10.\ l<S . : ** .t 35.'P 3'2.. t'.l-\ '"...-. . ..... ' ...... * . :\; Duplicate (B) _L L D 35',\p
('to) (( '5 &'St-CIS*.,*Mr Jiil "k atnx spr e recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SY) volume Begin End Total Multiplier (mg (me C:tCO.VL) (ml) ml ml ml o&#xa9;ti(}-J:-NSS 155'3 lt:>O 5o 35.v Hg.''l. '0.'2.. \0.1 110 Sample allm l inity (B) Mensured spil<e v:iJue (MY) %R=MV/SV.1.JOO (mg CnC03'L) MV=A-B (:icccptablc rnngc (mg CoCO.VL) =75 t o 125%) (;5 nr IOS' Ori:' " I05'JI, -s amp, e 111ens11re111e11ts: Sample volume Begin End Tot:il Alkalinity Samp l e number Sample JD (ml) ml ml ml Multiplier (m2CnCO.i!Ll \10 SQ\.(C) t0 l 01 Uv \ So (),0 2. .'1 2.'l ,.,_, IC.\
l "l OS'S?> :6 I 2. 2. Lr.I .. !-?\ J 3 . \'"\o<":ioS. \1 le.I 't.c; 3.c+ U.\ (t>Cf \\D S C\ .6 4 l..J ASG..-NlCl
\>Jl\l\J \ C'+ J.:'55' t'a5 .,3.o l4A lt I .. ,.... -"" , .. ,
z_ 11:-? .;;>1.1 3.i..os lo5 I 4+.-5_ ('2.\ noS0'5. 3 .........
__ ,., , --
.;J.S.o 3.-1. D-1 \\ OSIR..-OS
\ 2.-q.S 11.5 z..o L?o) I ooC Y\
I '2.-l 11.c:;' 15.lo .9.. \ fc..,") 1\00 \\
! 3 J-15.S ).g I c:T>\-''--
'\Vo Reviewed by: Date rev i ewed: I O!.* en .1-\J Page 58of94 SOP C6 -Exhibit C6. l, revision 01-01-1 3 l l l l l J J l J Analyst I Sc:i v Date ana l yzed 1--o"'""s-
.-o-"'.}-*-lt----1 Alkalinity (SM 2320 B-1997) Matrix: Water, RL = 1.0 mg CaC0 3/L Titrate samples to pH=4.S S.U. Page '4-1 Page 5 of 5 ---.Time initiated Time completed r----.__ _____ __, Ti trant 11ormalit mul 11111/ti lier delermi11afio11:
De i onized Titrant water reference
= 4.5 S.U. number number L b I l I a oratory contro slam art : Reference standard True value Sample number (TV) volume Begin (mg CnCOJIL) (ml) ml ISS-3 100 100 D /' I up, tcate samp, e prec1s1011:
Sampl e Sample Sample ID volume Begin number (ml) ml 1 I Q.507.. ok:> 1::;.r\/1.1 l hlln.cJ \ 1. 35,0 l Dup!Matc (B) ..!-o.o .,, atnx sp1 <e recovery:
Reference stnndnrd Spike vnlue Sampl e number (SV) vo lum e Begin (me. CnC03'L) (ml) ml 'f }-.!SS
.::<5oo ?._ o.o Snmple allrnlinity (B) (mg CnCOJ/L} 3300 s l amp, e 111ens11re111e11ts:
End ml 35.o End ml 4J.'+
End ml 11.S Normality (N) of H 2 SO J = (5 ml Na 2 C0 3 x 0.05)/E =0.25/E pH Fnctor or Multiplier
= (N x 50000)1100 ml s ample =Nx500 range* 0.0180 -0.0220) All<alinity (MV) %RS=MV/TVx 100 Total Multiplier (mg CaC03'L) (ncccptnblc range ml = 90to110%)
11> .o fo.1 IOI \o l. o 0 (o Allcnlinity
%RPD= Total Multiplier (mg CaC03'L) {(S -0) /((S+D)/21) x 100 ml (acceptable r:in g c = :1: 10%) s ;.**** .;-
... lt .'-+ t<n\IO.l 3z.oO _...,_-..... I *o\ _l D -Ir.
* 33oO 'l:l.o!. .. f>.<;' ... .. )n... Spike alknlinity (A) Totnl Multiplier (mg CnC03/L) ml 11.'3 \t:>.I 5l?oo Measured spike vnlue (MV) % R= MV I SY x 100 MV .. A-B (ncccptnblc rnn g c (me. CnC03'L) =75 to 125%} :<5oo joO .C) 0/o Sample volume Begin End Total Alkalinity Snmple number Sample ID (ml) ml ml ml Multiplier (mg C:iC0 3'L) '\\
<[)
hOAd 2-'.2. II.$'
lD.4 )O. \
.., 1\osot...o'-l j 3 r:i.q II.'+ 3-z-.oo n os01...<J1 o4c."'\L \ ..71-l..2,
'+.o <=io' . noS&#xa3;1-t.<e>
I 2. 2.S.'; 4.'0 '2,Qoo \\ O'S.D\...Cf.;> .l 3 1-\..o F\'()) -Z.Ooo 06,0t.{.1'}
I oO t.!U.S lo.'2. IC><:> -S1 L 'b/0-o 1 Rev i ewed by: Date reviewed: I OS-CJ)-1 1 Page 59 of94 SOP C6 -Exhibit C6.l , revision 01-01-1 3 I .1 l l l l l l l _I I J J J l rfPJ ,-f *. : t *r--I ; ..._ I _; _J c_-:,./ Page } Page __ l_of 6 \-------*-* E n v!ronmtnUI Tas dng Solutlons.
Inc. Hardness (SM 2340 C-1997) RL = 1.0 mg Analyst I lu Date analyzed t I l -r . r l I 1 ran uorma 11y ant mu tip, 1er t e ermma t011: Titrant Normality check Begin End Total reference stan dard ml ml ml number number (E) 1.tJq,.--=tQ, I NsSI 1-Nl4 l).() \O.o L b I I I a oratory contro stam nn: Reference standard True vnlue Sample numbe r (TY) volume Begin End (mg C:iC03/L) (ml) ml ml \ N$S 1'-1-ef} 40 50 (D.0 \2_o D r I up. 1cate samp, e prec1s1011
: Snmple Sample Snmple ID volume Begin End number (ml) ml ml Q4*80. /). .Ssw Ob l2.o l'-t.o .! Duplicnte (B) jvlo M "Ii atnx sp1 *e recovery; Reference standnrd Spike vnluc Sample number (SV) volume Begin End (mg CnCOJIL) (ml) ml ml \JllS.S1'ltH 6D 14.u \*>,u Time initiated
..... -=<.P.__ __ ---1 Time completed
._ .... _Q""'-6l.P""'-=------'
Normality (N) of EDTA pH Factor or Multiplier
= 0.2JE = (N x 50000)/ 50 ml sa mpl e (:icccpfnblc rnngc = 0.0180-0.0220) =Nx 1000 0.0200 z_o.o Hnrdness (MV) %RS=MV/TVx 100 Totnl Multiplier (mgC:iCO.v'L)
(:iccc pr:ib l c rnngc ml = 90 to 110%) -z_o -w.o '-lo \00 01. Hnrdness %RPD= Totnl Multiplier (mg C:1CQ3/L)
{ S -D} /f{S+D)/2J) x IOO ml '2.,o Zo.o s YO : .. **'
j D 40 --41-.0 '-I 2e n-Spike hardness (A) Total Multiplier (mg CnCOJIL) ml zo.o eo Sample hnrclncss (B) Measured spike vnluc (MV) % R= MV I SV x 100 (n1g C n C:OJ/I.) MV=A-B (ncccptnblc rn11gc (mg CnCO.v'L)
=75 to 125%} '--10 yo 100. in. s l amp. e 111eas11re111e11ts:
Sample volume Begin End Total Hardness Snmole number Snmole ID (ml) ml ml ml MultioJier (mg CnCOJ/L) TV Blank 5D lA I. (should be = 0 111&#xa3; CaCO.;/L) l).Q b.O o.o w.o O'-{
'e:>.c> 2,).() "2.,o r '-JO 6E>W O"l.11a.n mi+sw
"'4.ltJ 4.1.tJ Cl2.. b Y.14.11-A J.9./ '{.-;-C/o l')'{.j q.1}8 iA'1.J j.3.-\ 66 O\,/.JltJ.11 A "3.J. '.)
J.y 01../ *9.0.11 r.... ' '-41.c -t../.1 ez.. bv
(-} 4W '-4i.8 I (.ti I v {b 41.e; O.i.o (2 J (.., 43.4 44:0 ' 12-Note: If >15m l oftitranl is used, sample must be diluted. Reviewed by: Page 60of94 Date reviewed I 6S *o\* t'\ SOP C7 -Exhibit C7.I. revision 01-0 1-1 3 l l l l l l l l l I .I J I J J Analyst I Lf'._, Date analyzed r:1 u* r l t . /' t 1 ra11 11or111a uy a11t 11111 tp, 1er t e ermma t011: Hardness (SM 2340 C-1997) RL = 1.0 mg CaC0 3/L Page 2. ---Page 2-of 3 -------Time i ni t i at e d E 01.t*2-(9p. ---i Time completed Htr..fillf Normality check Begin End Tot a l Norma l ity (N) ofEDTA pH Factor or Mult ipli er reference standard "" I .1 0.2/E =(N 50000)/ 50 ml sa mpl e number number (E) (:tcccptnb1e
=Nx 1000 v-L b I l I a oratory contro slam a n: Reference standard True value Snmple Hardness (MV) %RS=MV/T Vx 100 number (TV) volume Begin End Totnl Multiplier (mg CnCOJIL) (n cccptnblc range (mg C:iC03/L) (ml) ml ml ml = 90 to 110%) \'-it)"} 40 50 c..\"10 z_o w.0 YO l c:xJ. ({), D t: l up. 1cate sm,,-o. e prec1s1011:
Sampl e Hardness %RPD= S ample Snmple ID volume Begin End Totnl Multiplier (m g CnCOJ/L) {(S-0) /((S+D)/21} x 100 number (ml) ml ml ml Q\)Ot)Jn D 60 13.1 IY.1 1.0 s 't<J .. -io.o .. Duplicate (JU .J l <..\. t 15.1 1.0 1 D --1.1
't-<) /; atnx spi <e recoveri 1: R efe rence st:mdard Spik e vnlue Sampl e Spike hnrdness (A) n umb<?r (SV) volume B<?gin End Total Multiplier
{mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml \
'4D 6U \ ll.I l1-o 2. .t1 'ZD-0 66 Snmpl<? hnrdncss (B) Mc.1surcd spike value (MV) % R = MV I SV x 100 (mg CnCOJIL) MV=A-B (accc pcnblc ran ge (mg CaC03/L) = 75 to 125%) -Z-0 35 QS.07, s l amp. e measurements:
Sample volume Be g in End Totnl Hardne ss S:imple number Sample ID (ml) ml m l ml Mul ti pli er (mg CaC01/L) T V=Nn Blank (ShOUIU uc u 111 1!, \..llL.'-'.Yt..J 0 t1:uJ1r i; 5D 1 7,0 11*!1 o.ti l,b.o 1e I 0 I=" 11-.'l ll1.3 Z.8 1 Ill . ., 1,6 3<.o 2J. / 1-{ a. t I I. It) 32 -r a'u J. 1..f 28 -tJ. b. y "1B I K ?i:J-S 3JS Bb L ol:S 1.1 3e
* M I .
JJ.tt O,') JO Not e: If >15ml o ft itrnnt is used, s am ple must be d iluted. Reviewed by:
Page61of94 Date reviewed I oS*O\*\\ SOP C7 -Exhibit C7. I, re vision 01-01-I 3 l l l l l 1 l l J .J J J J J Analyst I vl_; Date analyzed t---O"-l.{---.-.1-5-.-j }----1 Hardness (SM 2340 C-1997) RL = l .0 mg CaC0 3/L Page _.3 __ Page 3 of_...;:3'---
Time initiated , .........._
Nl-e!lf-Time completed r:1 I Ir r 11 n . r I 1 ran norm a t 'V ant mu t/J. 1er t e ermma 1011: Titrant Normality check Begin End Total Normnlity (N) ofEDTA pH Fnctor or Multiplier re1,... stnndnrd ml ml ml 0.2/E = (N x 50000)/ SO ml samp l e number number (F.\ (ncccpt:t blc range 0.0180. 0.0220) -Nx 1 0 0 0 Laboratory co111rol stmulnrtf:
Reference stnndard True vnlue Sample Hardness (MY) % RS = MY I TV x I 00 number (TV) volume Begin End Totn l Multiplier (mgCnC O ,>/L) (ncccptnblc range (mg CnCOJ/L) (ml) ml ml ml =90to110%)
I JJSSM6} 40 50 a3.1 2..0 ZQO 40 I DO , l/J
* D r up, 1cate sample nrec1s1011:
Si1mple Hardness %RPO= Snmp le Snmple JD volume Begin End Totnl Multiplier (mg CnCOJfL) {CS* D) /f(S+D)/2(}
x 100 number (ml) ml ml ml d) lJ ll/lJ.._,..
1\J 26.'\ s .3 (o .* ,. 5D :j7'.':l 1.6 2.0 ... 0 : .. .. Duplicate (Bl) j 11.r 3q, S" i.e j D 3<.:? -o42eH atnx sot <e recovery:
Reference standnrd Spike value Sample Spike hardness (A) number (SV) volume Be gin End Total Multiplier (mg CnCOJfL) (meC:tCO.>!Ll (ml) ml ml ml (
4u 6U 3r."} c.tf .<<; w.o 70 Sample hnrdness (B) Measured spilce value (MY) % R = MY I SV x 100 (mg CnCO,>/L)
MV=A-B (:1cccptnblc rnngc (mg CnCOJIL) =75to125%)
'3cp 40 100.d>. S I amp. e 111ens11re111e11ts:
Sample volume Begin End Total Hnrdncss Snmole number Sample ID (ml) ml ml ml Mu ltiplier (mg C:iCOJIL)
TV "Rl::ink l'IU (shoul d be OmgCnCO.;/Ll
* {\I.{ 2:t31) OvW\h.._ 0 6D ao.'$"
Q.C\ a,o,,o /8 0 p I 12--() aQ.o b,!J..9-fl. a!M* O.f. 14 s o.:, ltJ.O -(" J..o.J. J..4.o o.<1 Iv v :20 \)
: b. I L*D w 3,5.7 o:i.. ti.a Not e: If >15ml oftitrant is sample must I 'ilM.'T"I I. I ) . /7 Reviewed by: f' A I Date re view ed J .n Page 62of94 .... SOP C7-Exhibit C7.I , revision 01-01-1 3 l l l l l l l _.I __] J J _J J J 0 #!a.jj-C::J 0 .t 0 :. *;:J . . ..
? j
* E n vi r on men I al T utln!J So luU on s. Inc. Pag e 4 ---Page \ of LJ. ---Hardness (SM 2340 C-1997) RL = 1.0 mg Ca CO;IL Analyst I II-' ) Date analyzed ( r I I tr C 1 rmt 11orma tfJ 1 am mu 1p rer t eter111111at1ou:
Titr:int Normality check Begin End Total reference standard ml ml ml number number (E) 0.-0 \0.0 10.0 l b I I I (1 ornto r y co11tro slam an: Refer e nce s tandar d True value Sample number (TV) volume Begin End {mg CnCO.i/L} (ml) ml ml r 'N$ l'-\<t-:r 40 50 10.0 I\. 'i D /" I up 1cate snmp. e prec1s1ou:
Sample S a mple Sample ID volume Begin End number (ml} ml ml 05.cH.(} 6Sw c;-o 4 Ouplicalc (B) _L atrix s11i <e recovery:
Reference standnrd Spike value Snmple numbe r (SV) volum e Begin End (mg CaCOJ/L) (ml) ml ml -rLI&#xa3;.<. +->-ID So r;r.w Time initiated T ime completed
..... ..._l-'4-'-'-l.
-" 3:-'-----1 Normality (N) of EDTA pH Fnctor or Multiplier
=0.2/E = (N x 50000)/ 50 ml samp l e {ncccplablc range= 0.0180 -0.0220) =Nx 1000 n O? ()0 Zo.<> Hardness (MV) %RS=MV/TVx 100 Total Multiplier (mg CnCO.VL} (ncccpt:tblc r a nge ml =90toll0%)
I. q; 2-0.o 3\C'.C Hardness %RPO= Totnl Multiplier (mg C:tCOJIL}
{(S-D} /l(S+D)/21}
x 100 ml s .. 1-"1 'Zo.o 3i .*-: .. . . D ---&#xa3;'!1<..-
).'\ .L os;-* .,..-4 Spike hnrdness (A) Totnl Multiplier
{mg C:tCO.i/LJ ml .a.q -io.o -a Sample hardness (B) Measured spil<e value (MV) %R=MV/SVx 100 (mg CnCOJIL) MV=A-B {ntcc p tn blc rn n gc (mg CnCOJIL) = 75to125%)
38 es. <>5-0 ..... y 1.f-'40 )OO.o 0 Jo s l amp, e measu rem en ts: Sample volume Be gin End Total Hardness Snmnle number SamulerD (ml} ml ml ml Multin l ier (mgCnCO.i/L)
T V=ND Blank (sho uld b e"' 0 mg CaCO;/L) 5o o.o D.o o.o -zo.o ....w (6.a;i.11-;YI ltSW .2&.\..\A 1-j.
I '2&+."' '-\-. I ()6.0S,1). .J z.g ,'}
'-\..tl. B'O l\ '1. --u..-,*H\ (n H-v..) \}., v ' 3'}.'5 i-\. !-\-egg 0'-\*'1.b-\\
& I 2 31-.5" .t.+,g 't;\p j 3 A. ...... \\Qg)\.0 \ .:je,l.l R .l5ot'"\ ) D.O 2.'l 2. _q si \ .:--...
\ 2.. ?q &:\ I.
SY.
os. *S J 3 --_l--Fl.V.* Date review ed j
\*1'1 No t e: If >15ml ofti trant is use d , sample must be dilu ted. Reviewed by: Page 63of94 SOP C7 -Exl1ibit C7.I , revision 01-0 J. IJ l l l l l l l J J J J t= m?;''i'E . c *':-. -;( , ... ,, :. i : .i "*,i ! * -I -:---_ .. I . I * ... -'-... _., Pag e S Page 2... of 4 .._) En vironm c nta1 Totln_g SofuUons: , Inc. Analyst I Hardness (SM 2340 C-1997) RL = 1.0 mg CaCO,IL Date analyzed O'O*Ov "\ Time initia t ed I "--
--i Time completed . _ Ti' I ,. r ttrnnt 11orma ity anti 11111 /tfJ, ter llelermma/1011:
...... J a ....... uu. ... litv check Begin End Tot r ll Normality (N) of EDTA pH Fnct or or Multip Ji cr reference stnndnrd ,, ml ml = 0.2/E = (N x 50000)/ SO ml sample number number ---f--41?.l (acceptable range= 0.0 l80 -0.0220) =N x 1000 own--Lb I I I I I a oraforp con ro s arl: Reference st:indnrd True value Snmp t c Hnrdness (MV) % RS= MV /TV x 100 number (TV) volume Begin End Totnl Multiplier (mg CnC O.i!L) (ncc cp t nblc range (mg CnCOJ>'L) (ml) ml ml ml =90 to 110%)
\ '--\-'&1-40 50 l:O.\.\-Jv.4 2..o 2J;>.o >+o I cc.o 0 b D r I up. tcate smm1. e pr ec 1s1 011: Snmple Hardness %RPD= Snmple S:implc ID volume Begin End Total Multiplier (mg CaCOJ/L) ((S-D) /((S+D)t21}
x 100 number (ml) ml ml ml s -* .. *. .. \'"lb SQ\ *6 'l-5o 10.4 114-.q 1-\-.5 1..o.o 'lo '. . I .. l-Duplicate (B) j_ .1-D --as, 05' .* ..,,,_ IL\-."I l'lH-'lo afrrx sp 1 re recovery:
Reference stnndnrd Spike vn luc Sample Spike h n rdnc s s {A) number (SV) volume Begin End Total Multiplier (mg CnCO,tL) (mg CnCOJ/L) (ml) ml ml ml I N5 s;, '-ten '-to So li+."1 2..1.'+ "'.'5 'Z,o . ..-:i \60 Snmple hnrdncss (B) Measured sp ike value (MV) % R = MY I SY x JOO (mg CnCO.i/L)
MY=A-B (nc ccpl:tblc rnngc (mg CnC03/L) =75to125%) 'lo J-fC 100.0&deg;/o S I amp e mens1treme11ts:
Sample volume Begin End Totnl Hnrdn css Sample number Sample ID (ml) ml m l ml Multiplier (mg CnCO.i/Ll TV=Mn Blank -61Xt1rt-(SllOlllO OC V Oil! 1...aLU,;ILJ t.. ".>" ... .,""*or. 2..l '4-25.4. 1-1-.o -z_o.o '60 l.b j 3 ....L
,2o,Q >-h1a e>--4 llr.n ,, \ C::..""1 3o.o ,,., .:2. '} :.:t Ut_ . 'i ().S.C.'\.
() \ ' 2 I '2.?.1 '1.C I 2.1! 11oso1o.o\
J 3 J_
;1. '1 \.., oScn.
* I'**\ Pi 1"-/A-r&#xa3;.()01 A-if) LO 1-W.,c;' -;J.,o -fVV "'
II> ,_ \\n'E.1'1'1..
* K, . 1'rd-thn 15 o.o 3.S 6.5 If .,,'I \4() \10S<n .;7-/\ -L Pm ('::> 3.5 ,C ,\p t. \ --6:V 't-\0 -..c:......<<
b* L 1 () <n_
* i IJ}-P"1 2.5 \0,\, 14.\ L'Z-) \tlO Note: If >ISml o ft itr:mt is used , sample must be dilutecl. Reviewed by: rt Page 64 of 94 ..__ ___ ()' ....... Date re viewed I O .S* ()'-.. (\ SOPC7-Exhib itC7.l,rev ision01-0l-13 l l l l l l l J l J J J J Page Page 6 o f Y.. Hardness (SM 2340 C-1997) RL = 1.0 mg CaC0 1/L Analyst I Sc:it..-,;v Date analyzed OCQ. ou. )'"} TiU:o.11t uormalitv aml 11111/tip/ier tletermi11ntio11:
Titrnnt Normality enc ... " "' 'h End Total -0 reference standard ml ml llll number number (E} L b I I 1 a orntori1 co11tro stnm arl: Reference standard True value Sample numbe r (TV} volume Ile.gin End (mg CnCO J/L) {ml) ml ml 40 50 1&.\-.\ \\#.0 D r l up. rcate snm p, e prec1s1011:
Sample Sample Sample ID volume Ile gin End number -r.JA (ml) ml ml \""\t.>SU'\-1.K ALF O'.J1 llf,O 1.\.1 Duplicate (B) J_ .l) .-:r 'A-:-S'" M .,, ntnx sp1 <e recoverv:
R efere nce standard Spike value Sample number (SY) volume Begin End (mg CnC03/L) (ml) ml ml :f \.JSS I..\. '?o 2.S 2qs -. Time initiated,............._
Time completed
'S Normn lit y (N) ofEDTA pH Fnctor or Multiplier 0.2/E (Nx 50000)/ 50 ml snmple (acccptnblc range= u.u.;;_ n n??O\ Nx JOOO -........ f ... Hardness (MV) % RS= MV I TV x 100 Total Multiplier (mg C:iCOJIL) (ncccptnblc range ml =90to110%) 1.q 1,,o.o 3'B Cf'5.c 0 (, Hardness %RPO= Total Multiplier (mg CnCOJIL) {I S-D) /J(S+D)/21} x 100 ml 1-'2. ci .. 5.1-,,.,'?A>.o
*o .. '. .. D..,,..,.,.
5.Z f:6v +.==f-es" 05.01.*11-'2.?)2
* Spike hardness (A) Total Multiplier (mg C:iCO J/L) ml 2.0 , , ":\ Z,O;\:> 310 .., -* ... Snmple hnrdncss (B) Mc:tsurcd spilce va l ue (MV) % R =-MV I SV x 100 (mg CnCOJIL) MV=A-B (ncccptable rnngc (ml! CnCOJIL) =75to125%) 2.30 oS*tbl'l-'OD \O\>. 0 s I amp. e 111eus11reme11ts:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mgCnCO.i/L)
T" .._TT"\ Blank
* ost1'1'} (should be= 0 mg CaCO:;/L) i\IA At&#xa3; UP 2.5 1.&f .'5 3o.v \.\ '"'"" '2n '-ti .o 44 1.'\ l 1NT J-3'0.lo 3th'2.. 3.!.?_ l.f.) l.i..lD-11-l'-l
---*-*
rnc.uiteo 5 3'5.1-a,.'6 I.\ (In"\ 2.-Z..o 11'f>C(o4.oS
(' N...rn -hi') 1?1 o 50 31n.1. IHO.C\ ""'* \ ez, -' A,.... 0 .. f vTU =-->-. l I U \ I _ _,, ...... a l,'T l 'I I . ,:'\ ' -I). ..... l I ...............
-r ... "I lo\D ., . ' lD'-+ " '\ "-I I 0 .. \-I .. I '"T0.'1 "'?.'l.V I* " I. . \ 1 c.c:.o \ .o-4
... \101 11.li \ f)o ,..,,..., 3.'2.-ll,.7-la'+ \1 o :.. '!>lo j 2 _L ;.:z. (i.?
--I.Iv Note: If >151111 oftitranl is used , sample mu s t be diluted. Reviewed b y: CT\-\"'\ d{ Date reviewed I o 1 SOP C7 -Exhibit C7. I, revision 0 1-0 l -1 J l l 1 l l l l .1 J J J I J J J :" l '<.:\'j ' L_ I ;--*, --...___./ Pag e -=1-Page 1+/- of ) Hardness (SM 2340 C-1997) RL = 1.0 mg CaC0 3/L Analyst I SSv lfJ Date analyzed l'3' Time initiated O.sot#C1-Time completed
"'4F I Titrn11t 11or111n/ity mul 11111/tiplier l etermimrtiou:
--++<<---*  
"'-,rmnlitv check Begin End Total Normality (N) ofEDTA pH Fnctor or Multiplier reference standard -** ml ml = 0.2/E ... (Nx 50000)/ 50 ml sample number number r---fr;'\ (acccplnblc r:mgc = 0.0180 -0.0220) =Nx 1000 u lb I l I n orntorv contra stnm arr.: Reference standard True value Sample Hardness (MY) % RS= MY /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (ncccptnblc rnngc (mg CnCOJ/L) (ml) ml ml ml =90 to 110%) T\JSS 40 50 lq.5 I.Cf i,o.o C\S.o 0 fi, D I 11p, icnte sample prec1s1011:
Sample Hardness %RPO= Sample Sample ID volume Begin End Total Multiplier (mg CaCOJ/L) ((S-0) /((S+D)/2)}
x 100 number llvA-(m l) ml ml ml L'"\
\ 'i:&deg; 101 4f'l4 3 5v 11.i 3!-+ t..o.o s '* .. ....... Duplicate (B) J_ l D l!6i..
JS.'2-3A J\!l ntnx sm (e recover,1:
Reference st:mdnrcJ Spike v:ilue Snmple Spike hnrclncss (A) number (SY) volume .Begin End Tolnl Multiplier (mg C:iC03/L)
IJ<.c. (mg CaCO:VL) (ml) ml ml ml 11.te-:} 1-\-0 5o rt.1 5.3 "],.o .o \O l.# -\lO S:imple hardness (B) Measured spike value (MY) % R = MV I SV x 100 (mg C:iCOJ I L) MV=A-B (acceptable r:rngc CaCOJ/L) =75 to 125%) t,i 38
<i.5.o&deg;to ltt:&{ s l amp , e 111eas11reme11ts
: Sample volume Begin End Total H:irdncss Sample number Snmple JD (ml) ml ml ml Multiplier (mg CnCOJ/L) TV=N O Blank
*'-' L n ,.... ,,..._ .. *-* ._, L 1 D'Sic \. a:> -rvA 6Qi..) , oH>u \ 5D 1':1.1 v4
' ?--Z0.3 ?..3.1.,, &,:; L,1..,,
_J 3 '12 \,. 'h ... <t -'< I .Lo noso \. c'-1 -\JltSQ.v...
\\)\ I 1 1.1#.'l
\ ?>.'2.. L..14-\ "'\
I 2-dO.I 22. q 3$ L.\4 l\OSOS* ..l 3 ..... ....-1:2.il,, q 3ff .... -::Jo I I I -C$Q/*<-J-Date reviewed I O.S*OCo"l'1 Note: If >151111 o f titront is used , sample must b e dilulcd. Reviewed by: Page 66of94 SOP C7 -Exhibit C7. I , revisi o n 01*01-13 l l l 1 l l l l l J J J J ... %d Bf;f&l tf?/01 ,:. )*... ;-* -. -. '--.... :. I ' 1 *J. -* L::...J C..-."./ '\-------..... : Env il o n nnnt.al Tc.it Ing So l ullonJ. Inc. Analyst I \.U Date analyzed l)?.01-*\1-Ha r d n es s (SM 2340 C-1997) RL = I .0 mg CaC0 3/L g Page __ _
J Time initiated I iz:J 02 Time completed Titrnut u orma lit am l 111 11/ti l ier deter111i 11 11 t io11: Titrnnt Normality check Begin End r e ference standard ml ml number number Lnborntor co11t r ol st m u l11 rtl: Reference stnndnrd Tr u e value Sample number (TV) volume (mg CnC03/L) (ml) Total ml (E) Begin m l End ml Normality (N) ofEDTA =0.2/E (ncccptnblc r:mgc = 0.0180 -0.0220) o.ow o pH Factor or Multiplier
= (N x 50000)/ 50 ml sample =Nx 1000 Hnrdness (MY) % RS= MY /TV x 100 Total Multiplier (mg CnCOJIL) (ncccptnblc rnngc ml =90 to 110%) 4 0 50 2\.<; 2..0 zo o 40 HX HI. D /" I u p /C(lfe samp e precm o 11: Snmple Hnrdncss %RPO"" Sample ID vo l ume Begin End Total Multiplier (mg CnCOJIL) {(S -D) /l(S+0)/21) x 100 number ,..,.___ (ml) ml ml ml s .. " Duplicate (B) D Matrix spike recovery: Reference stnndard Spike value Snmple Spike hnrdness (A) number (SY) v o lu m e: Begin Totnl Multiplier (mg CnCO.i/L) (mg C:iCOJIL) (m l) m l ml "'-.. Snmple hnrdness (B) Measured spike
% R = MY I SY x I 00 (m1t CnCQ3/L) MY=A-B (n cccplnblc rnngc (mg = 75 10 125%) .. s I amp. e meosuremeuts:
Snmple volume Begin End Tot:il H:irdness Snmple number Snmple ID (ml) ml m l ml Multio l ier (mg CnCOJ/L) TV=ND Blank {should be= 0 mg CaCO;/Ll 8J C,.<; C\.s o.o w: o tvo lO\ f 50 ,., ,-; :J4.v lo "2. \ z.. \ i Y*IP ,n*.&#xa3;1 \\ 3 l 3 1.2. 3.'3 (Q (p -------/ M'f' .-----,_.---Date reviewed I 6S*Gi'\1 Note: If >151111 ofti1rn111 is used , sample musi be dilute d. Reviewed by: Pa!ffl o cl , l '1'-SOP C7-Exhibit C7.l , revision 01-01-13 Page 68of94 Sequoyah Nuclear Plant Biomonitoring Ma y 02 -09 , 2017 Appendix 0 Reference Toxicant Test and Control Chart 
-u :::r::: Pimephales promelas Enviro nm en t a l T es.unq s o 1uu on1 , I nc. Chronic Reference Toxicant Control Chart Source: In-house Culture 1.2 1.0 0.8 0.6 Control Limits(+/- 2 Standard Deviations)
-----.._ ...._. __ .__. __ _ ________ ,,,,,,_._
.. ----------. * * * * * * * * * * * * * * * * * * .,,,,,.,.,,----* ,_..--------------0.4 b.O -in d'' 1.2 > ta "'C I " 1.0 Warning Limits ................... ** .. * ............................... ......................... . **.--. .. -**-**-**-**-**-...**-**-**-**--**-**-** .... .__ ..
* 0.8 * * * * * * * * * * * * * * * *
* 0.6 -* * -* * -* * -* * -* * -* * .*-**--*** ---**-**-**-**-**-**---**-**-** __. .. .. , " , .............. ' .......................... .. ................................................................ , ...... 0.4 Test date e 7-day 1Cz 5 = 25% inhibition concentration. An est imation of t h e potass i um chloride c onc entration which would cause a 25% reduction i n Pimephales growth (calculated using ToxCalc). Central Tendency (mean loga rit hmi c IC 25 c onverted to anti-logarithm ic va l ue s) ---Control Limits (mean l ogarithmic IC 25 +/- 2 stan d a rd devia ti on s converted to anti-logarithmic values) -*. -* Laboratory Warning Limi ts (mean lo g arithm ic IC 25 +/- 2 coefficent of varia tion s converted to anti-logarithm ic va lues) * .... -. . . USEPA Warning Limits (mean logar i thmi c IC 25 +/- SA_75 con ve rted to anti-logarithmic values, sA.7 5 = 75 1 h percentile o f CVs reported na tio nally by USE PA) Page 69of94 lQ CD Soknlotn.
ll'K.. ........ c Pimephales promelas Ch r onic Reference Toxicant Control Chart Source: In-house Culture Lo g 10 Conve rsi on Anti-log a rithmic Valu es (g/L KC I) co T4',t n umbe r Te st d a t e 7-day IC 25 Labo r atory C a lculated CV ToxCa l D eterm i nation 7-day IC 25 CT s CT Con t rol Lim it s Warn i n g Lim i ts (g/L KCI) CT-ZS CT+ZS CT-ZCV CT +ZCV 1 04-04-16 0.7240 -0.1402 -0.1647 0.0544 0.6844 0.5328 0.8791 0.4629 0.9689 2 04-05-16 0.7762 -0.1100 -0.1569 0.0538 0.6968 0.5440 0.8925 0.4775 0.9777 3 05-10-16 0.7510 -0.1 244 -0.1528 0.0511 0.7034 0.5559 0.8899 0.4937 0.9685 4 05-17-16 0.6409 -0.1 932 *0.15 73 0.0496 0.6961 0.5539 0.8749 0.4918 0.9530 5 06-07-16 0.6998 -0.1 550 -0.1571 0.0468 0.6965 0.5614 0.8640 0.5026 0.9370 6 06-1 4-16 0.7412 0.13 01 -0.1546 0.0451 0.7004 0.5690 0.8G23 0.5127 0.9315 7 07-12-16 0.7965 *0.0988 -0.1 5*J O 0.0460 0.7080 0.5729 0.8 7 49 0.5172 0.9437 8 07-19-16 0.6348 -0.197 4 -0.1536 0.0459 0.7021 0.5682 0.8674 0.5114 0.9376 9 08-02-16 0.7850 -0.1051 -0.1502 0.0460 0.7077 0.5726 0.8746 0.5168 0.9436 10 08-09-16 0.6703 -0.1737 -0.1517 0.0447 0.7051 0.5739 0.8664 0.5190 0.9339 11 09-12-16 0.7241 -0.1402 -0.1510 0.0433 0.7063 0.5786 0.8622 0.5255 0.9270 12 09-12-16 0.7351 -0.13 36 -0.1 500 0.0 4 21 0.7 0 80 0.5831 0.8596 0.5315 0.9222 13 10-04-16 0.7791 -0.1 084 -0.1477 0.0420 0.7117 0.5864 0.8638 0.5357 0.9254 14 11-01-16 0.7808 -0.1074 -0.1456 0.04 1 9 0.7152 0.5897 0.8674 0.5398 0.9280 15 12-06-16 0.8303 *0.0808 -0.1423 0.0433 0.7206 0.5904 0.8795 0.5399 0.9411 16 01-10-17 0.7066 -0.1508 -0.1433 0.0432 0.7189 0.5892 0.8773 0.5384 0.9392 17 02-07-17 0.7 052 -0.1517 -0.142.1 0.0426 0.72 0 9 0.5924 0.8772 0.5426 0.9378 18 0 3-07-17 0.7620 -0.1 1 8 1 -0.1362 0.0365 0.7308 0.6178 0.8646 0.5 7 61 0.9139 19 04-04-17 0.7043 -0.1523 -0.1392 0.0351 0.7258 0.6174 0.8531 0.5765 0.9012 20 OS-02-17 0.6740 -0.1714 -0.1371 0.0316 0.72 9 2 0.6305 0.8434 0.5939 0.8858 Note: 7-day I C is = 25% i nhibition c o ncen t rallo n. An e st i m ati on of the potassium chlo r ide concentrat i on th a t would ca u s e a 25% reduct io n in P l mepholes growth (calcula ted u sing T oxCalc). CT= Central tendency of the ICn values. S = Standard of the IC 1 s va l ues. Co ntro l Limi t s= Mean logarithmic IC 15 +/- 2 st and ard dev l at l ons convert ed to a nt -logarit hm ic values. Warni n g Limits= Mea n logarll hm lc I Cu +/- 2CV ors. 75 con v e rteCI to ant i*loga rlthm i c values , S,.n = Standard deviatio n correspoM i n g to the l':i' percentile ol lVs reported n ati on a ll y bv USEPA is.,.= 0.38). CV= Coe(flclenl of variation. 75th Percentile CV W a rn i n g Lim i ts CT-S A.75 CT+ S A.7S 0.4243 0.9506 0.4320 0.9679 0.4361 0.9770 0.4316 0.9669 0.4318 0.9674 0.4343 0.9729 0.4389 0.9834 0.4353 0.9752 0.4388 0.9830 0.4372 0.9794 0.4379 0.98 1 0 0.4389 0.9834 0.4413 0.9886 0.4434 0.9934 0.4468 1.0009 0.4457 0.9921 0.4469 0.9948 0.4531 1.0086 0.4500 1.0015 0.4521 1.0063 
.s= ...... 0 .... \.!) 0 ... ...... c: 0 u 0 0 c: .52 ... "' ro > .._ 0 .... t: Cl.I u :E Cl.I 0 u Cl.I ro :> .... ro ro :-e c: .........
tlO E -..c -3 0 .... l!) 0 .... ... c 0 u .... 0 .... 1.25 1.00 0.7 5 a.so 0.2 5 20 10 0 30 2 0 1 0 0 Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture ------------------------* * * -----..... .-., . ------------------------------USEPA Mi n imum Acceptance Criteria (> 0.25 mg per surviving larvae) USEPA Maxi um Coeffi c ient of Variation G u i d ance C r iter i a(< 20%) -----------------* * * * * * * -..-.. ..---._ ------------------------USEPA Upper PMSD Bound Acceptance Criteria(<
3 0%) USE PA Lower PMSD Bo u nd * ---..------___ __.....-* * * * * * * * * *
* o.:...b 'f/>b 0 ,'\.b 1.'\.b " 1.'\.b o.-">b ,_.">1:> ,"J-">b r;,1.sb c"J*\.b ">i.:'\.b
">,,:...b r;,bsb cv">b c b'">b 1Y'&deg;1 01:...1 01:...1 &..,.1 0 ,_..">1 0 o.!u 0 o.*0 r;:,s-"> r;:, b"' cb*'\J 0 1-">-r;:,1 *,. r;:,'b' r;:,'b* r;:,"J' c"J* '\.c-..,_v ">1.: r;:,V "> r;:,1.* r;:,, , c&deg;" 0 c,* Test date
* Contro l Growth , Coeffi cie n t o f Variat i on {CV) or Percent Min i mum Significant Difference (PMSD) PMSD is the percent mi n imum significant difference between the control and treatment that can be declared statistically significant.
The lower PMSD bound represents a practical limit to the sensitivity of the test me t hod and is nor a minimum acceptance criteria.
Central Tendency (mean Control G r owth. CV or PMSD) 95% Confidence I nterval (mean Co nt rol Growt h , CV or PMSD +/- 2 Stan d a r d Deviatio n s)
Control Pimepha/es promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ToxCa l Determ i nation Control Growth Control Growth CV Control Growth Test (mg/uu 1 l1l larvHI !l<I T est numbe r Test date S urv ival Mean C V MSD PMSD CT 95% Confiden ce Interval CT 95% Confidence Interval (%) (mll/ln l lla l l*rvae) (%) (%) CT*2S CT+ 25 CT *25 CT+2S 1 04-04-16 100 0.461 5.9 0.0395 8.6 0.6 125 0.4 15 0.810 5.3 0.0 10.6 ?. 04-05*16 100 0.647 1 2.3 0.1138 17.6 0.617 0.4 35 0.799 6.3 *0.8 13.S 3 05-10-16 100 0.54 1 7.0 0.0614 11.3 0.608 0.431 0.7 85 6.4 *0.2 13.1 4 05-17-16 100 0.507 1.8 0.0438 8.6 0.597 0.418 0.775 5.9 -1.0 12.9 5 06-07-16 100 0.421 5.0 0.0592 14.1 0.5 7 9 0.377 0.781 5.8 -0.7 12.4 6 06-14-16 100 0.455 3.2 0.0334 7.3 0.568 0.362 0.773 5.6 -0.B 12.0 7 07-12-16 100 0.578 8.8 0.0763 13.2 0.56 9 0.373 0.7 65 5.9 -0.5 12.3 8 07-19-16 100 0.774 10.1 0.1053 13.6 0.5 8 4 0.365 0.804 6.2 -0.4 12.7 9 08-02-16 100 0.570 3.4 0.0474 8.3 0.583 0372 0.794 6.0 -0.S 12.S 1 0 08-09-16 100 0.699 3.5 0.0827 11.8 0.591 0.379 0.803 5.8 -0.6 12.2 11 09-12-16 100 0.604 S.1 0.0532 8.8 0.592 0387 0.797 5.8 -0.4 ll.9 12 09-12-16 100 0.637 4.8 0.0520 8.2 0.5 9 5 0395 0.7 94 5.7 -0.3 11.7 13 10-04-16 100 0.588 3.2 0.0506 8.6 0.594 0.4 0 1 0.78 8 5.6 *0.4 11.5 14 11-01-16 100 0.671 7.7 0.0627 9.3 0.5 98 0.A0 7 0.7 90 5.7 -0.2 11.S lS 12-06-16 100 0.617 S.6 0.11 67 18.9 0.599 0.41 3 0.786 5.7 o.o 11.4 16 01-10*17 100 0.587 7.3 0.0629 10.7 0.600 0.414 0.786 5.7 0.0 11.3 17 02*07-17 100 0.579 11.5 0.0751 13.0 0.5 91 0.420 0.763 6.2 0.3 12.0 18 03-07-17 100 0.638 6.2 0.0662 10.4 0.590 0.421 0.759 6.2 0.4 12.0 19 04-04-17 100 0.620 5.5 0.0919 14.8 0.591 0.<1 2 2 0.761 6.3 0.6 11.q 20 05-02-17 100 0.729 5.6 0.0792 10.9 0.59 6 0.4 1 6 0.776 6.2 0.6 11.8 Nore; C on tro l S u rvlv af = USEPA min mum te.n u'eo t abUtty c11teria l. 80".4 s1.1rv1va l. "'o ntro l Me a n G rowt h:;:;: USEPA mloimutfl rest cntena 0.25 m1/surv1v ng l ilNU*.
or \'irtiJ:dor fo r contro 1 1rowth. USEPA miJ:dmum (\/
(90 1"' percenlite, < 10% M S D
* M i n i mum >i&n l ftcont d fleronce. PMSO Pcrctnt m i n imum Stgn 1 f 1 nttt difference. PMSO , ,. .a measUt of prec i sion. The PMSO t s 1'1e m 1 n11nurn percen t difte , tmce betw,en t he control and l rHrmec , t th at can be: declarf d ngn i flcitrn In 1 whote elftucm t to'l!I C l ty t Mt. Lower PMSD bound determ i ned by USEPA (10'" percon 1 1 le) > 1 2%. 'The PMSO bound rep r esents .i pr.c t icaf ttmi r to the senm lVl tv of tM te s t rM t tiod ,;,nd I J not 1 m i n.m u m 1<<epu1nce a 1 tena. Upper PMSD boond *a:eptonco criter i a dotermln e d bv USEPA (90" percen1llel
< 30%. ct'=: CP n t r."I r ia ntf*f\(\I ot th! l'OWlh , CV o r S t a ndard devi1tl0rlot t he growth , C\/ o' PMSOv lues. CT 10.7 11.7 11.7 11.3 11.6 11.2 11.4 11.5 11.3 11.4 11.2 11.0 10.9 1 0.8 11.2 11.2 11.4 11.2 ,, ' 1 1.4 Test PM50 (%) 95% Confidence Interval CT* 25 5.8 4.8 5.3 5.0 5.4 4.8 5.2 5.5 5.2 5.5 5.4 5.2 5.1 5.2 4.6 4.6 4.8 4.7 4 7 5.0 CT+25 15.7 18.6 18.0 17.6 17.8 17.6 17.6 17.6 17.4 17.2 17.0 16.8 16.6 16.4 17.8 17.7 17.9 17.7 17 9 17.8 .., ;:-""'
0 r-:-::-:i r--:-i ,,,.-...-.
* I 'I-'-' --* I *--1 ** I --. ... tnYironrMnulTf'st l ng Sc.lo1'&deg;"s.
l1K. Page I of6 Potassium Chloride Chronic Refer e nc e Toxicant Test (EPA-822-R-02-013 Method 1000.0) Species: Pimeplzales prome/a s Dilution re arntion info r111atio 11: KCI Stock lNSS number: lNSS \ S&i.o Stock preparation
: Di l ution prep (mg/L) 300 450 Stock volume (mL) 9 13.5 Diluent volume (mL) 1491 1486.5 To t nl volume (mL) 1500 1500 Test oruan;sm informati o11: Onranism source: Age: Spawn date: Hatch dates and times: Transfer vessel i nfo rmatio n: Average transfer volume: 50 g KCl/L: Dissolve 50 o KCI in 1-L Mi ll i-water. 600 750 900 18 22.5 27 1482 1 477.5 1473 1:500 1500 1500 In-house culture < 24-ho ur s old <:>'>*Ci\*\"'\
\""\OQ o<:..-o-i.-n 1050 31.5 1468.5 1500 pH= \.21.. S.U. Temperature
=
&deg;C < 0.25 mL Daily feeding aud renewa l i nf ormation:
D:i y D:itc Morning feedin g Afternoon f eedi n g Time Ana l yst Time Analyst 0 05-02-17 ()o\0 X{
u I ... " 05-03-17 ,.{
!< 2 05-04-17 D\oO C,, n ... \,I. \1 3 u 05-05-17 Ob\C \"l.l b l( 4 05-06-17 " C"l\ c; 5 05-0 7-17 0"#\0 't\ \ "!l'\C H 6 05-0R-17 Olo \<':, }t \'1.\.<.>
i\\ 7 v 09-17 PpKCICR Test N umb er: 25 Comments:
Test iuformntion:
Randomizino tem late: Incubator number and shelf lo cation: Arte mi a CHM number: CHM914 Drying information for weight determi11atio11:
Date I T im e in oven: Initial oven tern erat ur e: lDa*c_ Date I Time out of oven: O'MO*"('\
0 Final oven tern eratu re: Total drying time: Tes t in itiation , renewa l , l\fl-ISW or termin:ition batch usccJ Time An:ilyst "1..0 o'-l*u.*11 A '
"' 0'\* 11o. ,, o+t.b*f'\
& 0&'&deg;'40 \\ o'-l * ', "' ol\\5 i\
O'\t..\O At OS*Ol.*'1 o'\01-Ai CS*<n.. ,, o i "to -Control illformalion:
Acceptnnce cri t eria 0 . $20% Average we i ght per surviving larvae: 2: 0.25 mg/larvae Page 73 of94 SOP A T 21 -Exh ibit AT2 I. I , revision l 1*01-14 
'"i i
: I --I. * . ...__ Page 2 of6 ,. -, I :*-----. _ _.../ ) So I 11Uans.lr.c..
S p e cie s: P imeplz a l es pr o m e la s PpKCICR Test Number: 25 S urvi va l a n d G ro w th D a t a Day Con tro l 3 0 0 m g K C l/L 450 m g KC l/L A B c D E F G H I J K L 0 10 10 10 1 0 10 10 10 10 10 10 10 10 l /D <o I O to 10 I O 10 J o 10 10 IQ 1 a 2 lQ to (O {Q IO IQ \C) LC) lo IO 10 3 I\) (Q lb I() IC) 10 (() 10 I\) I {J IC 1 0 4 10 10 /0 JO 'O IQ /0 IO ID I() IQ IQ 5 I 'O It) ID IQ LD { () IQ ( () co IQ LO 1 0 6 J U m f{) (0 lO 10 I () 10 IQ l() lO 10 7 ft) I() I D 10 r o t o lb 1 0 ID (() 10 ., 0 A =P an weig ht (m g) Tray color code:: U:g h I-BL" .e. 15.IS j\,,}.S lb.'18 1 5.'&deg;40 1-:iOO IS.e=f IS.3s llo.18 ls. &deg;37) Annlyst: 'i--Date:
13 = ran+ Larvnc weight (mg)
?13.88 *. oe 22.cH io.'&#xa5;1 Ana l yst: lA...J citl.'5l>
Date: os.11.n-C =Larvae wc.ight (mg)= B -A H a nd cnlculntcd.
, , olo
". &?>> <..to S , t\"L '-* () 2 ,.'?..'\ Co.SC\ s. *C. c.. a1 SA"\ Ana l yst: Weight per inil i nl number of la r vae (mg) =C f In i tial n u mber of lnrvne fl.,,., oi "'6'.. 6' }-It') ..,0 (J..'V Hnnd cnlcu l:i t cd. &#xa3; " t.; \0 t ,... ,... '1) "' '? \!) "' . A1rnlysl:
o* 'V . C)' 'V' 'I;) o* t$ (). (). '()" o* Average weight per inilial Pc recur ,_ ... ..--.. ..-number of J:ir"ae (mg) re d uction r. ef .* \ '2. .'\ 1. o.
: n. & <. 0 .11..'\
-.. 0 f f rom con l rol * . *'> ("/o) .:-.: .. *-. Comment codes: c = clear , d = dead, fg = fungus , k =killed, m = missing, sk =s i ck, sm = unusually small , lg = un u sually large , d&r =decanted and returned , w = w o unded. Co mm e n ts: Page 74of94 SOPAT21 -Exhibit AT21.J , revision I J-01-14 P ag e 3 of6 EIWf ron men 1 aJ T ei l in9 SG l utJO ns., l n c. Species: Phnep!tales promelas P p K C ICR Test N umber: 25 Survival and Growth Data --Day 600 mg KCl/L 750 m17 KCl/L 900 mp KCl/L M N 0 p Q *R s T u v w x 0 10 !O 10 I O 10 JO J O 10 1 0 1 0 10 10 J 10 ID 10 ( () co IO "11{ 10 '\ t .( IQ I{) I l) 2 10 10 10 tO 10 r o ro '() 10 &deg;" 3 10 ID ID l () ID t() J 0 I() 10 '\ &deg;' 4 l#. "l-'. , .. '"' (0 LO 'D io l \.) " K & '\ !' .f 5 '6u.. 10 IQ \ '"' f '"-., ).\ "'&deg; 6 g 10 " 10 \ -,'A l , lo ----7 \ () H) \ ., , ..( t \ "" \. \.. A= Pan weight (m g) '1.u 1c;,qz. Tr:t y color code:: :=.h:qM e. l'i6Lt IS.e;.t l"i.llv IS.l.I.{ ll0.12, I IS.03 IS.le Analyst: Date: a.";}. B .. Pan + Larvae wei g ht (m g) Do.41 l.t.ll.
2o.&#xa3;fz. \'t1S IS.&=\ 2o.11 Analyst: l'f ''&deg;' 20.2e Dale: o:;_ u.1-:r C = L:irvac wei g ht (m g)= B -/\ Hand calculated. S.ii 1...4'; i.. c'-\ ".iii. 'i.8'1 q.to t.(.U \.\ :>.1.-1 Analy s t: J 'Weight per initial numll c r of larv a e (m g) =CI Initial numb e r ofl:1rvnc
,.. N
"'o t\/ $' v,O .;j Hand cnlculnted. 0 v .)..: ::;;. .).. '? Analyst: . " t)' o* o* o* 0 tr "' o* o* c;)' A\'erngc weight per initial Percent number of larv a e (mg) reductio 11 from c ontrol o.1o'2.& 1. 0.
: o. 1. (%) Comment codes: c =cle a r , d =d e ad , fg = f ungu s , k =kill e d , m = mi ss in g , s k =si c k , s m = un us u a ll y sm a ll , lg= unu s uall y lar ge , d&r = de ca nt e d a nd ret u rne d , w =w o u nd e d. Comme 11t s: Page 75 of94 SOP A T 2 l -Ex hibit A T 2 l .1 , r e v is i on l 1-0 1-1 4 0 I I L-::J r;::J 0 : . ,-. '-* -. . S p e ci es: Pim e phal es pro me l as PpKCICR Test Number: 25 S ur v i v al mu/ G ro wt h Dain D ay 1050 m g KC l/L y z AA BB 0 1 0 JO JO 10 l t. "\...\ lo 2 l.. .... !Q'" (g to 3 '-\.,,.,. , .... \D \, s 4 ,;,. v.... w.. "'.I. "'L L-5 , .... '\A '" l t "'Z... \ 6 '" '" c"' \ () \ 7 \ t:> \ 0 A= P nn weig h t (m, .ci. ,..,...,.. 'i.. Tray co l or code::
l110'i 11o.l8 Ana l yst: Daic:
B =Pan+ Lan*ae 11*eigb1 (mg) 11.ot 7 n:1ly t: """" D ntc: 05. }l .j'.}:: 6t C"' Larvae weigh I (mg)= n -A o."10 Hnn tl cn lc u l a t cd. 0 ;1'&) ... Analys t: I> .. ,. ..... Weig hl per in i tial nu m ber ofl:tr\'ne (m g) "'CI I n itia l numbe r o f lnr\'nc 0 \..' 0 Hnnd enlculatcd. <:> Analyst: ()' ()' Avcrnge weight per i n i li al l'crec n t number oflnrvnc (mg) rcducrion from con t rol (%) Co m me n t codes: c =clear , d =dead , fg =fungus, k =killed, m =missing , sk =sick, sm =u n usua ll y small , lg = u nu sually large d&r = decanted and returned, w ,,.. wounded. Co mm e 11 ts: Page4 o f6 Page 76 of94 SOP A TI 1 -Ex11ibit A T21. I , revision 1 1-0 1-14 
,_.,,.,.11o"'"'"l""'
ConUol JOO 450 600 750 9U U 10$0 >unnctrs MSO 'lil l ue i 'NISD: "*.Mtel* A 8 c D E F G H I I K l M N 0 p 0 R s T u v w x y z AA 88 111111*!......m\*tDI rl".tm.mb.,ot
,...,_ .. t ttit(,.-icJ
'*"'** ...... 10 10 IS.IS 10 10 lS.3S 10 10 !6.2S 10 10 ls.98 10 10 15.40 JO 10 13.42 10 10 15.00 10 10 15.1!9 10 10 15.35 10 10 1618 JO I 10 10 10 1530 10 8 14.54 10 10 15.92 10 9 1 51"' 10 10 14.96 10 7 l S.2* 10 7 16.12 1 0 7 1 4.9 7 10 g 15.()3 10 7 15.95 1 0 6 IM O 10 6 15.78 10 6 16.57 10 I 16.** 10 0 0.00 10 I 16 IS 10 0 0.00 0.0 7 92 M$D: 1 0.9 PMSO: Plm e pho/es promelo s C hronic Refer e n c e ToK i c a nt Te s t E PA-821-R-02-013 , Method 1000.0 Qu a l i ty Cont r ol V er i'i c at i on of D a ta Ent r y , C a lcu l a t ion s, nd S t at i st i c al An al yse s I *Patt*l*fn* a...,..,,,.
... ,.,, (rnd* Wa ltft t/S**vt,...*
1 M. ... Swvtwf*& C'a t md*"lofvwlaciori wefcht(md
... nwnbu of hrv** Imel 11umtt.1 ol tr"'n lm1l ..... _. .................... -**1ooo* .... 1tKI 22.7 8 7.63 0 7 6? 0.763 22.41 7.06 0.706 0.129 5.6 0.706 23 88 7.63 0 , 763 0.763 22.81 6.83 0.683 0 , 683 2150 6J.Q 0.6 1 0 19.34 S.9' 0 592 O.E35 10.0 0.592 21 08 6 08 o.608 0.608 23.l& 7.Z9 0.729 0.729 H.94 6.59 0.659 0.659 12.04 S.86 0586 O.!i99 80 0.5!6 2079 6.07 0.607 0.607 20.74 5.44 0.544 0.54* 2041 5.87 0.734 0.587 22.37 6.45 0.645 0*81 S.5 000 ll.BS 6.04 0.671 0.60-I 21.n 6.76 0.676 0 , 676 20.08 4.84 0.691 0.48.> 20.92 4.80 0.686 0659 6.1 19.19 4.22 0.603 0412 10.2 8 5.Z 5 0.656 O.S15 20.65 4 , 70 0.671 O.A70 1 9.7S ,,n 0.725 14.l 0.4J5 0.630 18 89 3.11 0.518 0.311 20.19 3.6? 0.603 0362 17.07 o.n 0.730 0.073 0.00 0.00 0.000 0.:10 7.l 0000 1699 0.81 0.810 0081 0.00 0.00 0000 0.000 MtlWl'tum Slc n Fic.lnt Olffe,..,,CC!
Min i mum S*snlfte*nt Difference TOll n u mber: 2S T*tt dates: ________ M_*v_o_2_-0_9_._20_1_1 M ..
w.fit\l/
Cocffhflf'll Of Wot tUOI\ r.u'"'' r*dl.kUon
""' ttu l'l\beroJ l11 rvH 11 (Gft!Ut f '"'* tml) 100.0 0.729 5.6 N ot applica b le 1 00.0 0.63 5 10.0 12.9 100.0 O.S99 1.0 17.8 9 2.5 0.628 6.4 13.8 71.S o.*18 8.9 34.4 6 2 .. S 0.395 1 8.1 4 S.9 s.o 0.039 US.8 94.7 PMSO ti a rnHuirt of tut i:iredsico Tht PM.SO b m i nimum otr(ent cllfferenct:
t>etwnn the c&deg;"trot 1nd trutrnenl that ati be decf.-rcd il1ttistieallv I n a wttole effluent tow 1 dty test Lower PMSO bound cleterMined brt USEPA ( 101h PfHC'tnt ll e) :s 1 l t-4 PMSO bound det*rmln cd by USEPA (90th pe r c:enllle).;;: 50'4. l ower and pp.er PMSO boufld\ wen de t crminud from the 1 0th 1 nd 90th pe r nntile , '''P.t t1 ety , of PMSO d:.lt;i fr om (PA's WET ll'tterbbOr.Horv Slud&deg;'
USEP".
The f owe1 PMSJ bot m d ropunef'IU
* PrKllu l llm{l lo i ansi tw l h of the test method iltid is not a m i nimum critcrii tJS!PA 20011 , 2001D. Flna l Reoon: 1 tut1rl1tDOn f Ol"J vat 1abU11y !:t.hJov of U'o\ Sho r t-te.rm Chtonie at\d Acute Who le Elt l\Jent To*icltyTes t Me t hoch ,
1 tl'ld l*Appctnd l'(. EPA*ll:J..S..01..()04
:and EPA.-321-1
*0 1..00S. us E nv lfor\men ta l Prote<t i on Agencv. OH.
St.art oa e-51212011 E nd Date: 51912017 Sample Date: Comments: Conc-m IL D*ContrOI 300 450 eoo 750 '900 1 050 Conc-m9/L D-Contro l 300 450 600 750 "900 "1050 Auxllia!l'.
Tests 1.0000 1.0000 1.0000 0 8000 0.7000 0,7000 0.1000 Mean 1.0000 1.0000 1.0000 0.9250 0.7250 06250 0.0500 2 1.0000 1 0000 1 0000 1.0000 07000 0.6000 0.0000 N-M 11a n 1 0000 1.0000 1.0000 0.9250 0.7250 0 6250 0.0500 Sta tistica l Analyses Larval F is h Growlh an d Survival Test-7 OaySu.rvlva l Test ID: PpKC l CR ID. REt>-RefTOAi1,;Cill\
Lab ID* ETS-Envir. Tes ting Sol. Samp l e T'fpe: KCL-Po t ass i um c:hlof!de Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimepha l es promelas J 4 1.0000 1 0000 1.0000 1.0000 1.0000 1.0000 0.9000 1 0000 0.7000 0.8000 0.6000 0.6000 0.1000 0.0000 Transform: Arcs l n S9uarc Root Rank 1-Talled Number Mean Min Max CV% N S 1 1m C rltlr.:ll R as p 1.4120 1.4120 1.4120 0.000 4 0 1.4120 1.4120 1.4120 0000 4 *a.oo 10 00 0 1 A120 1.4120 1.4120 0.000 4 " 8.oo 10 00 0 1 2951 1 1 071 1.4120 11 347 4 '4.00 10.00 3 1.0202 0.9912 1.1071 5.685 4 10 00 11 0 9123 0.8861 0.9912 5.759 4 1 0.00 10 00 15 0.2403 0.1588 0 , 3218 39.161 4 *o.oo 10.00 38 Statis ti c Critica l Skew Shapi r o-Will<'s Tas l i ndicates non-normal distribution (p <= 0.01) 0.87827 0 896 -0.3Be8 o* vanance cannot be connrrned Test (1-tan, 0.05! NOEC Steel's Many-One Rank Test 600 Treatments vs 0-Control Trim Level EC50 95"/. CL 0.0% 5.0% 10.0% 876 51 S34 62 920.49 20.0o/o 897.43 8483 7 949.33 Aul&deg;'S.0% 865.76 826.27 907.13 09 08 .. 07 > 0.6 " "'0.5 ,.. Cl 0 4 r-03 0.2 0.1 0 g g c ... 0 ll 0 Page 78of94 LOEC 750 c.nv TU 670.82 Trlmme d Spearm a n-Ka rber 09 08 07 :l 0.6 c &. 0 5 .. Od 03 02 0 I Dose-Response P lot 10 100 1000 10000 Dose mglL Total N umbe r 4 0 40 40 40 40 40 40 Kurt 1 96258 Stan Da l e: 5/212017 End Oa1;i* 5/9/2017 Sample Date Comments: C o n c-m IL 0-Control 300 450 600 750 900 1050 0.7530 0.6100 0.6590 0.5870 0.4840 0.4700 0.0730 2 0.7060 0.5920 0.5860 0 6450 0-4800 0.4350 0.0000 Stat i stical An a ly ses La rv:J I Fish G r owth a n d Surv i val Tc s t-7 Day Growth ID* Ppl(CtCR S;omp l A tn* RFl'&deg;-RAr Lab ETS-Env i r. Testing S ot Samp le Typ . KCL-Potasslum chloride Pro1Dco1 FWCHR-EPA-821-R-02-013 Test Spec1 s PP-Pimephales promelas 3 4 07630 0.6830 0.6090 0.7290 0.6070 0.54'50 0 6040 0.6760 04220 0.5250 0.3110 0.3620 0 0810 0 0000 T rans form: Unt ra nsformed 1-Tafled I s oto nic: Co nc*m!l'L Mea n N-M ean Mo a n t Jl i n Ma x CV% N t-Stat C ri ti cal M S D 0-Contro l 0.7288 1.0000 0.7288 0.6830 0 7630 5 578 4 *300 0.63 4 8 0.8710 0.6348 0.5920 0.7290 9.980 4 2.718 2.290 0 , 0792 *450 0.5990 0 8220 0 5990 0.5 440 0.6590 7.982 4 3.752 2.290 0.0792 *soc 0.6280 0.8617 0.6280 0.5870 0 6760 6.403 4 2 913 2.290 0.0792 750 0.4 778 0.655 0.4778 0.4220 0.5250 6.868 4 900 0.3945 0.5413 0.3945 0.3110 0.4700 18.1 4 3 4 1 050 0.0385 0.0528 0.0385 0.0000 0.0810 1 15.781 4 A uxillal"t'.
Tests S tatistic Cr it ic a l Shapiro-W i lk's Teslindicates normal distribution (p > 0.01) 0.92374 0.844 Bartlett's Test indicate$
egual variances(!!=
0.66) 0.76291 11.3449 Tes t (1*Uil , 0.05) NO EC LOE C ChV T U MS D u MSD p M SB MSE Ounnett's Test <300 00 0.07919 0.10867 0.01266 0.00239 Treatme n ts v s D-Contro l Li n crar lntcrpolot l on (Z OO R c::;am plc:;) Poin t mg IL SD 1co5* 116 29 61.66 1c10* 232.58 85.50 IC15 408.09 138.74 IC20 633.70 58.72 IC25 673.96 19.94 IC40 822.97 41.45 IC SO 912.69 18.66 95% CL (E x p) S kew 54.14 436.34 1 8870 108.28 6D2.01 1.6404 126.89 754.60 0 3100 316.33 686.56 -3.8530 613.55 747.38 0.2 701 7 15 37 958.28 0 3965 829 43 945.96
* 1 0693 0.9 0.8 07
* indica t es IC es timate le$$ than the lowest conc.en t ration =: 0.6 0.9 0.8 07 i 0.6 e o.5 Cl :04 0 ... 0.3 01 0 I 0 Page 79 of94 e c 0 0 6 c: g_ 0.5 .. 04 OJ 0.2 01 Do se-Re s pon se Plot 500 1000 D o se mg/L 1 -!all , 0.05 leve l o f s gnlllcance Me a n N-llle an 0.7288 l.0000 0.63 4 8 0.8710 0.6135 0.8419 0.6135 08419 0 4778 06556 0.3945 0 5413 0.0385 0 0528 S k.e w Ku rt 0.70843 -0.4102 F-Prob d f 0 0148 3 12 1500 Page 5 of6 . ., .* 1 S p ecies: P i m e plzal es pro me la s PpKCICR Test Number: 2 5 D ail y C!t e mi s t1 y: D ay (Analvsl identified for cnch day, pcrfom1ed pH , D.O. and cond uc t ivity measurements onl v.) 0 I 2 A n a l yst 1l1 t-.,;... IL-"'"" Co nccn tmt ion Pa r amete r : .;-'. -* Q* .. -. -. -
,_ -*" * .. *--. _.__ l. . --
pH(S.U.) g,2.\
e>.10 "t.':\Z. e.oe D O (mg/L) 't.!1 '.J. .to +..:r /l .. Y Conducfi\'ity
.. .. (1 1111hos/cm) 32..\ "* -32.6 .. * '3 t<i' I* ' . ; ; .. -_> -* C O NTR O L "A lkalinity . -r..,: *;;: -. -. ' CaCOJIL) , .. -* *: ,,,* .. --. " H:irdnc s -'.'[ , ,. --...... ;': . .. -.,. (mg CaC01/L) . ,,_. -*Te mperat ur e (0 C) ,_'\."'\ "\S. '1. ,.,1..\. \ 1."'\.l.o
"'\.&.\. p H (S.U.) 0.'1* ".l.e>j 1-\40 So,p '\.-s:1 D O (mg/L) f).._\O "+.'l 1-..d ".\A JOO mg KC l/L C n n<l nrtivity l "'* , .. eq0 ett \ . (um hos/cm) . ( . ' . -*Te m pera t ure (0 C) "1.'\.i...
'L..S."\ -u>.,.\ "l.'-\*S
\.."\*'\ pH (S.U.} e.\'5 &O<P 0()5 '.} *"' l DO (mg/L) '}.'\ 'l.ta
'+.3 /l.A 450 mg KCl/L Conducli"itY i&deg;'\O ' 10"10 . -(&#xb5;mhos/cm) \070 *i *..r_ .. .!
" Tcmpernture
(&deg;C) "l.'\.<o "'l-..o "'\. .. \.a L."'\.lo "'\)\. ,_ pH (S.U.) 0.\S e,.05 l D O (mg/L) e.o '.}lb 1\-a
"\!1 -:\..s 600 mg KCl/L Conductivity 13'10 . low . :* l320 .. 0 ..
' -. -'. -***' *Te m pera t u r e (&deg;C)
'1..."'.*
(-"\.'\-& L."\*5 t.'-\.1 'bl.,_ p H (S.U.}
1-.e4 e.o5 e.02 '+.\10 D O (mg/L) eo 8.o "\;3 Q.Q 750 mg KCl/L Cond11c1ivity 161o . .. . ; .. --... . lSLIO 16&.J "'Tcmpernture (0 C) 1,"1,.. lc. 'Lt;.o .,_"' * '-i."\* 1 \.U.-S pH (5.U.)
't.02. 1-.l61. e.03 '+.5t1 DO (mg/L) Q.0 t3J:J /l.3 .}.3 900 mg KCUL Contl11c1i\
*ity 1.,. le>\O -. -. 1e3o le20 ... . . .. ... -..._..... .
.,.. " Temperntu r e (0 C) 1..L\. lo "l. .. \. -i'-\.'4 "'\."'\.
p H (S.U.} g., ,?>
'l e&+ '+.Sf-D O (mg/L) A.I +.u 6.o 1 050 mg KCl/L Conducli\'il)'
-. . -.. ';"( ... 1l
.. ,
; (&#xb5;rnhos/cm} . . . -.. -*Temperature
(&deg;C) 1.1.\."1 "\...*-\.'\
'"l.-!-'\ 1..1.\ * .., 1.c.\. 1."\. lo Init i a l Fi n al Initial F i nal I nitial Final *Temperatures performed
:ll t he time oftest initiation , renewal or termination by the analyst identified in the Doily lnfonnnlion t a bl e l oc.-ited on Page I. ....... *nl in ity nnd hardnCEs performed by the ana lyst identified on the bench sheet specific for each ana l ysis and transcribed to thi s bench sheet by: t . ltn w iw \ Page 80of94 SOP AT21-Exhi bit AT21.I , revision 11-01-14 
. I I 0 r _,_] [_ -l r.Q o I
* i l r* I 1-: ' :--Page 6 o f6 S p ec i es: Pime pl zales p r omelas PpKCICR Test Number: 25 Day {A naly s t identified for each day. perform e d pH , 0.0. and condu c tivity measurement s o nl y.) 3 4 5 6 Ana l yst Pf:..t , ...... (<.J IA-l.l.__ v---l)V jJ ,)v Conce n t r ation Paramete r I P 7' --.r. , ;!'-. r* *. ,,,.. .;.. _,..;..; ----" I --,.:;;;_*_...,_
,_ .* . pH (S.U.) Pu"
?,.oi
"'} 0.\'5 "t.e2 'J.w DO (mg/L) -i. q ':\.:} ':l-lP ":).,A Conductivity
-.. * . .., -: . -bZ.U 311-' 32'-{ . (lam hos/cm) *-_ -r.';r_ . --* . __......,.
*
.. : -.:J --*Alkalinity
-.. --
--*-CONTROL . .. " . -. " (mg CaCO,!L) ... ...... ---_ . : *Hardness ' . --*
v---g(o ; (m!!. C aCOJIL) .. .,;_ . ; .. -*Temperat ur e ""l!\.\o "\...\..-.\
"1.A. <a '""\. "\-lo .. "\...
!>\. & (oC) pH (S.U.) M1-1.'15 ')..53 0.\0 ,r:;: ')-,\.( DO (mgfL) ?-.'1 J *<6 '\.0
/l..tl 14. \ -Conductivity , 300 mg KCl/L <0'4 \ '6'tt4 0&1 .. 0I05 >. (;t mhos/cm) -*Temperature
\."\... r "\.4 .\a C..'l* 1 '\."\* "\..'-'*'
'"1.'\.\o
"\.-{. '\ "\.'\-1...
c*q pH(S.U.) 611v.1 1.":.11.1
<(. \2-'l, Q"
'i-*62-DO (mgfL)
+.r -:J A r:.\-.i-'l.ra (t.3 Conductivity
' 450 mg I CllL l 011) h C.O h'iO \HO {&#xb5;mhos/can)
-*Temperature
""\.'\,.lo '\.'\ .1 '""L'-\* \ l,.'{.\ "'-"l*1 \...'-\*L "l!V\ "1.'\*).. c*ci pH (S.U.) 0-05 "J .'1-'l <6. \ '2.-8.dl 'lM (JI 'l...sY DO (mgfL) f.q S . .J (Oft ').ca ".). t\ 600 mg KCl/L Condncli\'ity
\320 ' i ' .. l3qo a'-( 2.0 {11mhos/cm)
\ ---, -*Tempernture L_-.\. b '1..'-l-1
'"\..'-\*
(.o L.'-\..1.... L.c.\.'\ '1.'\.-S ('C) pH (S.U.) e.os -:J.'1a '"fl.\' ':l-.50
'l , l./G\ DO (mg/L) e, o -:)._" i.o
't-d 00 \(1. 3 Conducti\*ity ., --750 mg KCl/L lS9J -
-
-
.. (&#xb5;mhos/cm) . ' " Temperature l.t.\ ':"'\
~
'"\." l-1 '"cl.1 t.."\.S L."\. '\
0 20 Lower PMSD Bo und
("C) pH (S.U.)
___
1-'l l e.oe /)..tJef 0.,\2.. "\..
                                                                                                          ---                            __.....- * - -----------
DO (mg/L) 8 , l :} .-:J.. 4i *" I.DB q_(3 'l-5" 8.o Conductivity
                                                                                                                                                    ..-
,. -..
                                                                                                                                                                *
* 900 mg KCl/L ''t?bQ l.-o\D f 0a'}o
* 10
(&#xb5;111hus/c111)
                                  *                    *                                        *                * * * *                                              *
.. * ,._ . _ .. .:., -""' -.* ! *Tcmpernture
                                                                        *
'l..'i* l ).'\ .'\ t."\. f '\."\."" "2..'\-1 \...'-\* 1..
                                    ~~-------~------- ---------- - ------------
(&deg;C) pH (S.U.) T--:&#xb5;; 'l..'1-'\ 0.\2. 11..U P> DO (mgfL) B.I +.-+-j ,'2.-r.}.u 'b.l l.O.?. Conduclivity . -' 1050 mg KCl/L (1 l mhos/cm) Z\lcO 2-\G:lf) -.. Al'-f 0 .,  
0 o.:...b 'f/>b 0,'\.b 1 .'\.b " 1 .'\.b o.-">b ,_.">1:> ,"J-">b r;,1.sb c"J*\.b ">i.:'\.b ">,,:...b r;,bsb cv">b cb'">b 1Y'&deg;1 01 :...1 01 :...1 & ..,.1 ,_..">1 0                                                                                                                                            0 0o.!u 0o.* 0<:/'~ r;:,s-"> r;:,b"' cb*'\J 01 -">- r;:,1 *,. r;:,'b' r;:,'b* r;:,"J' c"J* '\.c- ..,_v ">1.: r;:,V"> r;:,1.* r;:,,, c&deg;" 0c,*
< .... *Temperature lo 1...4* 1."\. "'\ (&deg;Cl Initial F i na l Initial Final Initia l Fi n al Initial Final ** pcraturcs performed al Liie time oftest I nitiation , renewa l or 1erminmion by the urrnlyst idt:11ti1icd i 11 the Daily Renew a l Information t able l oca ted on Page I.
Test date
inily and hardness performed by t he analyst identified on tile bench sheet specific for each analysis and transc r ibed to this bench sheet by: _ . Page 81 of 94 SOP A T21 -E x hibit AT2 I. I , revision l l-01-14 
* Control Growth, Coefficient of Variation {CV) or Percent Minimum Significant Difference (PMSD)
-u nJ z _, .........
PMSD is the percent mi nimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is nor a minimum acceptance criteria.
0.0 -"' N u > nJ "O I " Ceriodaphnia dubia Envlronmcmt411 Tostlng Solution s, , I n c. Chronic Reference Toxicant Control Chart Source: In-house Culture 1.08 1.06 1.04 1.15 1.10 1.05 1.00 Control Limits (+/- 2 Standard Deviations)
Central Tendency (mean Control Growth. CV or PMSD) 95% Confidence Interval (mean Control Growth, CV or PMSD                                    +/- 2 Stan dard Deviations)
------...  
 
-----_,.,.-.------------......._._.
Pimepha/es promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture
-----* . * * * * * * * * * * * * * * *
~
* o * '" * .. * '" * * * * *
ToxCa l Determination                                                      Control Growth                              Control Growth CV                            Test PM50 Control                      Control Growth                              Test                                    (mg/uu1l1l larvHI                                    !l<I                                  (%)
* o * " ' ' o o <f I
Test number        Test date            Survival                  Mean                  CV              MSD            PMSD                CT            95% Confidence Interval          CT          95% Confidence Interval            CT  95% Confidence Interval
* o * ' * * * *
(%)              (mll/lnlllal l*rvae)        (%)                              (%)                                CT
* 2S        CT+ 25                        CT *25              CT+2S              CT
* 25    CT+25 1              04-04-16              100                      0.461                5.9            0.0395          8.6              0.6125              0. 415        0.810          5.3            0.0                 10.6    10.7      5.8        15.7
      ?.             04-05 *16              100                      0.647                12.3            0.1138          17.6              0.617              0.435          0.799          6.3            *0. 8                13.S    11.7      4.8        18.6 3              05-10-16              100                      0.541                7.0            0.0614          11.3              0.608              0.431          0.785          6.4            *0.2                13.1    11 .7    5.3        18.0 4              05-17-16              100                      0.507                1.8            0.0438            8.6              0.597              0.418          0.775          5.9            -1.0                  12.9    11.3      5 .0        17.6 5              06-07-16              100                      0.421                5.0            0.0592          14.1              0.579              0.377          0.781          5.8            -0.7                12.4    11.6      5.4        17.8 6              06-14-16              100                      0.455                3.2            0.0334            7.3              0.568              0.362          0.773          5.6            -0.B                12.0    11 .2    4.8        17.6 7              07-12-16              100                      0.578                8.8            0.0763          13.2              0.569              0.373          0.765          5.9            -0.5                12.3    11.4      5.2        17.6 8              07-19-16              100                      0.774                10.1            0.1053          13.6              0.584              0.365          0.804          6.2            -0.4                12.7    11 .5    5.5        17.6 9              08-02-16              100                      0.570                3.4            0.0474            8.3              0.583              0372          0.794          6.0            -0.S                12.S    11.3      5.2        17.4 10              08-09-16              100                      0.699                  3.5            0.0827          11.8              0.591              0.379          0.803          5.8            -0.6                12.2    11.4      5.5        17.2 11              09-12-16              100                      0.604                  S.1            0.0532            8.8              0.592              0387          0.797          5.8            -0.4                ll.9    11.2      5.4        17.0 12              09-12-16              100                      0.637                4.8            0.0520            8.2              0.595              0395          0.794          5.7            -0.3                11 .7    11 .0    5.2        16.8 13              10-04-16              100                      0.588                3.2            0.0506            8.6              0. 594            0.401          0.788          5.6            *0.4                11 .5    10.9      5.1        16.6 14            11-01-16              100                      0.671                  7.7            0.0627            9.3              0.598              0.A07          0.790          5.7            -0.2                11.S    10.8      5.2        16.4 lS            12-06-16              100                      0.617                  S.6            0.1167          18.9              0.599              0.413          0.786          5.7              o.o                11.4    11.2      4.6        17.8 16            01-10* 17              100                      0.587                  7.3            0.0629          10.7              0.600              0.414          0.786          5 .7            0.0                11.3    11.2      4.6        17.7 17            02*07-17                100                    0.579                11.5            0.0751          13.0              0.591              0.420          0.763          6.2              0.3                12.0    11 .4    4.8        17.9 18            03-07-17                100                    0.638                  6.2            0.0662          10.4              0.590              0.421          0.759          6.2              0.4                12.0    11 .2    4.7        17.7 19 20 04 17 05-02-17 100 100 0.620 0.729 5.5 5.6 0.0919 0.0792 14.8 10.9 0.591 0.596 0.<122        0.761          6.3              0.6                11 .q    ,, '
11.4 4 7        17 9 0.416          0.776          6.2              0.6                11.8              5.0        17.8 Nore ;      Con trol Survlvaf = USEPA min mum te.n u'eotabUtty c11teria l. 80".4 s1.1rv1val.
          "'ontrol Mean Growth:;:;: USEPA mloimutfl rest cc.ee1na~fii 1 y cntena ~ 0.25 m1/surv1v ng lilNU*.
OJ ~ Cof!ffte~m      or \'irtiJ:dor for contro11rowth.
1 USEPA miJ:dmum (\/ g ukf~lltt c,1t~ria (90 "'      percenlite, < 10%
MSD
* Minimum >i&nlftcont d fleronce .
PMSO      Pcrctnt minimum Stgn1f1nttt difference.
PMSO ,,. .a measUt of ~e n prec ision. The PMSO ts 1'1e m1n11nurn percent difte,tmce betw,en the control and lrHrmec,t that can be: declarfd stau~Outly ngniflcitrn In 1 whote elftucmt to'l! IClty tMt.
Lower PMSD bound determined by USEPA (10'" percon11le) > 12%.
                                                            'The bw~' PMSO bound rep resents .i pr.cticaf ttmir to the senmlVltv of tM te st rMttiod ,;,nd IJ not 1 min.mum 1<<epu1nce a 1tena .
Upper PMSD boond *a:eptonco criteria dotermlned bv USEPA (90" percen1llel < 30%.
ct'=: CPntr."I ria ntf*f\(\I ot th! l'OWlh, CV o r PMSOv~lu" .
s~  St andard devi1tl0rlot the growth, C\/ o' PMSOv lues.
                                                                                                                                                                                                                                                                .., ~
11'!--
                                                                                                                                                                                                                                                                    ;:-""'
 
0  r-:-::-:i  r--:-i ,,,.-...-.
* I  'I -'-' -  1 - **    I **--                                                                                                                            Page I of6 I    -                      -.
... tnYironrMnulTf'stlng Sc.lo1'&deg;"s. l1K.
Potassium Chloride Chronic Reference Toxicant Test (EPA-822-R-02-013 Method 1000.0)
Species: Pimeplzales prome/as PpKCICR Test Number: 25 Dilution re arntion infor111atio11:                                                                          Comments:
KCI Stock lNSS number:                              lNSS \ S&i.o Stock preparation:                                  50 g KCl/L:
Dissolve 50 o KCI in 1-L Mi ll i-        water.
Di lution prep (mg/L)              300    450        600            750            900            1050 Stock volume (mL)                    9      13 .5      18            22.5            27            31.5 Diluent volume (mL)                1491  1486.5      1482          1477.5          1473          1468.5 Totnl volume (mL)                  1500    1500      1:500          1500            1500          1500 Test oruan;sm informatio11:                                                                                  Test iuformntion:
Onranism source:                                    In-house culture                                        Randomizino tem late:
Age:                                                < 24-hours old                                          Incubator number and shelf location:
Spawn date:                                                                                                  Artemia CHM number:                CHM914 Hatch dates and times:                              <:>'>*Ci\*\"'\  \""\OQ      ~                        Drying information for weight o<:..-o-i.-n        ~t.ooS                determi11atio11:
Transfer vessel information:                        pH=        \.21..                          S.U.        Date I Time in oven:
Temperature =              "l..~.C\        &deg;C          Initial oven tern erature:            lDa*c_
Average transfer volume:                            < 0.25 mL                                              Date I Time out of oven:      O'MO*"('\      0 Final oven tern erature:
Total drying time:
Daily feeding aud renewal information:
D:i y                D:itc        Morning feedin g                Afternoon feedi ng              Test in itiation, renewa l,        l\fl-ISW or termin:ition            batch usccJ Time        Ana lyst            Time            Analyst          Time          An:ilyst 0              05-02-17          ()o\0            X{
                                                                  ...
                                                                                \'1.~o              u            o~ "1..0            ~    o'-l*u.*11 A I              05-03-17          c~              ,.{            \~c.c              !<"            09~'-\            '    0'\* 11o. ,, ~
                                                                                                                                    ~"'
                                                                                                        \1 2
                                                                ~1
                                                                                                    \,I.
05 17        D\oO C,,                        n . . o~                u o<;;.~c=\                o+t.b*f'\ &
3              05-05-17          Ob\C            ~l              \"l.l    b        l(              0&'&deg;'40          \\      o'-l
* i.~ . ' , "'
4              05-06-17          C"l\ c;          "              \~\<,.            ~l              ol\\5            i\      O~*Cfl..*\1 5              05-07-17          0"#\0            't\            \ "!l'\C          H              O'\t..\O          At      OS*Ol.*'1 6              05-0R-17            Olo \<':,
v
                                                                }t                \'1.\.<.>      i\\
                                                                                                    -
o'\01-          Ai        CS*<n.. ,,
                                                                                                                                      ~\
7              05-09-17                                                                              o i "to
                                                                                                                                          -
Control illformalion:                                                            Acceptnnce criteria 0 .                        $ 20%
Average weight per surviving larvae:                                              2: 0.25 mg/larvae Page 73 of94                                                                                                SOP AT21 - Exh ibit AT2 I. I, revision l 1*01 -14
 
    ~ r? ~ (;::i                                                                                                                                                                                Page 2 of6
: I --
          ,.            I.        *. -,...__
'"i    ---
I
                          -
: *-
_
                                    . _.../
EnvlronrTtt.nt~I Tc~Ung SoI11Uans.lr.c..
i  )
Species: Pimeplzales prom elas                                                                                            PpKCICR Test Number: 25 S urvival an d Growth Data Day                                        Con tro l                                          300 mg KC l/L                                  450 mg KCl/L A          B            c                  D              E        F        G              H        I            J      K        L 0
10        10            10                  10            10      10        10            10      10            10      10      10 l
                                                                  /D          <o            IO              to            10        IO        10          Jo      10            10      IQ      1a 2
t~          lQ            to                (O              {Q      IO        IQ          \C)        LC)            lo    IO        10 3                                                                                                            (()
I\)        (Q              lb                  I()          IC)      10                    10          I\)          I {J  IC      10 4
10        10                /0              JO                'O    IQ          /0          IO        ID          I()      IQ        IQ 5
I 'O        It)            ID                  IQ          LD    { ()      IQ          (  ()    co            IQ      LO      10 6
JU          m              f{)          (0                lO        10        I ()        10        IQ            l()    lO        10 7                                                                                                                                                                ., 0 ft)        I()          ID                    10            ro      to        lb            10        ID            ( ()    10 A = Pan weight (mg)
Tray color code::          U:g h I- BL" .e.                15.IS    15.~ j \,,}.S                    lb.'18          15 .'&deg;40 l3 .~2 1-:iOO            IS.e=f Annlyst: 'i--                                                                                                                                                    IS.3s llo.18            \4 .~z. ls. &deg;37)
Date: OY* Z:Z-1~
13 = ran + Larvnc weight (mg)
Ana lyst:                lA...J                          ~~.le J.~.'-ll ?13.88                    ~,,a, citl.'5l> ltt.~4    ~ *.oe      ~.16 ia1 .q~          22.cH io.'&#xa5;1        ~o.1~
Date:                  os.11 .n-C = Larvae wc.ight (mg)= B - A Ha nd cnlculntcd.                                          h.Co~    , ,olo      1 . 1..~            ". &?>> <..to S,t\"L '-* () 2 , .'?..'\ Co.SC\ s. C. c. . a1 SA"\
Ana lyst:
                                      ~
Weight per inil inl number of la r vae (mg)
    =C f In itial number of lnrvne It')
                                                                          ,...~ ,...~                          fl.,,.,                              oi          ~                      ~        6'    }-
Hnnd cnlcu l:i tcd.                                          &#xa3;                                                            ..,0
                                                                                                                                      '?
(J..'V
                                                                                                                                                  .
                                                                                                                                                  \!)
                                                                                                                                                              "        "'
                                                                                                                                                                          "'6'..      .
t.;    \0      ~
t
                                                                                                          '1)
A1rnlysl:                                                  ().      ~*          () .              '()"                o*"'      o*        'V            C) '    'V'        'I;)      o*      t$
                                                                                                      ,_ ...      . ..-
r.
                                                                                                              ..--
Average weight per inilial                  Pc recur number of J:ir"ae (mg)                      red uction from con lrol    0 .11..'\          l~-t~~'
                                                                                      *.
ef
                                                                                                -Ll~.* .. *'> 0f            o . b~S                \ '2. .'\ 1.          o.    ~a.Ci        n . &<.
("/o)                                .:-.:              *~*
                                                                                                        *-.            .
Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk =s ick, sm = unusually small, lg = un usually large, d&r = decanted and returned, w = wounded .
Comments:
Page 74of94                                                                                                                      SOPAT21 - Exhibit AT21.J , revision I J-01-14
 
Page 3 of6 EIWfron men1aJ Teilin9 SGlutJOns., lnc.
Species: Phnep!tales promelas                                                                      PpKCICR Test Number: 25 Survival and Growth  - -          Data Day                                  600 mg KCl/ L                      750 m17 KCl/L                                  900    mp KCl/L M        N      0        p      Q
* R            s        T          u          v        w        x 0
10      !O      10      IO    10          JO          JO        10          10          10        10        10 J
10      ID        IQ      I{)    10          I l)      (  ()      co          IO        "11{        10      ' \ t .(
2
                                                                                                                                                          &deg;" '*~
10      10      10      tO        10        ro        ro        '()          10                            ~
3 10      ID      ID      l ()    ID      t()        J  0      I()        10          ~        '\
4 (0      LO      'D    io        l \.)
                                                                                                              \~
                                                                                                                      ~
l#.      "l-'.
                                                                                                                                                &
                                                                                                                                                  ,.
                                                                                                                                                          '\
                                                                                                                                                                            &deg;'
                                                                                                                                                                    !'*~ .f'"'
5                          '6u.. 10      ~~    IQ
                                                                                                    ~        "&~
                                                                                                                        ~'"'
K f            .,'"-      ).\
                                                                                                                                                                              ~
6                          g                        10        \
                                                                                                  \
                                                                                                          -,'A l                ~
                                                                                                                                              ,          "'&deg;        ~
                                                                                                                                                                      ~
                                                                            "                                                                            ~
10                                                                                                      lo 7
                                                    - -
                                                              ~      \ ()    ~      H)        \          ., ,          ..(
t
                                                                                                                                        - -
                                                                                                                                                \                  \.      \..
A = Pan weight (mg)                        '1.u                                                                                                          ""
Tr:ty color code: :          :=.h:qM            e.      l'i6Lt  1c;,qz. IS.e;.t                                                        15.&#xa3;:\~ is. ~o      IS.le lto.6~
Analyst:            ~                                                              l"i.llv IS.l.I.{ ll0.12,      I "~:t IS.03 Date:          o~. a.";}. ~r"l B .. Pan + Larvae weight (mg)
                              ~                          Do.41 a~ .3l              l.t.ll. ~o.oe    2o.&#xa3;fz.                20.2e      a..o.~      \'t1S IS.&=\ 2o.11 Analyst:
Dale:                      o:;_      u.1-:r                                ~1 .~8 l'f ' &deg;'
C = L:irvac weig ht (mg)= B - /\
Hand calculated.                                        S.ii 1...4'; i.. c'-\ ".iii. 'i.8'1          q.to t.(.U              s.t~        ~.10 4 .~~ ~.            \.\ :>.1.-1 Analyst:
'W eight per initial numllcr of larva e (mg)
J
= CI Initial number ofl:1rvnc                                  ,..                                                      N                              ~,,,,
                                                                                                ~                              <,,~
t\/
Hand cnlculnted.                                            ~        $'      0
                                                                                ~      ~                  v,O      ~"'
                                                                                                                                              "'o
                                                                                                                                            .)..      ~
                                                                                                                                                                    ~      .;j
                                                              .                                        ::;;.
                                        ~
v      .)..:
                                                                    ~                                                                                            '?
Analyst:
                                                                            "              o*        o*          t)'        o*          ~r          o*                  o*
A\'erngc weight per initial                  Percent 0        ~*      tr
                                                                                    "'                                                                        c;)'
number of larva e (mg)                      reductio11 o.1o'2.&          r~ . ~ 1.        0 . 1.\1~                ~"\.~1.              o. ~'15                ~s . C\ 1.
from control
(% )
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = mi ssing, sk = sick, sm = un usually small ,
lg = unusually large, d&r = decanted and returned, w = wounded.
Comme11ts:
SOP AT2 l - Exhibit AT2 l . 1, revision l 1-0 1-14 Page 75 of94
 
0 0
,-
I '-
* L-::J r;::J.
  .
:
I
              .
                -.
Page4 of6 Species: Pimephales promelas                                                                    PpKCICR Test Number: 25 S urvival mu/ Growth Dain Day                                      1050 mg KCl/L y            z          AA          BB 0
10            JO            JO        10 l                          ,~ ,~                      t. "\...\ lo'4~
2 l..
                                                                                    ....      !Q'"          (g        to 3                          '-\.,,.,. s
                                                                                                  ,....                \,
                                                                                                                \D 4                              ,;,.        v....          w..        "'.I.
                                                                                  "'L          ~            ~        L-
                                                                                      ,....
5 l
                                                                                                '\A t                '"
                                                                                                              "'Z...    \*~
                                                                                                  '" '" c"'
6
                                                                                  \          ()                \
7
                                                                                    \        t:>              \        0 A = Pnn weigh t (m,                                                  .ci.              ,..,...,.. 'i..
l~l+ &sve.,                              ~*"'
Tray color code::
Ana lyst:        ~
l110'i ~ 11o.l8 ~
Daic:      CH . ~~-n B = Pan+ Lan*ae 11*eigb1 (mg) 11.ot                        t~'lll ~~
n:1ly t:
Dntc:          """"
: 05. }l .j'.}::                                  7                              6t C "' Larvae weigh I (mg)=    n- A o."10 Hnn tl cn lc ula tcd.                          0 ;1'&) ~ i."'                            ~
                                                                                                                            ,.........
Analys t:                  ~\                                      I>..
Weighl per in itial nu mber ofl:tr\'ne (mg)
                              "' CI Initia l numbe r of lnr\'nc Hnnd enlculatcd.
                                                                                ~,..,,        0              \..'
                                                                                                            <:>
0 Analyst:
                                                        ~                    ()'                        ()'
Avcrnge weight per inili al      l'crec nt number oflnrvnc (mg)              rcducrion o . o~t:\                    ~'{.11.
from con trol
(%)
Comment codes: c =clear, d = dead , fg =fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg = unusually large d&r = decanted and returned, w ,,.. wounded.
Comme11ts:
Page 76 of94                                                                                  SOP ATI 1 - Ex11ibit AT21. I, revision 11 14
 
Plmepho/es promelos Chronic Refere nce ToKica nt Test EPA-821-R-02-013, Method 1000.0 Quality Control Veri' ication of Data Entry, Calcula tions, nd Statistical Analyses TOll number:                                                    2S
                                                                                                                                                                                                                                                                                          ~-----------~
T*tt dates: _ _ _ _ _ ___M_*v_o_2_-0_ 9_._20_1_1
,_.,,.,.11o"'"'"l""'    "*.Mtel*  111111* !......m\*tDI
                                          '*"'**              ......
rl".tm.mb.,ot ,...,_ ..tttit(,.-icJ    I
* Patt* l*fn*
wefcht(md                    ...
a...,..,,,. ...,.,, (rnd
* Waltftt/S**vt,...* 1 M. . .. wt l ~/ Swvtwf*&
nwnbu of hrv** Imel 11umtt.1 ol tr"'n lm1l      ~  ..... ....................
C'at md* "lofvwlaciori
_.
                                                                                                                                                                                                - **1ooo*....1tKI W*l &h1/~1lljn~***
l~(lftfl M.. 1't1H&#xa5;1"~(l') ~~aJ'i  w.fit\l/ ~ftut Cocffhflf'll Of Wot tUOI\
ttul'l\beroJ l11 rvH tml) 11 r.u'"'' r*dl.kUon " " '
(Gft! Utf '"'*
A                  10                10            IS. IS                  22.78                    7.63                0 76?                                                                            0.763 8                  10                10            lS.3S                  22.41                    7.06                0.706                                                                          0 .706 ConUol                                                                                                                                                                0 .129                    5.6                                        100.0                0.729                    5.6              Not applica ble c                  10                10            ! 6.2S                  23 88                    7.63                0,763                                                                          0 .763 D                  10                10            ls.98                  22.81                    6.83                0.683                                                                          0 ,683 E                  10                10            15.40                  2150                      6J.Q                0. 6~C!,__                                                                      0.6 10 F                JO                10            13.42                  19.34                    S.9'                0 592                                                                          0.592 JOO                                                                                                                                                                  O.E35                    10.0                                        100.0                0.635                  10.0                      12.9 G                  10                10            15.00                  21 08                    6 08                o.608                                                                          0.608 H                  10                10            15.1!9                  23 .l&                    7.Z9                0.729                                                                          0.729 I                  10                10            15.35                  H .94                    6.59                0.659                                                                          0.659 I                  10                10            1618                    12.04                    S.86                0586                                                                            0.5!6 450                                                                                                                                                                  O.!i99                      80                                        100.0                O.S99                    1 .0                    17.8 K                  JO        I      10            14~7:?                  2079                      6.07                0.607                                                                          0 .607 l                  10                10            1530                    20.74                    5.44                0 .544                                                                          0.54*
M                  10                8            14.54                  2041                      5.87                0.734                                                                          0.587 N                  10                10            15.92                  22.37                    6.45                0.645                                                                          000 600                                                                                                                                                                  081                        S.5                                        92.5                0.628                    6.4                      13.8 0                  10                9            151"'                  ll.BS                    6.04                0.671                                                                          0.60-I p                  10                10            14.96                  21.n                      6.76                0.676                                                                          0,676 0                  10                7            l S.2*                  20.08                    4.84                0.691                                                                          0 .48.>
R                  10                7            16.12                  20.92                    4.80                0.686                                                                          o.~
750 s                  10                7            14.97                  19.19                    4.22                0.603 0659                        6.1 0412 71.S                o.*18                    8.9                      34.4 T                  10                g            15.()3                  10.28                    5.Z5                0.656                                                                          O.S15 u                  10                7            15.95                  20.65                    4,70                0.671                                                                          O.A70 v                  10                6            IMO                    19.7S                    ,,n                  0.725 14.l 0.4J5 62..S                0 .395                  18.1 9UU                                                                                                                                                                  0.630                                                                                                                                          4S.9 w                  10                6            15.78                  18 89                    3. 11                0 .518                                                                          0.311 x                  10                6            16.57                  20.19                    3.6?                0.603                                                                          0362 y                  10                I            16.**                    17.07                    o.n                  0.730                                                                          0.073 10$0 z                  10                0            0 .00                  0.00                    0.00                0.000 0.:10                      7.l 0000 s.o                0.039                  US.8                      94 .7 AA                  10                I            16 IS                  1699                      0.81                0.810                                                                          0081 88                  10                0            0.00                    0.00                    0.00                0000                                                                            0.000
>unnctrs MSO 'lillue i          0 .0 792                                M$D :                MtlWl'tum SlcnFic.lnt Olffe,..,,CC!
'NISD:                          1 0.9                                  PMSO :                P-~rcont  Minimum S*snlfte*nt Difference PMSO ti a rnHuirt of tut i:iredsico Tht PM.SO b ~ h e minimum otr(ent cllfferenct: t>etwnn the c&deg;"trot 1nd trutrnenl that ati be decf.-rcd il1ttistieallv s ift n ifk~t1I In a wttole effluent tow1dty test Lower PMSO bound cleterMined brt USEPA ( 101h PfHC'tntlle) :s 1l t-4 Up~r PMSO bound det*rmlncd by USEPA (90th perc:enllle) .;;: 50'4.
l ower and pp.er PMSO boufld\ wen detcrminud from the 10th 1nd 90th pernntile, '''P.t t1 ety, of PMSO d:.lt;i from ( PA's WET ll'tterbbOr.Horv Varl~bllilV' Slud&deg;' ~USEPA, 2001~ USEP". 2001b~
The fowe1 PMSJ botmd ropunef'IU
* PrKllul llm{l lo th~ i ansitwlh of the test method iltid is not a minimum ~:epunc:e: critcrii tJS!PA 20011, 2001D. Flnal Reoon: 1tut1rl1tDOnfOl"J vat1abU11y !:t.hJov of U'o\ Short-te.rm Chtonie at\d Acute Whole Elt l\Jent To*icltyTest Me thoch, V~umos 1 tl'ld l*Appctndl'(. EPA*ll:J..S..01..()04 :and EPA.-321-1 *0 1..00S. us Envlfor\mental Prote<tion Agencv. Cln i:lnn~t*, OH .
 
Sta tistica l Analyses Larval Fish Growlh and Survival Test-7 OaySu.rvlva l St.art oa  e-  51212011                  Test ID: PpKClCR                    S;j111&#xb5;1 ~ ID.          REt>- RefTOAi1,;Cill\
End Date:        51912017                Lab ID* ETS-Envir. Testing Sol.      Sample T'fpe:            KCL-Po tassium c:hlof!de Sample Date:                              Protocol: FWCHR-EPA-821-R-02-013 Test Species:                PP-Pimephales promelas Comments:
Conc-m IL                        2            J        4 D*ContrOI        1.0000    1.0000      1.0000    1 0000 300 1.0000          1 0000      1.0000    1.0000 450      1.0000    1 0000      1.0000    1.0000 eoo 0 8000          1.0000      0 .9000  1 0000 750      0.7000    07000      0.7000    0.8000
              '900 0,7000        0.6000      0.6000    0.6000 1050      0.1000  0 .0000      0.1000    0.0000 Transform : Arcsln S9uarc Root              Rank      1-Talled              Number Total Conc-m9/L        Mean      N -M11an    Mean        Min      Max    CV%          N      S 11m    Crltlr.:ll            Ras p  N umber D-Control        1.0000  1 0000      1.4120    1.4120  1.4120    0.000        4                                              0      40 300      1.0000  1.0000      1.4120    1.4120  1.4120    0000        4
* a.oo      10 00                    0      40 450      1.0000  1.0000      1A120    1.4120  1.4120    0.000        4        " 8.oo      10 00                    0      40 600      0.9250  0.9250      1 2951    1 1071  1.4120  11 347        4        '4.00      10.00                    3      40 750        0.7250  0.7250      1.0202    0.9912  1.1071    5.685        4        ~ 0 . 00    10 00                    11      40 "900      06250    0 6250      0 9123    0.8861  0.9912    5.759        4        10.00      10 00                    15      40 "1050        0.0500  0.0500      0.2403    0. 1588 0 ,3218    39.161        4
* o.oo      10.00                  38        40 Auxllia!l'. Tests                                                              Statistic                Critica l              Skew      Kurt Shapiro-Will<'s Tasl indicates non-normal distribution (p <= 0.01)            0 .87827                  0 896                -0.3Be8  1 96258 Eguaht~ o* vanance cannot be connrrned H~Eot hesis Test (1-tan, 0.05!            NOEC      LOEC      c.nv      TU Steel's Many-One Rank Test                  600      750    670 .82 Treatments vs 0-Control Trlmmed Spearma n -Karber Trim Level        EC50            95"/. CL 0.0%
5.0 %
10.0%      876 51    S34 62      920.49                                      10 ..-~~~~~~~~~~~-,
20.0o/o    897.43    84837      949.33 09 Aul&deg;'S.0%      865.76    826.27      907.13 08 07
:lc  0.6
                                                                                    .
                                                                                    &. 0 5
                                                                                    ~ Od 03 02 0 I 10      100    1000      10000 Dose mglL Dose-Response Plot 09 08
                    .
                    >
07
                    ~    0.6
                    ,.."
                    "'0.5 Cl  04 r-03 0.2 0.1 0
g        ...g c
0 ll 0
Page 78of94
 
Statistical Analyses La rv:JI Fish Growth an d Survi val Tc st -7 Day Growth Stan Dale :      5/212017              Te~ I  ID* Ppl(CtCR                            S;omplA tn
* RFl'&deg;- RAr Tn~ic:;,nt End Oa1;i*        5/9/2017              Lab I~ ETS-Envir. Testing Sot                  Sample Typ .            KCL -Potasslum chloride Sample Date                              Pro1Dco1 FWCHR -EPA-821-R-02-013 Test Spec1 s                          PP-Pimephales promelas Comments:
Co n c-m IL                      2          3            4 0-Control        0.7530    0 .7060    07630        0. 6830 300    0.6100    0.5920    0.6090      0.7290 450    0.6590    0 .5860    0.6070      0. 54'50 600    0.5870    0 6450    0 6040      0.6760 750    0.4840    0-4800    04220        0. 5250 900    0.4700    0.4350    0.3110      0.3620 1050 0.0730        0.0000    0 0810      0 0000 Trans form : Untransformed                                1-Tafled                    Is otonic:
Conc* m !l'L      Mean      N-Mean      Moa n        tJlin        Max    CV%            N      t-Stat      Cri tical    MSD      Mea n      N-lllean 0 -Control      0.7288    1.0000    0.7288      0.6830      0 7630      5 578        4                                          0.7288      l .0000
                *300    0.6348    0 .8710    0.6348      0.5920      0.7290      9.980        4        2 .718      2.290      0,0792    0.634 8    0.8710
                *450    0.5990    0 8220    0 5990      0.5440      0.6590      7 .982      4        3.752        2.290    0.0792    0.6135    0.8419
                *soc    0.6280    0.8617    0.6280      0.5870      0 6760      6.403        4        2 913      2.290      0.0792    0 .6135    08419 750    0.4778    0.655      0.4778      0.4220      0.5250      6.868        4                                          0 4778    06556 900    0.3945    0.5413    0.3945      0.3110      0.4700    18.143        4                                          0.3945    0 5413 1050    0.0385    0 .0528    0.0385      0.0000 0.0810 1 15.781                4                                          0.0385    0 0528 A uxillal"t'. Tests                                                                    Statistic                Critica l              S k.e w      Ku rt Shapiro-Wilk's Teslindicates normal distribution (p > 0.01)                            0.92374                  0.844                0.70843    -0.4102 Bartlett's Test indicate$ egual variances(!!= 0.66)                                    0.76291                  11 .3449 H~ poth esis Test (1*Uil , 0.05)          NOEC        LOE C        ChV        TU      MS Du      MSD p        MSB        MSE    F-Prob          df Ounnett's Test                            <300            00                          0.07919 0 .10867 0.01266 0.00239 0 0148                    3 12 Treatmen ts vs D-Control Lin crar lntcrpolotl on (ZOO Rc::;amplc:; )
Point                mgIL      SD        95% CL(Ex p )          Skew 1co5*                116 29      61 .66    54 .14    436.34      1 8870 1c10*                232.58      85.50    108.28      6D2.01      1.6404 IC15                  408.09    138.74    126.89 754 .60            0 3100 IC20                  633.70      58.72    316.33      686.56 -3.8530 0 .9 IC25                  673.96      19.94    613.55      747.38      0.2701 IC40                  822.97      41 .45  7 15 37      958.28      0 3965                    0 .8 ICSO                  912.69      18.66 829 43          945.96
* 1 0693                        07
* indicates IC estimate le$$ than the lowest conc.en tration
                                                                                            =: 0 .6 c:
                                                                                            .g_
                                                                                            ~ 04 0.5 OJ 0.2 01 500          1000        1500 Do se mg/L Dose-Res ponse Plot 0.9 0.8 07                                                                          1 -!all, 0.05 level i  0.6 o f s gnlllcance e o.5 Cl
:04 0
                      ... 0.3 01 0 I 0
ec 0
0 6
Page 79 of94
 
              ~l *.-t-i ~ .P          .,
Page 5 of6
                                        .*
1 Species: Pim eplzales promelas                                                                                          PpKCICR Test Number: 25 Daily C!temist1y:
Day (Analvsl identified for cnch day, pcrfom1ed pH , D.O. and conductivity measurements onlv.)
0                                            I                                              2 Ana lyst                                      t-                  .,;...                                                                    ~
Co nccn tmtion        Pa r ameter 1l1
:  .;- '.  ~-
                                                                                                                              -*                  IL-Q *
                                                                                                                                                              .
                                                                                                                                                                ..        -.
                                                                                                                                                                              "'""      .- .- -                      *~        :-:~
                                                                          ,_
                                                                                              -                  *"  *..    *- -                              _.__ ~
: l.                                    - ~-*~"
pH(S .U.)                    g,2.\                ".}Pt~                      e>.10            "t.':\Z.                    e.oe            -+:~3 DO (mg/L)                      't.!1              .'.J.. .to                  +..:r              /l. Y                        *+.~                  '~.2..
Conducfi\'ity
                                                                                                                                                                                                                  .          ..;
(11111hos/cm)                  32..\
                                                                                                          .      "*      -32.6                            ..*              '3 t<i'      I*
                                                                                                                                                                                                    '
                                                                                                                                                                                            -            ; ..      - _>        -*
                                                                                                                                                                                                                      ,,,*...
                                                                                                              -                                      r..,: *;;:                                            .
                                                                                                                                                                          ~-~
                                                                                                                                                                                                              ._~I,..
C O NTRO L        "Alkalinity                                                                                                                                                                          '
                                                                                                                                            -
(m~ CaCOJIL)
                                              " H:irdnc s
                                                                                ~\            , .. ~-:~ <v
                                                                                                      - '.'[ ,,.
                                                                                                                              '4~
                                                                                                                                                -- --        -*
                                                                                                                                                            . .. .  ~
                                                                                                                                                                      ;':
                                                                                                                                                                                            . . *:                .. - .,.
(mg CaC01/L)                      8~                                            ~'?..                                            ~        i~
                                                                                                                                                                                                    .              ,,_. -
                                              *Temperature (0 C)            ,_'\."'\              "\S. '1.                ~.,                ,.,1..\. \                  1."'\.l.o            "'\.&.\ . ~
p H (S.U.)                    0.'1*                  ".l.e>j                ~.n"l-              1-\40                      So,p              '\.-s:1 DO (mg/L)                      ~-i:\                  f).._\O                  "+.'l              ~. \.(                      1-..d                ".\A JOO mg KC l/L      Cnn<l nrtivity                                                          l
                                                                                                                                                                .
                                                                                                                                                                                              ~
                                                                                                                                                                                              .                    "'* ,  ..'  ~
(um hos/cm)                  ~2                                            eq0                                          (  ett \                                    ..    -
                                              *Te m pera t ure (0 C)        "1.'\.i...          'L..S."\                -u>.,.\                "l.'-\*S                  "1..o.\.~            \.."\*'\
pH (S.U.}                      e.\'5                -1.. ~                    &O<P                ~.\12                      0()5                  '.} *" ' l 450 mg KCl/L DO (mg/L)                        '}.'\                  'l .ta                "".}.~              '+.3                          /l.A              ~.'?i. -
Conducli"itY i&deg;'\O                          '                                                                10"10
                                                                                                                          \070                                                                                                  *i
(&#xb5;mhos/cm)
                                            " Tcmpernture (&deg;C)              "l.'\.<o
                                                                                                      ~
                                                                                                  "'l-..o
                                                                                                            *~
                                                                                                                          "'\...\ .a              '\_'-\ . ~                L."'\.lo
                                                                                                                                                                                              *..r_ ...! ...:~
                                                                                                                                                                                                "'\)\ .            ,_
pH (S.U.)                      0 .\S                  1._~.:;-              e,.05              ~.t,O,                      AD~                    ~. \I                l D O (mg/L)                        e.o                    '.}lb                1\-a                  ~.t,                        "\!1                -:\..s .
600 mg KCl/L        Conductivity
(~1mhus/c111) 13'10                      .
                                                                                                              .
                                                                                                                '    -      low                                    :* l320                  .. ..
0
                                                                                                                                                                    -                    ' .        -***'
                                                                                                                                                                                                'bl.,_
                                            *Te m pera t u r e (&deg;C)        (.~lo                  '1..."'.* (-            "\.'\- &          L."\ *5                      t.'-\.1 p H (S .U.}                      'O.\~                1-.e4                    e.o5              ~.1~n                      e.02              '+.\10 D O (mg/L)                        eo            .      'l-.~
                                                                                                  ..                        8.o                "\;3
                                                                                                                                                                  .;
Q.Q
                                                                                                                                                                                          ..        ~3
                                                                                                                                                                                                    --        ...
750 mg KCl/L        Cond11c1ivity
(~tmhos/cm) 161o                      .                  lSLIO                                            16&.J
                                            "'Tcmpernture (0 C)          1,"1,.. lc.                'Lt;.o              "'\.~ . J          .,_"' * '-                    i."\*1            \.U.-S pH (5 .U.)                      e.\~                't.02.                    ~.o'1            1-.l61.                      e.03              '+.5t1 DO (mg/L)                      Q.0                    ~."l-                      t3J:J          /l. 3                          ~.o                  .}.3 900 mg KCUL                                                                                                    1.,.                                                                  -. - .
Contl11c1i\*ity
(~tmhos/cm) 1e3o                          ...    . le20 - . ..                                      le>\O                  ~
                                                                                                                                                                                                      ..._..... .
                                                                                                                                                                                                                ..:;.:.~*
                                                                                                                                                                                                                              ...
                                                                                                                                                                                                                            .,..
                                            " Temperntu r e (0 C)        1..L\. lo              1..~.o                    "l...\ . ~          -i'-\.'4                  "'\."'\ . ~            ,,~.s p H (S .U.}                    g.,,?>                ').C'~                0.o~              'l ti~                        e&+                '+.Sf-1050 mg KCl/L D O (mg/L)                        A.I                +.u                      ~.o                1-. ~
                                                                                                                                                    -.
6.o          . "'.}.  - .. ';"(
                                                                                                                                                                                                          ~
                                                                                                                                                                                                                      . . . 1l Conducli\'il)'                                                      ..                            ,                                                                                ;
(&#xb5;rnhos/cm}                    ~1:50                        .                ~100
                                                                                                                                                    ~ -
                                                                                                                                                              .    . ~\20                -.                            -
                                            *Temperature (&deg;C)              1.1.\."1                "\...*-\.'\            '"l.-!-'\          1..1.\ *..,                  1.c.\. ~            1."\. lo Initia l              Final                  Initial              F inal                    Initial                Final
  .......
ltn
* Temperatures performed :ll the time oftest initiation, renewal or termination by the analyst identified in the Doily Rcnew~l lnfonnnlion table loc.-ited on Page I.
w iw
              *nlin ity nnd hardnCEs performed by the ana lyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:                                                    t .    \
Page 80of94                                                                                                          SOP AT21 - Exhi bit AT21.I , revision 11-01-14
 
0 o
r I_,_] [_* -l ir.Q  l Page 6 of6 r* I 1-:      '          :--
Species: Pimeplzales promelas                                                                                                                    PpKCICR Test Number: 25 Day
{Analyst identified for each day. performed pH, 0 .0 . and conductivity measurements only.)
3                                                4                                                        5                                                      6 Ana lyst      ~                                  Pf:..t , ......
(<.J  IA-                  l.l.__            v---                                l)V                              jJ                    ,)v Concentr ation        Parameter                    ,,,..
                                                                      ~
                                                                      .;. . _,..;..;
                                                                                                  -- - * *~  IP
                                                                                                                          -  "
1~
I            ~-
7'        --      .r. ,
                                                                                                                                                                      - - ,.:;;;_*_...,_
                                                                                                                                                                                                          ;!'-.                  r* *.
                                                                                                                                                                                                                                ,_    .*    .        ;*~
pH (S.U.)
Pu"                          -:?.Cf~                    ?,.oi            ').~"'}                    0 .\'5                        "t.e2                              e.~                'J.w
                                                            "":l~
                                                                                                                                                                                                                                                                ..
DO (mg/L)                                                ~- 1                  -i. q                  ~q                        ':\.:}                      ':l-lP
                                                                                                                                                                                                      .. *. ..,                          ":).,A        ~. o Conductivity bZ.U
                                                                                                                                          -                                                                                  -
                                                                                                                                                                                                                                    ~20
:                -- .:J
                                                                                                                                                                                                                                                                          -~
                                                                                                                                                      -.
                              - (lam hos/cm) 311-              -      ' . --*              32'-{                                  __.. . .,. * .~.*~                        .        : ~
                                                                                                                                                                                                        --                                                          --. *-
                                                                                                                                                                                                                                                          .
                                                                                                                                                                          ..
                                                                                      *-    _    -r.';r_
                                                                                                      .                              ..
                                                                                                                                                                                                                      --- _. !~"'
* Alkalinity CONTROL (mg CaCO,!L)        :~f'-    ~
                                                                                                                    ~~                        "...  "        ~                                      ......                                      :          .~ -      *;~
                                                                                  .          ~---                                                                                        -                    ~
                                                                                                                                                                                                                                                                          ..
                                                                                        -
                                                                                                                                                                                          .
                                                                                                                                                                                                                      -
                                                                                                                                                                                                                                        ~
                                                                                                                                            -*                    v-
* Hardness                                                                                                        .~;;:
g(o
                                                          '
                                                                                                                                  ;
(m!!. CaCOJIL)                                            ..                                        -    .,;_                                                                    ;
                                *Temperature (oC)                  ""l!\. \o                        "\...\..-.\                "1.A. <a        '""\."\- lo                ..~ . f                    "\...~-"\                            !>\. &                  t.-l.* ~
pH (S .U.)              M1-                            1.'15                      ~ - \!              ')..53                    0 .\0                        ~-1'll                            ~    ,r:;:        ')-,\.( ~
DO (mgfL)                ~~                                ?-.'1                J *<6                  '\.0                        .-:\..~                    I'}.~                              /l..tl              14. \
300 mg KCl/L        Conductivity
                                                                                                                                                            ,                                    ..
                                                                                                                                                                                                    -
                                                                                                                                                                                                                                                                          >.
(;t mhos/cm)            <0'4 \                                                  '6'tt4                    **~      -        0&1                                                              0I05
                                *Temperature                                                                                                                                                '"1.'\.\o                          "\.-{ . '\
c*q                    \."\... r                      "\.4 .\a                  C..'l* 1            '\."\* ~                "\..'-'*'                                                                              "\.'\-1...
pH(S.U.)                611v.1                          1.":.11.1              <( . \2-              'l, Q"                    ~ .c:A                      'llA~                              0..\~                  'i-*62-DO (mgfL)                  ".\~                          +.r                    -:J A                  ~. o                      r:.\-.i-                        'l.ra                              "'.\-.~          (t.3 Conductivity                                        '
450 mg I CllL
{&#xb5;mhos/can)              l011)                                                  hC.O                                          h'iO                                                              \HO                      -
                                *Temperature
                                                          ""\.'\, .lo                  '\.'\ .1                  '""L'-\* \
l,.'{.\                      "'-"l*1                  \...'-\ *L                          "l!V\                "1.'\*)..
c*ci pH (S.U.)              0-05                          "J .'1-'l                <6. \ '2.-              ~-~                    8.dl                        'lM (JI                              ~ . \~              'l...sY DO (mgfL)                  ~ 1"\                        f. q                    S ..J                  (Oft                      l.l.~                        ').ca                              ".). t\            ~. 2>
600 mg KCl/L        Condncli\'ity
                                                        \320
                                                                                                            '                                    i
                                                                                                                                                        ' .
{11mhos/cm)                                                                \    l~O                                    --      a'-( 2.0                                              - , l3qo                                          -
                                *Tempernture L_-.\. b                      1.1\ * ~                  '1..'-l-1            '"\..'-\* (.o            l.~-1                          L.'-\..1....                      L.c.\.'\                  '1.'\.- S
('C) pH (S.U .)              e.os                            -:J.'1a                                      ':l-.50                    e .~                        '+-v~                              6.,\~
.I        750 mg KCl/L DO (mg/L)
Conducti\*ity lS9J e,o                            -:)._"
                                                                                                -
                                                                                                                '"fl.\'
i .o                  ~.,o                      ' t-d l~':lO
                                                                                                                                                                                              .,~*5"              -
                                                                                                                                                                                                                        -
00
(~2.0
                                                                                                                                                                                                                                                      ~
                                                                                                                                                                                                                                                        'l,l./G\
                                                                                                                                                                                                                                                      \(1.
                                                                                                                                                                                                                                                              ..-
3
(&#xb5;mhos/cm)                                                                      \~~                                                                                    ~' ~                                                          .'
I                            " Temperature
("C) pH (S .U.)
l.t.\ ':"'\
0.o~
C.~*"\
1-'l l
                                                                                                                  '"\."l-1
                                                                                                                ~ . I\
                                                                                                                                        ~-~
                                                                                                                                    ~-5!;
                                                                                                                                                                '"cl.1 e.oe t.."\.S
                                                                                                                                                                                            /)..tJef L."\ .'\
0.,\2..
                                                                                                                                                                                                                                                          )..'-\.~
                                                                                                                                                                                                                                                      "\.. ..,~
DO (mg/L)                  8,l                          :} .-:J..                                      I.DB                        q _(3                        'l-5"                              8.o                ~. t 900 mg KCl/L        Conductivity 4i * "                  ,.
                                                                                                                                                          ~
                                                                                                                                                                                                          -..        *
(&#xb5;111hus/c111)            ' 't?bQ                            ..
* l.-o\D                ,._.                        f0a'}o                              _.  .:.,          -    ""'    h3~0                          -    .* !
                                *Tcmpernture
                                                            'l..' i
* l                ).'\ .'\                                        '\."\.""                "2..' \-1                      \...'-\* 1..                        ~ .,                l-4..~
(&deg;C)                                                                              t."\. f pH (S.U.)                e .o~                          T--:&#xb5;;                    ~ .'09                ~ .vo                  A . o~                      'l..'1-'\                              0 .\2.          11..UP>
DO (mgfL)                    B.I                          +.-+-                      j ,'2.-              4 .~
                                                                                                                                            .  - - ..          ' ~. e;j                      r.}.u                              'b.l            l.O.?.
1050 mg KCl/L        Conduclivity (1lmhos/cm)              Z \lcO                                                2-\G:lf)                              ~          Al'-f 0                        .,          ....                  ~'YO
                                                                                                                                                                                                                                                                          '
                                                                                                                                                                                                                                                                          <
                              *Temperature
(&deg;Cl
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Initial                          Fina l                Initial                Final                  Initia l                      Fi nal                              Initial              Final
**  .,,::::~\:' pcraturcs performed al Liie time oftest Initiation , renewal or 1erminmion by the urrnlyst idt:11ti1icd i11 the Daily Renewal Information table located on Page I.
        ~lka inily and hardness performed by the analyst identified on tile bench sheet specific for each analysis and transcribed to this bench sheet by: _                                                                                            .
Page 81 of 94                                                                                                                            SOP AT21 - Exhibit AT2 I. I, revision l l-01-14
 
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Envlronmcmt411 Tostlng Solution s,, In c.
Source: In-house Culture Control Limits (+/- 2 Standard Deviations) 1.08
                              ------...
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                                                                                  *
                                                                                                                                                                                                                            -                                  -......._._.
                                                                    *                                                                              *
* 1.06                                        *                                      * *                                                              *                                                                  * * *
                            * *                                                                                                            *
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* t t  . . . o t. .. . t I o "O                                                                                                                                Warning Limits I
  "          1.10 1.05
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                                                                                                                                                                                                        * -**-        * ** -* *-* *- **-****                  .                  ~
1.00
                              .. .  . . . . . .. .  . . ... . . . . . . . ..... ,  .  . . . . . . . . . .... . . . . . . . . . . . . . . . . ...... . .  .. . .. .. .  ... .  .... . . . . . .. - *  * *  " .. .  . .  .  . . .  . . . . . . . . . . . ..... . f . 4 . , . ..
Test date
* 7-day IC25 = 25% inhibition concentration . An estimation of the sodium chl oride concentration which would cause a 25% reduction in Ceriodaphnia reproduction (calculated using ToxCalc).
Central Tendency (mean logarithmic IC25 oonverted to anti-logarithmic values)
Control Limits (mean logarithmic IC25 +/- 2 standard deviations converted to anti-logarithmic va lues)
-      ..- . Laboratory Warning Limits (mean logarithmic IC 25 +/- 2 coefficent of variations converted to anti-logarithmic values)
*.**. *.. . USEPA Warning Limits (mean logarithmic IC25 +/- 5A.io converted to anti-logarithmic valu es, s A.io = 10th  percentile of CVs reported nationally by USE PA)
Page 82of94
 
Ceriodaphnia dubia CQ
  ~
(I)                                                                                                    Chronic Reference Toxicant Control Chart W
co*  f: 1wo :mnwnc.at Ttt' ""SI Sol1,1 11Df'l,-, Inc,                                                                Source: In-house Culture
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Log 10 Conversion                                                            Anti-logarit hmic Values (g/l NaCl)
Test number                    Test date                      7-d ay IC25                                                                                                                      Laboratory Calculated CV      10th Percentile CV ToxCal Detem1lna tlon      7-day IC 25          CT                s                  CT                Control Limits                  Warning Limits            Wa rning Li m its (g/l N<iCIJ                                                                                      CT
* 2S        CT + 2S        CT - 2CV        CT + 2CV  CT . SA.10       

Revision as of 02:12, 30 October 2019

Submittal of Discharge Monitoring Report for May 2017
ML17163A414
Person / Time
Site: Sequoyah  Tennessee Valley Authority icon.png
Issue date: 06/08/2017
From: Anthony Williams
Tennessee Valley Authority
To:
Document Control Desk, Office of Nuclear Reactor Regulation
References
TN0026450
Download: ML17163A414 (101)
v • d • e


Text

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 8, 2017 Chattanooga Environmental Field Office Division of Water Pollution Control 1301 Riverfront Parkway , #206 Chattanooga, Tennessee 37402-2013 TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR May 2017 Enclosed is the May 2017 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period . The final report for toxicity testing conducted at outfall 101 , April 30 - May 5, 2017, is enclosed . No adverse effects were observed . If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714 . I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. ony L. W illiams Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosu res) : U.S. Nuclear Regulatory Commission Attn : Document Control Desk Washington , DC 20555

PERMITTEE NAME/AD DRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved . Name _ _!VA~EQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 0 1) Addres.L _f. ~BO X.1QOO _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ilNTEROFFICE OPS-5N-SQ!!l _ _ _ _ _ _ _ _ TN0026450 101 G F - FINAL _ _ _ __§ODDY - DAISY.._I.N_273B4_ _ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER D IFFUSER DISCHARGE FacJ!!ly_ _IVA-_fil:QUOYAH NUCL EAR PLANT_ _ _ _ _ _ 

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                                                                                                                                                             ~ice Anthony L. W illi ams                 personnel properly galher and evaluate the information submilled. Based on my inquiry o the person or persons who manage the system, or those persons directly responsible for gal ering P re:de nt lhe information, the informalion submilled is , to the best of my knowledge and belief, true,                                                       423         843-7001       17      06       07 S ite V ice President                 accurate, and complele. I am aware that there are significant penalties for submitling false          SIGNATURE OF PRINCIPAL EXECUTIVE                      I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.                 OFFIC ER OR AUTHO RIZED AGENT              AREA CO DE I    NUMBER       YE AR     MO      DAY COMMENTS AND EXPLANATIO N OF ANY VIO LATIONS (Reference all attachments here)

No c losed m o de operation . T he following injecti ons o ccu rred : Spectrus CT 1300 (max calc. was 0.039 mg/ml, limit 0.05 mg/L) , Spec t rus BO 1500 (max ca lc. was 0.046 m gfml, limit - 2 .0 mg/ml) . EPA Form 3320-1 (REV 3199) Previous editions may be used Pf!OP. 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved . Name_~VA~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres_L _£. ~BOX..£900 - - - - - - - - - - - - _ _ _ .J!NTEROFFICE OPS-5N-SQNl _ _ _ _ _ _ _ _ TN0026450 101 T F - FINAL _ _ _ _§ODDY - DAISYJ.N_..]7381._ _ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Fac_ill!y_ ..JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ LocatiO.!L _!!AMIL TOJi.COUNTY._ _ _ _ _ _ _ _ _ _ _ EFFLUENT DAY 31 NO DISCHARGE D ... ATIN :Millicent Garland NOTE: Read instructions before completini:i th is form. 

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared un~ _/ TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified --....,,__ Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry the person or persons who manage the system, or those persons directly responsible for g haring 1te Vice President the information, the information submitted is . to the best of my knowledge and belief, tru~ . 423 843-7001 17 06 07 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINC IPA L EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled April 30 - May 5 , 2017 . EPA Form 3320-1 (REV 3/99) Previous editions may be used Pa!'.le 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/L ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved . MAJOR Name _ _!VA~EQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.L_ ...£. ~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ .J!.NTEROFFICE OPS-5 N-SQ.t:l.l _ _ _ _ _ _ _ _ TN0026450 103 G F - FINAL _ _ _ _§ODDY - DAISY.._lN_]7381_ _ _ _ _ _ _ _ _ LOW VOL. WASTE TREATMENT POND Facl!l!Y_ _lYA - SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Location_ _!jAMIL TOJi.COUNTY..... _ _ _ _ _ _ _ _ _ _ EFFLUENT NO DISCHARGE D ... ATIN :M illicent Garland NOTE: Read instructions before completinQ th is form . IX PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH SAMPLE MEASUREMENT 

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PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Form Approved . Name _ _!VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR} OMB No. 2040-0004 (SUBR 01) Addres_L ..f.. ~BOX 20QQ... _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ .J.[NTEROFFICE OPS-5N-S(llil _ _ _ _ _ _ _ _ TN0026450 110 G F - FINAL _ _ _ _.§.ODDY - DAISYJN_1738L_ _ _ _ _ _ _ _ _ RECYCLED COOLING WATER Fac.illJY_ .JYA - SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l_ _!!AMIL TOJi..COUNTy_ _ _ _ _ _ _ _ _ _ _ EFFLUENT 

                                                                                                                                                                             ... NO DISCHARGE           Ixx I ...

ATIN :Millicent Garland x NOTE: Read instructions before completini:i this form . PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. CENTIGRADE SAMPLE MEASUREMENT 

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                                                                                     '"' with a syslem designed to assure that qualified accordance                                                                     /                                      TELEPHONE                       DATE Anthony  L. Williams             personnel properly gather and evaluate the information submitted. Based on my inquiry the person or persons who manage lhe system, or those persons directly responsible for g thering the information, the information submitted is , to the best of my knowledge and belief, Ir          ~e             ice President 423        843-7001         17       06      07 Site Vice President              accurate, and complete. I am aware that there are significant penalties for submitting false         SIGNATURE OF PRINCIPAL EXECUTIVE                 I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.                OFFICER OR AUTHORIZED AGENT            AREA CODE I   NUMBER        YEAR        MO     DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                  (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Paqe 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved . MAJOR Name_~~-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01 ) AddresL __E. ~BOX.1QOO _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _J!NTEROFFICE OPS-5N-SQN) _ _ _ _ _ _ _ _ TN0026450 110 T F *FINAL _ _ _ _.§.ODDY - DAISYJN_j7384_ _ _ _ _ _ _ _ _ RECYCLED COOLING WATER PERMIT NUMBER DISCHARGE NUMBER Faclfily_ ...IVA -_fil:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ r~RING p~*~t Location_ _!:!AMILTOJiCOUNTL _ _ _ _ _ _ _ _ _ EFFLUENT ATIN :Millicent Garland From 1 v~~* I~~ : ~~= To Hf ~~ I°tf 1 NO DISCHARGE Ixx I ... NOTE: Read instructions before completinQ this form . IX PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** 

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                                                      !he information, !he information submilled is . lo !he bes! of my knowledge and belief, lru Site Vice President
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No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved . Name _ _!VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres_L _f. ~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ilNTEROFF ICE OPS-5N-SQNl _ _ _ _ _ _ _ _ TN0026450 118 G F - FINAL _ _ _ __§.ODD..Y_* DAISYJN~ 7 3Bi_ _ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER FacJilly_ _.IVA-_fil:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio..Q_ .J:!AMILTOli_COUNTL _ _ _ _ _ _ _ _ _ _ EFFLUENT DAY 

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ATTN :Millicent Garland 31 NOTE: Read instructions before completinQ this form . NO. FREQUEN CY SAMPLE IX PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT 

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                                                                                                                                                     ~P<e,;deot NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under                                                                     TE LEPHONE                   DATE direction or supervision in accordance with a system designed to assure that qualified Anthony L. W illiams               personnel properly gather and evaluate the information submitted. Based on my inqui of the person or persons who manage the system, or those persons directly responsible fot gathering                                               -

the information, the information submitted is . lo the best of my knowledge and belie true, 423 843-7001 17 06 07 Site V ice President accurate, and complete. I am aware that there are significant penalties for submittin lse I 

                                                                                                                                           -       i--s1GNATURE OF PRINC IPAL EXECUTIVE TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.                OFFICER OR AUTHORIZED AGENT          AREA CODE I   NUMBER        YEAR      MO      DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

During th is reporting pe riod , there has been no flow from t he Dredge Pond other than that resu lti ng from ra infall. No Discharge th is Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Paae 1 of 1

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: May 24, 2017

1. Facility I Discharger: Sequoyah Nuclear Plant I TVA
2. County I State: Hamilton I Tennessee
3. NPDES Permit#: TN0026450
4. Type of Facility: Nuclear-Fueled Electric Generating Plant
5. Design Flow (MGD): 1,579
6. Receiving Stream: Tennessee River CTRM 483 .6)
7. lQlO : 3,491
8. Outfall Tested: 101
9. Dates Sampled: April 30 - May 05, 2017
10. Average Flow on Days Sampled (MGD): 1834. 1834. 1817 11 . Pertinent Site Conditions: Production I operation data will be provided upon request.
12. Test Dates: May 02 - 09, 2017
13. Test Type: Short-term Chronic Definitive
14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested(%):

Pimephales promelas: UV treated Outfall 101: 10.7. 21.4, 42.8. 85.6. 100 UV treated Intake: 100 Ceriodaphnia dubia: Non-treated Outfall 101: 10.7. 21.4. 42.8. 85.6. 100 Non-treated Intake: I 00

16. Permit Limit Endpoint(%): Outfall ] 01 : IC2.2. = 42.8%
17. Test Results: Outfall 101 : Pimephales promelas: IC25. > 100%

Ceriodaphnia dubia: lC.25. > 100% Page 1of94

18. Facility Contact: Millicent Garland Phone#: (423) 843-6714
19. Consulting I Testing Lab: Environmental Testing Solutions, Inc.
20. Lab Contact: Jim Sumner Phone#: (828) 350-9364
21. TVA Contact: Donald W. Snodgrass Phone#: (256) 386-2787
22. Notes: Exposures to samples collected April 30 - May 05, 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Page 2of94

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall l 01, Intake
2. Sample Type: Composite
3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (oC) (mg/L) Last Used Bv 04-30-17 0805 to 05-02-17 0920 101 05-01-17 1332 4.l , 2.2t <0.10 05-01-17 0705 05-03-17 0846 04-30-17 0710 to 05-02-17 0920 Intake 05-01-17 1332 1.7 <0.10 05-01-17 0610 05-03 -17 0846 05-02-17 0705 to 05-04-17 0850 101 05-03-17 1235 2.1 , l.7t <0.10 05-03-17 0605 05-05-17 0852 05-02-17 0730 to 05-04-17 0850 Intake 05-03-17 1235 2.2 <0.10 05-03-17 0630 05-05-17 0852 05-06-17 0930 05-04-17 0730 to 101 05-05-17 1230 1.1 , l.3 t <0.10 05-07-17 0952 05-05-17 0630 05-08-17 0833 05-06-17 0930 05-04-17 0745 to Intake 05-05-17 1230 0.9 <0.10 05-07-17 0952 

               . 05-05-17 0645 05-08-17 0833
 *TRC = Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- l .0°C) in a warm water bath.

Aliquots of Outfall l 0 l and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Page 3of94

Pimephales promelas Ceriodaphnia dubia Test Organisms:

1. Source: In-house Cultures In-house Cultures
2. Age: < 24-hours old < 24-hours old Test Method Summary:
l. Test Conditions: Static, Renewal Static, Renewal
2. Test Duration: Until at least 60% of control females have 3 broods
3. Control I Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
4. Number of Replicates : 1
5. Organisms per Replicate: lQ l
6. Test Initiation: (Date/Time): 05-02-17 0832 ET 05-02-17 0920 ET
7. Test Termination: (Date/Time): 05-09-17 0857 ET 05-09-17 0827 ET
8. Test Temperature: Outfall 101: Mean = 24.7°C Mean = 24.9°C (24.2 - 25 .1°C) (24.6 - 25.2°C)
9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each I 00% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).

Page 4of94

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted May 02 - 09, 2017 using effluent from Outfall 101 . Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control , 100 100 100 100 100 100 100 UV-treated 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Control , 100 100 100 100 100 100 98 Non-treated Mean Dry Weight (mg) Test Solutions (replicate number) (%Effluent) 1 2 3 4 Mean Control , 0.632 0.723 0.732 0.703 0.698 UV-treated 10.7% 0.700 0.704 0.701 0.677 0.696 21.4% 0.730 0.701 0.689 0.771 0.723 42.8% 0.687 0.642 0.662 0.684 0.669 85.6% 0.633 0.719 0.657 0.666 0.669 100.0% 0.644 0.649 0.627 0.637 0.639 Intake 0.650 0.605 0.596 0.688 0.635 Control, 0.632 0.783 0.630 0.669 0.679 Non-treated IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA 

     *TUa = 100/LCso: TUc = 100/ IC2s Page 5of94

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted May 02 - 09, 2017 using effluent from Outfall 101. Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85 .6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 . JOO Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 33 28 30 32 35 30 32 30 32 31.2 10.7% 34 36 31 34 33 31 32 35 31 31 32.8 21.4% 37 36 31 33 32 34 31 33 32 37 33.6 42 .8% 35 36 37 32 33 33 35 34 36 33 34.4 85.6% 37 35 34 38 32 38 38 36 39 32 35.9 100.0% 38 36 37 36 37 39 39 35 38 33 36.8 IC25 Value: > 100% Calculated TU Estimates : < 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA 

    *TUa = 100/LC 5o: TUc = 100/ IC2s Page 6of94

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted May 02 - 09, 2017 using water from Intake Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effiuent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effiuent) 1 2 3 4 5 6 7 8 9 10 Mean Control 33 31 32 34 34 31 28 32 30 31 31.6 Intake 38 34 36 37 35 31 37 32 34 32 34.6 IC2s Value: > I 00% Calculated TU Estimates: < 1.0 TUc* Permit Limit: NI A Permit Limit: NI A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA 

        *TUa = 100/LC 5o: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC2s) Pimephales promelas May 02 - 09, 2017 0820 7-days KCI 0.67 g/L Ceriodaphnia dubia May 02 - 09, 2017 0911 7-days NaCl 1.07 g/L Page 7 of94 

~    PBYSICALJCHEMICAL

SUMMARY

CQ Water Chemistry Mean Values and Ranges fi>r UV-treated Pimephales promelas and Non-treated Ceriodaphnia dubia, Sequoyah Nuclear Plant (SQN), Efiluent Outfiill 10 l and Intake CD perfi>nned May 02-09, 2017. ClO 

....

0 Test Sample ID Temperature ("C) Dissolved Oxn:en (llll?fL) pH~S.U.) Conductance Alkalinity Hanlness *Total Residual 

~                                        Initial       Final          Initial           Final           Initial         Final    (µmhos/cm) (mg/L CaC03) (mg/L CaC03)         Chlorine (mg/L)

Control, 24.7 24.5 7.8 7.0 8.13 7.69 322 62 85 - Non-treated 24.6 - 24.8 24.3 - 24.8 7.7 - 7.9 6.0 - 7.8 8.08 - 8.21 7.49 - 7.92 317 - 328 61 - 63 82 - 88 - - Control, 24.7 24.4 8.0 7.1 8.09 7.72 320 62 87 - UV-treated 24.6 - 24.8 24.2 - 24.7 7.9 - 8.2 6.2 - 7.7 8.05 - 8.19 7.55 - 7.93 312 - 326 61 - 64 86 - 88 - - 24.8 24.6 8.0 7.1 8.11 7.67 307 - - - 

            ~              10.7%

24.7 - 24.9 24.4 - 24.9 7.9 - 8. 1 6.2 - 7.7 8.06 - 8.18 7.49 - 7.89 295 - 318 - - - - - - 

              ~

i: 21.4% 24.9 24.5 8.0 7.0 8.11 7.67 294 - - - 

              <>..                    24.7 - 25.0 24.3   -   24.7 7.9     -  8.2    6. 1 - 7.7       8.04 - 8. 17   7.48 - 7.87 283   -  305   -        -   -      -     -                     -
            ~ "'                          24.9          24.6            8.0              6.9             8.09           7.67        259            -            -                       -
              "'
            --=            42.8%

24.8 - 25.0 24.2 - 24.9 7.9 - 8.1 6.0 - 7.7 7.98 - 8.18 7.49 - 7.87 250 - 267 - - - - - - 

            -~

Q., 85.6% 25.0 24.6 8.0 6.9 8.09 7.67 190 - - - 24.9 - 25.1 24.2 - 24.9 7.9 - 8.1 6.0 - 7.7 7.99 - 8.19 7.49 - 7.86 186 - 193 - - - - - - 25 .0 24.6 8.0 6.9 8.08 7.65 165 64 65 < 0. 10 100% 24.9 - 25.1 24.2 - 24.8 7.9 - 8. 1 5.9 - 7.7 8.03 - 8.19 7.48 - 7.87 160 - 169 59 - 69 64 - 66 < 0.10 - < 0. 10 25.0 24.6 8.0 7.0 8.09 7.69 165 64 67 < 0.10 Intake 24.9 - 25.l 24.4 - 24.9 7.9 - 8.1 5.9 - 7.7 8.03 - 8.18 7.48 - 7.91 158 - 170 61 - 67 64 - 70 < 0.10 - < 0.10 Control, 24.7 25.0 7.8 7.9 8. 13 8.03 322 62 85 - Non-treated 24.6 - 24.8 24.9 - 25.2 7.7 - 7.9 7.8 - 8.0 8.08 - 8.21 7.97 - 8.09 317 - 328 61 - 63 82 - 88 - - 10.7% 24.8 25.0 7.8 7.9 8.12 8.02 308 - - - 

            .s 24.7 - 24.9 24.7 - 25.2 7.8         -  7.9    7.7   -   8.0    8.06 - 8.18    7.97 - 8.09 298   -  316   -        -   -      -     -                    -
            ..,,                          24.8          25.0            7.8              7.9             8.13           8.01        292            -            -                       -
            .§             21.4%
            .s=:

24.7 - 24.9 24.8 - 25.2 7.8 - 8.0 7.8 - 8.0 8.09 - 8.19 7.97 - 8.08 286 - 300 - - - - - - 

            --=            42.8%

24.8 24.9 7.9 7 .9 8.12 8.02 258 - - - 

            .§'                       24.7 - 24.9 24.8 - 25.0 7.8         -  8.0    7.8 - 8.0        8.08 - 8.19    7.97 - 8.09 248   -  265   -        -   -      -     -                    -
            *~             85.6%

24.9 24.9 7.9 8.0 8.12 8.02 188 - - - 

            ~

24.7 - 25.0 24.8 - 25.1 7.8 - 8.0 7.9 - 8.1 8.08 - 8.19 7.97 - 8. 10 182 - 196 - - - - - - 24.9 24.9 8.0 8.0 8.08 8.01 164 63 65 <0.10 100% 24.7 - 25.0 24.8 - 25.2 7.9 - 8.0 7.9 - 8.2 7.95 - 8.19 7.92 - 8.09 158 - 171 59 - 67 62 - 66 < 0.10 - < 0. 10 24.9 24.9 8.0 8.0 8.09 8.04 166 64 66 < 0 .10 Intake 24.7 - 25.0 24.8 - 25.0 7.9 - 8.1 7.8 - 8.2 8.02 - 8.18 7.98 - 8.11 162 - 177 59 - 67 64 - 68 < 0.10 - < 0. 10 

     *Note : Total residual chlorine was perfonned on non-treated Outfall 101 and Intake samples.

Overall temperature ("C) Average Minimum Maximum Pimephales prome/as 24.7 24.2 25.l Ceriodaphnia dubia 24.9 24.6 25.2

SUMMARY

I CONCLUSIONS Exposures to samples collected April 30- May 05 , 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Page 9of94

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction oftest organisms. UV treatment is used to control interference of fish pathogens. This treatment method was approved on November 23 , 2015 by the State of Tennessee in a letter from Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring.
2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS I. Pimephales promelas None
2. Ceriodaphnia dubia None Page 10of94

PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable) .

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
3. Temperature was measured by SM 2550 B-2010.
4. Dissolved oxygen was measured by SM 4500-0 G-20 I 1.
5. The pH was measured by SM 4500-H+ B-2011.
6. Conductance was measured by SM 2510 B-2011.
7. Alkalinity was measured by SM 2320 B-2011.
8. Total hardness was measured by SM 2340 C-2011.
9. Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC2s values in g/L KCI or NaCl.
2. Standard Toxicant: Potassium Chloride (KC! crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.
4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 11of94

REFERENCES l . NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-82l-R-02-013 (October 2002).
3. Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012.
4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 12of94

Sequoyah Nuclear Plant Biomonitoring May 02 - 09, 2017 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 13of94

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 2017 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat TRC EO/PO 02/06/2005 - <0.0042 0.028 0.010 - - - - - 02/07/2005 - <0.0116 0.028 0.010 - - - 0.007 - 02/08/2005 - <0.0080 0.028 0.010 - - - - - 02/09/2005 - 0.0199 0.028 0.010 - - - - - 02/10/2005 - <0.0042 0.028 0.010 - - - - - 02/1112005 - 0.0155 0.028 0.010 - - - 0.007 - 0610512005 - 0.0063 - - - - - - - 0610612005 - 0.0043 - - - - - - 0.037 0610712005 - 0.0103 - - - - - - 0.037 06/08/2005 - 0.0295 - - - - - - 0.037 0610912005 - 0.0129 - - - - - - - 06/ 10/2005 - 0.0184 - - - - - - - 07117/2005 - 0.0109 0.026 0.009 - - - - - 07/ 18/2005 - 0.0150 0.026 0.009 - - - - 0.036 0711912005 - 0.0163 0.026 0.009 - - - - 0.036 07/20/2005 - 0.0209 0.026 0.009 - - - 0.014 0.036 07/21/2005 - 0.0242 0.026 0.009 - - - - - 07/22/2005 - 0.0238 0.054 0.018 - - - 0.014 - I 0/30/2005 - 0.0068 - - - - - - - 10/3112005 - 0.0112 - - - - - - - 11 /01/2005 - 0.0104 - - - - - - 0.035 11 /02/2005 - 0.0104 - - - - - - 0.036 11 /03/2005 - 0.0117 - - - - - - 0.036 11 /04/2005 - 0.0165 - - - - - - 0.035 11/14/2005 - 0.0274 - - - - - - - 11115/2005 - 0.0256 - - - - - - - 11/16/2005 - 0.0234 - - - - - - - 11117/2005 - 0.0231 - - - - - - - 11/18/2005 - 0.0200 - - - - - - - 11119/2005 - 0.0116 - - - - - - - Page 14of94

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 2017 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L 101 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC EO/PO Phosphate 11 / 12/2006 - 0.0055 - - - - - - - - 11 / 13/2006 - 0.0068 - - - - - - 0.037 - 11/14/2006 - 0.0143 - - - - - - 0.037 - 11/15/2006 - 0.0068 - - - - - - 0.037 - 11/16/2006 - 0.0267 - - - - - - 0.037 - 11/17/2006 - 0.0222 - - - - - - - - 11 /26/2006 - 0.0188 - - - - - - - - 11/27/2006 - 0.0138 - - - - - - - - 11/28/2006 - 0.0120 - - - - - - - - 11129/2006 - 0.0288 - - - - - - - - 11 /30/2006 - 0.0376 - - - - - - - - 12/01 /2006 - 0.0187 - - - - - - - - 05/28/07 - - - - - - - - - 0.015 05129107 - - - - - - - - 0.036 0.015 05 /30/07 - 0.0084 - - - - - 0.017 0.036 0.015 05/31 /07 - 0.0103 - - - - - - 0.036 0.015 06101 107 - 0.0164 - - - - - 0.017 0.036 0.015 06102107 - 0.0305 - - - - - - - 0.015 12/02/07 - 0.0241 - - - - - - - - 12/03/07 - 0.0128 - - - - - - - - 12/04/07 - 0.0238 - - - - - - - - 12/05/07 - 0.0158 - - - - - - - - 12/06/07 - 0.0162 - - - - - - - - 12/07/07 - 0.0175 - - - - - - - 04/ 13/08 - 0.0039 - - - - - - - - 04/ 14/08 - 0.0124 - - - - - - - - 04/15/08 - 0.0229 - - - - - - - - 04/16/08 - 0.0143 - - - - - - - - 04117108 - 0.0120 - - - - - - - - 04/ 18/08 - 0.0149 - - - - - - - - I 0/26/08 - 0.0260 - - - - - - - - 10/27/08 - 0.0151 - - - - - 0.017 - - 10/28/08 - 0.0172 - - - - - - 0.041 - 10/29/08 - 0.0154 - - - - - 0.018 0.041 0.030 I 0/30/08 - - - - - - - - 0.041 0.030 10/31 /08 - 0.0086 - - - - - - 0.041 0.030 Page 15 of 94

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Micro biologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 2017 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg!L Quat mg!L TRC Phosph EO/PO Quat Phosphate ate 02/08/09 - 0.0197 - - - - - 0.017 - - - 02/09/09 - 0.0237 - - - - - 0.017 - - - 02/ 10/09 - 0.0104 - - - - - 0.021 - - - 02/1 1/09 - 0.0155 - - - - - 0.017 - - - 0211 2/09 - 0.0106 - - - - - 0.017 - - - 02/1 3/09 - - - - - - - - - - - 05110109 - 0.0129 - - - - - - - - - 05111109 - 0.0415 - - - - - - - 0.0446 - 05/ 12/09 - 0.0053 - - - - - - - 0.0396 - 0511 3109 - 0.0049 - - - - - - - 0.0396 - 0511 4109 - <0.0141 - - - - - - - 0.0397 - 0511 5109 - <0.0160 - - - - - - - - - 11 115109 - 0.025 - - - - - - - - - 11 116109 - 0.0152 - - - - - - - - - 11 /1 7/09 - 0.0255 - - - - - - - - - 11 /1 8/09 - 0.0306 - - - - - - - - - 11 /1 9/09 - 0.0204 - - - - - - - - 11120109 - 0.0093 - - - - - - - - - 0510911 0 - 0.0192 - - - - - - - - - 05/ 10/ 10 - 0.0055 - - - - - - - - - 0511 111 0 - 0.0100 - - - - - - 0.039 - - 05/ 12/1 0 - 0.0171 - - - - - - 0.039 - - 05/ 13/1 0 - 0.0041 - - - - - 0.039 - - 05/ 14/1 0 - 0.0099 - - - - - - 0.039 - - Page 16of94

Table B-1. Sequoyah Nuclear Plant Outfall l 01 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 2017 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cupro stat H-130M Nalco Spectrus H-150M MSW Fioguard Hypo- mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 MS623 6 chlorite TRC mg/L Copoiy- DMAD Azoi e Quat mg/L mg/L Quat mg/L mg/L mg/L Phos- mer EO/PO Quat Phos- Phosphate TRC oh ate oh ate 10/31/ IO - - - - - - - - - - - - I I /0 1/1 0 - 0.01 22 - - - - - - - - - - 11/02/ 10 - 0.011 2 - - - - - - - - - - 11 /03/1 0 - 0.01 63 - - - - - - - - - - I 1/0411 0 - 0.0107 - - - - - - - - - - 11/05/ 10 - 0.01 32 - - - - - - - - - - 05/01 /2011 - - - - - - - - - - - - 05/02/2011 - - - - - - - - 0.04 - - - 05103120 I I - - - - - - - - 0.04 - - - 05/04/2011 - 0.0 155 - - - - - - 0.04 - - - 0510512011 - 0.0179 - - - - - - 0.04 - - - 05/06/2011 - 0.0089 - - - - - - - - - - l l /06/201 I - 0.0168 - - - - - - - - - - 11 /07/2011 - 0.0225 - - - - - - - - - - 11 /08/2011 - 0.01 41 - - - - - - - - - - 11 /09/2011 - 0.0239 - - - - - - - - - - 11/] 0/2011 - 0.0242 - - - - - - - - - - 11 /J 1/2011 - 0.0231 - - - - - - - - - - 05/06/201 2 - - - - - - - - - - - - 05/07/201 2 - - - - - - - - - - - - 05/08/201 2 - - - - - - - - 0.041 - - - 05/09/201 2 - 0.0 145 - - - - - - 0.041 - - - 05/10/201 2 - 0.0298 - - - - - - 0.041 - - - 05/J 1/201 2 - 0.0174 - - - - - - - - - - 08/J 2/201 2 - - - - - - - - - - - 0.029 08/13 /201 2 - 0.0256 - - - - - 0.028 0.037 - - 0.029 08/ 14/201 2 - 0.0209 - - - - - - 0.037 - - 0.029 08/J 5/201 2 - 0.0279 - - - - - 0.028 - - - 0.029 08/J 6/201 2 - 0.0076 - - - - - - - - - 0.029 0811 7/201 2 - 0.0446 - - - - - - - - - 0.032 05/ 12/20 13 - 0.0099 - - - - - - - - - - 051131201 3 - - - - - - - - - - 0.064 05/1 4/201 3 - 0.0091 - - - - - 0.039 - - - 0.064 05115/201 3 - 0.0096 - - - - - 0.03 9 - - - 0.064 05/1 6/201 3 - 0.0229 - - - - - - - - - 0.032 05/ 17/201 3 - 0.0063 - - - - - - - - - 0.032 09/ 15/201 3 - - - - - - - - - - - 0.03 0911 6/20 I 3 - 0.0072 - - - - - - 0.0379 - - 0.03 09/1 7/201 3 - 0.0 107 - - - - - 0.036 0.0379 - - 0.03 09/ 18/201 3 - 0.0217 - - - - - 0.036 0.0379 - - 0.03 09/ 19/201 3 - 0.0 172 - - - - - - - - - 0.03 09/20/201 3 - 0.01 73 - - - - - - - - - 0.03 Page 17of94

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxic ity Test Sampling, February 6, 2005 - May 5, 201 7 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cupro stat H-130M Nalco Spectrus H-150M MSW Floguard Hypo- mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CTl300 mg/L 101 MS6236 chlorite TRC mg/L Co poly- DMAD Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos- mer EO/PO Quat Phos- Phosphate TRC phate phate 05/04/2014 - 0.0118 - - - - - - - - - - 05/05/20 14 - 0.0112 - - - - - - - - - - 05/06/2014 - 0.0096 - - - - - - - - - - 05/07/2014 - 0.0164 - - - - - - - - - - 05/08/20 14 - 0.0235 - - - - - - - - - - 05/09/20 14 - 0.0110 - - - - - - - - - - 09/07/20 14 - - - - - - - - - - - - 09/08/2014 - - - - - - - - 0.04 - - - 09/09/2014 - - - - - - - - 0.04 - - - 0911 0/2014 - - - - - - - - 0.04 - - - 09/ 11 /2014 - 0.0070 - - - - - - - - - - 091121201 4 - 0.0074 - - - - - - - - - - 08/09/20 15 - - - 08/J 0/2015 0.0195 0.03 - 08/1 1/2015 0.0275 0.03 - 08/1 2/20 15 0.0213 - 0.03 08/ 13/201 5 0.0192 - 0.03 08/\ 4/20 15 0.0182 - 0.03 I 0/ 18/2015 0.0162 I 0/1 9/20 15 0.0125 10/20/2015 0.0120 10/2 1/201 5 0.0130 10/22/201 5 0.0174 10/23/20 15 0.0156 0511 5/2016 - 0511 6120 16 0.0209 05/1 7/20 16 0.0210 05/18/2016 0.0361 05/1 9/2016 0.0254 05/20/2016 0.0261 07131/2016 - - 08/0 1/20 16 0.0091 0.03 08/02/20 16 0.0093 0.03 08/03/201 6 0.0209 0.03 08/04/20 16 - - 08/05/20 16 - - 04/30/20 17 - 05/01 /20 17 0.0298 05/02/20 17 0.0218 05/03/2017 0.0260 05/04/20 17 - 05/05/20 17 - Page 18of94

Sequoyah Nuclear Plant Biomonitoring May 02 - 09, 2017 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 19of94

BIOMONITOIUNG CHAIN O F CUSTODY RECORD Page~l~of~l~ 

 ~ ent:                                                                                                                                                               Delivered By (Circle One):

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' oject Name: Sequoyah NP Toxicity Environmental Testing Solution, Inc.

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Instructions: Clients should fill in all areas except those in the "Laboratory Use'* block. Biomonitoring samples are preserved by storing them al 6°C and shipping them in ice. The hold time for each sample is 36 hou rs from the time of collection. Therefore. please collecl and ship in such a way that the laboratory wil receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Whole Effluent Sample Receipt Log Page 52 

                                                                                       *sample temperature performed using Sample Receivitlll Thermometer : SN 160928622 Date    Ti me   Received    Received
  • sample Project Sample 0

Sample name and description State Comments Received Received by from Temp. ( C) number number 05-01-17 1245 J. Sumner l. Keenan 4.1 12330 170501 .01 Jefferson WWTP NC I 05-01-17 1245 I. Sum.,er l. Keenan 4.1 12331 170501 .02 West Jefferson WWTP NC I 05-01-17 1332 J. Sumner TVA Courier 4.1/2.2 12332 170501 .03 TVA - Sequoyah Nuclear Plant - 101 TN I 05-01-17 1332 J. Sumner TVA Courier 1.7 12332 170501 .04 TVA - Sequoyah Nuclear Plant - Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12

BIOMONITORING CHAIN OF CUSTODY RECORD Page _l _ of _1_ Delivered By (Circle One): 

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 'fioject Name: Sequoyah NP Toxic ity Environmental Testing Solution, Inc.

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  ~* Number: N/ A                                                                                            Asheville, NC                                             Other (specify):   52.. . , ,*c_ Del<<uer'y General Comments('
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Instructions: Clients should fill in all areas except those in the *'Laboratory Use** block. Biomonitoring samples arc preserved by storing them at 6°C and shipping them in ice. TI1e hold time for each sample is 36 hours from the time of collection. Therefore. please collect and ship in *uch a way that the laboratory will recei ve the samples with ample lime to initiate testing within that time frame. Samples shipped overn ight on Friday via FedEx or UPS musr. be marked for Saturday delivery or they will not arrive until the following Monday.

Whole Effluent Sample Receipt Log Page~ 

                                                                                             *sample temperature performed using Sample Receiving Thermom eter: SN 160928622 Date      Time    Received     Received     *sample    Project   Sample Sample name and description                 State             Comments 0

Received Received by from Temp. ( C) number number 05-03-17 0910 K. Keenan Fed - Ex 0.7 12346 170503 .01 Rockingham WWTP NC 05-03-17 0950 K. Keenan Fed - Ex 1.0 12347 170503 .02 ApexWRF NC 05-03-17 0950 K. Keenan Fed - Ex 1.2 12348 170503 .03 Belews Creek SS NC 05-03-17 0950 K. Keenan Fed

  • Ex 4.1 12349 170503 .04 Daikin Applied Americas NC 05-03-17 0950 K. Keenan Fed - Ex 0.3 12350 170503 .OS Dan River CC NC 05-03-17 0950 K. Keenan Fed - Ex 2.4 12351 170503 .06 Davidson Water WTP NC 05-03-17 0950 K. Keenan Fed
  • Ex 0.8 12352 170503 .07 Dobson WTP NC 05-03-17 0950 K. Keenan Fed
  • Ex 1.2 12353 170503 .08 James Loughlin WWTP NC 05-03-17 0950 K. Keenan Fed
  • Ex 0.8 12354 170503 .09 Performance Fibers NC 05-03-17 0950 K. Keenan Fed
  • Ex 1.2 12355 170503 .1 0 Roseboro WWTP NC 05-03-17 0950 K. Keenan Fed - Ex 0.3 12356 170503 . I I Scarlett Acres MHP NC 05-03-17 0950 K. Keenan fed - Ex 0.8 12357 170503 . 12 Sparks Road WTP NC 05-03-17 0950 K. Keenan Fed
  • Ex 2.0 12358 170503 .13 Elementis NC 05-03-1/ 0948 K.Keenan UPS 10.5 1 2359 170503 .14 Morehead City WWTP NC Exceede:J Temp. requirement 05-03-17 0950 K. Keenan Fed - Ex 1.7 12360 170503 .15 AQWA NC 05-03* 17 0950 K. Keenan Fed - Ex 1.7 12361 170503 .16 Bladen Bluffs WTP NC 05-03-17 0950 K. Keenan Fed - Ex 1.7 12362 170503 .17 Buckeye Terminals NC 05-03-17 0950 K. Keenan Fed - Ex 1.2 12363 170503 . 18 lnvisita
  • 001 NC 05-03-17 0950 K. Keenan Fed - Ex 1.2 12364 170503 . 19 lnvisita
  • 002 NC 05-03-17 0950 K. Keenan Fed
  • Ex 0.1 12365 170503 .20 ManteoWWTP NC 05-03-17 0950 K. Keenan Fed - Ex 0.3 12366 170503 .21 Mayo Stream Electric Plant NC 05 17 0950 K. Keenan Fed - Ex 1.2 12367 170503 .22 Pender County WTP NC 05-03-17 0950 K. Keenan Fed
  • Ex 1.3 12368 170503 .23 Shearon Harris Plant NC 05*03-17 0950 K. Keenan Fed - Ex l .5 12369 170503 .24 Shearon Harris E&E Center NC 05 17 095Q K.Keenan Fed - Ex 1.0 12370 170503 .25 Washington WWTP NC 05-03-17 0950 K. Keenan Fed* Ex 1.2 12371 170503 .26 PCS Phosphate, Inc. NC 05-03-17 0950 K. Keenan Fed* Ex 0.3 12372 170503 .27 Woodlake Yacht Club NC OS-03-17 0950 K. Keenan Fed - Ex 0.3 12373 170503 .28 TVA
  • Allen Fossil Plant - 001 TN 05* 03-17 0950 K. Keenan Fed
  • Ex 0.2 12373 170503 .29 TVA
  • Allen Fossil Plant - Intake TN 05-03-17 0950 K. Keenan Fed* Ex 0.1 12373 170503 .30 TVA - Allen Fossil Plant - Upstream TN 05-03-17 0950 K. Keenan Fed - Ex 1.2 12374 170503 .31 Carolina Beach WWTP NC 05-03-17 1152 J. Sumner Dash Courier 0.3 12375 170503 .32 OWASA
  • Mason Farms WWT-:> NC 05-03-17 1215 K. Keenan L. Keenan 4.0 12330 170503 .33 Jefferson WWTP NC 05-03-17 1215 K. Kee nan L. Keenan 4.0 12331 170503 .34 West Jefferson WWTP NC 05-03-17 1235 I. Sumner TVA Courier 2.1/1.7 12332 170503 .35 TVA
  • Sequoyah Nuclear Plant- 101 TN 05-03-17 1235 J. Sumner TVA Courier 2.2 12332 170503 .36 TVA - Sequoyah Nuclear Plant* Intake TN SOP G4
  • Exh ibit G4.2, revision 01-03 -12
 .

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.le~~ 6:om dtc time Of ~lc~on. TI{eteRln. plea. c:Oll~ and khip In ~~Ira Wf/i <<mt t114 J.iio~ory- will receive tllC ~ with amplt rime to1TI1hiµc t~ whhln that. time fra111e* .$1 mpl~ sb1ppe,d . ovt;trughl Qn .Ftlday 'i'ia FlldBx.'Qr UMJ must be RU!tkad fbr 68turdll.Y .dettvtry or~ WlfI not 1ltl'fVC unall the following Mond'ay. 

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Whole Effluent Sample Receipt log Page Sta 

                                                                                          'Sample 1emperature performed uslne Sample Receiving Thermome1er: SN 1609286~2 Date     Time    Received   Received     *Sample   Project   Sample Sample name and description                  State             Comments Received   Received     by        from     Temp. (°C) number   number 05-05-17    0943    K. Keenan   Fed - Ex       0.9     12347  170505 .01   Apex WRF                                                NC 05 17   0943    K. Keenan   fed* Ex        0. 1    12348  170505 .02   Belews Creek SS                                         NC 05 17   0943    K.Keenan    Fed - Ex       3.3     12349  170505 .03   Daikin Applied Americas                                 NC 05-05-17    0943    K. Keenan   Fed* Ex        0.8     12350  170505 .04   Dan River CC                                            NC 05-05*17    0943    K. Keenan   Fed - Ex       2.1     12351  170505 .05   Davidson Water WTP                                      NC 05-05-17    0943    K.Keenan    Fed - Ex       0.3     12352  170505 .06   DobsonWTP                                               NC 05-05-17    0943    K. Keenan   Fed - Ex       0.9     12353  170505 .07   James Loughlin WWTP                                     NC 05-05-17    0943    K.Keenan    Fed - Ex       0.3     12354  170505 .08   Performance Fibers                                      NC 05-05* 17   0943    K. Keenan   Fed - Ex       0.4     12355  170505 .09   Roseboro WWTP                                           NC 05*05-17     0943   K. Keenan   Fed - Ex       0.2     12356  170505 . 10  Scarlett Acres MHP                                      NC 05-05-17     0943   K. Keenan   Fed - Ex       0.3     12357  170505 . 11  Spa(ks Road WTP                                         NC 05-05-17     0943   K. Keenan   Fed - Ex       0.7     12358  170505 . 12  Elementis                                               NC 05-05-17     1220   K. Keenan Dash Courier     0.4     12375  170505 .13   OWASA- Mason Farms WWTP                                 NC 0 5-0!>-17   1220   K. Keenan Dash Courier     0.7     12346  170505 . 14  Rockingham WWTP                                         NC 05-05-17     1240   K. Keenan  J. Brewer       1.4     12330  170505 . 15  Jefferson WWTP                                          NC 05-05-17     1240   K. Keenan  J, Brewer       2.4     12331  17050S  .16  West Jefferson WWTP                                      NC 05-05-17     1230   J. Sumner TVA Courier    1.1/1.3   12332  17050S  . 17 TVA - Sequoyah Nuclear Plant - 101                      TN 05-05-17     1230   J. Sumner TVA Courier      0.9     12332  170505  .18  TVA - Sequoyah Nuclear Plant - Intake                   TN SOP G4 - Exhibit G4 .2, revision 01-03-12

-, I Page I of 7 l Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeplzales promelas Client: Tennessee Vallev Autboritv County: Hamilton Facility: Seguovah Nuclear Plant Outfall: 101 NPDES # : TN0026450 Project #: Di/rdio11 prepnratio11 illformntio11: Commellts: Dilution prep (%} 10. 7 2 1.4 42.8 85.6 100 Each concentration was UV-treated Effiucnt volume (mL) 267.5 :i35 1070 2140 2500 for 2 minutes to remove pathogenic Diluer,1volume (mL) 2232.5 196; 1430 360 0 Interferences. To1al volume (mL) 2500 2500 2500 2500 2500 Test organism illformatioll: Test ill{ormation: Organism source: In-house culture Randomizing template: PM.e. '\eu..D\a) Age: < 24-hours old Incubator number and 

                                                                                                                                                     \(..

shelf location: Spawn date: o"\* 1.."\* \""\ Arte111ia CHM number: CHM9 14 Hatch dates and times: C~

  • C\* \\. \\00 'lJ Dry ing information f or weight OS*~i.*\""'\ o~o'S determillntiou:

Transfer vessel infom1ation : pH - \.~I., s.u. Date I Time in oven: l'l!t*CA"l-, M.~n Temperature == c.'.\..'\ "C Initial oven temperature : l.i'\. (. Average transfer volume: < 0.25 mL Date I Time out of oven: !f\C::. ... D*l'"l nA-..n Fina l oven temperature: L..Q -c: Tota l drying time: ~~*llo\l/1.~ Daily feeding mu/ renewal iuformntiou: Dny Da te Morning feeding Afternoon feeding Test initiation, Sa mple num bers used MHSW renewal, or batch term ination used Time Analyst Time Analyst Time An alyst Ou tfall 10 1 In take 0 05 17 01..10 11. \"1..:io w 0~:!>1. ~ 

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l\ ri<.t\ \ * (C) '~ C>~o \. o-\ o"\< 'llo* n" I 2 05-03-1 7 05 17 0 "- l:>O 0'-0~ I 

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no.S~.~lo O~* l.l.*11~ o"-*i.s.*,,6 3 05 17 O\o\'I;) 1t{ \'1.lt:) l.I oSS"L ({' ,,os~.~s rt o.s o~. :a.i.. t)~*lb*&'li) 4 05-06-1 7 01\~ \-1 \~\<:. \,{ ~o ~ I"'\ OS.OS. \\ \\O~ . \R QS.*01.*\1 5 }{ o~ ,,osa;. \~ 

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OS'?)?) w nn~~. \1 no~.t8 nc;. *(ft.* 1, 7 05 17 04S-'\ >.\ Co11trol i11formatio11: Vl* C..c~oL Acceptance criteria Summmy of test endpoints: 

           % Mortality:                                          01.                        S20%                7-day LCso             >     1001.

Average weight per initia l larvae: o.<-,~~ NOEC 1007. Average we ight per surviving larvae: o.t..'\e ~ 0.25mg/larvac LOEC ~ lO() 7. ChV "'> lN\1. IC15 >1001. Page 26of94 SOP AT20 - Exhibit AT20.3, revision 11-01 -1 4

.: ,..--........_..._ Page 2 of 7 Species: Pimepltales promelas Client: TVA I Sequoyah Nuclear Plant. Outfall 101, UV-treated Date: 05-02-17 Survival and Growth Data Day CONTROL 10.7% 21.4% A B c D E F G H I J K L 0 10 10 IO 10 10 10 JO 10 10 JO 10 IO 

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IO IC> IO J {) l~ I() IC l t> to IQ co tO 2 

                                                      /\)       to       IO          10       10     lD        IQ         lO      lD     I CJ      10     f()

3 10 10 io 'D 10 lQ l () IQ '0 I() lO t() 4 ft) tD ID 'D 'D ID IQ ( (J I t) '0 l () 

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5 llJ 10 ID  !() (() I() I\:) ID ID 11) l(J co 6 ID ID ID It) 11;:} It> 'a l(J IQ I C 10 10 7 It> I l) l () I0 I() I0 /\) ID IO /0 t lJ IQ 

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A - Pnn we igh! ~ Troy co lor code:: >> L 0!0.XJrC.. \S.o3 \s.ec /to.~ llP. 03 (5.ot l "1.1... l~O!J lY.~ 13.~~ l5.'4S-Annlyst: iJ..., Dnl c: O"'*cii* 1:r l'S.~ ll = Pan + Larvae '[>'.;ht (mg) Analyst: J.1.00 AJ~ a.135 ~ .93 ~3.Sb ~3. 0-=l >>i~,o:l. ;o.q l ~'-*31.P ;1,l.4$ ~o.SlJ ;l3, I(,. Date: 01<5.) I .1): C = Larvae weig ht ( mg)= B - A Hnnd cnlcul nlcd. (.. ~'L 11. t. ~ "'\. ~l 1.<f:!> ~ . b~ ,.o'-l "\.O\ '-*,., "'\.oc ,.o\ lo. ~C\ ,,,\ Arrn lyst: 

                                   ~

Wei ght per initial number ofl arvnc {mg) 

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   = C / Initial number of lar-vn e                                                                     .)..
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Hand cnlculated. Ana lys t: 

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C>' Average wei ght per Percen t reduction o.~1. - ~.b 1. initiul numbcr of larvae (mg) rrom co ntrol (%) 0- "'°" &- - 

                                                                                 "             o.\o'\lo                            O.""\"l."b Comment codes: c = clear, d =dead, fg =fungus, k = killed, m = missing, sk =sick, sm = unusually small, lg = unusually large, d&r = decanted and returned. w = wou nded_

Comments: SOP AT20 - Exhibit AT20.:;, revis ion 11-01-14 Page 27 of94

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- , Page 3 of7 l Species: Pimeplwles promelas Client: TVA I Sequoyah Nuclear Plant. Outfall 101. UV-treated Date: 05-02-17 Survival and Growt!t Data Day 42.8 % 85.6% 100% M N 0 p Q R s T u v w x 0 10 10 10 10 10 10 10 10 10 10 JO 10 1 

                                                           /0        10         IC          10       ID       co           lQ       l {)       tO             10        10         IQ 2

fO IQ to t \) L{) l \) ta 10 { {) I\) '0 IC) 3 I0 ID 10 lO lO 10 I() lb lb ID 10 co 4 I0 ro l () ID /"{) [ 1) lD 10 LO 10 tO lb 5 

                                                           '{)       I (J     10          l \}      I iJ      ID        10         ID         10              10      IQ         10 6

I0 10 IQ I () /Q 10 I~ ID l'\j ID 10 ID 7

10 ID It:> 10 /{) lb CD 10 I() /0 tO *D A ,. Pa n weig ht (m£t Trny color cod e:: yciqoo*.s..e- \S~<

~?i.tt.3 ~\~ ~!.~ c:it0.11 ~1 .qa Date : 06. \\. f.}-- C = Larvae weigh t ( m g) ~ 13 -A ... ~1 (o.~1. t,,,\o1. ~.i~ ~.~ ,, \t:\ 1..s1 c.., lolo 1oA'-\ "'.'-\'\ ~."2.1 1.;.~1 Hnntl cnlcnl ntetl. Analys t: ti W eigh t per initia l number of la rl'ae (mg) = C / l nitia l number of la n *ae Hant! cnlculatctl. ~ "' tf, ~ ~ tV ..... -.g .)-. q. ~ ~ °' '-:1"' '° -.&) 'I>

t' ~

~ ~ 'I> IO \0 "' " i:5"° ~* ""11 "\O A nnlyst : ~ o* <). Q' 0 o* '\). o* Cf r;;;;,' c' Average weight pe r Pe rcent retlu clio11 in itia l number of from control (%) 0. IJ/'t '-\. \ 1. O* '4:-\.~ 1..\.\1. o.~:,'1 s.. '-\ 7. la r vnc (rn ~) J Comment codes: c - clear, d - dead, fg = fungus, k = kil1cd, m - missing, sk - sick, sm = unusually small, Jg= unusually large, d&r = decanted and returned. w = wounded. J Co111me11ts: Page 28of94 SOP AT20 - Exhibit AT20.3. revision 11-01-14 Page 4 of7 Species : Pimeplral es pr ome/as Client: TVA I Seguova h Nuclear P lant, Outfall 101. UV-treated Date: 05-02-17 S urvival mu/ Growth Data Day 100% In take Control - No n-t rea ted y z AA BB cc DD EE FF CG HH a JJ 0 IO JO 10 IO 10 10 10 10 I (0 10 I0 10 IQ to 10 ( () 2 to I \:J to co to to ID 10 3 ID fO I \) 10 10 10 I CJ IQ 4 lD ID ID ID l b /(j IQ IQ 5 l'O I {) ID 10 IQ IQ I (J to 6 IQ I D I I) 10 f(J J () tO IQ 1$Jo\ 7 I t:> <1'-t 10 10 JO {() "'.,c. f\) A= J>nn weight (mg) Tray color code:: i.j~ v cXU lSA4i l'-4. 143 }5,Lt4 llti.16 lS.e>o \5.82. \S.0'.P 15:-:k) Ana lyst: ,. !{., Date: 01.l .aJa!.

  • I ~

3 = Pan + Larvnc weight (mg) Analyst: ~.\.l lj /H>.IP8 a1.43 AA~J ~:;l.12 '"3 .lo5 ~.Iv ~. '3'l Date: ~.II*(.}- C = Larvnc weight (mg) = B - A (, .So t. .os ~ .'\'- (o, i& c,. ~"L "l. t.1J ~ .'!,0 .... Ir;.'\ Hnnd calculated. Ana lyst: ~ Weight per initial number of lan*ac (mg) = C /Initial number of larv:u: Hn nd calculntcd. c,,O 6-' ~ ~~ '11,.,,'V' a."'IJ ....,o .._,vtt-. Ana lyst: ~ "° '\:)' o*'° £.; o* o*"" o* " \:) o*""° o* ~ Average weight per Percent reduction ~Ot"" initial number of from control (%) &\. C)1. 0. (.,1t:\ to~S ~t>PL~l!Le lnrvne (mg) 0

  • Comment codes: c = clear, d
  • dead, fg = fungus, k = killed, m - missing, sk = sick, sm = unusually small, lg= unusually large, d&r =decanted and returned, w =wounded.

J Co111111e11ts: Page 29 of94 SOP AT20 - Exhibit AT20.3, revision 11-01-14 TVA I equoya h Nuclear Plant, Outfall 101 Ma 02-09 201 7 Pim ephflles pro111e/11s h ro nic Wh ole Ef'nuc111 Tox icity Tc *1 El' -82 1- R-02-0I J. i\lclhotl IUOU.O Qn:ililv *0111rol Veri ficati on of Data E ntry, Calc uluti on , 1111<1 ' 1:1tislicnl Annlyscs Envlronmllntal Testing Solutions, Inc. 12Jll N0t for Comph;inci: 1\ 1..~ nK*m . lim:m~ l.abonnory QC (\*n<('ftl r.14~ cV.1 Ac:pl"~'< 1nhbt 11* "*bt.t*r lann t1n1I IUtmhrr t1f bn llC " . 1'1ri .. irt;::llt (n11.t1 8

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TVA / equoyah Nuclear Plant, Outfall 101 Ma 02-09, 2017 Statistical Ana ly e Larval Fish Growth and Survival Tost-7 Day Growth Start Date: 51212017 Test ID: PpFRCR Samole ID: TVA I SO N Outfall 101 End Dale: 5/9/2017 Lab ID: ETS-Envir. Tesling Sol. Sample Type OMR-Discharge Monitonng Report Sample Date: May 2017 Protocol: FWCHR*EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comment.s: UV-Treated Conc ..0/o 1 2 3 4 Non-Conlrol 0.6320 0 .7830 0.6300 0 .6690 UV-Control 0 .6320 0 .7230 0.7320 0 .7030 10 7 0 .7000 0 7040 0.7010 0 .6770 Willie hypotnesfs test results indicate a slgnlflcant dlllerence n growth 21.4 0 .7300 0 7010 0 .6890 0 .7710 for the 100% OuUall 101 sample, tho PMSO w as below the lower bound 42.8 0 6870 0.6420 0.6620 0.6840 (12%) os1ablishod by EPA. Guidance In EPA 833 -R-00.003 (June 2000) 85.6 0 .6330 0 .7193 0.6570 0.6660 Soclfon 6.4. 2 for determining lhe NOEC was followed, and ii w as 100 0 6 440 0 .6490 0.6270 0.6370 concluded that lhe hypothesis lest yielded a Type I Error. lnlal<e 06500 0 .6050 0.5960 0.6880 T ransform : Untr;af1Sformed 1-Tallod Is otonic Conc */o 6 Moan N*Moan Mo.an Min Max CV°!. N I.St.at Crl tlcal MSC M ean N*Moan Non- Control 0.6785 0.0726 0.6785 0 .6300 0.7830 10.602 4 UV-Control 0.6975 1.0000 0.6975 0 .6320 0.7320 6.497 4 0 .7053 1 .0000 10.7 0 .6955 0 9971 0.6955 0.6770 0.7040 1 790 4 0 .094 2. 410 0.0511 0 .7053 1.0000 21 .4 0 7228 1.0362 o 7228 o 6690 0 .7710 5 046 4 -1 190 2.410 0.0511 0.7053 1.0000 42.8 0 .6688 o 9588 0 .6688 0.6420 0 .6870 3 145 4 1 355 2. 410 00511 0.6666 0 9483 85.6 0 .6688 o 9589 0 .6668 0.6330 0 .7193 5 445 4 1 352 2.410 0 .0511 0.6688 0 9483 -100 0 .6393 0 .9165 0 .6393 0.6270 0 .6490 1.492 4 2 .746 2.410 00511 0.6393 0 906* Intake 0 .8348 o 9 oo 0.6348 05960 0 .6880 6 .718 4 Auxili~~ Te sts St:>tlstic Critie~I Skow Kurt Shapiro-Wolk"s Test indicates normal di slritlulion (p > 0 .01) 0 .97487 0 .884 -0.2395 0.86461 Bartlett's Test ondocates equal v riances (p z 0 14) 8.38396 5.0863 The control means are not s19n10car'\UV ditleren1 <!! = 0 .67) 0 .<:<:695 2.44691 H~pothuls Test !1*1.all, 0 .05! NOEC LOEC ChV TU MS Du MS Op MSB MSE F-Prob df DunneU's Test 85.6 *100 92 .5203 1 16822 0 .05112 O.C*7329 0.00341 0 .0009 o 01612 5. 18 Treatments vs UV *Conlrot L ln &~r 1n1orpo1auo111200 R&sal!'lp 10 ~) Poi nt  % so S6% CL(E.>:E) Skew IC05 42 096 24 .500 17.808 122-928 0.4432 IC10 >100 IC15 > 100 1.0 IC20 >100 0 .9 IC25 >100 0 .8 IC40 >100 tCSO > 100 07 . 06 "' c: 0..5 .." 0 Q, 0: 04 03 0'2

0. 1 00

-0 1 0 50 100 150 Dose %~ Ooso-Respon so Plot 09 o.e 0.7 _::-.::-.7. 7- ~-"'---i:i--~~ Hall. 0.05 level * * * * *

  • __ . __ . _ . _. _____- _:-.§ ::-

... 06 of significance 'i e o.s " 0

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0 g co "'"'"' 0 0 ~ c c  ;;; ~ .!! u 0 0 0 s .: > z0  :> Page 31of94 TVA I equoya h Nuclear Plant Outfall 101 - Intake May 02-09, 2017 Statistic:il Ana lyses Envtro nm e "~' Tcs11n9 SoluUon1. Inc. L a rva l Fis h Growth a nd Surviv al Test-7 Day Growt h Start Date: 5/2/2017 Test ID: PpFRCR Sample ID: TVA I SQN O utfall 101 - Inta ke End Date: 5/9/2017 Lab ID: ETS-E,vir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2017 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales prome las Comments: UV-Treated Con e-% 1 2 3 4 Non-Control 0.5320 0 . 7830 0 .6300 0.6690 UV-Control 0 .5320 0 7230 0.7320 0 .7030 10.7 0 .7000 0 .7040 0 .7010 0 .6770 While hypothes is tes t results indicate a significant difference in growth fo r 21 .4 0.7300 0 .7010 0.6890 0.77 10 the intake sample, the PM SO was below the lower bound (12%} es tablished 42.8 0.6870 0 .6420 0.6620 0_6840 by EPA. Guidance in EPA 833-R-00-003 (June 2000) Se ction 6.4.2 fo r 85.6 0.6330 0 .7190 0 .6570 0 .6660 determining the NOEC was followed, and it was concluded that the 100 0 .6440 0 .6490 0.6270 0 .6370 hypothesis test yie lded a Ty pe I Error. Intake 0 .6500 0 .6050 0.5960 0 .6880 T ra n s fo rm : Untransformed 1-Tailed Con e-% Mean N-Mean Mea n M in M ax C V% N t-Stat Critical MSD Non-Control 0 .6785 0.9728 0.6785 0.6300 0 .7830 10.602 4 UV-Control 0 .6975 1.0000 0.6975 0.6320 0 .7320 6.497 4 10 .7 0 .6955 0 .997 1 0.6955 0.6770 0 .7040 1.790 4 21.4 0 .7228 1.0362 0.7228 0.6890 0.7710 5 048 4 42.8 0.6688 0.9588 0.6688 0.6420 0.6870 3.145 4 85.6 0.6668 0 .9588 0.6688 0.6330 0 .7190 5.425 4 100 0.6393 0.9165 0.6393 0.6270 0 .6490 1.492 4 *intake 0.6348 0 .9100 0.6348 0.5960 0.6880 6.718 4 2.017 1.943 0 .0605 A uxilia ry Tes t s S tati s tic Critical Skew Kurt Shapiro-Wilk's Tes: indicates normal distribution (P > 0 .01 ) 0.95314 0 .749 -0 .4316 -0.9929 F-Test indicates equal vanances (p = 0.92) 1. 12947 47.4683 The control means are not significantly different (p 0.67 ) = 0.44695 2 .44691 Hypot hes is Test (1 -ta il , 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0 .06046 0 .08668 0 .00788 0 .00194 0 .09029 1, 6 Treatments vs UV-Control Dose-Res pon se Pl ot 0.2 0.1 0 g ": "" 0 .l: c: 0 c: 0 0 ... "' 0 <.:; u c: 0 >

>

z Page 32of94 ) ,__ ....,...._~ Page 5 of7 Specie : Pimepftnles promelas Date: 05-02-17 Clien t: TVA I Scguovah Nuclear Plant, Outfall 101. UV-treated D at*1JI Ch emtstry: . Day (Analyst identified for each day, pcrfom1cd pH , D.O. and conductivity measurements only.) 0 I 2 Analyst y.- 1.J.- . I>-- ,........ .. -:::. . "'v Jv Con centration Parameter - f *~ ~ * - - . - -, -- *' .: ,. - ~.:: " ... - ..... *~ .......... - * .....c..o.: .. ~.,., _ ~s. -- ..- . ~ oH (S.U.) a. 1'\ 'l._q~ (b{}l.a ':\..u" e.ov "".},"""' DO (m!!.11.) Ila t;\-.f.O '+A (O.~ '~.a ~-2 Conductivity . ~ - *~ CONTROL (µmhos/cm) 322-i ':' I *;\' " ~ , .... .3Z6 ..~ ~ . .... 01C\ ..... *;*..- * ** *. ' 1........ __ * *...-. *

  • t '~ ' 1

*Alkalinity ~\ \p...\  :*%. :.**** .. '"'-...-N'"' . *~*~.: 'r.:. UV-treated (mg CaCO;/L) .. *:*'*'**- **-* *:.. .. ,~*.Ir- *- - .. -. -- ..,. ~* ~ *Hardness ' (me CaC03'L) &~ &l.o -=-* " *Temperature ("CJ "t~-"\ \.'-l * \o \..'\*lo ~\.-~ "1:-\..J ~**\."\.. pH(S.U.) c&.\6 ll.f.>'t e.4"\ ')._\JI\ eo~ ~.~ DO (mwL) 1\-.C\ ~.v . ~-q (O.~ ~.q '+.l 10.7% Condu ctivity fomhos/cm) ~OC\ ' ' .. "- 3l0 . '* . .' 3oto .:*., > " Tempera t ure ("CJ '1.'\.& 1.~A '"\.'-\*l '"\..'-l-1 "\.'-\. '\ "'\A.A pH (S.U.) e.1v t:\-.0~ ra-' o '.\.\tl A1~ ~.5"}- 'l.q {J).v .. DO (mwL} ':\ .~ ~-"' \O.'l ~-~ 21.4% - - Co nductivity ~ I> (J.lmhos/cm} ut\ 2>12. ' . ~; "' Temperature (°C} "\.'\. << ~-1 \."\* 1 \1-\.\ LS:o '"'-"'* '{ nH(S.U.J S.14 ~.)')- ~ . \O '+.\O\ ece ~ .....~ DO (m!?/Ll ~'1 I}.:::; '+q (/1 \ - 0.0 - in< 42.8% Conduclivity UPO .. 2e:::J.o I (~1mhos/cm) i.'\. .g .,_ ..t.1-~ - - "' Temperature C°C) \..'{.'\ "" ~\.S l..~*O ,.i{.I.. pH (S.U.) . ~\~ '\..t>S e>.\\ '.\.~ ao::\ ~.~ DO(m!?/Ll ~o 'l-.5 'tY lll'Z. 0.0 l4."1-85.6 Vo Conductiviry (~1mhos/cm} l~ ,q' 16<.o -- . - *Temperature (°C) 1-"\.~ t.*V1 "'\!-\. '\ 1.."\-1 '1.'S. 0 ,_~.c.. pH (S.U.} 0.\$" /}.PM e>.~s "+ ,;f 0.0'-l "\..5tP DO (mg/L) {Jj.J) ~.<I\" t:\.&I u,?_ 6.o <J1.eJ - .. .. . .;;. Conducti\'ily I l,/J::) (umhos/cm) .. --. .. *~s l~O -

  • Alkalinity

.c -.. - -...- ...... - -,'f~'~ . .. . j 100% S(\ .-  ! toS (mg CaC03'L) *H:t rdncss t,...\ - ... . ::.* -: . -  :.- .," - .-.. * -7~ . - ** :ii. *. ~ .. -*' vl.o ~ - .]ij ;~~ (mg CaC03'L) .... . -;- *:- ,, ., *TR chlorine (mg/L) < 0.10 '"' " . -......... - - r ..-:-* . . -<o .10

  • Temperature (°C) pH (S.U.)

"\..~. 0 6.11-' l."\ * ~ r1dl'< Pln~ . 1.'1 . '\ "L"\. cf ' ll1Al A03 "\."i. \ 'l."53 '"l.'{. '° DO (mg/L) ~-1 , .J..l.P ~ ./J l.l)."'\ A.O  !.#,~ Conductivity tll3 . .. . .~:t. . r ~ . t 146"' .  ;!~ ., -. 168 . - . ,'fl;.\, (umhos/cm) - . . . . .i...:; . * 100% lntal<c *Alkalinity (m2 CaCO;/L) (9\ . *; - ~r ... . .. .. .-:-* -~ . . .-* \c~ . J~,,

  • I I

~'1 *Hardness . lab (ma CaCO;/LJ - .,_,_ . ~ - ----- '* 1&.1' . - ~ *TR chlorine (mg/L) <o .tO  ; ~- <0.10 - *Tempcrnturc (°C) L.,"(.'i '\.-( .c\ 1.~-~ '1."\. s n. \ 1,\.(, 'i Initial Finn i Initial Final Lniti:t l Final ,......... mpcraturcs pcrforlllcd at the lime oftest initiation, renc' al or termination by the analysl identi fied in the Da ily Renewal Information table located on Page I. ...... wiR,lk inily, h dness and tot;il residual chlorine perfonned by th an alyst id~nti(i~cl on rhc bench ~heel ~pccilic for each anal y~ i ~ and trnn ~crihecl In 1hi~ hcnch ~heel ~__..,__. Total residual chlorine was performed on non-treated Outfall IOI and lnrakc samples. Page 33 of94 SOP AT20 - Exhibit AT20.3, revision 11-01-14 * - c--:::J lf=l ~ - I . Page 6 of 7 Species: Pimeplwles promelas Cl'1en t : TVA I Seauova h N uc Iear Pl an t . 0 u t[a JI IOI UV-trcate d . Date: 05 17 Day (Analyst identified fo r each day, performed pH, D.0 . and conductivity measuremen ts only. ) C oncen-trntion Paramete1* nH (S.U.) Analyst [[ . v-- - f?>.oS 3 . -. ~~ +.~l - ,, - ~.\O .,_4 ' '.}.§ "'-- - ,~- . I~ i B - IA- , 5 .. ')..A~ ~ _. .Iv

. - .-

6 J.f-..1 ~ -*- rJ.Sl- -* f'l,.. 0.\"' DO (ml!/Ll g .o 'J.~ c;c.. 2..- "'* -s 'lo~ 'l.<  !'.}Pf (jf,z... Cond uctivity 22.LP i r::t . -  ; 3\2- - . -*----*-- . 32\ -, - C111nhos/cm) - CONTROL - -- -' ... .r ~A" ~ *Al kalinity - ~.~"' '1~ UY-treated (mg CaCOJILl - - \cl1- ..--- .. ..."!_ _.!,._ ~ . "' . ' '* ~ *~ -.~ *llardncss "' &'4 .- I (ma CaCO;/L) - *Temperature (°C) ""\........ ~ \..-t*"- "'U\* ~ . '"\.. \. '\ \..--\* \ \.."'\. "'\ ~'-\-~ "\..4,.1-- riH (S.U.) A.i~,, '":f . ~4- g' *\ 2.- 'l,,o..\ t'\ l~ 'l.e'-t 0.\1.p ~.\('1 DO(m!!IL) (JL() 1..1- <l. \ lnVl e.o ~.5' .. ~.q ((), '2... 10.7% Condu cti vity ~. - I (jam hos/cm) 210 ~'ts- 3"1 3oS b-\.\ ""\.'\.<\ .......... \o "'\.~.'" ~-'"\ *Temoe*rnture (°C) '-'-t*S \.."'-*" "\.~*"" *** 1.JA pH (S.U.) e.o4 -J.-:n- ~ .\\ ".t*-5\P ~~ '.l..~3 ~.\-:\. DO (mlifl.) ~ .o -:J.r-+- <6.1~ to.+ . 6.J.J "".},"' Jl..tJ <.P \ 21.4% Conductivity .. ~ ,'.' *' ~q+ (umhos/cm) *Temperature (°C) ~otld -,s...Q "'\.'l

  • c; c!X~3

~-0 - '"\..'-\* \.:. I "'l_..\.~ \.~*~ - 2°t?, ~-0 W' '"\.-l*'-' riH (S.U.) ';}.°'S' ".1:*:n- ~.o'l 'l.o..l e.O'\ 'l.~i.t e.\e, T.}.,~q DO (m2'Ll 6..0 J..1- ~.\ I0.6. ,,. t!>.O ~.14 "),,q 14. 0 42.8% Conduc tivity ~ .... - I* ..... * {11111hos/cm) a1..1l ~~'° ~143 ... ' ~5(p *Temperature (°C) "'\S. 0 "'I .... la \S.o -..,'-\. b -U-\. ~ "'\A*S '"\.Ci. .o '-"\* ,_-*. 1>H (S.U.) '-l-A'l ""J..'1-r ~.\Q l'.\-.S"~ OIJ"'IL.4 ".\-.0(0 0,\G:\ '.}...~ 85.6% DO (mg/L) Corul uclivity 0.0 'J.'l- ({ . ' I0,1..D . .* .., es.1 ':).ta ~ /.}.q <P.o -- ll\) \Kl - l'13 lql ., (µmh os/cm) ~ Temperature C°C) l.S.. \ "\_'-t

  • le, - u.a L."\.1 l.'\.'\

~ L.!.\*l-. ~.o - ""\..'-\* "L pH (S.U.) f:')O?i.  ?."'=Iv "iLD 't ~-SV 01'11.11 '+.A".} e.\a .fl.:*I~ DO (mlifl.) e.o l.t- ~. l (a.~ e.o ':)~ 9-0 ~q Cond llctivity (µmhos/crn) \ lt>'":r 11.tS- . l~ ll.o~ .. ,_

.. - -* .-i..;. ~ - ~ 100%

  • AJknlinity - . -;; ' r

-~ - . -- - .. . (mg CaCO;/Ll *ll:irdncss - - ! - ""°' (.~ . - ' ~:l .~ ,* . (mg CaCO;/L) - ~ . - - - - *~ .' ~ ,_ - ~ -'.- ' - - *TR chlorin r (mg/L) *Tempera ture (°C) ~* - . '"\S. \ - "\!-\. s . I f"l.I(') -u;. \ .4}- ... -r-\*.S -- L.S.O ' "'t.L\*1- '* ~ \.~. \ '1..-t.~ DH (S.U.) ~DS -'J,,f'C( ~.'i::l"1- r~.lJ2. 0.0""} .tlC\\ {:). la '}.~0 DO (ml!/Ll so -i...v 'J..CJ f.o.~ .. . '6.o "l'l- -:JA ':).Cf - Conductivity 1 - - .' - \~1-  ; -- ~ (1m1hcs/cm) iuie . : l '4 \ -- -*- *- 11v ' 100% "Alk:ilinity -'*~ . ' . .. ' ; -- ~-* ,.-. - Intake (me CnCO;/L)  : ., J -.

-;i, ' <o\ _li

- . .: 'Ji. .. . - .. "- ~-...- ,, - - -. *Hardn ess f' ' . ~ (mg CaCO,;/Ll .,..-:1 "' *- 10 -  : ~: . . I* i' ~ I~: .. '31 - ,. . -* .. I* *TR clllorinc (mg/L) ., . LD*\0 - ./ . ~ ,,_-----: ~- *- ' - *Tempera tur e (°C) "l$..O l..J.-lo 1.S* \ 1,.'{ ., ,~:o 1..'-l* ~ 1$. \ 1..'"\.S Initial Final lnitial Final Initial Final Initial Final llUft n '** : wi 'tc 1peratt1res pcrfonned at the time of test initiation, renewal or tcm1ina11on by the analysl idcntilied m the Daily Renewal ln fo nnation table located on Page I. "-"*v* ..Alka 'nity, hardness and total residual chlorine performed by the analyst ident ified on lhe bench sheet specific for each analysis and l ra n~ cri bed to lhis bench sheet tf.J>y: . Total residual chlorine was perfo rmed on 11011-trcated Outfall IO I and lnlake samples. P e 34 of 94 SOP AT20 - Exh ibit AT20 .3, rev ision 11 -0 1-14 Page 7 of7 Species: Pimep ha les promclas Client: TVA I Sequoyah Nuclear Plant, O utfall 101, UV-treated Date: 05-02-1 7 D auy *1 Cl1e1111sfrJ1: Day (Ana lyst identified ror each day, nerfonncd pH, D.O. and conductivity meas\lrcmems onlv.) 0 l 2 Analyst if..; ..}--- iJ...,; IA 1-- . ,..)-. ,..., Concentration Parameter - ~ -- *-~ - ' " ~ .. *- .. ' *: ~ nH (S.U.) e .2\ 'J..c:Ji1- ~.IQ '\.ut4 a.oe, -t .-sz. Co ntrol DO (m!!IL) *+~ '.t.s *.;.7- r~.2.. +:1- ~ . I.cl No n-treated Conductivity (~u11hos/cm) ~2.l "l.'20 - - " ~- ,, 3lC\ -. - q~-;; ~- I '* - - - . . . . .' ... *Alkalinity "°4>&\ ~..r"" ' ~; \o L- .i (111~ CaCO;/L) *A,_ ~ - ~ - u. - - - ~ -- ~ *Hardness 'i 11112 CaCQ,/Ll -  :' .. t1- .~ 'j ~ ' -~ "Temperature (°C) ""l."\.\ ~'-\- < "\.<\. .., ""\:'\. s 1.."l .\ '1..'-l . lo Initia l Final Initial II Fina l Initia l II Final Day (Annlyst identified for each day, performed pH. D.O. and conductivity measurements only.) 3 4 5 6 Ana lyst v--- ~ (,L. ~ I).; w ti'- .µ Vv . "'"" 1,: .r .* . Co ncen-trntion Parameter r  !~ *. . .. - . * ~. - .

*' ~**

. -~ '. . 6 '

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*.r' nH (S.U.) 5.\~  :+."i2.. ~ ~ - . ~ °"~ t-$4 e .l-s- 'l.A..! PU°I '.l.4'\ -:i.'b IO*--\ "l.-:r '}.~ Control Non-treated DO (mg/L) Conductivity J...~ . *-~ - ~. 'l-.112 .. ;w G! o . (µ mhos/cm) s2v 3 rl . 32.4 .* ' " """ .- - ... , - ~' -.. ... .. "' l~o-"\ ~- ~ *Allrnlinity (mgCaCO:i/L) ~t.V-'""' - . -- '°::::, ' .., . ..,, I ~ ~ ' *Harclncss ~ (mg CaC01/L) - . 8b .  ; ~ .,_, *Temperature (°C) -,.,\. b ""\-'-\ \.'-\.lo ..~. "':> &..'-\. ') "'\..'-\. ~ ~ tl.!.'-1 Initial Fi nni In itial Finni ""'"'*\ Initial Fi nal Initial Fi nal *Temperatures pcrfom1ed nt the time oftest initiation. renewal or tenmnalton by the analyst 1dent1fied in the Dally Renewal 1nromiat1on fable located on Page I. Alkalinity, hardness and total residual chlorine pcrfonned by the onalyst identified on the bench sheet specific for each analysis and transcribed to th is bench sheet by: - *. Page 35 of94 SOP A T20 - Exhibit AT20.3 , rev ision 11-01-14 TVA I Seq uoyah Nuclear Plant, O utfa ll 101, UV-treated Ma 02-09, 2017 Pim epltale.~ prom eills Chronic W hole Efflu en t Tox icity Te t EPA-82 1-R-02-0 13, Method 1000.0 Da il y C hemica l A nalyses Envlronme.n tal T~srlng Solution)., Inc. Project numllcr" 1?332 ~~~~~~~- C oncc11tmtiu11 11nrumctrr On *O Dul' I Ou\ 2 1 Onv 3 D:tv 4 Da v s Dn v 6 lnitiu l Finul lnillnl Fimtl lnit lnl 17 in11I lnitinl Finni lnithtl Finni I nit In I final Initial Final !ull IS lJl 8.2 1 7.92 8. 10 7.66 8.08 7. 52 8. 13 7.83 8 .08 7 511 8. 15 7 84 8. 19 7.49 00 (mi!/Ll 7.9 7 s 7.7 7.2 7.7 6.6 7.7 7.8 7.8 6 .4 7.7 7.6 7.8 6 .0 Co nlrol, C onduc livity (11mhos/cm) 321 318 3 19 326 3 17 324 320 Non* lrc:ttcd t\ llmlini tv (mgn_ C:i C OJ) 61 62 63 ll nrdncss (1111!/L C :1C O,} 88 82 86 Tc1n1>er:uure ("CJ 24 .7 24 .8 14.7 2*1 s 24 7 24 .6 24 .6 24.'I 24 .6 24 3 24 7 24,5 24 .S 24 .4 11111<;; 11 1 8. 19 7.93 8.06 7.65 8.06 7.69 s.os 7.8 1 S. 10 7.55 8 .06 7.83 8. 14 7.57 no (m~n .1 7.9 7.6 7 .9 6.8 79 7.2 8.0 7.7 8.2 6.5 7.9 7.5 7 .9 6 .2 0 11l rol, ConductMtv (11mhos/cml 322 325 319 326 3 12 3 17 321 V- trence!I t\lknlinily (mf!/ L C:lCO,) 61 64 62 Hardness (nt t:/L C uCO, ) 88 80 80 T em uer:llure (°C) 24 7 24 .6 24 .6 24 .7 24 8 24.2 24 8 24.2 211.s 24.4 24 .7 24.4 24 .8 24 .2 nll ("Ill 8 18 7.89 8.09 7.6 1 8.07 7 58 8.06 7 74 8. 12 7.5*1 8.09 7.84 8. 16 7A9 DO(m ~L) 7.9 7.6 7.9 6 .7 79 7. 1 8.0 77 8. 1 66 8.0 7.5 7.9 6.:! 10.7% Co nductivil\' (1rn1 hos/c111) 309 3 18 306 3 10 295 304 305 Te m11r r:llure ('C) 248 24 .9 24.7 24 .7 2*19 24.4 '.!4.9 24 5 24.') 24.6 2tl.8 2*1.4 24 .9 24 .4 11111 !SU) 8. 16 7.87 8. 10 7.6 1 8.0 8 7.5 7 8.04 7.77 8. 11 7.56 S.08 7.83 8 . 17 7.48 DOim~/Ll 79 7.6 7.9 6. 7 7.9 6.6 8.0 7.7 8.2 6.7 8.0 7.6 7.9 6. 1 2 1.Jo/.. Co nd uc livi t\' (µn1hos/c111) 29 1 305 292 296 283 297 '.!93 Te111 11ern1ure ("C) 24.8 24 .7 24.7 24. 7 25.0 24.4 2 5.0 2~ . 5 25.0 24.6 211 9 24.3 250 24 .4 11>11 (S U) 8. 111 U7 8. 10 7.61 8.08 7. 57 7.98 7.77 8.0'l 7 54 8.09 7.84 8 18 7.49 DO(mi!/L) 7.9 7.5 7.9 6. 1 8.0 6.5 8.0 7.7 8. 1 68 8.0 7.6 7.9 6.0 42.8"/., Co nrluccivil v (ua1hos/cml 260 267 256 261 250 263 '.!56 Temoeralurc t"C) 2<1.8 24.9 24.8 24 .5 2 5.0 24.0 15 .0 24.6 25.U 24 .6 24 9 !4. 5 15.U 14 .2 11 11 (S UI 8. 13 7.85 8. 11 7 59 8 09 7.57 7 99 7.77 8. 10 7.58 8 . 0~ 7 86 8 19 7 .49 8~ . 6 *~. DO ( nll.!.IL) 8.0 7.5 79 6 z 8.0 6.7 8.0 7.7 SI 6.6 8 I 7.6 7.9 6 .0 Co nduccivic v (11a1hos/cm) 186 191 186 193 187 193 19 1 Tcnm<ru lurc (' Cl 1-1.9 24 .9 24 9 24 7 2 5.0 24 .6 2 5. I 24 .6 25.U 247 24 .9 24.2 2 5.U 24 .2 nil rS UI 8. 15 7.84 8.0 5 7. 5 1 8 04 7 56 8.03 7.76 8.0-1 7.56 8.06 1.87 8. 19 7 .48 DO ( m ~Ll 8.0 7 .5 7.9 6.2 8.0 6.8 8.0 77 8. 1 67 80 7.6 8.0 S.9 Co nduclivil1* (11 *1 hos/c111l 160 165 160 167 165 169 166 100% Alka linit y ( mg/I, CnCO,) 59 65 69 ll nr1lnes5 ( mglL CnCO J) M 66 66 *Total n csidun l C hlorine 11111!/L <0. 10 <O 10 <0 . 10 Tcmpcra lu rc ( Cl 25.0 2<18 24.9 24.8 25. 1 24.6 25. 1 24 5 25. 1 24 .5 2 5.U 24.2 25 . 1 24 .6 lu ll (SUl 8. 17 7.85 8.0 5 7.6 *1 8,03 7.53 8.05 7 78 8,07 7.62 8.07 791 8. 18 7A8 DO (m11/t.) 8. 1 7.6 8.0 6.9 8.0 6 .9 8.0 76 7.9 66 8 () 77 79 5.9 Condur1 ivitv lua1hos/rn1\ 163 165 158 168 16 1 170 167 100% lnt nk< Alkalinity (mgll. CnCO,) 61 65 67 ll :mlricss ( mgfL C11CO,) 64 66 70 *Tomi llcsiduol Chlo rine (m!!.IL <0. 10 <U. 10 <0. 10 Tcmueru curc ( Cl 24 .9 24.9 24 .9 '.N .5 25. 1 24 4 2 5.0 2-1.6 25. 1 24 7 25 0 24.5 25 1 24.5

  • Nole- ro1nl rcsnlunl chlonnc wos performed o n non-irenlcd Oulfnll IOI and l mak~ *ampks File: sqn101_05021 7chem.xlsx Entered by: J . Sumner Reviewed by: _ _ _

l Page I of7 Chronic Whole Efflu ent T oxicity Test (E PA-821-R-02-013 Method 1002.0) Species: Ceriodaplmia duhia Client: Tennessee Vallev Authori tv Co unty: Ha milton Facili ty: Seguovah Nuclea r Plant O utfall: 101 NPDES #: T N0026450 Project #: Difutio11 preparation i11form atio11: Comments: Dilution prep (%) 10.7 21.4 41.8 85.6 100 Efnucnl volume (ml.) 267.5 535 1070 2140 2500 Diluent volume (ml) 2232.5 1965 1430 360 0 I Total volume (ml) 2500 2500 2500 2 500 2500 Test or a11ism source in fo rmation: Test i11fo rmatio11: Orounism aoe: < 24-hours old Randomizin n tern late color: Date and times organisms were bom OS*d\.*\"\ Ola~'> TO 0'\()0 lncubntor number and shelf t--b_cl_we_c_n:_ _ _ _ ___,-,.--.,,.--.,.......-'----....-,...---=--..-- ---t locntion: Culture beard: oS-o\*t'\ Daily re11eival information: Day Date Test initiation a n d feeding, MHSW Snmple numbers used Analyst renewal a nd feeding, or batch used Outfa ll 101 In take termi nation time 0 05-02-17 O~"l.() o\o\*U--t1 ~ l '"\tfS.11\ . (J"t..,. \"\OSO \. o..\ At I 05-03-17 C>r1..~ o-\*'\le*O & \""\~O L O"b n.Oi"o\.O"\ A-<- 2 05 17 f'lF.."\.." o"\*11-* r\ ~ L\OS.~ *~S \"\O\~ . ~~ j{ 3 05 17 O d~ o~*t'->*l'1 e, \1. OS(]T) * ~~ l \ l>~a\,. ~I. }( 4 05-06-17 o& 3 <;. (J ~

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5 05-07-17 o~~o O~*II\.*\1 \1OS.OS.\1 \"'lOS~. \& i 6 05-08-1 7 l)&1.\ OS*<n.*\"\ \ '\Cl~I)~ * \~ l10CS.cS'. \i j1 - 7 05-09-17 ot'l..\ - ~ Co11trol iliformntiou: S ummary of test endpoints: Cootrol-1 Cootrol-2 Acee la nce criter ia  % of Male Adults : 01 . c 7~ S20% 7-day LCso  % Adu lts having 3' Broods: lCf)1. l0(:)1. ~ 60% su rv iving adults NO EC  % Mortality: ~1. 1. S20% LOEC Mean Offs rino Female: 3 .2 ~\.""' ~ 15.0 offspring/female C hV %CV: c.c..~7. s . ~1. < 42.0% lC2s SOP AT! I - Ex11ibit ATI 1.2, revision 11-01 -14 Page 37 of94 l \ - - - - - t-.dl.iJMJNJ~Llrl(. _,.) Page 2 of7 Species: Ceriodapl111ia duhin 1 Client: T VA I Sequo ya h Nuclear P la nt. Ou tfa ll J OI CONTROL-1 S urvival mu/ R eproduction Data Da te: 05 17 Rep licate number Day I 2 3 4 5 6 7 8 9 10 I Young producctl () 0 0 D 0 0 0 0 0 u Adult mor tnlity L. '- \._ '- '- '- '-- '- '-- \_ 2 Young prod ucetl ("") ("'} a ("\ t'I (') c 0 c\.,_ 0 Atlult morln lily \__. '- \__. \._ '- '- '- '- \..._ 3 Young pr odu cctl 0 ("\ f"'I 0 (') 0 0 0 0 0 \...- \..__ L. \_ \_ At.lull mortality \.... \..._ \.._. '-- \..._ " \'ou nr; prod uced ~ \.., l..\ s '5 s I..\ s "!:> '5 Ad ult morlnli ly L L '- '-- \.... \.,_ '-- '-- L \._ 5 Young prod uced l.C n \Q \. 'l.... \ 1.. I~ \Q I t> \~ \ \ Adult mortality \._ \.._ '- '- "-- \._ \.__ '- \.._ "- G Young produced n 0 D 0 0 0 0 0 () () 7 Adult mo1*tnl ily Young pr oducctl "- L '-- \I.. \_ '~ '- \ "?::> '-- ' '5 ,, '---- '- '\e '- \., L- -\ L. '(.,, Total young produced ~'"L.. ~~ ~~ "1~ ~t> ~1. ~~ ~b ~ -z_ ~~ Fin ni Adu lt i\lortnli lv '- '- '-- '- '-- '-- '- '-- '-- ~ X fo r J'd Urooib ')£.. ")':-. ~ ")C- )<. "Y- ')(:.. 7'--- I< >::- Norr:: Adul1 mort:rhly (l - h\*t', 0

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Co11cei1tmtio11:  % Mortality: I 01. Mean Offsp ring/Female: I 3l.'\.... CONC: 10.7% Survival and R ep roductio11 Data Rcplicnlc number Day I 2 3 5 G 7 8 9 10 D "' 0 I Young p roduced 0 0 0 0 0 (') 0 0 Adult morlAlity L '- '--- \._ * \..._ \_ L '-- '-- L 2 You ng protlu cctl I"\ ("', (\ o* n 0 0 t'i tJ 0 Adult mor rn lity '-- '-- \..__ L \..._ L L '- '- L J Young p rod uced (\ 0 0 0 0 () 0 0 0 u L L L- L L \.__ L L Atlult mnrlnli ty L '- .j Young produced s .... s L ~ ~ L- '..\ L 4 '{ s 5 Ad ult mortn lity L. L L L L L L 5 Young prod uced \ l.. \ 1... I\ ~ "'!> '-'\ \ '- - \\ n .. -"' \ Adult mortality \.._. '- '- '--* L \.__ L L- L G Young producctl 0 I) /) r., {j 0 0 r., 0 () Ad ult mortnll ty '-- \_.. '-- \.- L.. '-- ' - '-- '-- \..__, 7 Young protlu cetl \i l &' I'"\. \e \I I~ \ \o \ l$ I I..\. ~J Total young prod ucctl  :!> 1..\ ~'-\  :!> k. 3\ ~~ 3\ ~s ~\ ~\ '- L-. L.. '-- '--* L.. ~ '- '-- final Atlulr Mortality '-- .Vot~: Adult nte)tl31it (L .. lrt-c. D ::s dcl d), sn . sphl brood ( 1msfc brood spl.11 bc1\\CCR l \\'O dJys). CO - c.lrrv O\'Cf (o sonnc. a.me O\'Cr \\1l h .l du It dumi: lDM r.) er . Co11ce11tratio11: %Mortali ty: 07. Mean Offspring/Female: 3L.f  % Reduction from Control- I : -s. \?. SOP ATI I - Exhibit ATI 1.2, rev ision 11 14 Page 38of94 Page 3 of 7 Species: Ceriodap/111/(( tlubi(( Client: TV A I Sequoyah Nuclear Plant, Outfall 101 Date: 05-02-17 CONC: 21.4°/0 Survival and Reproduction Data Rcplicntc number Day I '.! J 4 5 G 7 8 9 10 I Young prod uced 0 {) n 0 0 n 0i 0 0 0 Adult mortnlity \._ '- L... \..._ '- '- '- '- '- 2 Young produced ("'} ,..,'- 0 n 0 {) CJ 0 0 n Adult mortality \..... L '- '- \_. '- '- \..... '- '- J Young produced ('} n 0 0 0 0 0 0 0 0 Adult mortality L \.... '- '-- L '-- '- '- \.._ L 4 Young produced '5 '- '-\ '-\ '"-\. s '--\ '"'\ "'\ l...o Adult mortality '- '- '- \.._ \..... '-- '- '--- '- 5 ' Young produced Adult mortality \~ '- \ !t '- '- \ ,_ \~ '- '- \C '- \::, \_. \ "1.. L '- \() '-- \\ '0°' \.._ 6 Young produced 0 0 D 0 0 0 0 0 ri 7 Adu lt mortality Young produced L \°' \., '- '- \-s '- \ \.o L.. \ C( L. \ \c, ,~ \.._ \ ~ '- '"\ '-- ,, '- Tomi young produced  !>-"'\ ~'° ~\ ~::, ~1. ~'-\ b\ ~~ "b ,_  :;.\ \._ Finni Adult Mortality \._ \..__ \..... '- '-- '- \..... \..... No1..*: 1\dul1 mort;,hty (L

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'- Co11ce11rr111iu11:  % Mortal ity: 01. Mean Offspring/Female: ~~.lo  % Reduction from Control- I: --i:1't CONC: 42.8% Survival mu! Reproduction Data Rcnlicntc number Day I 2 J 4 5 6 7 8 9 JO I Young produced 0 0 0 n () 0 (') 0 (') () Adult mortality L '- L L L L L L L L 2 Yo ung produced 0 \._ ("'\ f""I 0 t\ ~ 0 a 0 \,.._ Cl Adult morrnlity \....._ L \._ \....._ \_ \_ \..__ '- 3 Young produced 0 0 t"\ 0 0 0 0 n 0 () Adult mort:ilily '- L- L L L L L L L \.._ 4 Young produced ~ 5 L. s s "'\ ~ s .... l:... c_ Adult morrnlity '- '- '- \..... '- '-- '- '-- ~ 5 Young produced ' ""l.. ~~ \ "'l. \ ""l.. \ '-\ \ t> -\ \ '?:> \ "l.. \~ Adult mortality L \_ L L \.......- '-- . '- \..... '- \..._. 6 Young produced 0 0 i\ 0 0 ~ 0 a ('\ 0 Ad ult mortnlity \_ \..- L \.._ \....... L L L 1.... L. 7 Young prod uced l '\ \8 'l...C \S \$ 't:\ '\c. \~ \ '\ \-s Total young produced ~l.

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Finni Adull i\lortnlity L \...... '-- ..__ '- '-- .* '- '- '- '- Nau : 1\ duh moms1i1y (L

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Co11ce11trntio11:  % Mor1ali1y: 01. Mean Offspri ng/Female: 3~.'t  % Reduction from Control- I: - ft>.~7. SOP ATI I - Exhibit AT ! 1.2, revision 11 14 Page 39 of94 \ - -- - - _,, c...1n.NN1'Ul rutlnqklv:l.:l* La...:.. Page 4 of 7 Species: Ceriodap!tuia tlubitt C lien t: TVA I Scqu ovah Nuclear Pla nt, Ou tfall 101 Date: 05-02-17 CONC: 85.6% S urvival and R eproduction Data l{en lica tc number Da y I 2 3 4 5 6 7 8 9 10 I Young produ ced [) 0 CJ 0 0 (\ 0 l°'I a 0 Ad ult mortality L L '- L \_ '-- '--- '- '-- '- 2 You ng pr oduced 0 () A {) (\ 0 (') 0 0 n Ad ult mortali ty \ '-- L \_ '-- \._ '- \_ '- .__ 3 Young produ ced fl 0 0 0 0 0 a 0 0 0 Adult mortality L- '- '-- \_ '- L L '- \..... \.. Young produced '- 5 -::. '-\ l.. $ 'S ~ \..\. " Adult morta lity '-- '- '- \_ '° \_ \_ \....... '-- '- '-- 5 Young produced \~ \ '"L \C..\ \~ \\ \~ \~ \ '°';:) \~ l\ Adult morta lity '-- L \...... '-- L \__. '--- '- \....... \._ 6 Young prod uced 0 0 () 0 0 0 0 c 0 0 Adu lt mortality L '- '-- L L. L- L- \..... \,_, L.. 7 Yo ung produced \ &. \~ \~ \ '\ l\ \~ 1..0 \ .i. 1..o l\ Total young produ ced  !:>"""\ ~~ ~--\ ~~ ~1... ~~ ~i ~lo ~'\ ~1..:.. Fi na l Ad ult J\'iortalil)* '- '- '- .__ '-- '- '- '-- '--- '-- Not( : Aduh moni hty (L

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Co11ce11tration:  % Mortality: n1 Mcon OfTspring/Female: ~.'\  % Reduction from Control- I : -'\S. \"l. CONC: 100% S urvival a11d R eproductio11 D ata Rcolicn tc nu mber Day I 2 3 4 5 6 7 8 9 10 I Young prod uced a ('> (\ n 0 () (') ('., (\ 0 Adult mortali ty L '-- L L '-- L L '- \_ L.. 2 Young produ ced () 0 0 0 0 fl (\ 0 (""\ (1 Adult mortality *\...... '- '-- \..._ '-- '- \...._ '- L \..... 3 Young produced n 0 0 0 0 0 0 0 0 0 Adult mortality L. L- L L L L L L.. L L -I Young [Jroduccd '-\. s .s ~

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Ad ul t mo rtn lity '- - ' '-- "- L L \... \._... '-- 5 Yo ung prod uced \~ l'-\ ' ...l \ ,_ \'-\ \"b -\ \\ '""L J 1-Ad ult mortn li l)* .,, '- '- '- '- '- '- '- L.- '- 6 Yo ung produced 0 a () 0 () 0 () 0 aL ('"'\ 7 Adu lt mortality Young prod uced i..\ \__.. \""\ L \i ~ '2 L \~ l-- L 10 \.. L"\ L.. l '\ 1...0 .., \...... Total youag proiluced ~& ~le ~.., ~'- 31 3'\ 3'\ ~s ~~ ~~ Final Adult Mor tnlity .__ '-- \._.. \...__. \,_ ' - '-- '-- '--- \.._ NOii*: Adult monallly (L 11 lwc, D . d~d), SD .... split brood {tin!'JC brood spltr bc1wccn l WO d.iys:). co - Q n O\'Cf {offs'2rirut am cd ovt'.r with ~duh durinc. lransfcrl Co11ce111ratio11:  % Mortal ity : 61. Mean O ffspring/Fema le: 3'o.~  % Red uction from Control- I : - \\ .'\ T. SOP A Tl I - Exhibit AT I 1.2, rev ision 11 14 Page 40 of94 l Page 5 of? Species: Ceriodnpl111ia dubia Client; TVA I Sequoyah N uclea r Plant, Outfall 10 1 Oa te: 05-02-17 CONTROL-2 Survival and Reproduction Data Replicate number Day I 2 3 4 5 6 7 B 9 10 I Young produced (") 0 0 () () n 0 0 0 0 Adult mortnlity '- '- ' - '-- L L L. '- '- '-- 2 Young produced 0 0 0 0 0 () a () 0 n Adult morta lity ~ '- '-- '- "- \.._ '- '- '- '- 3 Young produced 0 0 D (J (', 0 (') 0 c n Adult mortality L \..._ L- '- '- '- L- L_ L L... .j \' oung prod uced w.. "-{ "-\ ~ "'-\ "-:\ '-\ s "-\ Adull mortnlily '- "- \._ "' '- \~ L ~ '-- '- '- \,_ 5 Young pr.oduccd Adult mortnlity l '- "- \ "'\... \._ \~ "'- \..._ \\ '-- - '-- '- \0 \0 \.... '-- \0 ._\\ G Young pro du ced 0 () 0 0 ('\ (j C) 0 () () Adult mort:tlity \,_ \.._ L \..... '-- \..._... '- L ~ L 7 Young pr oduced \""\ \S \S \~ \~ \~ 1'-1, \& \~ I \o Total young produced

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Co11ce11rrntio11:  % Mortality: 01. Mean OITspringlfemalc: ~~.(,,  % Reduction from Control-2 : - '\.$1. SOP AT I I - Exhibit ATI 1.2, revision 11-01-14 Page 41of94 T VA I Seq uoya h Nuclea r Pla nt, Outfall IO I May 02-09, 2017 ~ (Q Ve rifi ca tion of Cerint!apf111ia Rep roduction To ta ls CD EmironMt n t"I T0t.Ung 5olu1Jon'lo. Inc. ~ "> Conl.-ol-1 85.6% Ill' 1licu ll' uumlJcr ltcolle111c num bet .... 0 co lluy I I 0 0 2 J 0 J 0 s 0 6 0 7 0 8 0 0 9 10 0 Totnl 0 Duy I 0 I  ? 0 J 0 4 0 5 0 6 0 7 0 K 0 9 0 10 0 Toto! 0 ~ l 0 0 0 0 0 0 0 0 0 0 0  ? 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 u 0 0 0 J 0 0 0 0 0 0 0 0 0 0 0 J J 6 J s 5 5 J 5 l s 46 4 6 s s 6 4 6 s s 5 4 51 s 10 II 10 12 11 13 10 10 13 ti 112  ;; 13 12 14 IJ II IJ 13 13 14 II 127 6 0 0 0 0 0 0 0 0 0 0 0 G 0 0 u 0 0 o I o 0 0 0 0 7 16 16 14 13 15 17 16 I? 14 16 ISJ 7 IS iK 15 19 17 19 I 20 IS lO 17 18 1 Totol JO JJ 28 JO 32 35 JO J2 JO .)2 Jll 1'0101 J7 JS 34 .)8 n JS I 38 J6 J9 J? JS9 I0.7% 100% Ht111ic:11r uumlJ'-'r n cullnk 110mbt!r ll") Toll11 00) Tomi I 2 J J s 6 7 s 9 10 I 2 J 4 5 6 7 8 9 10 I 0 Q 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0  ? 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 u 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 J u 0 0 0 0 0 0 0 0 0 0 J 5 .1 s 5 3 ,j .j 4 5 5 *14 J 4 s 5 6 s 6 (> s 6 .1 n s 12 l<I 12 II 13 l'I 12 2 II 12 123 5 13 1.1 14 12 14 13 l*I 11 I! 11 129 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 18 14 18 17 13 16 <) I) IJ 161 7 21 17 18 IS 18 20 19 19 w 17 187 Tomi 3J J6 31 JJ J3 JJ J? J; JI JI J 28 1'01~ 1 38 J6 37 36 37 39 39 JS 38 .l3 3611 2 1.4% Contro l-2 lt t-nlicnl~ numbtr ll cn licntc num)er Uoy Tomi Dny To1ol 1 2 J J 5 6 7 8  ? 10 I 2 J J s 6 7 8 ~ 10 I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0  ? II 0 0 u 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 l 0 0 0 0 0 0 _g 0 0 0 0 3 0 0 0 0 0 0 u 0 0 0 0 J s (1 4 4 4 5 .j *I .j 6 4(1 4 4 4 4 6 s 4 4 4 s 4 J4 5 IJ 1:; 11 1:; IO 13 12 10 11 IJ 121 5 12 12 13 13 II 12 10 10 10 II 11*1 6 0 0 0 0 0 0 I) u 0 0 0 6 0 0 0 0 0 u 0 0 () 0 0 7 19 17 15 16 18 16 15 19 17 17 IC.9 7 17 15 IS 15 18 15 14 IS 15 IC. 158 Tolul 37 36 JI JJ 32 JJ JI J.) .ll 37 JJ6 Tot nl 33 31 )2 JJ J4 JI ?8 J2 JO JI 316 42.8% !Oil% Int ake n~ )licn ll' n111nbcr Ht111ictllc num brr Uny Toto I O*y Totnl I 2 J J 5 6 7 8 9 10 I 2 .) J 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 J 0 0 0 0 0 0 0 0 0 0 0 J 0 0 0 0 0 0 u 0 0 0 0 J J s 5 5 4 4 5 6 5 s 48 4 6 6 s 6 4 J 4 5 5 s so 5 12 13 12 12 14 10 14 13 12 13 125 13 14 14 124 6 0 0 0 0 0 0 0 0 0 0 0 ,5 0 II u u 12 u 12 l1 12 0 u 12 0 IJ 0 11 0 0 7 19 Ill 20 15 15 IQ 16 15 19 IS 171 1 19 17 17 19 19 15 19 15 lb 16 172 Tol*I JS J6 J7 J2 JJ JJ JS JJ JG JJ J44 Totnl J8 3~ J6 J7 JS JI 37 J? 34 32 346 .... ..f--r* TVA I Sequoyah Nuclear Plant, Outfall 101 May 02-09, 2017 Chronic Whole Efflu ent Toxicity Tes t (EPA-821 -R-02-013, Method 1002.0) Ceriodup/111i11 dubia Chronic Whole Efflu ent Toxicity Test EPA-821-R-02-013, Method I002.0 Qua li ty Control Verifica tion of Data Entry, Calcula tions, and St*1tistical Analy cs Environme ntal Te sting Solu tl on s, Inc. rrojcrt number: _ _ _ _ _ _ _ _ _ __ 12_J_J_2 Concc 11 lrn tion Reulicn tc num ber Sunri\'n l ,\ vcrngc reprod uction Cotffiri trH or f'rrcrnl rrtlnc1ion from v"ri:uio n ( 'Y* ) 1moh."4.I ron1rob (*!.) (%) (%} (orr, prini:lfcrnnle) I 2 3 ~ s 6 7 8 9 10 C()ut rol - I 30 33 18 30 J2 35 30 32 30 32 JOO 3 1.2 GA Not appliru ble 111.7% \4 3r, JI 3J JJ 31 '2 J5 I 31 100 32.11 5.7 *5. 1 21.4% 37 36 31 33 32 34 JI 33 32 37 JOO 33.6 6.9 -7.7 ~? .8 % 35 36 37 32 JJ 33 35 J4 36 33 100 3H 4.8 -10.J 85.6% 37 35 34 38 J_ 38 38 36 39 32 JOO 35.9 7.1 - I S. I 100% 3 36 37 36 37 39 39 35 38 33 100 36.8 S. I -17.9 '0111 rol - 2 33 31 32 34 34 31 28 32 30 31 100 Jl.6 5.8 Not " lllllic:i blc IUO% l111 akc 38 J4 J6 37 r JI '7 32 34 32 IOU JM 7.0 -9.S Outfoll 10 1: MSD = Minimum Signilicant Diflerencc llu1111 et1 '

  • i\ I 'D v:1luc; 2. 113 PM D "' Percent Minimum ignificant Difference l' MSD: 6.8 PM Dis 1.1 measure oftest precision The PMSD 15 thc nnmmu111 percent d11Tc rcncc between lhc control and lrcmment that con be declared stu11st1cnll s1gnilicant in a whok clllucnt toxic1ty test.

Int *kc: llu 1111c11's ~ I S i) ,.,,1 11~: 1.663 Lower l'MSD bound dctcnn111cd by USEP/\ ( 1011' pcrccntlk) =13% l'M **n : 5.3 Upper PM D bound dclcnnincd by SEI'/\ (9011' ~1ccntile} = 47% LO\ er und upper l'MSI) bounds were determined from the I0th nnd 90th pcrccnlllc. rcsp..'Ct1 vcly. or PMSD datu from EP/\'s WET !ntcrlaborutory Variabi lity tudy(USEP/\. 2001a. USEI'/\. 2001b) llSEPA 2 Ill. 2rHll I , Fi11:1I Report l111crl11bomtmy V:1rinb1hl r Study r EPA ShC1n-tcrn1 Chronic mid Acute Whok Efll ucnl Tox icity Tc~l Methods. Vtilumcs I and 2-1\ppcndi ~ El'A-821 01-004 nmJ EP/\ - ~2 1 -B 05 LI, Environmcmul Protect ion Agency, incmnati. M. TV A I Sequoyah Nuclear Plant, Outfall 101 May 02-09, 2017 Statistical Analyses tnvl~H1~tf~l1119 SoMlori~ Ceriod:.pnnia Survival and Reproduction Test-Roproduction S1a11 Date. 51212017 Test ID: COFRCR Sarnple ID: TVA I SQN OuLrau 101 End Dal e: 519/2017 Lab 10. ETS-Envir. Tesling Sol Sample lype* OMR-Oischarge Monitoring Repon Sample Date May 2017 Pro ocol. Fll\iCHR-EPA-821 -R-02-013 Test Species: CD-Cenoaaphnia dubia Comments: Non- Lrealed Cone *% 1 2 3 4 5 6 1 8 9 10 Con lrol-1 30.000 33.000 28.000 30.000 32 000 35.000 30.000 32.000 30.000 32.000 Co... irot-2 33.000 31 .000 32.000 34.000 34 .000 31 000 28 .000 32 000 30.000 3 1.000 10.7 34.000 36.000 31 .000 ~4 . 000 33.000 31 000 32.000 35.000 31 000 31 . 000 21 .4 37.000 36.000 31 .000 33.000 32.000 34 000 31 000 33.000 32.000 37.000

42. 6 35.000 36.000 37.000 32 .000 33.000 33 000 35 .000 34 .000 36.000 33.000 85.6 37 .000 35.000 34.000 38.000 32.000 38 .000 38.000 36.000 39.000 32.000 100 38.000 36.000 37 000 36 .000 37.000 39.000 39 .000 35.000 38.000 33.000 I ntake 38.000 34 .000 36.000 37 .000 35.000 31 .000 37 .000 32 .000 34.000 32.000 TrQnsform : Untf'ans formed 1 -Tailod Isotonic Cone **/o Moan N-Moan Moan M in M:>x cv~. N t*Stat Crl tlcal MSO Mo~n N-Moan Conlrol-1 31 .200 0 .9873 31 .200 28.000 35.000 6 .375 10 34. 117 1.0000 Control-2 31 .600 1 0000 31 .600 28 .000 34 .000 5 .816 10 10.7 32 800 1.0360 32 800 31 .000 36.000 5.713 10 -1 .732 2 .287 2. 113 34. 117 1.0000 2 1.4 33,600 1.0633 33.600 31 .000 37 000 6.902 10 -2 .598 2.287 2.113 34. 117 1.0000 42.6 34 .400 1.0866 34 .400 32 .000 37.000 4.766 10 -3.463 2.287 2. 1'3 34.117 1.0000 85.6 35.900 1.1361 35 .900 32.000 39 000 7 126 10 -5.087 2.287 2.113 34 . 117 1.0000 100 36.600 1 1646 36.800 33.000 39000 5.092 10 -G .061 2.267 2. 113 34 117 1 0000 I ntake 34 .600 1,0949 34.600 31 .000 38,000 6 .974 10 Auxilia!)'. Tes ts St:ttistlc Critic-al Skow Kurt Kolmogorov 0 Test lndicales normal distribution (p > 0 .01) 0.74361 1.035 -0.0134 *0 .8025 Bartlett's Test Indicates equal variances (P = 0 .81) 2 26348 15.0663 The con trot means are not s ignificenU~ dfflerent !e = 0.6 51 0.467 1 2.10092 H~pothos l s Test p -1<111 , 0 .05) NOEC LOEC ChV TU MS Ou MS Op MSB MSE F-Prob df Ounnett's Test 100 >100 2 11 279 0.06772 41 9367 4.2685.2 1.0 &06 s. 5~

TrA~t mnnf$:. v~ C; nn t rnl~ 1 Lino;ar lntorpol:>tion (200 Resamplos) Point  % so 95% Cl Skow IC05 >100 IC10 >100 IC15 >100 1.0 . . . - - - - - -- - - - -- - . , IC20 >100 09 IC25 >100 08 IC40 >100 0 ,7 IC50 >100 0 .6

i: 0.5

~ 0 .4 ~ 0 .3 ~ 0.2

0. 1 .

0 .0 -~ *., 0 - --01 -0 .2 --0 . 3 +--~--..-~-~........~----1 0 50 100 150 Dose o/o Dose*Rosponso Plot <0 1- tafl, 0.05 level of significance 10 5 0 N fD 0 g ,, -" ~ "'~ 2  ! "'"' 0 c; .£"' "' 0 0 0 0 Page44 of94 TVA I Sequoyah Nuclear Plant, Outfall 101 - Intake May 02-09, 2017 tatisticaJ Analyse Envlronme11talTe:st..lng Snlutlons. Inc.. Ceri odaphnla Surviva l and Reproduction Test-Reproduction Start Da te: 5/2/2017 Test ID: CdFRCR Sample ID: TVA I SO N Outfall 101 - Intake End Date: 5/912017 Lab ID: ETS- Env1r. Testing Sol Sample Type: DMR-Discharge Monitoring Report Sample Date: Ma~* 2017 Protocol: FWCHR- EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dub1a Comments: Non -treated Cone-% 1 2 3 4 5 6 7 8 9 10 Control-1 30.000 33.000 28.000 30.000 32.000 35.000 30.000 32.000 30.000 32 .000 Control-2 33.000 31 .000 32.000 34.000 34 000 31 .000 28.000 32.000 30.000 31 .000 10.7 34.000 36.000 31 .000 34 .000 33.000 31 .000 32.000 35.000 31 .000 31 .000 21.4 37 .000 36.000 31 .000 ~:wuu :1£.UUU 34 .000 31 .000 33.000 32.000 37 .000 42.8 35.000 36.000 37.000 32.000 33.000 33.000 35.000 34 .000 36.000 33.000 85.6 37.000 35.000 34 .000 38.000 32 .000 38.000 38.000 36.000 39.000 32.000 100 38.000 36 .000 37.000 36.000 37 000 39.000 39.000 35.000 38.000 33.000 Intake 38 .000 34 .0 00 36.000 37.000 35.000 31 .000 37.000 32.000 34 .000 32 .0 00 Transfo rm : Un t ransformed 1-Taifed Cone-% Me an N-Mean Mean Min Max CV% N t -Stat Critical MSD Control-1 31.200 0.9873 31 .200 28.000 35 .000 6.375 10 Control-2 31 600 1.0000 31 .600 28.000 34 .000 5.816 10 10.7 32 .800 1.0380 32.800 31 000 36.000 5 713 10 21.4 33.600 1.0633 33.600 31 .000 37 .000 6 902 10 42.8 34.400 1.0886 34.400 32.000 37 .000 4 .786 10 85.6 35.900 1.1361 35.900 32.000 39 .000 7.126 10 100 36.800 1.1646 36.800 33.000 39 ODO 5.092 10 Intake 34 .600 1.0949 34 600 31 .000 38 .000 6.974 10 -3. 128 1.734 1 .663 Auxiliary T es ts Sta t istic Cri tical Skew Kurt Shapi ro-Wilk's Test indicates normal distribution (p > 0 .01) 0.94304 0 .868 -0.2144 -0.8437 F-Test indicates e qual variances (p =0.43) 1.72368 6 .54109 The control means are not significa ntly different !p =0 .65) 0.4671 2 10092 Hypothesis Test (1 -taif , 0.05) MSDu MSDp MSB MSE F-P rob df Hornoscedastic l Test indicates no significant differences 1.66325 0 .05263 45 4 .6 0.00582 1, 18 Treatments vs Control-2 Dos e-Respons e Plot 40 35 L-~4---f-~-t-~-r-- 1-tail, 0 .05 level .2 30 of signfficance -g 25 'C ec. 20 Cl> 0:: 15 10 5 0 N .... 0 ., g ~ 0 ~ ": ~ N ~ "'.... "'co .,.; 0 ..: .!!! c: ~ E 0 0 u (.) Page 45of94 Page 6 of 7 Species: Ceriodap/111ia dubia Date: 05 17 C lient: TVA I Seguova h Nuclear Plan t. O utfall 101 Daily C/J e111istr)1: Day (Analvst idcmificd for each dav, ocrformcd oH, 0 .0. and 1.:u11du1.:ti vitv 111ea5u1e1ne111s only.) 0 I 2 1'-1 J- - - A nalyst J-.- ., tlV ct-' -. - . -- e..oe-Concentra ti on Pn ra met cr . ~ . ., < * , ~ * ~ ~ -~ ..... pH (S.U.} A.21 '3 02. A .lb 01"h ~Ai-DO (m!!IL} 4-q '+..0 1-.f- --+~ ~-'1 '+.e Co nduct ivity .*. j(q ' (itm hos/cm) $Z.\

-- -*- 32.8 ._

*- *-.I ~M ' ,, . .- .. C O 'TROL

  • Allmlinily lo\ \o1.. ~4'\

(mg CaC01/L) *--; * ..I , - * .I:':. " . . .. - .. ~ *Hardncs .~ ::- , g~ .) a1-(m~ CnCO;IL) - . "'Tempera t ure (°C} '"l.'-\. lo "l~.o \.~-"\ '"\.."\ ."\ L...\..J "\S. \ pH (S.U.) e,l~ 0.0\ All P'll'\(') 'f>.~ '1-~~ 10.7% DO (mg/L) Condnclivity (im1hos/cm) __;01 ".)..~ ~~ ~.e

31~

t>rq " ':\-.0 2o'-( ... ')..~ . .. - . *Te mperatu re (°C} "b\*\ "¥... \ ~\:"\ -i,... \ . \ -i."\. ~ \c;. * .,_ oH CS.U.l .a,q e>.01 A.\2. /l.QG1 ~.10 '1R1-DO (m!?/L} 'l.a ~Q "")..£.\ 'I~$-/ ':\-.~ '*l.0 21.4% Conductivity - (µm hos/cm) Aq\ ~~p 2qo 1;; -_ c ~ Tcmncrnturc (°C) '-'\*\ L.~*~ \>\."'\ "'\..'\. '\ &.'-\. '\ '"\S."1... DH (S.U.) e.1e e,.")(') '-".l'2. ;iJ"ID 8 .t¥'J "'l.q~ DO (me.IL) '.}.q ~c::\ ~.~ ~9 ':\-.~ -':\-.e 42.8% Condncti "i!Y ~" ~ *, (111nhos/cm) ~Si- itl4'5 *.\ - 29./) - -. ~ "?.'-\* ~ "'\.'\-~ "1..~-~ ""~' "T empera ture (°C) "'\.'\-1 1.$. 0 1JH (S.U.) 9.\1. Pion ~Jn e.02. >-00 ',\_qg DO (m!?/L} '~q 1=l. q *+.C\ 't.q ....- .. ~e ~~ 85.6% Conduc tivity - .... -  ; ***- CC*: " (itnihos/cm) l>lP I* lOO - \61- . -,: .. .' " Temperatu re (°C) "1.."\* \ L"\-cf ,,4. '\ I..'-\ . f "'\.'V\ '1.~*1 DH (S.U.) e.1z.. ~~C\ e.111 A,l) l E'J.DS Ila~ DO (mg/L) a.o ~-" e.o qft { 4t\ PJIJ - ,,,,'.'. Conducti vity (11mhos/c111) t66 . .... \ltl'3 lee? r- * *Alkalini ty -- *: . ~- ~ .. - - ,:._ ' ~*--- 100% (mgCaCO.i!L) S'\ ' - \o~ *~ . ~ *Hn rtln c s -- ~ 'b-i.. \o\o (mg CnCO,IL) - . -- - . -<. O* lO -:,... --~ *TR chlo rine (mg/L) <o. lO ~ \ "!'-. "-~~- . *T empera t ure (°C) "'t~* l "l.S.\ L.'-\ * ., "\..'{. '\ -,s.o 1.'t. '1 pH (S.U.) E3.n2- "}P.~ e>.oo A..O\ e.oco 'tq~ DO (m ~L) e.1 r+.o, {:!)J) ~.e ~.<=\ '\Pe Conductivity . -.. , ,< ' ~.;* \~3 Ht>6' __. *' lll2.. I! .. (11111hos/cm) - .~ - ~ -' 100% *Al knlin i!y s~ -  !' "* '* - (.\  :- ~ --~'~' . Inta ke (m g CaCOJLl *llnrd ness ' . -. -*.._ J ' .- - - . -,.!- (mg CaC03'Ll lP'-\ J -* - -:..... .. \o\:i ,.,.  : *T R chlorine (mg/L) -'.o. lO ~ ,0. 10 . - - *-* " T empernture (°C) '"l.4-i l."l. C\ i."{. e 1.'\ .'t 1S.O ~*0 l ni tfal Fin a l l nit inl Fin a l Initia l Fin al *Tempera1ures pcrfonned at the time oftest initiation, renewal or tem1inntion by the analyst identified in the Daily Renewal

  • lnfomiation table locmcd on Page I. Alkalinity, hardness mid tot~I residual c 1lorinc perfom1ed by the analyst identi fi ed on the bench sheet specific for each anal ysis and transcribed to this bench 5hcet by: -...,M-T--

SOP ATI l - Exh ibit AT! 1.2, revision l 1-01-14 Page 46of94 ~ r;=~/Q l .. \ -. - ----- \ . ' ~ ll',....,.,rwl\,fl'UlfHdnqSohlllriftt.IM. Page 7 of7 Species: Cer iotlap/111ia dubia erten t: TV A I seauo va I1 N uc Iear Pl ant. 0 ut f a 11 10 1 0 ate: OS -02 17 - Ony (Analyst idcntilicd for each day__ per[om 1ed pH D.O. nnd conductivitv measurements onl y.) 3 4 5 6 V'- ~ 'I-- 1)-.J A.../ Ann lys t ,P,~1...- i>-- ~ Jv .1 l Conccn-trati on Pnrn metcr -. - - -  ; . .' ,- .. - ' ~ .:f . .'

.* * .. ., --'* '"" - ' .. 7 v- .,' pH (S .U .) tJ-i \~ ~ o4 ~ . n'l ~.G\P> 8.\~ P,,\.)(\ E:?.\Or. ~-01 DO (mg/L) +.~ ~-°' '"l .~ e..o.. - ~~ a.o ~.6 '}A.. Conductivity .. . ~ 02.J...O 32'--' - 320 .. ( ~1mhos/cm) I~ - -* 311-" -- - _j.. . . ,.. - --- *I" ., CONTROL *Alknlinity ~~ ~ . - ~~ - . -  ! "x.. ~~"' *-.~* - "-~...~**" l (mg CaC03'L) *Hardness (111~ CaCOi/L) u- ~ ~ - &l.. ~--- ~ ' . ' . ~ .. _,._.,,. - *Temp e rnturc (°C) \.'0.()1.o "" .~ ""t...!\*'\ ""\Y\. ci ,_"\ ."' "1.-\.* \ '\~. e.oe \ "\."\.' ""'\,C,.1.. .., oH (S.U.) i .o'+ 'B*\'2- Z\.c:\1- 0 .IL"'l B .\":}. 9, ,"q /?)~ ~.~ DO (111~/L) ~- e> ~ll -~ ~.o r:\A ~.'! 10.7% . *- Conductivity (µmhos/cm) Bco - - ~ :i-~qt? ~ ~°J J . .. _,\lo ~ 00~ -' , - - -- " Tem perature (°C) \.'-\.*'\ \.'S, \ "'\."\.'\ is."\.. '1."\. & '1.~.'\ 1.'"\*C\ '"\C. .o oH(S.U.) ~ *...a 'iS .04- '6. \ '2. ~Al" @,o g~ S.1A A.o-1 DO (mg/L) "':3-Pl 8'.o ~:<> 0.0 ... '+(:") 0 .0 ~-'\ ~.11 I  : ... 21.4% Cond uctivity (umhos/cm) 2,qz. .. ' .2. ~\p aoo ~ 2'10 *Tem pe:rntu r c (°C) 'i>\*"'\ -,,S. '\.. -'\.."\.'\ "1..C.. 'c "\.~*~ 1..\..:\ 1.."l*"\ '\.S..'\ pH (S.U.) ~ ~ .o<t- ~ . \\ J'\,q',}- Q.10 0 .<:A 0 .10\ ~ ,i< DO (m!!IL) ...g 3.0 li'.n 0 .0 r:\_O\ 0-0 ':} C1 ..~ -0 42.8% Conducti\'ily _,) ., (umhos/cm) 2<.Q l ~~ ~ ~I03 -- - ~51' ~ y ,;;: : '# " Temperat u re (°C) l.'\-'1 '1>\ . ~ "'\>\."\ L') .t:i '1.'\*i ""tS . O \..'\, .'\ I..\ .'i oH (S. U.) PJ.U~ i.o ~ '6.\2. . 4~":\- 0 .10 0.10 8.\'\ 0-<XP DO (mgfL) ':tA ~.\ ~.::<> PJ..O O.n ~.o i?J.u '3.o 85.6% Cond ucti,*ity ~'\ \ = -, \\'.(7._... ,_ '1 \C\W ..  : \6".f '" ' (11mhos/cm) "'Tcm p erntu rc (°C) ""\.~~ 1.J.\.k .. "\.'). 0 - ~. \ ~\.f . " \S.Q \.~.o .J "1.S.\ _. ~ pH (S.U.) .-:}~qs ~ .0$' ~.O'i-- 1 .t:\'2.. AOC\ ~-cc-1 ~.\'\ 8 .0 1 DO (m!!IL) , .+R. 'l\.")... ')!.. .... e. .\ - 0 .0 e.o e.o 8 .0 Conductivity ., ~~ . . *~ (µ mhos/an) ~l.06' \5'~ .... \1-\ ". *: . ' 11oo . - . . --- ~ ,. , . - '""' . -~ "Alkalin ity ~\ .. . ' 100% . " (mg CaCOi/L) - .---- - - --.,- * :! - >. *.c.. -- -"'

..,1. *

" Hardness .. ~-~ ~ ' ' *. - I .A' ~ - *<' I- ' ..,. '!' ' .. -.. . ~~"" '.;,c ...:.1..;_ (m!? CaCO;/L) - l* . ... ) -- -  ;  : - .. *~ ~' -* l *-ii::*: .. ... ~ -- - . ' ~TR chlorine (mg/L) -. "\)\. t O*lQ ~ . t> -~ "\.'"\.. ~ "  :.; ' 1..$. o ~ "\.'\. \' *Temper nturc (°C) ~.o "'\.'\.' '1S*1 pH (S.U.) B.oel "i! ';)$'" ~.\\ 0.0\0 ~ ., o ~. \\ e.1e e .11 DO (m!!IL) Conductivity () ~ . <j\.-z... ,. 'J. a r 0tJ - 0 .0 o,_n . . *p . , ei .1 6'.1 . .* ., - - ., }lP 3 l lo) l'}:t /""IO (11 mhos/cm ) 8 , ~- ..... _..;,_!""_ _..- *-. ' " !00% *Alkalinity ' ' t.,S ' ' lntnkc (mit CaCO;IL) *Hardness '"*

.....,

- - j (o~ --~ . ~1J,.~,-1J._ . , r -- . . ,- , (mg CaCOJIL) II f - j *- - .. .. -. .,  ; *TR ch lorine (mg/L) , > - * :>1 .::a.JO le ' *Tcmpernturc {°C) 1$ . 0 '"1.Y,J\ 1.':\.!'\ ~.o '1.Y.*~ '1.'i .o o.o 1."\ -~ I nit in I F in a l In itial Finn i Initia l F ina l l nitin l Final *Temperatures performed al the time of test initiation, re newal or tcrminauon by the analyst 1denufied Ill lhe Daily Renewal lnfonnation table located on Page I. Alkalinity, lmrdncss and total rcsiduald\orine p formed by the analyst iden tified on the bench sheet speci fic for each analysis and transcribed to this bench sheet by: . SOP AT I 1 - Exhibit AT! 1.2, revision 11-01 -14 Page 47 of94 TVA I Sequoyah Nuclear Plant, Outfall 101, Non-treated May 02-09, 2017 Ceriodap/111ia dubia C h ron ic Whole Effluent Toxicity Test EPA-82 1- R-02-013, Meth od 1002.0 Daily C hemical Analyses Environmental Testing Solutlons, Inc. l'rojcct number. _ _ __ _ _12_3_3_2 Conccntnition Parnmctcr Da v o Day I lhv2 D:w3 Dav 4 O:n-5 Du y 6 Initial Finni Initial Fi1rnl Initial Final Initial Fimtl ln ith1I Fi1rn l lnitinl Fi11:1I ln itinl Fin'11 lnll !SU) 8 21 8 02 8. 10 8 00 8 08 797 8. 13 8.04 8.08 7.98 8. 15 8.09 8 19 8.09 DO (me/Ll 7.9 78 77 79 77 7.8 7.7 7.9 7.8 8.0 7.7 8.0 78 7.9 Conductivil\* (11rnhos/cm) 32 1 328 3 19 316 317 324 320 ontro l Altmlinity (1111:/L CaCO,) 61 62 63 llartl11 css (mi:/L C11COJ) 88 82 86 Tc11111ernturc c"Cl 24.6 25.0 24 7 24.9 24.8 25. 1 24.8 24.9 24.8 24 .9 24 .7 25. 1 24.8 25 2 1>11 (SU) 8. 18 8 01 8. 11 8.00 8.0R 7 97 8.06 8.04 8. 12 7 97 S. 10 S.08 8. 17 S.09 00(1111!/Ll 7.8 7.9 i .8 7.9 7.8 7.7 7.S 79 7.9 8.0 78 8.0 7.9 7.9 10.7%. Cond uctil'ill' (umhosic m) 309 314 304 310 298 3 16 303 Tcm11crnturc ("C) 24.7 25. 1 24 7 24.7 24 9 25.2 24 .9 25. 1 24 .9 25 .2 24.8 2.\.9 24.9 25.0 oil fSll\ 8. 19 S.01 8. 12 7.99 8. 10 7.97 8.09 8 04 8 12 7.97 8. 10 8 08 8. 18 8.04 21.4 'Y., DO lmp/L\ 7.8 79 78 79 7.S 7.8 7.8 8.0 8.0 8.0 7.S 8.0 7.9 so Co111lnrlivit1* (umhoslcm) 291 293 290 292 286 300 290 Tcm ucratu re <C) 24 .7 24.8 247 24 .9 24.9 25.2 24 .9 25 .2 24.9 25 .0 24 .8 24.9 24 9 24 9 11111 (S ll l S. 18 s 00 8.1'.l 8 00 8 08 7.98 S.OS 8 04 &. 11 7.97 8. 10 8.09 8. 19 8.05 00 (1111:/L} 7.9 7.9 7.8 79 7.8 7.S 7.9 S.O 8.0 80 7.9 8.0 7.9 8.0 42.8'Yu 257 Co111luc1ivi11* fo111hus1cm) 265 256 26 1 248 263 257 Tc111 11cn11urc (°(.') 24 .7 24 .8 24 s 24 .8 24.9 25.0 24 9 24 .8 24 . ~ 25.0 24 8 25.0 24 .9 24.9 11111 CSlll 8. 17 8.00 S. 10 S.02 8.08 7 98 8.08 8.03 8 12 7 97 8. 10 g 10 8 19 8.06 DO (me/Ll 7.9 7.9 7.9 7 CJ 7.8 7.Q 7.9 8. 1 8. ~ 8.0 8.0 8.0 8.0 8.0 8!1.6"/., ICon1l11cti"i tv lumhoslc m) 186 188 187 19 1 182 196 187 Tc1111>cn1111rc ("C) 24 7 2.t.8 24 .9 24.8 24 .9 24 .9 24 9 24.8 2D 25 I 24 .8 25 0 25 .0 *25 I nil ISUl 8 12 7.99 8.09 8.01 8.05 7.98 7 95 8.05 8.07 7 92 8.09 S.09 8. 19 8 01 OO (mi:/I.) 8.0 7.9 8.0 7.9 7.9 8.0 7.9 R2 8) 8I 8.0 s.o 8.0 8.0 Co11ducti"it1* (µm hos1cm) 158 163 167 165 158 171 163 100% Alkn linity (mi:/L CaCOJ) 59 63 67 ll n rdncs~ (mr:IL CaCOJ) 62 66 65 Tomi llcsidunl Chlorine ( m!!IL) <0. 10 <O 10 <O I ~ Tcmocrntnrc ("C) 24. 7 25. 1 24.9 24 .9 25.0 24 .9 25.0 24 8 2'.i.) 24 .8 24 .9 25 .2 15 0 24.8 11111 (SUI 8.02 7.99 8.08 8.01 8.06 7 98 8.05 8.05 8 11 8.06 S. 10 S. 11 8. 18 8. 11 OO (m!!/I, ) 8. 1 7.9 8.0 7.8 7.9 7.8 7.9 8.2 7.9 8.0 8.0 8.0 8. 1 8. 1 Couductivitv l11mhos1cm) 163 165 162 163 165 177 166 100% lnt:ikc Alk.1 li11 ity (mJ1L CnCOJ} 59 67 65 ll nrtln rss (mJ:{I. CaCO,) 1\4 66 63 Tornl llcsilluul Chlorine (me!L) <O 10 <O 10 <0. 10 Tcmnrmture (°Cl 24.7 24.9 24.8 24 .9 25.0 25.0 25.0 24 ,9 .2-t9 25 0 24 .8 '.!5.0 .25.0 24.K File: sqn101_050217chem.xlsx Entered by: JJmner Reviewed by: Page _ __ Page_ /_ of _ (__ Envtronmentalli !.ting Solutlon 1, Inc.. Total Residual Chlorin e (ORION-1977) MalTix: Water, RL = 0. 10 mg.IL Meter: Accumet Model AB250 pH/mV/Ion Meter Analyst ~I/-1. -'S ----~, lodide reagent I JNR 1-97 Date analyzed os-

  • oz.. *I-:/. Acid reagent: /Nt 7?8 Calibratio11:

O.lOmg/L 1.00 mg/L mV C hn ngc (suggested rnngc = 26 to 30 mY) Reference stnndnrd num ber j)JJJ' J~~g /N.!.J' 1~-:rll Millivolts (mV) Sf,1 . 9 5*(,, 2'1.-:/ Note: For samples with a residual chlorine of > 1.0 mg/L. the samples musl be di lulcd to be with in the calibration range. L ll b orator11coutroI stn11 dart.: f Reference sta ndard True value (TV) Mensured va lue (MV)  % R = MV / TV x JOO number (mg/L) (mg!L) (acceptab le range : 90 to 110%) h.J~J J"171, 0.50 (l) . 5'16 loet. 2 7a r D up 1cate samp,I e prectswu: Sn mpl e Sample ID Sample chnrncteristics Residual chlorine  % RPO = {(S - D) /l(S+D)/21} x 100 number (me/L) (acceptable range =+/- 10%) \~1. b~ {l..'lf:I. Rik Jn.,, r.~ s I. 0 -000 Duplicate D /'T.f' j l. 0 -00L-f O&.H.z,.:T Sam l e 111ens11rem e11ts: Sample Sample ID Snmple characteristics Residu al chlorin e number (m /L) Reaacnt Blank l. O-oo"l ~ .o l c). 003 l1t:J::;D I. Q3 O - ooJ o . ooJ L. 0 . 002 l 0 - 0t.)} t () .c:J()J 1 o.ouJ Note: All samp les were analyled in excess of EPA recommended holding time ( 15 minutes) un icss otherwise noted . L a bora ory coutro sta11 darr.: I Reference sta ndard T rue value (TV) Measured value (MV)  % R = MV I T\i x 100 number (mg/l) (rng/L) (acccpta blc range= 90 to II 0%) Jl..q~ 0.50 />.J.sJ 0 .c;H JO? *" /(.? Revi ewed by Dmc revit:wt:LI I3*02..11- ~ Page 49 of94 OP C8 - Exhibit CS. I, revision 01-0 l- 13 Page C I Page_2_of~ l Envlronment-,JTesting Solutions, Inc. I Total Residua l Chlorine (OJUON-1977) Matrix: Water RL "' 0. 10 mg/L I Analyst Date analyzed 1.-/-J -f -----., OS'* o1 ., ~ Meter: Accumet Model AB250 pH/mV/lon Meter Iodide reagent: Acid reagent: I Calibration: 0.10 mg/L 1.00 mg/L mV Change (su csccd rn 11gc = 26 10 JO mV I . the samples must be diluted 10 be within the calibration range. I Laboratory control ta11dartl: Reference standard True value (TV) /-(/JD l I -:J-  % RS = MV I TV x 100 number (mg/L) I 0.50 I ample chnracteristics Residunl chl orine (m /Ll %RPO= f(S

  • 0) /l(S+D)/21} x 100 (11cccpt11ble rnngc ="' 10% )

s Lo. l)J"f I ,.....;i Duplicate D L o-ol Z. i---------- o.rvzr,. Samp.Ie 111easure111e11ts: I n m pie number Snmp le ID Sample characte r istics Residual c h lorine (mg/L) Reagent Blank . ,..~ ,..)"'tJ ?.I? ' I \1oSDZ. . \) \'JA\Pc!. l~I\\({ -~tLe. P:dt. .k ( -/ p.§&'1 (') . oO~ I / / / .A A (/ f1 / I - '-...J'._ -- ,,_i / 0 I / I I /-- Note: All sampl s were analyzed in excess or EPA recommended hold mg lime ( 1S mmutes) unless 01hcrw1se 1101ed. laboratory control standard: I Reference standard number True va lue (TV) (mg/L) Meas ured v:iluc (MV) (mg/LI  % RS = M V I TV x 100 (acccplllble rnn gc

  • 90 to 110%)

/JJ .J.J' h 11J 0.50 I Reviewed by Date reviewed I JJ oz A1-()5. I Page 50of94 SOP CS - Exhibit C8. I. revision 01-0 1-13 ' Page _.....__ Page _ I _ of _ '2_ EnYlro nmenl af Testing Solutions, Inc. Total Resill ual Cll lodue (ORIO -1977) Matrix: Waler, RL = 0. I 0 mg/L Analyst ,....1-1. Date analyzed -* -'-J ----- ., O ':>*D"T* /"l Meter: Accumet Model AB250 pHi mV/lon Meter lodi~e reagent : ~_,_J_Al_fi--'-1-_"7_1 ___ Acid reagent: L--._l.:..:>.J_,f'l---7~ - '19"'-------' -l Calibratio11: 0.L O mg/ L L OO mg/ L mV Change (suge.cstetl r:lllJ?C= 26 10 30 m VJ Refer en ce st andard n umber I }.,J.tJ 1'1 ?-8 /,41./.J' l "t'f'I M illivolts (mV) S":/2 *7' foe>). 2 28. fJ Note: l'or samples with a residual chlorine of > 1.0 mg/L, the samples musl be diluted to be w1l111n the cahbra11011 range. laborator,,- con trol stan dard: Reference sta n dard True valu e (TV) M easured va lu e (M V) =  % RS MV I T V x 100 number (mg/L) (mg/L) (acce pta ble ra nge= 90 to 110%) /ijfJ l'-t ;rg 0.50 Q . Q)3 }00 - 6 ~ .. D up/icate samp I e precision: Sampl e S ample ID Sample c ha ra cteristi cs R esid u:1l ch lor ine % RPO = {(S - D) / (( + D)/21} x. JOO number ( mg/ LJ (ncccplnblc ran ge - +/- 10%) A~f.. r~ s no~o'-\ .o\ ,, ~ *- rP~ - ..1 Lo . ooC. - ,...., 1 Duplica te D L,,. oot 0 .rt:1'?1'~ Sam le 111eas11re111e11ts: Sample S11 m p lc ID S nmplc chnrnctcristi c R esi du11I chlorine number (010/L) Rea ent Blank - o - 0 0'$" O* oo z L o - 003 0 - oo -Z 0 - 0 17 0 - 00 \ i o~ o'-\ . IT!:> l V - Ot> } L o -vo* Note: All samples were analyzed in e:.:cess of EPA recommended hold ing time ( 15 minutes) unless otherwise noted. E;\o\et~ll P1'i~o~~*\'"\ Lba oratory coutr o I stan dard : R efe rence

  • tand a r d T rue va lu e (T V) Meas ured va lu e ( M )  % RS = M I T V x 100 numb er (mg/L) (mg/L) (acceptable rn ngc = 90 to 110% )

I )J r,1 '"'17r8 0.50 /'n,~ ]"1 . k//'v Reviewed by ~--a i{..__ __---l Date reviewed '----'O:o.. . oc... So*..;:; lM_,_*J,_1....__ _. Page 51of94 SOP CS - Exhib it CS. I, revision 01 -0 1-1 3 Page _.....___ Page~_ of £nvlronmentall6tlng Solutkms# hu:. To tal R esidual C hlorin e (ORION-1977) Matrix: Water, RL = 0. I 0 rng/L . - - - - - -- ---.Meter: Accurnet Model AB250 pH/mYnon Meter Analyst IHJ Date analyzed 05 . O'-'/* / 3 I

Iodide reagent: Acid reagent: : I ...._ ~" rt/' 0 "<<..:. I Calibration: 0.10 mg/L 1.00 mg/L m Cha nge ( u2gcstcd ranJ!c = 26 to 30 m V) Laboratory co11trol stmulnnl: Reference standnrd True v11lue (TV) Mca ured va lue (M n umber (mg/L) (rng/L) 0.50 D up ./"1cate snmpI e prec1s1011: Sample Sample ID Sample characteristics Resid ual chlorine  %.RPO = {(S - D) / l(S..,. D}nl} x 100 number (mQ/L) (acceptab le ra nge =

  • 10%)

\""\oSo'-\.CS Dll~<<.(\~ PCl-4. h. rP1J11. t;,,J,?IP.; s 0 .1z.B L Duplicate D o . 1'l.(,. /.' ,0 samp e m easurements: amp le ample ID Sample chnractcristics Rcsidu:il chlorin e number (mii/L) ' Rea1?ent Blank I"-'(~ ~,..> t,0ScN . 6f t\.nru>1\eA~ u lfl l l,n, )/ CJ ,;,AA C>. O<>{, 7 I I I t-1 I I I riJ'-Ql-// 1-- v I I *~ No re: A ll samples were analyzed in excess of EPA recommended holding t une (15 mmutes) unless ot herwise noted. laboratory control standard: Reference stan dard True value (T ) Mens urcd v:i lue (MV}  % RS = MV /T x LOO number (rng/L) (mg/L) (acceptable range = 90 to 110%) /;)JJ I '-1??i 0.50 o . 48 1 9'- 2.jlo. Reviewed by Date reviewed I::::o:'#J'.-rs..I. . . -~o:'i:-:1-1~-----. . .,~ Page 52of94 SOP CS - Exhibit C8.l , revision 01-01 -13 Page t l --- I Page of I Env ronmcm t;. I Testing Solutlon t.. fnc. I Total Residual Chlorine (ORIO -1977) Matrix: Water, RL = 0. 10 mg/L Meter: Acc umet Model AB250 pH/mV/ lon Meter I Analyst [....-J.....J-- Date analyzed hS.t>>. *"l- [- ~-v-___,, lodi~e reag.ent: [IN l-3o/l Acid reagent: ~ ('l rl -=1"8 "-'-- - - " -=--- - - - ' I Calibratio11: O.JO mg/L t.00 mg/ L (S UJ?l?CSICd mV Cha nge ran c : 26 fO30 m V) Reference st a ndard number 'NSS1 Milli volts (mV) 5 Note: For samples with a residual chlormr. o f > 1.0 m L, th e 5amples must be diluted to ut: wilh in the c libration range. laborator

  • coutrol standard:

R eference sta nd a rd T ru e valu e (f V) Measured val ue (M V)  % RS = MV I TV x I 00 number {mg.IL) (mg/L ) (accepta ble rnngc .. 90 to 110%) 0.50 o. Du licate sam 1/e 'J recisio11: Sampl e Sa mple ID Sampl e ch a ra cteris ti cs Residual chlorine  % RPO = {(S - D) / f( + D)/21} x 100 numb er (m IL) (nccc ptnblc rnngc =;!; 10%) noso c...o Me...y._ ~ Duplicate Snm 'J/e meas11re111e111s: Sample Sample ID ample character is ti cs Residunl chlorin e number ( m ~/ L) Reaoent Blank O .c\\p < O.OQ .... b.co-:r <.:> *.QO\ Note: All samples \\ Cre analyzed in excess of EPA recommended holding time (IS minul cs) unless otherwise noted. laborator co11trol sta11tlnrll: Refer e nce stnndnrd True valu e (T V) Meas ured vn lu e (M V)  % R = M V I TV x I00 number {mg/L) (mg/ L) (a cce pt a bl e ra nge

  • 90 lo 110% )

0.50 q I~==o:.s::-:o~lo== J Reviewed by j{ Date re iewed ~ :n====~ SOP CS - Exh;bit CS. I , "vi,;o, 01 ~_ Page 53of94 G A' .O? ,.,( ;- ~.,a= §,,,*.~ ' "-1'full ~ ** "J . :~

  • Page 3(p o .*..1 ~.:

,,,,.,..,,..., Itr:** -;~ 0  :;~  !::t .: . *. Page _ _ _of _ _ _ -* L _  ; .- JJ -* _J '..._../ l 5 EnvfronmtntillTuting 5otu1ions.. Inc:. Alkalinity (SM 2320 B-1997) l Analyst \Iv I Matrix: Water, RL = 1.0 mg CaCO,IL Time initiated Ir I 05 Date analyzed 1--,.,0:-~-,.-*2-6-.\-')- - -1 Titrate samples to ===(:12==2-======== l Titra11t 11orma/ity anti multiplier tleter111i11atio11: pH=4.5 S.U. Time completed pH of Normnlity Normality (N) ofH1 SO~ pH Factor or Multiplier l Deionized water =4.5 s.u. Titrant reference number check standard number Begin ml End ml Total ml (E) = (5 ml Nn 2C03 x 0.05)/E "' 0.25/E (acccp1a blc rnngc = 0.0180 - 0.0220) = (N x 50000)/ 100 ml snmple =Nx 500 6.-+ lNRu'-t l ~~S.l'58'i o. '2.. l Lb tlll(...Wf\N.t..1 - o. - 0.'2. :.o. a oratorv co11troI slam art: I CJI l l2.'-I 12.L. o.o-i.o 5"' 1\..J.2.. Reference standnrd True value Sample Alkalinity (MV) %RS = MV / TVx 100 l number (TV) (mg CnCOJ/L) volume (ml) 100 Begin ml End ml Total ml Multiplier (mg CnCO;/L) (acccptnblc range = 90 to 110%) I NS.S\55~ 100 12-'*4  ;{~.!I Jo.z.. (b.1- (DY llY**I. (/). Dup.r1cate samp,l e prec1s1011: Snmple Alkalinity %RPO = Sample Snmplc ID volum e Begin End Total Multiplier (mg C:iC03'L) {(S

  • D) /l(S+D)/21} x I 00 l number n ~-;;i:an .SSw (ml) f UO ml M1.1a ml

~b-0 ml ~.'-f lo.z. s 35 '* .. (:icccptablc range = +/- 10%) -- .*-* ~---!~:: .... _, _,.:.";'.: .......:~ .. **!r * *: " . j Dupl icate (B) ...l ~ l4. '> 2.v( J DJ~ -O'-<"ZeJJ7 act.'1 l Mialrtx . S!Jt'k*e recoverv: Reference st:mdnrd Spike vnlue Snmplc Spike alkalinity (A) number (SY) volume Begin End Total Multiplier (mg CaC03/L) J (mg C1C03'Ll (ml) ml ml ml I ~~.SI~~ 6D \ L)O ~-0 oLL'I e.~1 ~o.z. eLJ J Sample :'lll<allnlty (B) Measured s11ilce value (MY)  % R - MY I SV x 100 (mg CnCOJIL) MY = A - B (:icccpt:tblc rnngc = 75 to 125%) l (mg CnCOJILJ J 9; '1 a, Cf ei.in. sampl e 111eas11reme11ts: Sample volume Begin EPd Total Alkalinity J Samole\Buui'ber ~ Sample ID (ml) ml ml ml Multiplier (mi;: C:iCOJ/L) Ol.t*~:n SSLiU 100 ~~.1.1 '.31. e .; .\..\ *O:i  !>5 J 19 '-1

  • ICLJ./'}

o4-t a.rtA fn Hsw I I q,1.e 1,1 ~'-l.I i *' l;.3 lo. "?I ~~ ~v( 0'1* ,q.11- 12> ~.l 14d v.o ~{ J 04* ~(/Jn(.} IY.1 ~0.1 (d.O (t1 I O'+OMn b ~o.l to. I z.. ~ll.Ol- [4 (J '::4 *8. (o.fT fl 0V:i.SvJ ~tt. 2.. 3~.o 'O. 1 C/o O.~* 'd.*b.11* (6 ..1 j 3~.o t.l4.o Q.o ' qz.. -

~,. -~ 42en Reviewed by: Date reviewed: j 6$*0\* \"'1 Page 54of94 SOP C6 - Exhibit C6. I, revision 01 13 l Page 31 Page_~_of S l Alkalinity (SM 2320 B-1997) l Analyst I Ssv Matrix: Water, RL = LO mg CaC03'L Time initiated I:=:'l!.L:.,,:::::...::======~

=: :.:01==*\=:1:===== Titrate samples to l Date analyzed 0:0 r:1trn11t norma 1t11 amI 11111/"/' f ttp. ter r.etermmnt1011:

pH= 4.5 S.U. Time completed Normality Normality (N) of H 2 SO~ l pH of pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Nn 2 COJ x 0.05)/E = (N x 50000)/ 100 ml snmple water reference stnndard ml ml ml =0.25/E "" NX 500 =4.SS.U. number number <El (:icccplnblc r.ingc = 0.0180

  • 0.0220) l ~.7.- 1Ne..~~ S,..1'$ IS"i:'l-a orn ory co11 o slam an; O.Y. 1'2..<Z 12. .4 0 .0 'Z..t'.Y2- JO.\

Reference st:rndnrd  % RS = MY I TV x 100 l number True vnlue (TV) Sample volume Begin End Totnl Multiplier Alkal ini ty (MY) (mg CaCOJ/L) (ncccplnblc range I (mg CnCOJ/L) (ml) ml ml ml = 90 to 110%)

r \JS'.:> l?S-3 100 100 }\::11.0 IDl .o 0 /.:>

1'2 . ~ 2'2..~ In o \0. \ D up,l"rcate samp.Ie prec1s1011: Sample Alkalinity %RPO = Sample Snmple iD volume Begin End Totnl Multiplier (mg CnCOJ/L) {(S

  • DJ /f{S+D)/21} x 100 number (ml) ml ml ml (ncccptnblc rnngc "': 10%)

... ' - . .:;.  :: s . ".  ;. '"'-:.: ' o;.tt-\.A- SSiv0 }II() n.i *21co.\ 3 .?, \o. \ 33 .* . ,.  ; Duplica1e (B) D ..J J_ 2.lP.\ zq."+ 3 .3 l-- 33 e;.'"' 0'5 ...1 l1 M atnx spt"ke recovery: Reference standard Spike.value Sample Spike nl knlinity (A) J number (SY) (mg CnCOJ/L) volume (ml) Begin ml End ml Totnl ml Multiplier (mg CnCOJ/L) -:}:'~SS \'33? ~loo 100 "Z.(o.. \ 3'\.~ 13.'2. \0.\ 13D J Sample all<nl n ty (B) Meas urcu spilce value (MY)  % R - MY I SV .x 100 (mg C:iCOJ/LJ MV=A-B (ncccp rablc rnngc J 33 (mg CnCOJ/L) 'i1- =15 to 125%) '1'°:J'.D 0 1c sm11p, e 111ensureme11ts: Sample volume Begin End Total Alknlinity Snmole number Sample ID (ml) ml ml ml Multio lier (mg CaCOJ/L) 06.t)2..r:t m\+.::vJ \O'O ~<i.~ IJ..1.5.'S w. '2. \0.\ lq3 o<;.o~n I o.o \.o \ *~'"" 1,,0 0505.11- ~ . ~ In\> \'2...o lo.o \, \ -I £ .\ -*lJ,- \I A -;,\..\."lb* Cl f> rnHsw I uv \ 2- lOi> ....~s - ......- ...... 2.4-.'l ~1.1 ...... - \*b ~ l.lP\.C: L~) 1,.3 ~ V1 I L,,4-os.-cn..-\\ J 3 _..... '2.1. 2. -~~:; Lt. I v'2 ' , Q$c) \.<fl-t\ oS<T':> .~ W.:feltu~ I I ~v .~1-~ '41> .1 3.1+ ..,, ' l o'l

2. I ~-'1- L14.() ~ °' 1_'\. ~-::+-

n oc; as . l \o ~ 3 I J_ l.lP ~R 3.\ '.'I.) ---- t-3 oi::..o~n* Reviewed by: Date reviewed: I O~* tn-r1 Page 55 of 94 of- (v*nrt-19 vo1~mt t(... SOP C6 - Exhibit C6. l, revision O1-01-13 l Page 3 <i Page ~ of ~-- 5 l Alkalinity (SM 2320 B-1997) l Analyst! ~ ~ Matrix: Water, RL = 1.0 mg CaC03/L Time initiated FS f~Pt Date analyzed DS:;;-;r

  • ij Titrate samples to Time completed pH=4.S S.U.

T:1frm1111ormar1tv mu,I 11111I. ltp r1er tl etermma/1011: pliOT- r - - Normality Normality (N) of Hlso~ pH Facto r or Mu ltiplier l Deionized water Titrnnt reference r--.. ~ .. st:rndard Rea in ml End "" Total -- _, = (5 ml Na 2C03

  • 0.05)/E

= 0.25/E = (N x 50000 )/ 100 ml samp le = Nx500 =4.5 s.u. number number (E) Cnccc"}1t:tble.i;ane:c = 0.0180 - 0.0220) l Lb a oratory co11tr o sl am l a n: I ...;..0501o<T Reference standard True value Sample Alkalinity (MVY  % RS = MV I TV x I 00 number (TV) volume Begin End Tota l Multiplier {mg CaCOJJ'L) (acccplnblc rnngc (mg CnCQ3/L) (ml) ml ml ml .. 90 to 110%) J~S \SS~ 100 100 -"?-. -=r- l~'l> 10 .1 JO .\ l0'2.. 102.-, 0 Oto D 11prt cale samvl e p r ecrs1011: Sample Allmli nity %RPD= Sample Samp le ID volume Begin End Total Multiplier (mg CnCO.i/L) l(S - 0) /((S+0)/21} x 100 number (ml) ml ml ml (n~ccptab l e range c :f: 10%) 1* *-:..-*

  • s *""[!"'-, * ,. ""~ :; *r ~ ..... *

... l-"1 O.SO\.0' 8"""eMu f-1 lY"\ l to o 1 4.~ l~'l c~ 9 1n. ) 3'1 ..  ; :: .";. " _;.* -~,*.:* ' ~ Duplica1e (B) J_ 1'il*r "2.'Z-.v 3 .'\ .l. D 3q B~C QS.O ..H:J utnx spL e recovery: *1 I Reference stnndard Spil<ev:iluc Sample Spike allcnlinity (A) I number (SY) volume Begin End Total Multiplier (mgCnCOJIL) (me. C:iCOJi'L) (ml) ml ml ml 5o JOD 1i.-r *).1::\- 9.o l o.I '11 Snmple alkali nity (B) Me:1s ul'ed spike v:ilue (MY)  % R - MY I SY x 100 (mg CnC03'L) MV=A-B (acceptable rnngc (me: CaCOJIL) .. 75 to 125%) 3q 52 tc 't. o l>j o S ampIe m eas11re111e11ts: r Sample volume Begin End Total A lkalinity .I Samole number Sam11 le ID (ml) ml ml ml Mu ltiplier (me. CaCO.i!Ll \\05.lfu. ~~ -:rt.Hv fun 2- 100  ::l"l .1- ;ln _R 3. \ fU.\ ~I J t\cS.C'S.. \C:..

11. 0S01.. o..f Ao.1 ,.D J

l .3 _L 'JC::. &>.'J 5+.o 4.1.Q .:SS. I ~"2...

1. 1 l/4) 32

.w.u. J \1 oSo'-! .ol r 2.. I _L I ~.I ~(,.'4- 1.3  !< 4) 52 \ I b C:..Q\o. b\ l 3 ~-.<.\. ~'l,\o J.'L (.o+.) .t.\.'b n oscn.- -4 1 T"tc_ 'J _ ()(")\ A-m ~s  :.>>':r.v ~!'5 2.ct LA'\ . 1"2.0- l\t noS(ll... \S ~, ..;r Ji.ilS ~ .... s.1. 114) 130 * \2C\ \\ OS.<:12.*n_ PN\ .Y.~.1- Mto.1,1 2..q 'r...i.'\ \~ -\ \1 1.1 lJ~<Sl. ' 0 ~ ftn 1$ Q ........ o.o 8. 1 3 .\ *~, -- \-3-,.,... .., -z...o - 1z:; 110.* ... ~ * .... Reviewed by: Date reviewed: J 0 s.- 1)1 * .-1 Page 56of94 SOP C6 -Exhibit C6.1 , revision 01-01-13 l 0 ~ ;.~ c* y* . . , * "' * ~- . ~~,.* .Ff!G~.J ry;..-- - ~:, r: ~- ~~- - Page 39 '-,) LSC *..) * .*' *: .~ *i"1 '~-:... .*~iI ,. _ .). ' 'i Page 3 of_5__ - - t.___../ ) . Envlronmrnlitl ~ttng Solu\ion s. Inc. Alkalinity (SM 2320 B-1997) Analyst I & Matrix: Water, RL = 1.0 mg CaC03/L Time initiated I':........,_ ~17 l Date aaa lyzed ===~:1::'-':.a:r;}:*=O-===== Titrate samples to pH=4.5 S.U. Time completed ~ r:1lra11t 11ormar1ty am mu l ftp ' /'rer'l etermma/1011: .. ,. Normality (N) ofH1SO~ l .--- Deionized water Titrant reference Normality cnci:" standard R .. arn ml End m1 To fa I (5 mJ N11 1 C0 3 x 0.05)/E 0.25/E pH Factor or Multiplier (Nx 50000)/ 100 ml sample - Nx 500 - 4.5 s.u. number number (E) (ncccplnb1c r: n 1'1110

  • 0.0220)

- .. l ,~ Lb a oratory coutroI stmul artI: l Reference standard number True value (TV) (mg CaCO.i/L} Sample volume (ml) Begin ml End ml Total ml Multiplier Alkalinity (MV) (mgCnCO.v'L)  % RS = MY I TV x 100 (acccplnblc range = 90to110%} 100 100 \oG.. \02.. 01:>~ "ft::PS \S'S :S 3 .\ 13.1.- j'D.\ Jo.I D up,/'1cate samp I e preCJs1011: Snmple Alknlinity %RPD = Sample Sample ID volume Begin End Totnl Multiplier (mg C:iCO.YL) {(S

  • 0) /((S+0)/21} x 100 number 1V'A (ml) ml ml ml (ncccplnblc range ~ +/- 10%)

.. *. .. . ..... ' .~;-v50 s ~.*'i: \1 Cf::lS\.1.f f\t.-F OCl *~ 13-'2- 21.0 -=1.'if 'l.) In.I l\oD -\'>> ; . -** *-- . *-* j_ D l Duplicate (B)

2. J.o 2.c::r.o ~.o Li.) ..\_ lltO ~ 06 °0"1-!1-atrtx S/Jl e recovery:

Reference st:tndard Spike value Sample Spilce allcnlinity (A) J number (SV) (mgC:iCO.i/Ll volume (ml) Begin ml End ml Tot11I ml Multiplier (mg CaCO.YL) 100 ~\..lSS lSS3 ~a; ... 50 2.)."> ~ 13.0 :z.) 10.\ '2..1..e c I -.... ,.,.,.. Snmple allrnlinity (B) Measured spike valu e (MY)  % R "" MY I SV x 100 (mg CnCOJ/L) MV = A-B (acccptnblc rnngc (me CnCO.i/L) =75 to 125%) _J llPO \OC \eo.c"'/o

2;,., ""'""') *"

snmp.I e 111eas11reme11ts: J Sample number Samole ID Sample volume (ml) Begin ml End ml Total ml Multiplier Allcnlinity (m!! CnCO.VL) r1 O.S\Th * ~o -fJAAt r- vp 5o 3~.'5 ~"I( . ~ ).~ 'ti..') \'C). \ 3<.. J \10~61.'\ l~O?J.20 j I Ni I 6'X.~ L..IJ,I.\ S.K ,.,_-., , l'Z \It rnM1w 2.5 l),Q 3 .'1 .~.q II.+) \loo ,, a. J \' c.s. o-i- <lS QN. c-n ho c.U. \1 oSo\.~ --r.JA~N \O\ \ So .3 .'I +.t> -:}.o 'l.1 ~\ ~!=! ll1. \ L'L\ , ~ 6ct OS*~ ~I ~~.";f; I 2. -=i.q 13.0 3 ,\ I">,'\ k '2, \4C\So'S. \\ J 21 13*0 flD .3 .'l, .~ (,-i.-) ~-:r '1.Jlt S'Qr-> 'Q I 1,.JT ~ '5G\ o-s~ J 1\0So\.ti.J. \\ 0 S.<P.>*1>\o ~ 2 l -- Reviewed by: llt*"i 1q:i 14..1 O:Z~.5 l) 3 .~ .'l (;).'\ c.,,...\ - Vt Date reviewed: j ~c_, -01* \" Page 57 of94 SOP C6 - Exhibit C6. l, revision 01-01-13 ~~~~~~~~~~~~~~~~ ~~ ~ l Page ~o Page  !.-{ of - Alkalinity (SM 2320 B-1997) Matrix: Water, RL = 1.0 mg CaCOJ/L Analyst j f?Su Time initiated i-:::.--.._~"T Date analyzed 0~6:-~1:>_~---~*-\'1-~-:_-_-i~ Titrate samples to Time completed ..__ I ______ _, l ._ f-_- _... pH=4.5 S.U. Titraut normality and multiplier tfeter111i11atio11: ~ .,_ . Pu v. Norm3lity Normality (N) of H 2 SO~ pH Factor or Multiplier l Deionized water Titrnnt reference check st:ind:ird --ml.. "' ml Total lnr- ~5/E (5 m l Na 2C0 3 x 0.05)/E (N x 50000)/ 100 ml sample =Nx500 = 4.5 s.u. l number number (E) ( p b --- =0.0180 - 0.0220) .. u~tol\ Lba oratory contra I Sant t Iart: I Reference stand:ird True vnfuc Snmple Alkalinity (MY)  % RS = MY I TV x 100 l number (TV) (mg CnC03/L) volume (ml) Begin ml End ml Tot:il ml Multiplier SS\- 1:15-o~ *l ~ (mg CnC03/L) (ncccplnblc r:ingc =90 to110%) 100 100

r~ 1~5:, ~~.? =32 IT c:i.q 11.,.k tro. I I t>t> I t;j;;:,.o0 / ..

D up,rtcate Sffl11fJI e prec1s1011: Sample Alk:ilinity %RPD = Sample Sample lD volume Begin End Total Multiplier (mg CnC03/L) ((S

  • D) /f(S+D)/2)} x 100 number '&../IA (ml) ml ml ml (ncccplnblc range * : JO%)

- . - . ,. . **.: . *~ ... *~.....*.*t*.'"*-;:: .. ~N ID I 1~'2. s .  : ** ~ .t ' no$ o~-'.t 5't> 32..4 35.'P 3'2.. t'.l-\ 10.\ lO 5o 35.v Hg.l. '0.'2.. ~l \0.1 ~ 110 Sample allm linity (B) Mensured spil<e v:iJue (MY) %R=MV/SV.1.JOO (mg CnC03'L) MV = A - B (:icccptablc rnngc (mg CoCO.VL) = 75 to 125%) .1 (;5 nr IOS' Ori:' " Of~ - I05'JI, samp, e 111ens11re111e11ts: o~n I .. .J Samp le number Sample JD Sample volume (ml) Begin ml End ml Tot:il ml Multiplier Alkalinity (m2CnCO.i!Ll \10 SQ\.(C) ~~A ~ t0 l01 Uv \ So (),0 2. .'1 2 .'l ,.,_, IC.\ ~5C\~ _J l "l OS'S?> :6 I 2. 2. ~ Lr.I ~ :2..  !-?\ . ~5 J 3 't.c; J \'"\o<":ioS. \1 \\DS C\ .64 l..J ASG..-NlCl \ >Jl\l\J \ le.I C'+ J.:'55' 11:-? ..t'a5 ,.... 3.c+ .,3 .o U.\ - "" ,.. , ~~'l (t>Cf l4A lt I \-\OS~.~t... z_ lo5 pS*~'1-A-I ......... __ ,.,, .;;>1.1 4+.-5_ 3.i..os ('2.\ noS0'5. l~ ~ 3 -- ~I."'.} 3.-1. D-1 \g~ J \ \ OSIR..-OS ~\le \ 2.- q.S .;J.S.o 11.5 z..o L?o) I ooC Y\ oSO~.Q"'\- I '2.- l 11.c:;' 15.lo .9..\ fc..,") 1\00 I  ! I _...; \\ ~s1:11o.01... 3 J- 13.~ 15.S ).g I c:T>\--- ~&L ""'*"' **~ '\Vo Reviewed by: Date reviewed: I O!.* en . 1 -\J Page 58of94 SOP C6 - Exhibit C6. l, revision 01-01-1 3 l Page '4-1 Page 5 of 5 --- Alkalinity (SM 2320 B-1997) l . Matrix: Water, RL = 1.0 mg CaC03/L I Analyst Sc:iv Date analyzed 1--o"'""s-.-o-" '.}-*-lt----1 Titrate samples to .Time initiated ~~1:r Time completed .__ r---- _______, l Titrant 11ormalit mul 11111/ti lier delermi11afio11: pH=4.S S.U. Normality (N) of H 2SOJ pH Fnctor or Multiplier Deionized Titrant = (5 ml Na 2C0 3 x 0.05)/E = (N x 50000)1100 ml sample water reference =0.25/E =Nx500 = 4.5 S.U. number number range

  • 0.0180 - 0.0220) l L a boratory controI slaml artI :

Reference standard True value Sample All<alinity (MV) %RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier (mg CaC03'L) (ncccptnblc range (mg CnCOJIL) (ml) ml ml ml = 90to110%) 100 100 11> .o \o l. o 0 -f~ ISS-3 ~5. o 35.o fo.1 IOI (o Dup,/'tcate samp,I e prec1s1011: Sample Allcnlinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaC03'L) {(S - 0) /((S+D)/21) x 100 l number 1I Q.507.. ok:> 1::;.r\/1.1 l hlln.cJ \ (ml)

1. 35,0 ml ml 4J.'+

ml lt .'-+ t<n\IO.l s 3z.oO (acceptable r:ingc =  ;. *** * .;- --~:.: _..., _- ... .....

1: 10%)

**~ -~ Dup!Matc (B) D l ..!- o.o ~.c; (o~ I *o\ _l 33oO -Ir. 'l:l.o!. .. ~ . 1°1

  • f>.<;'... ~ . .~ )n...

.,, atnx sp1 <e recovery: Reference stnndnrd Spike vnlue Sample Spike alknlinity (A) number (SV) vo lum e Begin End Totnl Multiplier (mg CnC03/L) (me. CnC03'L) (ml) ml ml ml 'f }-.!SS IS'~? .::<5oo  ?._ o.o 11.S 11.'3 ~) \t:>.I 5l?oo Snmple allrnlinity (B) Measured spike vnlue (MV)  % R= MV I SY x 100 (mg CnCOJ/L} MV .. A-B (ncccptnblc rnngc (me. CnC03'L) = 75 to 125%} J 3300  :<5oo joO .C) 0 /o samp,le 111ens11re111e11ts: J Snmple number Sample ID Sample volume (ml) Begin ml End ml Total ml Multiplier Alkalinity (mg C:iC03'L) '\\ cso~. <[) Cw~J .., hOAd 2- '.2. II.$' 11.~ lD.4 I~'\ )O. \ ~?C>n l 1\osot...o'-l n os01...<J1 PA~a.1 j o4c."'\L \ 3 r:i.q ..71-l..2, ..'.ic..l.~ 2~.3 II.'+ '+.o ~\ 3-z-.oo 1~1.')I.') . <=io' noS£1-t.<e> I 2. 2.S.'; .~2 .-? 4 .'0 ~o\ '2,Qoo J \ \ O'S.D\...Cf.;> .l 3 32.~ 3t...~ 1-\..o F\'()) -Z.Ooo 06,0t.{.1'} .5~5v) I oO ~.3 t.!U.S lo .'2. -~ IC><:> - S1 L 'b/0-o 1~']- Reviewed by: Date reviewed: OS-CJ)- 11I Page 59 of94 SOP C6 - Exhibit C6.l, revision 01-01-1 3 l ~ l~ffil ~ ~}:fl rfPJ '~ Page } ,-

t *r-- f *.

I  ; ..._ I '~ Page _ _l_of 6 ~ c_-:,./ l _; \------- _J *-

  • Env!ronmtnUI Tasdng Solutlons. Inc.

Hardness (SM 2340 C-1997) l Analyst Ilu RL = 1.0 mg CaCO~/L Time initiated ~~IU:"""l_.UJA . . .-=<.P.___ _---1 Date analyzed ._0_'1_*~-6-.-,1----1 l 1 ran t uorma 11y antI mul tip, r . r - 1er tl eI ermma t011: Time completed ._...._Q""'-6l.P""'-=------' Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier l reference number standard number ml ml ml (E) ID~o = 0.2JE (:icccpfnblc rnngc = 0.0180 - 0.0220) = (N x 50000)/ 50 ml sa mpl e =Nx 1000 z_o.o 1.tJq,.--=tQ, I NsSI 1-Nl4 l).() \O.o 0.0200 l L a b oratory contro I stamInn: Reference standard I True vnlue Sample Hnrdness (MV) %RS=MV/TVx 100 number (TY) volume Begin End Totnl Multiplier (mgC:iCO.v'L) (:icccpr:iblc rnngc (mg C:iC03/L) (ml) ml ml ml = 90 to 110%) \ N$S 1'-1-ef} 40 50 (D.0 \2_o -z_o -w.o '-lo \00 01. Dup.r1cate samp,Ie prec1s1011: Snmple Hnrdness %RPD = Sample Snmple ID volume Begin End Totnl Multiplier (mg C:1CQ3/L) { S - D} /f{S+D)/2J) x IOO number (ml) ml ml ml l Q4*80. /). .! .Ssw Duplicnte (B) Ob ~ l2.o jvlo l'-t.o 1~~0 '2.,o -Z~o Zo.o j s D YO 40 .. --41-.0 '-I 2e n- **'

Matnx sp1"Ii*e recovery; Reference standnrd Spike vnluc Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CnCOJIL) (mg CnCOJIL) (ml) ml ml ml \JllS.S1'ltH ~o 6D 14.u \>,u ~to zo.o eo Sample hnrclncss (B) Measured spike vnluc (MV)  % R = MV I SV x 100 _I (n1g CnC:OJ/I.) MV = A-B (ncccptnblc rn11gc (mg CnCO.v'L) = 75 to 125%} I samp.le 111eas11re111e11ts: '--10 yo 100. in. Sample volume Begin End Total Hardness Snmole number Snmole ID (ml) ml ml ml MultioJier (mg CnCOJ/L) J TV =ND ,;~ lA I. Blank (should be = 0 111£ CaCO.;/L) 5D l).Q b.O o.o w.o ~ r O'-{ :'A~'}. 6E>W 'e:>.c> 2,).() "2.,o '-JO J O"l.11a .n mi+sw ~Q,O "'4.ltJ 4.1.tJ Cl2.. b Y.14.11-A ~tild J.9./ '{.-;- C/o l')'{.j q.1}8 iA'1.J j.3.-\ 4.~ 66 O\,/.JltJ.11 A "3.J. '.) ~.'1 J.y ~ J 01../ *9.0.11 r.... ' ~fl '-41.c - t../.1 ez.. bv <lid~- (-} 4W '-4i.8 ~.e I (.ti I v {b 41.e; 43~ O.i.o (2 l J (.., 43.4 44:0 O~b ' 12-Note: If >15ml oftitranl is used, sample must be diluted. Page 60of94 Reviewed by: Date reviewed I 6S *o\* t'\ SOP C7 -Exhibit C7.I. revision 01 -0 1-1 3 l Page -2.- - Page - - - - --- 2- of 3 l Hardness (SM 2340 C-1997) l Analyst I Lf'._, RL = 1.0 mg CaC03 /L Time initiated E ---i 01.t*2-(9p. Date analyzed 1--Q=-~-=-.- a-6-.l-}----1 Time completed ~ l r:11 ra11 t 11or111a uy a11t u* r 11111 tp, 1er tlet ermma /'t011: . Htr..fillf Normality check Begin End Tota l Norma lity (N) ofEDTA pH Factor or Mult iplier l reference number standard number "" I .1 (E) (:tcccptnb1e 0.2/E rm.,.~ u*U*~..ll22Dl =(N 50000)/ 50 ml sa mpl e =Nx 1000 v-l L aboratory controI slaml an: Reference standard I True value Snmple Hardness (MV)  % RS =MV/TVx 100 number (TV) volume Begin End Totnl Multiplier (mg CnCOJIL) (n cccptnblc range l 1~ \'-it)"} (mg C:iC03/L) 40 (ml) 50 ml 4~.(J ml c..\"10 ml z_o w.0 YO = 90 to 110%) l c:xJ. ({), Dup.t:1cate sm,,-o.l e prec1s1011: Sample Hardness %RPD= S ample Snmple ID volume Begin End Totnl Multiplier (mg CnCOJ/L) {(S- 0) /((S+D)/21} x 100 number (ml) ml ml ml l Q\)Ot)Jn Duplicate (JU D 60 .J 13.1 IY.1 15.1 1.0 1.0 -io.o 1 s 't<J D .. .. --1.1 o\..\z.e~ l<..\. t 't-<) l atnx spi/;<e recoveri1: R eference st:mdard Spike vnlue Sample Spike hnrdness (A) n umb<?r (SV) volume B<?gin End Total Multiplier {mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml \ }.)~l'/fH- '4D 6U \ ll.I l1-o 2. .t1 'ZD-0 66 I Snmpl<? hnrdncss ( B) (mg CnCOJIL) Mc.1surcd spike value (MV) MV=A-B (mg CaC03/L)  % R = MV I SV x 100 (accc pcnblc range = 75 to 125%) -Z-0 35 QS.07, .I samp.l e measurements: Sample volume Begin End Totnl Hardness S:imple number Sample ID (ml) ml ml ml Mul ti pli er (mg CaC01/ L) J T V=Nn Blank (ShOUIU uc u 1111!, \..llL.'-'.Yt..J ~A. 0 t1:uJ1r &.\f~f-r- i; 5D 17,0 11*!1 o.ti l,b.o 1e I I 1 0 I=" 11-.'l ll1.3 I.~ Z.8 ~ Ill .., 2J. / 1,6 3<.o J 1-{ a. t I a~.-=1 I. It) 32 -r Ot~.+ a'u J.1..f 28 J - I tJ. ~.I ~1S b. y "1B K ?i:J-S 3JS ~* O Bb L ol:S 33.~ 1.1 3e

  • M I . ~~.~ JJ.tt O,') JO Note: If >15ml oft itrnnt is used, sample must be diluted.

Page61of94 Reviewed by: ~~--- \t\~ ~--~~ Date reviewed I oS*O\*\\ SOP C7 - Exhibit C7. I, revision 01 I3 Page _.3_ _ Page 3 of_...;:3'--- l Hardness (SM 2340 C-1997) l Analyst I vl_; RL = l .0 mg CaC03 /L Time initiated , .........._ Nl-e!lf-Date analyzed t---O"-l.{---.- .1-5-.-j }----1 Time completed ~ l r:11 ran I norma nt 'V antI muIrt/J.r1er t11 e ermma . r1011: ~ Titrant Normality check Begin End Total Normnlity (N) ofEDTA pH Fnctor or Multiplier l re1,... number stnndnrd number ml ml ml (F.\ 0.2/E (ncccpt:t blc range 0.0180. 0.0220) = (N x 50000)/ SO ml sample -Nx 1000 -~<H70~ l Laboratory co111rol stmulnrtf: Reference stnndard True vnlue Sample Hardness (MY)  % RS = MY I TV x I 00 number (TV) volume Begin End Totn l Multiplier (mgCnCO,>/L) (ncccptnblc range 1 I JJSSM6} (mg CnCOJ/L) 40 (ml) 50 a3.1 ml ml .75~ 2..0 ml ZQO 40 =90to110%) I DO, l/J* Dup,r1cate sample nrec1s1011: l Snmple Snmple JD Si1mple volume Begin End Totnl Multiplier Hardness (mg CnCOJfL) %RPO= {CS* D) /f(S+D)/2(} x 100 number (ml) ml ml ml l .* ,. d) lJ ll/lJ.._,.. 1\J 5D 26.'\ :j7'.':l 1.6 2.0... 0 s .3 (o  :.. .. ~ *~ Duplicate (Bl) j 11.r 3q, S" i.e j D 3<.:? -o42eH atnx sot <e recovery: Reference standnrd Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CnCOJfL) (meC:tCO.>!Ll (ml) ml ml ml ( >J~N-~:r 4u 6U 3r."} c.tf .<<; ~. 6 w.o 70  % R = MY I SV x 100 J Sample hnrdness (B) (mg CnCO,>/L) Measured spilce value (MY) MV=A-B (:1cccptnblc rnngc = 75to125%) (mg CnCOJIL) '3cp 40 100.d>. .J Samp.I e 111ens11re111e11ts: Sample volume Begin End Total Hnrdncss Snmole number Sample ID (ml) ml ml ml Mu ltiplier (mg C:iCOJIL) J TV l'IU "Rl::ink (should be OmgCnCO.;/Ll * {\I.{ 2:t31) OvW\h.. _ 0 6D ao.'$" ~1.4 Q .C\ a,o,,o /8 J 0 p I o,~ 12- ~'*" ~Q.u ~ - () aQ.o b,!J..9- o.~ ~*b O.f. 14 J fl. a!M* ~R4 s ~~.l} ~3.'- o.:, ltJ .O -(" J..o.J. J..4.o o.<1 Iv J v 1 ~N.o ~-;.~ I.~ :20 \) ~c::; ~ ~~.c; b. I L*D w I ~s.~ 3,5.7 o:i.. ;i~ *~ 'ilM .'T"I I. I . ti.a us~d. sample must bc~ilutcd. ) /7 Note: If >15ml oftitrant is Reviewed by: f' A I Date reviewed J o~*t>\ .n Page 62of94 .... SOP C7 - Exhibit C7. I, revision 01 1 3 l 0 0 0 [§~:; .t

.

-;-, ,_ ~ #!a *;:J r-1 .... ~-* .jj-C::J ..~......._, Page 4 ---  ? ~ c:-~;~ Page - -\ -of LJ. l j * ~) Environ men Ial Tutln!J SoluUons. Inc. Hardness (SM 2340 C-1997) l Analyst I II-' ) '?t>l.- RL = 1.0 mg CaCO;IL Time initiated ~\"""J.;_~ .;;__(J?.:.----~ OG.~.n-l Date analyzed r tr 1 rmt ( 11orma tfJ1amI mu 1pCrer tI eter111111at1ou: Time completed ........_l-'4-'--"3:-'-----1 '-l. Titr:int Normality check Begin End Total Normality (N) of EDTA pH Fnctor or Multiplier l reference number standard number ml ml ml (E) = 0.2/E {ncccplablc range = 0.0180 - 0.0220) = (N x 50000)/ 50 ml samp le = Nx 1000

r~e.1~ :r~ 1~4-\o 0.-0 \0.0 10.0 n O? ()0 Zo.<>

l l (1 borntory Reference s tandar d co11tro I slamI an: I True value Sample Hardness (MV) %RS = MV/TVx 100 number (TV) volume Begin End Total Multiplier (mg CnCO.VL} (ncccpt:tblc range l r 'N$ l'-\<t-:r {mg CnCO.i/L} 40 (ml ) 50 ml 10.0 ml I\. 'i ml I. q; 2-0.o 3\C'.C =90toll0%) ~ .cc.lo Dup /"1cate snmp.I e prec1s1ou: Sample Hardness %RPO = Sa mple Sample ID volume Begin End Totnl Multiplier (mg C:tCOJIL} {(S - D} /l(S+D)/21} x 100 number (ml} ml ml ml s .. 05.cH.(} 6Sw c;-o \).~ 1~ 1-"1 'Zo.o 3i . *-: .. .. Ouplicalc (B) D 4 _L ).'\ .L 3~ ---£'!1<..- os; -*.,..-4 \~. "+- l'ii.~ atrix s11i <e recovery: Reference standnrd Spike value Snmple Spike hnrdness (A) number (SV) volum e Begin End Totnl Multiplier {mg C:tCO.i/LJ (mg CaCOJ/L) (ml) ml ml ml -rLI£.<. ~ +- >-ID So l~."t r;r.w .a.q -io.o -a _.I Sample hardness (B) Measured spil<e value (MV) %R = MV/SVx 100 (mg CnCOJIL) MV=A-B {ntccptn blc rn ngc (mg CnCOJIL) = 75to125%) es. <>5-0.....y 38 1.f-'40 )OO.o 0 Jo __] samp,l e measu rem ents: Sample volume Begin End Total Hardness J Snmnle number T V = ND Blank SamulerD (should be "' 0 mg CaCO;/L) 5o (ml} o.o ml D.o ml o.o ml Multin lier -zo.o (mgCnCO.i/L) ....w (6.a;i .11- ;YI ltSW ~.~ .2&.\..\A 1-j. ~ ~ J t'JS.~./)- I '2&+."' 2~.'1- '-\-. I ~ ()6.0S,1). .J z.g,'} ~~.\ '-\..tl. B'O _J -u..-,*H\ (n H- ~ v..) v egg l\ '1. - ' ~ .\ 3'}.'5 \}., i-\. !-\- 0'-\*'1.b-\\ & I 2 31-.5" Y.\_~ .t.+,g 't;\p j 3 J O~*<n..*fl \\Qg)\.0 \ .:je,l.l R.l5ot'"\ ) A. I.~ D.O ~-' 2 .'l ...... ~

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~"' si \ .:-- ...~.:!>~ \ 2.. ?q &:\ I. ~."} SY. J nf'\~ os. *S J 3 -- ~-~ Si!.~ 2 .~ _l-- Fl.V.* Note: If > 15ml oftitrant is used, sample must be diluted. Reviewed by: Date reviewed j O~*() \*1'1 Page 63of94 SOP C7 - Exl1ibit C7.I, revision 01-0 J. IJ l ~

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I .!'*~ . -:---_ .. Page Page 2... of 4 S ~-- l .... - I '- . . _., * .._) Environmcnta1 Totln_g SofuUons:, Inc. Hardness (SM 2340 C-1997) l Analyst I O'O*Ovs~(... ~ RL = 1.0 mg CaCO,IL Time initiated I"-- OSD"'~ --i Date analyzed "\ Time completed . ~ _ l Ti'ttrnnt 11ormaIity anti 11111,. /tfJ,rter llelermma/1011: ... --- . J. . a........uu. litv check Begin Normality (N) of EDTA End Totrll pH Fnctor or MultipJicr ,, l reference number stnndnrd number ml f--41?.l ml = 0.2/E (acceptable range= 0.0 l80 - 0.0220) = (N x 50000)/ SO ml sample =N x 1000 own-- l ~ Lb a oraforp con I ro I s I flll~Iarl: I Reference st:indnrd True value Snmptc Hnrdness (MV)  % RS = MV /TV x 100 number (TV) volume Begin End Totnl Multiplier (mg CnCO.i!L) (ncccp tnblc range l :f-..J~ \ '--\-'&1-(mg CnCOJ>'L) 40 (ml) 50 ml l:O.\.\- ml Jv.4 ml 2..o 2J;> . o >+o = 90 to 110%) I cc.o 0 b Dup.rtcate smm1.Ie prec1s1011: Snmple Hardness %RPD= Snmple S:implc ID volume Begin End Total Multiplier (mg CaCOJ/L) ((S- D) /((S+D)t21} x 100 number (ml ) ml ml ml s -* .. *. .. \'"lb SQ\ *6 'l- w;:r-~~' 5o 10.4 114-.q 1-\-.5 1..o.o 'lo '. . I .. l-Duplicate (B) j_ IL\- ."I l'lH- ~s .1-D 'lo --as, 05'.*..,,,_ afrrx sp1 re recovery: Reference stnndnrd Spike vn luc Sample Spike h nrdncs s {A) number (SV) volume Begin End Total Multiplier (mg CnCO,tL) (mg CnCOJ/L) (ml) ml ml ml IN5 s;,'-ten '-to So li+."1 2..1.'+ "'.'5 'Z,o. ..-:i \60 Snmple hnrdncss (B) Measured spike value (MV)  % R = MY I SY x JOO (mg CnCO.i/L) MY=A - B (ncccpl:tblc rnngc (mg CnC03/L) =75to125%) 'lo J-fC 100.0°/o J SampI e mens1treme11ts: Sample volume Begin End Totnl Hnrdn css Sample number Sample ID (ml) ml ml ml Multiplier (mg CnCO.i/Ll J TV=Mn Blank -61Xt1rt-(SllOlllO OC V Oil! 1...aLU,;ILJ ~ l\0'5.~.~~ w-~~ t.. ".>"... .,""*or. ~"i 2..l '4- 25.4. 1-1-.o -z_o.o '60 J \\~~OS l.b j 3 ....L 1~.i+ ,2o,Q >-h1a q~ \"'"\~en.. e>--4 . llr.n ~ ,, 2 \ C::..""1 3o.o ,,., .:2. '} :.:t Ut_ J 2.1! ~'o ' i ().S.C.'\. () \ ' '1.C I I '2.?.1 11oso1o.o\ J 3 J_ -~.) ;1. '1 ~.4- ~'B \.., oScn.

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Page 64 of 94 Reviewed by: ..___ __ ()'. . . .~ rt Date reviewed I O.S* ()'- .. (\ SOPC7- ExhibitC7.l,revision01-0l-13 l Page ~ Page 6 of Y.. l Hardness (SM 2340 C-1997) l Analyst I Sc:it..- ,;v RL = 1.0 mg CaC01/L Time initiated,............._ O~I~ Date analyzed OCQ. ou. )'"} Time completed 'S TiU:o.11t uormalitv aml 11111/tip/ier tletermi11ntio11: Titrnnt Normality enc ... " "' 'h End Total Normn lity (N) ofEDTA pH Fnctor or Multiplier l - 0 reference standard ml ml llll 0.2/E (Nx 50000)/ 50 ml snmple number number (E} (acccptnblc range = u.u.;;_ n n??O\ .,~ Nx JOOO - ........ f ... l L a borntori1 co11tro I stnmIarl: Reference standard 1 True value Sample Hardness (MV)  % RS= MV I TV x 100 number (TV} volume Ile.g in End Total Multiplier (mg C:iCOJIL) (ncccptnblc range l :r~s.s t~~1-(mg CnCOJ/L) 40 {ml) 50 ml 1&.\-.\ ml \\#.0 1.q ml 1,,o.o 3'B = 90to110%) Cf'5.c 0 (, Dup.rrcate snmp,l e prec1s1011: l Sample Sample ID Sample volume Ile gin End Total Multiplier Hardness (mg CnCOJIL) %RPO = {IS- D) /J(S+ D)/21} x 100 number -r.JA (ml) ml ml ml .. J \""\t.>SU'\-1.K ALF O'.J1 ~s llf,O 1.\.1 5.1- ,,.,'?A>.o ~~ 1- '2.ci ,,~ *o.. '. D..,,..,.,. .. ~ Duplicate (B) J_ .l) .-:r 'A-:-S'" 5.Z h.'\~ I~ f:6v '2.?)2* .,,z:~.., +.==f-es" 05.01.*11-Mntnx sp1.,,<e recoverv: Reference standard Spike value Sample Spike hardness (A) number (SY) volume Begin End Total Multiplier (mg C:iCOJ/L) (mg CnC03/L) (ml) ml ml ml

f\.JSS I..\.~'i- '?o 2.S -l'..!*~

. 2qs 2.0 ,, ":\ Z,O;\:> ~.,...~ .., 310 -* ... l Snmple hnrdncss (B) (mg CnCOJIL) Mc:tsurcd spilce va lue (MV) MV=A-B (ml! CnCOJIL)  % R =- MV I SV x 100 (ncccptable rnngc =75to125% ) 2.30 ~,,,.,. oS* tbl'l- 'OD \O\>. 0 I:)~ J samp.Ie 111eus11reme11ts: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mgCnCO.i/L) J Blank

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-hi') 1?1 o 50 ez, J 11'f>C(o4.oS (' N...rn ~,,; _ _,, "S.olt'\~ 31n.1. IHO.C\ ""'*\ ~ A,.... 0.. -' ~ f vTU =-->-. l IU \ I ...... a --~ l,'T l 'I I ~'° . ,:'\ ~ ~ ' - ..... l I ............... - r ... "I lo\D ., ' . lD'-+ I). " '\ "- I I .. ~ I '"T0.'1 "'?.'l.V I* "I . . I 0..\- \1 c.c:.o \ .o-4 lv'A~ ... \101 11.li \ f)o ,..,,..., 3.'2.- ll,.7- la'+ \1 o~ o:.. '!>lo j 2 Note: If >151111 oftitranl is used, sample must be diluted . _L Reviewed by:  ;.:z. (i.? .3.~ -- Date reviewed I I.Iv o ~*u\ji*\ 1 ~*~l..4!t:i a;~ CT\-\"'\ d{ SOP C7 - Exhibit C7. I, revision 01-0 l -1 J -=1- ~~ -~~ff!! Page ~ :. ~  :"l '<.:\ ' j Page 1+/- of ~ -' L_ - I ...___./  ;- -* , -~- l ) Envlronmen1~ITu1ingSoluOons. ln~ Hardness (SM 2340 C-1997) l Analyst I SSv lfJ RL = 1.0 mg CaC03/L Time initiated 1~ O.sot#C1-1 Date analyzed 05 .~* l'3' Time completed "'4F Titrn11t 11or111n/ity mul 11111/tiplier l I etermimrtiou: --++<<---* "'-,rmnlitv check Begin End Total Normality (N) ofEDTA pH Fnctor or Multiplier l reference number standard number -** r-- ml - ml fr;'\ = 0.2/E (acccplnblc r:mgc = 0.0180 - 0.0220) ... (Nx 50000)/ 50 ml sample =Nx 1000 u o~n-l lb n orntorv contraI stnml arr.: Reference standard True value I Sample Hardness (MY)  % RS= MY /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (ncccptnblc rnngc l T\JSS IL\-~ (mg CnCOJ/L) 40 (ml) 50 lq.5 ml ml ~.4 ml I.Cf i,o.o 3~ = 90 to 110%) C\S.o 0 fi, D 11p,Iicnte sample prec1s1011: l Sample Sample ID Sample volume Begin End Total Multiplier Hardness (mg CaCOJ/L) %RPO = ((S- 0) /((S+D)/2)} x 100 number llvA- (m l) ml ml ml t..o.o s L'"\ t>Sb'~,. \ 'i:° ~,u 101 4f'l4 ~g .. .... . . '* .1 3 5v ~."\- 11.i 3!-+ ~ Duplicate (B) J_ 11.'~ JS.'2- 3A l D la~>> l!6i.. 05~'1-J\!lntnx sm (e recover,1: J Reference st:mdnrcJ Spike v:ilue Snmple Spike hnrclncss (A) number (SY) volume .Begin End Tolnl Multiplier (mg C:iC03/L) IJ<.c. J 11.te-:} (mg CaCO:VL) 1-\-0 (ml) 5o ml I\.~ ml rt.1 5.3 ml "],.o .o \O l.# -\lO S:imple hardness (B) Measured spike value (MY)  % R = MV I SV x 100 (mg C:iCOJIL) MV=A-B (acceptable r:rngc (m~ CaCOJ/L) = 75 to 125%) t,i 38 l~J..) <i.5.o°to ltt:&{ samp,l e 111eas11reme11ts: Sample volume Begin End Total H:irdncss Sample number Snmple JD (ml) ml ml ml Multiplier (mg CnCOJ/L) J TV=NO Blank n ,.... .. ,,..._ ._, *.1"\9-}lo~ *-* *'-' L L1 D'Sic \. a:> -rvA 6Qi..) , oH>u \ 5D 1':1.1  ?~.'J, g,1~ ~lO v4 I J l\O~b"').:,S  ?- &,:; _J' -Z0 .3  ?..3.1.,, L,1..,, no~os.n 3 '12 \,. 'h.. .<t -'< ~ I .Lo J noso \. c'-1 - \JltSQ.v... \\)\ I ~UV 1 1.1#.'l -:\~. \  ?>.'2.. L..14- \"'\ o~(P.). ~'-= I 2- dO.I 22. q 3$ L.\4 J l\OSOS* l~ ..l 3 ..... ....- ~-~ 1:2.il,, q 3ff .... -  ::Jo I I - I ~~ C$Q/*<-J-Note: If >151111 of titront is used, sample must be dilulcd. Reviewed by: Date reviewed I O.S*OCo"l'1 Page 66of94 SOP C7 - Exhibit C7. I, revision 01*01-13 l ,:.~ r~£ -

.

. I ..%d Bf;f&l tf?/01 )* . . . -  ;- * ,~.,, . ' '--.... 1 *J. Page_~~of J Page _g__ ~ C..-."./ l -*' \ L::...J ------- ..... : Envilonnnnt.al Tc.it Ing SolullonJ. Inc. Har dness (SM 2340 C-1997) l Analyst \.U I RL = I .0 mg CaC03/L Time initiated I:j}(j:o=:o iz:J02 1 Date analyzed l)?.01-* \1- Time completed  :~e;;...-_-_-_-_-_-~~~ Titrnut uormalit aml 11111/ti lier deter111i1111tio11: Titrnnt Normality check Begin End Total Normality (N) ofEDTA pH Factor or Multiplier l r eference number standard number ml ml ml (E) =0.2/E (ncccptnblc r:mgc = 0.0180 - 0.0220) o.owo = (N x 50000)/ 50 ml sample =Nx 1000 l Lnborntor co11trol stmul11rtl: Reference stnndnrd Tr ue value Sample Hnrdness (MY)  % RS= MY /TV x 100 number (TV) volume Begin End Total Multiplier (mg CnCOJIL) (ncccptnblc rnngc l (~ (mg CnC03/L) 40 (ml) 50 ~-S ml ml 2\.<; 2..0 ml zo o 40 = 90 to 110%) HXHI. Dup/"/C(lfe sampI e precmo11: l ~ ,..,.___ Sample ID Snmple vo lume Begin End Total Multiplier Hnrdncss (mg CnCOJIL) %RPO"" {(S - D) /l(S+0)/21) x 100 number (ml) ml ml ml l ~ Duplicate (B) ~ s D .. " ~ Matrix spike recovery: ~ Reference stnndard Spike value Snmple ~ Spike hnrdness (A) ~ number (SY) volu me: Begin Totnl Multiplier (mg CnCO.i/L) (mg C:iCOJIL) (m l) ml ml ~ "'-.. Snmple hnrdness (B) Measured spike value~ %R = MY I SY x I 00 (m1t CnCQ3/L) MY=A - B (ncccplnblc rnngc (mg C~CO..i!Ll ~ = 75 10 125%) ~.. oan-1~ J samp.I e meosuremeuts: Snmple volume Begin End Tot:il H:irdness Snmple number Snmple ID (ml) ml ml ml Multio lier (mg CnCOJ/L) J TV=ND Blank C,.<; C\.s o.o w :o tvo {should be= 0 mg CaCO;/Ll 8J noc; o\. ~ So.~ lO\ f 50 ,.,,-; :J4.v ~.I lo "2. J \.\US.~:~~ z.. \ iY*IP ,n*.£1 ~. ~ li~ no<.:,ei~. \\ \ 3 l ~1-'1 3 1.2. 3.'3 (Q (p J ~ / M 'f'. - --~ - ------ ,_.-- - ---- Note: If >151111 ofti1rn111 is used, sample musi be diluted. Reviewed by: Date reviewed I 6S*Gi'\1 Pa!ffl a{JL~I o cl ,l ~ '1'- SOP C7 - Exhibit C7.l, revision 01-01-13 Sequoyah Nuclear Plant Biomonitoring May 02 - 09, 2017 Appendix 0 Reference Toxicant Test and Control Chart Page 68of94 Pimephales promelas Chronic Reference Toxicant Control Chart Environm enta l Tes.unq so1uuon1, Inc. Source: In-house Culture 1.2 Control Limits(+/- 2 Standard Deviations) 1.0 ----- . _ ...._. __ __ _ ________ .__. ,,,,,,_._ . - --- ------ . 0 .8 * * * * * * * * * * * * * * * * * * ., , ., , ----* -u

r:::

0.6 ,_..-------------- --------- ~ 0 .4 -d b.O in 1.2 > ta Warning Limits "'C I 1.0 **..*....... ... ..... .... .... .... .... ..... ...... .. ... ....... . . ......... .......... ~ " ** .--. .. - **- **-**-* *- **-...**- **- **- **--* *- **-** .... .__ .. 0.8 * * * * * * * * * * * * * * * * * * * *- **- * *- * * - * * - ** -- 0.6 .*- **--*** __. .. ~ * * -* *-* *-**-* *-**-* *--- **-**-** - ... .... .... ... ...... .. .. .... ... ..... ..... ..... ...... .. ... .... ... , . .. . . .. , " , .. .... ...... .. ' .. . .. . ... .. ... . . . ...... . . ~ 0.4 Test date e 7-day 1Cz5 = 25% inhibition concentration . An estimation of the potassium chloride concentration which would cause a 25% reduction in Pimephales growth (calculated using ToxCalc) . Central Tendency (mean logarithmi c IC25 converted to anti-logarithmic va lues) - - - Control Limits (mean logarithmic IC25 +/- 2 standard devia tions converted to anti-logarithmic values) - *. -

  • Laboratory Warning Limits (mean logarithmic IC25 +/- 2 coefficent of varia tions converted to anti-logarithmic va lues)
  • . . .. - . . . USEPA Warning Limits (mean logarithmic IC 25 +/- SA_75 con verted to anti-logarithmic values, sA.75 = 75 1h percentile of CVs reported nationally by USE PA)

Page 69of94 Pimephales promelas ~ Chronic Reference Toxicant Control Chart lQ CD (nlffto~nMIT.,tt~ Soknlotn. ll'K.. Source: In-house Culture ........ c ~ Log 10 Conversion Anti-logarithmic Values (g/L KCI) co T4',t numbe r Test dat e 7-day IC 25 Laboratory Ca lculated CV 75th Percentile CV ToxCa l Determination 7-day IC25 CT s CT Cont rol Limits Warning Limits Warning Limits (g/L KCI) CT - ZS CT + ZS CT - ZCV CT + ZCV CT - SA.75 CT+ SA.7S 1 04-04-16 0.7240 -0.1402 -0.1647 0.0544 0.6844 0.5328 0.8791 0.4629 0.9689 0.4243 0.9506 2 04-05-16 0.7762 -0.1100 -0.1569 0.0538 0.6968 0.5440 0.8925 0.4775 0.9777 0.4320 0.9679 3 05-10-16 0.7510 -0.1 244 -0.1528 0.0511 0.7034 0.5559 0.8899 0.4937 0.9685 0.4361 0.9770 4 05-17-16 0.6409 -0.1932 *0.15 73 0.0496 0.6961 0.5539 0.8749 0.4918 0.9530 0.4316 0.9669 5 06-07-16 0.6998 -0.1550 -0.1571 0.0468 0.6965 0.5614 0.8640 0.5026 0.9370 0.4318 0.9674 6 06-14-16 0.7412 0.1301 -0.1546 0.0451 0.7004 0.5690 0.8G23 0.5127 0.9315 0.4343 0.9729 7 07-12-16 0.7965 *0.0988 -0.1 5*J O 0.0460 0.7080 0.5729 0.8749 0.5172 0.9437 0.4389 0.9834 8 07-19-16 0.6348 -0.1974 -0.1536 0 .0459 0.7021 0.5682 0.8674 0.5114 0.9376 0.4353 0.9752 9 08-02-16 0.7850 -0.1051 -0.1502 0 .0460 0.7077 0.5726 0.8746 0.5168 0.9436 0.4388 0.9830 10 08-09-16 0.6703 -0.1737 -0.1517 0.0447 0.7051 0.5739 0.8664 0.5190 0.9339 0.4372 0.9794 11 09-12-16 0.7241 -0.1402 -0 .1510 0.0433 0.7063 0.5786 0.8622 0.5255 0.9270 0.4379 0.9810 12 09-12-16 0.7351 -0.1336 -0.1500 0 .0421 0.7080 0.5831 0.8596 0.5315 0.9222 0.4389 0.9834 13 10-04-16 0.7791 -0.1084 -0.1477 0 .0420 0.7117 0.5864 0.8638 0.5357 0.9254 0.4413 0.9886 14 11-01-16 0.7808 -0.1074 -0.1456 0.0419 0.7152 0.5897 0.8674 0.5398 0.9280 0.4434 0.9934 15 12-06-16 0.8303 *0.0808 -0.1423 0 .0433 0.7206 0.5904 0.8795 0.5399 0.9411 0.4468 1.0009 16 01-10-17 0.7066 -0.1508 -0.1433 0 .0432 0.7189 0.5892 0.8773 0.5384 0.9392 0.4457 0.9921 17 02-07-17 0.7052 -0.1517 -0.142.1 0.0426 0.72 09 0.5924 0.8772 0.5426 0.9378 0.4469 0.9948 18 03-07-17 0.7620 -0.1 181 -0.1362 0.0365 0.7308 0.6178 0.8646 0.5761 0.9139 0.4531 1.0086 19 04-04-17 0.7043 -0.1523 -0.1392 0.0351 0.7258 0.6174 0.8531 0.5765 0.9012 0.4500 1.0015 20 OS-02-17 0.6740 -0.1714 -0.1371 0.0316 0.7292 0.6305 0.8434 0.5939 0.8858 0.4521 1.0063 Note: 7-day ICis = 25% inhibition concent rallon. An estimation of the potassium chloride concentration that would cause a 25% reduction in Plmepholes growth (calcula ted using ToxCalc). CT = Central tendency of the ICn values. S = Standard de~iation of the IC1s va lues. Control Limits= Mean logarithmic IC15 +/- 2 standard devlatlons convert ed to ant -logarithmic values. Warning Limits= Mean logarll hmlc ICu +/- 2CV ors. 75 converteCI to anti*logarlthmic values, S,.n = Standard deviation correspoMing to the l':i' percentile ol lVs reported nationally bv USEPA is.,. =0.38). CV = Coe(flclenl of variation. Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture 1.25 .s= ...... - Cl.I ro ....:>ro 1.00 ~ 0 .... 0.75 --- --- --- -- -- --- --- --- -- * \.!) ro ---. --- 0 ... :-c:e a.so ...... *--- -- .....

  • c: .........

0 u tlO .-., --- --- --- --- --- --- --- --- -E 0.25 USEPA Minimum Acceptance Criteria (> 0.25 mg per surviving larvae) 0 0 USEPA Maxi um Coefficient of Variation Guidance Criteria(< 20%) c: ..c ... .52 3 - 20

  • ~"'

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USEPA Upper PMSD Bound Acceptance Criteria(< 30%) 30 USE PA - ~ 0 20 Lower PMSD Bo und ___ --- __.....- * - ----------- ..- *

  • 10

* * * * * * * * * ~~-------~------- ---------- - ------------ 0 o.:...b 'f/>b 0,'\.b 1 .'\.b " 1 .'\.b o.-">b ,_.">1:> ,"J-">b r;,1.sb c"J*\.b ">i.:'\.b ">,,:...b r;,bsb cv">b cb'">b 1Y'°1 01 :...1 01 :...1 & ..,.1 ,_..">1 0 0 0o.!u 0o.* 0<:/'~ r;:,s-"> r;:,b"' cb*'\J 01 -">- r;:,1 *,. r;:,'b' r;:,'b* r;:,"J' c"J* '\.c- ..,_v ">1.: r;:,V"> r;:,1.* r;:,,, c°" 0c,* Test date

  • Control Growth, Coefficient of Variation {CV) or Percent Minimum Significant Difference (PMSD)

PMSD is the percent mi nimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is nor a minimum acceptance criteria. Central Tendency (mean Control Growth. CV or PMSD) 95% Confidence Interval (mean Control Growth, CV or PMSD +/- 2 Stan dard Deviations) Pimepha/es promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ~ ToxCa l Determination Control Growth Control Growth CV Test PM50 Control Control Growth Test (mg/uu1l1l larvHI !l<I (%) Test number Test date Survival Mean CV MSD PMSD CT 95% Confidence Interval CT 95% Confidence Interval CT 95% Confidence Interval (%) (mll/lnlllal l*rvae) (%) (%) CT

  • 2S CT+ 25 CT *25 CT+2S CT
  • 25 CT+25 1 04-04-16 100 0.461 5.9 0.0395 8.6 0.6125 0. 415 0.810 5.3 0.0 10.6 10.7 5.8 15.7

 ?. 04-05 *16 100 0.647 12.3 0.1138 17.6 0.617 0.435 0.799 6.3 *0. 8 13.S 11.7 4.8 18.6 3 05-10-16 100 0.541 7.0 0.0614 11.3 0.608 0.431 0.785 6.4 *0.2 13.1 11 .7 5.3 18.0 4 05-17-16 100 0.507 1.8 0.0438 8.6 0.597 0.418 0.775 5.9 -1.0 12.9 11.3 5 .0 17.6 5 06-07-16 100 0.421 5.0 0.0592 14.1 0.579 0.377 0.781 5.8 -0.7 12.4 11.6 5.4 17.8 6 06-14-16 100 0.455 3.2 0.0334 7.3 0.568 0.362 0.773 5.6 -0.B 12.0 11 .2 4.8 17.6 7 07-12-16 100 0.578 8.8 0.0763 13.2 0.569 0.373 0.765 5.9 -0.5 12.3 11.4 5.2 17.6 8 07-19-16 100 0.774 10.1 0.1053 13.6 0.584 0.365 0.804 6.2 -0.4 12.7 11 .5 5.5 17.6 9 08-02-16 100 0.570 3.4 0.0474 8.3 0.583 0372 0.794 6.0 -0.S 12.S 11.3 5.2 17.4 10 08-09-16 100 0.699 3.5 0.0827 11.8 0.591 0.379 0.803 5.8 -0.6 12.2 11.4 5.5 17.2 11 09-12-16 100 0.604 S.1 0.0532 8.8 0.592 0387 0.797 5.8 -0.4 ll.9 11.2 5.4 17.0 12 09-12-16 100 0.637 4.8 0.0520 8.2 0.595 0395 0.794 5.7 -0.3 11 .7 11 .0 5.2 16.8 13 10-04-16 100 0.588 3.2 0.0506 8.6 0. 594 0.401 0.788 5.6 *0.4 11 .5 10.9 5.1 16.6 14 11-01-16 100 0.671 7.7 0.0627 9.3 0.598 0.A07 0.790 5.7 -0.2 11.S 10.8 5.2 16.4 lS 12-06-16 100 0.617 S.6 0.1167 18.9 0.599 0.413 0.786 5.7 o.o 11.4 11.2 4.6 17.8 16 01-10* 17 100 0.587 7.3 0.0629 10.7 0.600 0.414 0.786 5 .7 0.0 11.3 11.2 4.6 17.7 17 02*07-17 100 0.579 11.5 0.0751 13.0 0.591 0.420 0.763 6.2 0.3 12.0 11 .4 4.8 17.9 18 03-07-17 100 0.638 6.2 0.0662 10.4 0.590 0.421 0.759 6.2 0.4 12.0 11 .2 4.7 17.7 19 20 04 17 05-02-17 100 100 0.620 0.729 5.5 5.6 0.0919 0.0792 14.8 10.9 0.591 0.596 0.<122 0.761 6.3 0.6 11 .q ,, ' 11.4 4 7 17 9 0.416 0.776 6.2 0.6 11.8 5.0 17.8 Nore ; Con trol Survlvaf = USEPA min mum te.n u'eotabUtty c11teria l. 80".4 s1.1rv1val. "'ontrol Mean Growth:;:;: USEPA mloimutfl rest cc.ee1na~fii 1 y cntena ~ 0.25 m1/surv1v ng lilNU*. OJ ~ Cof!ffte~m or \'irtiJ:dor for contro11rowth. 1 USEPA miJ:dmum (\/ g ukf~lltt c,1t~ria (90 "' percenlite, < 10% MSD

  • Minimum >i&nlftcont d fleronce .

PMSO Pcrctnt minimum Stgn1f1nttt difference. PMSO ,,. .a measUt of ~e n prec ision. The PMSO ts 1'1e m1n11nurn percent difte,tmce betw,en the control and lrHrmec,t that can be: declarfd stau~Outly ngniflcitrn In 1 whote elftucmt to'l! IClty tMt. Lower PMSD bound determined by USEPA (10'" percon11le) > 12%. 'The bw~' PMSO bound rep resents .i pr.cticaf ttmir to the senmlVltv of tM te st rMttiod ,;,nd IJ not 1 min.mum 1<<epu1nce a 1tena . Upper PMSD boond *a:eptonco criteria dotermlned bv USEPA (90" percen1llel < 30%. ct'=: CPntr."I ria ntf*f\(\I ot th! l'OWlh, CV o r PMSOv~lu" . s~ St andard devi1tl0rlot the growth, C\/ o' PMSOv lues. .., ~ 11'!--  ;:-""' 0 r-:-::-:i r--:-i ,,,.-...-.

  • I 'I -'-' - 1 - ** I **-- Page I of6 I - -.

... tnYironrMnulTf'stlng Sc.lo1'°"s. l1K. Potassium Chloride Chronic Reference Toxicant Test (EPA-822-R-02-013 Method 1000.0) Species: Pimeplzales prome/as PpKCICR Test Number: 25 Dilution re arntion infor111atio11: Comments: KCI Stock lNSS number: lNSS \ S&i.o Stock preparation: 50 g KCl/L: Dissolve 50 o KCI in 1-L Mi ll i- water. Di lution prep (mg/L) 300 450 600 750 900 1050 Stock volume (mL) 9 13 .5 18 22.5 27 31.5 Diluent volume (mL) 1491 1486.5 1482 1477.5 1473 1468.5 Totnl volume (mL) 1500 1500 1:500 1500 1500 1500 Test oruan;sm informatio11: Test iuformntion: Onranism source: In-house culture Randomizino tem late: Age: < 24-hours old Incubator number and shelf location: Spawn date: Artemia CHM number: CHM914 Hatch dates and times: <:>'>*Ci\*\"'\ \""\OQ ~ Drying information for weight o<:..-o-i.-n ~t.ooS determi11atio11: Transfer vessel information: pH= \.21.. S.U. Date I Time in oven: Temperature = "l..~.C\ °C Initial oven tern erature: lDa*c_ Average transfer volume: < 0.25 mL Date I Time out of oven: O'MO*"('\ 0 Final oven tern erature: Total drying time: Daily feeding aud renewal information: D:i y D:itc Morning feedin g Afternoon feedi ng Test in itiation, renewa l, l\fl-ISW or termin:ition batch usccJ Time Ana lyst Time Analyst Time An:ilyst 0 05-02-17 ()o\0 X{ ... \'1.~o u o~ "1..0 ~ o'-l*u.*11 A I 05-03-17 c~ ,.{ \~c.c  !<" 09~'-\ ' 0'\* 11o. ,, ~ ~"' \1 2 ~1 \,I. 05 17 D\oO C,, n . . o~ u o<;;.~c=\ o+t.b*f'\ & 3 05-05-17 Ob\C ~l \"l.l b l( 0&'°'40 \\ o'-l

  • i.~ . ' , "'

4 05-06-17 C"l\ c; " \~\<,. ~l ol\\5 i\ O~*Cfl..*\1 5 05-07-17 0"#\0 't\ \ "!l'\C H O'\t..\O At OS*Ol.*'1 6 05-0R-17 Olo \<':, v }t \'1.\.<.> i\\ - o'\01- Ai CS*<n.. ,, ~\ 7 05-09-17 o i "to - Control illformalion: Acceptnnce criteria 0 . $ 20% Average weight per surviving larvae: 2: 0.25 mg/larvae Page 73 of94 SOP AT21 - Exh ibit AT2 I. I, revision l 1*01 -14 ~ r? ~ (;::i Page 2 of6

I --

,. I. *. -,...__ '"i --- I -

*-

_ . _.../ EnvlronrTtt.nt~I Tc~Ung SoI11Uans.lr.c.. i ) Species: Pimeplzales prom elas PpKCICR Test Number: 25 S urvival an d Growth Data Day Con tro l 300 mg KC l/L 450 mg KCl/L A B c D E F G H I J K L 0 10 10 10 10 10 10 10 10 10 10 10 10 l /D <o IO to 10 IO 10 Jo 10 10 IQ 1a 2 t~ lQ to (O {Q IO IQ \C) LC) lo IO 10 3 (() I\) (Q lb I() IC) 10 10 I\) I {J IC 10 4 10 10 /0 JO 'O IQ /0 IO ID I() IQ IQ 5 I 'O It) ID IQ LD { () IQ ( () co IQ LO 10 6 JU m f{) (0 lO 10 I () 10 IQ l() lO 10 7 ., 0 ft) I() ID 10 ro to lb 10 ID ( () 10 A = Pan weight (mg) Tray color code:: U:g h I- BL" .e. 15.IS 15.~ j \,,}.S lb.'18 15 .'°40 l3 .~2 1-:iOO IS.e=f Annlyst: 'i-- IS.3s llo.18 \4 .~z. ls. °37) Date: OY* Z:Z-1~ 13 = ran + Larvnc weight (mg) Ana lyst: lA...J ~~.le J.~.'-ll ?13.88 ~,,a, citl.'5l> ltt.~4 ~ *.oe ~.16 ia1 .q~ 22.cH io.'¥1 ~o.1~ Date: os.11 .n-C = Larvae wc.ight (mg)= B - A Ha nd cnlculntcd. h.Co~ , ,olo 1 . 1..~ ". &?>> <..to S,t\"L '-* () 2 , .'?..'\ Co.SC\ s. C. c. . a1 SA"\ Ana lyst: ~ Weight per inil inl number of la r vae (mg) =C f In itial number of lnrvne It') ,...~ ,...~ fl.,,., oi ~ ~ 6' }- Hnnd cnlcu l:i tcd. £ ..,0 '? (J..'V . \!) " "' "'6'.. . t.; \0 ~ t '1) A1rnlysl: (). ~* () . '()" o*"' o* 'V C) ' 'V' 'I;) o* t$ ,_ ... . ..- r. ..-- Average weight per inilial Pc recur number of J:ir"ae (mg) red uction from con lrol 0 .11..'\ l~-t~~' *. ef -Ll~.* .. *'> 0f o . b~S \ '2. .'\ 1. o. ~a.Ci n . &<. ("/o) .:-.: *~* *-. . Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk =s ick, sm = unusually small, lg = un usually large, d&r = decanted and returned, w = wounded . Comments: Page 74of94 SOPAT21 - Exhibit AT21.J , revision I J-01-14 Page 3 of6 EIWfron men1aJ Teilin9 SGlutJOns., lnc. Species: Phnep!tales promelas PpKCICR Test Number: 25 Survival and Growth - - Data Day 600 mg KCl/ L 750 m17 KCl/L 900 mp KCl/L M N 0 p Q

  • R s T u v w x 0

10 !O 10 IO 10 JO JO 10 10 10 10 10 J 10 ID IQ I{) 10 I l) ( () co IO "11{ 10 ' \ t .( 2 °" '*~ 10 10 10 tO 10 ro ro '() 10 ~ 3 10 ID ID l () ID t() J 0 I() 10 ~ '\ 4 (0 LO 'D io l \.) \~ ~ l#. "l-'. & ,. '\ °'  !'*~ .f'"' 5 '6u.. 10 ~~ IQ ~ "&~ ~'"' K f .,'"- ).\ ~ 6 g 10 \ \ -,'A l ~ , "'° ~ ~ " ~ 10 lo 7 - - ~ \ () ~ H) \ ., , ..( t - - \ \. \.. A = Pan weight (mg) '1.u "" Tr:ty color code: :  :=.h:qM e. l'i6Lt 1c;,qz. IS.e;.t 15.£:\~ is. ~o IS.le lto.6~ Analyst: ~ l"i.llv IS.l.I.{ ll0.12, I "~:t IS.03 Date: o~. a.";}. ~r"l B .. Pan + Larvae weight (mg) ~ Do.41 a~ .3l l.t.ll. ~o.oe 2o.£fz. 20.2e a..o.~ \'t1S IS.&=\ 2o.11 Analyst: Dale: o:;_ u.1-:r ~1 .~8 l'f ' °' C = L:irvac weig ht (mg)= B - /\ Hand calculated. S.ii 1...4'; i.. c'-\ ".iii. 'i.8'1 q.to t.(.U s.t~ ~.10 4 .~~ ~. \.\ :>.1.-1 Analyst: 'W eight per initial numllcr of larva e (mg) J = CI Initial number ofl:1rvnc ,.. N ~,,,, ~ <,,~ t\/ Hand cnlculnted. ~ $' 0 ~ ~ v,O ~"' "'o .).. ~ ~ .;j .  ::;;. ~ v .)..: ~ '? Analyst: " o* o* t)' o* ~r o* o* A\'erngc weight per initial Percent 0 ~* tr "' c;)' number of larva e (mg) reductio11 o.1o'2.& r~ . ~ 1. 0 . 1.\1~ ~"\.~1. o. ~'15 ~s . C\ 1. from control (% ) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = mi ssing, sk = sick, sm = un usually small , lg = unusually large, d&r = decanted and returned, w = wounded. Comme11ts: SOP AT2 l - Exhibit AT2 l . 1, revision l 1-0 1-14 Page 75 of94 0 0 ,- I '-

  • L-::J r;::J.

.

I . -. Page4 of6 Species: Pimephales promelas PpKCICR Test Number: 25 S urvival mu/ Growth Dain Day 1050 mg KCl/L y z AA BB 0 10 JO JO 10 l ,~ ,~ t. "\...\ lo'4~ 2 l.. .... !Q'" (g to 3 '-\.,,.,. s ,.... \, \D 4 ,;,. v.... w.. "'.I. "'L ~ ~ L- ,.... 5 l '\A t '" "'Z... \*~ '" '" c"' 6 \ () \ 7 \ t:> \ 0 A = Pnn weigh t (m, .ci. ,..,...,.. 'i.. l~l+ &sve., ~*"' Tray color code:: Ana lyst: ~ l110'i ~ 11o.l8 ~ Daic: CH . ~~-n B = Pan+ Lan*ae 11*eigb1 (mg) 11.ot t~'lll ~~ n:1ly t: Dntc: """"

05. }l .j'.}:: 7 6t C "' Larvae weigh I (mg)= n- A o."10 Hnn tl cn lc ula tcd. 0 ;1'&) ~ i."' ~

,......... Analys t: ~\ I>.. Weighl per in itial nu mber ofl:tr\'ne (mg) "' CI Initia l numbe r of lnr\'nc Hnnd enlculatcd. ~,..,, 0 \..' <:> 0 Analyst: ~ ()' ()' Avcrnge weight per inili al l'crec nt number oflnrvnc (mg) rcducrion o . o~t:\ ~'{.11. from con trol (%) Comment codes: c =clear, d = dead , fg =fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg = unusually large d&r = decanted and returned, w ,,.. wounded. Comme11ts: Page 76 of94 SOP ATI 1 - Ex11ibit AT21. I, revision 11 14 Plmepho/es promelos Chronic Refere nce ToKica nt Test EPA-821-R-02-013, Method 1000.0 Quality Control Veri' ication of Data Entry, Calcula tions, nd Statistical Analyses TOll number: 2S ~-----------~ T*tt dates: _ _ _ _ _ ___M_*v_o_2_-0_ 9_._20_1_1 ,_.,,.,.11o"'"'"l""' "*.Mtel* 111111* !......m\*tDI '*"'** ...... rl".tm.mb.,ot ,...,_ ..tttit(,.-icJ I

  • Patt* l*fn*

wefcht(md ... a...,..,,,. ...,.,, (rnd

  • Waltftt/S**vt,...* 1 M. . .. wt l ~/ Swvtwf*&

nwnbu of hrv** Imel 11umtt.1 ol tr"'n lm1l ~ ..... .................... C'at md* "lofvwlaciori _. - **1ooo*....1tKI W*l &h1/~1lljn~*** l~(lftfl M.. 1't1H¥1"~(l') ~~aJ'i w.fit\l/ ~ftut Cocffhflf'll Of Wot tUOI\ ttul'l\beroJ l11 rvH tml) 11 r.u'" r*dl.kUon " " ' (Gft! Utf '"'* A 10 10 IS. IS 22.78 7.63 0 76? 0.763 8 10 10 lS.3S 22.41 7.06 0.706 0 .706 ConUol 0 .129 5.6 100.0 0.729 5.6 Not applica ble c 10 10  ! 6.2S 23 88 7.63 0,763 0 .763 D 10 10 ls.98 22.81 6.83 0.683 0 ,683 E 10 10 15.40 2150 6J.Q 0. 6~C!,__ 0.6 10 F JO 10 13.42 19.34 S.9' 0 592 0.592 JOO O.E35 10.0 100.0 0.635 10.0 12.9 G 10 10 15.00 21 08 6 08 o.608 0.608 H 10 10 15.1!9 23 .l& 7.Z9 0.729 0.729 I 10 10 15.35 H .94 6.59 0.659 0.659 I 10 10 1618 12.04 S.86 0586 0.5!6 450 O.!i99 80 100.0 O.S99 1 .0 17.8 K JO I 10 14~7:? 2079 6.07 0.607 0 .607 l 10 10 1530 20.74 5.44 0 .544 0.54* M 10 8 14.54 2041 5.87 0.734 0.587 N 10 10 15.92 22.37 6.45 0.645 000 600 081 S.5 92.5 0.628 6.4 13.8 0 10 9 151"' ll.BS 6.04 0.671 0.60-I p 10 10 14.96 21.n 6.76 0.676 0,676 0 10 7 l S.2* 20.08 4.84 0.691 0 .48.> R 10 7 16.12 20.92 4.80 0.686 o.~ 750 s 10 7 14.97 19.19 4.22 0.603 0659 6.1 0412 71.S o.*18 8.9 34.4 T 10 g 15.()3 10.28 5.Z5 0.656 O.S15 u 10 7 15.95 20.65 4,70 0.671 O.A70 v 10 6 IMO 19.7S ,,n 0.725 14.l 0.4J5 62..S 0 .395 18.1 9UU 0.630 4S.9 w 10 6 15.78 18 89 3. 11 0 .518 0.311 x 10 6 16.57 20.19 3.6? 0.603 0362 y 10 I 16.** 17.07 o.n 0.730 0.073 10$0 z 10 0 0 .00 0.00 0.00 0.000 0.:10 7.l 0000 s.o 0.039 US.8 94 .7 AA 10 I 16 IS 1699 0.81 0.810 0081 88 10 0 0.00 0.00 0.00 0000 0.000 >unnctrs MSO 'lillue i 0 .0 792 M$D : MtlWl'tum SlcnFic.lnt Olffe,..,,CC! 'NISD: 1 0.9 PMSO : P-~rcont Minimum S*snlfte*nt Difference PMSO ti a rnHuirt of tut i:iredsico Tht PM.SO b ~ h e minimum otr(ent cllfferenct: t>etwnn the c°"trot 1nd trutrnenl that ati be decf.-rcd il1ttistieallv s ift n ifk~t1I In a wttole effluent tow1dty test Lower PMSO bound cleterMined brt USEPA ( 101h PfHC'tntlle) :s 1l t-4 Up~r PMSO bound det*rmlncd by USEPA (90th perc:enllle) .;;: 50'4. l ower and pp.er PMSO boufld\ wen detcrminud from the 10th 1nd 90th pernntile, P.t t1 ety, of PMSO d:.lt;i from ( PA's WET ll'tterbbOr.Horv Varl~bllilV' Slud°' ~USEPA, 2001~ USEP". 2001b~ The fowe1 PMSJ botmd ropunef'IU

  • PrKllul llm{l lo th~ i ansitwlh of the test method iltid is not a minimum ~:epunc:e: critcrii tJS!PA 20011, 2001D. Flnal Reoon: 1tut1rl1tDOnfOl"J vat1abU11y !:t.hJov of U'o\ Short-te.rm Chtonie at\d Acute Whole Elt l\Jent To*icltyTest Me thoch, V~umos 1 tl'ld l*Appctndl'(. EPA*ll:J..S..01..()04 :and EPA.-321-1 *0 1..00S. us Envlfor\mental Prote<tion Agencv. Cln i:lnn~t*, OH .

Sta tistica l Analyses Larval Fish Growlh and Survival Test-7 OaySu.rvlva l St.art oa e- 51212011 Test ID: PpKClCR S;j111µ1 ~ ID. REt>- RefTOAi1,;Cill\ End Date: 51912017 Lab ID* ETS-Envir. Testing Sol. Sample T'fpe: KCL-Po tassium c:hlof!de Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-m IL 2 J 4 D*ContrOI 1.0000 1.0000 1.0000 1 0000 300 1.0000 1 0000 1.0000 1.0000 450 1.0000 1 0000 1.0000 1.0000 eoo 0 8000 1.0000 0 .9000 1 0000 750 0.7000 07000 0.7000 0.8000 '900 0,7000 0.6000 0.6000 0.6000 1050 0.1000 0 .0000 0.1000 0.0000 Transform : Arcsln S9uarc Root Rank 1-Talled Number Total Conc-m9/L Mean N -M11an Mean Min Max CV% N S 11m Crltlr.:ll Ras p N umber D-Control 1.0000 1 0000 1.4120 1.4120 1.4120 0.000 4 0 40 300 1.0000 1.0000 1.4120 1.4120 1.4120 0000 4

  • a.oo 10 00 0 40 450 1.0000 1.0000 1A120 1.4120 1.4120 0.000 4 " 8.oo 10 00 0 40 600 0.9250 0.9250 1 2951 1 1071 1.4120 11 347 4 '4.00 10.00 3 40 750 0.7250 0.7250 1.0202 0.9912 1.1071 5.685 4 ~ 0 . 00 10 00 11 40 "900 06250 0 6250 0 9123 0.8861 0.9912 5.759 4 10.00 10 00 15 40 "1050 0.0500 0.0500 0.2403 0. 1588 0 ,3218 39.161 4
  • o.oo 10.00 38 40 Auxllia!l'. Tests Statistic Critica l Skew Kurt Shapiro-Will<'s Tasl indicates non-normal distribution (p <= 0.01) 0 .87827 0 896 -0.3Be8 1 96258 Eguaht~ o* vanance cannot be connrrned H~Eot hesis Test (1-tan, 0.05! NOEC LOEC c.nv TU Steel's Many-One Rank Test 600 750 670 .82 Treatments vs 0-Control Trlmmed Spearma n -Karber Trim Level EC50 95"/. CL 0.0%

5.0 % 10.0% 876 51 S34 62 920.49 10 ..-~~~~~~~~~~~-, 20.0o/o 897.43 84837 949.33 09 Aul°'S.0% 865.76 826.27 907.13 08 07

lc 0.6

. &. 0 5 ~ Od 03 02 0 I 10 100 1000 10000 Dose mglL Dose-Response Plot 09 08 . > 07 ~ 0.6 ,.." "'0.5 Cl 04 r-03 0.2 0.1 0 g ...g c 0 ll 0 Page 78of94 Statistical Analyses La rv:JI Fish Growth an d Survi val Tc st -7 Day Growth Stan Dale : 5/212017 Te~ I ID* Ppl(CtCR S;omplA tn

  • RFl'°- RAr Tn~ic:;,nt End Oa1;i* 5/9/2017 Lab I~ ETS-Envir. Testing Sot Sample Typ . KCL -Potasslum chloride Sample Date Pro1Dco1 FWCHR -EPA-821-R-02-013 Test Spec1 s PP-Pimephales promelas Comments:

Co n c-m IL 2 3 4 0-Control 0.7530 0 .7060 07630 0. 6830 300 0.6100 0.5920 0.6090 0.7290 450 0.6590 0 .5860 0.6070 0. 54'50 600 0.5870 0 6450 0 6040 0.6760 750 0.4840 0-4800 04220 0. 5250 900 0.4700 0.4350 0.3110 0.3620 1050 0.0730 0.0000 0 0810 0 0000 Trans form : Untransformed 1-Tafled Is otonic: Conc* m !l'L Mean N-Mean Moa n tJlin Max CV% N t-Stat Cri tical MSD Mea n N-lllean 0 -Control 0.7288 1.0000 0.7288 0.6830 0 7630 5 578 4 0.7288 l .0000 *300 0.6348 0 .8710 0.6348 0.5920 0.7290 9.980 4 2 .718 2.290 0,0792 0.634 8 0.8710 *450 0.5990 0 8220 0 5990 0.5440 0.6590 7 .982 4 3.752 2.290 0.0792 0.6135 0.8419 *soc 0.6280 0.8617 0.6280 0.5870 0 6760 6.403 4 2 913 2.290 0.0792 0 .6135 08419 750 0.4778 0.655 0.4778 0.4220 0.5250 6.868 4 0 4778 06556 900 0.3945 0.5413 0.3945 0.3110 0.4700 18.143 4 0.3945 0 5413 1050 0.0385 0 .0528 0.0385 0.0000 0.0810 1 15.781 4 0.0385 0 0528 A uxillal"t'. Tests Statistic Critica l S k.e w Ku rt Shapiro-Wilk's Teslindicates normal distribution (p > 0.01) 0.92374 0.844 0.70843 -0.4102 Bartlett's Test indicate$ egual variances(!!= 0.66) 0.76291 11 .3449 H~ poth esis Test (1*Uil , 0.05) NOEC LOE C ChV TU MS Du MSD p MSB MSE F-Prob df Ounnett's Test <300 00 0.07919 0 .10867 0.01266 0.00239 0 0148 3 12 Treatmen ts vs D-Control Lin crar lntcrpolotl on (ZOO Rc::;amplc:; ) Point mgIL SD 95% CL(Ex p ) Skew 1co5* 116 29 61 .66 54 .14 436.34 1 8870 1c10* 232.58 85.50 108.28 6D2.01 1.6404 IC15 408.09 138.74 126.89 754 .60 0 3100 IC20 633.70 58.72 316.33 686.56 -3.8530 0 .9 IC25 673.96 19.94 613.55 747.38 0.2701 IC40 822.97 41 .45 7 15 37 958.28 0 3965 0 .8 ICSO 912.69 18.66 829 43 945.96

  • 1 0693 07
  • indicates IC estimate le$$ than the lowest conc.en tration

=: 0 .6 c: .g_ ~ 04 0.5 OJ 0.2 01 500 1000 1500 Do se mg/L Dose-Res ponse Plot 0.9 0.8 07 1 -!all, 0.05 level i 0.6 o f s gnlllcance e o.5 Cl

04 0

... 0.3 01 0 I 0 ec 0 0 6 Page 79 of94 ~l *.-t-i ~ .P ., Page 5 of6 .* 1 Species: Pim eplzales promelas PpKCICR Test Number: 25 Daily C!temist1y: Day (Analvsl identified for cnch day, pcrfom1ed pH , D.O. and conductivity measurements onlv.) 0 I 2 Ana lyst t- .,;... ~ Co nccn tmtion Pa r ameter 1l1

.;- '. ~-

-* IL-Q * . .. -. "'"" .- .- - *~  :-:~ ,_ - *" *.. *- - _.__ ~

l. - ~-*~"

pH(S .U.) g,2.\ ".}Pt~ e>.10 "t.':\Z. e.oe -+:~3 DO (mg/L) 't.!1 .'.J.. .to +..:r /l. Y *+.~ '~.2.. Conducfi\'ity . ..; (11111hos/cm) 32..\ . "* -32.6 ..* '3 t<i' I* ' -  ; .. - _> -* ,,,*... - r..,: *;;: . ~-~ ._~I,.. C O NTRO L "Alkalinity ' - (m~ CaCOJIL) " H:irdnc s ~\ , .. ~-:~ <v - '.'[ ,,. '4~ -- -- -* . .. . ~  ;': . . *: .. - .,. (mg CaC01/L) 8~ ~'?.. ~ i~ . ,,_. - *Temperature (0 C) ,_'\."'\ "\S. '1. ~., ,.,1..\. \ 1."'\.l.o "'\.&.\ . ~ p H (S.U.) 0.'1* ".l.e>j ~.n"l- 1-\40 So,p '\.-s:1 DO (mg/L) ~-i:\ f).._\O "+.'l ~. \.( 1-..d ".\A JOO mg KC l/L Cnn<l nrtivity l . ~ . "'* , ..' ~ (um hos/cm) ~2 eq0 ( ett \ .. - *Te m pera t ure (0 C) "1.'\.i... 'L..S."\ -u>.,.\ "l.'-\*S "1..o.\.~ \.."\*'\ pH (S.U.} e.\'5 -1.. ~ &O<P ~.\12 0()5 '.} *" ' l 450 mg KCl/L DO (mg/L) '}.'\ 'l .ta "".}.~ '+.3 /l.A ~.'?i. - Conducli"itY i°'\O ' 10"10 \070 *i (µmhos/cm) " Tcmpernture (°C) "l.'\.<o ~ "'l-..o *~ "'\...\ .a '\_'-\ . ~ L."'\.lo *..r_ ...! ...:~ "'\)\ . ,_ pH (S.U.) 0 .\S 1._~.:;- e,.05 ~.t,O, AD~ ~. \I l D O (mg/L) e.o '.}lb 1\-a ~.t, "\!1 -:\..s . 600 mg KCl/L Conductivity (~1mhus/c111) 13'10 . . ' - low  :* l320 .. .. 0 - ' . -***' 'bl.,_ *Te m pera t u r e (°C) (.~lo '1..."'.* (- "\.'\- & L."\ *5 t.'-\.1 p H (S .U.} 'O.\~ 1-.e4 e.o5 ~.1~n e.02 '+.\10 D O (mg/L) eo . 'l-.~ .. 8.o "\;3 .; Q.Q .. ~3 -- ... 750 mg KCl/L Cond11c1ivity (~tmhos/cm) 161o . lSLIO 16&.J "'Tcmpernture (0 C) 1,"1,.. lc. 'Lt;.o "'\.~ . J .,_"' * '- i."\*1 \.U.-S pH (5 .U.) e.\~ 't.02. ~.o'1 1-.l61. e.03 '+.5t1 DO (mg/L) Q.0 ~."l- t3J:J /l. 3 ~.o .}.3 900 mg KCUL 1.,. -. - . Contl11c1i\*ity (~tmhos/cm) 1e3o ... . le20 - . .. le>\O ~ ..._..... . ..:;.:.~* ... .,.. " Temperntu r e (0 C) 1..L\. lo 1..~.o "l...\ . ~ -i'-\.'4 "'\."'\ . ~ ,,~.s p H (S .U.} g.,,?> ').C'~ 0.o~ 'l ti~ e&+ '+.Sf-1050 mg KCl/L D O (mg/L) A.I +.u ~.o 1-. ~ -. 6.o . "'.}. - .. ';"( ~ . . . 1l Conducli\'il)' .. ,  ; (µrnhos/cm} ~1:50 . ~100 ~ - . . ~\20 -. - *Temperature (°C) 1.1.\."1 "\...*-\.'\ '"l.-!-'\ 1..1.\ *.., 1.c.\. ~ 1."\. lo Initia l Final Initial F inal Initial Final ....... ltn

  • Temperatures performed :ll the time oftest initiation, renewal or termination by the analyst identified in the Doily Rcnew~l lnfonnnlion table loc.-ited on Page I.

w iw *nlin ity nnd hardnCEs performed by the ana lyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: t . \ Page 80of94 SOP AT21 - Exhi bit AT21.I , revision 11-01-14 0 o r I_,_] [_* -l ir.Q l Page 6 of6 r* I 1-: '  :-- Species: Pimeplzales promelas PpKCICR Test Number: 25 Day {Analyst identified for each day. performed pH, 0 .0 . and conductivity measurements only.) 3 4 5 6 Ana lyst ~ Pf:..t , ...... (<.J IA- l.l.__ v--- l)V jJ ,)v Concentr ation Parameter ,,,.. ~ .;. . _,..;..; -- - * *~ IP - " 1~ I ~- 7' -- .r. , - - ,.:;;;_*_...,_  ;!'-. r* *. ,_ .* .  ;*~ pH (S.U.) Pu" -:?.Cf~  ?,.oi ').~"'} 0 .\'5 "t.e2 e.~ 'J.w "":l~ .. DO (mg/L) ~- 1 -i. q ~q ':\.:} ':l-lP .. *. .., ":).,A ~. o Conductivity bZ.U - - ~20

-- .:J

-~ -. - (lam hos/cm) 311- - ' . --* 32'-{ __.. . .,. * .~.*~ .  : ~ -- --. *- . .. *- _ -r.';r_ . .. --- _. !~"'

  • Alkalinity CONTROL (mg CaCO,!L)  :~f'- ~

~~ "... " ~ ......  : .~ - *;~ . ~--- - ~ .. - . - ~ -* v-

  • Hardness .~;;:

g(o '  ; (m!!. CaCOJIL) .. - .,;_  ; *Temperature (oC) ""l!\. \o "\...\..-.\ "1.A. <a '""\."\- lo ..~ . f "\...~-"\  !>\. & t.-l.* ~ pH (S .U.) M1- 1.'15 ~ - \! ')..53 0 .\0 ~-1'll ~ ,r:;: ')-,\.( ~ DO (mgfL) ~~  ?-.'1 J *<6 '\.0 .-:\..~ I'}.~ /l..tl 14. \ 300 mg KCl/L Conductivity , .. - >. (;t mhos/cm) <0'4 \ '6'tt4 **~ - 0&1 0I05 *Temperature '"1.'\.\o "\.-{ . '\ c*q \."\... r "\.4 .\a C..'l* 1 '\."\* ~ "\..'-'*' "\.'\-1... pH(S.U.) 611v.1 1.":.11.1 <( . \2- 'l, Q" ~ .c:A 'llA~ 0..\~ 'i-*62-DO (mgfL) ".\~ +.r -:J A ~. o r:.\-.i- 'l.ra "'.\-.~ (t.3 Conductivity ' 450 mg I CllL {µmhos/can) l011) hC.O h'iO \HO - *Temperature ""\.'\, .lo '\.'\ .1 '""L'-\* \ l,.'{.\ "'-"l*1 \...'-\ *L "l!V\ "1.'\*).. c*ci pH (S.U.) 0-05 "J .'1-'l <6. \ '2.- ~-~ 8.dl 'lM (JI ~ . \~ 'l...sY DO (mgfL) ~ 1"\ f. q S ..J (Oft l.l.~ ').ca ".). t\ ~. 2> 600 mg KCl/L Condncli\'ity \320 ' i ' . {11mhos/cm) \ l~O -- a'-( 2.0 - , l3qo - *Tempernture L_-.\. b 1.1\ * ~ '1..'-l-1 '"\..'-\* (.o l.~-1 L.'-\..1.... L.c.\.'\ '1.'\.- S ('C) pH (S.U .) e.os -:J.'1a ':l-.50 e .~ '+-v~ 6.,\~ .I 750 mg KCl/L DO (mg/L) Conducti\*ity lS9J e,o -:)._" - '"fl.\' i .o ~.,o ' t-d l~':lO .,~*5" - - 00 (~2.0 ~ 'l,l./G\ \(1. ..- 3 (µmhos/cm) \~~ ~' ~ .' I " Temperature ("C) pH (S .U.) l.t.\ ':"'\ 0.o~ C.~*"\ 1-'l l '"\."l-1 ~ . I\ ~-~ ~-5!; '"cl.1 e.oe t.."\.S /)..tJef L."\ .'\ 0.,\2.. )..'-\.~ "\.. ..,~ DO (mg/L) 8,l  :} .-:J.. I.DB q _(3 'l-5" 8.o ~. t 900 mg KCl/L Conductivity 4i * " ,. ~ -.. * (µ111hus/c111) ' 't?bQ ..

  • l.-o\D ,._. f0a'}o _. .:., - ""' h3~0 - .* !

*Tcmpernture 'l..' i

  • l ).'\ .'\ '\."\."" "2..' \-1 \...'-\* 1.. ~ ., l-4..~

(°C) t."\. f pH (S.U.) e .o~ T--:µ; ~ .'09 ~ .vo A . o~ 'l..'1-'\ 0 .\2. 11..UP> DO (mgfL) B.I +.-+- j ,'2.- 4 .~ . - - .. ' ~. e;j r.}.u 'b.l l.O.?. 1050 mg KCl/L Conduclivity (1lmhos/cm) Z \lcO 2-\G:lf) ~ Al'-f 0 ., .... ~'YO ' < *Temperature (°Cl '1.~. lo t't*~ £.~-& 1...4* ~ 1."\. "'\ 1.~. '{ 1~ .c) 1.4*~ Initial Fina l Initial Final Initia l Fi nal Initial Final

    • .,,::::~\:' pcraturcs performed al Liie time oftest Initiation , renewal or 1erminmion by the urrnlyst idt:11ti1icd i11 the Daily Renewal Information table located on Page I.

~lka inily and hardness performed by the analyst identified on tile bench sheet specific for each analysis and transcribed to this bench sheet by: _ . Page 81 of 94 SOP AT21 - Exhibit AT2 I. I, revision l l-01-14 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Envlronmcmt411 Tostlng Solution s,, In c. Source: In-house Culture Control Limits (+/- 2 Standard Deviations) 1.08 ------... -- --- -----*_,.,. -.- .----* ---- - * - -......._._. * *

  • 1.06 * * * * * * *

* * * -unJ ~----~--------~------~------*

  • z_, 1.04

......... -"' u 0.0 N > nJ 1.15 o * '" * .. * '" * * * * *

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  • o * ' * * * *
  • o o * .. '" o o o
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  • o o ' * ., '" o ' * * ' o o ' '" * * ' o o o ' * .._ o * *
  • o
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  • o o *
  • t t . . . o t. .. . t I o "O Warning Limits I

" 1.10 1.05 .. .. . - **- **-**-*****-**-* *- **-**- **-** *- ** - **----**-**-* *- ** **- ** -**-* *~* *- ** * -**- * ** -* *-* *- **-**** . ~ 1.00 .. . . . . . . .. . . . ... . . . . . . . ..... , . . . . . . . . . . .... . . . . . . . . . . . . . . . . ...... . . .. . .. .. . ... . .... . . . . . .. - * * * " .. . . . . . . . . . . . . . . . . . . ..... . f . 4 . , . .. Test date

  • 7-day IC25 = 25% inhibition concentration . An estimation of the sodium chl oride concentration which would cause a 25% reduction in Ceriodaphnia reproduction (calculated using ToxCalc).

Central Tendency (mean logarithmic IC25 oonverted to anti-logarithmic values) Control Limits (mean logarithmic IC25 +/- 2 standard deviations converted to anti-logarithmic va lues) - ..- . Laboratory Warning Limits (mean logarithmic IC 25 +/- 2 coefficent of variations converted to anti-logarithmic values)

  • .**. *.. . USEPA Warning Limits (mean logarithmic IC25 +/- 5A.io converted to anti-logarithmic valu es, s A.io = 10th percentile of CVs reported nationally by USE PA)

Page 82of94 Ceriodaphnia dubia CQ ~ (I) Chronic Reference Toxicant Control Chart W co* f: 1wo :mnwnc.at Ttt' ""SI Sol1,1 11Df'l,-, Inc, Source: In-house Culture -.,.,, 0 (0 Log 10 Conversion Anti-logarit hmic Values (g/l NaCl) Test number Test date 7-d ay IC25 Laboratory Calculated CV 10th Percentile CV ToxCal Detem1lna tlon 7-day IC 25 CT s CT Control Limits Warning Limits Wa rning Li m its (g/l N<iCIJ CT

  • 2S CT + 2S CT - 2CV CT + 2CV CT . SA.10 CT + SA.10 1 01-12-16 1.0562 0.0238 0.0283 0.0031 1.0673 1.0520 1.0828 1.0530 1.0816 0.9819 1.1527 2 02-09-16 1.0581 0.0245 0.0281 0.0032 1.0668 1.05 10 1.0829 1.0520 1.0816 0.9815 1.1521 3 02-09-16 1.0624 0.0263 0.0281 0.0032 1.0668 1.0510 1.0829 1.0520 1.0816 0 .9815 1.1522 4 03-08-16 1.0690 0.0290 0.0281 0.0032 1.0668 1.0510 1.0828 1.0520 1.0815 0.9814 1.152 1 5 04-0 5-16 1.0720 0.0302 0.0279 0.0029 1.0GG2 1.0521 1.0806 1.0529 1.0795 0.9809 1.1515 6 04-05-16 1.0627 0.0264 0.0276 0.0028 1.0656 1.0520 1.0794 1.0528 1.0784 0 .9804 1.1509 7 05-10-16 1.0605 0.0255 0.0275 0.0028 1.0654 1.0516 1.0793 1.0524 1.0783 0 .9801 1.1506 8 06-07-16 1.0538 0.0228 0.0270 0.0026 1.0640 1.0512 1.0770 1.0520 1.0760 0.9789 1.1491 9 07-12-16 1.0579 0.0244 0.0268 0.0027 1.0637 1.0507 1.0770 1.0515 1.0760 0.9786 1.1488 10 08-02-16 1.0662 0.0278 0.0268 0.0027 1.0637 1.0507 1.0768 1.0515 1.0759 0 .9786 1.1 487 11 09-13-16 1.0628 0.0265 0.0269 0.0026 1.0640 1.0515 1.0766 1.0523 1.0757 0.9789 1. 1491 12 10-04-16 1.0768 0.0321 0.0273 0.0028 1.0648 1.0511 1.0786 1.0519 1.0776 0.9796 1.1499 13 11-01-16 1.0785 0.0328 0.0274 0.0030 1.0652 1.0504 1.0803 1.0513 1.0792 0.9800 1.1505 14 12-06-16 1.0753 0.0315 0.0274 0.0030 1.0652 1.0505 1.0800 1.051 4 1.0789 0.9800 1.1504 15 01-10-17 1.0723 0.0303 0.0276 0.0031 1.0656 1.0506 1.0808 1.0515 1.0796 0.9803 1.1508 16 02-07-17 1.0641 0.0270 0.0278 0.0029 1.0660 1.0517 1.0804 1.0526 1.0794 0.9807 1.1513 17 03-07-17 1.0641 0.0270 0.0276 0.0029 1.065 7 1.0516 1.0800 1.0524 1.0789 0.9804 l.1509 18 03-07-17 1.0614 0.0259 0.0276 0.0029 1.0656 1.05 15 1.0800 1.0524 1.0789 0.9804 1.1509 19 04-04-17 1.0654 0.0275 0.0274 0.0028 1.0652 1.0516 l.0791 l .0524 1.0781 0.9800 1.1505 20 05-02-17 1.0691 0.0290 0.0275 0.0028 1.0654 1.0516 1.0794 1.0525 1.0783 0.9802 1.1506 Note: 7-day IC 1s .. 25% Inhibition concentration. An estimation of the sodium chloride concentration that would cause a 25% reduction In Ceriodaphn fo reproduction (calculated using ToMC~ lc) .

CT= Central tendency ol the IC1, values S : Standa rd deviat ion of the 1c ,. values. Control Limits = Mean 1of;larlt hrn c 1C1s +/- 2 stanaarct deviations converted to anti-logarithmic value~ Wai 11i11~ limits = Med11 lo11arlthmlc 1c,. ~ 2CV or s ._ 10 converted to anti-logar thm*c values.

s. 10 ~ Standard deviation cocrespond in to the io** percentile of cvs reported 1mtionally by USEPA(S, 10 = 0.08).

CV = Coeffic len of variat on. -l'* * -- --- - - - - - - Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source : In-house Culture 40 - --- --- --- --- --- ... 35 --- --- --- t: ...... 0 *- * *'--~~~-..---=~~~~~~---~~---------~---__!

  • QJ

*.;:; .,__.,____* ~-:-~~~~~ * --- ---*- u (\1 "C

l 0
0. . . . . 25-E 30 QJ

--. - --- --- --- QJ b.O 0 0:: *= ...

0. 20

... I/) +' ~ c: 0 USEPA Minimum Acceptance Criteria(> 15 offspring per surviving female) o_ u 15 10 40 - c: 0 0 0 USEPA Maximum Coefficient of Variation Guidance Criteria(< 42%), NCDEQ Maximum(< 40%) c: *; ; 0 u +: :l I'll "O 30 *;:: I'll ...c..0 > ....... et: QI 20 0 .....i:::: ...0 C1J ..... i:::: *u 0 10

E u...

<LI --* -.- -. - -.- * --------4----*~--- -------- _,*------ ~.----.....- ..;~-_,* 0 0 .......  !,.. __ - - - - - -- - - -!! - - - --- --- --- u 0 so USE PA Upper PMSD Bound Accept ance Criteria(< 47%) 40 USE PA - '$.. 0 Vl 30 Lower PMSD Bound (>13%) 211. 20 10

  • Control Reproduction, Coefficient of Variat ion (CV) or Percent M inimum Signifi ca nt Difference (PMSD)

PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria . Cent ral Tendency (mean Control Reproduction, CV or PMSD) 95% Confid ence Interval (mean Control Reproduction, CV or PMSD +/- 2 Standard Deviations) Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Source : In-house Culture 0 ~ <o ToxCal Determination Control Re p roduction Control Reproduction CV Tes t PMSO ~ Control Control Reproduction Test (offspring/female) Test num ber Test date Survival Mean CV MSO PMSO CT 95% Confidence Interva l CT '"' 95% Confidence Interval CT 1%) 95% Con fidence Interval (offspring/female) CT+ 25 1 01-12-16 '"' 100 31.2 (%) 3.3 1.3860 (%) 4 .4 31.6 CT

  • 25 28.6 CT + 2S 34.6 4.4 CT* 25 2.1 CT+2S 6.8 5.8 CT
  • 25 43 7 .4 2 02-09-16 100 31.6 7.0 1.9910 6 .3 31.4 28 .8 34.l 4 .6 2.1 7.1 5.9 4 .4 7.4 3 02-09-16 100 32.0 5.9 1.6300 5.1 31.3 29 .0 33.7 4.7 2. 1 7.2 5.8 4 .3 7.3 4 03-08-16 100 29.4 5.4 1.7490 5.9 31.2 28 .7 33.6 4 .7 2.1 7.3 5.8 4 .3 7.3 5 04--05-16 100 29.3 6 .0 1 .7920 6.1 31.l 28.6 33.6 4.8 2. 1 7.4 5.7 4.5 7.0 6 04-05-16 100 32.3 4 .9 1.6950 5 .2 31.2 28.6 33.7 4.8 2.3 7.4 5.7 4.4 6 .9 7 05-10-16 100 31.6 s.o 1.6360 5.2 31.2 28 .8 33.7 4 .8 2 .3 7.4 5.6 4 ,4 6.9 8 06-07-16 100 27.9 2.6 1.7310 6 .2 31.0 28.3 33.7 4.7 2.0 7.3 5.6 4.4 6.9 9 07-12-16 100 32.5 4 .9 1.6710 5.1 31.0 28.3 33.7 4.7 2.1 7.3 5.6 4.4 6.9 10 08-02 -16 100 29.9 6 .6 2.1010 7.0 30.8 28.2 335 4.8 2.2 7.5 5.7 4.3 7.1 11 09* 13-16 100 29.7 5 .9 2.0410 6.9 30.7 28.2 33.3 4.8 2.2 7.5 5.7 4 .2 7.2 12 10-04*16 100 28.9 3.4 1.6720 5 .8 30.5 28.0 33. l 4 .8 2.0 7.5 5.8 4 .3 7 .2 13 11-01-16 100 28.7 4.4 1.8440 6 .4 30.4 27.8 33.0 4 .7 2.0 7.5 5.8 4.3 7.3 14 12-06-16 100 29.3 5 .1 1.4550 5 .0 30.4 27 .7 33 .0 4.8 2.3 7.4 5.8 4 .3 7 .3 15 01-10-17 100 30.5 3 .2 1 .5240 5.0 30.4 27.7 33.0 4.8 2.3 7.4 5.8 4 .3 7.3 16 02-07-17 100 27 .9 8 .5 1.8950 6.8 30.2 27.4 33 .0 5 .1 2.2 8.0 5.9 4 .4 7.4 17 03-07-17 100 29.7 3 .9 1 .5300 5.2 30.2 27.4 33.0 5.1 2.2 8.0 5.8 4 .3 7.4 18 03-07-17 100 29.9 5 .3 1.9180 6 .4 30. 2 27.4 33.l 5.1 2.2 8.0 S.9 4.3 7.4 l!:I 04-04-17 100 30.3 4 .9 1.9240 6.3 30.2 27.4 32.9 5.1 2.2 7.9 5.8 4.3 7.3 20 05-02* 17 100 31.0 5.9 1.8940 6.1 30.2 27 .4 32.9 5.1 2.3 8.0 5.8 4.3 7.3 Nott! : Conirol Survival= USEPA minimum test acceptabilhy criter ia~ 80% survival.

Con ol M~an Reprod\lction = USEPA minimum Jest acceptability criteria~ l5 offsprfng/surv1v1ng female . CV = Coefficient of variat ion fo r control reproduction. USEPA maxlmurr V guida nce criteria (90'" percentile)< 42%. NCOEQ maximum CV acce ptance crlieria < 40%. MSD

  • Minimum gnlllcant difference.

PMSD ~ Percem mlnimun significant diffe rence. PMSO Is a meas1-1e of test precision. The PMSO 1s the minimum pNcent difference between the control and treatment that can be declared stali11i cally significant In a whole effluent toxicity test. lower PMSD bound determined by USEPA (10'" percenlilel > 13%. Th ~ lower PMSO bound re or e.sents a pracUc;il llmtt to th~ sensithtity of the lPs r me1hod ~nn I ~ n.o1 a min1m11m .. rr~ptanrP rrltHia Upner PMSD bound acceptance crlieria determined by USEFA (90'" percentile) < 47~ CT Central t *ndenc~ ot he reproduc1ion, CV or PMSO values. S

  • Standard deviatioo ol the re prod uction. CV or PMSO values.

.I " - c:-:::i ..-::: ' Page 1 of6 Sodium C hlo r id e Chr onic Reference Toxicant Test (EP A-821-R-02-013 M ethod 1002.0) pecies: Ceriodaplz11ia dubia CdNaCJCR #: 201 Dilution preparation information: CommeTtts: NaCl Stock INSS number: INSS \S gc;, Stock preparation: I00 g NaCl/L: Dissolve :50 2 N3CI in 500 mL Milli-Q water. Dil11tinn fll'Cf'I (m2/L) 600 800 1000 1200 1400 Stock volume (ml) 9 12 15 IS 21 Diluent volume (ml) 149 1 1488 14 85 14 82 1479 Tot~I volume (mL) 1500 1500 1500 1500 1500 Test onwnism source i11for111ntio11: Test i11for111ntio11: Organism :ige: I < 24-hours old Randomizi1111, template color: ~ Daie and limes organisms we re born between: I o~*cn*n o'.'\S TI O'\C.O Incubator number and shetr location: 1.&\ Cu lture board: ~-1.* lS*\"'\ ~ Replicate nu mber: I  ? J ; I

  • I s I 6 I 7 f a I 9 I 10 YWT batch:

oc.\*'ll* \"'\ Cullure board cup number: "'!> "-\ I '"'\ I \'O I \ ~ l° I \'\ f'\."\. l't._" J ~ Transfer vessel infomiation: pH= '"'\. l$$ S.U. Temperature= "\S. \ *c Sele11as1rnm batch: Average transfer volume (mL): < 0.25 mL Os.{)\*I\ D"ilY reneivnl i11fo rmatio11 : Day D:it.e Test initfat ion :ind feed ing, MHSW An:llys t renewa l a nd fee din g, or b:itch used termination time 0 05-02-17 0°\\\ o~*1Jo*r1 A ,rt I \J' 05-03-17 ot\"L- o"\.*1.la*\'\ ~ \.-( 2 05-04-17 en'\ O~*U..,

  • l"\ ~ xc*

3 05-05-17 0 2 'U:> o..\* u.-11& ~ 4 05-06-1 7 Ol"l.S l'i.< *n*n }( 5 05--07-17 o&7.:0 c 'St* Q'1.- \"'\ xi" 6 05-08-17 o..t l \ O~*Ol.*\'1 , - X\ ~ 7 05 17 Oil"'l.. - . Control inform.ntiou: Acceptance criteria Summmy of test endpoints:  % of Male Adul ts: ()1. S20% 7-day LC50 .., t'-too  % Adults having 3rd Broods: I rY\1~ 2: 80% NOEC (COO  % Mortality: 01. s20% LO EC t-, /'\('\ Mean Offspring/Female: ~l-0 <!: 15.0 offspri11g/fcmale ChV tl'IC1.S. 5 %CV: <;: .'\ 1. <40.0% IC2s (OC.,'\. l SOP AT14-Exhibit ATl4.I , revision 11-01-14 Page 86of94 ~ ~ p,_ . C'"":J ' l, - p ~ Page 2 of6 Species: Ceriodaplmia dubia CdNaCICR #: 201 CONTROL Survival aurl Reproduction Dnta Replicate number Day I 2 3 4 5 6 7 8 9 IO I Voung protlucctl (} () 0 r-i 0 0 0 () A (') Atlult mortn lity '- '-- L L '--- '-- '-- '-- '-- '- 2 Young protlucctl 0 c () ("\ ri () 0 0 0 0 '- l_ Atl ult morta lity t_ '-- '- '-- '- '- '- '--- 3 \ 'oung produced 0 0 () ("') b 0 0 0 0 0 Atlu!t mortnlity L \..- L L \...... L '- '- '-- '-- 4 Young producctl Yo c; "-\ s "'.\ "-\ lo..\ '-{ s s Adult mormli ty '-- '- '- '- '- L '- '- '-- '-- 5 Young protlucctl Atlult mortality l 't> L \~ ~ \ .,_ '- l\ "-- V"t. L '- "l. ~ L "l. \L ' I\ ""- 10 '- 6 Young protl uccd 0 0 0 0 0 0 u 0 0 0 Adult mortn lity \_ "- \_. '- '--- \..__. L '-- \,.,.. '- 7 Young producctl Total )'Oung producctl \\., ~b ~s \ c:f ~'l. IS ~\ \'\ ~ll) \"" \~ "?..~ \~ 1.~ \ \o \ \.. ~' '°?)"I;) "?>'1. ~1. Finni Adult Mortality '- '- ..,.,... '-- \.._. '- '- \._ '- ~ '- X for J'd Brootls ')(_ )(._ ~ ~ 'X. r-- ")<_ '>£. v Note: Adult mon~lity (I..~ live, D =dead), SB = split broocf (sine lc hrnorl ~ p l i1 he1wecn two dnys}, CO = carry over (offspring carried ov::r with adult during trans fer). Co11ce11tration: 1. 600 muNaCl/L ,.., Survival and Reproduction Data Replica te num ber Day I 2 3 4 5 6 7 8 9 10 I Young produced 0 A 6 6 0 0 0 0 0 0 Adult mortality L \_ L '- L '- L. \.._. L \... 2 Young prol.luccd ("""I n a D (') b 0 a a 0 Adult mortality L. \_ '- ' - '- '- \,_ \.._ '-- '- J Young produced (') r> 0 0 b 0 0 0 0 0 \... L \._ Adult mortality '- '- '- \..- \... \... L .j Young produced 4 5 s s s '-\ s s ~ ',_ '"' Atlult mortality '- \_ '- L L- L- \._ L \.__. L_ 5 Young protluccd Adult mortality 'tL) l.:. L \0 \.._ L \t_ '-* '- IC '1... L L lb l \ L \ I._. \ G Young produced () 0i 0 (') 0 () 0 u ri ('") 1 dull mortali ty \._ '-- '- '-- ~ L.. L L L. '- 7 Young prod uced ,5 >S 11 I~ \'-\ l \c. \S l~ I°& \\a Total young produced (.'\ ~~ ~,_ ~b ~o ~\ ~\ ~~ "'2.C\ ~~ Finni Adult Mortality '- '\_. \.._ L \.._. '-- '- '--- \..__. \._ Note: Ad lllt mortnlity (L = live, D =dead), SB= split brood (single brood split between two days), CO

  • carry over (offspring carried over with adult during transfer).

Co11ce11lratio11:  % Mortality: 01 Mean Offspring/Female: .3l. '2-  % Reduction from Control: -o.toI. SOP AT 14 - Exhib it ATl4 . l, revision l l-Ol - l4 Page 87 of94 l ~ r[=J -- ~ r;:::J Page 3 of6 ,. ~lol1"Milio)W..-..:.""'- Species: Cerio1/ap/111ia dubia CdNaCICR #: 201 800 m!! NaCl/L Survival mu/ Reprod11ctio11 Data Replicate number Day I 2 3 4 5 6 7 8 9 JO I Young produced () 0 0 D b D 0 0 (') 0 Adult mortality '- \..... '- '-- '-- '- '-- ~ '-- L 2 Young produced ('1  !'I b 0 0 0 0 0 0 0 Adult mortality L '- '- \__ \__ '-- '- '- L L 3 Young produced l'J 0 0 CJ {) t) {) 0 0 0 Adult mortality L '-- '- \.._. '- L- '-- '--- L.. '-- 4 Young pr01luccd '-l  ::, 5 s ~ 5 "-\ "-\ '-\ '- '--- '-- ,,_'-- "" \......_ \........

\dull morl:ilit)* L '- \._. '--

5 Young produced \ \. ~'- \\ tD \0 l '?> \ '1.. \ "L \~ Adult mort:ilily l- '- '- L '-- '- '- '- '- '- G Young produced h r'-. ("") {') () 0 0 0 0 0 7 Adult mortality Young produced '-- '~ '- \._ ,~, \ i \_ '- \.\ ,5 '--- '- \::, \..... ,, L L.. '- '-" "' \ \ ' CS) Total young produced  !:lo -- ~t> ,_~ ~\ ~~ 1>>~ ~~ '"l..'\ ~3 Finni Adult i\lorlality '-- \.._ '- '-- '- '-- '-- Note: Ad11Jr monnlity CL = live, D = dead), SB = split brood (single brood split bctween two days). CO = carry over (offspring c~rried over with ad11ll d11ring lrnnsfcr). C011ce11tratio11:  % Mortality: 01. Mean Offsoring/Female: ~.'\  % Reduction from Control: o.~1. 1000 mg NaCI/L Survival mu/ Reproductio11 - Data Replicate number Dny l ~ 3 4 5 6 7 8 9 10 I \loung producctl 0 (\ D 0 n 0 0 0 0 0 Adnll moriali ry \.._. L L.. L L L L '- '- '- 2 \'oung protluccd (") 0 L () L 0 L.. 0 (") L () L a 0 0 Adull mortality '- L \....._ '- L.. 3 Young protluccd (} 0 0 {") 0 0 0 0 0 0 Atluh mortality \...- \.... '- '- '--- \_ \..._ L '-- L 4 Young producctl '"'\ L-- s '.\  ::, ~ '-\ "--\ '-\ \..\ '-l Adult mortality \..- L '- '-- '- L- L \...- '--- 5 Young prollucetl l\ l\ 10 n ... ~o \\ '"L \-,_ 1\ ~ '-- AtluJt morrnlity '-- '- L '-- '-- L \.... \..... L \... G Young producctl n f) (') (', 0 0 0 0 0 0 Atlul! mu rl:ility \... \.- '\_. '- '-- L \..... '- '-- \.... 7 Young pro.ducctl \ :!> \ L. \S \ <; \.~ \ \... \~ I~ 13 'S Tot.al young procluccd "tJi L.'\ u ~\ 1..~ ~\ t.% ~(:) .,_"' 3C finnl ,\dull Mortality L- '-- '-- L L- \.... \_ \....._ \....._ L Note: Aclu ll monalily (L = live, D = dead), SB =splil brood (single brood split between two days). CO= carry over (offspring cnrriecl over with adull during transfer). Co11ce11tratio11:  % Mortalitv:  % Reduction from Control: s. i 1. SOP ATl4 - Exhibit ATl4 . I. revision 11-01-14 Page 88of94 l Page 4 of6 Species: Ceriodaplmia dubia CdNaCICR #: 201 1200 mg NaCl/L Survival n11d Reprod11ctio11 Data Replicate number Day I 2 3 4 5 6 7 8 9 JO I \loung produced () b 0 0 0 () 0 0 0 (') Adult mortality \..... '- '-- '- '-- '-- L \..._ \._ '-

  • 2 Young produced 0 a 0 n 0 0 ('\ 0 a 0 Adult mortality

'- '- '- \.... '- '- '- '- '- '- 3 Young produced 0 0 a 0 ri 0 ri {\ 0 C"I \.._ '- L.. '- '-- \._ 4 Adult morta lity \'oung produced ~ .,_ ,_ ~ ,_ '- '-\ L '-\ '- ~ '- 'b '--\ Adult mortality \.._ \...... '- L.. '- '- '- '- \._ "'-- 5 Young produced ".\ lD c;. s ~ IS 5 \.. ~ '"\ Adult morlality '-- '- \... '- \.... '- '-- '-- '-- \.... G Young produced D 0 0 0 0 0 D 0 0 D Adult mortality \.... \.... '- \... L- \..._. '- '-- L L 7 Young produced .$ \ lo s L.. '4 .'.!> ~ \o \.. \ Totnl young produced \ '4. \ 1.. \S Final Adult Mortality \ "'L '-- " '-- '~ '-- '- '- l Note: Adult mortality CL= live. D =dead}, SB = split brood (single brood split between two days). \ \g '-- \1. '- CO ~ '-- \ 1. '- carry over (offspring \.._ cnrricd over with adult durin; transfer) . Co11ce11tratio11:  % Mo1tality: 0 1. Mean Offspring/Female: \ 1..'L /  % Reduction from Control: (o() .\r:.., . 1400 mg NaCl/L Survival mul Reprorl11ctio11 Data Replicate number Day I 2 3 4 5 G 7 8 9 10 l \'oung produced () 0 0 0 0 0 () 0 0 n Adult mortality L L L.. '-- '- L '-- L '- l 2 Young produced 0 0 (') ("\ ri (\ 0 0 L a ~ Adult mortalil)' '- L L L '- L. \....... '--- '- 3 Young produced ll (\ 0 D 0 (', 0 0 CJ () Adult rnortnlity L \._ '- '-- L. L '- L L \........ 4 Young produced \ L... "'L \ ~ \ ""l. ~ ~ ~ Adult mortality L- '- \._ '- '- \....... L '- '- L. 5 Young produced 0 a\.._. 0 '- 0 \ ("') 0 ("\ 0 Adult mortnli ty \..._ '- '- '- L \.... '- '- '- 6 Young produced 0 f'I () (') b 0 0 ,_ 0 0 Adult mortality '- '- L- '- \...... '--- '- L- L- '-- 7 Young produced "'l.. 0 <::> () 0 D \ () 0 () Totnl yo ung produced ~ L. ..,_ ~ to L. "b s ~ ~ \.._.. L- '- L- '- L \....... '- Final Adult Mortality '- ~ Note: Adult mortality (L =live, D ~ deod}, SB= split brood (single brood split between two days), CO

  • carry over (offspring carried over witl1 adull during transfer) .

Co11ce11tratio11:  % Mortality: 0 1. Mean Offspring/Female: ~~ "2..  % Reduction from Control : 8'1.11. SOP AT14-Ex.hibit ATl4 . l , revision I l*OT-14 Page 89 of94 ~ (Q (I) Verification of Ceriodaphnia Reproduct ion Totals co '\ Q Environm ental Testing Solutions Inc. .... 0 co Cont ro l 1000 mg- NaCl/L ~ Replicate number Replicate number Day Total Day T otal 1 2 3 4 s 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 5 4 4 4 4 5 s 44 4 4 5 4 3 3 4 4 4 4 4 39 5 10 13 12 11 12 12 12 11 11 10 114 5 ll 11 10 12 10 11 12 12 11 12 112 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 15 12 15 14 16 13 l3 16 16 152 7 13 12 15 15 15 16 13 14 13 15 141 To ta l 30 33 34 31 30 32 29 28 32 31 310 To tal 28 28 29 30 28 31 29 30 28 31 292 600 mg- NaCl/L 1200 mg NaCl/L Replicat e num ber Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 I) 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 s 5 4 5 4 5  ; 6 48 4 3 2 2 4 2 4 4 3 3 4 31 5 10 13 10 12 12 10 12 13 11 11 114 5 4 6 5 5 3 8 5 6 3 7 52 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 15 15 ll 13 14 16 15 16 13 16 150 7 5 1 6 5 2 4 3 3 6 4 39 Tot al 29 33 32 30 30 31 31 34 :29 3J 312 To tal 12 9 13 14 7 16 12 12 12 15 122 800 mg NaCl/L 1400 mg NaCl/L - Replicate number Replica t e number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 s 6 7 8 9 10 1 0 0 0 0 0 0 0 0 J 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 J 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 3 5 5 4 5 4 4 4 4 42 4 l 2 2 1 3 l 2 3 3 3 21 5 11 12 1l 10 10 13 12 12 12 13 116 5 0 0 0 2 0 1 0 0 0 0 3 6 0 0 0 0 0 0 0 0 :J 0 0 6 0 0 0 0 3 0 0 2 0 0 5 7 15 14 lL 18 14 15 13 15 17 16 151 7 2 0 0 0 0 0 1 -- 0 0 0 3 Total 30 29 30 33 28 33 29 31 33 33 309 Total 3 2 2 3 6 2 3 5 3 3 32 .......... *r Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Environmental Testing Sol .. tlons. Inc. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test num ber: CdNaCICR 1#201 Test dates: May 02-09, 2017 Concentration Replicate number Surviva l Average reproduction Coefficie nt of Percent reduction (mg/l NaCl) (%) (offspring/female) variation (%) from control (%) l 2 3 4 5 6 7 8 9 10 Control 30 33 34 31 30 32 29 28 32 31 100 31.0 S.9 Not applicable 500 29 33 32 30 30 31 31 34 29 33 100 31.2 S.6 -0.6 800 30 29 30 33 28 33 29 31 33 33 100 30 .9 6.4 0.3 1000 28 28 29 30 28 31 29 30 28 31 100 29.2 4.2 5.8 1200 12 9 13 14 7 16 12 12 12 15 100 12.2 21.8 60.6 1400 3 2 I. 3 6 2 3 5 3 3 100 3.2 41.1 89.7 Ounnett's MSO value : 1.894 MSD = M inimum Significant Difference PMSO: 6. 1 PMSD ~ Percent Minimum Significant Difference PMSO is a measure of test precision . The PMSO i> the minimum percenl difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10'h i;ercentile) = 13%. 1 Upper PMSO bound determined by USE PA (90 h percentile) = 47%. Lower and upper PMSO bounds were determined from the 10th and 90th percent I , respectively, of PMSD data from EPA's WET ln terlaboratory Va riability Study (USE PA, 200la; USE PA, 200lb). The lower PMSO bound represents practical limit 10 the sensitivity of the test method and Is not a minim um cceotance criteria. IJ<;f PA 700111, 200lb. ~in*I Report: lnterliboritory Va rlablllty Study of EPA Short- term Chronic ~nd Atutc Whole Effluent Toxicity Tes1 Ml!thods, Voluml!s 1 and 2* AppendD: . [ PA-821-8-01-004 and EPA-821-11-01-005. US Environmental Protection ARencv, Ci ncinnat i, OH . Statistica l Analyses tnwlronm*fftal THtlnQ SoluUonl. IM. Ceriodaehnia Surv ival and Reeroduction Test-Reeroduction Start Date: 5/212017 Test ID: CdNaCICR Sample ID: R EF-Ref Toxicant End Date: 51912017 Lab ID. ETS -Envir Testing Sol. Sample Type: NACL-Sod1um chloride Sample Date . Protocol* FWCHR-EPA*621

  • R* 02*0 13 Test Specie CD-Cerlodaphnia dvbia Comments:

Conc-m51L 2 3 5 6 7 6 9 10 D-Conlrol 600 2.9 000 30.000 33.000 33.000 34 .000

32. 000

" 31 .000 30.000 30.000 30.000 32 .000 31 000 29.000 31 .000 26.000 34 .000 32.000 29.000 31 000 33000 600 30.000 29.000 30.000 33.000 26.000 33 000 29 000 31 000 33.000 33.000 1000 26000 26.000 29000 30.000 26.000 3 1.000 2 9.000 30 .000 2 6.000 31 000 12.00 12 000 9000 13.000 14.000 7 000 15.000 12 000

  • 2.000 12.000 15 000 140 0 3.000 2.000 2.000 3. 000 6.000 2000 3.000 5 000 3000 3.000 Transform : Untransformed 1-Tailed Isotonic Conc-ma1L Mean N-Mean Mean Min MaJC CV% N  !*Stat Critical MSD Mean N-Mean O.Conlrol Jl 000 1.0000 'H .000 2~.000 34 onn 5 8 11!'1 10 31 .100 1.0000 600 31.200 1 0065 31 .200 29.000 34 .000 5,613 10 *0.241 2.267 1.894 31 .100 1.0000 800 30. 900 0 9968 30 900 28.000 33.000 6 .373 10 0. 121 2.267 1.694 30.900 0 .9936 1000 29.200 0.9419 29.200 26.000 3 1.000 4.210 10 2. 173 2.267 1.894 29.200 0.9369

*1200 12.200 0.3935 12 200 7.000 16.000 21 .790 10 22.694 2.267 1.894 12.200 0.3923 "1 400 3200 0.1032 3.200 2.000 6 .000 41 .143 10 33.557 2. 287 1.894 3.200 0 1029 Auxilia!}'. Tests Statistic Crlllcal Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01 ) 1.00629 1.035 -0. 1735 0 . 13786 Bartlelt's Test tnd1ca les ~uat veriances !E 0.23! = 6.90044 15.0663 H~eothesls Test (1 *tall. 0.05) NOEC LOEC ChV TU MSDu MS Op MSB MSE F-Prob df Dunnett's Test 1000 1200 1095 45 1.89434 0.061 11 1481 .51 3.43 146 3.2E-42 5, 54 Treatments vs 0-Control Linear Interpolation (200 Resample!O Point mgt L so 950/o Cl Skew IC05 959.412 63. 1306 779.216 005.53 -1.2229 IC10 1014 24 8 4 1223 999 237 1023.54 -3.6237 IC15 1032.53 5.7 13 13 1018.69 1041 .09 .() 0750 1.0 JC20 1050.82 5.39734 1036.27 1056.92 -0. 0191 0.9 l C25 1069.12 5.25268 1057 36 1077.82 0 .0038 IC40 1124 5 .8972 1110.95 11 35.01

  • 0.0 715 0.8 ICSO 11 60.59 7 02907 11<15 4 1173.15 - 0.0600 0.7

.,, "' 0 6 ~ o.s ~ 04 "' er:: 0.3 02 0 1 00 c.-----<1-.(Y"" 0 1 -~~~~~~~,....,.~~~~ 0 500 1000 1500 Dose mg/L Dose-Response Plot 40 35 30 1-lail, 0.05 level of significance g u 25

>

15 20 c. ~ 15 10 5 0 ~c. - ... ..__., 0 u 0 Page 92of94 Page 5 o f 6 Species: Ceriotlaplmia dubia CdNaCIC R #: 20 1 Daily Cltemist1y: Day (Ana lyst identified fo r each day. performed. pH. 0 .0. and conductivity measurements only.) 0 l 2 Ana l)*st II- J-.1 .L .;..., ~ Con centration Pa ra meter """'- ~ - . . - r .*, pH (S.U.) 8.2.l 6.o~ S .*o 'b .02. e.oe '\A~ DO (111g/L) "). _q 1-~ ~.'l- ~ "lr ~.e Conductivity . - -- *- ~lct  !" (µmhos/cm) ,32..1 -- 3'2<3 --* - ,_ , "" ..l CONTRO L jl' - - .;, *-*~**-... *Alkalinity (m~CaCO;/L) lo\ '* ... * * .. <j* la"'Z. .-~~ *l ~"'" ' - *lln rd ncss <(g r~I!. g-i. "" *' I (mg CaCOi/Ll . (. "Temperature (0 C) L."\.lo l.C:.. \ "'-\.\."'\ 1...&.\.E "'l.'\. 8 "'\.'\."\ pH (S.U.) t)) 1<; ~-'l\4 e.o'-1 ~~~ {JJ.L)~ ~q\.\ DO {mg/L) "1..8 ~.<3 '\...+ ~-q 1-:~ 1'-8 600 mg NaCl/L Conducth*ity (µmh os/cm ) 14~0 l?AO t3~o .  : " Tem pernture (°C) &.'-\* ""'\ 1..\..\- ~ "'\.'-\*1 '""\.~. f U\*"l 1.'l*~ pH (S.U. ) 0.\"' ~q3 A.01. "iO.-s t!>Cz. 'H\\ DO (mg/L) 'l~ '}.0 '.:\+. ~.'1 -:l':\- 1-.q 800 mg NaCl/L Cond uctivity l1=tQ l":t-0 11-t10 (1m1hos/em) ' - " Tcm pernturc (°C) '1.."-\*\ "\S.O "\_'-\. i "1.~l ."\ 1..'-l* 1. "l.'\ .t pH (S.U.) eJ..\ 3 ~cu e>.oZ '-tAlO A.oz. 1:\...(3 DO (mg/Ll ":l: a l.},A , . *~.a ":\,.q 1:.e'J "t.'l ,_ 1000 mg NaCl/L ~ " Conductivity (µmhos/cm) 2.\30 '-.000 ~05'0 ~ - '* *Temperature (0 C) '1..'-1.*1 "\.J. * .i "l'\.* g 'N."\ -i.'-\* 1 L.'\.f pH (S.U.) e .,z.. '+.<:\( eoa ~~'"' e.oz.. 4 .01.P DO (mg.IL) ~-<3 r.}a ~q ~q ~e 't.~ l 1200 mg NaCl/L Con<luc tivlty (umhos/cm) a4so ~'110 -i.s.o - ~'4.30 1!-i . t , " Tempernt urc (°C) l.."\.1 1.'-\. f "'\.'\ * .f 1.'-1.*'\ ~!\&.{ 0 .12- ~~* ~.01 't.e"' pH (S.U.) 6 .01 j DO (mg/L) e>.o ~<:!\ 0o 8.o '+<'.\' /}~ 1400 mg NaCl/L Con <l udi vi~* (µ mhos/cm ) °'1'10 ~-:t'-ID IA 'l 1<J \ . *Temperature (°C) '"'l"\*1 "1.-.\.. f i.t.1.. e ,,~ . '\ "l'{." \_q.~ J Initial Fina l Initial Fina l Ini tia l F ina l *Temperatures pcrfonncd at the time of test initiation, renewal or tcrminm ion by the analyst idcnti!icd in the Dai ly Renewal lnforniatio'l tablc located on Page I. Alkalinity and~rdncss pcrfonncd by the analyst idcntilic<l on the bench sheet specifi c for cnch analysis and transcribed Lo th is bench sheet by: . *~ SOP AT l4 - ExhibitATl4 . !, revision 11 -0 1- 14 Page 93 of94 'j I I  ; 1~ ~ r= P Page 6 of6 J - .. I Species: Ceriodaplmia dubia CdNaCICR #: 201 l Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 3 4 5 6 l Concentrat ion Ana lyst µ Q..::. """' -. °""!'!. *. .-. .J.-- I)..., . lr' .u .~ ,,_ -- Parameter " ~- - " _:_ -. - ~ pH (S.U.) 0.\3 'iL!>~ ~.o'f, 'l~ ts J'S e.uo. e.\l\ A.\3 1 +_-:}- +.'l 1-.6 r..a.,. ll'l '~.C3 t.A DO (mg/L) Co111Juctivity .- ';/ ,Ci. . ,-::. -~  ;;Tu 32'-l 3ZD --. . - .. (um hos/cm) - - -..----* .31-:r- -* ~ J - .. ~~" -, . -~ .. **- ~.,~" - ~~i'\ """" ~ - ~ ~

  • Alkalinity CONTROL (m~ CaCO,IL) . . . - - '-~ * <1l '

.. -

  • nanlne.ss - 6~ . ,.  :...:;!:~

' ' 86 (mg CaCO;IL) "Temperature - - *- - L.."'-\.l '"l.S.'L. '"\.~. '\ "l.."\ ~ '1.S*\ 1."\.& "\.'$. t> c0 c> ""1..1.\*1 pH (S.U.) A.OS +.Ar "t~ '\Al a.o-6" 0,0\ e,.\\.\ e.o3 DO (mg/L) "'4:~ 1-.'l -:i..'l ~ 1-6 /.\.ll ~.a '\-.c=t - . ~ 600 mg NaCl/L Co n<lucrivity (µmhos/cm) l'260 ~ 14\0 ' *400 - - \~2.0 *-'. '~ . '- *Temperature c0 c> ,_"\~ '1..S.1.. Vo\!\ "\S..O 1.."'.*1 ~4 . .& ).i.\ .'=\ "'l.S.. () pH (S.U.) 0.0-; 1-}\~ i ~'1'- '+~\.\ a.o~ e.o2 0.\3 e.1)2... DO (mg/L) r-l-.'1 ~..o ~ ~.o 'l,,q ".}.~ ~A ~-~ '}*.::t . Con<lucrivity 800 mg NaCl/L ~:f40 (umhos/cm) "'Temperature '1..'-\ *~ 1'1*30 \S.o '1.S-0 l1":20 -- '\.."\..(I-1150 'l~-4'. ,_..\ . ,I ~ -=- C"C) 1..s. o '1.."\* 1 pH (S.U.) e.03 ~A~ "'6.0111 /f?\ I 0.0lP Af\l (2),\3 ~IV> DO (mg/L) ~-nq ~ ,\ ~.c /}J:} I'.\ e ".\-.q '1-.0 g,o 1000 mg NaCl/L Conductivity C1rn1hos/cm) ao~ - .. " - ~uo - a\OO  ;; . " ~l2>0 . . ". "'Tempera tu re '1.c.( * .f -v\. '\ LS.o l,S.Q 1..-\ .f l.."l.'\ "'l."(. ' "'2,.'{. & c*ci pH (S.U.) ,q.02. +.C\<i. "&.o.., 4!10 ~Dt.p '+-.C\Gl 0.l3 1-~q DO (myL) e.o "i :1... 'lSo E!).D ~-q ~o '-t,q e.o a'{(t)O . .. 1200 mg NaCl/L C n<lucti vity ~ .., - - * ~.-. - "t  :"I (~m1hos/cm) ti430 .;\%t> ~'460 "" . "* _ *Temper:itu r c "1$.0 'l.~*f '-~* \ 15.-~ 'lS-1.. °l."l*'i '1...\. '\ ("C) 'lo\ *4' pH (S.U.) ~ ..tn. 1-.'t.~ K~ ~.qo 0.05 6.IY'> e .r~ 'lAe a .* 1400 mg NaCllL DO (mg/L) Con<l ucth<ity B~ o )i1-1o ~.'1... - . +.JI( e?..V - .- - ~o ~.o , ., --; .. ~ Q *O (11mhos/cm) " Temperature 1."l.1 '1.~* g . ~  ;;\'lo.;:, A"i'10  : - "'  ?.6 10 - - ' L.'S. 0 (°C) is.o 1...'i -~ 1.'-l-'\ 1,."\ ...'( 1.'-\.<\ In itia l Fi nal Initial Fina l Initial Final Initial Final "'Temperatures performed at the time of test initiation, renewal or 1crminn1ion by the analyst identified in the Daily Renewal lnfommtion table located on Page I . Alkalinity and hardness performed by the onal)'st identified on the bench sheet spcci fie for each analysis and transcribed to this bench sheet by: ~- SOP AT1 4 - Exhibit AT 14. 1, revision I 1-0 1-1 4 Page 94 of94}}

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