ML17163A414

Submittal of Discharge Monitoring Report for May 2017

ML17163A414
Person / Time
Site:	Sequoyah
Issue date:	06/08/2017
From:	Anthony Williams Tennessee Valley Authority
To:	Document Control Desk, Office of Nuclear Reactor Regulation
References
TN0026450
Download: ML17163A414 (101)

Text

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 8, 2017 Chattanooga Environmental Field Office Division of Water Pollution Control 1301 Riverfront Parkway, #206 Chattanooga, Tennessee 37402-2013 TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR May 2017 Enclosed is the May 2017 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period. The final report for toxicity testing conducted at outfall 101, April 30 - May 5, 2017, is enclosed. No adverse effects were observed. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. ony L. Williams Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _!VA~EQUOYA~UCLEARPLANT _ Addres.L _f. ~BOX.1QOO _ __________ _ _ _ilNTEROFFICE OPS-5N-SQ!!l _______ _ __§ODDY - DAISY.._I.N_273B4_ ____ __ _ _ FacJ!!ly _ _IVA-_fil:QUOYAH NUCLEAR PLANT _____ _ Locatio.!]_ _l!AMIL TOJi.COUNTL_ _________ _ ATIN:M illice nt Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (OMR) TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER AR MO D YE MO DAY ~ r~RING PE~t 1 MAJOR (SUBR 01) F - FINAL DIFFUSER DISCHARGE EFFLUENT

• • • NO DISCHARGE D...

Form Approved. OMB No. 2040-0004 From ; 7 I 05 01 : To [ 17 : 05 I Ji NOTE: Read instructions before completinQ this form. PARAMETER i>< QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 35.6 0 31 / 31 RCORDR 04 CENTIGRADE MEASUREMENT 00010 1 0 PERMIT Req. Mon. DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE 23.4 0 31 I 31 MODELO 04 CENTIGRADE MEASUREMENT 00010 z 0 PERMIT 30.5 DEG.C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE 1.6 0 31 / 31 CALCTD 04 UPSTRM DEG.C MEASUREMENT 00016 1 s PERMIT 3.0 DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 0 31 I 31 RCORDR /908 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT

• • • • • • it*

Req. Mon. MGD CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1433 0 31 I 31 CALCTD TREATMENT PLANT MEASUREMENT 03 03 50050 1 0 PERMIT Req. Mon. MGD MGD CONTI CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE 0.018 0.036 0 20 / 31 GRAB MEASUREMENT 19 50060 1 0 PERMIT 0.1 0.1 MG/L FIVE PER CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MAX WEEK TEMPERATURE - C, RATE OF SAMPLE 0.2 0 31 / 31 CALCTD 62 CHANGE MEASUREMENT 82234 1 0 PERMIT 2.0 DEG CONTI CALCTD EFFLUENT GROSS REQUIREMENT C/HR _,/" ----- ) --------*- NUOUS DAILY MX / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ' ""'""",.,,.,,",_ '"" '" """"'"' '"'., "'""--""'"' "¥. _ / TELEPHONE DATE direction or supervision in accordance with a system designed lo assure thal qualified Anthony L. W illiams personnel properly galher and evaluate the information submilled. Based on my inquiry o the ~ice Pre:dent person or persons who manage the system, or those persons directly responsible for gal ering lhe information, the informalion submilled is, to the best of my knowledge and belief, true, 423 843-7001 17 06 07 Site V ice President accurate, and complele. I am aware that there are significant penalties for submitling false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. T he following injections occurred : Spectrus CT 1300 (max calc. was 0.039 mg/ml, limit 0.05 mg/L), Spectrus BO 1500 (max calc. was 0.046 m gfml, limit - 2.0 mg/ml). EPA Form 3320-1 (REV 3199) Previous editions may be used Pf!OP. 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) Name_~VA~EQUOYA~UCLEA~LANT ___ _ Addres_L _£. ~BOX..£900 - ___.J!NTEROFFICE OPS-5N-SQNl _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMR) TN0026450 101 T MAJOR (SUBR 01) F - FINAL Form Approved. OMB No. 2040-0004 _§ODDY - DAISY J.N_..]7381._ _______ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Fac_ill!y_..JYA-SEQUOYAH NUCLEAR PLANT _____ _ LocatiO.!L _!!AMIL TOJi.COUNTY._ _________ _ ATIN :Millicent Garland PARAMETER QUANTITY OR LOADING AVERAGE MAXIMUM IC25 STATRE 7DAY CHR SAMPLE CERIODAPHNIA MEASUREMENT TRP38 1 0 PERMIT EFFLUENT GROSS REQUIREMENT IC25 STATRE 7DAY CHR SAMPLE PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT EFFLUENT GROSS REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT UNITS MINIMUM >100.0 42.8 MINIMUM >100.0 42.8 MIMINUM DAY 31 EFFLUENT NO DISCHARGE D... NOTE: Read instructions before completini:i this form. QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS 0 1/180 COMPOS 23 PERCENT SEMI COMPOS ANNUAL 0 1/180 COMPOS 23 PERCENT SEMI COMPOS ANNUAL REQUIREMENT / _).. --------_. ~ -~----- NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared un~ direction or supervision in accordance with a system designed to assure that qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry the person or persons who manage the system, or those persons directly responsible for g haring the information, the information submitted is. to the best of my knowledge and belief, tru~. Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled April 30 - May 5, 2017. EPA Form 3320-1 (REV 3/99) Previous editions may be used _/ 1te Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE 423 843-7001 17 06 07 I AREA I NUMBER YEAR MO DAY CODE Pa!'.le 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _!VA~EQUOYA~UCLEARPLANT _ Addres.L_...£. ~BOX 2000 ___________ _ ___.J!.NTEROFFICE OPS-5N-SQ.t:l.l _ ______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) TN0026450 103 G MAJOR (SUBR 01) F - FINAL Form Approved. OMB No. 2040-0004 _ _ _ _§ODDY - DAISY.._lN_]7381_ _______ _ LOW VOL. WASTE TREATMENT POND Facl!l!Y _ _lYA - SEQUOYAH NUCLEAR PLANT _____ _ Location_ _!jAMIL TO Ji.COUNTY..... _________ _ EFFLUENT ATIN:Millicent Garland NO DISCHARGE D... NOTE: Read instructions before completinQ this form. PARAMETER IX QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.2 7.5 0 5 / 31 GRAB MEASUREMENT 12 00400 1 0 PERMIT 6.0 9.0 SU ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 4.5 4.5 0 1 I 31 GRAB MEASUREMENT 19 00530 1 0 PERMIT 30.0 100.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE <5.0 <5.0 0 1 I 31 GRAB MEASUREMENT 19 00556 1 0 PERMIT 15.0 20.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.679 1.757 0 4131 INST AN 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD ONCE/ INST AN EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT / ~q-- --*- NAME/TITLE PRINCIPAL EXECUTIVE OFFICER '°"'~ ~** "'"' °' '~ '"'" '" *oc~"'.,, '".,,~m*** -* """)~ ~. ~. p,.,"."' TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Anthony L. W illiams personnel properly gather and evaluate the information submilled. Based on my inqui of the person or persons who manage the system, or those persons directly responsible fo athering the information, the information submilled is, to the best of my knowledge and beli, true, 423 843-7001 17 06 07 Site V ice President accurate, and complete. I am aware that there are significant penalties for submilli false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99)

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) Name _ _!VA-SEQUOYA~UCLEA~LANT ___ _ Addres_L..f.. ~BOX 20QQ... ___________ _ ___.J.[NTEROFFICE OPS-5N-S(llil _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} DISCHARGE MONITORING REPORT (DMR} TN0026450 110 G MAJOR (SUBR 01) F - FINAL Form Approved. OMB No. 2040-0004 _.§.ODDY - DAISY JN_1738L_ _______ _ Fac.illJY_.JYA - SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ _!!AMIL TOJi..COUNTy_ _________ _ RECYCLED COOLING WATER EFFLUENT ATIN:Millicent Garland ... NO DISCHARGE I xx I... NOTE: Read instructions before completini:i this form. PARAMETER x QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 1 0 PERMIT REPORT EFFLUENT GROSS VALUE REQUIREMENT DAILY MX TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 z 0 PERMIT 30.5 INSTREAM MONITORING REQUIREMENT DAILY MX TEMP. DIFF. BETWEEN SAMP. & SAMPLE UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT 5 EFFLUENT GROSS VALUE REQUIREMENT DAILYMX FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. MGD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 50060 1 0 PERMIT 0.1 0.1 EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILYMX TEMPERATURE - C, RATE OF SAMPLE 04 CHANGE MEASUREMENT 82234 1 0 PERMIT 2 DEGC EFFLUENT GROSS VALUE REQUIREMENT DAILY MX SAMPLE MEASUREMENT PERMIT REQUIREMENT v-- / --j---------- --- NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 'C"'fy ""'" """ * """" '"' *~m'"' '"" "' "'""'~"*-"""""" "(~ direction or supervision in accordance with a syslem designed to assure that qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry the person or persons who manage lhe system, or those persons directly responsible for g thering the information, the information submitted is, to the best of my knowledge and belief, Ir Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used / ~e ice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous CONTIN RCORDR uous 19 MG/L Five per CALCTD Week CONTIN CALCTD uous TELEPHONE DATE 423 843-7001 17 06 07 I AREA I NUMBER YEAR MO DAY CODE Paqe 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name_~~-SEQUOYA~UCLEARPLANT _ AddresL __E. ~BOX.1QOO ___________ _ ___ _J!NTEROFFICE OPS-5N-SQN) _______ _ _.§.ODDY - DAISY JN_j7384_ _______ _ Faclfily_...IVA -_fil:QUOYAH NUCLEAR PLANT _____ _ Location_ _!:!AMIL TOJiCOUNTL ________ _ ATIN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER 1 r~RING p~*~t 1 MAJOR (SUBR 01 ) F *FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I xx I... Form Approved. OMB No. 2040-0004 From v~~* I ~~ : ~~= To Hf ~~ I °tf NOTE: Read instructions before completinQ this form. PARAMETER IX QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE 23 CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERMIT 42.8 PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE 23 PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMIT 42.8 PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT ../,...... ~*--~-- / NAMErrlTLE PRINCIPAL EXECUTIVE OFFICER /__ TELEPHONE DATE ',.. ~ ""'" .. ~.,.

• ~ '"" '"" ""-'"'., """'-" ~"..,... '¥ direction or supervision in accordance with a system designed lo assure Iha! qualified Anthony L. Williams personnel properly gather and evaluate !he information submilled. Based on my inquiry f !he person or persons who manage !he system, or !hose persons directly responsible for g lhering Site V ice President

!he information, !he information submilled is. lo !he bes! of my knowledge and belief, lru accurate, and complete. I am aware Iha! !here are significant penalties for submilling false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Site Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-7001 17 06 07 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _!VA-SEQUOYA~UCLEA~LANT __ _ _ Addres_L _f. ~BOX 2000 ___________ _ ___ _ilNTEROFFICE OPS-5N-SQNl _ _____ _ _ __§.ODD..Y_* DAISY JN~7 3Bi_ _______ _ FacJilly _ _.IVA-_fil:QUOYAH NUCLEAR PLANT ___ _ Locatio..Q_.J:!AMIL TOli_COUNTL _________ _ ATTN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 PERMIT NUMBER 118 G DISCHARGE NUMBER DAY 31 MAJOR (SUBR 01) F - FINAL WASTEWATER & STORM WATER EFFLUENT ... NO DISCHARGE I xx I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinQ this form. PARAMETER IX QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT 19 00300 1 0 PERMIT 2 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1 0 PERMIT 100 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE 25 MEASUREMENT 00545 1 0 PERMIT 1 ML/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MO AVG DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT . --~ / NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under ~P<e,;deot TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Anthony L. W illiams personnel properly gather and evaluate the information submitted. Based on my inqui of the person or persons who manage the system, or those persons directly responsible fot gathering the information, the information submitted is. lo the best of my knowledge and belie true, 423 843-7001 17 06 07 Site Vice President accurate, and complete. I am aware that there are significant penalties for submittin lse i--s1GNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. - OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resu lting from ra infall. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Paae 1 of 1

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: May 24, 2017

1. Facility I Discharger: Sequoyah Nuclear Plant I TVA

2. County I State: Hamilton I Tennessee

3. NPDES Permit#: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1,579

6. Receiving Stream: Tennessee River CTRM 483.6)

7. lQlO: 3,491

8. Outfall Tested: 101

9. Dates Sampled: April 30 - May 05, 2017

10. Average Flow on Days Sampled (MGD): 1834. 1834. 1817 11. Pertinent Site Conditions: Production I operation data will be provided upon request.

12. Test Dates: May 02 - 09, 2017

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested(%):

Pimephales promelas: UV treated Outfall 101: 10.7. 21.4, 42.8. 85.6. 100 UV treated Intake: 1 00 Ceriodaphnia dubia: Non-treated Outfall 101: 10.7. 21.4. 42.8. 85.6. 100 Non-treated Intake: I 00

16. Permit Limit Endpoint(%): Outfall ] 01 : IC2.2. = 42.8%

17. Test Results: Outfall 101 : Pimephales promelas: IC25. > 100%

Ceriodaphnia dubia: lC.25. > 100% Page 1of94

18. Facility

Contact:

Millicent Garland Phone#: (423) 843-6714

19. Consulting I Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone#: (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone#: (256) 386-2787

22. Notes: Exposures to samples collected April 30 - May 05, 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Page 2of94

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall l 01, Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (oC) (mg/L) Last Used Bv 101 04-30-17 0805 to 05-01-17 1332 4.l, 2.2t <0.10 05-02-17 0920 05-01-17 0705 05-03-17 0846 Intake 04-30-17 0710 to 05-01-17 1332 1.7 <0.10 05-02-17 0920 05-01-17 0610 05-03-17 0846 101 05-02-17 0705 to 05-03-17 1235 2.1, l.7t <0.10 05-04-17 0850 05-03-17 0605 05-05-17 0852 Intake 05-02-17 0730 to 05-03-17 1235 2.2 <0.10 05-04-17 0850 05-03-17 0630 05-05-17 0852 05-04-17 0730 to 05-06-17 0930 101 05-05-17 1230 1.1, l.3t <0.10 05-07-17 0952 05-05-17 0630 05-08-17 0833 05-04-17 0745 to 05-06-17 0930 Intake 05-05-17 1230 0.9 <0.10 05-07-17 0952 . 05-05-17 0645 05-08-17 0833

• TRC = Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- l.0°C) in a warm water bath.

Page 3of94 Aliquots of Outfall l 0 l and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Pimephales promelas Test Organisms:

1. Source:

In-house Cultures

2. Age:

< 24-hours old Test Method Summary:

l. Test Conditions:

Static, Renewal

2. Test Duration:

3. Control I Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

1

5. Organisms per Replicate: lQ

6. Test Initiation: (Date/Time): 05-02-17 0832 ET

7. Test Termination: (Date/Time): 05-09-17 0857 ET

8. Test Temperature: Outfall 101:

Mean = 24.7°C (24.2 - 25.1°C)

9. Physical I Chemical Ceriodaphnia dubia In-house Cultures

< 24-hours old Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic l 05-02-17 0920 ET 05-09-17 0827 ET Mean = 24.9°C (24.6 - 25.2°C) Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each I 00% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Page 4of94 Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 02 - 09, 2017 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7

Control, 100 100 100 100 100 100 100 UV-treated 10.7%

100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98

Control, 100 100 100 100 100 100 98 Non-treated Test Solutions Mean Dry Weight (mg)

(replicate number) (%Effluent) 1 2 3 4 Mean

Control, 0.632 0.723 0.732 0.703 0.698 UV-treated 10.7%

0.700 0.704 0.701 0.677 0.696 21.4% 0.730 0.701 0.689 0.771 0.723 42.8% 0.687 0.642 0.662 0.684 0.669 85.6% 0.633 0.719 0.657 0.666 0.669 100.0% 0.644 0.649 0.627 0.637 0.639 Intake 0.650 0.605 0.596 0.688 0.635

Control, 0.632 0.783 0.630 0.669 0.679 Non-treated IC2s Value: > 100%

Calculated TU Estimates: < 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

• TUa = 100/LCso: TUc = 100/ IC2s Page 5of94

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 02 - 09, 2017 using effluent from Outfall 101. Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100. JOO Test Solutions Reproduction (#young/female/7 days) Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 33 28 30 32 35 30 32 30 32 31.2 10.7% 34 36 31 34 33 31 32 35 31 31 32.8 21.4% 37 36 31 33 32 34 31 33 32 37 33.6 42.8% 35 36 37 32 33 33 35 34 36 33 34.4 85.6% 37 35 34 38 32 38 38 36 39 32 35.9 100.0% 38 36 37 36 37 39 39 35 38 33 36.8 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

• TUa = 100/LC5o: TUc = 100/ IC2s Page 6of94

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 02 - 09, 2017 using water from Intake Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effiuent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effiuent) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 33 31 32 34 34 31 28 32 30 31 31.6 Intake 38 34 36 37 35 31 37 32 34 32 34.6 IC2s Value: > I 00% Calculated TU Estimates: < 1.0 TUc* Permit Limit: NI A Permit Limit: NI A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

• TUa = 100/LC5o: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC2s) Pimephales promelas May 02 - 09, 2017 0820 7-days KCI 0.67 g/L Ceriodaphnia dubia May 02 - 09, 2017 0911 7-days NaCl 1.07 g/L Page 7 of94

~ PBYSICALJCHEMICAL

SUMMARY

CQ Water Chemistry Mean Values and Ranges fi>r UV-treated Pimephales promelas and Non-treated Ceriodaphnia dubia, Sequoyah Nuclear Plant (SQN), Efiluent Outfiill 10 l and Intake CD perfi>nned May 02-09, 2017. ClO 0.... ~ Test Sample ID Temperature ("C) Dissolved Oxn:en (llll?fL) Initial Final Initial Final

Control, 24.7 24.5 7.8 7.0 Non-treated 24.6 - 24.8 24.3 - 24.8 7.7 - 7.9 6.0 -

7.8

Control, 24.7 24.4 8.0 7.1 UV-treated 24.6 - 24.8 24.2 -

24.7 7.9 - 8.2 6.2 - 7.7 ~ 10.7% 24.8 24.6 8.0 7.1 ~ 24.7 - 24.9 24.4 - 24.9 7.9 - 8.1 6.2 - 7.7 i: 21.4% 24.9 24.5 8.0 7.0 24.7 - 25.0 24.3 - 24.7 7.9 - 8.2 6.1 - 7.7 ~ 24.9 24.6 8.0 6.9 42.8% --= -~ 24.8 - 25.0 24.2 - 24.9 7.9 - 8.1 6.0 - 7.7 85.6% 25.0 24.6 8.0 6.9 Q., 24.9 - 25.1 24.2 - 24.9 7.9 - 8.1 6.0 - 7.7 100% 25.0 24.6 8.0 6.9 24.9 - 25.1 24.2 - 24.8 7.9 - 8.1 5.9 - 7.7 Intake 25.0 24.6 8.0 7.0 24.9 - 25.l 24.4 - 24.9 7.9 - 8.1 5.9 - 7.7

Control, 24.7 25.0 7.8 7.9 Non-treated 24.6 - 24.8 24.9 - 25.2 7.7 - 7.9 7.8 - 8.0 10.7%

24.8 25.0 7.8 7.9 24.7 - 24.9 24.7 - 25.2 7.8 - 7.9 7.7 - 8.0 .s 24.8 25.0 7.8 7.9 21.4% .§ 24.7 - 24.9 24.8 - 25.2 7.8 - 8.0 7.8 - 8.0 .s 24.8 24.9 7.9 7.9 =: 42.8% --=.§' 24.7 - 24.9 24.8 - 25.0 7.8 - 8.0 7.8 - 8.0

• ~

85.6% 24.9 24.9 7.9 8.0 ~ 24.7 - 25.0 24.8 - 25.1 7.8 - 8.0 7.9 - 8.1 100% 24.9 24.9 8.0 8.0 24.7 - 25.0 24.8 - 25.2 7.9 - 8.0 7.9 - 8.2 Intake 24.9 24.9 8.0 8.0 24.7 - 25.0 24.8 - 25.0 7.9 - 8.1 7.8 - 8.2

• Note: Total residual chlorine was perfonned on non-treated Outfall 101 and Intake samples.

Overall temperature ("C) Pimephales prome/as Ceriodaphnia dubia Average 24.7 24.9 Minimum 24.2 24.6 Maximum 25.l 25.2 pH~S.U.) Initial Final 8.13 7.69 8.08 - 8.21 7.49 - 7.92 8.09 7.72 8.05 - 8.19 7.55 - 7.93 8.11 7.67 8.06 - 8.18 7.49 - 7.89 8.11 7.67 8.04 - 8.17 7.48 - 7.87 8.09 7.67 7.98 - 8.18 7.49 - 7.87 8.09 7.67 7.99 - 8.19 7.49 - 7.86 8.08 7.65 8.03 - 8.19 7.48 - 7.87 8.09 7.69 8.03 - 8.18 7.48 - 7.91 8.13 8.03 8.08 - 8.21 7.97 - 8.09 8.12 8.02 8.06 - 8.18 7.97 - 8.09 8.13 8.01 8.09 - 8.19 7.97 - 8.08 8.12 8.02 8.08 - 8.19 7.97 - 8.09 8.12 8.02 8.08 - 8.19 7.97 - 8.10 8.08 8.01 7.95 - 8.19 7.92 - 8.09 8.09 8.04 8.02 - 8.18 7.98 - 8.11 Conductance Alkalinity Hanlness

• Total Residual

(µmhos/cm) (mg/L CaC03) (mg/L CaC03) Chlorine (mg/L) 322 62 85 317 - 328 61 - 63 82 88 320 62 87 312 - 326 61 - 64 86 88 307 295 - 318 294 283 - 305 259 250 - 267 190 186 - 193 165 64 65 < 0.10 160 - 169 59 - 69 64 - 66 < 0.10 - < 0.10 165 64 67 < 0.10 158 - 170 61 - 67 64 70 < 0.10 - < 0.10 322 62 85 317 - 328 61 - 63 82 - 88 308 298 - 316 292 286 - 300 258 248 - 265 188 182 - 196 164 63 65 <0.10 158 - 171 59 - 67 62 - 66 < 0.10 - < 0.10 166 64 66 < 0.10 162 - 177 59 - 67 64 - 68 < 0.10 - < 0.10

SUMMARY

I CONCLUSIONS Exposures to samples collected April 30- May 05, 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Page 9of94

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction oftest organisms. UV treatment is used to control interference of fish pathogens. This treatment method was approved on November 23, 2015 by the State of Tennessee in a letter from Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring.

2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS I. Pimephales promelas None

2. Ceriodaphnia dubia None Page 10of94

PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B-2010.

4. Dissolved oxygen was measured by SM 4500-0 G-20 I 1.

5. The pH was measured by SM 4500-H+ B-2011.

6. Conductance was measured by SM 2510 B-2011.

7. Alkalinity was measured by SM 2320 B-2011.

8. Total hardness was measured by SM 2340 C-2011.

9. Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC2s values in g/L KCI or NaCl.

2.

Standard Toxicant: Potassium Chloride (KC! crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses. Page 11of94

REFERENCES l. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-82l-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 12of94

Page 13of94 Sequoyah Nuclear Plant Biomonitoring May 02 - 09, 2017 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 2017 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L TRC EO/PO 02/06/2005 <0.0042 0.028 0.010 02/07/2005 <0.0116 0.028 0.010 0.007 02/08/2005 <0.0080 0.028 0.010 02/09/2005 0.0199 0.028 0.010 02/10/2005 <0.0042 0.028 0.010 02/1112005 0.0155 0.028 0.010 0.007 0610512005 0.0063 0610612005 0.0043 0610712005 0.0103 06/08/2005 0.0295 0610912005 0.0129 06/10/2005 0.0184 07117/2005 0.0109 0.026 0.009 07/18/2005 0.0150 0.026 0.009 0711912005 0.0163 0.026 0.009 07/20/2005 0.0209 0.026 0.009 0.014 07/21/2005 0.0242 0.026 0.009 07/22/2005 0.0238 0.054 0.018 0.014 I 0/30/2005 0.0068 10/3112005 0.0112 11 /01/2005 0.0104 11 /02/2005 0.0104 11/03/2005 0.0117 11/04/2005 0.0165 11/14/2005 0.0274 11115/2005 0.0256 11/16/2005 0.0234 11117/2005 0.0231 11/18/2005 0.0200 11119/2005 0.0116 Page 14of94 H-150M mg/L Quat 0.037 0.037 0.037 0.036 0.036 0.036 0.035 0.036 0.036 0.035

Date 11 /12/2006 11 /13/2006 11/14/2006 11/15/2006 11/16/2006 11/17/2006 11/26/2006 11/27/2006 11/28/2006 11129/2006 11 /30/2006 12/01/2006 05/28/07 05129107 05/30/07 05/31 /07 06101107 06102107 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04/15/08 04/16/08 04117108 04/18/08 I 0/26/08 10/27/08 10/28/08 10/29/08 I 0/30/08 10/31/08 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 2017 Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L TRC EO/PO 0.0055 0.0068 0.0143 0.0068 0.0267 0.0222 0.0188 0.0138 0.0120 0.0288 0.0376 0.0187 0.0084 0.017 0.0103 0.0164 0.017 0.0305 0.0241 0.0128 0.0238 0.0158 0.0162 0.0175 0.0039 0.0124 0.0229 0.0143 0.0120 0.0149 0.0260 0.0151 0.017 0.0172 0.0154 0.018 0.0086 Page 15 of 94 H-150M MSW mg/L 101 Quat mg/L Phosphate 0.037 0.037 0.037 0.037 0.015 0.036 0.015 0.036 0.015 0.036 0.015 0.036 0.015 0.015 0.041 0.041 0.030 0.041 0.030 0.041 0.030

Date 02/08/09 02/09/09 02/10/09 02/1 1/09 0211 2/09 02/1 3/09 05110109 05111109 05/12/09 0511 3109 0511 4109 0511 5109 11115109 11116109 11 /1 7/09 11 /1 8/09 11 /1 9/09 11120109 0510911 0 05/ 10/ 10 0511 111 0 05/12/1 0 05/13/1 0 05/14/1 0 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Micro biologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 2017 Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg!L TRC Phosph EO/PO Quat ate 0.0197 0.017 0.0237 0.017 0.0104 0.021 0.0155 0.017 0.0106 0.017 0.0129 0.0415 0.0053 0.0049 <0.0141 <0.0160 0.025 0.0152 0.0255 0.0306 0.0204 0.0093 0.0192 0.0055 0.0100 0.039 0.0171 0.039 0.0041 0.039 0.0099 0.039 Page 16of94 H-150M MSW mg/L 101 Quat mg!L Phosphate 0.0446 0.0396 0.0396 0.0397

Date 10/31/IO I I /01/1 0 11/02/10 11 /03/1 0 I 1/0411 0 11/05/10 05/01 /2011 05/02/2011 05103120 I I 05/04/2011 0510512011 05/06/2011 l l/06/201 I 11 /07/2011 11 /08/2011 11 /09/2011 11/] 0/2011 11 /J 1/2011 05/06/201 2 05/07/201 2 05/08/201 2 05/09/201 2 05/10/201 2 05/J 1/201 2 08/J 2/201 2 08/13/201 2 08/14/201 2 08/J 5/201 2 08/J 6/201 2 0811 7/201 2 05/12/2013 051131201 3 05/1 4/201 3 05115/201 3 05/1 6/201 3 05/17/201 3 09/15/201 3 0911 6/20 I 3 09/1 7/201 3 09/18/201 3 09/19/201 3 09/20/201 3 Table B-1. Sequoyah Nuclear Plant Outfall l 01 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 2017 Sodium Towerbrom PCL-PCL-401 CL-363 Cu pro stat H-130M Nalco Spectrus H-150M Hypo-mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L chlorite TRC mg/L Copoiy-DMAD Azoie Quat mg/L mg/L Quat mg/L Phos-mer EO/PO Quat TRC oh ate 0.01 22 0.0112 0.0163 0.0107 0.01 32 0.04 0.04 0.0 155 0.04 0.0179 0.04 0.0089 0.0168 0.0225 0.01 41 0.0239 0.0242 0.0231 0.041 0.0145 0.041 0.0298 0.041 0.0174 0.0256 0.028 0.037 0.0209 0.037 0.0279 0.028 0.0076 0.0446 0.0099 0.0091 0.039 0.0096 0.039 0.0229 0.0063 0.0072 0.0379 0.0107 0.036 0.0379 0.0217 0.036 0.0379 0.0 172 0.01 73 Page 17of94 MSW Fioguard 101 MS6236 mg/L mg/L Phos-Phosphate oh ate 0.029 0.029 0.029 0.029 0.029 0.032 0.064 0.064 0.064 0.032 0.032 0.03 0.03 0.03 0.03 0.03 0.03

Date 05/04/2014 05/05/20 14 05/06/2014 05/07/2014 05/08/20 14 05/09/20 14 09/07/20 14 09/08/2014 09/09/2014 0911 0/2014 09/11 /2014 091121201 4 08/09/2015 08/J 0/2015 08/1 1/2015 08/1 2/20 15 08/13/201 5 08/\\ 4/20 15 I 0/18/2015 I 0/1 9/20 15 10/20/2015 10/21/201 5 10/22/201 5 10/23/20 15 0511 5/2016 0511 6120 16 05/1 7/20 16 05/18/2016 05/1 9/2016 05/20/2016 07131/2016 08/01/20 16 08/02/20 16 08/03/201 6 08/04/20 16 08/05/20 16 04/30/20 17 05/01 /20 17 05/02/20 17 05/03/2017 05/04/20 17 05/05/20 17 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 5, 201 7 Sodium Towerbrom PCL-PCL-401 CL-363 Cu pro stat H-130M Nalco Spectrus H-150M Hypo-mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CTl300 mg/L chlorite TRC mg/L Co poly-DMAD Azole Quat mg/L mg/L Quat mg/L Phos-mer EO/PO Quat TRC phate 0.0118 0.0112 0.0096 0.0164 0.0235 0.0110 0.04 0.04 0.04 0.0070 0.0074 0.0195 0.03 0.0275 0.03 0.0213 0.0192 0.0182 0.0162 0.0125 0.0120 0.0130 0.0174 0.0156 0.0209 0.0210 0.0361 0.0254 0.0261 0.0091 0.03 0.0093 0.03 0.0209 0.03 0.0298 0.0218 0.0260 Page 18of94 MSW Floguard 101 MS6236 mg/L mg/L Phos-Phosphate phate 0.03 0.03 0.03

Page 19of94 Sequoyah Nuclear Plant Biomonitoring May 02 - 09, 2017 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

BIOMONITOIUNG CHAIN OF CUSTODY RECORD Page~l~of~l~ ~ en t: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): I Q) FedEx UPS Bus Client oject Name: Sequoyah NP Toxicity 3 51 Depot Street. l'1 Asheville, NC SoV'\\I Q_ b (:/,'verj ~. Number: N/ A Other (specify): 0 ~ility Sampled: Sequoyah NP 28801 General Comments: "/00,e Sor-ill~ /)0 1-> /.JJ/ f~J>l~re. "7.50 m-. 5-/-/? NPDES Number: TN0026450 Phone: 828-350-9364 Too.t ~f'ks fr-o~ ~,£ !Aflue_e>7.Jot?;.S-l*f/ ~wtJ.~re ~~' Fax: 828-350-9368 Collected By 0 ~ 1' o;.s.s. Jf'?-e!J µe.,,re C!Pll-edeJ,t- _kej>I ~~He_ 8rv5o-->V~ ~'1 /IL v \\ I i)l\\.DJ ed'~ I 23az. Container Field Identification I Number& Flow Rain Event? Sample Description Grab/Comp. Collection Datetrime Volume (MGD) Laboratory Use Collected (Mark as Appropriate) -~;*.*

• )*:O * --

\\""" "(\\, :, -:.. If Yes, ETS Log Arrival Temp. By Time Appear*

• ,*.L..

Start End ~ r.- ~-. Yes No Trace Number ("C} ... ",' __.~ ~:*..)_~ *.. .,,..- {.;,/ ~ Inches E\\ an cc 'l-30-/7 5-/-/7 1 Y33.q;J. x 0.51 ~.03 c.l.\\ \\ '2..1.:L ~ \\?]:>'- SQN-101-TOX Comp ~'io~ ~ ()'7C>S-.Ex 2(2.Sgal) -A SQN-INT-TOX Comp l/*JD-17 s- /-17 1(2.5 gal) A/14 x o.51 \\~. o'i \\.1*c_ ~ \\g,1..- .f. 07/D~ Ob/D ~ Sample Custody - Fill In From Top Down -is ('.i<"t"Ot:$4 C"o-"\\.< 1"'1'iAr~ <.A~fLe<) Relinquished By (Signature): Date/Time Received By (Signature): ~,\\)th 1tl <l-OOb C..0~\\?l\\'t"lOM. ~C.. ~~ \\~ A\\l... S".-\\P\\tS Oat~~tme lnL vd //y//l 5=!-17 IO?!~ ~81(2£L SOt..l\\(.. tJS--- 0/-1? / o<e.*) £T (....- v / So~:ac_ - tSI /3.3\\.. ~' I E~ / Bl( ~ 0~'- 1-tr"J (' ~ O~

• O\\- 1"\\

\\~~1-. Er Instructions: Clients should fill in all areas except those in the "Laboratory Use'* block. Biomonitoring samples are preserved by storing them al 6°C and shipping them in ice. The hold time for each sample is 36 hou rs from the time of collection. Therefore. please collecl and ship in such a way that the laboratory wil receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Whole Effluent Sample Receipt Log Page 52

• sample temperature performed using Sample Receivitlll Thermometer: SN 160928622 Date Time Received Received
• sample Project Sample Sample name and description State Comments Received Received by from Temp. (

0C) number number 05-01-17 1245 J. Sumner

l. Keenan 4.1 12330 170501.01 Jefferson WWTP NC I

05-01-17 1245 I. Sum.,er

l. Keenan 4.1 12331 170501.02 West Jefferson WWTP NC I

05-01-17 1332 J. Sumner TVA Courier 4.1/2.2 12332 170501.03 TVA - Sequoyah Nuclear Plant - 101 TN I 05-01-17 1332 J. Sumner TVA Courier 1.7 12332 170501.04 TVA - Sequoyah Nuclear Plant - Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12

BIOMONITORING CHAIN OF CUSTODY RECORD Page _l_ of _1_ 'tjent: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): I FedEx UPS Bus Client 'fioject Name: Sequoyah NP Toxicity 351 Depot Street. ~* Number: N/ A Asheville, NC Other (specify): 52.....,,*c_ Del<<uer'y &ility Sampled: Sequoyah NP 28801 General Comments(' 'bv,,<< 3'cc~lef 1r~ It:!_ 5~;.-i,P/c-/" (!! P7,y; on S: J'-1-; NPDES Number: TN0026450 Phone: 828-350-9364 1(;0.e:_ ~f'ef lro,...,_1.,..f!. s~-e_o~ b>'ls~3- /,7 /P<>/U-S tJ>_,.,,,,,, "-- f.(""'1 ~ Fax: 828-350-9368 Collected By :~~ j~ -x Oc'15.riM- ~e d~./ r /~,.La AX'C._ Container f fUJj ~ l 2 3 3 '2. Field Identification I Number& Flow Rain Event? Sample Description Grab/Comp. Collection Dateffime Volume (MOD) Laboratory Use Collected (Mark as Appropriate) r<"' .. 11;__;/_ r ~:i-*. *. - .:..-.:\\*--c-~.,-;.. *i ~. ~ If Yes. ETS Log Arrival Temp. By Time Appear-

• .: ___ *~;.;.Ii:~:{;.:

,:.~*-**... ~-... :-r? -~- ),

• Stan End

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:.1'!'l *

/~ 't,:',7 ?.. ~ Yes Inches No Trace Number (°C) e.~ Ao ..,... 'le...:' l"':.!- an cc s-~ -/7 S-3-17 13'33.?i I l-::Ja503~-S -i. \\ \\ \\,\\ *c_ ~ ~1j<., 1t SQN-101-TOX Comp IJ'705Ei otoDS-e.' 2(2.5gal) 5-2-1 7 S-3-/7 1(2.5 gal) NA x '109)3.~ t..1.: (.. ~ ""';S >rr-SQN-INT-TOX Comp 07:?0 1 t>IP3DE' Sample Custody - Fill in From Top Down ~ <:..\\)~~\\:)'\\ ~ 1..n-11(...Ji"". $~(6 Relinquished By (Signature). Date/Time Received By (Signature): ~<...e\\'-lt.b \\~ 6-ion (.O~~\\TlON. \\l\\L ate/Time A~Sb.J\\ ii>...} A\\...'. I k/~A/---/ p~.5-3-11/ot1& e:~ /3 R __H~ SON\\(_ O§vcJJ.... /1 ~* '.... '--.;-_ ~. d\\ o<i/'C E\\ v / ) ~ J'r_ '5o~L of'~Ol*t) 12:) f' E.:'" r '1Mt. / E~ o.s- <r'::>- I\\ \\l._)5 E\\ v Instructions: Clients should fill in all areas except those in the *'Laboratory Use** block. Biomonitoring samples arc preserved by storing them at 6°C and shipping them in ice. TI1e hold time for each sample is 36 hours from the time of collection. Therefore. please collect and ship in *uch a way that the laboratory will receive the samples with ample lime to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS musr. be marked for Saturday delivery or they will not arrive until the following Monday.

Whole Effluent Sample Receipt Log Page~

• sample temperature performed using Sample Receiving Thermometer: SN 160928622 Date Time Received Received
• sample Project Sample Sample name and description State Comments Received Received by from Temp. (0C) number number 05-03-17 0910 K. Keenan Fed - Ex 0.7 12346 170503.01 Rockingham WWTP NC 05-03-17 0950 K. Keenan Fed - Ex 1.0 12347 170503.02 ApexWRF NC 05-03-17 0950 K. Keenan Fed - Ex 1.2 12348 170503.03 Belews Creek SS NC 05-03-17 0950 K. Keenan Fed
• Ex 4.1 12349 170503.04 Daikin Applied Americas NC 05-03-17 0950 K. Keenan Fed - Ex 0.3 12350 170503.OS Dan River CC NC 05-03-17 0950 K. Keenan Fed - Ex 2.4 12351 170503.06 Davidson Water WTP NC 05-03-17 0950 K. Keenan Fed
• Ex 0.8 12352 170503.07 Dobson WTP NC 05-03-17 0950 K. Keenan Fed
• Ex 1.2 12353 170503.08 James Loughlin WWTP NC 05-03-17 0950 K. Keenan Fed
• Ex 0.8 12354 170503.09 Performance Fibers NC 05-03-17 0950 K. Keenan Fed
• Ex 1.2 12355 170503.1 0 Roseboro WWTP NC 05-03-17 0950 K. Keenan Fed - Ex 0.3 12356 170503. I I Scarlett Acres MHP NC 05-03-17 0950 K. Keenan fed - Ex 0.8 12357 170503. 12 Sparks Road WTP NC 05-03-17 0950 K. Keenan Fed
• Ex 2.0 12358 170503.13 Elementis NC 05-03-1/

0948 K.Keenan UPS 10.5 12359 170503.14 Morehead City WWTP NC Exceede:J Temp. requirement 05-03-17 0950 K. Keenan Fed - Ex 1.7 12360 170503.15 AQWA NC 05-03*17 0950 K. Keenan Fed - Ex 1.7 12361 170503.16 Bladen Bluffs WTP NC 05-03-17 0950 K. Keenan Fed - Ex 1.7 12362 170503.17 Buckeye Terminals NC 05-03-17 0950 K. Keenan Fed - Ex 1.2 12363 170503.18 lnvisita

• 001 NC 05-03-17 0950 K. Keenan Fed - Ex 1.2 12364 170503.19 lnvisita
• 002 NC 05-03-17 0950 K. Keenan Fed
• Ex 0.1 12365 170503.20 ManteoWWTP NC 05-03-17 0950 K. Keenan Fed - Ex 0.3 12366 170503.21 Mayo Stream Electric Plant NC 05-03-17 0950 K. Keenan Fed - Ex 1.2 12367 170503.22 Pender County WTP NC 05-03-17 0950 K. Keenan Fed
• Ex 1.3 12368 170503.23 Shearon Harris Plant NC 05*03-17 0950 K. Keenan Fed - Ex l.5 12369 170503.24 Shearon Harris E&E Center NC 05-03-17 095Q K.Keenan Fed - Ex 1.0 12370 170503.25 Washington WWTP NC 05-03-17 0950 K. Keenan Fed* Ex 1.2 12371 170503.26 PCS Phosphate, Inc.

NC 05-03-17 0950 K. Keenan Fed* Ex 0.3 12372 170503.27 Woodlake Yacht Club NC OS-03-17 0950 K. Keenan Fed - Ex 0.3 12373 170503.28 TVA

• Allen Fossil Plant - 001 TN 05*03-17 0950 K. Keenan Fed
• Ex 0.2 12373 170503.29 TVA
• Allen Fossil Plant - Intake TN 05-03-17 0950 K. Keenan Fed* Ex 0.1 12373 170503.30 TVA - Allen Fossil Plant - Upstream TN 05-03-17 0950 K. Keenan Fed - Ex 1.2 12374 170503.31 Carolina Beach WWTP NC 05-03-17 1152 J. Sumner Dash Courier 0.3 12375 170503.32 OWASA
• Mason Farms WWT-:>

NC 05-03-17 1215 K. Keenan L. Keenan 4.0 12330 170503.33 Jefferson WWTP NC 05-03-17 1215 K. Keenan L. Keenan 4.0 12331 170503.34 West Jefferson WWTP NC 05-03-17 1235 I. Sumner TVA Courier 2.1/1.7 12332 170503.35 TVA

• Sequoyah Nuclear Plant-101 TN 05-03-17 1235 J. Sumner TVA Courier 2.2 12332 170503.36 TVA - Sequoyah Nuclear Plant* Intake TN SOP G4
• Exhibit G4.2, revision 01-03-12

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m the*'41Abl>Ta(Qry l!~" bloc:~ Ji!nDtOnlt~.1 santplctt.ate :J1~.vt:d by Sltldnl them at b'C D?<l, ~lpp,lng tbem '" kc. '.f'h1t hbkl time for ttcb ~mp'lc . le~~ 6:om dtc time Of ~lc~on. TI{eteRln. plea. c:Oll~ and khip In ~~Ira Wf/i <<mt t114 J.iio~ory-will receive tllC ~ with amplt rime to1TI1hiµc t~ whhln that. time fra111e *. $1mpl~ sb1ppe,d . ovt;trughl Qn.Ftlday 'i'ia FlldBx.'Qr UMJ must be RU!tkad fbr 68turdll.Y.dettvtry or~ Wlf I not 1ltl'fVC unall the following Mond'ay.

Whole Effluent Sample Receipt log Page Sta 'Sample 1emperature performed uslne Sample Receiving Thermome1er: SN 1609286~2 Date Time Received Received

• Sample Project Sample Sample name and description State Comments Received Received by from Temp. (°C) number number 05-05-17 0943 K. Keenan Fed - Ex 0.9 12347 170505.01 Apex WRF NC 05-05-17 0943 K. Keenan fed* Ex 0.1 12348 170505.02 Belews Creek SS NC 05-05-17 0943 K.Keenan Fed - Ex 3.3 12349 170505.03 Daikin Applied Americas NC 05-05-17 0943 K. Keenan Fed* Ex 0.8 12350 170505.04 Dan River CC NC 05-05*17 0943 K. Keenan Fed - Ex 2.1 12351 170505.05 Davidson Water WTP NC 05-05-17 0943 K.Keenan Fed - Ex 0.3 12352 170505.06 DobsonWTP NC 05-05-17 0943 K. Keenan Fed - Ex 0.9 12353 170505.07 James Loughlin WWTP NC 05-05-17 0943 K.Keenan Fed - Ex 0.3 12354 170505.08 Performance Fibers NC 05-05*17 0943 K. Keenan Fed - Ex 0.4 12355 170505.09 Roseboro WWTP NC 05*05-17 0943 K. Keenan Fed - Ex 0.2 12356 170505. 10 Scarlett Acres MHP NC 05-05-17 0943 K. Keenan Fed - Ex 0.3 12357 170505. 11 Spa(ks Road WTP NC 05-05-17 0943 K. Keenan Fed - Ex 0.7 12358 170505. 12 Elementis NC 05-05-17 1220 K. Keenan Dash Courier 0.4 12375 170505.13 OWASA-Mason Farms WWTP NC 05-0!>-17 1220 K. Keenan Dash Courier 0.7 12346 170505. 14 Rockingham WWTP NC 05-05-17 1240 K. Keenan J. Brewer 1.4 12330 170505. 15 Jefferson WWTP NC 05-05-17 1240 K. Keenan J, Brewer 2.4 12331 17050S.16 West Jefferson WWTP NC 05-05-17 1230 J. Sumner TVA Courier 1.1/1.3 12332 17050S.17 TVA - Sequoyah Nuclear Plant - 101 TN 05-05-17 1230 J. Sumner TVA Courier 0.9 12332 170505.18 TVA - Sequoyah Nuclear Plant - Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12

I l Page I of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeplzales promelas Client: Tennessee Vallev Autboritv Facility: Seguovah Nuclear Plant NPDES #: TN0026450 Project #: Di/rdio11 prepnratio11 illformntio11: Dilution prep (%}

10. 7 2 1.4 42.8 Effiucnt volume (mL) 267.5

i35 1070 Diluer,1 volume (mL) 2232.5 196; 1430 To1al volume (mL) 2500 2500 2500 Test organism illformatioll:

Organism source: In-house culture Age: < 24-hours old Spawn date: o"\\* 1.."\\* \\""\\ Hatch dates and times: C~

• C\\* \\\\. \\\\00 OS*~i.*\\""'\\

Transfer vessel infom1ation: pH - \\.~I., 85.6 100 2140 2500 360 0 2500 2500 'lJ o~o'S s.u. Temperature== c.'.\\..'\\ "C Average transfer volume: < 0.25 mL County: Hamilton Outfall: 101 Commellts: Each concentration was UV-treated for 2 minutes to remove pathogenic Interferences. Test ill{ormation: Randomizing template: PM.e. '\\eu..D\\a) Incubator number and \\(.. shelf location: Arte111ia CHM number: CHM9 14 Drying information f or weight determillntiou: Date I Time in oven: l'l!t*CA"l-, M.~n Initial oven temperature: l.i'\\. (. Date I Time out of oven: !f\\C::.... D*l'"l nA-..n Final oven temperature: L..Q -c: Total drying time: ~~*llo\\l/1.~ Daily feeding mu/ renewal iuformntiou: Dny Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0 05-02-17 01..10 11 \\"1.. :io w 0~:!>1. ~ l\\ ri<.t\\ \\ * (C) '~ C>~o \\. o-\\ o"\\< 'llo* n" I 05-03-1 7 0 "- l:>O I \\ 1.0C w o~c.tb V\\ \\\\O$()l. ~ \\\\llS0\\ -0'\\ O~* l.l.*11~ 2 ".r 05 17 0'-0~ n.. o~ ~ o&SO h l"'\\O~~. ~S no.S~.~lo o"-*i.s.*,,6 3 05-05-17 O\\o\\'I;) 1t{ \\'1.lt:) l.I oSS"L ({' ,,os~.~s rt o.s o ~. :a.i.. t)~*lb*&'li) 4 05-06-1 7 01\\~ \\-1 \\~\\<:. \\,{ ~o ~ I"'\\ OS.OS. \\\\ \\\\O~. \\R QS.*01.*\\1 5 05-07-1 7 <JI'-\\() }{ '~" \\) M OC\\S"L. o~ \\"'\\ l"IS0 S'

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,,osa;. \\~ ~ *(1\\..*\\1 6 05-08-17 <::l'-\\~ .. l \\ \\1.\\.~ ~ OS'?)?) w nn~~. \\1 no~.t8 nc;.*(ft.* 1, 7 u , V\\ >.\\ 05-09-17 04S-'\\ Co11trol i11formatio11: Vl* C..c~oL Acceptance criteria Summmy of test endpoints: % Mortality:

01.

S20% 7-day LCso > 1001. Average weight per initial larvae: o.<-,~~ NOEC 1007. Average weight per surviving larvae: o.t..'\\e ~ 0.25mg/larvac LOEC ~ lO() 7. ChV "'> lN\\1. IC15 > 1001. Page 26of94 SOP AT20 - Exhibit A T20.3, revision 11-01 -1 4

Page 2 of 7 Species: Pimepltales promelas Client: TVA I Sequoyah Nuclear Plant. Outfall 101, UV-treated Date: 05-02-17 Survival and Growth Data Day CONTROL 10.7% 21.4% A B c D E F G H I J K L 0 10 10 IO 10 10 10 JO 10 10 JO 10 IO ] IO IC> IO J {) l~ I() IC l t> to IQ co tO 2 /\\) to IO 10 10 lD IQ lO lD I CJ 10 f() 3 10 10 io 'D 10 lQ l () IQ '0 I() lO t() 4 tD ID 'D 'D ID IQ ft) ( (J I t) '0 l () '~ 5 llJ 10 ID !() (() I() I\\:) ID ID

11) l(J co 6

ID ID ID It) 11;:} It> 'a l(J IQ I C 10 10 7 It> I l) l () I 0 I() I 0 /\\) ID IO /0 t lJ IQ A - Pnn we igh! ~ ~ I"'.~ \\S.o3 \\s.ec /to.~ (5.ot l~O!J lY.~ 13.~~ l5.'4S-Troy color code:: >> L 0!0.XJrC.. llP. 03 l "1.1... Annlyst: iJ..., Dnlc: O"'*cii* 1:r l'S.~ ll = Pan + Larvae '[>'.;ht (mg) Analyst: J.1.00 AJ~ a.135 ~.93 Date: 01<5.) I.1): ~3.Sb ~3. 0-=l >>i~,o:l. ;o.q l ~'-*31.P ;1,l.4$ ~o.SlJ ;l3, I(,. C = Larvae weight (mg)= B - A (.. ~'L 11. t. ~ "'\\. ~l 1.<f:!> ~. b~,.o'-l "\\.O\\ '-*,., "'\\.oc,.o\\ lo. ~C\\,,,\\ Hnnd cnlculnlcd. ~ Arrn lyst: Weight per initial number oflarvnc {mg) = C / Initial number of lar-vne ,.../ .).. ~ t:- O" ~ ~ 'V' "() Hand cnlculated. ~~ ~* "° C>' Analyst: Q. ~* o* o* o* o* o* <:) o* er Average weight per Percent reduction 0-"'°" &- o.\\o'\\lo o.~1. O.""\\"l."b ~.b 1. initiul numbcr of rrom control (%) larvae (mg) Comment codes: c = clear, d =dead, fg =fungus, k = killed, m = missing, sk =sick, sm = unusually small, lg = unusually large, d&r = decanted and returned. w = wounded_ Comments: Page 27 of94 SOP AT20 - Exhibit AT20.:;, revision 11-01-14

l l J J

r;::::t c:-;::l

~ i "-- ~ (' ).... --i-..~~ Species: Pimeplwles promelas Page 3 of7 Client: TVA I Sequoyah Nuclear Plant. Outfall 101. UV-treated Date: 05-02-17 Survival and Growt!t Data Day 42.8% 85.6% 100% M N 0 p Q R s T u v w x 0 10 10 10 10 10 10 10 10 10 10 JO 10 1 /0 10 IC 10 ID co lQ l {) tO 10 10 IQ 2 fO IQ to t \\) L {) l \\) ta 10 { {) I\\) '0 IC) 3 I 0 ID 10 lO lO 10 I() lb lb ID 10 co 4 I 0 l () ID /"{) [ 1) lD 10 LO 10 tO lb ro 5 '{) I (J 10 l \\} I iJ ID 10 ID 10 10 IQ 10 6 I 0 10 IQ I () /Q 10 I~ ID l'\\j ID 10 ID 7 10 ID It:> 10 /{) lb CD 10 I() /0 tO

• D A,. Pan weight (m£t Trny color code:: yciqoo*.s..e-

\\S~<

~!.~ Date:

06. \\\\. f.}--

C = Larvae weigh t ( m g) ~ 13 -A ... ~1 (o.~1. t,,,\\o1. ~.i~ ~.~,, \\t:\\ 1..s1 c.., lolo 1oA'-\\ "'.'-\\'\\ ~."2.1 1.;.~1 Hnntl cnlcnlntetl. ti Analyst: W eight per initial number of la rl'ae (mg) = C / lnitia l number of lan *ae ~ .)-. '-:1"' 'I>

t' ~ ~ "'

tf, tV

q.

~ °' '° IO Hant! cnlculatctl. ~ ~ -.g ~ -.&) "° ~ 'I> \\0 ~ <). ""11 "\\O Cf r;;;;,' ~

• c' Annlyst:

o* Q' 0 o* '\\). o* i:5 Average weight per Percent retluclio11

0. IJ/'t

'-\\. \\ 1. O* '4:-\\.~ 1..\\.\\1. o.~:,'1 s.. '-\\ 7. initial number of from control (%) la rvnc (rn ~) Comment codes: c - clear, d - dead, fg = fungus, k = kil1cd, m - missing, sk - sick, sm = unusually small, Jg= unusually large, d&r = decanted and returned. w = wounded. Co111me11ts: Page 28of94 SOP A T20 - Exhibit A T20.3. revision 11-01-14 J Page 4 of7 Species: Pimeplrales pro me/as Client: TVA I Seguovah Nuclear Plant, Outfall 101. UV-treated Date: 05-02-17 Survival mu/ Growth Data Day 100% Intake Control - Non-treated y z AA BB cc DD EE FF CG HH 0 IO JO 10 IO 10 10 10 10 I (0 10 I 0 10 IQ to 10 ( () 2 to I \\:J to co to to ID 10 3 ID f O I\\) 10 10 10 I CJ IQ 4 lD ID I D ID l b /(j IQ I Q 5 l'O I {) ID 10 I Q I Q I (J to 6 IQ I D I I) 10 f(J J () tO IQ 7 <1'-t 1$Jo\\ 10 "'.,c. I t:> 10 JO {() f\\) A= J>nn weight (mg) llti.16 Tray color code:: i.j~ v cXU lSA4i l'-4. 143 }5,Lt4 lS.e>o \\5.82. \\S.0'.P 15:-:k) Analyst: ,.!{., Date: 01.l.aJa!.

• I ~

3 = Pan + Larvnc weight (mg) ~.\\.l lj /H>.IP8 a1.43 AA~J ~ :;l.12 '"3.lo5 ~.Iv ~. '3'l Analyst: ~.II*(.}- Date: C = Larvnc weight (mg) = B - A (,.So t..os ~.'\\'- (o, i& c,. ~"L "l. t.1J ~.'!,0.... Ir;.'\\ Hnnd calculated. ~ Analyst: Weight per initial number of lan*ac (mg) = C /Initial number of larv:u: '11,.,,'V' a."'IJ tt-. c,,O 6-' ~ ~~ ....,o v Hn nd calculntcd. ~ £.; ""° "° '° o* Analyst: '\\:)' o* o* o* o* \\:) o* Average weight per Percent reduction ~

0. (., 1t:\\

~Ot"" initial number of from control (%) &\\. C)1. ~t>PL~l!Le lnrvne (mg) 0

• to~S Comment codes: c = clear, d
• dead, fg = fungus, k = killed, m - missing, sk = sick, sm = unusually small, lg= unusually large, d&r =decanted and returned, w =wounded.

Co111111e11ts: a JJ Page 29 of94 SOP A T20 - Exhibit A T20.3, revision 11-01-14 Envlronmllntal Testing Solutions, Inc. (\\*n<('ftl r.14~ cV.1 Ac:pl"~'< Cunlrul. No1Mf't'tUt'tl

Coru1ol, UV* lf"U IC'd IU.7%

Zl..-1% 4?.S% 35.6'Y. toO"/* tOU ~l. lntuk.C' ill!!!il!!.l!ll l>unnr-11* ~ ' ISO' l11r-1 ,.,1 0 : l11t?kr: l>unn£ll 1!\\ MSU \\"i!IUC': 1*1\\l U: cc 1111 ££ ff u c I) ~- f c II I J K I. M N 0 r 0 II s,. u \\I w )( z Ail uu 1nhbt 11* "*bt.t*r t1n1I IUtmhrr t1f bn llC lann 10 IQ 10 IU 10 v 10 10 10 10 10 10 10 10 10 10 IU 10 10 10 10 10 10 10 10 10 IQ IQ 10 10 IQ 10 10 10 10 10 IQ 10 10 10 10 10 IQ 10 10 10 10 10 IO IQ 10 10 10 10 IQ 10 10 10 IQ 10 IO 10 10 0.0511 7.J 0.0605 8.7 ". 1'1ri.. irt;::llt (n11.t1 ISSO ISS~ 1116 1570 IS l6 16 56 ISOJ ISIO 11.so 160J ISQI 14 14 IS 06 ,.. JJ IJ6S IS4S ll 76 IS J4 lb 21:i 1603 16 ?b IS 74 l.t.K'J 16 21 IH6 IS 17 14 ** 1'61 IS 11'1 14 6J IH7 1605 MD PMSD TVA I equoya h Nuclear Plant, Outfall 101 Ma 02-09 201 7 Pimephflles pro111e/11s hronic Whole Ef'nuc111 Toxicity Tc *1 El' -82 1-R-02-0IJ. i\\lclhotl IUOU.O Qn:ililv *0111rol Verification of Data Entry, Calculution, 1111<1 ' 1:1tislicnl Annlyscs N0t for Comph;inci: 1\\1..~ nK*m. lim:m~ l.abonnory QC 8

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• fic;in1 DtO"t:rl!nt.:e Pe1ccn1 Mu*unum Sf];01ftar1t 01ff.:ro;.'ll(L' 12Jll

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Otf'llro:ll (~~I lan lM'im-~I 97.~ 0.679 10.6 Not "r1,tic:tblc 100.U Q.69S 6S N'o1 up1*litublt> 100.0 u.696 I.~ OJ 100.0 U.71J 5.11 .J.o 100,0 0.669 .l.I 4.1 1011.0 0.660 ~ *.a 4.1 100.11 0.6J? I.~ SA 97.S o,u s 6.7 9.0 PMSO 11.i 111~aS\\Jr-c 0(1~1 p111.--cmon r~ PMSO is the r11101111u1111,..,'fccn1 dtlf.:u."tK.: bc11.1,~.. '11 the conuol ruld u~a1m.c111 d1J1 can~ <k-cla1\\.*d 11:i11~ucally s:ti;tufrc,ll\\1m11 \\"-hok d Ulh!l'l1 IOloC1ty resc l.o\\ver l>MSD bolWd dcli *.,mmcd b) USEPA (10th t>cre.. "nul~) t 211.. llpp..'f rMSO bound dc1crn11ncd b

• USEl'A c90th pcrc.1111lcl Jm.

l.oWl!r 3.0d *11p.:r r~ I D bollnds,\\'\\..h! dl.'.1.:11ntocJ from th.: 10ih.l 1d U<kh Ptr.:.:l\\f\\k-. MtlCCll\\"clv. or P~I SO d;ma frtJm El'1\\°> \\YET lttt£1l:1hor:iaory V:vt.ihlln\\* S111dy (tJSPl1A 1001:\\ t 11\\f: p, >OO lt.) TVA / equoyah Nuclear Plant, Outfall 101 Ma 02-09, 2017 Start Date: 51212017 End Dale: 5/9/2017 Sample Date: May 2017 Comment.s: UV-Treated Conc.. 0/o 1 2 Non-Conlrol 0.6320 0.7830 UV-Control 0.6320 0.7230 10 7 0.7000 0 7040 21.4 0.7300 0 7010 42.8 0 6870 0.6420 85.6 0.6330 0.7193 100 0 6440 0.6490 lnlal<e 06500 0.6050 Conc 6 */o Moan N*Moan Non-Control 0.6785 0.0726 UV-Control 0.6975 1.0000 10.7 0.6955 0 9971 21.4 0 7228 1.0362 42.8 0.6688 o 9588 85.6 0.6688 o 9589 -100 0.6393 0.9165 Intake 0.8348 o 9 oo Auxili~~ Tests Statistical Analy e Larval Fish Growth and Survival Tost-7 Day Growth Test ID: PpFRCR Samole ID: TVA I SON Outfall 101 Lab ID: ETS-Envir. Tesling Sol. Sample Type OMR-Discharge Monitonng Report Protocol: FWCHR*EPA-821-R-02-013 Test Species: PP-Pimephales promelas 3 0.6300 0.7320 0.7010 0.6890 0.6620 0.6570 0.6270 0.5960 Mo.an 0.6785 0.6975 0.6955 o 7228 0.6688 0.6668 0.6393 0.6348 4 0.6690 0.7030 0.6770 0.7710 0.6840 0.6660 0.6370 0.6880 Willie hypotnesfs test results indicate a slgnlflcant dlllerence n growth for the 100% OuUall 101 sample, tho PMSO was below the lower bound (12%) os1ablishod by EPA. Guidance In EPA 833-R-00.003 (June 2000) Soclfon 6.4. 2 for determining lhe NOEC was followed, and ii was concluded that lhe hypothesis lest yielded a Type I Error. T ransform : Untr;af1Sformed 1-Tallod Isotonic Min Max CV°!. N I.St.at Crl tlcal MSC M ean N*Moan 0.6300 0.7830 10.602 4 0.6320 0.7320 6.497 4 0.7053 1.0000 0.6770 0.7040 1 790 4 0.094 2.410 0.0511 0.7053 1.0000 o 6690 0.7710 5 046 4 -1 190 2.410 0.0511 0.7053 1.0000 0.6420 0.6870 3 145 4 1 355 2.410 00511 0.6666 0 9483 0.6330 0.7193 5 445 4 1 352 2.410 0.0511 0.6688 0 9483 0.6270 0.6490 1.492 4 2.746 2.410 00511 0.6393 0 906* 05960 0.6880 6.718 4 St:>tlstic Critie~I Skow Kurt Shapiro-Wolk"s Test indicates normal dislritlulion (p > 0.01) 0.97487 0.884 -0.2395 0.86461 Bartlett's Test ondocates equal v riances (p z 0 14) 8.38396 5.0863 The control means are not s19n10car'\\UV ditleren1 <!! = 0.67) 0.<:<:695 2.44691 H~pothuls Test !1*1.all, 0.05! NOEC LOEC ChV TU MS Du MS Op MSB MSE F-Prob df DunneU's Test 85.6

• 100 92.5203 1 16822 0.05112 O.C*7329 0.00341 0.0009 o 01612

5. 18 Treatments vs UV*Conlrot Lln &~r 1n1orpo1auo111200 R&sal!'lp 10~)

Point so S6% CL(E.>:E) Skew IC05 42 096 24.500 17.808 122-928 0.4432 IC10 >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 IC40 >100 0.8 tCSO >100 07 .. 06 c: 0..5 0 Q, 04 0: 03 0'2 0.1 0 0 -0 1 0 50 100 150 Dose %~ Ooso-Responso Plot 09 o.e 0.7 ... 06 'i

• * * * *
• __. __. _. _. _____ -_:-.§::-_ ::-. ::-. 7. 7

- ~-"'---i:i--~~ Hall. 0.05 level of significance e o.s

- 0..

0 ,_ 0.3 0.2 0.1 0 0 c 0 u.: 0 z g co 0 ~ ~ 0 c 0 s 0 > Page 31of94 TVA I equoyah Nuclear Plant Outfall 101 - Intake May 02-09, 2017 Envtro nm e "~' Tcs11n9 SoluUon1. Inc. Start Date: 5/2/2017 End Date: 5/9/2017 Sample Date: May 2017 Comments: UV-Treated Cone-% 1 2 Non-Control 0.5320

0. 7830 UV-Control 0.5320 0 7230 10.7 0.7000 0.7040 21.4 0.7300 0.7010 42.8 0.6870 0.6420 85.6 0.6330 0.7190 100 0.6440 0.6490 Intake 0.6500 0.6050 Cone-%

Mean N-Mean Non-Control 0.6785 0.9728 UV-Control 0.6975 1.0000 10.7 0.6955 0.9971 21.4 0.7228 1.0362 42.8 0.6688 0.9588 85.6 0.6668 0.9588 100 0.6393 0.9165

• intake 0.6348 0.9100 A uxiliary Tests Statistic:il Ana lyses Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpFRCR Sample ID:

TVA I SQN Outfall 101 - Intake Lab ID: ETS-E,vir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas 3 0.6300 0.7320 0.7010 0.6890 0.6620 0.6570 0.6270 0.5960 Mean 0.6785 0.6975 0.6955 0.7228 0.6688 0.6688 0.6393 0.6348 4 0.6690 0.7030 0.6770 0.77 10 0_6840 0.6660 0.6370 0.6880 While hypothesis test results indicate a significant difference in growth for the intake sample, the PMSO was below the lower bound (12%} established by EPA. Guidance in EPA 833-R-00-003 (June 2000) Section 6.4.2 for determining the NOEC was followed, and it was concluded that the hypothesis test yielded a Type I Error. Transform: Untransformed 1-Tailed Min Max CV% N t-Stat Critical MSD 0.6300 0.7830 10.602 4 0.6320 0.7320 6.497 4 0.6770 0.7040 1.790 4 0.6890 0.7710 5 048 4 0.6420 0.6870 3.145 4 0.6330 0.7190 5.425 4 0.6270 0.6490 1.492 4 0.5960 0.6880 6.718 4 2.017 1.943 0.0605 Statistic Critical Skew Kurt Shapiro-Wilk's Tes: indicates normal distribution (P > 0.01 ) F-Test indicates equal vanances (p = 0.92) 0.95314 0.749 -0.4316 -0.9929 1.12947 47.4683 The control means are not significantly different (p = 0.67 ) 0.44695 2.44691 Hypothes is Test (1 -tail, 0.05) Homoscedastic t Test indicates significant differences Treatments vs UV-Control 0.2 0.1 0 .l: c: 0 c: 0 z Page 32of94 g c: 0 u > 0 MSDu MSDp MSB MSE F-Prob df 0.06046 0.08668 0.00788 0.00194 0.09029 1, 6 Dose-Response Plot 0 0 ), __ ....,...._~ Page 5 of7 Specie : Pimepftnles promelas Date: 05-02-17 Client: TVA I Scguovah Nuclear Plant, Outfall 101. UV-treated D *1 Ch at JI emtstry: Day (Analyst identified for each day, pcrfom1cd pH, D.O. and conductivity measurements only.) 0 I 2 Analyst y.- 1.J.- I>-- "'v Jv Concentration Parameter

• ~ -
• ~

~.,., -- f ~ -::: ~.::

• .....c..o.:..

_ ~s. ~ oH (S.U.)

a. 1'\\

'l._q~ (b{}l.a ':\\..u" e.ov "".},"""' DO (m!!.11.) Ila t;\\-.f.O '+A (O.~ '~.a ~-2 Conductivity ~

• ~

322- .3Z6 01C\\ 1........ __

• t '~ ' 1 CONTROL

(µmhos/cm) I ~,....

• Alkalinity i ':'

  \\' "

\\p...\\ .. ~ ~

• . '"'-...-N'"'

UV-treated (mg CaCO;/L) ~\\ . *~*~.: 'r.:.

• Hardness

..,~*.Ir- ~.. - ~ * --..,. &~ &l.o (me CaC03'L) -=-*

• Temperature ("CJ "t~-"\\

\\.'-l * \\o \\..'\\*lo ~\\.-~ "1:-\\..J ~**\\."\\.. pH(S.U.) c&.\\6 ll.f.>'t e.4"\\ ')._\\JI\\ eo~ ~.~ DO (mwL) 1\\-.C\\ ~.v ~-q (O.~ ~.q '+.l 10.7% Conductivity ~OC\\ 3l0 3oto fomhos/cm) "Temperature ("CJ '1.'\\.& 1.~A '"\\.'-\\*l '"\\..'-l-1 "\\.'-\\. '\\ "'\\A.A pH (S.U.) e.1v t:\\-.0~ ra-' o '.\\.\\tl A1~ ~.5"}- DO (mwL} ':\\.~ ~-"' 'l.q \\O.'l ~-~ {J).v 21.4% Conductivity ~ 2>12. I> (J.lmhos/cm} ut\\ . ~;. "'Temperature (°C} "\\.'\\. << ~-1 \\."\\* 1 \\1-\\.\\ LS:o '"'-"'* '{ nH(S.U.J S.14 ~.)')- ~.\\O '+.\\O\\ ece ~ ..... ~ DO (m!?/Ll ~'1 I}.:::; '+q (/1 \\ 0.0 in< 42.8% Conduclivity "" 1-2e:::J.o I (~1mhos/cm) UPO "'Temperature C°C) i.'\\..g \\..'{.'\\ .,_.. t. ~ ~\\.S l..~*O ,.i{.I.. pH (S.U.) .~\\~ '\\..t>S e>.\\ \\ '.\\.~ ao::\\ ~.~ DO(m!?/Ll ~o 'l-.5 'tY lll'Z. 0.0 l4."1-85.6 Vo Conductiviry l~ ,q' 16<.o (~1mhos/cm}

• Temperature (°C) 1-"\\.~

t.*V1 "'\\!-\\. '\\ 1.."\\-1 '1.'S. 0 ,_~.c.. pH (S.U.} 0.\\$" /}.PM e>.~s "+,;f 0.0'-l "\\..5tP DO (mg/L) {Jj.J) ~.<I\\" t:\\.&I u,?_ 6.o <J1.eJ Conducti\\'ily I l,/J::)

• ~s l~O (umhos/cm)

.c

• Alkalinity j

-,'f~'~ 100% S(\\ toS (mg CaC03'L) ~

• H:trdncss t,...\\
• -7~

~ --. ]ij ;~~ vl.o (mg CaC03'L)

• • :ii. *.
• TR chlorine (mg/L)

< 0.10 r..-:-* -<o.10,,

• Temperature (°C)

"\\..~. 0 l."\\ * ~ 1.'1. '\\ "L"\\. cf "\\."i. \\ '"l.'{. '° pH (S.U.) 6.11-' r1dl'< Pln~ ll1Al A03 'l."53 DO (mg/L) ~-1 ,.J..l.P ~./J l.l)."'\\ A.O !.#,~ Conductivity ~ tll3 t 146"'

!~

168 ,'fl; (umhos/cm) . ~:t r .\\, .i...:;. 100%

• Alkalinity

. -~ . J~ (9\\ - ~r \\c~ (m2 CaCO;/L) lntal<c

• Hardness

~'1

• I I (ma CaCO;/LJ lab

.,_,_. ~ 1&.1'. - ~

• TR chlorine (mg/L)

<o.tO ~- <0.10

• Tempcrnturc (°C)

L.,"(.'i '\\.-(.c\\ 1.~-~ '1."\\. s

n. \\

1,\\.(, 'i Initial Finni Initial Final Lniti:tl Final ,......... mpcraturcs pcrforlllcd at the lime oftest initiation, renc' al or termination by the analysl identified in the Daily Renewal Information table located on Page I. ...... wiR,lk inily, h dness and tot;il residual chlorine perfonned by th analyst id~nti(i~cl on rhc bench ~heel ~pccilic for each anal y~ i ~ and trnn ~crihecl In 1hi~ hcnch ~heel ~__..,__. Total residual chlorine was performed on non-treated Outfall IOI and lnrakc samples. Page 33 of94 SOP AT20 - Exhibit AT20.3, revision 11-01-14 c--:::J lf=l ~ - I Species: Pimeplwles promelas 1en : eauova uc ear an. u a Cl' t TVA I S h N I Pl t 0 t[ JI IOI UV Page 6 of 7 -trcate d D ate: - - 05 02 17 Day (Analyst identified for each day, performed pH, D.0. and conductivity measurements only.) 3 4 5 6 Analyst v-- ~~ - IA- ~ .Iv J.f-..1 Concen-Paramete1* ,~-. I ~ ~ trntion (( i - nH (S.U.) f?>.oS +.~l ~.\\O '.}.§ B f'l,.. ')..A~ 0.\\"' rJ.Sl-DO (ml!/Ll g.o 'J.~ c;c.. 2..- "'* -s 'lo~ 'l.< !'.}Pf (jf,z... Conductivity 22.LP i r::t 3\\2-32\\ C111nhos/cm) CONTROL .r -

• Alkalinity

~ .. ~"' ~ '1~..... UY-treated \\cl1- ~A" (mg CaCOJILl ~

• llardncss

~

• ~

& '4 -.~..--- -, I (ma CaCO;/L)

• Temperature (°C)

""\\........ ~ \\..-t*"- "'U\\* ~ '"\\.... \\. '\\ \\..--\\* \\ \\.."'\\. "'\\ ~'-\\-~ "\\..4,.1-- riH (S.U.) A.i~,, '":f. ~4-g' *\\ 2.- 'l,,o..\\ t'\\ l~ 'l.e'-t 0.\\1.p ~.\\('1 DO(m!!IL) (JL() 1..1- <l. \\ lnVl e.o ~.5' ~.q ((), '2... 10.7% Conductivity ~. 210 ~'ts-3"1 3oS I (jam hos/cm)

• Temoe*rnture (°C) b-\\.\\

'-'-t*S ""\\.'\\.<\\ .......... \\o "'\\.~.'" ~-'"\\ \\.."'-*" "\\.~*"" pH (S.U.) e.o4 -J.-:n- ~.\\\\ ".t*-5\\P ~~ '.l..~3 ~.\\-:\\.

• • • 1.JA DO (mlifl.)

~.o -:J.r-+- <6.1~ to.+ 6.J.J "".},"' Jl..tJ <.P \\ 21.4% Conductivity ~ ~otld c!X~3 ~q+ 2°t?, (um hos/cm) I W'

• Temperature (°C)

-,s...Q "'\\.'l

• c;

~-0 '"\\..'-\\* \\.:. "'l_..\\.~ \\.~*~ ~-0 '"\\.-l*'-' riH (S.U.) ';}.°'S' ".1:*:n- ~.o'l 'l.o..l e.O'\\ 'l.~i.t e.\\e, T.}.,~q DO (m2'Ll 6..0 J..1- ~.\\ I0.6 t!>.O ~.14 "),,q 14.0 42.8% Conductivity a1..1l ~.... - I* {11111hos/cm) ~~'° ~143 ~5(p

• Temperature (°C)

"'\\S. 0 "'I.... la \\S.o -..,'-\\. b -U-\\. ~ "'\\A*S '"\\.Ci..o '-"\\*,_ 1>H (S.U.) '-l-A'l ""J..'1-r ~.\\Q l'.\\-.S"~ OIJ"'IL.4 ".\\-.0(0 0,\\G:\\ '.}...~ DO (mg/L) 0.0 'J.'l-({. ' I0,1..D es.1 ':).ta /.}.q <P.o 85.6% Coruluclivity ~ ll\\) \\Kl l'13 l q l (µmhos/cm) ~ ~ Temperature C°C) l.S.. \\ "\\_ '-t

• le, u.a L."\\.1 l.'\\.'\\

L.!.\\*l-. ~.o ""\\..'-\\* "L pH (S.U.) f:')O?i. ?."'=Iv "iLD 't ~-SV 01'11.11 '+.A".} e.\\a .fl.:*I~ DO (mlifl.) e.o l.t- ~. l (a.~ e.o ':)~ 9-0 ~q Condllctivity \\ lt>'":r 11.tS-l~ ll.o~ (µmhos/crn) -*.-i..;. ~

• AJknlinity

' r -~ 100% ""°' ' ~:l (mg CaCO;/Ll

• ll:irdncss

. ~ (mg CaCO;/L) (.~

• ~.'

~ ~ ~

• TR chlorinr (mg/L)

~* -. I f"l.I(') - ~ .4}-...

• Temperature (°C)

'"\\S. \\ "\\!-\\. s -u;. \\ -r-\\*.S L.S.O "'t.L\\*1- \\.~. \\ '1..-t.~ DH (S.U.) ~DS -'J,,f'C( ~.'i::l"1-r~.lJ2. 0.0""} .tlC\\\\ {:).la '}.~0 DO (ml!/Ll so -i...v 'J..CJ f.o.~ '6.o "l'l- -:JA ':).Cf Conductivity 1 ~ \\~1-(1m1hcs/cm) iuie l '4 \\ 11v -'*~ ~-* 100% "Alk:ilinity <o\\ Intake (me CnCO;/L) J

-;i, '

_li 'Ji. ~-...-

• Hardness f'

" - ~ 10 I* I~:.. (mg CaCO,;/Ll .,..-:1

~:

i' '31 I* ~

• TR clllorinc (mg/L)

LD*\\0 ~ ~- ./

• Temperature (°C)

"l$..O l..J.-lo 1.S* \\ 1,.'{., ,~:o 1..'-l* ~ 1$. \\ 1..'"\\.S Initial Final lnitial Final Initial Final Initial Final llUft n '** : wi 'tc 1peratt1res pcrfonned at the time of test initiation, renewal or tcm1ina11on by the analysl idcntilied m the Daily Renewal lnfonnation table located on Page I. "-"*v*.. Alka 'nity, hardness and total residual chlorine performed by the analyst identified on lhe bench sheet specific for each analysis and l ra n~ cr i bed to lhis bench sheet tf.J>y: . Total residual chlorine was performed on 11011-trcated Outfall IO I and lnlake samples. P e 34 of 94 SOP AT20 - Exhibit A T20.3, revision 11-0 1-14 ,_ ~ Page 7 of7 Species: Pimephales promclas Client: TVA I Sequoyah Nuclear Plant, O utfall 101, UV-treated Date: 05-02-1 7 D *1 Cl auy 1e1111sfrJ1: Day (Analyst identified ror each day, nerfonncd pH, D.O. and conductivity meas\\lrcmems onlv.) 0 l 2 Analyst if..; ..}--- iJ...,; IA 1-- ,..)-. Concentration Parameter ~

• -~

~ ~.. nH (S.U.) e.2\\ 'J..c:Ji1- ~.IQ '\\.ut4 a.oe, -t.-sz. Control DO (m!!IL)

• +~

'.t.s

• .;.7-r~.2..

+:1- ~. I.cl Non-treated Conductivity (~u11hos/cm) ~2.l "l.'20 ~- 3lC\\ q~-;; ~- I

• Alkalinity

~..r"" "° \\ ~; \\o L- .i (111~ CaCO;/L)

• A,_

~ ~ -

u.

- ~ --

• Hardness

~ 'i 4>& t1- 'j 11112 CaCQ,/Ll .~ -~ ~ "Temperature (°C) ""l."\\.\\ ~'-\\- < "\\.<\\..., ""\\:'\\. s 1.."l.\\ '1..'-l. lo Initial Final Initial II Final Initial II Final Day (Annlyst identified for each day, performed pH. D.O. and conductivity measurements only.) 3 4 5 6 Analyst v--- (,L. I).; ti'- .µ Vv ~ ~ w r 1,:.. Concen-Parameter

• ~

r

• trntion

!~ *. ~** - ~ '..

• .r' 6

u.._

nH (S.U.) 5.\\~

+."i2..

~ ~ °"~ t-$4 e.l-s- 'l.A..! PU°I '.l.4'\\ . ~ Control DO (mg/L) J...~ -:i. 'b

• -~

IO*--\\ "l.-:r 'l-.112 '}.~ G! o Non-treated Conductivity ~. s2v 3 rl 32.4

w

(µ mhos/cm)

• Allrnlinity

~t.V-'""' ~' l~o-"\\ ~- - (mgCaCO:i/L) '°::::,

• Harclncss

~ ~ ~ - 8b (mg CaC01/L) ~

• Temperature (°C)

-,.,\\. b ""\\-'-\\ \\.'-\\.lo ~. "':> ""'"'* \\ &..'-\\. ') "'\\..'-\\. ~ ~ tl.!.'-1 Initial Finni Initial Finni Initial Final Initial Final

• Temperatures pcrfom1ed nt the time oftest initiation. renewal or tenmnalton by the analyst 1dent1fied in the Dally Renewal 1nromiat1on fable located on Page I.

Alkalinity, hardness and total residual chlorine pcrfonned by the onalyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:-*. Page 35 of94 SOP A T20 - Exhibit AT20.3, revision 11-01-14 ~ I Envlronme.ntal T~srlng Solution)., Inc. Concc11tmtiu11 11nrumctrr !ull ISlJl 00 (mi!/Ll

Conlrol, Conduclivity (11mhos/cm)

Non*lrc:ttcd t\\llmlinitv (mgn_ C:iCOJ) ll nrdncss (1111!/L C :1CO,} Tc1n1>er:uure ("CJ 11111<;;111 no (m~n.1 0 11l rol, ConductMtv (11mhos/cml V-trence!I t\\lknlinily (mf!/L C:lCO,) Hardness (ntt:/L CuCO, ) T emuer:llure (°C) nll ("Ill DO(m ~L) 10.7% Conductivil\\' (1rn1hos/c111) Tem11rr:llure ('C) 11111 !SU) DOim~/Ll 2 1.Jo/.. Cond uclivit\\' (µn1hos/c111) Te11111ern1ure ("C) 11>11 (SU) DO(mi!/L) 42.8"/., Conrluccivilv (ua1hos/cml Temoeralurc t"C) 1111 (SUI DO (nll.!.IL) 8~. 6*~. Conduccivicv (11a1hos/cm) Tcnm<rulurc (' Cl nil rSUI DO ( m ~Ll Conduclivil1* (11*1 hos/c111l 100% Alkalinity (mg/I, CnCO,) ll nr1lnes5 (mglL CnCOJ)

• Total n csidunl C hlorine 11111!/L Tcmpcralurc ( Cl lull (SUl DO (m11/t.)

Condur1ivitv lua1hos/rn1\\ 100% lntnk< Alkalinity (mgll. CnCO,) ll:mlricss (mgfL C11CO,)

• Tomi llcsiduol Chlorine (m!!.IL Tcmuerucurc ( Cl TVA I Sequoyah Nuclear Plant, Outfa ll 101, UV-treated Ma 02-09, 2017 On *O lnitiul 8.2 1 7.9 321 6 1 88 24.7

8. 19 7.9 322 61 88 24 7 8 18 7.9 309 248

8. 16 79 29 1 24.8

8. 111 7.9 260 2<1.8

8. 13 8.0 186 1-1.9

8. 15 8.0 160 59 M

<0. 10 25.0

8. 17 8.1 163 6 1 64

<0. 10 24.9 Pimepltale.~ promeills Chronic W hole Effluent Toxicity Te t EPA-82 1-R-02-0 13, Method 1000.0 Daily C hemical Analyses Dul' I Ou\\1 2 Onv 3 D:tv 4 Finul lnillnl Fimtl lnitlnl 17in11I lnitinl Finni lnithtl 7.92 8.10 7.66 8.08

7. 52

8. 13 7.83 8.08 7 s 7.7 7.2 7.7 6.6 7.7 7.8 7.8 318 3 19 326 3 17 62 63 82 86 24.8 14.7 2*1 s 24 7 24.6 24.6 24.'I 24.6 7.93 8.06 7.65 8.06 7.69 s.os 7.8 1 S. 10 7.6 7.9 6.8 79 7.2 8.0 7.7 8.2 325 319 326 3 12 64 62 80 80 24.6 24.6 24.7 24 8 24.2 24 8 24.2 211.s 7.89 8.09 7.6 1 8.07 7 58 8.06 7 74

8. 12 7.6 7.9 6.7 79

7. 1 8.0 77

8. 1 318 306 3 10 295 24.9 24.7 24.7 2*19 24.4

'.!4.9 24 5 24.') 7.87 8.10 7.6 1 8.08 7.57 8.04 7.77

8. 11 7.6 7.9 6.7 7.9 6.6 8.0 7.7 8.2 305 292 296 283 24.7 24.7 24.7 25.0 24.4 25.0 2~. 5 25.0 U7

8. 10 7.61 8.08 7.57 7.98 7.77 8.0'l 7.5 7.9

6. 1 8.0 6.5 8.0 7.7

8. 1 267 256 261 250 24.9 24.8 24.5 25.0 24.0 15.0 24.6 25.U 7.85 8.11 7 59 8 09 7.57 7 99 7.77

8. 10 7.5 79 6 z 8.0 6.7 8.0 7.7 S I 191 186 193 187 24.9 24 9 24 7 25.0 24.6

25. I 24.6 25.U 7.84 8.05 7.51 8 04 7 56 8.03 7.76 8.0-1 7.5 7.9 6.2 8.0 6.8 8.0 77

8. 1 165 160 167 165 65 69 66 66

<O 10 <0. 10 2<18 24.9 24.8

25. 1 24.6

25. 1 24 5

25. 1 7.85 8.05 7.6*1 8,03 7.53 8.05 7 78 8,07 7.6 8.0 6.9 8.0 6.9 8.0 76 7.9 165 158 168 16 1 65 67 66 70

<U. 10 <0. 10 24.9 24.9 '.N.5

25. 1 24 4 25.0 2-1.6

25. 1

• Nole-ro1nl rcsnlunl chlonnc wos performed on non-irenlcd Oulfnll IOI and lmak~ *ampks Finni 7 511 6.4 24 3 7.55 6.5 24.4 7.5*1 66 24.6 7.56 6.7 24.6 7 54 68 24.6 7.58 6.6 247 7.56 6 7 24.5 7.62 66 24 7 Project numllcr" 1?332 Dav s I nit In I

8. 15 7.7 324 24 7 8.06 7.9 317 24.7 8.09 8.0 304 2tl.8 S.08 8.0 297 211 9 8.09 8.0 263 24 9 8. 0~

8 I 193 24.9 8.06 8 0 169 25.U 8.07 8 () 170 25 0 ~~~~~~~- Dnv 6 final Initial Final 7 84

8. 19 7.49 7.6 7.8 6.0 320 24,5 24.S 24.4 7.83

8. 14 7.57 7.5 7.9 6.2 321 24.4 24.8 24.2 7.84

8. 16 7A9 7.5 7.9 6.:!

305 2*1.4 24.9 24.4 7.83 8.17 7.48 7.6 7.9

6. 1

'.!93 24.3 250 24.4 7.84 8 18 7.49 7.6 7.9 6.0 '.!56 !4.5 15.U 14.2 7 86 8 19 7.49 7.6 7.9 6.0 19 1 24.2 25.U 24.2 1.87

8. 19 7.48 7.6 8.0 S.9 166 24.2 25.1 24.6 7 9 1

8. 18 7A8 77 7 9 5.9 167 24.5 2 5 1 24.5 File: sqn101_050217chem.xlsx Entered by: J. Sumner Reviewed by: _

l Page I of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaplmia duhia Client: Tennessee Vallev Authoritv Facility: Seguovah Nuclear Plant NPDES #: TN0026450 Project #: Difutio11 preparation i11formatio11: Dilution prep (%) 10.7 21.4 Efnucnl volume (ml.) 267.5 535 Diluent volume (ml) 2232.5 1965 41.8 1070 1430 Total volume (ml) 2500 2500 I 2500 Test or a11ism source information: Orounism aoe: < 24-hours old 85.6 100 2140 2500 360 0 2500 2500 County: Hamilton Outfall: 101 Comments: Test i11formatio11: Randomizinn tern late color: Date and times organisms were bom OS*d\\.*\\"\\ Ola~'> TO 0'\\()0 lncubntor number and shelf t--b_cl_we_c_n: _____ __,-,.--.,,.--.,.......-'----....-,...---=--..-----t locntion: Culture beard: Daily re11eival information: Day Date Test initiation and feeding, MHSW Snmple numbers used renewal a nd feeding, or batch used Outfall 101 Intake termi nation time 0 05-02-17 O~"l.() o\\o\\*U--t1 ~ l '"\\tfS.11\\. (J"t..,. \\"\\OSO \\. o..\\ I 05-03-17 C>r1..~ o-\\*'\\le*O & \\""\\~O L O"b n.Oi"o\\.O"\\ 2 05-04-17 f'lF.."\\.." o"\\*11-* r\\ ~ L\\OS.~ *~S \\"\\O\\~. ~~ 3 05 17 Od~ o~*t'->*l'1 e, \\1. OS(]T) * ~~ l \\ l>~a\\,. ~I. 4 05-06-17 o& 3 <;. (J ~

• O\\. * \\' nosos. n

\\"\\O$oS. \\ & 5 05-07-17 o~~o O~*II\\.*\\1 \\1OS.OS.\\1 \\"'lOS~. \\& 6 05-08-1 7 l)&1.\\ OS*<n.*\\"\\ \\ '\\Cl~I)~ * \\~ l10CS.cS'. \\i 7 05-09-17 ot'l..\\ oS-o\\*t'\\ Analyst At A-<- j{ }( -}\\ i j1 ~ Co11trol iliformntiou: S ummary of test endpoints: Cootrol-1 Cootrol-2 Acee lance criteria % of Male Adults:

01.

c 7~ S20% 7-day LCso % Adults having 3' Broods: lCf)1. l0(:)1. ~ 60% surviving adults NOEC % Mortality: ~1.

1.

S20% LOEC Mean Offs rino Female: 3.2 ~\\.""' ~ 15.0 offspring/female ChV %CV: c.c..~7. s . ~1. < 42.0% lC2s Page 37 of94 SOP AT! I - Ex11ibit ATI 1.2, revision 11-01-14 l \\ - _,.) t-.dl.iJMJNJ~Llrl(. Page 2 of7 1 Species: Ceriodapl111ia duhin Client: TVA I Sequoyah Nuclear Plant. Ou tfa ll J OI CONTROL-1 S urvival mu/ Reproduction Data Date: 05-02-17 Replicate number Day I 2 3 4 5 6 7 8 9 10 I Young producctl () 0 0 D 0 0 0 0 0 u Adult mortnlity L. '- \\._ \\_ 2 Young producetl ("") ("'} a ("\\ t'I (') c 0 c 0 Atlult morlnlily \\__. '- \\__. \\._ \\.,_ \\..._ 3 Young producctl 0 ("\\ f"'I 0 (') 0 0 0 0 0 At.lull mortality \\.... \\..._ \\.._. \\...- \\..__ L. \\_ '-- \\_ \\..._ \\'ounr; produced ~ \\.., l..\\ s '5 s I..\\ s '5 Ad ult morlnlily L L \\.... \\.,_ L \\._ 5 Young produced l.C n \\Q \\. 'l.... \\ 1.. I~ \\Q I t> \\~ \\ \\ Adult mortality \\._ \\.._ '- \\._ \\.__ \\.._ G Young produced n 0 D 0 0 0 0 0 () () Adult mo1*tnlily L \\_ '- L-L. 7 Young producctl "- \\I.. '~ \\ "?::> ' '5,, '\\e \\., -\\ '(.,, Total young produced ~~ ~~ "1~ ~t> ~1. ~~ ~b ~ -z_ ~~ ~'"L.. Finni Adult i\\lortnlilv ~ X for J'd Urooib ')£.. ")':-. ~ ")C- )<. "Y- ')(:.. 7'--- I< Norr:: Adul1 mort:rhly (l - h\\*t', 0

• dc:3d). SB
• sph1 brood (ttr.gle-brood splll bcm'ttn 1wo d~)'S). CO
• cnny O\\'Cr (orfspnns ~ m c:d O\\'tr with ;1duh dun~ rnn.sfcr)

Co11cei1tmtio11: % Mortality: I

01.

Mean Offspring/Female: I 3l.'\\.... CONC: 10.7% Survival and Reproductio11 Data Rcplicnlc number Day I 2 3 5 G 7 8 9 10 I Young produced 0 0 0 D 0 0 0 (') 0 0 Adult morlAlity L \\._ * \\..._ \\_ L L 2 You ng protlucctl I"\\ ("', (\\ o* n 0 0 t'i tJ 0 Adult morrnlity \\..__ L \\..._ L L L J Young prod uced (\\ 0 0 0 0 () 0 0 0 u Atlult mnrlnlity L L L L-L L \\.__ L L .j Young produced s s ~ ~ '..\\ 4 '{ s 5 Adult mortnlity L. L L L L-L L L L L 5 Young produced \\ l.. "' \\ 1... I\\ ~ "'!> \\ '-'\\ \\ '- \\\\ n.. Adult mortality \\.._. '- L \\.__ L L-L G Young producctl 0 I) /) r., {j 0 0 r., 0 () Ad ult mortnllty \\_.. '-- \\.- L.. \\..__, 7 Young protlucetl \\i l &' I'"\\. \\e \\I I~ \\ \\o \\ l$ I I..\\. Total young producctl ~'-\\

!> k.

3\\

!> 1..\\

~~ 3\\ ~ J ~s ~\\ ~\\ final Atlulr Mortality L-. L.. L.. ~ '- .Vot~: Adult nte)tl31it (L.. lrt-c. D ::s dcl d), sn. sphl brood (1msfc brood spl.11 bc1\\\\CCR l\\\\'O dJys). CO - c.lrrv O\\'Cf (o sonnc. a.me O\\'Cr \\\\1l.l u t umi: lDM er. h d I d r.) Co11ce11tratio11: %Mortality:

07.

Mean Offspring/Female: 3L.f % Reduction from Control-I : -s. \\?. Page 38of94 SOP ATI I - Exhibit ATI 1.2, revision 11 14 Page 3 of 7 Species: Ceriodap/111/(( tlubi(( Client: TV A I Sequoyah Nuclear Plant, Outfall 101 Date: 05-02-17 CONC: 21.4°/0 Survival and Reproduction Data Rcplicntc number Day I J 4 5 G 7 8 9 10 I Young produced 0 {) n 0 0 n 0i 0 0 0 Adult mortnlity \\._ L... \\..._ 2 Young produced ("'} 0 n 0 {) CJ 0 0 n Adult mortality \\..... L \\_. \\..... J Young produced ('} n 0 0 0 0 0 0 0 0 Adult mortality L \\.... L \\.._ L 4 Young produced '5 '-\\ '-\\ '"-\\. s '--\\ '"'\\ "'\\ l...o Adult mortality \\.._ '- \\..... 5 Young produced \\~ \\ !t \\,_ \\~ \\C \\::, \\ "1.. \\() \\\\ °' Adult mortality \\_. L \\.._ 6 Young produced 0 0 D 0 0 0 0 0 ri 0 Adult mortality L L.. L. \\.._ 7 Young produced \\ °' \\., \\ -s \\ \\.o \\ C( \\ \\c, ,~ \\ ~ '"\\,, Tomi young produced !>-"'\\ ~'° ~\\ ~::, ~1. ~'-\\ b\\ ~~ "b,_

.\\

Finni Adult Mortality \\._ \\..__ \\..... \\..... \\..... \\._ No1.. *: 1\\dul1 mort;,hty (L

• ll\\'C, O
• dt:ad). SO
• ipht brood (s111sfc brood split bc1wccn l\\\\'O dJ )'S), CO
• a rrv O\\*c.~ (olfJonnc C'.lmc:d over 11h 3dult dunnu tninsft"r)

Co11ce11rr111iu11: % Mortality:

01.

Mean Offspring/Female: ~~.lo % Reduction from Control-I: --i:1't CONC: 42.8% Survival mu! Reproduction Data Rcnlicntc number Day I 2 J 4 5 6 7 8 9 JO I Young produced 0 0 0 n () 0 (') 0 (') () Adult mortality L L L L L L L L L 2 Young produced 0 ("'\\ f""I 0 t\\ ~ 0 a 0 Cl Adult morrnlity \\._ \\....._ L \\._ \\....._ \\_ \\_ \\..__ \\,.._ '- 3 Young produced 0 0 t"\\ 0 0 0 0 n 0 () Adult mort:ilily L-L L L L L L L \\.._ 4 Young produced ~ 5 s s "'\\ ~ s l:... c_ Adult morrnlity L. '- \\..... ~ 5 Young produced ' ""l.. ~~ \\ "'l. \\ ""l.. \\ '-\\ \\ t> -\\ \\ '?:> \\ "l.. \\~ Adult mortality L \\_ L L \\.......- \\..... \\..._. 6 Young produced 0 0 i\\ 0 0 ~ 0 a ('\\ 0 Adult mortnlity \\_ \\..- L \\.._ \\....... L L L 1.... L. 7 Young produced l '\\ \\8 'l...C \\S \\$ 't:\\ '\\c. \\~ \\ '\\ \\ -s Total young produced

,~

~l. o\\ ~.,_ 'OC> ~~ ~s ~ '"'\\ ~l.o ~~ Finni Adull i\\lortnlity L \\...... Nau : 1\\duh moms1i1y (L

• fh*c. D
• drJd), SB
• spG1 brood (.s1n_.;lc brood 5plu bc1wc-cn 1wo dl)'i}, CO
• curv O\\'~ (offspnni:: comrd over wnb adull dunn12. Lr:1rufcr)

Co11ce11trntio11: % Mor1ali1y:

01.

Mean Offspring/Female: 3~.'t % Reduction from Control-I: - ft>.~7. Page 39 of94 SOP ATI I - Exhibit AT ! 1.2, revision 11-01-14 \\ ----- _,, c...1n.NN1'Ul rutlnqklv:l.:l* La...:.. Page 4 of 7 Species: Ceriodap!tuia tlubitt Client: TVA I Scquovah Nuclear Plant, Outfall 101 Date: 05-02-17 CONC: 85.6% S urvival and Reproduction Data l{enlicatc number Day I 2 3 4 5 6 7 8 9 10 I Young produced [) 0 CJ 0 0 (\\ 0 l°'I a 0 Ad ult mortality L L L \\_ 2 Young produced 0 () A {) (\\ 0 (') 0 0 n Adult mortality \\ L \\_ \\._ \\_ '- 3 Young produced fl 0 0 0 0 0 a 0 0 0 Adult mortality L- \\_ '- L L \\..... \\.. Young produced 5 '° '-\\ l.. 'S ~ \\..\\. Adult mortality \\_ \\_ \\_ \\....... 5 Young produced \\~ \\ '"L \\C..\\ \\~ \\\\ \\~ \\~ \\ '°';:) \\~ l\\ Adult mortality L \\...... L \\__. \\....... \\._ 6 Young produced 0 0 () 0 0 0 0 c 0 0 Adult mortality L L L. L-L- \\..... \\,_, L.. 7 Young produced \\ &. \\~ \\~ \\ '\\ l\\ \\~ 1..0 \\.i. 1..o l\\ Total young produced !:>"""\\ ~~ ~--\\ ~~ ~1... ~~ ~i ~lo ~'\\ ~1..:.. Final Adult J\\'iortalil)* Not(: Aduh monihty (L

• lwc, 0
• dead). SB =- splll broocJ {sm.£,lc bfoOd spin btl\\... -cen cwo d~ys ), CO s cu h d I rv O\\'cr (offsonntt crned C\\'ct wu a u l dunnl! trans er).

Co11ce11tration: % Mortality: n1 Mcon OfTspring/Female: ~.'\\ % Reduction from Control-I : -'\\S. \\"l. CONC: 100% S urvival a11d Reproductio11 Data Rcolicntc number Day I 2 3 4 5 6 7 8 9 10 I Young produced a ('> (\\ n 0 () (') ('., (\\ 0 Adult mortality L L L L L \\_ L.. 2 Young produced () 0 0 0 0 fl (\\ 0 (""\\ (1 Adult mortality

• \\......

\\..._ \\...._ L \\..... 3 Young produced n 0 0 0 0 0 0 0 0 0 Adult mortality L. L-L L L L L L.. L L -I Young [Jroduccd '-\\. s .s

l.

s ~ ~ s Co '-\\. Ad ult mortnlity ~ '-- L L \\... \\._... 5 Yo ung produced \\~ l'-\\ '... l \\,_ \\'-\\ \\"b -\\ \\\\ '""L J 1-Adult mortnlil)* L.- 6 Young produced 0 a () 0 () 0 () 0 a ('"'\\ Adult mortality \\__.. L ~ L l-- L \\.. L.. L \\...... 7 Young produced i..\\ \\""\\ \\i '2 \\~ 10 L "\\ l '\\ 1...0.., Total youag proiluced ~& ~le ~.., ~'- 31 3'\\ 3'\\ ~s ~~ ~~ Final Adult Mortnlity \\._.. \\...__. \\,_ \\.._ NOii*: Adult monallly (L 11 lwc, D. d~d), SD.... split brood {tin!'JC brood spltr bc1wccn lWO d.iys:). co - Q n O\\'Cf {offs'2rirut amcd ovt'.r with ~duh durinc. lransfcrl Co11ce111ratio11: % Mortality:

61.

Mean Offspring/Female: 3'o.~ % Reduction from Control-I: - \\\\.'\\ T. SOP A Tl I - Exhibit AT I 1.2, revision 11-01-14 Page 40 of94 l Page 5 of? Species: Ceriodnpl111ia dubia Client; TVA I Sequoyah Nuclear Plant, Outfall 101 Oa te: 05-02-17 CONTROL-2 Survival and Reproduction Data Replicate number Day I 2 3 4 5 6 7 B 9 10 I Young produced (") 0 0 () () n 0 0 0 0 Adult mortnlity L L L. 2 Young produced 0 0 0 0 0 () a () 0 n Adult mortality ~ '- \\.._ '- 3 Young produced 0 0 D (J (', 0 (') 0 c n Adult mortality L \\..._ L-L- L_ L L... .j \\'oung prod uced w.. "-{ "-\\ "' ~ "'-\\ "-:\\ '-\\ s "-\\ Adull mortnlily \\._ '- L ~ '-- \\,_ 5 Young pr.oduccd l '- \\ "'\\... \\~ \\~ \\\\ \\0 \\0 \\0 \\\\ Adult mortnlity \\._ \\..._ \\.... G Young produced 0 () 0 0 ('\\ (j C) 0 () () Adult mort:tlity \\,_ \\.._ L \\..... \\..._... '- L ~ L 7 Young produced \\""\\ \\S \\S \\~ \\~ \\~ 1'-1, \\& \\~ I \\o Total young produced ~~ :-,\\ "!> '"l.. ~"1. "3~ ~\\ "l.~ ':!> "2. ~o ~' Finni Adult Mortality \\..... \\.... \\_ \\..... \\.... X for J'" l.lroods ')(_ ~ ~ /<- -,c..... ~ "><.. 7<-- X-i\\'01,*: Adt1lr D'IOn3hly (L

• hvc. 0
• dt:~). SO ""' sphl brood (smsle brood splil between two d:r.ys). CO - any O\\'c.r (offspnny earned o"c.r '"h ;iduh dunns tnn.sfer).

Co11ce11rrntio11: % Mortality: I (")1. Mean Offspring/Female: I ~\\.\\o CONC: 100% Intake Survival and Reprod11ctio11 Data Rcnlicatc number Dny I 2 3 4 5 G 7 8 9 JO I Young produced 0 0 0 0 0 0 0 D 0 l\\ Al.lull mortnlity L L L L '.? \\'oung prod uced ("') n A n ("") (') 0 0 0 () Adult mortnlity L. '- L- \\.._ L L \\...... 3 You ng produced D () 0 0 c () 0 0 n G Adult mort:1li1y L L L L L L \\.._ '- L 4 Young produced I.,. l.o s '--\\ '4. ~ s ~ s Adult mortnlity L \\.._ I..._ 5 \\'oung produced ' "?:> I\\ -\\. '"L "L.. \\ 1... l '-l lL.. '~ \\ \\ Adull morrnlity L \\.._ \\__... '-- ~ '- \\_... \\.... G Young produced u () {) () 0 0 0 0 0 0 Adult mortality L L L L L L- \\.... L 7 Young produced \\,.., l\\ \\ C\\ \\ \\~ \\ '\\ \\cs, 1 \\o ' no Tolnl you11g produced ~& ~\\..\\ ~\\, ~""'\\ ~s ~\\ ~\\ 1:,1.. ~'\\ ~"L Finni Adu ~ t Mortality \\..... \\.._ \\_ L. ~ L \\,,_ .\\'n1c: 1\\duh mon.;1Jity (L z Jive. O

• dcd), SO
• 1pl11 brood bm£IC brood 1pht between two days). CO
• C"1m' over 1om~

~med O\\'Cf wrlh oiduh dunne irlrW"cr) Co11ce11rrntio11: % Mortality:

01.

Mean OITspringlfemalc: ~~.(,, % Reduction from Control-2: - '\\.$1. SOP AT I I - Exhibit ATI 1.2, revision 11-01-14 Page 41of94 ~ (Q CD ~ 0.... co ~ EmironMt nt"I T0t.Ung 5olu1Jon'lo. Inc. Conl.-ol-1 lluy I 2 J I 0 0 0 l 0 0 0 3 0 0 0 J J 6 J s 10 II 10 6 0 0 0 7 16 16 14 Totol JO JJ 28 I0.7% ll") I 2 J I 0 Q 0 ? 0 0 0 3 0 0 0 J 5 .1 s s 12 l<I 12 6 0 0 0 7 17 18 14 Tomi 3J J6 31 2 1.4% Uoy 1 2 J I 0 0 0 ? II 0 0 l 0 0 0 J s (1 4 5 IJ 1:; 11 6 0 0 0 7 19 17 15 Tolul 37 36 JI 42.8% Uny I 2 J I 0 0 0 2 0 0 0 J 0 0 0 J J s 5 5 12 13 12 6 0 0 0 7 19 Ill 20 Tol*I JS J6 J7 Ill' 1licu ll' uumlJcr J s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 s 5 5 J 12 11 13 10 0 0 0 0 13 15 17 16 JO 32 35 JO Ht111ic:11r uumlJ'-'r J s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 3 ,j .j II 13 l'I 12 0 0 0 0 18 17 13 16 JJ J3 JJ J? lt t-nlicnl~ numbtr J 5 6 7 0 0 0 0 u 0 0 0 0 0 0 _g 4 4 5 .j 1:; IO 13 12 0 0 0 I) 16 18 16 15 JJ 32 JJ JI n~ )licnll' n111nbcr J 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 4 4 5 12 14 10 14 0 0 0 0 15 15 IQ 16 J2 JJ JJ JS T VA I Seq uoya h Nuclea r Plant, Outfall IO I May 02-09, 2017 Verification of Cerint!apf111ia Reproduction Totals 85.6% 8 9 10 Totnl Duy I ? J 0 0 0 0 I 0 0 0 0 0 0 0 ? 0 0 0 u 0 0 0 J 0 0 0 5 l s 46 4 6 s s 10 13 ti 112 13 12 14 0 0 0 0 G 0 0 u I? 14 16 ISJ 7 IS iK 15 J2 JO .)2 Jll 1'0101 J7 JS 34 100% s 9 10 Toll11

00)

I 2 J 0 0 0 0 I 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 J u 0 0 4 5 5

• 14 J

4 s 5 2 II 12 123 5 13 1.1 14 0 0 0 0 6 0 0 0 <) I) IJ 161 7 21 17 18 J; JI JI J 28 1'01~ 1 38 J6 37 Control-2 8 ? 10 Tomi Dny I 2 J 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0

• I

.j 6 4(1 4 4 4 4 10 11 IJ 121 5 12 12 13 u 0 0 0 6 0 0 0 19 17 17 IC.9 7 17 15 IS J.) .ll 37 JJ6 Totnl 33 31 )2 !Oil% Intake 8 9 10 Toto I O*y I 2 .) 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 J 0 0 0 6 5 s 48 4 6 6 s 13 12 13 125 5 13 I I 14 0 0 0 0 0 u u 15 19 IS 171 1 19 17 17 JJ JG JJ J44 Totnl J8 3~ J6 ltcolle111c numbet Toto! 4 5 6 7 K 9 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 4 6 s s 5 4 51 IJ I I IJ 13 13 14 II 127 0 0 o I o 0 0 0 0 19 17 19 I 20 IS lO 17 181 .)8 n JS I 38 J6 J9 J? JS9 ncullnk 110mbt!r Tomi 4 5 6 7 8 9 10 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 0 0 0 0 0 0 0 6 s 6 (> s 6 .1 n 12 14 13 l*I 11 I! 11 129 0 0 0 0 0 0 0 0 IS 18 20 19 19 w 17 187 36 37 39 39 JS 38 .l3 3611 llcnlicntc num)er To1ol J s 6 7 8 ~ 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 6 s 4 4 4 s 4 J 4 13 II 12 10 10 10 II 11*1 0 0 u 0 0 () 0 0 15 18 15 14 IS 15 IC. 158 JJ J4 JI ?8 J2 JO JI 316 Ht111ictllc num brr Totnl J 5 6 7 8 9 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 6 4 J 4 5 5 s so 12 12 12 14 12 IJ 11 124 u l1 0 u 0 0 0 0 19 19 15 19 15 lb 16 172 J7 JS JI 37 J? 34 32 346 ..f -r Environme ntal Te sting Solutlons, Inc. Concc 11 lrntion (%) C()utrol - I 111.7% 21.4% ~?.8 % 85.6% 100% '0111rol - 2 IUO% l111akc Outfoll 101: llu1111et1'

• i\\I 'D v:1luc; l'MSD:

Int *kc: llu 1111c11's ~ I S i),.,,1 11~ : l'M **n : I 30 \\4 37 35 37 3 33 38 2 3 33 18 3r, JI 36 31 36 37 35 34 36 37 31 32 J4 J6 2.113 6.8 1.663 5.3 TVA I Sequoyah Nuclear Plant, Outfall 101 May 02-09, 2017 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Ceriodup/111i11 dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method I002.0 Quality Control Verification of Data Entry, Calculations, and St*1tistical Analy cs rrojcrt number: _ _ _________ 12_J_J_2 Reulicn tc num ber Sunri\\'nl ,\\ vcrngc reproduction CotffiritrH or f'rrcrnl rrtlnc1ion from (%} (orr, prini:lfcrnnle) v"ri:uion ('Y*) 1moh."4.I ron1rob (*!.) ~ s 6 7 8 9 10 30 J2 35 30 32 30 32 JOO 31.2 GA Not appliruble 3J JJ 31 '2 J5 I 31 100 32.11 5.7

• 5. 1 33 32 34 J I 33 32 37 JOO 33.6 6.9

-7.7 32 JJ 33 35 J4 36 33 100 3H 4.8 -10.J 38 J_ 38 38 36 39 32 JOO 35.9 7.1 - I S. I 36 37 39 39 35 38 33 100 36.8 S. I -17.9 34 34 31 28 32 30 31 100 Jl.6 5.8 Not "lllllic:i blc 37 r JI '7 32 34 32 IOU JM 7.0 -9.S MSD = Minimum Signilicant Diflerencc PM D"' Percent Minimum ignificant Difference PM Dis 1.1 measure oftest precision The PMSD 15 thc nnmmu111 percent d11Tcrcncc between lhc control and lrcmment that con be declared stu11st1cnll s1gnilicant in a whok clllucnt toxic1ty test. Lower l'MSD bound dctcnn111cd by USEP/\\ ( 10 11' pcrccntlk) = 13% Upper PM D bound dclcnnincd by SEI'/\\ (90 11' ~1ccntile} = 47% LO\\ er und upper l'MSI) bounds were determined from the I 0th nnd 90th pcrccnlllc. rcsp..'Ct1vcly. or PMSD datu from EP/\\'s WET !ntcrlaborutory Variability tudy(USEP/\\. 2001a. USEI'/\\. 2001b) llSEPA 2 Ill. 2rHll I, Fi11:1I Report l111crl11bomtmy V:1rinb1hl r Study r EPA ShC1n-tcrn1 Chronic mid Acute Whok Efllucnl Toxicity Tc~l Methods. Vtilumcs I and 2-1\\ppcndi ~ El'A-821 01-004 nmJ EP/\\ - ~2 1 -B 05 LI, Environmcmul Protection Agency, incmnati. M. tnvl~H1~tf~l1119 SoMlori~ S1a11 Date. 51212017 TV A I Sequoyah Nuclear Plant, Outfall 101 May 02-09, 2017 Statistical Analyses Ceriod:.pnnia Survival and Reproduction Test-Roproduction Test ID: COFRCR Sarnple ID: TVA I SQN OuLrau 101 End Dale: 519/2017 Lab 10. ETS-Envir. Tesling Sol Sample lype* OMR-Oischarge Monitoring Repon Sample Date May 2017 Pro ocol. Fll\\iCHR-EPA-821-R-02-013 Test Species: CD-Cenoaaphnia dubia Comments: Non-Lrealed Cone*% 1 2 3 4 5 6 1 8 9 10 Con lrol-1 30.000 33.000 28.000 30.000 32 000 35.000 30.000 32.000 30.000 32.000 Co.... irot-2 33.000 31.000 32.000 34.000 34.000 31 000 28.000 32 000 30.000 31.000 10.7 34.000 36.000 31.000 ~4. 000 33.000 31 000 32.000 35.000 31 000 31.000 21.4 37.000 36.000 31.000 33.000 32.000 34 000 31 000 33.000 32.000 37.000 42.6 35.000 36.000 37.000 32.000 33.000 33 000 35.000 34.000 36.000 33.000 85.6 37.000 35.000 34.000 38.000 32.000 38.000 38.000 36.000 39.000 32.000 100 38.000 36.000 37 000 36.000 37.000 39.000 39.000 35.000 38.000 33.000 Intake 38.000 34.000 36.000 37.000 35.000 31.000 37.000 32.000 34.000 32.000 TrQnsform: Untf'ansformed 1-Tailod Isotonic Cone**/o Moan N-Moan Moan Min M:>x cv~. N t*Stat Crltlcal MSO Mo~n Conlrol-1 31.200 0.9873 31.200 28.000 35.000 6.375 10

34. 117 Control-2 31.600 1 0000 31.600 28.000 34.000 5.816 10 10.7 32 800 1.0360 32 800 31.000 36.000 5.713 10

-1.732 2.287

2. 113

34. 117 2 1.4 33,600 1.0633 33.600 31.000 37 000 6.902 10

-2.598 2.287 2.113 34.117 42.6 34.400 1.0866 34.400 32.000 37.000 4.766 10 -3.463 2.287 2.1'3 34.117 85.6 35.900 1.1361 35.900 32.000 39 000 7 126 10 -5.087 2.287 2.113

34. 117 100 36.600 1 1646 36.800 33.000 39000 5.092 10

-G.061 2.267 2.113 34 117 Intake 34.600 1,0949 34.600 31.000 38,000 6.974 10 Auxilia!)'. Tests St:ttistlc Critic-al Skow Kolmogorov 0 Test lndicales normal distribution (p > 0.01) 0.74361 1.035 -0.0134 Bartlett's Test Indicates equal variances (P = 0.81) 2 26348 15.0663 The con trot means are not s ignificenU~ dfflerent !e = 0.651 0.4671 2.10092 H~pothos l s Test p -1<111, 0.05) NOEC LOEC ChV TU MS Ou MS Op MSB MSE F-Prob Ounnett's Test 100 >100 2 11 279 0.06772 41 9367 4.2685.2 1.0&06 TrA~t mnnf$:. v~ C;nn t rnl~ 1 Lino;ar lntorpol:>tion (200 Resamplos) Point so IC05 >100 IC10 >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 <0 10 5 0 N g ~ 0,, c; 0 0 0 0 Page44 of94 95% Cl Skow 1.0...-------------., 09 08 0,7 0.6

i: 0.5

~ 0.4 ~ 0.3 ~ 0.2

0. 1.

0.0 -~ *., 0 --01 -0.2 --0. 3 +--~--..-~-~ ........ ~----1 0 50 100 150 Dose o/o Dose*Rosponso Plot fD ~ 0 2 .£ 1-tafl, 0.05 level of significance N-Moan 1.0000 1.0000 1.0000 1.0000 1.0000 1 0000 Kurt

• 0.8025 df

s. 5~

TVA I Sequoyah Nuclear Plant, Outfall 101 - Intake May 02-09, 2017 tatisticaJ Analyse Envlronme11talTe:st..lng Snlutlons. Inc.. Ceriodaphnla Survival and Reproduction Test-Reproduction Start Date: 5/2/2017 Test ID: CdFRCR Sample ID: TVA I SON Outfall 101 - Intake End Date: 5/912017 Lab ID: ETS-Env1r. Testing Sol Sample Type: DMR-Discharge Monitoring Report Sample Date: Ma~* 2017 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dub1a Comments: Non-treated Cone-% 1 2 3 4 5 6 Control-1 30.000 33.000 28.000 30.000 32.000 35.000 Control-2 33.000 31.000 32.000 34.000 34 000 31.000 10.7 34.000 36.000 31.000 34.000 33.000 31.000 21.4 37.000 36.000 31.000 ~:wuu

1£.UUU 34.000 42.8 35.000 36.000 37.000 32.000 33.000 33.000 85.6 37.000 35.000 34.000 38.000 32.000 38.000 100 38.000 36.000 37.000 36.000 37 000 39.000 Intake 38.000 34.000 36.000 37.000 35.000 31.000 Transform : Untransformed Cone-%

Mean N-Mean Mean Min Max Control-1 31.200 0.9873 31.200 28.000 35.000 Control-2 31 600 1.0000 31.600 28.000 34.000 10.7 32.800 1.0380 32.800 31 000 36.000 21.4 33.600 1.0633 33.600 31.000 37.000 42.8 34.400 1.0886 34.400 32.000 37.000 85.6 35.900 1.1361 35.900 32.000 39.000 100 36.800 1.1646 36.800 33.000 39 ODO Intake 34.600 1.0949 34 600 31.000 38.000 Auxiliary Tes ts Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates equal variances (p = 0.43) The control means are not significantly different !p = 0.65) Hypothesis Test (1 -taif, 0.05) Hornoscedastic l Test indicates no significant differences Treatments vs Control-2 CV% 6.375 5.816 5 713 6 902 4.786 7.126 5.092 6.974 7 30.000 28.000 32.000 31.000 35.000 38.000 39.000 37.000 N 10 10 10 10 10 10 10 10 Statistic 0.94304 1.72368 0.4671 8 32.000 32.000 35.000 33.000 34.000 36.000 35.000 32.000 t-Stat -3.128 MSDu MSDp 1.66325 0.05263 9 30.000 30.000 31.000 32.000 36.000 39.000 38.000 34.000 1-Taifed Critical 1.734 Critical 0.868 6.54109 2 10092 MSB 45 Dose-Response Plot 40 35 L-~4---f-~-t-~-r-- .2 30 -g 25 'C e 20

c.

Cl> 0:: 15 10 5 0 g c: 0 u Page 45of94 N ~ ~ 0 ~ ~ N ~ 0 (.) 0 0 co E 1-tail, 0.05 level of signfficance 10 32.000 31.000 31.000 37.000 33.000 32.000 33.000 32.000 MSD 1.663 Skew Kurt -0.2144 -0.8437 MSE F-Prob df 4.6 0.00582 1, 18 Page 6 of 7 Species: Ceriodap/111ia dubia Date: 05-02-17 Client: TVA I Seguova h Nuclear Plant. O utfall 101 Daily C/Je111istr)1: Day (Analvst idcmificd for each dav, ocrformcd oH, 0.0. and 1.:u11du1.:tivitv 111ea5u1e1ne111s only.) 0 I 2 Analyst 1'-1 J-.- tlV ct-' J-Concentration Pnrametcr ~ ~ -. -~ ~ ~..... pH (S.U.} A.21 '3 02. A.lb 01"h e..oe ~Ai-DO (m!!IL} 4-q '+..0 1-.f- --+~ ~-'1 '+.e Conductivity $Z.\\ 32.8 j(q (itmhos/cm)

• -.I ~M '

CO 'TROL

• Allmlinily

~4'\\,,. (mg CaC01/L) lo\\

• ..I

\\o1.. .I:':. "....

• Hardncs g~
• --;.~

~ .) a1-(m~ CnCO;IL) "'Temperature (°C} '"l.'-\\. lo "l~.o \\.~-"\\ '"\\.. "\\. "\\ L...\\..J "\\S. \\ pH (S.U.) e,l~ 0.0\\ All P'll'\\(') 'f>.~ '1-~~ DO (mg/L) ".)..~ ~~ ~.e t>rq ':\\-.0 ')..~ 10.7% Condnclivity __;01

31~

2o'-( (im1hos/cm)

• Temperature (°C}

"b\\*\\ "¥... \\ ~\\:"\\ -i,... \\.\\ -i."\\. ~ \\c;. *.,_ oH CS.U.l .a,q e>.01 A.\\2. /l.QG1 ~.10 '1R1-DO (m!?/L} 'l.a ~Q "")..£.\\ 'I~$-/ ':\\-.~ '*l.0 21.4% Conductivity ~~p Aq\\ 2qo 1;; (µm hos/cm) -_ c ~ Tcmncrnturc (°C) '-'\\*\\ L.~*~ \\>\\."'\\ "'\\..'\\. '\\ &.'-\\. '\\ '"\\S."1... DH (S.U.) e.1e e,.")(') '-".l'2.

iJ"ID 8.t¥'J

"'l.q~ DO (me.IL) '.}.q ~c::\\ ~.~ ~9 ':\\-.~ -':\\-.e 42.8% Condncti"i!Y itl4'5 ~" ~ (111nhos/cm) ~Si-

• .\\ -

29./) -.. ~ "T emperature (°C) "'\\.'\\-1 "?.'-\\* ~ "'\\.'\\-~ ""~' "1..~-~ 1.$. 0 1JH (S.U.) 9.\\1. Pion ~Jn e.02. >-00 ',\\_qg DO (m!?/L} '~q 1=l. q

• +.C\\

't.q ~e ~~ 85.6% Conductivity

*** - CC*

(itnihos/cm) l>lP I* lOO \\61- "Temperatu re (°C) "1.."\\* \\ L"\\-cf ,,4. '\\ I..'-\\. f "'\\.'V\\ '1.~*1 DH (S.U.) e.1z.. ~~C\\ e.111 A,l) l E'J.DS Ila~ DO (mg/L) a.o ~-" e.o qft 4t\\ PJIJ Conductivity t66 { (11mhos/c111) \\ ltl'3 lee? r- * ' ~*---

• Alkalinity

~- ~.. 100% (mgCaCO.i!L) S'\\ \\o~

• ~

~

• Hnrtlnc s

~ (mg CnCO,IL) 'b-i.. \\o\\o --~

• TR chlorine (mg/L)

<o. lO \\ ~ -. -<. O* lO "-~~-.. -:,...

• T emperature (°C)

"'t~* l "l.S.\\ L.'-\\ *., "\\..'{. '\\ -,s.o 1.'t. '1 pH (S.U.) E3.n2- "}P.~ e>.oo A..O\\ e.oco 'tq~ DO (m ~L) e.1 r+.o, {:!)J) ~.e ~.<=\\ '\\Pe Conductivity Ht>6' ~.;* \\~3 lll2.. I! (11111hos/cm) .~ ~.. -' 100%

• Alknlini!y

- ~ --~'~'

s~ ' (.\\ Intake (mg CaCOJLl

• llnrdness J

lP'-\\ \\o\\:i (mg CaC03'Ll J

• TR chlorine (mg/L)

-'.o. lO ~ ,0.10 "Tempernture (°C) '"l.4-i

l. "l. C\\

i."{. e 1.'\\.'t 1S.O ~*0 lnitfal Final lnitinl Final Initial Final

• Tempera1ures pcrfonned at the time oftest initiation, renewal or tem1inntion by the analyst identified in the Daily Renewal
• lnfomiation table locmcd on Page I. Alkalinity, hardness mid tot~I residual c 1lorinc perfom1ed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench 5hcet by: -...,M-T--

Page 46of94 SOP ATI l - Exhibit AT! 1.2, revision l 1-01-14 l l l Conccn-tration CONTROL 10.7% 21.4% 42.8% 85.6% 100% !00% lntnkc ~ r;=~/Q \\. \\-.----- ~ ll',....,.,rwl\\,fl'UlfHdnqSohlllriftt.IM. Page 7 of7 Species: Ceriotlap/111ia dubia tent: A eauova 1 uc ear er TV I s I N I Pl ant. 0 f 11 101 ut a ate: 0 OS 02 17 Ony (Analyst idcntilicd for each day __ per[om1ed pH D.O. nnd conductivitv measurements only.) 3 4 5 Annlyst V'- ,P,~1...- i>-- ~ ~ 'I-- 1)-.J A.../ Jv Pnrnmetcr ~ .:f.. ' pH (S.U.) tJ-i \\~ ~ o4 ~. n'l ~.G\\P> 8.\\~ P,,\\.)(\\ E:?.\\Or. DO (mg/L) +.~ ~-°' '"l.~ e..o ~~ a.o ~.6 Conductivity 32'--' 02.J...O . 320 311-" ( ~1mhos/cm) _j.. I~

• Alknlinity

~~.. ~ - - "x... ~~"' -. "-~ ... ~**" ~~

• -.~*

(mg CaC03'L) . ~--- -

• Hardness u- ~

~ - ~ ~ &l.. (111~ CaCOi/L)

• Tempernturc (°C)

\\.'"".~ ""t...!\\*'\\ ""\\Y\\. ci ,_ "\\. "' "1.-\\.* \\ '\\~. \\ "\\."\\.' oH (S.U.) 0.()1.o i.o'+ 'B*\\'2-Z\\.c:\\1-0.IL"'l e.oe B.\\":}. DO (111~/L) ~- e> ~ll - ~.., /?)~ ~.~ ~.o r:\\A Conductivity

i-~qt? J

~ 00~ Bco ~ _,\\lo (µmhos/cm) ~ ~°J "Temperature (°C) \\.'-\\.*'\\ \\.'S, \\ "'\\."\\.'\\ is."\\.. '1."\\. & '1.~.'\\ 1.'"\\*C\\ oH(S.U.) ~ *...a 'iS.04- '6. \\ '2. ~Al" @,o g~ S.1A DO (mg/L) "':3-Pl 8'.o ~:<> 0.0 '+(:") 0.0 ~ -'\\ Conductivity I ~ 2,qz. .2. ~\\p aoo 2'10 (umhos/cm)

• Tempe:rntur c (°C)

'i>\\*"'\\ -,,S. '\\.. -'\\.."\\.'\\ "1..C.. 'c "\\.~*~ 1..\\..:\\ 1.."l*"\\ pH (S.U.) ~ ~.o<t- ~. \\\\ J'\\,q',}- Q.10 0.<:A 0.10\\ DO (m!!IL) ... g 3.0 li'.n 0.0 r:\\_O\\ 0-0 ':} C1 Conducti\\'ily 2<.Q l ~ ~ _,) ~I03 ~51' (umhos/cm) ~ "Temperature (°C) l.'\\-'1 '1>\\. ~ "'\\>\\."\\ L').t:i '1.'\\*i ""tS.O \\..'\\,.'\\ oH (S.U.) PJ.U~ i.o~ '6.\\2... 4~":\\- 0.10 0.10 8.\\'\\ DO (mgfL) ':tA ~.\\ ~.::<> PJ..O O.n ~.o i?J.u Conducti,*ity ~'\\ \\ = '1 \\\\'.(7._... \\C\\W \\6".f (11mhos/cm) "'Tcmpernturc (°C) ""\\.~~ 1.J.\\.k "\\.'). 0 ~. \\ ~\\.f \\S.Q \\.~.o pH (S.U.) .-:}~qs ~.0$' ~.O'i-- 1.t:\\'2.. AOC\\ ~-cc-1 ~.\\'\\ DO (m!!IL) ,.+R. 'l\\.")... ')!...... e..\\ 0.0 e.o e.o Conductivity ~~. ~l.06' \\5'~ \\1-\\ 11oo (µ mhos/an) . ~ "Alkalinity ~\\ (mg CaCOi/L)

..,1. * -.

- >..A'

• .c..

"Hardness ~-~ ---- ~ -"' ~

• <' I -

~"" I (m!? CaCO;/L) l* ... ) ~' l *-ii::*: ~ ~TR chlorine (mg/L) -... ~ O*lQ

• ~

-~

• Temper nturc (°C)

~.o "\\)\\. t ~. t> "\\.'"\\.. ~ "'\\.'\\.' '1S*1 1..$.o pH (S.U.) B.oel "i! ';)$'" ~.\\\\ 0.0\\0 ~., o ~. \\\\ e.1e DO (m!!IL) () ~ <j\\.-z... 'J. a 0tJ 0.0 o,_n ei.1 Conductivity r

• p

}lP 3 llo) l'}:t ..., /""IO (11mhos/cm) 8 ~-..... _..;,_!""_

• Alkalinity t.,S

--~ (mit CaCO;IL) j ~1J,.~,-1J._

• Hardness (o~

r (mg CaCOJIL) II f j

• TR chlorine (mg/L)
• :>1

.::a.JO le

• Tcmpernturc {°C) 1$.0

'"1.Y,J\\ 1.':\\.!'\\ ~.o '1.Y.*~ '1.'i.o o.o I nit in I Fina l Initial Finni Initial Final l nitinl

• Temperatures performed al the time of test initiation, renewal or tcrminauon by the analyst 1denufied Ill lhe Daily Renewal lnfonnation table located on Page I. Alkalinity, lmrdncss and total rcsiduald\\orine p formed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:

Page 47 of94 SOP AT I 1 - Exhibit AT! 1.2, revision 11-01-14 6 .1 v-' 7 ~-01 '}A ~.. .. *I" ""'\\,C,.1.. 9,,"q ~.'! '"\\C..o A.o-1 ~.11 '\\.S..'\\ ~,i< ~ -0 ~ y I..\\.'i 0-<XP '3.o .J _. ~ "1.S.\\ 8.0 1 8.0

• ~

-~ '.;,c...:.1..;_ ~ -- "\\.'\\. \\' e.11 6'.1 1."\\ -~ Final Environmental Testing Solutlons, Inc. Conccntnition Parnmctcr lnll !SU) DO (me/Ll Conductivil\\* (11rnhos/cm) ontrol Altmlinity (1111:/L CaCO,) llartl11css (mi:/L C11COJ) Tc11111ernturc c"Cl 1>11 (SU) 00(1111!/Ll 10.7%. Cond uctil'ill' (umhosicm) Tcm11crnturc ("C) oil fSll\\ DO lmp/L\\ 21.4'Y., Co111lnrlivit1* (umhoslcm) Tcm ucratu re <C) 11111 (Slll 00 (1111:/L} 42.8'Yu Co111luc1ivi11* fo111hus1cm) Tc11111cn11urc (°(.') 11111 CSlll DO (me/Ll 8!1.6"/., ICon1l11cti"itv lumhoslcm) Tc1111>cn1111rc ("C) nil ISUl OO (mi:/I.) Co11ducti"it1* (µm hos1cm) 100% Alkn linity (mi:/L CaCOJ) ll n rdncs~ (mr:IL CaCOJ) Tomi llcsidunl Chlorine (m!!IL) Tcmocrntnrc ("C) 11111 (SUI OO (m!!/I,) Couductivitv l11mhos1cm) 100% lnt:ikc Alk.1 li11ity (mJ1L CnCOJ} ll nrtlnrss (mJ:{I. CaCO,) Tornl llcsilluul Chlorine (me!L) Tcmnrmture (°Cl TVA I Sequoyah Nuclear Plant, Outfall 101, Non-treated May 02-09, 2017 Dav o Initial 8 21 7.9 32 1 61 88 24.6 8.18 7.8 309 24.7

8. 19 7.8 291 24.7 S. 18 7.9 257 24.7 8.17 7.9 186 24 7 8 12 8.0 158 59 62

<0. 10

24. 7 8.02 8.1 163 59 1\\4

<O 10 24.7 Ceriodap/111ia dubia C hron ic Whole Effluent Toxicity Test EPA-82 1-R-02-013, Method 1002.0 Daily C hemical Analyses Day I lhv2 D:w3 Finni Initial Fi1rnl Initial Final Initial Fimtl 8 02 8.10 8 00 8 08 797 8.13 8.04 7 8 77 7 9 7 7 7.8 7.7 7.9 328 319 316 62 82 25.0 24 7 24.9 24.8 25.1 24.8 24.9 8 01 8.11 8.00 8.0R 7 97 8.06 8.04 7.9 i.8 7.9 7.8 7.7 7.S 79 314 304 310 25.1 24 7 24.7 24 9 25.2 24.9 25.1 S.01 8.12 7.99 8.10 7.97 8.09 8 04 79 7 8 79 7.S 7.8 7.8 8.0 293 290 292 24.8 247 24.9 24.9 25.2 24.9 25.2 s 00 8.1'.l 8 00 8 08 7.98 S.OS 8 04 7.9 7.8 79 7.8 7.S 7.9 S.O 265 256 26 1 24.8 24 s 24.8 24.9 25.0 24 9 24.8 8.00 S. 10 S.02 8.08 7 98 8.08 8.03 7.9 7.9 7 CJ 7.8 7.Q 7.9

8. 1 188 187 191 2.t.8 24.9 24.8 24.9 24.9 24 9 24.8 7.99 8.09 8.01 8.05 7.98 7 95 8.05 7.9 8.0 7.9 7.9 8.0 7.9 R2 163 167 165 63 66

<O 10 25.1 24.9 24.9 25.0 24.9 25.0 24 8 7.99 8.08 8.01 8.06 7 98 8.05 8.05 7.9 8.0 7.8 7.9 7.8 7.9 8.2 165 162 163 67 66 <O 10 24.9 24.8 24.9 25.0 25.0 25.0 24,9 Dav 4 lnith1I 8.08 7.8 317 63 86 24.8 8.12 7.9 298 24.9 8 12 8.0 286 24.9 &.11 8.0 248 24. ~ 8 12 8. ~ 182 2D 8.07

8) 158 67 65

<O I ~ 2'.i.) 8 11 7.9 165 65 63 <0. 10 .2-t9 Fi1rnl 7.98 8.0 24.9 7 97 8.0 25.2 7.97 8.0 25.0 7.97 80 25.0 7 97 8.0 25 I 7 92 8 I 24.8 8.06 8.0 25 0 l'rojcct number. ______ 12_3_3_2 O:n-5 lnitinl

8. 15 7.7 324 24.7 S.10 78 316 24.8 8.10 7.S 300 24.8 8.10 7.9 263 24 8

8. 10 8.0 196 24.8 8.09 8.0 171 24.9 S. 10 8.0 177 24.8 Duy 6 Fi11:1I lnitinl Fin'11 8.09 8 19 8.09 8.0 78 7.9 320 25.1 24.8 25 2 S.08

8. 17 S.09 8.0 7.9 7.9 303 2.\\.9 24.9 25.0 8 08

8. 18 8.04 8.0 7.9 so 290 24.9 24 9 24 9 8.09 8.19 8.05 8.0 7.9 8.0 257 25.0 24.9 24.9 g 10 8 19 8.06 8.0 8.0 8.0 187 25 0 25.0

• 25 I S.09 8.19 8 01 s.o 8.0 8.0 163 25.2 15 0 24.8 S.11 8.18

8. 11 8.0 8.1 8.1 166

'.!5.0 .25.0 24.K File: sqn101_050217chem.xlsx Entered by: JJmner Reviewed by: Page __ _ Page_/ _ of_ ( __ Envtronmentalli !.ting Solutlon1, Inc.. Total Residual Chlorine (ORION-1977) Meter: MalTix: Water, RL = 0.10 mg.IL Accumet Model AB250 pH/mV/Ion Meter Analyst ~I /-1. -'S----~, Date analyzed os-*oz.. *I-:/. Calibratio11: O.lOmg/L Reference stnndnrd number j)JJJ' J~~g Millivolts (mV) Sf,1. 9 1.00 mg/L /N.!.J' 1~-:rll 5* (,, lodide reagent I JNR 1-97 Acid reagent: /Nt 7?8 mV Chnngc (suggested rnngc = 26 to 30 mY) 2'1.-:/ Note: For samples with a residual chlorine of > 1.0 mg/L. the samples musl be dilulcd to be within the calibration range. ll orator11 coutro stn11 L b I d f art.: Reference standard True value (TV) number (mg/L) h.J~J J "171, 0.50 D r I up 1cate samp, e prectswu: Snmple Sample ID Sample chnrncteristics number \\~1. b~ {l..'lf:I. Rik Jn.,, r.~ j Duplicate Sam le 111ens11reme11ts: Sample Sample ID number Reaacnt Blank ~ . o l1t:J::;D I. Q 3 Mensured value (MV) % R = MV / TV x JOO (mg!L) (acceptable range : 90 to 110%) (l).5'16 loet. 2 7a Residual chlorine % RPO = {(S - D) /l(S+D)/21} x 100 (me/L) s I. 0 -000 D l. 0 -00L-f Snmple characteristics (acceptable range =+/- 10%) /'T.f' O&.H.z,.:T Residual chlorine (m /L)

l. O-oo"l l c). 003 O -ooJ o. ooJ L. 0. 002 l 0

- 0t.)} t ().c:J()J 1 o.ouJ Note: All samples were analyled in excess of EPA recommended holding time ( 15 minutes) unicss otherwise noted. L b a ora ory coutro sta11 d I arr.: Reference standard T rue value (TV) Measured value (MV) % R = MV I T\\i x 100 number (mg/l) (rng/L) (acccpta blc range= 90 to II 0%) />.J.sJ Jl..q~ 0.50 0.c;H JO? *" /(.? Reviewed by I ~ Dmc revit:wt:LI 3*02..11-Page 49 of94 OP C8 - Exhibit CS. I, revision 01-0 l-13 I l I I I I I I I I I I I I I I I I Page C Page_2_of~ Envlronment-,J Testing Solutions, Inc. Total Residual Chlorine (OJUON-1977) Analyst 1.-/-f-J-----., Date analyzed OS'* o 1., ~ Meter: Matrix: Water RL "' 0.10 mg/L Accumet Model AB250 pH/mV/lon Meter Iodide reagent: Acid reagent: Calibration: 0.10 mg/L 1.00 mg/L mV Change (su csccd rn 11gc = 26 10 JO mV . the samples must be diluted 10 be within the calibration range. Laboratory control ta11dartl: Reference standard number True value (TV) (mg/L) 0.50 /-(/JD l I -:J- % RS = MV I TV x 100 ample chnracteristics Residunl chlorine (m /Ll %RPO= f(S

• 0) /l(S+D)/21} x 100 (11cccpt11ble rnngc ="' 10%)

Duplicate S I amp. e 111easure111e11ts: nm pie Snmple ID number Reagent Blank \\1oSDZ.. \\) \\'JA\\Pc!. l~I\\\\({ -~tLe. / I / /-- s Lo. l)J"f D L o-ol Z. ,.....;i i---------- o.rvzr,. Sample characteristics Residual ch lorine (mg/L) ,..~ ,..)"'tJ?.I? ' P:dt..k ( p.§&'1 ('). oO~ -/ / / / .A A f1 / I ( / ---,_ 0 '-...J'._, i Note: All sampl s were analyzed in excess or EPA recommended hold mg lime ( 1 S mmutes) unless 01hcrw1se 1101ed. laboratory control standard: Reference standard True value (TV) number (mg/L) /JJ.J.J' h 11J 0.50 Measured v:iluc (MV) (mg/LI % RS = M V I TV x 100 (acccplllble rnngc

• 90 to 110%)

Reviewed by I JJ Date reviewed ()5. ozA1-Page 50of94 SOP CS - Exhibit C8. I. revision 01-0 1-13 EnYlronmenlaf Testing Solutions, Inc. Page _.....__ Page _ I_ of _ '2_ Total Resillual Clllodue (ORIO -1977) Meter: Analyst...., 1-1. -* -'J-------., Matrix: Waler, RL = 0. I 0 mg/L Accumet Model AB250 pHimV/lon Meter Date analyzed O ':>*D"T* /"l lod i~e reagent: ~_,_J_Al_fi--'-1-_"7_1 ___ -l Acid reagent: L--. _l.:..: >.J_,fl'---7-~ '19 Calibratio11: 0.LO mg/L LOO mg/ L mV Change (suge.cstetl r:lllJ?C = 26 10 30 m VJ Reference standard number I }.,J.tJ 1'1 ?-8 /,41./.J' l"t'f'I M illivolts (mV) S":/2 *7' foe>). 2

28. fJ Note: l'or samples with a residual chlorine of > 1.0 mg/L, the samples musl be diluted to be w1l111n the cahbra11011 range.

laborator,,- control standard: Reference sta ndard True value (TV) number (mg/L) /ijfJ l'-t ;rg 0.50 D I up/icate samp e precision: Sample Sample ID Sample c ha racteristics number no~o'-\\.o\\ A~ f.. r~,, *- rP~ -..1 1 Duplicate ~ Sam le 111eas11re111e11ts: Sample S11m plc ID number Rea ent Blank \\ i o~ o'-\\. IT!:> M easured va lue (M V) % RS = MV I T V x 100 (mg/L) (accepta ble ra nge= 90 to 110%) Q.Q)3 }00-6 ~ Resid u:1l chlor ine % RPO = {(S - D) /(( + D)/21} x. JOO (mg/LJ s Lo.ooC. D L,,. oot Snmplc chnrnctcristic (ncccplnblc range - +/- 10%) 0 .rt:1'?1'~ Residu11I chlorine (010/L) - o - 0 0'$" O

• ooz L o - 003 0 - oo -Z 0 - 0 17 0 - 00 l V - Ot> }

L o -vo* Note: All samples were analyzed in e:.:cess of EPA recommended holding time ( 15 minutes) unless otherwise noted. E;\\o\\et~ll P1'i~o~~*\\'"\\ Lb I d d a oratory coutro stan ar : Reference

• tanda rd T rue value (TV)

Measured value (M ) % RS = M I T V x 100 number (mg/L) (mg/L) (acceptable rn ngc = 90 to 110%) I )J r,1 '"'17r8 0.50 /'n,~ ]"1.k//'v Reviewed by ~--ai{.._ _ __ ---l Date reviewed '----'O:o.....oSoc...*..;:;lM_,_* J,_1...._ _ _. Page 51of94 SOP CS - Exhibit CS. I, revision 01 -0 1-1 3 Page _.....___ Page~_ of £nvlronmentall6tlng Solutkms# hu:. Total Residual Chlorine (ORION-1977) Matrix: Water, RL = 0. I 0 rng/L ---.Meter: Accurnet Model AB250 pH/mYnon Meter Analyst IHJ I Iodide reagent: I........_ rt/' I Date analyzed 05. O'-'/* / 3 Acid reagent: : ~ 0 Calibration: Laboratory co11trol stmulnnl: Reference standnrd number D /" I up. 1cate snmp e prec1s1011: Sample Sample ID number \\""\\oSo'-\\.CS Dll~<<.(\\~ L Duplicate s amp e measurements: 0.10 mg/L True v11lue (TV) (mg/L) 0.50 Sample characteristics PCl-4. h. rP1J11. t;,,J,?IP.; ample ample ID number Rea1?ent Blank 1.00 mg/L Mca ured value (M (rng/L) m Change ( u2gcstcd ranJ!c = 26 to 30 m V) Resid ual chlorine %.RPO = {(S - D) /l(S..,.D}nl} x 100 (mQ/L) (acceptable range =

• 10%)

s 0.1z.B D o.1'l.(,. /.',0 Sample chnractcristics Rcsidu:il chlorine (mii/L) I"-'(~ ~,..> t,0ScN.6f t\\.nru>1\\eA~ u lfl l l,n, )/,;,AA C>. O<>{, CJ 7 I I I t-1 I I I riJ '-Ql-// 1-- v I I

• ~

Nore: All samples were analyzed in excess of EPA recommended holding tune (15 mmutes) unless otherwise noted. laboratory control standard: Reference standard True value (T ) Mensurcd v:ilue (MV} % RS = MV /T x LOO number (rng/L) (mg/L) (acceptable range = 90 to 110%) /;)JJ I '-1??i 0.50 o. 481 9'- 2.jlo. Reviewed by I J'.I Date reviewed ::::o:'#-rs............ -~o:'i:-:1-1~-----.....,~ Page 52of94 SOP CS - Exhibit C8.l, revision 01-01 -13 I I I I I Page t l Page of Env ronmcmt;.I Testing Solutlont.. fnc. Total Residual Chlorine (ORIO -1977) Meter: Matrix: Water, RL = 0.10 mg/L Accumet Model AB250 pH/mV/lon Meter Analyst [....-J.....J-- [ -~-v-___,, Date analyzed hS.t>>. *"l-Calibratio11: O.JO mg/L Reference standard number 'NSS1 Millivolts (mV) 5 t.00 mg/L lod i~e reag. ent: [IN l-3o/l Acid reagent: ~ ('l rl -=1"8 --"-=------' mV Change (SUJ?l?CSICd ran c: 26 fO 30 mV) Note: For samples with a residual chlormr. of > 1.0 m L, the 5amples must be diluted to ut: wilhin the c libration range. laborator

• coutrol standard:

Reference standard number Du licate sam 1/e 'Jrecisio11: Sample Sa mple ID number noso c...o Me...y._ ~ Duplicate Snm 'J/e meas11re111e111s: T rue value (f V) {mg.IL) 0.50 Sample characteristics Sample Sample ID number Reaoent Blank Measured value (MV) (mg/L)

o.

Residual chlorine (m IL) % RS = MV I TV x I 00 (accepta ble rnngc.. 90 to 110%) % RPO = {(S - D) /f( + D)/21} x 100 (ncccptnblc rnngc =;!; 10%) ample characteristics Residunl chlorine ( m ~/L) O.c\\\\p < O.OQ.... b.co-:r <.:> *.QO\\ Note: All samples \\\\Cre analyzed in excess of EPA recommended holding time (IS minulcs) unless otherwise noted. laborator co11trol sta11tlnrll: Reference stnndnrd number True value (T V) {mg/L) 0.50 Measured vnlue (MV) (mg/ L) % R = M V I TV x I 00 (accepta ble ra nge

• 90 lo 110% )

q Reviewed by I j{ Date re iewed ~==o:.s::-:o~lo==~ :n====~ Page 53of94 SOP CS - Exh;bit CS. I, "vi,;o, 01-01-~ _ J l l l l l l l l J J l J J J J G A'.O?,.,(;- §,,,*.~ full ~

• * "J

~.,a= ' "-1' . :~

• o

.*.. 1 tr:* ~.: I * -;~ 0

~ !

:t L _

.-JJ

_J '..._../ 5 EnvfronmtntillTuting 5otu1ions.. Inc:. Analyst I \\Iv Date analyzed 1--,.,0:-~-,.-* 2-6-.\\-')-- -1 Alkalinity (SM 2320 B-1997) Matrix: Water, RL = 1.0 mg CaCO,IL Titrate samples to pH=4.5 S.U. Page 3(p Page ___ of _ Time initiated I r I 05 Time completed===( :12==2-======== Titra11t 11orma/ity anti multiplier tleter111i11atio11: pH of Normnlity Normality (N) ofH1SO~ pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Nn2C03 x 0.05)/E = (N x 50000)/ 100 ml snmple water reference standard ml ml ml "' 0.25/E =Nx 500 =4.5 s.u. number number (E) (acccp1ablc rnngc = 0.0180 - 0.0220) 6.-+ lNRu'-t l ~~S.l'58'i o. '2.. l2.'-I 12.L. o.o-i.o 5"' 1\\..J.2.. tlll(...Wf\\N.t..1 - o. - 0.'2. :.o. l a oratorv co11tro slam art: Lb I CJI I Reference standnrd True value Sample Alkalinity (MV) %RS = MV / TVx 100 number (TV) volume Begin End Total Multiplier (mg CnCO;/L) (acccptnblc range (mg CnCOJ/L) (ml) ml ml ml = 90 to 110%) I NS.S\\55~ 100 100 12-'*4

{~.!I Jo.z..

(b.1-(DY llY**I. (/). D r l up. 1cate samp, e prec1s1011: Snmple Alkalinity %RPO = Sample Snmplc ID volume Begin End Total Multiplier (mg C:iC03'L) {(S

• D) /l(S+D)/21} x I 00 number (ml) ml ml ml

(:icccptablc range = +/- 10%) n ~-;;i:an.SSw f UO M1.1a ~b-0 ~.'-f lo.z. s 35 --.*-* ~---!~:: _,.:.";'.:....... :~.. .... _, ** !r * *: ". j Duplicate (B) ...l ~ l4. '> act.'1 2.v( J DJ~ -O'-<"ZeJJ7 Mi 'k alrtx S!Jt *e recoverv: Reference st:mdnrd Spike vnlue Snmplc Spike alkalinity (A) number (SY) volume Begin End Total Multiplier (mg CaC03/L) (mg C1C03'Ll (ml) ml ml ml I ~~.SI~~ 6D \\ L)O ~-0 oLL'I e.~1 ~o.z. eLJ Sample :'lll<allnlty (B) Measured s11ilce value (MY) % R - MY I SV x 100 (mg CnCOJIL) MY = A - B (:icccpt:tblc rnngc (mg CnCOJILJ = 75 to 125%) 9; '1 a, Cf ei.in. s l amp e 111eas11reme11ts: ~ Sample volume Begin EPd Total Alkalinity Samole\\Buui'ber Sample ID (ml) ml ml ml Multiplier (mi;: C:iCOJ/L) Ol.t*~:n SSLiU 100 ~~.1.1 '.31. e.;. \\..\\

• O:i

!>5 19 '-1

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fn Hsw I q,1.e ~'-l.I l;.3 ~~ o4-t a.rtA I 1,1 i *' lo. "?I ~v( 0'1*,q.11-12> ~.l 14d v.o ~{ 04* ~(/Jn(.} IY.1 ~0.1 (d.O (t1 I O'+OMn b ~o.l ~ll.Ol-to. I [4 z.. (J '::4 *8. (o.fT fl 0V:i.SvJ ~tt. 2.. 3~.o 'O. 1 C/o O.~* 'd.*b.11* (6 ..1 3~.o t.l4.o Q.o qz.. j ~,. 42en -~ Reviewed by: Date reviewed: j 6$*0\\*\\"'1 Page 54of94 SOP C6 - Exhibit C6. I, revision 01 13 l l l l l l l I J J J I - Analyst I Ssv Date analyzed :=:0:0:.:01==*\\=:1:===== Alkalinity (SM 2320 B-1997) Matrix: Water, RL = LO mg CaC03'L Titrate samples to pH= 4.5 S.U. Page 31 Page_~_of S Time initiated I ':::: Time completed :=:l!.L:.,,:... ::======~ r: I /"/' f 1trn11t norma 1t11 am 11111 ttp. ter r.etermmnt1011: pH of Normality Normality (N) of H2SO~ pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Nn2COJ x 0.05)/E = (N x 50000)/ 100 ml snmple water reference stnndard ml ml ml =0.25/E "" NX 500 =4.SS.U. number number <El (:icccplnblc r.ingc = 0.0180

• 0.0220)

~.7.- 1Ne..~~ S,..1'$ IS"i:'l-O.Y. 1'2..<Z 12..4 0.0 'Z..t'.Y2-JO.\\ a orn ory co11 o slam an; Reference st:rndnrd True vnlue Sample Alkalinity (MY) % RS = MY I TV x 100 number (TV) volume Begin End Totnl Multiplier (mg CaCOJ/L) (ncccplnblc range (mg CnCOJ/L) (ml) ml ml ml = 90 to 110%)

r \\JS'.:> l?S-3 100 100 1'2. ~

2'2..~ In o \\0. \\ }\\::11.0 IDl.o 0 /.:> D l" I up, rcate samp. e prec1s1011: Sample Alkalinity %RPO = Sample Snmple iD volume Begin End Totnl Multiplier (mg CnCOJ/L) {(S

• DJ /f{S+D)/21} x 100 number (ml) ml ml ml (ncccptnblc rnngc "': 10%)

s o;.tt-\\.A-SSiv0 }II() n.i

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3.?, \\o. \\ 33 ..J Duplica1e (B) J_ l-- D e;.'"' 0'5... 1 l1 2.lP.\\ zq."+ 3.3 33 M "k atnx spt e recovery: Reference standard Spike.value Sample Spike nlknlinity (A) number (SY) volume Begin End Totnl Multiplier (mg CnCOJ/L) (mg CnCOJ/L) (ml) ml ml ml -:}:'~SS \\'33? ~loo 100 "Z.(o.. \\ 3'\\.~ 13.'2. \\0.\\ 13D Sample all<nl n ty (B) Measurcu spilce value (MY) % R - MY I SV.x 100 (mg C:iCOJ/LJ MV=A-B (ncccprablc rnngc (mg CnCOJ/L) =15 to 125%) 33 'i1- '1'°:J'.D0 1c s m11p, e 111ensureme11ts: Sample volume Begin End Total Alknlinity Snmole number Sample ID (ml) ml ml ml Multiolier (mg CaCOJ/L) 06.t)2..r:t m \\+.::vJ \\O'O ~<i.~ IJ..1.5.'S

w. '2.

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• 1 I

r .I J J I Analyst! ~ ~ Date analyzed DS:;;-;r

• ij Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to pH=4.S S.U. Page 3 <i Page ~ of 5 ~-- Time initiated F f~Pt Time completed S T: I I. r l r 1frm1111orma 1tv mu, 11111 ltp 1er t etermma/1011: pliOT-r-- Normality Normality (N) of Hlso~ pH Factor or Multiplier Deionized Titrnnt r--.. Rea in End Total = (5 ml Na2C03

• 0.05)/E

= (N x 50000)/ 100 ml sample ~.. water reference st:rndard ml = 0.25/E = Nx500 "" -- Cnccc"}1t:tble.i;ane:c = 0.0180 - 0.0220) =4.5 s.u. number number (E) ...;..0501o<T Lb l I a oratory co11tr o sl am an: Reference standard True value Sample Alkalinity (MVY % RS = MV I TV x I 00 number (TV) volume Begin End Total Multiplier {mg CaCOJJ'L) (acccplnblc rnngc (mg CnCQ3/L) (ml) ml ml ml .. 90 to 110%) J~S \\SS~ 100 100 -"?-. -=r-l~'l> 10.1 JO.\\ l0'2.. 102.-, 0 Oto D r l 11p tcale samv e pr ecrs1011: Sample Allmlinity %RPD= Sample Sample ID volume Begin End Total Multiplier (mg CnCO.i/L) l(S - 0) /((S+0)/21} x 100 number (ml) ml ml ml (n~ccptab l e range c :f: 10%) 8""" eMu f-1 lY"\\ s 1**-:..-* *

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• ).1::\\-

9.o lo.I '11 Snmple alkalinity (B) Me:1sul'ed spike v:ilue (MY) % R - MY I SY x 100 (mg CnC03'L) MV=A-B (acceptable rnngc (me: CaCOJIL) .. 75 to 125%) 3q 52 tc't. o l>jo S I amp e meas11re111e11ts: Sample volume Begin End Total A lkalinity Samole number Sam11le ID (ml) ml ml ml Multiplier (me. CaCO.i!Ll \\\\05.lfu. ~~ -:rt.Hv fun 2-100

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• 0.0220)

,~.. Lb I l I a oratory coutro stmu art : Reference standard True value Sample Alkalinity (MV) % RS = MY I TV x 100 number (TV) volume Begin End Total Multiplier (mgCnCO.v'L) (acccplnblc range (mg CaCO.i/L} (ml) ml ml ml = 90to110%} "f t::PS \\S'S :S 100 100 3.\\ 13.1.- j'D.\\ Jo.I \\oG.. \\02..01:>~ D /' I up, 1cate samp e preCJs1011: Snmple Alknlinity %RPD = Sample Sample ID volume Begin End Totnl Multiplier (mg C:iCO.YL) {(S

• 0) /((S+0)/21} x 100 number 1V'A (ml) ml ml ml (ncccplnblc range ~ +/- 10%)

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~.o lltO ~ 06°0"1-!1-atrtx S/Jl e recovery: Reference st:tndard Spike value Sample Spilce allcnlinity (A) number (SV) volume Begin End Tot11I Multiplier (mg CaCO.YL) (mgC:iCO.i/Ll (ml) ml ml ml ~\\..lSS lSS3 100 ~a; 50 2.)."> ~ 13.0

z.) 10.\\

'2..1..e c Snmple allrnlinity (B) Measured spike value (MY) % R "" MY I SV x 100 (mg CnCOJ/L) MV = A-B (acccptnblc rnngc (me CnCO.i/L) =75 to 125%) llPO

2;,., ""'"" ') * "

\\OC \\eo.c"'/o I s nmp. e 111eas11reme11ts: Sample volume Begin End Total Allcnlinity Sample number Samole ID (ml) ml ml ml Multiplier (m!! CnCO.VL) r1 O.S\\Th * ~o -fJAAt r-vp 5o 3~.'5 ~"I(. ~ ).~ 'ti.. ') \\'C). \\ 3<.. \\10~61.'\\ j I Ni I 6'X.~ L..IJ,I.\\ S.K l'Z \\It l~O?J.20 rnM1w 2.5 l),Q 3.'1 .~.q II.+) \\loo \\' c.s. o-i- <lS QN. c-n ho c.U. So .3.'I -:}.o ~\\ ll1. \\ ,, a. ~ 6ct OS*~ \\1 oSo\\.~ --r.JA~N \\O\\ \\ +.t> 'l.1 ~!=! L'L\\ ~I ~~.";f; I

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-=i.q 13.0 3,\\ I">,'\\ k '2, \\4C\\So'S. \\\\ J 21 13*0 flD.3 .'l,.~ (,-i.-) ~-:r 1\\0So\\.ti.J. '1.Jlt S'Qr-> 'Q I 1,.JT l llt*"i 14..1 l).'l (;). '\\ ~ '5G\\ o-s~ \\\\ 0 S.<P.>*1>\\o ~ 2 1q:i O:Z~.5 3.~ c.,,...\\ - Vt Reviewed by: Date reviewed: j ~c_, -01* \\" Page 57 of94 SOP C6 - Exhibit C6. l, revision 01-01-13 ~~~~~~~~~~~~~~~~~~ ~ l l l l l ] .1 I ...J _J J J I I Analyst j f?Su Date analyzed._f-_- _... 0~6:-~1:>_~---~*-\\'1-~-:_-_-i~ Alkalinity (SM 2320 B-1997) Matrix: Water, RL = 1.0 mg CaCOJ/L Titrate samples to pH=4.5 S.U. Page ~o Page !.-{ of 5 - - - Time initiated i-:::.--.._~"T Time completed I Titraut normality and multiplier tfeter111i11atio11: ~ Pu v. Norm3lity Normality (N) of H2SO~ pH Factor or Multiplier Deionized Titrnnt check Total (5 ml Na2C03 x 0.05)/E (N x 50000)/ 100 ml sample water reference st:ind:ird ml ml lnr-~5/E =Nx500 = 4.5 s.u. number number (E) ( p b =0.0180 - 0.0220) .. u~tol\\ Lb a oratory contra I t I I Sant art: Reference stand:ird True vnfuc Snmple Alkalinity (MY) % RS = MY I TV x 100 number (TV) volume Begin End Tot:il Multiplier (mg CnC03/L) (ncccplnblc r:ingc (mg CnC03/L) (ml) ml ml ml =90 to110%)

r~ 1~5:,

100 100 ~~.? =32 IT SS\\- 1:15-o~ *l ~ I t;j;;:,.o0 /.. c:i.q 11.,.k tro. I I t>t> D r I up, tcate Sffl11fJ e prec1s1011: Sample Alk:ilinity %RPD = Sample Sample lD volume Begin End Total Multiplier (mg CnC03/L) ((S

• D) /f(S+D)/2)} x 100 number

'&../IA (ml) ml ml ml (ncccplnblc range*: JO%) s -. -.,.. **.:. *~...

• ~..... *.*t*.'"*-;::.. '

no$ o~-'.t ~N IDI 1~'2. 5't> 32..4 10.\\ lO 5o 35.v Hg.l. '0.'2.. ~l \\0.1 ~ 110 Sample allmlinity (B) Mensured spil<e v:iJue (MY) %R=MV/SV.1.JOO (mg CnC03'L) MV = A - B (:icccptablc rnngc (mg CoCO.VL) = 75 to 125%) (;5 nr IOS' Ori:' " Of~ I05'JI, s amp, e 111ens11re111e11ts: o~n Sample volume Begin End Tot:il Alkalinity Sample number Sample JD (ml) ml ml ml Multiplier (m2CnCO.i!Ll \\10SQ\\.(C) ~~A ~ t0 l01 Uv \\ So (),0

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1.

35,0 l Dup!Matc (B) o.o atnx sp1 <e recovery: Reference stnndnrd Spike vnlue Sample number (SV) volume Begin (me. CnC03'L) (ml) ml 'f }-.!SS IS'~? .::<5oo ?._ o.o Snmple allrnlinity (B) (mg CnCOJ/L} 3300 s l amp, e 111ens11re111e11ts: End ml 35.o End ml 4J.'+ ~.c; End ml 11.S Normality (N) of H 2SOJ = (5 ml Na2C03 x 0.05)/E =0.25/E pH Fnctor or Multiplier = (N x 50000)1100 ml sample =Nx500 range

• 0.0180 - 0.0220)

All<alinity (MV) %RS=MV/TVx 100 Total Multiplier (mg CaC03'L) (ncccptnblc range ml = 90to110%) 11>.o fo.1 IOI \\o l. o0 (o Allcnlinity %RPD = Total Multiplier (mg CaC03'L) {(S - 0) /((S+D)/21) x 100 ml (acceptable r:ingc = :1: 10%) s --~:.:...

• • ~ -~

lt.'-+ t<n\\IO.l 3z.oO I *o\\ _l D -Ir. ~. 1°1 * (o~ 33oO 'l:l.o!... f>.<;'... ~.. ~ )n... Spike alknlinity (A) Totnl Multiplier (mg CnC03/L) ml 11.'3 ~) \\t:>.I 5l?oo Measured spike vnlue (MV) % R= MV I SY x 100 MV.. A-B (ncccptnblc rnngc (me. CnC03'L) = 75 to 125%}

<5oo joO.C) 0 /o Sample volume Begin End Total Alkalinity Snmple number Sample ID (ml) ml ml ml Multiplier (mg C:iC03'L)

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• Env!ronmtnUITasdng Solutlons. Inc.

Hardness (SM 2340 C-1997) RL = 1.0 mg CaCO~/L Analyst I lu Date analyzed._0_'1_*~-6-.-,1----1 t I l - r . r l I 1 ran uorma 11y ant mu tip, 1er t e ermma t011: Titrant Normality check Begin End Total reference standard ml ml ml number number (E) 1.tJq,.--=tQ, I NsSI 1-Nl4 l).() \\O.o ID~o L b I I I a oratory contro stam nn: Reference standard True vnlue Sample number (TY) volume Begin End (mg C:iC03/L) (ml) ml ml \\ N$S 1'-1-ef} 40 50 (D.0 \\2_o D r I up. 1cate samp, e prec1s1011: Snmple Sample Snmple ID volume Begin End number (ml) ml ml Q4*80. /). .Ssw Ob l2.o l'-t.o Duplicnte (B) ~ jvlo 1~~0 M "Ii atnx sp1 *e recovery; Reference standnrd Spike vnluc Sample number (SV) volume Begin End (mg CnCOJIL) (ml) ml ml \\JllS.S1'ltH ~o 6D 14.u \\>,u Time initiated ~~IU:"""l_.UJA ..... -=<.P.__ __ ---1 Time completed._.... _Q""'-6l.P""'-=------' Normality (N) of EDTA pH Factor or Multiplier = 0.2JE = (N x 50000)/ 50 ml sample (:icccpfnblc rnngc = 0.0180 - 0.0220) =Nx 1000 0.0200 z_o.o Hnrdness (MV) %RS=MV/TVx 100 Totnl Multiplier (mgC:iCO.v'L) (:icccpr:iblc rnngc ml = 90 to 110%) -z_o -w.o '-lo \\00 01. Hnrdness %RPD = Totnl Multiplier (mg C:1CQ3/L) { S - D} /f{S+D)/2J) x IOO ml '2.,o Zo.o s YO -Z~o j D 40 --41-.0 '-I 2e n-Spike hardness (A) Total Multiplier (mg CnCOJIL) ml ~to zo.o eo Sample hnrclncss (B) Measured spike vnluc (MV) % R = MV I SV x 100 (n1g CnC:OJ/I.) MV = A-B (ncccptnblc rn11gc (mg CnCO.v'L) = 75 to 125%} '--10 yo 100. in. s l amp. e 111eas11re111e11ts: Sample volume Begin End Total Hardness Snmole number Snmole ID (ml) ml ml ml MultioJier (mg CnCOJ/L) TV =ND,;~ Blank 5D lA I. (should be = 0 111£ CaCO.;/L) l).Q b.O o.o w.o ~ O'-{ :'A~'}. 'e:>.c> 2,).() "2.,o r '-JO 6E>W O"l.11a.n mi+sw ~Q,O "'4.ltJ 4.1.tJ Cl2.. b Y.14.11-A ~tild J.9./ '{.-;- C/o l')'{.j q.1}8 iA'1.J j.3.-\\ 4.~ 66 O\\,/.JltJ.11 A "3.J. '.) ~.'1 J.y ~ 01../ *9.0.11 r.... ~fl '-41.c - t../.1 ez.. bv <lid~- (-} 4W '-4i.8 ~.e I (.ti I v {b 41.e; 43~ O.i.o (2 J (.., 43.4 44:0 O~b 12-Note: If >15ml oftitranl is used, sample must be diluted. Reviewed by: Page 60of94 Date reviewed I 6S *o\\* t'\\ SOP C7 -Exhibit C7.I. revision 01 -0 1-1 3 l l l l l l l l l I .I J I J J Analyst I Lf'._, Date analyzed 1--Q=-~-=-.- a-6-.l-}----1 r:1 u* r l t . /' t 1 ra11 11or111a uy a11t 11111 tp, 1er t e ermma t011: Hardness (SM 2340 C-1997) RL = 1.0 mg CaC03/L Page 2. Page 2-of 3 Time initiated E 01.t*2-(9p. ---i Time completed ~ Htr..fillf Normality check Begin End Total Normality (N) ofEDTA pH Factor or Multiplier reference standard I .1 0.2/E =(N 50000)/ 50 ml sample number number (E) (:tcccptnb1e rm.,.~ u*U*~..ll22Dl =Nx 1000 v-L b I l I a oratory contro slam an: Reference standard True value Snmple Hardness (MV) % RS =MV/TVx 100 number (TV) volume Begin End Totnl Multiplier (mg CnCOJIL) (ncccptnblc range (mg C:iC03/L) (ml) ml ml ml = 90 to 110%) 1~ \\'-it)"} 40 50 4~.(J c..\\"10 z_o w.0 YO l c:xJ. ({), D t: l up. 1cate sm,,-o. e prec1s1011: Sample Hardness %RPD= Sample Snmple ID volume Begin End Totnl Multiplier (mg CnCOJ/L) {(S- 0) /((S+D)/21} x 100 number (ml) ml ml ml Q\\)Ot)Jn D 60 13.1 IY.1 1.0 s 't<J -io.o Duplicate (JU .J l <..\\. t 15.1 1.0 1 D --1.1 o\\..\\z.e~ 't-<) /; atnx spi <e recoveri1: Reference st:mdard Spike vnlue Sample Spike hnrdness (A) numb<?r (SV) volume B<?gin End Total Multiplier {mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml \\ }.)~l'/fH- '4D 6U \\ ll.I l1-o

2..t1

'ZD-0 66 Snmpl<? hnrdncss (B) Mc.1surcd spike value (MV) % R = MV I SV x 100 (mg CnCOJIL) MV=A-B (acccpcnblc range (mg CaC03/L) = 75 to 125%) -Z-0 35 QS.07, s l amp. e measurements: Sample volume Begin End Totnl Hardness S:imple number Sample ID (ml) ml ml ml Multiplier (mg CaC01/L) TV=Nn Blank (ShOUIU uc u 1111!, \\..llL.'-'.Yt..J ~A. 0 t1:uJ1r &.\\f~f-r-i; 5D 17,0 11*!1 o.ti l,b.o 1e I 0 I=" 11-.'l ll1.3 I.~ Z.8 1 ~ Ill.., 1,6 3<.o 2J. / 1-{

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• D r up, 1cate sample nrec1s1011:

Si1mple Hardness %RPO= Snmple Snmple JD volume Begin End Totnl Multiplier (mg CnCOJfL) {CS* D) /f(S+D)/2(} x 100 number (ml) ml ml ml d) lJ ll/lJ.._,.. 1\\J 26.'\\ s.3 (o 5D

j7'.':l 1.6 2.0... 0

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\\0.0 10.0 l b I I I (1 orntory co11tro slam an: Reference standard True value Sample number (TV) volume Begin End {mg CnCO.i/L} (ml) ml ml r 'N$ l'-\\<t-:r 40 50 10.0 I\\. 'i D /" I up 1cate snmp. e prec1s1ou: Sample Sample Sample ID volume Begin End number (ml} ml ml 05.cH.(} 6Sw c;-o \\).~ 1~ 4 Ouplicalc (B) _L \\~. "+- l'ii.~ atrix s11i <e recovery: Reference standnrd Spike value Snmple number (SV) volume Begin End (mg CaCOJ/L) (ml) ml ml -rLI£.<. ~ +- >-ID So l~."t r;r.w Time initiated ~\\"""J.;_~ .;;__(J?.:.----~ Time completed........_l-'4-'-'-l.-"3:-'-----1 Normality (N) of EDTA pH Fnctor or Multiplier = 0.2/E = (N x 50000)/ 50 ml sample {ncccplablc range = 0.0180 - 0.0220) = Nx 1000 n O? ()0 Zo.<> Hardness (MV) %RS = MV/TVx 100 Total Multiplier (mg CnCO.VL} (ncccpt:tblc range ml =90toll0%) I. q; 2-0.o 3\\C'.C ~.cc.lo Hardness %RPO = Totnl Multiplier (mg C:tCOJIL} {(S - D} /l(S+D)/21} x 100 ml s 1-"1 'Zo.o 3i D ---£'!1<..- ).'\\ .L 3~ os;-*.,..-4 Spike hnrdness (A) Totnl Multiplier {mg C:tCO.i/LJ ml .a.q -io.o -a Sample hardness (B) Measured spil<e value (MV) %R = MV/SVx 100 (mg CnCOJIL) MV=A-B {ntccptn blc rn ngc (mg CnCOJIL) = 75to125%) 38 es. <>5-0..... y 1.f-'40 )OO.o0 Jo s l amp, e measu rem en ts: Sample volume Begin End Total Hardness Snmnle number SamulerD (ml} ml ml ml Multinlier (mgCnCO.i/L) T V = ND Blank (should be "' 0 mg CaCO;/L) 5o o.o D.o o.o -zo.o ....w (6.a;i.11-

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" *,i ! I I I Page S ~-- Page 2... of 4 .._) Environmcnta1 Totln_g SofuUons:, Inc. Analyst I s~(... ~ Hardness (SM 2340 C-1997) RL = 1.0 mg CaCO,IL Date analyzed O'O*Ov "\\ Time initiated I "-- OSD"'~ --i Time completed. ~ Ti' I ,. r ttrnnt 11orma ity anti 11111 /tfJ, ter llelermma/1011: J a....... uu. litv check Begin End Totrll Normality (N) of EDTA pH Fnctor or MultipJicr reference stnndnrd ml ml = 0.2/E = (N x 50000)/ SO ml sample number number f--41?.l (acceptable range= 0.0 l80 - 0.0220) =N x 1000 own-- ~ Lb I I I I I a oraforp con ro s flll~ arl: Reference st:indnrd True value Snmptc Hnrdness (MV) % RS = MV /TV x 100 number (TV) volume Begin End Totnl Multiplier (mg CnCO.i!L) (ncccptnblc range (mg CnCOJ>'L) (ml) ml ml ml = 90 to 110%)

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Jv.4 2..o 2J;>. o >+o I cc.o0 b D r I up. tcate smm1. e prec1s1011: Snmple Hardness %RPD= Snmple S:implc ID volume Begin End Total Multiplier (mg CaCOJ/L) ((S-D) /((S+D)t21} x 100 number (ml) ml ml ml s \\'"lb SQ\\ *6 'l-w;:r-~~' 5o 10.4 114-.q 1-\\-.5 1..o.o 'lo I l-Duplicate (B) j_ .1-D --as, 05'.*..,,,_ IL\\-."I l'lH- ~s 'lo afrrx sp1 re recovery: Reference stnndnrd Spike vnluc Sample Spike hnrdncss {A) number (SV) volume Begin End Total Multiplier (mg CnCO,tL) (mg CnCOJ/L) (ml) ml ml ml I N5 s;, '-ten '-to So li+."1 2..1.'+ "'.'5 'Z,o...-:i \\60 Snmple hnrdncss (B) Measured spike value (MV) % R = MY I SY x JOO (mg CnCO.i/L) MY=A - B (ncccpl:tblc rnngc (mg CnC03/L) =75to125%) 'lo J-fC 100.0°/o S I amp e mens1treme11ts: Sample volume Begin End Totnl Hnrdncss Sample number Sample ID (ml) ml ml ml Multiplier (mg CnCO.i/Ll TV=Mn Blank -61Xt1rt-(SllOlllO OC V Oil! 1...aLU,;ILJ l\\0'5.~.~~ w-~~ t.. ~ ".>"....,""*or. ~"i 2..l '4-25.4. 1-1-.o -z_o.o '60 \\\\~~OS l.b j 3 ....L 1~.i+ ,2o,Q >-h1a q~ \\"'"\\~en.. e>--4 llr.n ~,, \\ C::..""1 3o.o ,,.,.:2. '} :.:t Ut_ 'i ().S.C.'\\. () \\ 2 I '2.?.1 '1.C I 2.1! ~'o 11oso1o.o\\ J 3 J_ -~.)

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I I ~~ C$Q/*<-J-Date reviewed I O.S*OCo"l'1 Note: If >151111 of titront is used, sample must be dilulcd. Reviewed by: Page 66of94 SOP C7 - Exhibit C7. I, revision 01*01-13 l l l 1 l l l l l J J J J ~ r~£ %d Bf;f&l tf?/01 ,:. )*... ,~.,, I 1 *J. -* L::...J ~ C..-."./ '\\------- ..... : Envilonnnnt.al Tc.it Ing SolullonJ. Inc. Analyst I \\.U Date analyzed l)?.01-* \\1-Hardness (SM 2340 C-1997) RL = I.0 mg CaC03/L g Page __ _ Page_~~of J Time initiated I iz:J02 Time completed :j}(j:o=:o:~e

...-_-_-_-_-_-~~~

Titrnut uormalit aml 11111/ti lier deter111i1111tio11: Titrnnt Normality check Begin End reference standard ml ml number number Lnborntor co11trol stmul11rtl: Reference stnndnrd True value Sample number (TV) volume (mg CnC03/L) (ml) Total ml (E) Begin ml End ml Normality (N) ofEDTA =0.2/E (ncccptnblc r:mgc = 0.0180 - 0.0220) o.owo pH Factor or Multiplier = (N x 50000)/ 50 ml sample =Nx 1000 Hnrdness (MY) % RS= MY /TV x 100 Total Multiplier (mg CnCOJIL) (ncccptnblc rnngc ml = 90 to 110%) (~ 40 50 ~-S 2\\.<; 2..0 zoo 40 HXHI. D /" I up /C(lfe samp e precmo11: ~ Snmple Hnrdncss %RPO"" Sample ID volume Begin End Total Multiplier (mg CnCOJIL) {(S - D) /l(S+0)/21) x 100 number (ml) ml ml ml ~ s Duplicate (B) ~ D ~ Matrix spike recovery: ~ Reference stnndard Spike value Snmple ~ Spike hnrdness (A) number (SY) volume: Begin ~ Totnl Multiplier (mg CnCO.i/L) (mg C:iCOJIL) (ml) ml ml ~ Snmple hnrdness (B) Measured spike value~ % R = MY I SY x I 00 (m1t CnCQ3/L) MY=A - B (ncccplnblc rnngc (mg C~CO..i!Ll ~ = 75 10 125%) ~ .. oan-1~ s I amp. e meosuremeuts: Snmple volume Begin End Tot:il H:irdness Snmple number Snmple ID (ml) ml ml ml Multiolier (mg CnCOJ/L) TV=ND Blank {should be= 0 mg CaCO;/Ll 8J C,.<; C\\.s o.o w :o tvo noc; o\\. ~ So.~ lO\\ f 50

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Pimephales promelas Environmental Tes.unq so1uuon1, Inc. Chronic Reference Toxicant Control Chart Source: In-house Culture 1.2 1.0 0.8 0.6 Control Limits(+/- 2 Standard Deviations) ~ 0.4 b.O -in d 1.2 ta "'C I " 1.0 Warning Limits ~.......................... 0.8 0.6 ~ ~ '............................ 0.4 Test date e 7-day 1Cz5 = 25% inhibition concentration. An estimation of the potassium chloride concentration which would cause a 25% reduction in Pimephales growth (calculated using ToxCalc). Central Tendency (mean logarithmic IC25 converted to anti-logarithmic values) Control Limits (mean logarithmic IC25 +/- 2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic IC25 +/- 2 coefficent of variations converted to anti-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +/- SA_75 converted to anti-logarithmic values, sA.75 = 751h percentile of CVs reported nationally by USE PA) Page 69of94 ~ lQ CD (nlffto~nMIT.,tt~ Soknlotn. ll'K.. c Pimephales promelas Chronic Reference Toxicant Control Chart Source: In-house Culture ~ Log10 Conversion Anti-logarithmic Values (g/L KCI) co T4',t number Test date 7-day IC25 Laboratory Calculated CV ToxCal Determination 7-day IC25 CT s CT Control Limits Warning Limits (g/L KCI) CT - ZS CT + ZS CT - ZCV CT + ZCV 1 04-04-16 0.7240 -0.1402 -0.1647 0.0544 0.6844 0.5328 0.8791 0.4629 0.9689 2 04-05-16 0.7762 -0.1100 -0.1569 0.0538 0.6968 0.5440 0.8925 0.4775 0.9777 3 05-10-16 0.7510 -0.1244 -0.1528 0.0511 0.7034 0.5559 0.8899 0.4937 0.9685 4 05-17-16 0.6409 -0.1932

• 0.15 73 0.0496 0.6961 0.5539 0.8749 0.4918 0.9530 5

06-07-16 0.6998 -0.1550 -0.1571 0.0468 0.6965 0.5614 0.8640 0.5026 0.9370 6 06-14-16 0.7412 0.1301 -0.1546 0.0451 0.7004 0.5690 0.8G23 0.5127 0.9315 7 07-12-16 0.7965

• 0.0988

-0.15*JO 0.0460 0.7080 0.5729 0.8749 0.5172 0.9437 8 07-19-16 0.6348 -0.1974 -0.1536 0.0459 0.7021 0.5682 0.8674 0.5114 0.9376 9 08-02-16 0.7850 -0.1051 -0.1502 0.0460 0.7077 0.5726 0.8746 0.5168 0.9436 10 08-09-16 0.6703 -0.1737 -0.1517 0.0447 0.7051 0.5739 0.8664 0.5190 0.9339 11 09-12-16 0.7241 -0.1402 -0.1510 0.0433 0.7063 0.5786 0.8622 0.5255 0.9270 12 09-12-16 0.7351 -0.1336 -0.1500 0.0421 0.7080 0.5831 0.8596 0.5315 0.9222 13 10-04-16 0.7791 -0.1084 -0.1477 0.0420 0.7117 0.5864 0.8638 0.5357 0.9254 14 11-01-16 0.7808 -0.1074 -0.1456 0.0419 0.7152 0.5897 0.8674 0.5398 0.9280 15 12-06-16 0.8303

• 0.0808

-0.1423 0.0433 0.7206 0.5904 0.8795 0.5399 0.9411 16 01-10-17 0.7066 -0.1508 -0.1433 0.0432 0.7189 0.5892 0.8773 0.5384 0.9392 17 02-07-17 0.7052 -0.1517 -0.142.1 0.0426 0.7209 0.5924 0.8772 0.5426 0.9378 18 03-07-17 0.7620 -0.1181 -0.1362 0.0365 0.7308 0.6178 0.8646 0.5761 0.9139 19 04-04-17 0.7043 -0.1523 -0.1392 0.0351 0.7258 0.6174 0.8531 0.5765 0.9012 20 OS-02-17 0.6740 -0.1714 -0.1371 0.0316 0.7292 0.6305 0.8434 0.5939 0.8858 Note: 7-day ICis = 25% inhibition concentrallon. An estimation of the potassium chloride concentration that would cause a 25% reduction in Plmepholes growth (calculated using ToxCalc). CT = Central tendency of the ICn values. S = Standard de~iation of the IC1s values. Control Limits= Mean logarithmic IC15 +/- 2 standard devlatlons converted to ant -logarithmic values. Warning Limits= Mean logarllhmlc ICu +/- 2CV ors. 75 converteCI to anti*logarlthmic values, S,.n = Standard deviation correspoMing to the l':i' percentile ol lVs reported nationally bv USEPA is.,. = 0.38). CV = Coe(flclenl of variation. 75th Percentile CV Warning Limits CT - SA.75 CT+ SA.7S 0.4243 0.9506 0.4320 0.9679 0.4361 0.9770 0.4316 0.9669 0.4318 0.9674 0.4343 0.9729 0.4389 0.9834 0.4353 0.9752 0.4388 0.9830 0.4372 0.9794 0.4379 0.9810 0.4389 0.9834 0.4413 0.9886 0.4434 0.9934 0.4468 1.0009 0.4457 0.9921 0.4469 0.9948 0.4531 1.0086 0.4500 1.0015 0.4521 1.0063 .s= ~ 0.... \\.!) 0... c: 0 u 0 0 c: .52...

• ~

ro > 0.... t: Cl.I u

E Cl.I 0

u 0 ~ Cl.I ro ro ro :-e c: tlO E - ..c - 3 0.... l!) 0....... c 0 u 0.... 1.25 1.00 0.75 a.so 0.25 20 10 0 30 20 10 0 Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture USEPA Minimum Acceptance Criteria (> 0.25 mg per surviving larvae) USEPA Maxi um Coefficient of Variation Guidance Criteria(< 20%) USEPA Upper PMSD Bound Acceptance Criteria(< 30%) USE PA Lower PMSD Bound ~~-------~------- ---------- - ------------ o.:...b 'f/>b 0,'\\.b 1.'\\.b "1.'\\.b o.-">b,_.">1:>,"J-">b r;,1.sb c"J*\\.b ">i.:'\\.b ">,,:...b r;,bsb cv">b cb'">b 1Y'°1 01 :...1 01:...1 &..,.1 0,_..">1 0o.!u 0o.*0 0<:/'~ r;:,s-"> r;:,b"' cb*'\\J 01-">- r;:,1 *,. r;:,'b' r;:,'b* r;:,"J' c"J* '\\.c- ..,_v ">1.: r;:,V "> r;:,1.* r;:,,, c°" 0c,* Test date Control Growth, Coefficient of Variation {CV) or Percent Minimum Significant Difference (PMSD) PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is nor a minimum acceptance criteria. Central Tendency (mean Control Growth. CV or PMSD) 95% Confidence Interval (mean Control Growth, CV or PMSD +/- 2 Standard Deviations) ~ Control Pimepha/es promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ToxCal Determination Control Growth Control Growth CV Control Growth Test (mg/uu1l1l larvHI !l<I Test number Test date Survival Mean CV MSD PMSD CT 95% Confidence Interval CT 95% Confidence Interval (%) (mll/lnlllal l*rvae) (%) (%) CT

• 2S CT+ 25 CT *25 CT+2S 1

04-04-16 100 0.461 5.9 0.0395 8.6 0.6125 0.415 0.810 5.3 0.0 10.6 ?. 04-05*16 100 0.647 12.3 0.1138 17.6 0.617 0.435 0.799 6.3

• 0.8 13.S 3

05-10-16 100 0.541 7.0 0.0614 11.3 0.608 0.431 0.785 6.4

• 0.2 13.1 4

05-17-16 100 0.507 1.8 0.0438 8.6 0.597 0.418 0.775 5.9 -1.0 12.9 5 06-07-16 100 0.421 5.0 0.0592 14.1 0.579 0.377 0.781 5.8 -0.7 12.4 6 06-14-16 100 0.455 3.2 0.0334 7.3 0.568 0.362 0.773 5.6 -0.B 12.0 7 07-12-16 100 0.578 8.8 0.0763 13.2 0.569 0.373 0.765 5.9 -0.5 12.3 8 07-19-16 100 0.774 10.1 0.1053 13.6 0.584 0.365 0.804 6.2 -0.4 12.7 9 08-02-16 100 0.570 3.4 0.0474 8.3 0.583 0372 0.794 6.0 -0.S 12.S 10 08-09-16 100 0.699 3.5 0.0827 11.8 0.591 0.379 0.803 5.8 -0.6 12.2 11 09-12-16 100 0.604 S.1 0.0532 8.8 0.592 0387 0.797 5.8 -0.4 ll.9 12 09-12-16 100 0.637 4.8 0.0520 8.2 0.595 0395 0.794 5.7 -0.3 11.7 13 10-04-16 100 0.588 3.2 0.0506 8.6 0.594 0.401 0.788 5.6

• 0.4 11.5 14 11-01-16 100 0.671 7.7 0.0627 9.3 0.598 0.A07 0.790 5.7

-0.2 11.S lS 12-06-16 100 0.617 S.6 0.1167 18.9 0.599 0.413 0.786 5.7 o.o 11.4 16 01-10*17 100 0.587 7.3 0.0629 10.7 0.600 0.414 0.786 5.7 0.0 11.3 17 02*07-17 100 0.579 11.5 0.0751 13.0 0.591 0.420 0.763 6.2 0.3 12.0 18 03-07-17 100 0.638 6.2 0.0662 10.4 0.590 0.421 0.759 6.2 0.4 12.0 19 04-04-17 100 0.620 5.5 0.0919 14.8 0.591 0.<122 0.761 6.3 0.6 11.q 20 05-02-17 100 0.729 5.6 0.0792 10.9 0.596 0.416 0.776 6.2 0.6 11.8 Nore; Control Survlvaf = USEPA min mum te.n u'eotabUtty c11teria l. 80".4 s1.1rv1val. "'ontrol Mean Growth:;:;: USEPA mloimutfl rest cc.ee1na~fii 1 y cntena ~ 0.25 m1/surv1v ng lilNU*. OJ ~ Cof!ffte~m or \\'irtiJ:dor for contro11rowth. USEPA miJ:dmum (\\/ g ukf~lltt c,1t~ria (901 "' percenlite, < 10% MSD

• Minimum >i&nlftcont d fleronce.

PMSO Pcrctnt minimum Stgn1f1nttt difference. PMSO,,..a measUt of ~e n precision. The PMSO ts 1'1e m1n11nurn percent difte,tmce betw,en the control and lrHrmec,t that can be: declarfd stau~Outly ngniflcitrn In 1 whote elftucmt to'l!IClty tMt. Lower PMSD bound determined by USEPA (10'" percon11le) > 12%. 'The bw~' PMSO bound represents.i pr.cticaf ttmir to the senmlVltv of tM test rMttiod,;,nd IJ not 1 min.mum 1<<epu1nce a 1tena. Upper PMSD boond *a:eptonco criteria dotermlned bv USEPA (90" percen1llel < 30%. ct'=: CPntr."I riantf*f\\(\\I ot th! l'OWlh, CV or PMSOv~lu". s~ Standard devi1tl0rlot the growth, C\\/ o' PMSOv lues. CT 10.7 11.7 11.7 11.3 11.6 11.2 11.4 11.5 11.3 11.4 11.2 11.0 10.9 10.8 11.2 11.2 11.4 11.2 11.4 Test PM50 (%) 95% Confidence Interval CT

• 25 5.8 4.8 5.3 5.0 5.4 4.8 5.2 5.5 5.2 5.5 5.4 5.2 5.1 5.2 4.6 4.6 4.8 4.7 4 7 5.0 CT+25 15.7 18.6 18.0 17.6 17.8 17.6 17.6 17.6 17.4 17.2 17.0 16.8 16.6 16.4 17.8 17.7 17.9 17.7 17 9 17.8 11'!--

~ 0 r-:-::-:i r--:-i,,,.-...-.

• I

'I-'-' - -

• I *--

1 I tnYironrMnulTf'stlng Sc.lo1'°"s. l1K. Page I of6 Potassium Chloride Chronic Reference Toxicant Test (EPA-822-R-02-013 Method 1000.0) Species: Pimeplzales prome/as Dilution re arntion infor111atio11: KCI Stock lNSS number: lNSS \\ S&i.o Stock preparation: Dilution prep (mg/L) 300 450 Stock volume (mL) 9 13.5 Diluent volume (mL) 1491 1486.5 Totnl volume (mL) 1500 1500 Test oruan;sm informatio11: Onranism source: Age: Spawn date: Hatch dates and times: Transfer vessel information: Average transfer volume: 50 g KCl/L: Dissolve 50 o KCI in 1-L Milli-water. 600 750 900 18 22.5 27 1482 1477.5 1473 1:500 1500 1500 In-house culture < 24-hours old <:>'>*Ci\\*\\"'\\ \\""\\OQ ~ o<:..-o-i.-n ~t.ooS 1050 31.5 1468.5 1500 pH= \\.21.. S.U. Temperature = "l..~.C\\ °C < 0.25 mL Daily feeding aud renewal information: D:iy D:itc Morning feeding Afternoon feeding Time Analyst Time Analyst 0 05-02-17 ()o\\0 X{ \\'1.~o u I 05-03-17 c~ ,.{ \\~c.c 2 05-04-17 D\\oO C,, ~1 n... o~ \\,I. \\1 3 ~l u 05-05-17 Ob\\C \\"l.l b l( 4 05-06-17 ~l C"l\\ c; \\~\\<,. 5 05-07-17 0"#\\0 't\\ \\ "!l'\\C H 6 05-0R-17 Olo \\<':, }t \\'1.\\.<.> i\\\\ 7 v 05-09-17 PpKCICR Test Number: 25 Comments: Test iuformntion: Randomizino tem late: Incubator number and shelf location: Artemia CHM number: CHM914 Drying information for weight determi11atio11: Date I Time in oven: Initial oven tern erature: lDa*c_ Date I Time out of oven: O'MO*"('\\ 0 Final oven tern erature: Total drying time: Test initiation, renewal, l\\fl-ISW or termin:ition batch usccJ Time An:ilyst o~ "1..0 ~ o'-l*u.*11 A 09~'-\\ 0'\\* 11o.,, ~ o<;;.~c=\\ ~ o+t.b*f'\\ & 0&'°'40 \\\\ o'-l

• i.~. ', "'

ol\\\\5 i\\ O~*Cfl..*\\1 O'\\t..\\O At OS*Ol.*'1 o'\\01-Ai CS*<n..,, o i "to ~\\ - Control illformalion: Acceptnnce criteria 0. $ 20% Average weight per surviving larvae: 2: 0.25 mg/larvae Page 73 of94 SOP AT21 - Exhibit AT2 I. I, revision l 1*01 -14 '"i i ~ r? ~ (;::i

I --

I. *....__ Page 2 of6 I _.../ ) EnvlronrTtt.nt~I Tc~Ung SoI11Uans.lr.c.. Species: Pimeplzales promelas PpKCICR Test Number: 25 Survival and Growth Data Day Control 300 mg KCl/L 450 mg KCl/L A B c D E F G H I J K L 0 10 10 10 10 10 10 10 10 10 10 10 10 l / D <o I O to 10 IO 10 Jo 10 10 IQ 1a 2 t~ lQ to (O {Q IO IQ \\C) LC) lo IO 10 3 I\\) (Q lb I() IC) 10 (() 10 I\\) I {J IC 10 4 10 10 /0 JO 'O IQ /0 IO ID I() IQ IQ 5 I 'O It) ID IQ LD { () IQ ( () co IQ LO 10 6 JU m f{) (0 lO 10 I () 10 IQ l() lO 10 7 ft) I() I D 10 ro to lb 10 ID ( () 10 ., 0 A = Pan weight (mg) Tray color code:: U:g h I-BL".e. 15.IS 15.~ j\\,,}.S lb.'18 15.'°40 l3.~2 1-:iOO IS.e=f IS.3s llo.18 \\4.~z. ls. °37) Annlyst: 'i-- Date: OY* Z:Z-1~ 13 = ran + Larvnc weight (mg) ~~.le J.~.'-ll ?13.88 ~,,a, ltt.~4 ~ *. oe ~.16 ia1.q~ 22.cH io.'¥1 ~o.1~ Analyst: lA...J citl.'5l> Date: os.11.n-C = Larvae wc.ight (mg)= B - A Hand cnlculntcd. h.Co~,,olo 1. 1..~ ". &?>> <..to S,t\\"L '-* () 2,.'?..'\\ Co.SC\\ s. C. c.. a1 SA"\\ Analyst: ~ Weight per inilinl number of larvae (mg) =C f Initial number of lnrvne fl.,,., oi ~ "'6'.. ~ 6' }- It') ~ ~ ..,0 (J..'V Hnnd cnlcul:itcd. £ t.; \\0 ~ t '1) '? \\!) A1rnlysl: o* 'V. C) ' 'V' 'I;) o* t$ (). ~ * (). '()" o* Average weight per inilial Pc recur number of J:ir"ae (mg) red uction

r.

ef.* o. b~S \\ '2..'\\ 1.

o. ~a.Ci n. & <.

0.11..'\\ l~-t~~' -Ll~.. 0 f from conlrol ("/o)

• ~*

Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk =sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: Page 74of94 SOPAT21 - Exhibit AT21.J, revision I J-01-14 Page 3 of6 EIWfronmen1aJ Teilin9 SGlutJOns., lnc. Species: Phnep!tales promelas PpKCICR Test Number: 25 Survival and Growth Data Day 600 mg KCl/L 750 m17 KCl/L 900 mp KCl/L M N 0 p Q

• R s

T u v w x 0 10 !O 10 IO 10 JO JO 10 10 10 10 10 J 10 ID 10 ( () co IO "11{ 10 '\\ t.( IQ I{) I l) 2 10 10 10 tO 10 r o ro '() 10 °" '*~ ~ 3 10 ID ID l () ID t() J 0 I() 10 ~ '\\ °' 4 \\~ l#. "l-'.

• ~ '"'

(0 LO 'D io l \\.) " ~ K '\\ .f 5 '6u.. 10 ~~ IQ ~ &~ \\ ~ f ).\\ "'° ~ ~ 6 g 10 " 10 \\ -,'A l ~, ~ ~ lo 7 ~ \\ () ~ H) \\ .,,..( t \\ \\. \\.. A = Pan weight (mg) '1.u 1c;,qz. Tr:ty color code: : :=.h:qM

e.

l'i6Lt IS.e;.t l"i.llv IS.l.I.{ ll0.12, I "~:t IS.03 15.£:\\~ is.~o IS.le lto.6~ Analyst: ~ Date: o~. a.";}. ~r"l B.. Pan + Larvae weight (mg) Do.41 l.t.ll. ~o.oe 2o.£fz. a..o.~ \\'t1S IS.&=\\ 2o.11 Analyst: ~ a~.3l ~1.~8 l'f °' 20.2e Dale: o:;_ u.1-:r C = L:irvac weig ht (mg)= B - /\\ Hand calculated. S.ii 1...4'; i.. c'-\\ ".iii. 'i.8'1 q.to t.(.U s.t~ ~.10 4.~~ ~. \\.\\ :>.1.-1 Analyst: J 'Weight per initial numllcr of larvae (mg) = CI Initial number ofl:1rvnc ~ N <,,~ "'o ~,,,, t\\/ ~ $' ~ ~ v,O ~"' ~.;j Hand cnlculnted. ~ ~ 0 v .)..: .).. ~ '? Analyst: t)' o* ~r o* o* 0 ~* tr o* o* c;)' A\\'erngc weight per initial Percent number of larvae (mg) reductio11 from control o.1o'2.& r~. ~ 1. 0. 1.\\1~ ~"\\.~1.

o. ~'15

~s. C\\ 1. (% ) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comme11ts: Page 75 of94 SOP AT2 l - Exhibit AT2 l.1, revision l 1-01-14 0 I I L-::J r;::J 0 Species: Pimephales promelas PpKCICR Test Number: 25 Survival mu/ Growth Dain Day 1050 mg KCl/L y z AA BB 0 10 JO JO 10 l ,~,~ t. "\\...\\ lo '4~ 2 l...... !Q'" (g to 3 '-\\.,,.,. \\D \\, s 4 v.... w.. "'.I. "'L ~ ~ L-5 '\\A

• ~

l t "'Z... \\ 6 '" '" c"' \\ () \\ 7 \\ t:> \\ 0 A = Pnn weight (m, .ci. ,..,...,.. 'i.. ~*"' Tray color code:: l~l+ &sve., l110'i ~ 11o.l8 ~ Analyst: ~ Daic: CH. ~~-n B = Pan+ Lan*ae 11*eigb1 (mg) 11.ot 7 t~'lll ~~ n:1ly t: Dntc:

05. }l.j'.}::

6t C "' Larvae weigh I (mg)= n - A o."10 Hnn tl cnlculatcd. ~\\ 0 ;1'&) ~ i."' ~ Analyst: I>.. Weighl per initial nu mber ofl:tr\\'ne (mg) "' CI Initial number of lnr\\'nc ~,..,, 0 \\..' 0 Hnnd enlculatcd. ~ Analyst: ()' ()' Avcrnge weight per inilial l'crecnt number oflnrvnc (mg) rcducrion o. o~t:\\ ~'{.11. from control (%) Comment codes: c =clear, d = dead, fg =fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg = unusually large d&r = decanted and returned, w,,.. wounded. Comme11ts: Page4 of6 Page 76 of94 SOP A TI 1 - Ex11ibit A T21. I, revision 11 14 ,_.,,.,.11o"'"'"l""' ConUol JOO 450 600 750 9UU 10$0 >unnctrs MSO 'lilluei 'NISD: "*.Mtel* A 8 c D E F G H I I K l M N 0 p 0 R s T u v w x y z AA 88 111111*!......m\\*tDI rl".tm.mb.,ot ,...,_.. tttit(,.-icJ 10 10 IS.IS 10 10 lS.3S 10 10 !6.2S 10 10 ls.98 10 10 15.40 JO 10 13.42 10 10 15.00 10 10 15.1!9 10 10 15.35 10 10 1618 JO I 10 14~7:? 10 10 1530 10 8 14.54 10 10 15.92 10 9 151"' 10 10 14.96 10 7 lS.2* 10 7 16.12 10 7 14.97 10 g 15.()3 10 7 15.95 10 6 IMO 10 6 15.78 10 6 16.57 10 I 16.** 10 0 0.00 10 I 16 IS 10 0 0.00 0.0792 M$D : 10.9 PMSO : Plmepho/es promelos Chronic Reference ToKicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Veri' ication of Data Entry, Calculations, nd Statistical Analyses I

• Patt* l*fn*

a...,..,,,....,.,, (rnd

• Waltftt/S**vt,...*1 M.... wt l~/ Swvtwf*&

C'at md* "lofvwlaciori W*l &h1/~1lljn~*** wefcht(md nwnbu of hrv** Imel 11umtt.1 ol tr"'n lm1l ~..... l~(lftfl -**1ooo*.... 1tKI 22.78 7.63 0 76? 0.763 22.41 7.06 0.706 0.129 5.6 0.706 23 88 7.63 0,763 0.763 22.81 6.83 0.683 0,683 2150 6J.Q 0.6~C!,__ 0.6 10 19.34 S.9' 0 592 O.E35 10.0 0.592 21 08 6 08 o.608 0.608 23.l& 7.Z9 0.729 0.729 H.94 6.59 0.659 0.659 12.04 S.86 0586 O.!i99 80 0.5!6 2079 6.07 0.607 0.607 20.74 5.44 0.544 0.54* 2041 5.87 0.734 0.587 22.37 6.45 0.645 081 S.5 000 ll.BS 6.04 0.671 0.60-I 21.n 6.76 0.676 0,676 20.08 4.84 0.691 0.48.> 20.92 4.80 0.686 0659 6.1 o.~ 19.19 4.22 0.603 0412 10.28 5.Z5 0.656 O.S15 20.65 4,70 0.671 O.A70 19.7S ,,n 0.725 14.l 0.4J5 0.630 18 89 3.11 0.518 0.311 20.19 3.6? 0.603 0362 17.07 o.n 0.730 0.073 0.00 0.00 0.000 0.:10 7.l 0000 1699 0.81 0.810 0081 0.00 0.00 0000 0.000 MtlWl'tum SlcnFic.lnt Olffe,..,,CC! P-~rcont Minimum S*snlfte*nt Difference TOll number: 2S ~-----------~ T*tt dates: ________ M_*v_o_2_-0_9_._20_1_1 M.. 1't1H¥1"~(l') ~~aJ'i w.fit\\l/ ~ftut Cocffhflf'll Of Wot tUOI\\ r.u'" r*dl.kUon ""' ttul'l\\beroJ l11rvH 11 (Gft!Utf '"'* tml) 100.0 0.729 5.6 Not applicable 100.0 0.635 10.0 12.9 100.0 O.S99 1.0 17.8 92.5 0.628 6.4 13.8 71.S o.*18 8.9 34.4 62.. S 0.395 18.1 4S.9 s.o 0.039 US.8 94.7 PMSO ti a rnHuirt of tut i:iredsico Tht PM.SO b ~ he minimum otr(ent cllfferenct: t>etwnn the c°"trot 1nd trutrnenl that ati be decf.-rcd il1ttistieallv s ift n ifk~t1I In a wttole effluent tow1dty test Lower PMSO bound cleterMined brt USEPA ( 101h PfHC'tntlle) :s 1 l t-4 Up~r PMSO bound det*rmlncd by USEPA (90th perc:enllle).;;: 50'4. lower and pp.er PMSO boufld\\ wen detcrminud from the 10th 1nd 90th pernntile, P.tt1 ety, of PMSO d:.lt;i from ( PA's WET ll'tterbbOr.Horv Varl~bllilV' Slud°' ~USEPA, 2001~ USEP". 2001b~ The fowe1 PMSJ botmd ropunef'IU

• PrKllul llm{l lo th~ iansitwlh of the test method iltid is not a minimum ~:epunc:e: critcrii tJS!PA 20011, 2001D. Flnal Reoon: 1tut1rl1tDOnfOl"J vat1abU11y !:t.hJov of U'o\\ Short-te.rm Chtonie at\\d Acute Whole Eltl\\Jent To*icltyTest Methoch, V~umos 1 tl'ld l*Appctndl'(. EPA*ll:J..S..01..()04 :and EPA.-321-1*0 1..00S. us Envlfor\\mental Prote<tion Agencv. Cln i:lnn~t*, OH.

St.art oa e-51212011 End Date: 51912017 Sample Date: Comments: Conc-m IL D*ContrOI 300 450 eoo 750 '900 1050 Conc-m9/L D-Control 300 450 600 750 "900 "1050 Auxllia!l'. Tests 1.0000 1.0000 1.0000 0 8000 0.7000 0,7000 0.1000 Mean 1.0000 1.0000 1.0000 0.9250 0.7250 06250 0.0500 2 1.0000 1 0000 1 0000 1.0000 07000 0.6000 0.0000 N-M11an 1 0000 1.0000 1.0000 0.9250 0.7250 0 6250 0.0500 Sta tistical Analyses Larval Fish Growlh and Survival Test-7 OaySu.rvlval Test ID: PpKClCR S;j111µ1~ ID. REt>- RefTOAi1,;Cill\\ Lab ID* ETS-Envir. Testing Sol. Sample T'fpe: KCL-Potassium c:hlof!de Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas J 4 1.0000 1 0000 1.0000 1.0000 1.0000 1.0000 0.9000 1 0000 0.7000 0.8000 0.6000 0.6000 0.1000 0.0000 Transform : Arcsln S9uarc Root Rank 1-Talled Number Mean Min Max CV% N S11m Crltlr.:ll Rasp 1.4120 1.4120 1.4120 0.000 4 0 1.4120 1.4120 1.4120 0000 4

• a.oo 10 00 0

1A120 1.4120 1.4120 0.000 4 " 8.oo 10 00 0 1 2951 1 1071 1.4120 11 347 4 '4.00 10.00 3 1.0202 0.9912 1.1071 5.685 4 ~ 0. 00 10 00 11 0 9123 0.8861 0.9912 5.759 4 10.00 10 00 15 0.2403 0.1588 0,3218 39.161 4

• o.oo 10.00 38 Statistic Critical Skew Shapiro-Will<'s Tasl indicates non-normal distribution (p <= 0.01) 0.87827 0 896

-0.3Be8 Eguaht~ o* vanance cannot be connrrned H~Eot hesis Test (1-tan, 0.05! NOEC Steel's Many-One Rank Test 600 Treatments vs 0-Control Trim Level EC50 95"/. CL 0.0% 5.0% 10.0% 876 51 S34 62 920.49 20.0o/o 897.43 84837 949.33 Aul°'S.0% 865.76 826.27 907.13 09 08 .. 07 ~ 0.6 "'0.5 Cl 0 4 r- 03 0.2 0.1 0 g g c 0 ll 0 Page 78of94 LOEC 750 c.nv TU 670.82 Trlmmed Spearman-Karber 10..-~~~~~~~~~~~-, 09 08 07

l 0.6 c

&. 0 5 ~ Od 03 02 0 I Dose-Response Plot 10 100 1000 10000 Dose mglL Total Number 40 40 40 40 40 40 40 Kurt 1 96258 Stan Dale : 5/212017 End Oa1;i* 5/9/2017 Sample Date Comments: Co nc-m IL 0-Control 300 450 600 750 900 1050 0.7530 0.6100 0.6590 0.5870 0.4840 0.4700 0.0730 2 0.7060 0.5920 0.5860 0 6450 0-4800 0.4350 0.0000 Statistical Analyses Larv:JI Fish Growth an d Survival Tcst-7 Day Growth Te~ I ID* Ppl(CtCR S;omplA tn* RFl'°-RAr Tn~ic:;,nt Lab I~ ETS-Envir. Testing Sot Sample Typ. KCL-Potasslum chloride Pro1Dco1 FWCHR-EPA-821-R-02-013 Test Spec1 s PP-Pimephales promelas 3 4 07630 0.6830 0.6090 0.7290 0.6070 0.54'50 0 6040 0.6760 04220

0. 5250 0.3110 0.3620 0 0810 0 0000 Transform: Untransformed 1-Tafled Isotonic:

Conc*m!l'L Mean N-Mean Moan tJlin Max CV% N t-Stat Critical MSD 0 -Control 0.7288 1.0000 0.7288 0.6830 0 7630 5 578 4

• 300 0.6348 0.8710 0.6348 0.5920 0.7290 9.980 4

2.718 2.290 0,0792

• 450 0.5990 0 8220 0 5990 0.5440 0.6590 7.982 4

3.752 2.290 0.0792

• soc 0.6280 0.8617 0.6280 0.5870 0 6760 6.403 4

2 913 2.290 0.0792 750 0.4778 0.655 0.4778 0.4220 0.5250 6.868 4 900 0.3945 0.5413 0.3945 0.3110 0.4700 18.143 4 1050 0.0385 0.0528 0.0385 0.0000 0.0810 115.781 4 Auxillal"t'. Tests Statistic Critical Shapiro-Wilk's Teslindicates normal distribution (p > 0.01) 0.92374 0.844 Bartlett's Test indicate$ egual variances(!!= 0.66) 0.76291 11.3449 H~ poth esis Test (1*Uil, 0.05) NOEC LOEC ChV TU MS Du MSDp MSB MSE Ounnett's Test <300 00 0.07919 0.10867 0.01266 0.00239 Treatments vs D-Control Lin crar lntcrpolotlon (ZOO Rc::;amplc:;) Point mg IL SD 1co5* 116 29 61.66 1c10* 232.58 85.50 IC15 408.09 138.74 IC20 633.70 58.72 IC25 673.96 19.94 IC40 822.97 41.45 ICSO 912.69 18.66 95% CL(Ex p ) Skew 54.14 436.34 1 8870 108.28 6D2.01 1.6404 126.89 754.60 0 3100 316.33 686.56 -3.8530 613.55 747.38 0.2701 715 37 958.28 0 3965 829 43 945.96

• 1 0693 0.9 0.8 07
• indicates IC estimate le$$ than the lowest conc.entration

=: 0.6 0.9 0.8 07 i 0.6 e o.5 Cl

04 0

... 0.3 01 0 I 0 Page 79 of94 e c 0 0 6 c: g_ 0.5 ~ 04 OJ 0.2 01 Dose-Response Plot 500 1000 Dose mg/L 1 -!all, 0.05 level of s gnlllcance Mean N-lllean 0.7288 l.0000 0.6348 0.8710 0.6135 0.8419 0.6135 08419 0 4778 06556 0.3945 0 5413 0.0385 0 0528 Sk.ew Kurt 0.70843 -0.4102 F-Prob d f 0 0148 3 12 1500 ~l

• .-t-i ~ P Page 5 of6 1

Species: Pimeplzales promelas PpKCICR Test Number: 25 Daily C!temist1y: Day (Analvsl identified for cnch day, pcrfom1ed pH, D.O. and conductivity measurements onlv.) 0 I 2 Analyst 1l1 t-IL- ~ Conccntmtion Parameter ~- Q *.. -.

• ~

-:~

~

l.

- ~-*~" pH(S.U.) g,2.\\ ".}Pt~ e>.10 "t.':\\Z. e.oe -+:~3 DO (mg/L) 't.!1 '.J..to +..:r /l.. Y

• +.~

'~.2.. Conducfi\\'ity (11111hos/cm) 32..\\ -32.6 '3 t<i' I* C ONTRO L "Alkalinity r..,: *;;: ~-~ - .._~I,.. ~\\ ~-:~ <v '4~ (m~ CaCOJIL) -* ~ " H:irdnc s - '.'[,,. ~ i ~ 8~ ~'?.. (mg CaC01/L)

• Temperature (0C)

,_'\\."'\\ "\\S. '1. ~., ,.,1..\\. \\ 1."'\\.l.o "'\\.&.\\. ~ pH (S.U.) 0.'1* ".l.e>j ~.n"l-1-\\40 So,p '\\.-s:1 DO (mg/L) ~-i:\\ f).._\\O "+.'l ~. \\.( 1-..d ".\\A JOO mg KCl/L Cnn<l nrtivity l ~ ~ ~2 eq0 ett \\ (um hos/cm) . (

• Tem perature (0C)

"1.'\\.i... 'L..S."\\ -u>.,.\\ "l.'-\\*S "1..o.\\.~ \\.."\\*'\\ pH (S.U.} e.\\'5 -1..~ &O<P ~.\\12 0()5 '.} *"' l DO (mg/L) '}.'\\ 'l.ta "".}.~ '+.3 /l.A ~.'?i 450 mg KCl/L Conducli"itY i°'\\O 10"10 (µmhos/cm) ~

• ~

\\070

• i
• ..r_...!

...:~ " Tcmpernture (°C) "l.'\\.<o "'l-..o "'\\... \\.a '\\_'-\\. ~ L."'\\.lo "'\\)\\.,_ pH (S.U.) 0.\\S 1._~.:;- e,.05 ~.t,O, AD~ ~. \\I l DO (mg/L) e.o '.}lb 1\\-a ~.t, "\\!1 -:\\..s 600 mg KCl/L Conductivity 13'10 low

• l320 0

(~1mhus/c111)

• Temperature (°C)

(.~lo '1..."'.* (- "\\.'\\-& L."\\*5 t.'-\\.1 'bl.,_ p H (S.U.} 'O.\\~ 1-.e4 e.o5 ~.1~n e.02 '+.\\10 DO (mg/L) eo 'l-.~ 8.o "\\;3 Q.Q ~3 750 mg KCl/L Cond11c1ivity 161o (~tmhos/cm) lSLIO 16&.J "'Tcmpernture (0C) 1,"1,.. lc. 'Lt;.o "'\\.~ . J i."\\* 1 \\.U.-S pH (5.U.) e.\\~ 't.02. ~.o'1 1-.l61. e.03 '+.5t1 DO (mg/L) Q.0 ~."l-t3J:J /l.3 ~.o .}.3 900 mg KCUL Contl11c1i\\*ity 1.,. le>\\O 1e3o le20 ~ (~tmhos/cm) ..:;.:.~*.,.. "Temperntur e (0C) 1..L\\. lo 1..~.o "l... \\. ~ -i'-\\.'4 "'\\."'\\. ~ ,,~.s pH (S.U.} g.,,?> ').C'~ 0.o~ 'l ti~ e&+ '+.Sf-DO (mg/L) A.I +.u ~.o 1-.~ 6.o "'.}. ~ 1050 mg KCl/L Conducli\\'il)' ';"(... 1l ~1:50 ~100 ~\\20 (µrnhos/cm} ~

• Temperature (°C) 1.1.\\."1

"\\...*-\\.'\\ '"l.-!-'\\ 1..1.\\ *.., 1.c.\\. ~ 1."\\. lo Initial Final Initial Final Initial Final

• Temperatures performed :ll the time oftest initiation, renewal or termination by the analyst identified in the Doily Rcnew~l lnfonnnlion table loc.-ited on Page I.
• nlinity nnd hardnCEs performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: t.

ltn w iw \\ Page 80of94 SOP AT21 - Exhibit AT21.I, revision 11-01-14 . I I 0 r _,_] [_ -l r.Q o I i l r* I 1-: Page 6 of6 Species: Pimeplzales promelas PpKCICR Test Number: 25 Day {Analyst identified for each day. performed pH, 0.0. and conductivity measurements only.) 3 4 5 6 Analyst ~ Pf:..t,...... (<.J IA-l.l.__ v--- l)V jJ ,)v Concentration Parameter ~

• *~

IP 1 ~ 7' .r., r* *. I ~-

• ~

pH (S.U.) Pu" -:?.Cf~ ?,.oi ').~ "'} 0.\\'5 "t.e2 e.~ 'J.w DO (mg/L) "":l~ ~- 1 -i. q ~q ':\\.:} ':l-lP ":).,A ~. o Conductivity ~20

. - -~

bZ.U 311-32'-{ (lam hos/cm) -r.';r_ __......,.*.~.*~ ~ -.:J -

• Alkalinity

~ !~"' CONTROL

~f'-

~~ . ~ -. *;~ (mg CaCO,!L) ~ ~--- ...... ~ --- _.

• Hardness '

.~;;: v - ~ g(o (m!!. CaCOJIL)

• Temperature

""l!\\.\\o "\\...\\..-.\\ "1.A. <a '""\\. "\\- lo ~. f "\\... ~-"\\ !>\\. & t.-l.* ~ (oC) pH (S.U.) M1-1.'15 ~ - \\! ')..53 0.\\0 ~-1'll ~,r:;: ')-,\\.( ~ DO (mgfL) ~ ~ ?-.'1 J *<6 '\\.0 .-:\\..~ I'}.~ /l..tl

14. \\

Conductivity 300 mg KCl/L <0'4 \\ '6'tt4 0&1 0I05 (;tmhos/cm)

• • ~ -
• Temperature

\\."\\... r "\\.4.\\a C..'l* 1 '\\."\\* ~ "\\..'-'*' '"1.'\\.\\o "\\.-{. '\\ "\\.'\\-1... c*q pH(S.U.) 611v.1 1.":.11.1 <(. \\2- 'l, Q" ~.c:A 'llA~ 0..\\~ 'i-*62-DO (mgfL) ".\\~ +.r -:J A ~.o r:.\\-.i- 'l.ra "'.\\-.~ (t.3 Conductivity 450 mg I CllL l011) hC.O h'iO \\HO {µmhos/can)

• Temperature

""\\.'\\,.lo '\\.'\\.1 '""L'-\\* \\ l,.'{.\\ "'-"l*1 \\...'-\\*L "l!V\\ "1.'\\*).. c*ci pH (S.U.) 0-05 "J.'1-'l <6. \\ '2.- ~-~ 8.dl 'lM (JI ~. \\~ 'l...sY DO (mgfL) ~ 1"\\ f.q S..J (Oft l.l.~ ').ca ".). t\\ ~. 2> 600 mg KCl/L Condncli\\'ity \\320 i l3qo a'-( 2.0 {11mhos/cm) \\ l~O

• Tempernture L_-.\\. b 1.1\\ *~

'1..'-l-1 '"\\..'-\\* (.o l.~-1 L.'-\\..1.... L.c.\\.'\\ '1.'\\.-S ('C) pH (S.U.) e.os -:J.'1a '"fl.\\' ':l-.50 e.~ '+-v~ 6.,\\~ 'l,l./G\\ DO (mg/L) e,o -:)._" i.o ~.,o ' t-d ~ *5" 00 \\(1. 3 Conducti\\*ity ~ - 750 mg KCl/L lS9J \\~~ l~':lO (~2.0 (µmhos/cm) ~'~ "Temperature l.t.\\ ':"'\\ C.~*"\\ '"\\."l-1 ~-~ '"cl.1 t.."\\.S L."\\. '\\ )..'-\\.~ ("C) pH (S.U.) 0.o~ 1-'l l ~. I\\ ~-5!; e.oe /)..tJef 0.,\\2.. "\\....,~ DO (mg/L) 8,l

}.-:J..

4i *" I.DB q_(3 'l-5" 8.o ~. t Conductivity 900 mg KCl/L t?bQ l.-o\\D ~ f0a'}o h3~0 (µ111hus/c111)

• Tcmpernture

'l..'i* l ).'\\.'\\ t."\\. f '\\."\\."" "2..'\\-1 \\...'-\\* 1.. ~., l-4..~ (°C) pH (S.U.) e.o~ T--:µ; ~.'09 ~.vo A.o~ 'l..'1-'\\ 0.\\2. 11..UP> DO (mgfL) B.I +.-+- j,'2.- 4.~ ' ~. e;j r.}.u 'b.l l.O.?. Conduclivity 1050 mg KCl/L (1lmhos/cm) Z \\lcO 2-\\G:lf) Al'-f 0 ~'YO ~

• Temperature

'1.~. lo t't*~ £.~-& 1...4* ~ 1."\\. "'\\ 1.~. '{ 1~.c) 1.4*~ (°Cl Initial Final Initial Final Initial Final Initial Final .,,::::~\\: ' pcraturcs performed al Liie time oftest Initiation, renewal or 1erminmion by the urrnlyst idt:11ti1icd i11 the Daily Renewal Information table located on Page I. ~lka inily and hardness performed by the analyst identified on tile bench sheet specific for each analysis and transcribed to this bench sheet by: _ Page 81 of 94 SOP A T21 - Exhibit AT2 I. I, revision l l-01-14 -u nJ z _, 0.0 -"' N u nJ "O I " Ceriodaphnia dubia Envlronmcmt411 Tostlng Solutions,, Inc. Chronic Reference Toxicant Control Chart Source: In-house Culture 1.08 1.06 1.04 1.15 1.10 1.05 1.00 Control Limits (+/- 2 Standard Deviations) ~----~--------~------~------* o * '" *.. * '"

• o * "

o o <f I o

• o o *.. '" o o o
• o o o o., o o
• o "
• o o o *
• o o '
• ., '" o '

o o ' o o o '

• .._ o *
• o
• o
• o o *
• t t... o t....

t I o Warning Limits ..... *~* . ~ f . 4.,... Test date 7-day IC25 = 25% inhibition concentration. An estimation of the sodium chloride concentration which would cause a 25% reduction in Ceriodaphnia reproduction (calculated using ToxCalc). Central Tendency (mean logarithmic IC25 oonverted to anti-logarithmic values) Control Limits (mean logarithmic IC25 +/- 2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic IC25 +/- 2 coefficent of variations converted to anti-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +/- 5A.io converted to anti-logarithmic values, sA.io = 10th percentile of CVs reported nationally by USE PA) Page 82of94 ~ CQ (I) co* W f:1wo :mnwnc.at Ttt'""SI Sol1,111Df'l,-, Inc, 0 - (0 Test number Test date 1 01-12-16 2 02-09-16 3 02-09-16 4 03-08-16 5 04-05-16 6 04-05-16 7 05-10-16 8 06-07-16 9 07-12-16 10 08-02-16 11 09-13-16 12 10-04-16 13 11-01-16 14 12-06-16 15 01-10-17 16 02-07-17 17 03-07-17 18 03-07-17 19 04-04-17 20 05-02-17 7-day IC25 ToxCal Detem1lnatlon 7-day IC25 (g/l N<iCIJ 1.0562 0.0238 1.0581 0.0245 1.0624 0.0263 1.0690 0.0290 1.0720 0.0302 1.0627 0.0264 1.0605 0.0255 1.0538 0.0228 1.0579 0.0244 1.0662 0.0278 1.0628 0.0265 1.0768 0.0321 1.0785 0.0328 1.0753 0.0315 1.0723 0.0303 1.0641 0.0270 1.0641 0.0270 1.0614 0.0259 1.0654 0.0275 1.0691 0.0290 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Source: In-house Culture Log 10 Conversion Anti-logarit hmic Values (g/l NaCl) Laboratory Calculated CV CT s CT Control Limits Warning Limits CT

• 2S CT + 2S CT - 2CV CT + 2CV 0.0283 0.0031 1.0673 1.0520 1.0828 1.0530 1.0816 0.0281 0.0032 1.0668 1.0510 1.0829 1.0520 1.0816 0.0281 0.0032 1.0668 1.0510 1.0829 1.0520 1.0816 0.0281 0.0032 1.0668 1.0510 1.0828 1.0520 1.0815 0.0279 0.0029 1.0GG2 1.0521 1.0806 1.0529 1.0795 0.0276 0.0028 1.0656 1.0520 1.0794 1.0528 1.0784 0.0275 0.0028 1.0654 1.0516 1.0793 1.0524 1.0783 0.0270 0.0026 1.0640 1.0512 1.0770 1.0520 1.0760 0.0268 0.0027 1.0637 1.0507 1.0770 1.0515 1.0760 0.0268 0.0027 1.0637 1.0507 1.0768 1.0515 1.0759 0.0269 0.0026 1.0640 1.0515 1.0766 1.0523 1.0757 0.0273 0.0028 1.0648 1.0511 1.0786 1.0519 1.0776 0.0274 0.0030 1.0652 1.0504 1.0803 1.0513 1.0792 0.0274 0.0030 1.0652 1.0505 1.0800 1.0514 1.0789 0.0276 0.0031 1.0656 1.0506 1.0808 1.0515 1.0796 0.0278 0.0029 1.0660 1.0517 1.0804 1.0526 1.0794 0.0276 0.0029 1.0657 1.0516 1.0800 1.0524 1.0789 0.0276 0.0029 1.0656 1.0515 1.0800 1.0524 1.0789 0.0274 0.0028 1.0652 1.0516 l.0791 l.0524 1.0781 0.0275 0.0028 1.0654 1.0516 1.0794 1.0525 1.0783 10th Percentile CV Warning Limits CT. SA.10 CT + SA.10 0.9819 1.1527 0.9815 1.1521 0.9815 1.1522 0.9814 1.152 1 0.9809 1.1515 0.9804 1.1509 0.9801 1.1506 0.9789 1.1491 0.9786 1.1488 0.9786 1.1487 0.9789 1.1491 0.9796 1.1499 0.9800 1.1505 0.9800 1.1504 0.9803 1.1508 0.9807 1.1513 0.9804 l.1509 0.9804 1.1509 0.9800 1.1505 0.9802 1.1506 Note:

7-day IC1s.. 25% Inhibition concentration. An estimation of the sodium chloride concentration that would cause a 25% reduction In Ceriodaphnfo reproduction (calculated using ToMC~ lc). CT= Central tendency ol the IC1, values S : Standard deviation of the 1c,. values. Control Limits = Mean 1of;larlthrn c 1C1s +/- 2 stanaarct deviations converted to anti-logarithmic value~ Wai 11i11~ limits = Med11 lo11arlthmlc 1c,. ~ 2CV or s._10 converted to anti-logar thm*c values.

s. 10 ~ Standard deviation cocrespondin to the io** percentile of cvs reported 1mtionally by USEPA (S, 10 = 0.08).

CV = Coefficlen of variat on. -l'* t: - 0 QJ u (\\1

l E "C

QJ 0

0.........

QJ b.O 0:: *= 0...

0.

I/) +' ~ c: 0 o_ u 0 c: 0 - 0 c: *;; 0 u +:

l I'll "O

0... I'll c.. QI et: 0 0 i::::... C1J i::::

• u 0

E u

15 offspring per surviving female) USEPA Maximum Coefficient of Variation Guidance Criteria(< 42%), NCDEQ Maximum(< 40%)

• --------4----*~--------

_,*------~.----...... --..

~-

USE PA Lower PMSD Bound (>13%) USE PA Upper PMSD Bound Acceptance Criteria(< 47%) Control Reproduction, Coefficient of Variation (CV) or Percent Minimum Significant Difference (PMSD) PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria. Cent ral Tendency (mean Control Reproduction, CV or PMSD) 95% Confidence Interval (mean Control Reproduction, CV or PMSD +/- 2 Standard Deviations) 0 ~ <o ~ Control Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ToxCal Determination Control Reproduction Control Reproduction CV Control Reproduction Test (offspring/female) Test num ber Test date Survival Mean CV MSO PMSO CT 95% Confidence Interval CT 95% Confidence Interval CT (offspring/female) (%) (%) CT

• 25 CT + 2S CT* 25 CT+2S 1

01-12-16 100 31.2 3.3 1.3860 4.4 31.6 28.6 34.6 4.4 2.1 6.8 5.8 2 02-09-16 100 31.6 7.0 1.9910 6.3 31.4 28.8 34.l 4.6 2.1 7.1 5.9 3 02-09-16 100 32.0 5.9 1.6300 5.1 31.3 29.0 33.7 4.7 2.1 7.2 5.8 4 03-08-16 100 29.4 5.4 1.7490 5.9 31.2 28.7 33.6 4.7 2.1 7.3 5.8 5 04--05-16 100 29.3 6.0 1.7920 6.1 31.l 28.6 33.6 4.8

2. 1 7.4 5.7 6

04-05-16 100 32.3 4.9 1.6950 5.2 31.2 28.6 33.7 4.8 2.3 7.4 5.7 7 05-10-16 100 31.6 s.o 1.6360 5.2 31.2 28.8 33.7 4.8 2.3 7.4 5.6 8 06-07-16 100 27.9 2.6 1.7310 6.2 31.0 28.3 33.7 4.7 2.0 7.3 5.6 9 07-12-16 100 32.5 4.9 1.6710 5.1 31.0 28.3 33.7 4.7 2.1 7.3 5.6 10 08-02-16 100 29.9 6.6 2.1010 7.0 30.8 28.2 335 4.8 2.2 7.5 5.7 11 09*13-16 100 29.7 5.9 2.0410 6.9 30.7 28.2 33.3 4.8 2.2 7.5 5.7 12 10-04*16 100 28.9 3.4 1.6720 5.8 30.5 28.0 33.l 4.8 2.0 7.5 5.8 13 11-01-16 100 28.7 4.4 1.8440 6.4 30.4 27.8 33.0 4.7 2.0 7.5 5.8 14 12-06-16 100 29.3 5.1 1.4550 5.0 30.4 27.7 33.0 4.8 2.3 7.4 5.8 15 01-10-17 100 30.5 3.2 1.5240 5.0 30.4 27.7 33.0 4.8 2.3 7.4 5.8 16 02-07-17 100 27.9 8.5 1.8950 6.8 30.2 27.4 33.0 5.1 2.2 8.0 5.9 17 03-07-17 100 29.7 3.9 1.5300 5.2 30.2 27.4 33.0 5.1 2.2 8.0 5.8 18 03-07-17 100 29.9 5.3 1.9180 6.4 30.2 27.4 33.l 5.1 2.2 8.0 S.9 l!:I 04-04-17 100 30.3 4.9 1.9240 6.3 30.2 27.4 32.9 5.1 2.2 7.9 5.8 20 05-02*17 100 31.0 5.9 1.8940 6.1 30.2 27.4 32.9 5.1 2.3 8.0 5.8 Nott!: Conirol Survival= USEPA minimum test acceptabilhy criteria~ 80% survival. Con ol M~an Reprod\\lction = USEPA minimum Jest acceptability criteria~ l5 offsprfng/surv1v1ng female. CV = Coefficient of variation for control reproduction. USEPA maxlmurr V guidance criteria (90'" percentile)< 42%. NCOEQ maximum CV acceptance crlieria < 40%. MSD

• Minimum gnlllcant difference.

PMSD ~ Percem mlnimun significant difference. PMSO Is a meas1-1e of test precision. The PMSO 1s the minimum pNcent difference between the control and treatment that can be declared stali11ically significant In a whole effluent toxicity test. lower PMSD bound determined by USEPA (10'" percenlilel > 13%. Th ~ lower PMSO bound reore.sents a pracUc;il llmtt to th~ sensithtity of the lPsr me1hod ~nn I ~ n.o1 a min1m11m.. rr~ptanrP rrltHia Upner PMSD bound acceptance crlieria determined by USEFA (90'" percentile) < 47~ CT Central t *ndenc~ ot he reproduc1ion, CV or PMSO values. S

• Standard deviatioo ol the reproduction. CV or PMSO values.

Test PMSO 1%) 95% Confidence Interval CT

• 25 CT+ 25 43 7.4 4.4 7.4 4.3 7.3 4.3 7.3 4.5 7.0 4.4 6.9 4,4 6.9 4.4 6.9 4.4 6.9 4.3 7.1 4.2 7.2 4.3 7.2 4.3 7.3 4.3 7.3 4.3 7.3 4.4 7.4 4.3 7.4 4.3 7.4 4.3 7.3 4.3 7.3

. I c:-:::i..-::: Sodium Chloride Chronic Reference Toxicant Test (EP A-821-R-02-013 Method 1002.0) pecies: Ceriodaplz11ia dubia Page 1 of6 CdNaCJCR #: 201 Dilution preparation information: CommeTtts: NaCl Stock INSS number: INSS \\S gc;, Stock preparation: I 00 g NaCl/L: Dissolve :50 2 N3CI in 500 mL Milli-Q water. Dil11tinn fll'Cf'I (m2/L) 600 800 1000 1200 1400 Stock volume (ml) 9 12 15 IS 21 Diluent volume (ml) 1491 1488 1485 1482 1479 Tot~ I volume (mL) 1500 1500 1500 1500 1500 Test onwnism source i11for111ntio11: Test i11for111ntio11: Organism :ige: I < 24-hours old Randomizi1111, template color: ~ Daie and limes organisms were born I o~*cn*n o'.'\\S TI O'\\C.O Incubator number and shetr between: location: 1.&\\ Culture board: ~-1.* lS*\\"'\\ ~ Replicate number: I ? J ; I

• I s I 6 I 7 f a I 9 I 10 YWT batch:

Cullure board cup number: "'!> "-\\ I '"'\\ I \\'O I \\ ~ l° I \\'\\ f'\\."\\. l't._" J ~ oc.\\*'ll* \\"'\\ Transfer vessel infomiation: pH= '"'\\. l$$ S.U. Temperature= "\\S. \\ *c Sele11as1rnm batch: Os.{)\\*I\\ Average transfer volume (mL): < 0.25 mL D"ilY reneivnl i11formatio11: Day D:it.e Test initfation :ind feed ing, MHSW An:llyst renewal and feeding, or b:itch used termination time 0 05-02-17 0°\\\\\\ o~*1Jo*r1 A ,rt I 05-03-17 o"\\.*1.la*\\'\\ ~ \\J' ot\\"L- \\.-( 2 05-04-17 en'\\ O~*U..,

• l"\\ ~ xc*

3 05-05-17 0 2 'U:> o..\\* u.-11& ~ 4 05-06-1 7 Ol"l.S l'i.<*n*n }( 5 05--07-17 o&7.:0 c 'St* Q'1. - \\ "'\\ xi" 6 05-08-17 o..t l \\ O~*Ol.*\\'1 X\\ 7 ~ 05 17 Oil"'l.. Control inform.ntiou: Acceptance criteria Summmy of test endpoints: % of Male Adults: ()1. S20% 7-day LC50 .., t'-too % Adults having 3rd Broods: I rY\\1~ 2: 80% NOEC (COO % Mortality:

01.

s20% LOEC t-, /'\\('\\ Mean Offspring/Female: ~l-0 <!: 15.0 offspri11g/fcmale ChV tl'IC1.S. 5 %CV: <;:.'\\ 1. <40.0% IC2s (OC.,'\\. l Page 86of94 SOP AT14-Exhibit ATl4.I, revision 11-01-14 ~ p C'"":J l, - p ~ ',_. ~ Species: Ceriodaplmia dubia CONTROL Day I I Voung protlucctl (} Atlult mortnlity 2 Young protlucctl 0 Atlult mortality t_ 3 \\'oung produced 0 Atlu!t mortnlity L 4 Young producctl Yo Adult mormlity 5 Young protlucctl l 't> Atlult mortality L 6 Young protluccd 0 Adult mortnlity \\_ 7 Young producctl \\\\., 2 () c 0 c; \\~ ~ 0 ~s Total )'Oung producctl ~b '°?)"I;) Finni Adult Mortality X for J'd Brootls ')(_ Page 2 of6 CdNaCICR #: 201 Survival aurl Reproduction Dnta Replicate number 3 4 5 6 7 8 9 IO 0 r-i 0 0 0 () A (') L L () ("\\ ri () 0 0 0 0 l_ () ("') b 0 0 0 0 0 \\..- L L \\...... L "-\\ s "'.\\ "-\\ lo..\\ '-{ s s L \\.,_ l\\ V"t. "l. ~ "l. \\ ' I\\ 10 L L L 0 0 0 0 u 0 0 0 \\_. \\..__. L \\,.,.. \\ c:f IS \\'\\ \\"" \\~ \\~ \\ \\o \\ \\.. ~'l. ~\\ ~ll) "?> '1. "?..~ 1.~ ~1. ~' \\.._. \\._ ~ '- )(._ ~ ~ 'X. r-- ")<_ '>£. v Note: Adult mon~lity (I..~ live, D =dead), SB = split broocf (sine lc hrnorl ~ p l i1 he1wecn two dnys}, CO = carry over (offspring carried ov::r with adult during transfer). Co11ce11tration:

1.

600 muNaCl/L Survival and Reproduction Data Replicate number Day I 2 3 4 5 6 7 8 9 10 I Young produced 0 A 6 6 0 0 0 0 0 0 Adult mortality L \\_ L L L. \\.._. L \\... 2 Young prol.luccd ("""I n a D (') b 0 a a 0 Adult mortality L. \\_ \\,_ \\.._ J Young produced (') r> 0 0 b 0 0 0 0 0 Adult mortality \\... \\..- \\... \\... L \\._ L .j Young produced 4 5 s s s '-\\ s s ~ Atlult mortality \\_ '- L L-L- \\._ L \\.__. L_ 5 Young protluccd 't) l.:. \\0 ',_ \\t_ IC '1... lb l \\ \\ \\ Adult mortality L L \\.._ L L L L I._. G Young produced () 0i 0 (') 0 () 0 u ri ('") 1 dull mortali ty \\._ ~ L.. L L L. '- 7 Young produced ,5 >S 11 I~ \\'-\\ l \\c. \\S l~ I°& \\\\a Total young produced (.'\\ ~~ ~,_ ~b ~o ~\\ ~\\ ~~ "'2.C\\ ~~ Finni Adult Mortality '\\_. \\.._ L \\.._. \\..__. \\._ Note: Adlllt mortnlity (L = live, D =dead), SB= split brood (single brood split between two days), CO

• carry over (offspring carried over with adult during transfer).

Co11ce11lratio11: % Mortality: 01 Mean Offspring/Female: .3l. '2- % Reduction from Control: -o.to I. Page 87 of94 SOP AT 14 - Exhibit ATl4. l, revision l l-Ol - l4 l ~ r[=J -- ~ r;:::J ,. ~lol1"Milio)W..-..:.""'- Species: Cerio1/ap/111ia dubia 800 m!! NaCl/L Day I I Young produced () Adult mortality 2 Young produced ('1 Adult mortality L 3 Young produced l'J Adult mortality L 4 Young pr01luccd '-l

\\dull morl:ilit)*

L 5 Young produced \\ \\. Adult mort:ilily l-G Young produced h Adult mortality 7 Young produced '~ 2 0 \\..... !'I 0 \\......_ ~'- r'-. Total young produced !:lo '-"' Finni Adult i\\lorlality \\.._ Page 3 of6 CdNaCICR #: 201 Survival mu/ Reprod11ctio11 Data Replicate number 3 4 5 6 7 8 9 JO 0 D b D 0 0 (') 0 ~ '-- L b 0 0 0 0 0 0 0 \\__ \\__ L L 0 CJ {) t) {) 0 0 0 \\.._. '- L-L.. 5 s ~ 5 "-\\ "-\\ '-\\ \\._. \\........ \\\\ tD \\0 l '?> \\ '1.. \\ "L,,_ \\~ L ("") {') () 0 0 0 0 0 \\._ \\_ \\..... L L.. ,~, \\ i \\ \\.\\ ,5 \\::, ' CS),, \\ '- ~t> 1>>~ ,_~ ~~ '"l.. '\\ ~\\ ~~ ~3 Note: Ad11Jr monnlity CL = live, D = dead), SB = split brood (single brood split bctween two days). CO = carry over (offspring c~rried over with ad11ll d11ring lrnnsfcr). C011ce11tratio11: % Mortality:

01.

Mean Offsoring/Female: ~.'\\ % Reduction from Control: o.~1. 1000 mg NaCI/L Survival mu/ Reproductio11 Data Replicate number Dny l ~ 3 4 5 6 7 8 9 10 I \\loung producctl 0 (\\ D 0 n 0 0 0 0 0 Adnll morialiry \\.._. L L L L.. L L 2 \\'oung protluccd (") 0 () 0 0 (") () a 0 0 Adull mortality L L L L.. L L \\....._ L.. 3 Young protluccd (} 0 0 {") 0 0 0 0 0 0 Atluh mortality \\...- \\.... '- \\_ \\..._ L L 4 Young producctl '"'\\ s '.\\ ~ '-\\ "--\\ '-\\ \\..\\ '-l Adult mortality L-- \\..- L L-L \\...- 5 Young prollucetl l\\ l\\ 10 n...... ~o \\\\ '"L \\-,_ 1\\ ~ '-- AtluJt morrnlity L L \\.... \\..... L \\... G Young producctl n f) (') (', 0 0 0 0 0 0 Atlul! murl:ility \\... \\.- '\\_. '- L \\..... '- \\.... 7 Young pro.ducctl \\ :!> \\ L. \\S \\ <; \\.~ \\ \\... \\~ I~ 13 'S Tot.al young procluccd t.% "tJi L.'\\ ~(:) u ~\\ 3C 1..~ ~\\ finnl,\\dull Mortality L-L L- \\.... \\_ \\....._ \\....._ L Note: Aclull monalily (L = live, D = dead), SB = splil brood (single brood split between two days). CO= carry over (offspring cnrriecl over with adull during transfer). Co11ce11tratio11: % Mortalitv: % Reduction from Control:

s. i 1.

SOP ATl4 - Exhibit ATl4. I. revision 11-01-14 Page 88of94 l Page 4 of6 Species: Ceriodaplmia dubia CdNaCICR #: 201 1200 mg NaCl/L Survival n11d Reprod11ctio11 Data Replicate number Day I 2 3 4 5 6 7 8 9 JO I \\loung produced () b 0 0 0 () 0 0 0 (') Adult mortality \\..... '- L \\..._ \\._ '- 2 Young produced 0 a 0 n 0 0 ('\\ 0 a 0 Adult mortality \\.... 3 Young produced 0 0 a 0 ri 0 ri {\\ 0 C"I Adult mortality \\.._ '- L.. '- L \\._ 4 \\'oung produced ~.,_ ~,_ '-\\ '-\\ ~ 'b '--\\ Adult mortality \\.._ \\...... L.. \\._ 5 Young produced ".\\ lD c;. s ~ IS 5 \\.. ~ '"\\ Adult morlality \\... \\.... \\.... G Young produced D 0 0 0 0 0 D 0 0 D Adult mortality \\.... \\.... \\... L- \\..._. L L 7 Young produced \\ lo s L.. '4 ~ \\o \\..\\ Totnl young produced \\ "'L " '~ \\ '4. l \\ \\g \\1. \\ 1.. \\ 1. \\ S Final Adult Mortality \\.._ Note: Adult mortality CL= live. D =dead}, SB = split brood (single brood split between two days). CO ~ carry over (offspring cnrricd over with adult durin; transfer). Co11ce11tratio11: % Mo1tality: 0 1. / Mean Offspring/Female: \\ 1..'L % Reduction from Control: (o().\\r:..,. 1400 mg NaCl/L Survival mul Reprorl11ctio11 Data Replicate number Day I 2 3 4 5 G 7 8 9 10 l \\'oung produced () 0 0 0 0 0 () 0 0 n Adult mortality L L L.. L L l 2 Young produced 0 0 (') ("\\ ri (\\ 0 0 a ~ Adult mortalil)' L L L L. \\....... L 3 Young produced ll (\\ 0 D 0 (', 0 0 CJ () Adult rnortnlity L \\._ L. L L L \\........ 4 Young produced \\ L... "'L \\ ~ \\ ""l. ~ ~ ~ Adult mortality L- \\._ \\....... L L. 5 Young produced 0 a 0 0 \\ ("') 0 ("\\ 0 Adult mortnli ty \\..._ \\.._. L \\.... 6 Young produced 0 f'I ( ) (') b 0 0 0 0 Adult mortality L- \\...... L-L- '-- 7 Young produced "'l.. 0 () 0 D \\ () 0 () Totnl young produced ~ L. ~ to L. "b s ~ ~ Final Adult Mortality \\.._.. L-L- L \\....... ~ Note: Adult mortality (L =live, D ~ deod}, SB= split brood (single brood split between two days), CO

• carry over (offspring carried over witl1 adull during transfer).

Co11ce11tratio11: % Mortality: 0 1. Mean Offspring/Female: ~~ "2.. % Reduction from Control: 8'1.11. Page 89 of94 SOP AT14-Ex.hibit ATl4.l, revision I l*OT-14 ~ (Q (I) co Q 0.... co ~ '\\ Environmental Testing Solutions Inc. Cont rol Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 5 4 5 10 13 12 6 0 0 0 7 16 15 12 Total 30 33 34 600 mg NaCl/L Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 5 s 5 10 13 10 6 0 0 0 7 15 15 l l Total 29 33 32 800 mg NaCl/L Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 3 5 5 11 12 1l 6 0 0 0 7 15 14 lL Total 30 29 30 Replicate number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 4 4 4 11 12 12 12 0 0 0 0 15 14 16 13 31 30 32 29 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 4 5 4 12 12 10 12 0 0 0 0 13 14 16 15 30 30 31 31 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 4 5 4 10 10 13 12 0 0 0 0 18 14 15 13 33 28 33 29 Verification of Ceriodaphnia Reproduct ion Totals 1000 mg NaCl/L Total 8 9 10 Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 s 44 4 4 5 4 3 3 4 4 4 4 4 39 11 11 10 114 5 ll 11 10 12 10 11 12 12 11 12 112 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 l3 16 16 152 7 13 12 15 15 15 16 13 14 13 15 141 28 32 31 310 Total 28 28 29 30 28 31 29 30 28 31 292 1200 mg NaCl/L Total 8 9 10 Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 0 I) 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 5 6 48 4 3 2 2 4 2 4 4 3 3 4 31 13 11 11 114 5 4 6 5 5 3 8 5 6 3 7 52 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 16 13 16 150 7 5 1 6 5 2 4 3 3 6 4 39 34

29 3J 312 Total 12 9

13 14 7 16 12 12 12 15 122 1400 mg NaCl/L Total 8 9 10 Day Replicate number Total 1 2 3 4 s 6 7 8 9 10 0 J 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 J 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 42 4 l 2 2 1 3 l 2 3 3 3 21 12 12 13 116 5 0 0 0 2 0 1 0 0 0 0 3 0

J 0

0 6 0 0 0 0 3 0 0 2 0 0 5 15 17 16 151 7 2 0 0 0 0 0 1 0 0 0 3 31 33 33 309 Total 3 2 2 3 6 2 3 5 3 3 32 .......... *r Environmental Testing Sol.. tlons. Inc. Concentration (mg/l NaCl) l 2 Control 30 33 500 29 33 800 30 29 1000 28 28 1200 12 9 1400 3 2 Ounnett's MSO value: 1.894 PMSO: 6.1 3 34 32 30 29 13 I. 4 31 30 33 30 14 3 MSD = PMSD ~ Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Replicate number Survival 5 6 7 8 9 10 (%) 30 32 29 28 32 31 100 30 31 31 34 29 33 100 28 33 29 31 33 33 100 28 31 29 30 28 31 100 7 16 12 12 12 15 100 6 2 3 5 3 3 100 Minimum Significant Difference Percent Minimum Significant Difference Test number: CdNaCICR 1#201 Test dates: May 02-09, 2017 Average reproduction Coefficient of Percent reduction (offspring/female) variation (%) from control (%) 31.0 S.9 Not applicable 31.2 S.6 -0.6 30.9 6.4 0.3 29.2 4.2 5.8 12.2 21.8 60.6 3.2 41.1 89.7 PMSO is a measure of test precision. The PMSO i> the minimum percenl difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10'h i;ercentile) = 13%. Upper PMSO bound determined by USE PA (901h percentile) = 47%. Lower and upper PMSO bounds were determined from the 10th and 90th percent I, respectively, of PMSD data from EPA's WET lnterlaboratory Va riability Study (USE PA, 200la; USE PA, 200lb). The lower PMSO bound represents practical limit 10 the sensitivity of the test method and Is not a minimum cceotance criteria. IJ<;f PA 700111, 200lb. ~in*I Report: lnterliboritory Va rlablllty Study of EPA Short-term Chronic ~nd Atutc Whole Effluent Toxicity Tes1 Ml!thods, Voluml!s 1 and 2*AppendD:. [ PA-821-8-01-004 and EPA-821-11-01-005. US Environmental Protection ARencv, Cincinnati, OH. Statistical Analyses tnwlronm*fftal THtlnQ SoluUonl. IM. Ceriodaehnia Survival and Reeroduction Test-Reeroduction Start Date: 5/212017 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 51912017 Lab ID. ETS-Envir Testing Sol. Sample Type: NACL-Sod1um chloride Sample Date. Protocol* FWCHR-EPA*621*R*02*013 Test Specie CD-Cerlodaphnia dvbia Comments: Conc-m51L 2 3 5 6 7 6 9 10 D-Conlrol 30.000 33.000 34.000 31.000 30.000 32.000 29.000 26.000 32.000 31 000 600 2.9 000 33.000 32.000 30.000 30.000 31 000 31.000 34.000 29.000 33000 600 30.000 29.000 30.000 33.000 26.000 33 000 29 000 31 000 33.000 33.000 1000 26000 26.000 29000 30.000 26.000 31.000 29.000 30.000 26.000 31 000 12.00 12 000 9000 13.000 14.000 7 000 15.000 12 000

• 2.000 12.000 15 000 1400 3.000 2.000 2.000 3.000 6.000 2000 3.000 5 000 3000 3.000 Transform : Untransformed 1-Tailed Isotonic Conc-ma1L Mean N-Mean Mean Min MaJC CV%

N !*Stat Critical MSD O.Conlrol Jl 000 1.0000 'H.000 2~.000 34 onn 5 8 11!'1 10 600 31.200 1 0065 31.200 29.000 34.000 5,613 10

• 0.241 2.267 1.894 800 30.900 0 9968 30 900 28.000 33.000 6.373 10

0. 121 2.267 1.694 1000 29.200 0.9419 29.200 26.000 31.000 4.210 10

2. 173 2.267 1.894

• 1200 12.200 0.3935 12 200 7.000 16.000 21.790 10 22.694 2.267 1.894 "1 400 3200 0.1032 3.200 2.000 6.000 41.143 10 33.557 2.287 1.894 Auxilia!}'. Tests Statistic Crlllcal Kolmogorov D Test indicates normal distribution (p > 0.01 )

1.00629 1.035 Bartlelt's Test tnd1cales ~uat veriances !E = 0.23! 6.90044 15.0663 H~eothesls Test (1 *tall. 0.05) NOEC LOEC ChV TU MSDu MS Op MSB MSE Dunnett's Test 1000 1200 1095 45 1.89434 0.061 11 1481.51 3.43146 Treatments vs 0-Control Linear Interpolation (200 Resample!O Point mgtL so IC05 959.412 63. 1306 IC10 1014 24 8 4 1223 IC15 1032.53 5.7 1313 JC20 1050.82 5.39734 lC25 1069.12 5.25268 IC40 1124 5.8972 ICSO 11 60.59 7 02907 950/o Cl 779.216 005.53 999 237 1023.54 1018.69 1041.09 1036.27 1056.92 1057 36 1077.82 1110.95 11 35.01 11<15 4 1173.15 Skew -1.2229 -3.6237 .() 0750 -0.0191 0.0038

• 0.0715

- 0.0600 1.0 0.9 0.8 0.7 "' 0 6 ~ o.s ~ 04 er:: 0.3 02 0 1 0 0 c.-----<1-.(Y"" Mean 31.100 31.100 30.900 29.200 12.200 3.200 Skew -0.1735 F-Prob 3.2E-42 0 1 -~~~~~~~,....,.~~~~ 40 35 30 g 25 u 15 20

c.

~ 15 10 5 0 ~

c.

0 u 0 Page 92of94 0 Dose-Response Plot 500 1000 Dose mg/L 1-lail, 0.05 level of significance 1500 N-Mean 1.0000 1.0000 0.9936 0.9369 0.3923 0 1029 Kurt 0.13786 df 5, 54 l j J Page 5 of 6 Species: Ceriotlaplmia dubia CdNaCIC R #: 201 Daily Cltemist1y: Day (Analyst identified for each day. performed. pH. 0.0. and conductivity measurements only.) 0 l 2 Anal)*st II-J-.1 .L ~ Concentration Parameter r ~ pH (S.U.) 8.2.l 6.o~ S.*o 'b.02. e.oe '\\A~ DO (111g/L) ")._q 1-~ ~.'l- ~ "lr ~.e Conductivity ,32..1 3'2<3 ~lct (µmhos/cm) l CONTRO L

• Alkalinity jl' -

~"'" ~** (m~CaCO;/L) lo\\

• *.. <j*

la"'Z. .-~~ *l

• lln rdncss

<(g r~I!. g-i. (mg CaCOi/Ll I (. "Temperature ( 0C) L."\\.lo l.C:.. \\ "'-\\.\\."'\\ 1...&.\\.E "'l.'\\. 8 "'\\.'\\."\\ pH (S.U.) t)) 1<; ~-'l\\4 e.o'-1 ~~~ {JJ.L)~ ~q\\.\\ DO {mg/L) "1..8 ~.<3 '\\...+ ~-q 1-:~ 1'-8 600 mg NaCl/L Conducth*ity 14~0 l?AO t3~o (µmhos/cm) "Tempernture (°C) &.'-\\* ""'\\ 1..\\..\\- ~ "'\\.'-\\*1 '""\\.~. f U\\*"l 1.'l*~ pH (S.U.) 0.\\"' ~q3 A.01. "iO.-s t!>Cz. 'H\\\\ DO (mg/L) 'l~ '}.0 '.:\\+. ~.'1 -:l':\\- 1-.q 800 mg NaCl/L Conductivity l1=tQ l":t-0 11-t10 (1m1hos/em) "Tcm pernturc (°C) '1.."-\\* \\ "\\S.O "\\_'-\\. i "1.~l."\\ 1..'-l* 1. "l.'\\.t pH (S.U.) eJ..\\3 ~cu e>.oZ '-tAlO A.oz. 1:\\...(3 DO (mg/Ll ":l: a l.},A

• ~.a

":\\,.q 1:.e'J 't.'l 1000 mg NaCl/L Conductivity ~,. (µmhos/cm) 2.\\30 '-.000 ~05'0 ~

• Temperature (

0C) '1..'-1.* 1 "\\.J. *.i "l'\\.* g 'N."\\ -i.'-\\* 1 L.'\\.f pH (S.U.) e.,z.. '+.<:\\( eoa ~~'"' e.oz.. 4.01.P DO (mg.IL) ~-<3 r.}a ~q ~q ~e 't.~ 1200 mg NaCl/L Con<luctivlty (umhos/cm) a4so ~'110 ~'4.30 "Tempernturc (°C) l.."\\.1 1.'-\\. f "'\\.'\\ *.f -i.s.o 1.'-1.*'\\ 1!-i. t pH (S.U.) 0.12- ~~* 6.01 ~!\\&.{ ~.01 't.e"' DO (mg/L) e>.o ~<:!\\ 0o 8.o '+<'.\\' /}~ 1400 mg NaCl/L Con <l udi vi~* °'1'10 ~-:t'-ID IA 'l 1<J (µ mhos/cm) \\

• Temperature (°C)

'"'l"\\*1 "1.-.\\.. f i.t.1.. e ,,~. '\\ "l '{." \\_q.~ Initial Final Initial Final Initial F inal

• Temperatures pcrfonncd at the time of test initiation, renewal or tcrminmion by the analyst idcnti!icd in the Daily Renewal lnforniatio'l tablc located on Page I. Alkalinity and ~rdncss pcrfonncd by the analyst idcntilic<l on the bench sheet specific for cnch analysis and transcribed Lo this bench sheet by:
• ~

Page 93 of94 SOP AT l4 - ExhibitATl4. !, revision 11 14 'j I I I l l 1

1~ ~ r=P Page 6 of6 J

Species: Ceriodaplmia dubia CdNaCICR #: 201 Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 3 4 5 Analyst µ Q..::. """' °""...... .J.-- I)..., lr' .u Concentration Parameter .~ ~- pH (S.U.) 0.\\3 'iL!>~ ~.o'f, 'l~ ts J'S e.uo. e.\\l\\ DO (mg/L) +_-:}- +.'l ';/,Ci. 1-.6 r..a.,. ll'l '~.C3 Co111Juctivity 32'-l 3ZD

Tu

.31-:r-(um hos/cm) ~. -

• Alkalinity

-~ -* ~.,~" - ~~i'\\ CONTROL ~~" '-~ (m~ CaCO,IL) <1l

• nanlne.ss

- 6~.. 86 (mg CaCO;IL) "Temperature L.."'-\\.l '"l.S.'L. '"\\.~. '\\ "l.."\\ ~ ""1..1.\\*1 '1.S*\\ 1."\\.& c 0c> pH (S.U.) A.OS +.Ar "t~ '\\Al a.o-6" 0,0\\ e,.\\\\.\\ DO (mg/L) "'4:~ 1-.'l -:i.. 'l ~ 1-6 /.\\.ll ~.a Con<lucrivity ~ 600 mg NaCl/L l'260 14\\0 ' *400 \\~2.0 (µmhos/cm) ~

• Temperature

,_"\\~ '1..S.1.. Vo\\!\\ "\\S..O 1.."'.*1 ~4..& ).i.\\.'=\\ c 0c> pH (S.U.) 0.0-; 1-}\\~ i ~'1'- '+~\\.\\ a.o~ e.o2 0.\\3 DO (mg/L) r-l-.'1 ~..o ~. o 'l,,q ".}.~ ~A ~-~ Con<lucrivity ~:f40 ~ 800 mg NaCl/L 1'1*30 l1":20 1150 (umhos/cm) "'Temperature '1..'-\\ *~ 1..s.o \\S.o '1.S-0 '1.."\\* 1 '\\.."\\..(I- 'l~-4'. C"C) pH (S.U.) e.03 ~A~ "'6.0111 /f?\\ I 0.0lP Af\\l (2),\\3 DO (mg/L) ~-nq ~,\\ ~.c /}J:} I'.\\ e ".\\-.q '1-.0 1000 mg NaCl/L Conductivity ao~ ~uo C1rn1hos/cm) a\\OO ~l2>0 "'Tempera ture '1.c.( *.f -v\\. '\\ LS.o l,S.Q 1..-\\.f l.."l.'\\ "'l."(. ' c*ci pH (S.U.) ,q.02. +.C\\<i. "&.o.., 4!10 ~Dt.p '+-.C\\Gl 0.l3 DO (myL) e.o "i :1... 'lSo E!).D ~-q ~o '-t,q C n<luctivity ~ 1200 mg NaCl/L ti430 .;\\%t> ~'460 - a'{(t)O (~m1hos/cm)

• Temper:iturc

'l.~*f '-~* \\ "1$.0 15.-~ 'lo\\ *4' 'lS-1.. °l."l*'i ("C) pH (S.U.) ~..tn. 1-.'t.~ K~ ~.qo 0.05 6.IY'> e.r~ DO (mg/L) B~o ~.'1... +.JI( e?..V ~o ~.o a.* Con<lucth<ity 1400 mg NaCllL )i1-1o

\\'lo.;

,

A"i'10 ?.610 (11mhos/cm) ~ "Temperature 1."l.1 '1.~* g is.o 1...'i -~ 1.'-l-'\\ 1,."\\... '( 1.'-\\.<\\ (°C) In itial Final Initial Final Initial Final Initial "'Temperatures performed at the time of test initiation, renewal or 1crminn1ion by the analyst identified in the Daily Renewal lnfommtion table located on Page I. Alkalinity and hardness performed by the onal)'st identified on the bench sheet spcci fie for each analysis and transcribed to this bench sheet by: ~ - Page 94 of94 SOP AT1 4 - Exhibit AT 14. 1, revision I 1-0 1-14 6,,_ A.\\3 t.A -~ J ~ - ~ ~

...:;!:~

"\\.'$. t> e.o3 '\\-. c=t

• -'. '~.

"'l.S.. () e.1)2... '} *.::t. ,I -=- ,_.. \\. ~ ~IV> g,o "'2,.'{. & 1-~q e.o ~.-. - "t

"I

'1...\\. '\\ 'lAe Q*O ~ L.'S. 0 Final ~}}